Waste Sheet Template - DOC by qyx19901

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									                                                                                          Revision Date: 8/31/2011
                           Biological Waste Disposal Template
P.I./Lab Supervisor:                                          Lab Location:


Types of Biological Waste Generated:                          Animal Waste:
   Liquid Biological Waste                                       Place animal tissue and carcasses in cooler
   Solid Biological Waste                                        designated by RAR.
   Animal Waste (e.g., tissue, carcasses, bedding, rDNA-         Handle animal cages, bottles, and bedding per
   containing animal waste, transgenic animal, etc)              RAR instructions.
   Toxins
   rDNA (e.g., solid or liquid waste, transgenic plants,      Toxins:
   rDNA-containing plant waste, etc)                             Treat with 2N NaOH for at least 1 hour.
   Sharps                                                        Describe other proven effective inactivating
   Prions                                                       agent.
   Cell Culture / Blood / Body Fluids (e.g., human /
   NHP / animal cells, blood, serum, body fluids, etc)
   Human Organs, Tissue or Body Parts
                                                              Human Organs, Tissue or Body Parts:
Please indicate any other types of biological, or mixed         Call Bequest Program 612-625-1111
biological with other hazardous wastes that will be
generated.                                                    Sharps (contaminated):
                                                                 Place sharps in sharps container, fill to no more
                                                                 than ¾ full, seal and place in the designated
                                                                 waste area in the lab.
NOTE: For each type of waste checked or listed
above, indicate the disposal method below.                    Prions (call waste management at 5-6481 for
                                                              yellow bag and yellow barrel delivery & pick-
Liquid Biological Waste Disposal (including                   up):
infect. or noninfect. agents, rDNA, or cell culture /             Place non-tissue low level solid waste (including
blood / body fluids):                                             animal bedding) in yellow waste bag in yellow
    Autoclave for 30 minutes at 121ºC on liquid cycle.            barrel for incineration.
    Test autoclave monthly with integrator per                    Autoclave liquid waste at 134°C for 1 hour.
    Autoclaving Biological Waste Fact Sheet below.                Wipe instrument for re-use thoroughly clean,
    Disinfect with 10% (1:9 v/v) bleach for at least 30           immerse in 2N NaOH for 1 hour, rinse with
    minutes. OR                                                   water, autoclave at 134-138°C for 18 minutes.
    List other proven effective disinfectant /concentration       Wipe instrument for disposal clean, soak in 2N
    underneath: (minimum 30 minutes contact time).                NaOH for 1 hour at 20°C, then disposal.
    Note: some disinfectants are incompatible with bleach         Place sharps in sharps container, fill to no more
    therefore should not be mixed.                                than ¾ full, seal and place in yellow waste bag
                                                                  incineration.
                                                                  Dispose animal tissue and carcasses in animal
                                                                  digester.
Solid Biological Waste Disposal (including infect.                Describe other proven effective disinfectant.
or noninfect. agents, rDNA, cell culture / blood /
body fluids, or disposable gloves):
    Autoclave for 60 minutes at 121ºC or 125 ºC.
    Test autoclave monthly with integrator per                Other Waste Disposal:
    Autoclaving Biological Waste Fact Sheet below.
    Place biological waste in the red biohazard bag,
    fill to no more than ¾ full, seal and place in the
    designated waste area in the lab.
Waste Disposal Reference:
        Autoclaving Biological Waste Fact Sheet, http://www.dehs.umn.edu/PDFs/autoclaveBioWaste.pdf
        Chemotherapy Drug Disposal Fact Sheet,
         http://www.dehs.umn.edu/PDFs/Chemo%20Waste%20Disposal%20fact%20sheet.pdf
        Environmental Health and Safety’s Waste Flow Chart, http://www.dehs.umn.edu/bio_wastedisptble.htm
        Inactivation of Toxins, http://www.dehs.umn.edu/bio_disposal.htm#inactivation
        Sharps Disposal, http://www.dehs.umn.edu/bio_pracprin_su_ss.htm
        Prion Waste Disposal, http://www.dehs.umn.edu/bio_pracprin_prions_sp.htm
        Hazardous Chemical Waste Management Guidebook,
          http://www.dehs.umn.edu/hazwaste_chemwaste_umn_cwmgbk.htm

								
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