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									ANTI tTG AUTOANTIBODIES CROSSREACTING WITH BRAIN
  STRUCTURES ARE PRODUCED IN THE INTESTINE IN A
    CELIAC PATIENT WITH EQUILIBRIUM DISORDER
 S. Boscolo1, D. Sblattero1, F. Forian1, R. Marzari1, T. Not1, A. Ventura2,
                               E. Tongiorgi2
     1
         Dept. of Biology, University of Trieste, 2IRCCS Burlo Garofolo,
                         Pediatric Clinics, Trieste, Italy
Introduction: Cerebellar ataxia is the commonest neurological
complication of celiac disease. Methods. We compared on sagittal rat brain
sections the staining pattern produced by the serum of a celiac disease
patient presenting with vertigo (F, 45) and phage-display IgA antibody
libraries obtained from her peripheral (circulating) or intestinal biopsy
lymphocytes. Controls: 10 healthy blood donors, 20 adult celiac patients
without neurological symptoms. Results. At the time of intestinal biopsy
the patient serum was strongly positive for the autoantigen tissue
TransGlutaminase (tTG) and the IgGs stained neurons from Purkinje cells,
deep cerebellar nuclei, brainstem, colliculi, and neocortex. The
hippocampus and the striatum were poorly stained. The serum IgAs
staining pattern was similar although much fainter than with IgGs. No such
pattern was observed with sera from 20 adult celiac patients without
neurological symptoms or from 10 healthy blood donors. The phage-
display IgA antibody libraries from peripheral and from biopsy
lymphocytes showed similar staining patterns although the staining of the
biopsy antibody library was stronger. Anti tTG antibodies were isolated
only from the biopsy library (two different classes), one class was positive
for brain staining. Conclusions. Using celiac disease patient-derived
libraries we show that anti-tTG antibodies produced by lymphocytes
harboured at the intestinal level cross react with specific rat brain
structures.




Abstract Number: 220
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