Coulochem III Electrochemical Detector by qingyunliuliu

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									Coulochem III
Electrochemical Detector
  Sensitivity
  Reliability
Convenience
  Flexibility
                                          TODAY,
                                          FORWARD THINKING


  Performance

                       T H E D E M A N D F O R H I G H LY S E N S I T I V E A N D
      SELECTIVE DETECTION IN HPLC HAS NEVER BEEN
                  G R E AT E R . E L E C T R O C H E M I C A L D E T E C T I O N I S
THE ANSWER WHEN YOU NEED TO MEASURE PICOGRAM TO
                                   FEMTOGRAM LEVELS OF OXIDIZABLE
                                                     O R R E D U C I B L E C O M P O U N D S.


OF ALL THE HPLC DETECTORS, NONE ARE AS SENSITIVE
AND SELECTIVE WITH AS WIDE A DYNAMIC RANGE AS AN
                         E L E C T R O C H E M I C A L D E T E C T O R F R O M E S A.




                                                                     The ESA Coulochem® III detector is the standard in
                                                              high sensitivity electrochemical detection. With its modular
                                                                design, the Coulochem III platform can be used with any
                                                               HPLC system. All new electronics with the latest in digital
                                                               technology guarantees the lowest detection limits possible.
       Principle of Coulometric Technology:
       Compound A is completely reacted at
       Sensor 1 thus eliminating it from detection
                                                              The Coulochem III incorporates DC and Pulse/Scan modes
       at Sensor 2. Compound B passes Sensor 1
       unchanged and is detected at Sensor 2.
                                                              in discreet modules. By optimizing the detector for the low
       The result is that A and B are resolved
       electrochemically.
                                                                  signal in DC, the detector achieves the highest possible
                                                               sensitivity from capillary to normal bore chromatography.
                                                          Pulse mode supports variable timing of potentials and duration
                                                              of waveforms to optimize the application. Scan mode helps
                                                                select the optimal potentials. The Coulochem III detector
                                                                                            offers DC, Pulse/Scan or both.
                         “The ESA Coulochem is by far the most
                          sensitive EC detector on the market today,
                          very reliable and backed by superior
                          technical support. Its sensitivity makes
                          it stand out from the others.”
                                - Professor Timothy Maher
                                  Massachusetts College of Pharmacy
                                  and Health Sciences




Sensitivity
With the sensitivity
and selectivity of the
Coulochem III, peer
into the living brain,
view the mechanisms
of aging and cancer                                For DC mode, each Coulochem III detector comes with
or create a picture
                                               two analytical channels and an additional channel, unique
of health and disease.
                                               to ESA, for mobile phase cleaning or sample pretreatment.
                                            In Pulse mode, you can select potential and duration of up to
                                           4 segments to give the ultimate in stability and reproducibility.
                                        Operate switching valves or signal for external events, change gain
                                           ranges or potentials with Time Line or shut off cells at the end
                                             of a run. All this stored as one of 25 methods for easy recall.

                                                         All standard ESA cell designs can be used with the
                                           Coulochem III detector platform. Expanded gain ranges make it
                                           ready for any cell type. Enhanced processor speed provides data
                                           acquisition fully compatible with today’s faster chromatography.
THE IMPACT OF



Reliability

                RELIABILITY IS ONE
                OF THE KEY DESIGN
                ELEMENTS OF THE
                COULOCHEM III




                           State-of-the-industry electronics guarantee reliability. The proven
                   Coulometric cell design is well known for ruggedness and operating every
                time right out of the box. The solid state reference electrode used in all ESA
                              cells never needs replacing, refilling or any other maintenance.
                                            Hands off, fully automated operation is a reality.


                                   MORE TO RELIABILITY THAN JUST HARDWARE.
                      ESA has been making electrochemical detectors for over 40 years. The
                 name ESA is synonymous with reliable electrochemical detection in HPLC.
                The Coulochem III is backed by superior instrument service and superlative
                applications support by experts that specialize in ECD. ESA electrochemical
                  detectors are used in the most demanding environments imaginable. From
                         nuclear reactors to clinical laboratories – the Coulometric family of
                                                        detectors have proven their reliability.
                                                               LARGE DISPLAY
                                       MODULAR BOARDS          easier to read            OPTIONAL
                                       a choice of modes:      with more                 INTEGRATED ORGANIZER
                                       DC, Pulse/Scan or       useful information        protects columns
                  LARGE KEY PAD
                  easy to see,         both                                              and cells, with or with-
                  easy to use                                                            out temperature control

                                          A CHOICE OF CELLS
                                          current and future      ADVANCED ELECTRONICS
                                                                  reduced noise
            SOFTKEYS
            menus that are
            easy to navigate




Convenience
     The advanced features of the
   Coulochem III platform provide
   performance and reliability in a
 detector that is convenient to use.
  The Coulochem III detector was
       designed by people that use
                    ECD every day.
   THE ENDLESS POSSIBILITIES OF




Flexibility
A CHOICE OF CELLS FOR OPTIMIZATION OF YOUR ANALYSIS



                                                                   The Coulochem III detector comes with a
                                                                 choice of cell designs. From simple analysis
                                                               with amperometric electrodes to sophisticated
                                                                sample manipulation with Coulometric cells,
                                                                   ESA offers the flexibility to save time and
                                                                   effort in optimizing analytical conditions.
                                                                    All ESA cells come standard with a solid
                                                                  state, maintenance free reference electrode
                                                                         known world wide for its reliability.




               COULOMETRIC CELLS – There is no                   PRE-ANALYTICAL CELLS – The high
electrochemical cell more sensitive or reliable. Period.         conversion efficiency of the coulometric
   Proven in thousands of laboratories worldwide, the      electrode when used before the analytical cell
       flow-through design with high surface area and          provides an additional level of selectivity.
 minimized diffusion distance provides 100% analyte        When placed before the injector, mobile phase
 conversion and the highest signal possible. Each cell        can be electrochemically cleaned for lower
  has two working electrodes for maximum analytical             background and enhanced performance.
            flexibility using modes pioneered by ESA’s
    Applications Laboratory. Screen and Redox modes            SPECIALTY CELLS – Some applications
   increase selectivity and reduce sample preparation.       have unique requirements. The 5014B and
  Channel ratioing gives you added assurance of peak       5041 cells are specifically designed and tested
        identity. When downtime is unacceptable, ESA                  for the demands of microdialysis in
                  Coulometric cells are the only answer.       neuroscience where extreme sensitivity is
                                                                required and high sample salt content is
            AMPEROMETRIC CELLS – These single                                                 encountered.
       amperometric electrodes are routinely used for
     capillary or microbore chromatography, in pulse             SYNTHESIS CELLS – Electrochemical
    mode or when metal electrodes are required. This               oxidation can be used to produce drug
  design is used with the ESA Boron Doped Diamond                    metabolites in quantities suitable for
electrode which expands the useable potentials range                    MS, NMR and biological studies.
                          in electrochemical detection.
                  THE COULOCHEM III GIVES YOU FLEXIBILITY
               IN BOTH EXPERIMENTAL AND INSTRUMENT DESIGN

      THAT MEANS YOUR DETECTOR WILL NEVER BECOME OBSOLETE

The Coulochem III detector is a platform for        you can program gain changes, run Clean Cell
the future. It is designed so that you can recon-   or turn off pumps at the end of a method.
figure the instrument now or in the future to fit
your analytical needs.
                                                                 DC OR PULSE: YOU CHOOSE
The organizer module houses multiple
                                                                     WITHOUT COMPROMISE
columns, Analytical and Guard cells and pulse
                                                                           Other Electrochemical
dampers. It conveniently mounts a manual
                                                                                  detector designs
injector. Once inside, cells and
                                                                                   incorporate      the
columns are protected from                                                         high     sensitivity
the environment. The                                                               DC and pulse
organizer can be easi-                                                             components on the
ly removed or                                                                      same boards. This
added      without                                                                 means that the
tools. A complete                                                                  electronics      are
analytical config-                                                               compromised for the
uration can be                                                                 differing requirements
                                                                             of     these    disparate
neatly assembled
                                                                           modes of operation. In
and stored for a
                                                                          the Coulochem III there is no
quick and easy
                                                                        compromise. DC and pulse
change of method.                                                     are on separate boards, opti-
                                                                    mizing the electronics for each
OPERATE EXTERNAL DEVICES                                           mode.
                                                    When DC or Pulse mode is needed, simply add
The Coulochem III detector allows operation of
                                                    the specific board for a fully optimized detector.
external switching valves for on-line sample        Now there is no requirement to purchase
preparation, column switching or on-line            functions that will not be used. The
microdialysis. The power of the Coulochem III       Coulochem III detector offers flexibility in both
permits fully automated analyses. Methods can       experimental and instrument design, ensuring
be easily recalled for later use. With Time Line    that it will never become obsolete.
Applications
                 SENSITIVITY, RELIABILITY, CONVENIENCE AND FLEXIBILITY ALL
                  COME TOGETHER TO SET A NEW STANDARD IN PERFORMANCE


                                                                                                                        Channel 1 (E1)




                                                                                                                                     8OH2’dG
                                                     Extracted Plasma Sample 20 nA gain
       Basal Frontal Cortical Microdialysis                                                   Channel 2 (E2)
       Perfusate Sample 2 nA gain




                                                                                                      Gua


                                                                                                            7MG
                                                                                                                  2dG
                                                                                                   3MG
                                                                                                  Guo




                                                                                                                              O6MG
                                                               Time (minutes)



 NEUROTRANSMITTERS                            PLASMA CATECHOLAMINES                       OXIDATIVE STRESS MARKERS
 With the wide choice of cells                High selectivity along with                 Using the dual electrode
 available the Coulochem III                  sensitivity can be achieved                 capability of the Coulochem III
 detector can be optimized for                through the dual electrodes                 detector you can “tune” into
 any sample or application. For               of the ESA Coulometric cells.               specific analytes. With the
 microdialysis in neuroscience,               In the analysis of the plasma               guanine adducts you can
 extensive research by the ESA                catecholamines all the                      measure both guanine and the
 Applications Laboratory has                  components are oxidized at                  oxidative adduct simultaneously,
 determined that the ideal                    the first electrode and then                without the vagaries added with
 configuration for sensitivity                measured reductively at the                 multiple detectors.
 and reliability is the Model                 second electrode. In this way
 5041 cell with a microbore                   many common interferences
 column.                                      are eliminated. This is only
                                              possible with the high
                                              efficiency of the coulometric
                                              electrode.


  MAXIMIZING RELIABILITY                       ENHANCED SELECTIVITY                               FLEXIBILITY
     AND SENSITIVITY                             WITH REDOX MODE
         Flavonoids                         Phytoestrogens       Octopamine             Apomorphine           Ergotamine
         Neurotransmitters                  Food/Beverage        Compound               Polyaromatic          Haloperidol
         BHT/BHA                            Catecholamines        NanoSynthesis          Hydrocarbons         Ascorbic Acid
         Pharmaceuticals                    Homocysteine         Monoamines             Lipoic acid           Phenothiazines
         Glutathione                        Antioxidants         Tea Catechins          Xenoestrogens         Acetaminophen
         Metabolism                         Nutraceuticals       Adduct Formation       Hydroxyl Free         Tricyclic
         ROS/RNS                            Herbicides           Serotonin               Radicals              Antidepressants
         Guanine Adducts                    Erythromycin         GABA/Glutamate         Amino Acids           Oxidative Stress
         Carbohydrates                      Morphine             Catecholestrogens      Bisphenol              Markers
         3-Nitrotyrosine                    Phenols              Tocopherol             Capsaicin             Acetylcholine/Choline
         Vitamins                           Antibiotics          Pharmacokinetics       Proanthocyanidins     Thiols




         Thiols, disulfides and thiothers




THIOLS                                               SYNTHESIS                               ASSISTED IONIZATION
Problem analysis, such as the                        The electrochemical redox               React “problem” compounds to
thiol glutathione, is made                           process is related to biolgoical        enable or enhance detectability
reliable with the Boron-Doped                        redox and drug metabolism.              in MS or LC-MS systems.
Diamond (BDD) electrode.                             Synthesize authentic drug               Identify parent compounds
This electrode has a wide                            metabolites in hours, not               and reaction products or
potential window, even with                          months, in quantities suitable          metabolites. Provide additional
aqueous mobile phases, thus                          for NMR, MS or biological               capabilities to complement MS
widening the range of mole-                          studies. Connect on-line with           or LC-MS operations.
cules addressed by ECD. This,                        MS to access metabolic
along with the low fouling and                       products or stability. Go
resilience of the BDD electrode                      off-line to collect metabolites
facilitates the use of ECD for                       in microgram quantities.
many difficult analytes.                             Produce intermediates to
                                                     simplify batch synthesis.



   EXPANDED RANGE OF                                      MORE THAN HPLC                                EC-MS
       ANALYTES
Specifications and Certifications




Detector Configuration:           DC potentiostat for 1 or 2 cells and Potentiostat for Guard Cell and/or Scan Mode/Pulse Mode
Operating Modes:                  DC, Pulse, Scan (Time Line method can be attached to DC and Pulse methods)
Potential Range:                  ± 2000 mV in 1 mV steps
Full Scale Current Range:         10 pA to 1 mA in 1-2-5 sequence (DC Mode)
Filter Time Constants:            0.2 to 10 seconds in 1-2-5 sequence (DC mode)
Typical Noise:                    < 750 fA (0.75 pA) with a 500 MΩ, 0.47 µF test load and a 2 second filter (DC Mode)
Signal Output:                    ± 100 mV, ± 1 V
Output Resolution:                24 bit bipolar
Guard Cell Potential:             ± 2000 mV in 1 mV steps
Event Marker:                     Triggered by front panel keypad, timed operation or RS-232C control
Autozero:                         Triggered by front panel keypad, rear panel contact closures, timed operation or RS-232C control
Function Keys:                    Autozero, Event Marker, Cell On/Off, Run/Stop
RS-232C Interface Capability:     Full parametric instrument control of DC and Pulse
Method Storage:                   Up to 25 methods (any combination of DC, Scan, Pulse and Time Line)
Power:                            100-240 V AC, 50/60 Hz, 24 VA
Detector Dimensions:              19.5” (L) x 9” (W) x 11.25” (H), (49.5 cm x 23 cm x 28.6 cm)
Detector Weight:                  14.8 lbs (6.7 kg) Detector only with DC and Pulse/Scan
Certifications:                   UL, CUL and CE
Other:                            Active input for cell protection


Time Line Specifications (DC and Pulse Mode)
Programmable Changes:             Selectable per channel at times from 0 to 9999.99 minutes in 0.01 minute increments
Autozero, Event Mark:             Independently selectable at start of program and with each current range change
Program Start:                    Via front panel keypad or rear panel contact closure
External Device Control:          Control of up to 5 external devices
Program Control:                  Current Range, Autozero, Event Mark, Potential, Filter Constant
Program Repeats:                  1 to 9999
Automated CV Curve:               Start and Stop Potential, Potential Increment for automating determination of optimal operating potential

About ESA - A Dionex Company
For more than 40 years, ESA Biosciences has been dedicated to developing solutions for bioanalytical detection – a resolve that has resulted
in many US and foreign patents for analytical and diagnostic devices. We are a full-service, ISO-certified and FDA-registered supplier of
instruments, reagents, and turnkey detection systems. Whatever the level of need, you can count on ESA for support – from help in making
the best choice for your application, through installation, training, and post-sale service and support.
For more information, visit: www.esainc.com




                      ESA - A Dionex Corporation                  Corporate Headquarters
                      22 Alpha Road                               Dionex Corporation
                      Chelmsford, MA 01824-4171 USA               1228 Titan Way
                      +1 978.250.7000 Phone                       Sunnyvale, CA 94088-3603
                      +1 978.250.7090 Fax                         +1 408.737.0700 Phone
                      +1 800.959.5095 US Toll free                +1 408.730.9403 Fax
                      Email: info@esainc.com                      www.dionex.com
                      www.esainc.com


                      ESA - A Dionex Company is ISO 9001 and ISO 13485 certified. Specifications subject to
                      change without notice. ESA and Coulochem are registered trademarks of the Dionex
                      Corporation. The Coulochem detector is covered by multiple patents. All other trademarks are
                      property of their respective owners.                                                                                     70-5445
                                                                                                                                               Rev E

								
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