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ADVIA CENTAUR

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					 eVAluAtIon oF the bAyer AdVIA centAur cP ImmunoAssAy AnAlyser
                ¹C-F Lim, ¹D Mitchell, ¹A McNeil, ¹S Neate, ²P Maguire, ²J Fernandez ¹Symbion Health Dorevitch Pathology, Heidelberg, VIC, 3084 and ²Bayer HealthCare, Scoresby, VIC, 3179.

IntroductIon:                                                results:                                                                                                                                                                               tAble 1:

The Bayer ADVIA Centaur CP analyser is an automated          1.	The	ADVIA	Centaur	CP	generates	its	first	test	result	15	minutes	after	                                                                                                                Summary data for the correlation analysis of Troponin I,
bench top immunoassay analyser recently released in             sampling	the	first	specimen.	The	maximum	throughput	is	180	tests/                                                                                                                     Oestradiol, Follicle Stimulating Hormone, Human Chorionic
Australia. This analyser is intended to replace the widely      hour.                                                                                                                                                                                 Gonadotrophin, Leutinising Hormone and Progesterone.
used ACS:180 analyser. ADVIA Centaur CP shares the                                                                                                                                                                                                    Each analyte was evaluated using at least 75 patient
same reagents with ADVIA Centaur.                                                                                                                                                                                                                     samples.
                                                             2. Figures 1, 2 and 3 depict the typical correlation between ADVIA
We evaluated the performance of ADVIA Centaur                   Centaur CP with ADVIA Centaur and ACS:180, respectively. The
CP against two other Bayer immunoassay analysers                correlation data of the six analytes is summarised in Table 1.
                                                                                                                                                                                                                                                                        Centaur CP vs Centaur                Centaur CP vs ACS:180
currently in use in Symbion Health, namely ADVIA
Centaur and ACS:180.                                                                                                                                                                                                                                                   Slope    Intercept        R²           Slope                      R²
                                                             3. Table 2 shows the between run imprecision data using commercial                                                                                                                            CTNI        1.22          -0.06      0.987         1.18          0.13    0.987
                                                                quality control materials.                                                                                                                                                                  *E2        1.09          31.90      0.973         1.36          18.27   0.942
AIms:                                                                                                                                                                                                                                                      FSH         1.05          0.45       0.992         1.05          0.74    0.989
                                                             FIgure 1: Fsh
To examine (i) the assay correlation of ADVIA Centaur CP                                                                                                                                                                                                   HCG         0.93          9.20       0.994         0.90          3.86    0.986
with the performance of ADVIA Centaur and ACS:180,       Correlation analysis of ADVIA Centaur CP with (A) ADVIA Centaur and                                                                                                                                LH         1.02          0.44       0.995         0.96          -0.12   0.993
respectively, (ii) between assay imprecision, (iii) the  (B) ACS:180                                                                                                                                                                                       Prog        0.97          2.12       0.993         0.87          4.48    0.970
productivity of the analyser.
                                                                                                                                                                                                                                                      *E2-6III was used on ADVIA Centaur CP and ADVIA
                                                                                                   (A) FSH: Centaur CP vs Centaur                                                              (B) FSH: Centaur CP vs ACS:180
                                                                                                                                                                                                                                                      Centaur; E2-6 was used on ACS:180
methods:                                                                      200
                                                                                                 y = 1.0459x + 0.4517
                                                                                                                                                                             200

                                                                                                                                                                                            y = 1.0507x + 0.7399
                                                                                                      R2 = 0.9919                                                                                R2 = 0.9888
                                                                              150                                                                                            150
                                                                                                                                                                                                                                                    tAble 2:
Six representative analytes were chosen for the analysis




                                                                                                                                                              Centaur CP
                                                                 Centaur CP




– Oestradiol (E2), Follicle Stimulating Hormone (FSH),                        100                                                                                            100

                                                                                                                                                                                                                                                      Between run imprecision using either BioRad Cardiac
Human Chorionic Gonadotrophin (HCG), Leutinising                                  50                                                                                           50                                                                     Marker (Level 1 & 2) or Bayer Ligand Plus Quality Control
Hormone (LH), Progesterone (Prog) and Cardiac                                                                                                                                                                                                         (Tri-Level).	The	coefficient	of	variation	ranged	from	0.2%	to	
Troponin I (cTNI).                                                                   0
                                                                                         0                  50                100           150         200
                                                                                                                                                                               0
                                                                                                                                                                                    0                     50              100         150     200     8.0%.
                                                                                                                          Centaur                                                                                       ACS:180


Patient samples for routine analysis were used for the
study. The correlation analysis of each analyte was                                                                                                                                                                                                          Analyte      Quality        Mean     Std Dev            CV %           N
performed using at least 75 patient samples. These                                                                                                                                                                                                                        Control

samples were selected based on their respective              FIgure 2: hcg                                                                                                                                                                                     cTNI     BioRad L1
                                                                                                                                                                                                                                                                               L2
                                                                                                                                                                                                                                                                                         0.37
                                                                                                                                                                                                                                                                                         8.45
                                                                                                                                                                                                                                                                                                      0.027
                                                                                                                                                                                                                                                                                                      0.305
                                                                                                                                                                                                                                                                                                                      7.3
                                                                                                                                                                                                                                                                                                                      3.6
                                                                                                                                                                                                                                                                                                                                    26

concentrations on the ADVIA Centaur analyser.                                                                                                                                                                                                                    E2     Bayer   L1        463          25             5.3           24
                                                             Correlation analysis of ADVIA Centaur CP with (A) ADVIA Centaur and                                                                                                                                                L2        907          47             5.2
                                                                                                                                                                                                                                                                                L3       1946         156             8.0
The between run imprecision (n = 16 - 65) was evaluated      (B) ACS:180
                                                                                                                                                                                                                                                               FSH      Bayer   L1       64.6          0.8            1.2           26
using commercial quality control materials, namely                                                                                                                                                                                                                              L2       24.2          1.2            5.0
BioRad Cardiac Marker Level 1 & 2 and Bayer Ligand                                                                                                                                                                                                                              L3        4.1         0.25            5.7
                                                                                                                                                                                                                                                               HCG      Bayer   L1        6.5         0.07            1.2           65
Plus Controls (Tri-Level).                                                                          (A) HCG: Centaur CP vs centaur                                                            (B) HCG: Centaur CP vs ACS:180                                                    L2       15.9          0.6            3.8
                                                                                                                                                                                                                                                                                L3       452           23             5.1
                                                                                  1200                                                                                       1200
                                                                                                                                                                                                                                                                 LH     Bayer   L1        4.6         0.11            2.4           16
The productivity of ADVIA Centaur CP was evaluated by                                              y = 0.9261x + 9.2018
                                                                                                        R2 = 0.9942
                                                                                                                                                                                            y = 0.897x + 3.8607
                                                                                                                                                                                                R2 = 0.9864                                                                     L2       17.3          0.7            4.0
the number of tests processed per hour.                                              900                                                                                       900                                                                                              L3        34          0.07            0.2
                                                                                                                                                                Centaur CP
                                                                     Centaur CP




                                                                                                                                                                                                                                                               Prog     Bayer   L1       1.48         6.95            6.1           24
                                                                                     600                                                                                       600                                                                                              L2       7.47         0.32            4.3
                                                                                                                                                                                                                                                                                L3       23.0         0.20            0.9


AdVIA centAur cP
                                                                                                                                                                               300
                   ®                                                                 300




                                                                                         0                                                                                          0                                                               summAry And conclusIons:
                                                                                             0                  300            600          900        1200                             0                 300             600        900    1200

                                                                                                                             Centaur                                                                                    ACS:180

                                                                                                                                                                                                                                                    1. There was good agreement between ADVIA Centaur
                                                                                                                                                                                                                                                       CP and ADVIA Centaur for oestradiol, follicle stimulating
                                                                                                                                                                                                                                                       hormone, human chorionic gonadotrophin, leutinising
                                                                                                                                                                                                                                                       hormone and progesterone. A positive bias was observed
                                                             FIgure 3: ctnI                                                                                                                                                                            for troponin I. For ACS:180, good agreement was observed
                                                                                                                                                                                                                                                       for FSH and LH, while a positive bias (cTNI & E2) and a
                                                             Correlation analysis of ADVIA Centaur CP with (A) ADVIA Centaur and
                                                                                                                                                                                                                                                       negative bias (HCG & Prog) were observed.
                                                             (B) ACS:180
                                                                                                                                                                                                                                                    2. The time taken for ADVIA Centaur CP and ADVIA Centaur
                                                                                                     (A) TNI: Centaur CP vs Centaur                                                             (B) TNI: Centaur CP vs ACS:180
                                                                                                                                                                                                                                                       to	generate	their	first	test	result	is	15	and	18	minutes,	
                                                                                     50
                                                                                                 y = 1.2181x - 0.0607
                                                                                                                                                                               50
                                                                                                                                                                                            y = 1.1764x + 0.1265
                                                                                                                                                                                                                                                       respectively.
                                                                                                       2                                                                                         2
                                                                                                     R = 0.9868                                                                                 R = 0.9873
                                                                                     40                                                                                        40


                                                                                                                                                                                                                                                    3. The maximum throughput for ADVIA Centaur CP is 180
                                                                                                                                                                  Centaur CP
                                                                        Centaur CP




                                                                                     30                                                                                        30

                                                                                                                                                                                                                                                       tests/hour	while	the	maximum	throughput	for	ADVIA	
                                                                                                                                                                                                                                                       Centaur	is	240	tests/hour.	
                                                                                     20                                                                                        20


                                                                                     10                                                                                        10


                                                                                         0                                                                                      0                                                                   4. The quick turn-around-time together with the high
                                                                                                                                                                                                                                                       throughput means that the ADVIA Centaur CP is well suited
                                                                                             0             10           20             30         40    50                              0            10            20           30     40    50

                                                                                                                          Centaur                                                                                   ACS:180
                                                                                                                                                                                                                                                       to the needs of a small to medium sized laboratory.

				
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