EFFECTS OF LPS INJECTION ON MICE WITH CHRONIC KIDNEY DISEASE, B. V. Trivedi, T. S.
Puri*, L. Mathew, University of Chicago, Division of Nephrology, Department of Medicine, Chicago, IL
Previous epidemiological data suggests that chronic kidney disease (CKD) is a risk factor for the
development and severity of acute kidney injury (AKI). In this study we sought to investigate whether
properties inherent to the diseased kidney itself account for the observed association between CKD and
AKI. As a model of CKD, we used a reversible unilateral ureteral obstruction (rUUO) method in C57BL/6
mice which results in functional and histopathologic features of CKD. These mice along with controls were
injected with increasing concentrations of bacterial endotoxin (LPS) to induce AKI followed by assessment
of the severity, duration, and recovery from AKI using blood urea nitrogen (BUN) measurements.
Development of AKI was observed in all groups of mice (CKD (n=18,19) versus surgical (n=9,9) and wild-
type controls (n=10,10)) after intraperitoneal injection of LPS at both doses, 0.15mg/25g mouse and
0.25mg/25g mouse, tested. AKI was more severe in mice with CKD at the higher administered dose of
LPS. At both doses of LPS tested our data shows that control mice and those with CKD get kidney injury
for only 72 hours after injection and then return to the same level of kidney function that existed prior to
injection. Our findings suggest that structural and functional changes associated with CKD in our mouse
model do not confer an increased susceptibility to development or duration of AKI after LPS injection but
may have impact on the severity of AKI.