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the Clinical Chemist

17th National Meeting
American Association of Clinical Chemists
Sheraton-Chicago Hotel, Chicago, Ill.
Aug. 30-Sept. 2, 1965

Scientific Program
The symposia and invited scientific papers will be presented in the morning ses-
sions. This part of the program has appeared in the June issue of CLINICAL
CHEMISTRY.

Program of Contributed Papers
Monday, Aug. 30 (P.M.)
Session I-Enzymes (East Room, 9th floor)
Chairman, MORTON K. SCHWARTZ (Memorial Hospital, New York, N. Y.).
1:30 (1) An ultramicromethod for serum amylase deterlnination. RONALD Ti.
SEARCY, SHINICHIRO HAYASHI, and J. EDWARD BERK (California College
of Medicine and Los Angeles County General Hospital (Unit 2), Los
Angeles, Calif.).
1 :50 (2) An inhibitor of serum amylase: The problems posed by methodology.
SHINICHIRO HAYASHI, RONALD L. and J. EDWARD BERK (Cali-
SEARCY,
fornia College of Medicine and Los Angeles County General hospital
(Unit 2), Los Angeles, Calif.).
2:10 (3) Tryptic and chymotryptic activity of stools as a diagnostic tool in
the pancreatic insufficiency of cystic fibrosis. GIULO J. BARBERO, .JEAN M.
MARINO, RONALD SEIBEL, and MAARTEN S. SIBINGA (Children’s Hospital of
Philadelphia, Philadelphia, Pa.).
2 :30 (4) Tryptie-like esterase activity of normal human serum and plasma.
R. B. RUTKOWSKI (Walter Reed Army Institute of Research, Washington,
D. C.).
2 :50 Coffee break

3:20 (5) Trypsin stabilizers in human serum-the role of aa-macroglobulin.
K. JAMES, F. B. TAYLOR, JR., and H. H. FUDENBERG (University of Cali-
fornia, San Francisco Medical Center, San Francisco, Calif.).
3:40 (6) Phenolphthalein monophosphate, a new substrate for alkaline phos-
733
734 THE CLINICAL CHEMIST Clinical Chemistry

phatase. ARTHUR L. BABSON (The Warner-Lambert Research Institute,
Morris Plains, N. J.).
4:00 (7) Heat inactivation in the study of human alkaline phosphatases.
S. POSEN, F. C. NEALE, and J. S. CLUBB (Sydney Hospital, Sydney,
Australia).
4:20 (8) Lactic dehydrogenase in urine. ARTHUR L. LEVY and DONALD R.
COLE (St. Vincent’s Hospital, New York, N. Y.).
4:40 (9) A colorimetric lactic acid dehydrogenase method. GEORGE W. CHAP-
MAN and JOSEPH H. BOUTWELL, JR. (Temple University Hospital, Phila-
delphia, Pa.).

Session Il-General Methods (Tally-Ho Room, 9th floor)
Chairman, BERNARD KLEIN (Veterans Administration Hospital, Bronx,
N.Y.).
1:30 (10) Studies on the linearity of the Jaffe reaction for the determination
of high creatinine concentrations in serum and urine. D. A. ARyAN, H. M.
RAWNSLEY, and A. E. ENGSTROM (University of Pennsylvania, Philadel-
phia, Pa.).
1 :50 (11) Creatine in blood serum: Its deterlnination with creatine phospho-
kinase. ANDRE C. KIBRICK and ADE T. MILHORAT (Institute for Muscle
Disease, Inc., New York, N. Y.).
2:10 (12) Application of a glucose-oxidase method to serum. ARNOLD G. WARE
and EDWARD P. MARBACII (Los Angeles Count.y General Hospital, Los
Angeles, Calif.).
2:30 (13) Automatic micro glucose determination with a 60-second direct-
reading analyzer. F. LIM (Medical College of Virginia, Richmond, Va.).

2:50 Coffee break

3:20 (14) Separation and quantitation of human hemoglobins on cellulose
acetate using a continuous buffer system. ,JENNIE D. SEATON (University
of Kentucky, Lexington, Ky.).
3 :40 (15) A comparison of bilirubin determinations by automated and hand
methods. JOHN DAVISON and GEORGE TURNER (University of Virginia
Hospital, Charlottesville, Va.).
4:00 (16) The determination of serum iron by atomic absorption spectro-
photometry. DENIS 0. RODGERSON and RAY E. HELFER (University of
Colorado Medical Center, Denver, Cob.).
4:20 (17) Equivalent volume concept: A promising technic in analytical
chemistry. 1. Uric acid. ,JONAS MAURUKAS (Elyria Memorial Hospital,
Elyria, Ohio).
4:40 (18) Evaluation of automated flame photometry using natural gas/02
versus propane/02 with specimens from the same dialysate. ALFRED B.
MILLER, ELBERT HICKS, CURT M. JENSEN, and HAROLD D. APPLETON (Met-
ropolitan Hospital and New York Medical College, New York, N. Y.).
Vol. II, No. 7, 1965 THE CLINICAL CHEMIST 735

Tuesday,Aug. 31 (P.M.)
Session Ill-Enzymes (East Room, 9th floor)
Chairman, FRANK A. IBB0’rT (University of Colorado Medical Center,
Denver, Cob.).
1 :30 (19) Correlation of urine and serum enzyme activities in myocardial
infarction. ALBERT A. DIETZ, LAVERNE K. HOnOES, and DONALD Fox-
WORTHY (Veterans Administration Hospital, Hines, Ill.).
1 :50 (20) The separation of a-hydroxybutyric dehydrogenase, lactic dehy-
drogenase, and creatine phosphokinase isoenzymes using Sephadex-
DEAE. R. P. MACDONALD, J. W. HESS, and G. J. NATHO (Harper Hos-
pital and Wayne State University School of Medicine, Detroit, Mich.).
2 :10 (21) Enzyme fractionation by means of continuous-flow electrophoresis.
SEYMOUR WINSTEN, JEAN JACKSON, and PAULA WOLFE (Albert Einstein
Medical Center, Philadelphia, Pa.).
2 :30 (22) Investigation of ribonuclease isOenzymes by an electrophoretic-
ultraviolet method. NEWTON RESSLER, E. OLIvERO, G. R. THOMPSON, and
R. R. JOSEPH (Wayne County General Hospital, Eloise, Mich.).

2:50 Coffee break
3:20 (23) Fluorometric determination of creatine kinase activity. SYLVAN M.
SAX and JOHN J. MOORE (The Western Pennsylvania Flospital, Pittsburgh,
Pa.).
3:40 (24) Cholinesterase profiles of certain mammalian sera. CHARLES E.
BECKER (School of Dentistry, University of Detroit, Detroit, Mich.).
4 :15 Ames Award Lecture (East Room, 9th floor)
Chairman, 0. H. GAEBLER (Edsel B. Ford Institute for Medical Research,
Detroit, Mich.).
Session
I V-Proteins (Tally-Ho Room, 9th floor)
Chairman, ABRAHAM SAIFER (Jewish Sanatorium and hospital for Chronic
Diseases, Brooklyn, N. Y.).
1 :30 (25) Serum protein fractions. BASIL DOUMAS and D. B. MORRISON (Uni-
versity of Alabama Medical College, Birmingham, Ala., and University of
Tennessee, Memphis, Tenn.).
1 :50 (26) Isolation of albumin from human serum by means of trichloroaeetic
acid and ethanol. TA.xuzo IwATA, Hm0MI IWATA, and JAMES F. hOLLAND
(Roswell Park Memorial Institute, Buffalo, N. Y.).
2:10 (27) Comparison of salting-out technics for plasma fibrinogen with a
micro-sulfite fractionation technic. J. F. GOODWIN and B. MURPHY (Chil-
dren’s Hospital of Michigan and Wayne State University School of
Medicine, Detroit, Mich.).
2:30 (28) Pyroglobulins in multiple myeloma. H. M. RAWNSLEY, II. M.
BOWMAN, and D. ARYAN (University of Pennsylvania, Philadelphia, Pa.).
2:50 Coffee break
736 THE CLINICAL CHEMIST Clinical Chemistry

3 :20 (29) Concentration of cerebrospinal fluid proteins and their fractiona-
tion by cellulose acetate electrophoresis. ALEX KAPLAN and MURRAY
JOHNSTONE (University of Washington, Seattle, Wash.).
3 :40 (30) Comparison of the effects of tolbutamide and phenethylbiguanide
on hepatic protein synthesis. huGH J. MCDONALD and LAWRENCE R.
DECHATELET (Loyola University, Stritch School of Medicine, Chicago,
Ill.).
4:15 Ames Award Lecture (See under Session III)

Wednesday, Sept. 1 (P.M.)

Session V--Automation. (East. Room, 9th floor)
Chairman, WILLARD R. FAULKNER (Cleveland Clinic Foundation, Cleve-
land, Ohio).
1 :30 (31) The automated determination of NAD-coupled enzymes. STANLEY

MORGENSTERN, RICHARD FLOR, GERALD KESSLER, and BERNARD KLEIN
(Veterans Administration hospital and Montefiore Hospital, Bronx,
N. Y.)
1 :50 (32) Automated determination of serum glutamie oxalacetie transami-
nase. STANLEY MORGENSTERN, MORRIS OKLANDER, JOSEPH AUERBACH,
JAMES KAUFMAN, and BERNARD KLEIN (Veterans Administration Hos-
pital, Bronx, N. Y.).
2:10 (33) Automated fluorometric determinations of serum glutamic oxala.
cetic transaminase and glutamic pyruvic transaminase. JACOB B. LEVINE
and JOHN B. HILL (Technicon Instruments Corp., Chauncey, N. Y., and
University of North Carolina School of Medicine, Chapel Hill, N. C.).
2:30 (34) A new automated method for the determination of cholinesterase
activity. CHARLES G. HUMISTON and GEORGE J. WRIGHT (The Dow Chem-
ical Company, Midland, Mich.).
2:50 (35) Automated methods for blood glucose and urea. BENJAMIN FINGER-
HUT, Rocco FERZOLA, WALTON H. MARSH, and ALFRED B. MILLER (Kings
County hospital Center, Brooklyn, N. Y.).
3:10 Coffee break
3 :45 Warner-Chilcott Lecture (See program of invited papers.)
Session UI-Metals and Toxicology (Tally-Ho Room, #{244}h floor)
Chairman, FERRLN B. MORELAND (Baylor University College of Medicine,
Houston, Tex.).
1 :30 (36) Determination of copper and zinc in biological material by atomic
absorption spectrophotonetry. MARY M. PARKER and F. L. HUMOLLER
(Veterans Administration Hospital, Omaha, Neb.).
1 :50 (37) Direct photometric analysis of serum calcium with glyoxal bis- (2.
hydroxyanil). MILTON MAGER and GENEVIEVE FARESE (U. S. Army Re-
search Institute of Environmental Medicine, Natick, Mass.).
Vol. II. No. 7, 1965 THE CLINICAL CHEMIST 737

2:10 (38) Hydroxylamine stabilized dyes, related to Calcon, as indicators for
the EDTA titration of calcium in native serum. E. MELVIN GINDLER and
RAYMOND E. VANDERLINDE (Saint Francis General Hospital, Pittsburgh,
Pa., and The Memorial Hospital, Cumberland, Md.).
2 :30 (39) Routine assay of ethanol, methanol, acetone, and isopropariol in
body fluids with the gas chromatograph. SAMUEL NATELSON and RODNEY
L. STELLATE (Michael Reese Hospital, Chicago, Ill.).
2 :50 (40) An improved method for the determination of ethanol in blood by
gas chromatography. CECIL ENTENMAN, JUNE JAEGER, and RICHARD A.
SKAHEN (Institute for Lipid Research, Berkeley, Calif.).
3 :10 Coffee break

Thursday, Sept. 2 (A.M.)
Session Vu-General (East Room, 9th floor)
Chairman, GERARD F. LANCHANTIN (Cedars of Lebanon Hospital, Los
Angeles, Calif.).
9 :00 (41) A precision spectrOphOtolneter f. clifli(al chemistry. S.uiR.
GILFORD, H.. EMARY, and MELVIN MARTENS (Gilford Instrument Labora-
tories, Oberlin, Ohio).
9 :20 (42) Preliminary applications of a new “linear absorbance” spcctropho-
tometer to clinical methods. S. MEITES (The Children’s hospital, Colum-
bus, Ohio).
9:40 (43) Studies on wave length plus photometric accuracy of spectropho-
tometers utilized in the clinical chemistry laboratory. GEORGE N. BOWERS,
JR and ROBERT B. McCoIIB (Hartford Hospital, Ilartford, Conn.).
10 :00 (44) A machine data processing system for clinical chemistry. H. G.
BIGGS (University of Alabama Medical College, Birmingham, Ala.).
10:20 Coffee break
10:40 (45) Nephrotoxicity incident to abdominal aortography with special
reference to proximaltubular function. RACHEL K. YOUNGER, ,JOHN 11.
FOSTER, and HERBERT E. WOLLOWICK (Vanderbilt University Medical
Center, Nashville, Tenn.).
11 :00 (46) A microenzymatic method employing stable reagents for the deter-
mination of ceruloplasmin in serum or blood. JAMES A. SHEI’IIERD (Uni-
versity of Iowa, Iowa City, Iowa).
11 :20 (47) A semiautomated fluorometric technic for serum triglycerides.
GERALD KESSLER and HILDA LEDERER (Montefiore hospital, New York,
N. Y., and Bio-Science Laboratories, Los Angeles, Calif.).
11 :40 (48) Biochemical diagnostic technic use of paper chromatography.
CAROLYN SCHEEL LEONARD and HELEN K. BERRY (Cleveland Metropolitan
General Hospital, Cleveland, Ohio, and Children’s Hospital Research
Foundation, Cincinnati, Ohio).
738 THE CLINICAL CHEMIST Clinical Chemistry

Session Vill-Hormones and General (Tally-Ho Room, 9th floor)
Chairman, RODERICK P. MACDONALD (Harper Hospital, Detroit, Mich.).
9 :00 (49) Fractionation of urinary 17-ketosteroids by gas liquid chromatog-
raphy. CHARLES R. BERRETT and CRICHTON MCNEIL (Holy Cross Hospital
Research Foundation, Salt Lake City, Utah).
9:20 (50) A study on an improved 17-ketosteroid color reaction. LOUIS A.
KRAUSHAAR, EMANUEL EPSTEIN, and BENNIE ZAK (Beaumont Hospital,
Royal Oak, Mich., St. Joseph Mercy Hospital, Pontiac, Mich., and Wayne
State University School of Medicine, Detroit, Mich.).
9:40 (51) Determination of vanilmandelic acid in urine by anion exchange
analysis. GORDON C. MILLS (University of Texas Medical Branch, Galves-
ton, Tex.).
10:00 (52) High voltage electrophoresis for rapid routine screening for pheo-
chromocytoma and functional neuroblastoma. STEFAN EPSTEIN, HENRY
SCHRIEVER, and RAYMOND GAMBINO (Englewood Hospital, Englewood,
N.J.).
10:20 Coffee break

10:40 (53) Conversion of p-hydroxyphenolic acids to aldehydes. BERNADETTE
B. BOURNE and BRUCE MAJILTON (University of Miami, School of Medi-
cine and Edison High School, Miami, Fla.).
11 :00 (54) Calcium homeostasis in the parathyroidect.oniized (log. Ro L.
ALEXANDER, Ja. (Ihillcrest Medical Center, Tulsa, Okla.).
11 :20 (55) Sphingolipids of the brain and spinal cord in experimental “aller-
gic” encephalomyelitis. HENRY P. SCHWARZ, IRMA KOSTYK, and ALFONSO
MARMOLEJO (Philadelphia General Hospital, Philadelphia, Pa.).
11 :40 (56) Nature of the yellow pigment in amniotic fluid of mothers with Rh
sensitization. CHARLES E. WILLIS and WILLARD R. FAULKNER (Cleveland
Clinic, Cleveland, Ohio).

American Microchemical Society Commission Tech-
on Microcliemical
Organizes International Symposium niques, Division Chemis-
of Analytical
try, and is being supported (in part) by
on Microchemical Technics-1965 a research grant from the National In-
stitutes of health, U. S. Public health
The International Symposium on Service.
Microchemical Technics-1965 will be The symposium will provide for an
conducted Aug. 22-27, 1965, by The interchange of information and ideas
Pennsylvania State University through among technologists from all parts of
its College of Science and Continuing the world concerning new methods and
Education. It is being organized by the technics or unique applications in the
American Mierochemical Society with field of microchemistry. The symposium
the sponsorship of the International will take place in the informal atmos-
Union of Pure and Applied Chemistry, phere of fellowship and individual con-
Vol. II, No. 7, 1965 THE CLINICAL CHEMIST 739

tact niade possible by the excellent on- It is anticipated that the program will
campus accommodations and facilities center on the following major themes:
of The Pennsylvania State University. 1. Clinical Chemistry of Proteins
Plenary lectures, original papers, (Except Enzymes)
seminars, a commercial exhibition, tech- 2. Clinical Enzymology
nical films, timely social events, includ-
ing an informal reception and buffet 3. Clinical Chemical Function Tests
dinner, barbecue, and ladies’ events will for the Thyroid and Adrenal Glands
comprise the program. Seventeen tech- 4. Progress of Basic Research in
nical sessions are planned. Of particu- Clinical Chemistry
lar interest to AACC members will be A circular letter with further infor-
the sessions on instrumentation, micro- mation about the congress will be dis-
chemistry, and microchemical methods. tributed shortly.
The location for this international
meeting was selected by the 1965 Sym-
posium Committee of the American Charter Flight to the International
Microchemical Society. Adequate lec- Congress, Munich, Germany-
ture halls, conference and exhibit rooms, 1966
excellent dining and social facilities,
and modern residence halls-all within
Plans are now being formulated for
easy walking distance of each other-
an AACC charter flight for members
were factors influencing this decision.
and their immediate families who wish
Participants and all activities (lectures,
to attend the VI International Congress
meals, and commercial exhibits) except
for Clinical Chemistry. The flight will
the reception and buffet dinner will be
leave from New York City, approxi-
housed in the East Halls Housing Com-
mately July 15, 1966, and will land at
plex.
Amsterdam. This will permit members
For further information, contact
to travel briefly in Europe before the
Howard J. Francis, Jr., Pennsatt Chem-
congress. Several planned tours will be
icals Corp., 900 First Ave., King of
arranged for those who do not wish to
Prussia, Pa., U.S.A., or International
travel individually.
Symposium on Mierochemical Tech-
niques, Conference Center, The Penn- The return flight will leave from Mu-
sylvania State University, University nich and land at New York in sufficient
Park, Pa. 16802. time to permit attendance at the AACC
Annual Meeting in Miami, Aug. 14-21,
1966.
VI International Congress for Special group flights from Chicago
Clinical Chemistry and other points may be arranged to
connect with the charter flight, so that
The VI International Congress for the 5% federal air travel tax may be
Clinical Chemistry will be held in Mu- avoided.
nich, July 26 to July 30, 1966. The con- Members who have already indicated
gress will be organized by the German an interest in the charter flight will
Society for Clinical Chemistry, and soon be contacted directly. Others may
Prof. med. 0. Wieland, who is vice- obtain information by writing directly
president of the society, has been named to Charter Organizer, International
as general secretary for the forthcoming Congress Flight, P.O. Box 109, Cam-
congress. bridge 38, Mass. 02138
740 THE CLINICAL CHEMIST Clinical Chemistry

Section News Drs. C. J. Porter, President of the
Canadian Society, and Diana Michener
Cleveland Secflon Schatz, Secretary of the Canadian So-
The Cleveland Section of the AACC ciety, were chairmen of the scientific
met in joint meeting with the Cleveland sessions which included the following
Society of Pathologists and the Cleve- presentations:
land Society of Medical Technologists “A.B.C.’s of a Coniputor.’ H. Mac-
on Wednesday evening, Mar. 17, 1965, Intyre, Rochester, N. Y.
at the Metropolitan General Hospital of “A Punched Card Systeni for the
Cleveland. The Cleveland Society of Laboratory,” N. Radin, Rochester,
Pathologists was host at a reception and N.Y.
dinner that preceded the scientific pro- “Laboratory Information System
gram. More than 90 members from these -Data processing for tile clinical
societies attended. laboratory that starts with the
Dr. Jerome Berner of the Society of requisition form and acid resses it-
Pathologists opened the meeting. Dr. self to the paper flow into, within,
Irving Sunshine, Chairman of the and out of the laboratory up to the
Cleveland Section AACC, and Mrs. posting of the tests results in the
Barbara Lilly, president of the Cleve- patients’ chart,” W. .J. (‘oiistandse,
land Society of Medical Techimologists, New York, N. Y.
both commented upon the special inter- “Laboratory Data Acquisition Sys-
est in this meeting of the three societies, tem-The elimination of clerical
the first of its kind to be held in Cleve- work performed by medical labora-
land. tory technologists in automated
The scientific program had been ar- tests by hooking up instruments,
ranged and was given by members of such as automatic analyzers, cell
the Cleveland Section of the AACC. counters and photometers to a com-
Drs. J. R. Leonards. R. W. Marsters, puter system,” W. J. Constandse,
J. Waide Price, and Irving Sunshine New York, N. Y.
spoke on kit tests for glucose, amylase, “Cumulative Reports of Labora-
urea, and toxicologic procedures. These tory Results-Description of a sys-
papers stimulated much discussion. Tile tem employed in the Department
success of this meeting was responsible of Biochemistry, University of
for much interest, in the possibility of Rochester School of Medicine and
other joint programs and meetings in Dentistry,” W. B. Mason, llohes-
the future. t.er, N. Y.
“Data Processing of hospital Rec-
Upstate New York Section ords-A description of tile system
A joint 2-day meeting of the Upstate employed by the Ontario hospital
New York Section of the AACC and the Medical Records Tnstitute.’ Miss
Toronto Group of the Canadian Society M. Wilson, Toronto, Ont., Canada.
of Clinical Chemists was held in the “Post - M o r t e in Electrophoresis
Main Lecture Theater of the Toronto Studies with the Aid of a Com-
General Hospital, Toronto, Canada, on puter,” N. Radin, Rochester. N. Y.
May 14-15, 1965. “Unsolicited Laboratory Inforlna-
The presented papers were built tion-Preliminary efforts to study
around the theme of the meeting, “Lab- its effect on patients and their phy-
oratory Data-Its Utilization and Man- sicians,” D. M. Young. Toronto,
agement.” Ont., Canada.