Toe Clip Protocol

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					                            Toe Clip Protocol
1. Toe mouse normally. Save each toe clipping in a marked eppendorf (or in PCR
   tubes).
2. Add 20-30 µl of toe buffer to each sample.
3. Add 2-3 µl of Proteinase K (10 mg/ml).
4. Heat at 65oC for 60-90 minutes.
5. Vortex.
6. Heat at 65oC for 60-90 minutes (this can be done in the PCR machine).
7. Add 180 µl of ddH2O to each sample.
8. Use 1 µl of DNA in PCR dilution.




                           Toe Buffer (10 mls)

                         500 µl of 1M Tris (pH 8.0)
                             40 µl of 5M NaCl
                           20 µl of 0.5M EDTA
                              1 ml of 10% SDS
                             8.44 mls of ddH2O

				
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posted:10/14/2011
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