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BIO-2500 Syllabus MW Spring 2011


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									                                 Microbiology (BIO-2500)
                                 Course Syllabus – Spring Semester 2011
                                Lecture Section: 10241 [Room ESS-2102]
                               Laboratory Section: 10243 [Room EHCT-240]

Instructor: Steven Leidich, Ph.D.

Office: Room EHCT-211B
Phone: 216-987-2394
Office hours (EHCT-211B):              M/W: 9 – 10 AM, 3:00 – 4 PM.
                                       T/R: 9 – 10:30 AM, 3:00 – 4 PM.
                                       F:   9 – 9:30 AM, 12:30 – 1 PM

Course Description: BIO-2500 is a survey of microorganisms in terms of physiology, biochemistry,
genetics, and diversity. Methods of control including physical, mechanical, chemical, and
chemotherapeutic will be discussed. Emphasis is placed on prokaryotes and eukaryotes causing human
diseases and the role of the immune system in response to these microbes.


     Lecture:      Microbiology: A Human Perspective. 6th edition. 2009. Nester, E.W. McGraw Hill.

     Laboratory: Microbiology Laboratory Theory and Applications. 2nd or 3rd Edition. 2006. Le Boffe
                 M.J., and Pierce, B.E. Morton Publishing, Englewood, CO.

Materials: Protective laboratory safety glasses/goggles, are required for all students. These are
available in the bookstore. An old shirt is recommended for lab.

Attendance: Students are expected to attend all lecture and laboratory meetings. If you must miss class,
you must arrange to get any notes, handouts or other lecture material from your classmates or by visiting
the supplemental site ( discussed the first day
of class. I will not provide this information beyond the lecture that it was initially distributed. Additionally,
students are responsible for learning what they may have missed. “Not knowing” about a topic because
you were absent is not an excuse for failing to be prepared for tests.

Performance Objectives: Upon successful completion of the lecture portion of BIO-2500, the student
should be able to demonstrate knowledge of the structure and function of pathogenic and non-
pathogenic microorganisms, and should understand the role of the immune system in protecting the body
against microbes. Upon completion of the laboratory sessions the student should have developed the
proper techniques required to culture, stain and identify microorganisms and have developed proficiency
in the use of oil immersion microscopy.

Classroom Conduct: BIO-2500 is a demanding Sophomore-level college course - you will be expected
to act and perform at this level. Students are asked to turn off/silence cellular phones during lecture and
exams. Food and/or beverages are strictly prohibited in the laboratory. Children are not permitted in the
lecture or laboratory. Students may bring a drink but no food to lecture.
        Attendance is required during all scheduled lab sessions. Attendance will be taken at the
beginning of each lab. Arriving late is disruptive, shows a lack of commitment, and is disrespectful
to your classmates and your instructor. BE ON TIME!!

GUM: If you choose to chew gum, please do so quietly (no popping).
Tests and Grading Policies:

In order to pass BIO-2500 you must earn at least a 60% in both lecture and lab.

Lecture: There will be 5 lecture tests each worth 100 pts and a final test worth 50 pts. The final test will
be comprehensive and must be taken (it will not be dropped). The lecture grade will represent two-thirds
(66.6%) of your overall course grade.

Laboratory: There will be five laboratory quizzes. The last lab quiz (40 pts) is comprehensive and must
be taken (it will not be dropped). The lab grade will also include the preparation of slides with proper
staining techniques. The lab grade will represent one-third (33.3%) of your overall course grade.

Cheating: Any student who gives or receives answers on a test will receive a grade of ZERO for the
exam and will be reported to the Dean of Student Affairs.

Make-ups: There are NO make-up tests. Your lowest test grades in lecture and lab will be dropped.

The grading schedule for BIO-2500 will be as follows:

A = 90-100%
B = 80-89%
C = 70-79%
D = 60-69%
F = Below 60%

Learning Resource Center: Tutors for BIO-2500 are available in room ESS-1108.

Students with Disabilities: If you need course adaptation or accommodations because of a disability,
have emergency medical information, or need special arrangements in case the building must be
evacuated, please inform the instructor as soon as possible. Every effort will be made to accommodate
any special needs that students may have.

Note to the Student: Each and every one of you has the ability to successfully pass this course with a
good grade! As your instructor, I am here to help you in any way that I can. Please, if you have any
problems, concerns, need extra assistance, or have suggestions, please do not hesitate to contact me.

Note: The instructor reserves the right to amend, revise, or correct this syllabus with proper notification of
the students enrolled in the class.

                   TENTATIVE BIO-2500 LECTURE SCHEDULE
                             Spring 2010 (10241)

    WEEK   DATE                                 TOPIC                                 READING
     1      1/19     Introduction
     2     1/24      Introduction to Microbiology                                Chapter 1
           1/26      Bacterial Cell Structure                                    Chapter 3
     3     1/31      Cont’d                                                      Chapter 3
            2/2      Bacterial Growth and Metabolism                             Chapters 4, 6
     4      2/7      DNA, Transcription and Translation
            2/9      DNA, Transcription and Translation (cont’d)
                     Start Bacterial Genetics                                    Chapters 7-9
     5     2/14      TEST 1 (Introduction to Microbiology – DNA, Transcription
                     and Translation)
           2/16      Bacterial Genetics (cont’d)
     6     2/21      Immune System 1                                             Chapter 15
           2/23      Immune System 2 – Humoral and Cellular                      Chapter 16
     7     2/28      Immune System (cont’d) - if needed
            3/2      Host Microbe Relationships                                  Chapter 17
     8      3/7      TEST 2 (Bacterial Genetics, Immune System, and Host
                     Microbe Relationships)
            3/9      Microbial Control                                           Chapter 5
     9     3/14      Spring Break (No Class)
           3/16      Spring Break (No Class)
     10    3/21      Antimicrobial Therapy                                       Chapter 21
           3/23      Antimicrobial Therapy (cont’d)
     11    3/28      TEST 3 (Microbial Control, Antimicrobial Therapy)           Chapters 13, 14
           3/30      Virus Structure and Replication                             Chapters 13, 14
     12     4/4      Virus Structure and Replication (cont’d)
            4/6      Survey of Viral Diseases (HIV)                              Chapters 29
     13    4/11      Survey of Viral Diseases (HIV) (cont’d)
           4/13      Survey of Bacterial Diseases                                Chapters 22-28
     14    4/18      TEST 4 (Virus Structure and Replication and HIV)
           4/20      Survey of Bacterial Diseases (cont’d)
     15    4/25      Survey of Bacterial Diseases (cont’d)
           4/27      Fungi                                                       Chapters 12 and 17
     16     5/2      TEST 5 (Bacterial Diseases and Fungi)
            5/4      Review
           5/11      Final (Comprehensive) [9:15 – 11:15 AM]


                          Spring 2010 (10243)

Week   Date                      Topics
 1     1/19    Introduction and Safety: (pp.1-5)

 2     1/24    Culturing the Environment: Exercises: 2-1
               Handling Cultures: Exercises: 1-1, 1-2, 2-2

       1/26    Growth Characteristics: Exercises: 2-2, 2-3, and 2-4
               Microscope: Ex. 3-1

 3     1/31    Streak Plate Isolation: Exercises 1-3

       2/2     Gram Stain: Exercises: 3-4 and 3-6
               Bacterial Identification: Textbook Chapter 10

 4     2/7     Gram stain unknown: Exercises: 3-12       (10 pts)

       2/9     LAB QUIZ 1                                (20 pts)

 5     2/14    Pour Plate technique and Plate Count: Exercises: 6-1, App. C

       2/16    Endospore Stain: Exercises 3-9            (5pts)
               Acid Fast Stain: Exercises 3-7            (5 pts)

 6     2/21    Biochemical Tests: Exercises: 5-3, 5-11, 5-12, 5-13, 5-20

       2/23    LAB QUIZ 2                                (20 pts)

 7     2/28    Multiple Test Systems: Exercises: 5-29 and 5-30

       3/2     Selective Media: Exercises: 4-5 and 4-7

 8     3/7     Potable Water Analysis: Exercise: 8-1 and Handout

       3/9     Gram Positive Cocci: Exercises: 2-8, 4-4, 5-5, 5-25, and 5-27 (read)

 9     3/14    Spring Break (No Class)
       3/16    Spring Break (No Class)

 10    3/21    LAB QUIZ 3                             (20 pts)
               Stan’s Salad Saga: Exercise: 10-3 and Handout

       3/23    Extraction of DNA from Bacterial Cells: Exercise 10-1

 11    3/28    Bacterial Transformation: Exercise: 9-4

       3/30    ELISA: Theory

 12    4/4     LAB QUIZ 4                                (20 pts)

       4/6     ELISA: Experiment Exercise: 11-7

 13    4/11    Disinfectants and Antiseptics: Handout
     4/13   Fungi: Exercise: 11-1(read)
            Fungal plate

14   4/18   Eukaryotic Parasites (Handout);
            Eukaryotic Parasites: Exercises: 12-3 (read) and 12-4 (read)

     4/20   Examine fungal plates

15   4/25   Review

     4/27   LAB QUIZ FINAL                          (40 pts)


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