T H E D E M A N D F O R H I G H LY S E N S I T I V E A N D
SELECTIVE DETECTION IN HPLC HAS NEVER BEEN
G R E AT E R . E L E C T R O C H E M I C A L D E T E C T I O N I S
THE ANSWER WHEN YOU NEED TO MEASURE PICOGRAM TO
FEMTOGRAM LEVELS OF OXIDIZABLE
O R R E D U C I B L E C O M P O U N D S.
OF ALL THE HPLC DETECTORS, NONE ARE AS SENSITIVE
AND SELECTIVE WITH AS WIDE A DYNAMIC RANGE AS AN
E L E C T R O C H E M I C A L D E T E C T O R F R O M E S A.
The ESA Coulochem® III detector is the standard in
high sensitivity electrochemical detection. With its modular
design, the Coulochem III platform can be used with any
HPLC system. All new electronics with the latest in digital
technology guarantees the lowest detection limits possible.
Principle of Coulometric Technology:
Compound A is completely reacted at
Sensor 1 thus eliminating it from detection
The Coulochem III incorporates DC and Pulse/Scan modes
at Sensor 2. Compound B passes Sensor 1
unchanged and is detected at Sensor 2.
in discreet modules. By optimizing the detector for the low
The result is that A and B are resolved
signal in DC, the detector achieves the highest possible
sensitivity from capillary to normal bore chromatography.
Pulse mode supports variable timing of potentials and duration
of waveforms to optimize the application. Scan mode helps
select the optimal potentials. The Coulochem III detector
offers DC, Pulse/Scan or both.
“The ESA Coulochem is by far the most
sensitive EC detector on the market today,
very reliable and backed by superior
technical support. Its sensitivity makes
it stand out from the others.”
- Professor Timothy Maher
Massachusetts College of Pharmacy
and Health Sciences
With the sensitivity
and selectivity of the
Coulochem III, peer
into the living brain,
view the mechanisms
of aging and cancer For DC mode, each Coulochem III detector comes with
or create a picture
two analytical channels and an additional channel, unique
of health and disease.
to ESA, for mobile phase cleaning or sample pretreatment.
In Pulse mode, you can select potential and duration of up to
4 segments to give the ultimate in stability and reproducibility.
Operate switching valves or signal for external events, change gain
ranges or potentials with Time Line or shut off cells at the end
of a run. All this stored as one of 25 methods for easy recall.
All standard ESA cell designs can be used with the
Coulochem III detector platform. Expanded gain ranges make it
ready for any cell type. Enhanced processor speed provides data
acquisition fully compatible with today’s faster chromatography.
THE IMPACT OF
RELIABILITY IS ONE
OF THE KEY DESIGN
ELEMENTS OF THE
State-of-the-industry electronics guarantee reliability. The proven
Coulometric cell design is well known for ruggedness and operating every
time right out of the box. The solid state reference electrode used in all ESA
cells never needs replacing, refilling or any other maintenance.
Hands off, fully automated operation is a reality.
MORE TO RELIABILITY THAN JUST HARDWARE.
ESA has been making electrochemical detectors for over 40 years. The
name ESA is synonymous with reliable electrochemical detection in HPLC.
The Coulochem III is backed by superior instrument service and superlative
applications support by experts that specialize in ECD. ESA electrochemical
detectors are used in the most demanding environments imaginable. From
nuclear reactors to clinical laboratories – the Coulometric family of
detectors have proven their reliability.
MODULAR BOARDS easier to read OPTIONAL
a choice of modes: with more INTEGRATED ORGANIZER
DC, Pulse/Scan or useful information protects columns
LARGE KEY PAD
easy to see, both and cells, with or with-
easy to use out temperature control
A CHOICE OF CELLS
current and future ADVANCED ELECTRONICS
menus that are
easy to navigate
The advanced features of the
Coulochem III platform provide
performance and reliability in a
detector that is convenient to use.
The Coulochem III detector was
designed by people that use
ECD every day.
THE ENDLESS POSSIBILITIES OF
A CHOICE OF CELLS FOR OPTIMIZATION OF YOUR ANALYSIS
The Coulochem III detector comes with a
choice of cell designs. From simple analysis
with amperometric electrodes to sophisticated
sample manipulation with Coulometric cells,
ESA offers the flexibility to save time and
effort in optimizing analytical conditions.
All ESA cells come standard with a solid
state, maintenance free reference electrode
known world wide for its reliability.
COULOMETRIC CELLS – There is no PRE-ANALYTICAL CELLS – The high
electrochemical cell more sensitive or reliable. Period. conversion efficiency of the coulometric
Proven in thousands of laboratories worldwide, the electrode when used before the analytical cell
flow-through design with high surface area and provides an additional level of selectivity.
minimized diffusion distance provides 100% analyte When placed before the injector, mobile phase
conversion and the highest signal possible. Each cell can be electrochemically cleaned for lower
has two working electrodes for maximum analytical background and enhanced performance.
flexibility using modes pioneered by ESA’s
Applications Laboratory. Screen and Redox modes SPECIALTY CELLS – Some applications
increase selectivity and reduce sample preparation. have unique requirements. The 5014B and
Channel ratioing gives you added assurance of peak 5041 cells are specifically designed and tested
identity. When downtime is unacceptable, ESA for the demands of microdialysis in
Coulometric cells are the only answer. neuroscience where extreme sensitivity is
required and high sample salt content is
AMPEROMETRIC CELLS – These single encountered.
amperometric electrodes are routinely used for
capillary or microbore chromatography, in pulse SYNTHESIS CELLS – Electrochemical
mode or when metal electrodes are required. This oxidation can be used to produce drug
design is used with the ESA Boron Doped Diamond metabolites in quantities suitable for
electrode which expands the useable potentials range MS, NMR and biological studies.
in electrochemical detection.
THE COULOCHEM III GIVES YOU FLEXIBILITY
IN BOTH EXPERIMENTAL AND INSTRUMENT DESIGN
THAT MEANS YOUR DETECTOR WILL NEVER BECOME OBSOLETE
The Coulochem III detector is a platform for you can program gain changes, run Clean Cell
the future. It is designed so that you can recon- or turn off pumps at the end of a method.
figure the instrument now or in the future to fit
your analytical needs.
DC OR PULSE: YOU CHOOSE
The organizer module houses multiple
columns, Analytical and Guard cells and pulse
dampers. It conveniently mounts a manual
injector. Once inside, cells and
columns are protected from high sensitivity
the environment. The DC and pulse
organizer can be easi- components on the
ly removed or same boards. This
added without means that the
tools. A complete electronics are
analytical config- compromised for the
uration can be differing requirements
of these disparate
modes of operation. In
and stored for a
the Coulochem III there is no
quick and easy
compromise. DC and pulse
change of method. are on separate boards, opti-
mizing the electronics for each
OPERATE EXTERNAL DEVICES mode.
When DC or Pulse mode is needed, simply add
The Coulochem III detector allows operation of
the specific board for a fully optimized detector.
external switching valves for on-line sample Now there is no requirement to purchase
preparation, column switching or on-line functions that will not be used. The
microdialysis. The power of the Coulochem III Coulochem III detector offers flexibility in both
permits fully automated analyses. Methods can experimental and instrument design, ensuring
be easily recalled for later use. With Time Line that it will never become obsolete.
SENSITIVITY, RELIABILITY, CONVENIENCE AND FLEXIBILITY ALL
COME TOGETHER TO SET A NEW STANDARD IN PERFORMANCE
Channel 1 (E1)
Extracted Plasma Sample 20 nA gain
Basal Frontal Cortical Microdialysis Channel 2 (E2)
Perfusate Sample 2 nA gain
NEUROTRANSMITTERS PLASMA CATECHOLAMINES OXIDATIVE STRESS MARKERS
With the wide choice of cells High selectivity along with Using the dual electrode
available the Coulochem III sensitivity can be achieved capability of the Coulochem III
detector can be optimized for through the dual electrodes detector you can “tune” into
any sample or application. For of the ESA Coulometric cells. specific analytes. With the
microdialysis in neuroscience, In the analysis of the plasma guanine adducts you can
extensive research by the ESA catecholamines all the measure both guanine and the
Applications Laboratory has components are oxidized at oxidative adduct simultaneously,
determined that the ideal the first electrode and then without the vagaries added with
configuration for sensitivity measured reductively at the multiple detectors.
and reliability is the Model second electrode. In this way
5041 cell with a microbore many common interferences
column. are eliminated. This is only
possible with the high
efficiency of the coulometric
MAXIMIZING RELIABILITY ENHANCED SELECTIVITY FLEXIBILITY
AND SENSITIVITY WITH REDOX MODE
Flavonoids Phytoestrogens Octopamine Apomorphine Ergotamine
Neurotransmitters Food/Beverage Compound Polyaromatic Haloperidol
BHT/BHA Catecholamines NanoSynthesis Hydrocarbons Ascorbic Acid
Pharmaceuticals Homocysteine Monoamines Lipoic acid Phenothiazines
Glutathione Antioxidants Tea Catechins Xenoestrogens Acetaminophen
Metabolism Nutraceuticals Adduct Formation Hydroxyl Free Tricyclic
ROS/RNS Herbicides Serotonin Radicals Antidepressants
Guanine Adducts Erythromycin GABA/Glutamate Amino Acids Oxidative Stress
Carbohydrates Morphine Catecholestrogens Bisphenol Markers
3-Nitrotyrosine Phenols Tocopherol Capsaicin Acetylcholine/Choline
Vitamins Antibiotics Pharmacokinetics Proanthocyanidins Thiols
Thiols, disulfides and thiothers
THIOLS SYNTHESIS ASSISTED IONIZATION
Problem analysis, such as the The electrochemical redox React “problem” compounds to
thiol glutathione, is made process is related to biolgoical enable or enhance detectability
reliable with the Boron-Doped redox and drug metabolism. in MS or LC-MS systems.
Diamond (BDD) electrode. Synthesize authentic drug Identify parent compounds
This electrode has a wide metabolites in hours, not and reaction products or
potential window, even with months, in quantities suitable metabolites. Provide additional
aqueous mobile phases, thus for NMR, MS or biological capabilities to complement MS
widening the range of mole- studies. Connect on-line with or LC-MS operations.
cules addressed by ECD. This, MS to access metabolic
along with the low fouling and products or stability. Go
resilience of the BDD electrode off-line to collect metabolites
facilitates the use of ECD for in microgram quantities.
many difficult analytes. Produce intermediates to
simplify batch synthesis.
EXPANDED RANGE OF MORE THAN HPLC EC-MS
Specifications and Certifications
Detector Configuration: DC potentiostat for 1 or 2 cells and Potentiostat for Guard Cell and/or Scan Mode/Pulse Mode
Operating Modes: DC, Pulse, Scan (Time Line method can be attached to DC and Pulse methods)
Potential Range: ± 2000 mV in 1 mV steps
Full Scale Current Range: 10 pA to 1 mA in 1-2-5 sequence (DC Mode)
Filter Time Constants: 0.2 to 10 seconds in 1-2-5 sequence (DC mode)
Typical Noise: < 750 fA (0.75 pA) with a 500 MΩ, 0.47 µF test load and a 2 second filter (DC Mode)
Signal Output: ± 100 mV, ± 1 V
Output Resolution: 24 bit bipolar
Guard Cell Potential: ± 2000 mV in 1 mV steps
Event Marker: Triggered by front panel keypad, timed operation or RS-232C control
Autozero: Triggered by front panel keypad, rear panel contact closures, timed operation or RS-232C control
Function Keys: Autozero, Event Marker, Cell On/Off, Run/Stop
RS-232C Interface Capability: Full parametric instrument control of DC and Pulse
Method Storage: Up to 25 methods (any combination of DC, Scan, Pulse and Time Line)
Power: 100-240 V AC, 50/60 Hz, 24 VA
Detector Dimensions: 19.5” (L) x 9” (W) x 11.25” (H), (49.5 cm x 23 cm x 28.6 cm)
Detector Weight: 14.8 lbs (6.7 kg) Detector only with DC and Pulse/Scan
Certifications: UL, CUL and CE
Other: Active input for cell protection
Time Line Specifications (DC and Pulse Mode)
Programmable Changes: Selectable per channel at times from 0 to 9999.99 minutes in 0.01 minute increments
Autozero, Event Mark: Independently selectable at start of program and with each current range change
Program Start: Via front panel keypad or rear panel contact closure
External Device Control: Control of up to 5 external devices
Program Control: Current Range, Autozero, Event Mark, Potential, Filter Constant
Program Repeats: 1 to 9999
Automated CV Curve: Start and Stop Potential, Potential Increment for automating determination of optimal operating potential
About ESA - A Dionex Company
For more than 40 years, ESA Biosciences has been dedicated to developing solutions for bioanalytical detection – a resolve that has resulted
in many US and foreign patents for analytical and diagnostic devices. We are a full-service, ISO-certified and FDA-registered supplier of
instruments, reagents, and turnkey detection systems. Whatever the level of need, you can count on ESA for support – from help in making
the best choice for your application, through installation, training, and post-sale service and support.
For more information, visit: www.esainc.com
ESA - A Dionex Corporation Corporate Headquarters
22 Alpha Road Dionex Corporation
Chelmsford, MA 01824-4171 USA 1228 Titan Way
+1 978.250.7000 Phone Sunnyvale, CA 94088-3603
+1 978.250.7090 Fax +1 408.737.0700 Phone
+1 800.959.5095 US Toll free +1 408.730.9403 Fax
Email: firstname.lastname@example.org www.dionex.com
ESA - A Dionex Company is ISO 9001 and ISO 13485 certified. Specifications subject to
change without notice. ESA and Coulochem are registered trademarks of the Dionex
Corporation. The Coulochem detector is covered by multiple patents. All other trademarks are
property of their respective owners. 70-5445