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Fast_ Confident Results for Pesticides - or Forensic Toxicology

VIEWS: 15 PAGES: 35

									Fast, Confident Results for
Pesticides - or Forensic Toxicology Screening
on Day One

                                    p         ,
                                  September 22, 2009
                                        Denise Ibens
                  Solutions Business Development Manager
         What to do if you need:

         • Screen for hundreds of compounds
         • Get results faster
           Run        l
         • R more samples
         • Better sensitivity for your analyses
          Operational i a f
         •O    ti               th
                    l in few months


         And all without additional staff



                                       Analyzers and Application Kits
                                                       Denise Ibens
Page 2                                             September, 2009
         What is at risk?

         Laboratory reputation
         Future funding
         F t    f di
         Regulatory compliance
         Promotion




                          Analyzers and Application Kits
                                          Denise Ibens
Page 3                               September, 2009
         Agilent Analyzers and Application Kits
             - The Fast Track to Greater Productivity

                                           Pesticides
                                  Forensic Toxicology
                                       Semi-Volatiles

                                         Analyzers and Application Kits Denise Ibens

Page 4                                                             September, 2009
What is needed to start or improve an analysis
method?
            Technical
            • New Technologies for better lab
              productivity



            Practical
            P   ti l
            •   Instrument configuration
            •   Application it bilit
                A li ti suitability
            •   Method development
            •   Operator training

                                           Analyzers and Application Kits
                                                           Denise Ibens
Page 5                                                 September, 2009
Agilent Analyzers and Application Kits:
         to simplify new technology start-up
         to     d     th ti   t     li ti      t
         t speed up the time to application mastery.

          Columns                                         Application note
          Consumables                                     Quick Start guide
          Checkout standards                              Video tutorials
          Method of analysis                              Screening Database
          Factory pre-testing                             Application installation
             Note: Content depends on the Application, see ordering details for more information

                                                                                  Analyzers and Application Kits
                                                                                                  Denise Ibens
Page 6                                                                                        September, 2009
Analyzers and Application Kits Reduce the Time
for Method Development.
                                                                                         Sample
                                                                                         ready


                           Standard          Standard
                   Order                                              Develop method
                           checkout        familiarization




         Develop   Order   Application      Application      Refine
         method    Order
                     kit    checkout      familiarization    method




                                         …Faster Application startup


                                                                                       Analyzers and Application Kits
                                                                                                       Denise Ibens
Page 7                                                                                             September, 2009
 Technologies for fast multi-component screening
 with Agilent LC/MS and LC/MS/MS


                         Accurate Mass

          RRLC/UHPLC     Dynamic MRM     MassHunter SW



                                          Jet Stream
                          Databases
          Sample Prep.
                 Prep                     Technology




          T h l i f            t      d ti it i th l b
         …Technologies for greater productivity in the lab
                                                         Analyzers and Application Kits
                                                                         Denise Ibens
Page 8                                                               September, 2009
Improved screening with RRLC/UHPLC                       RRLC/UHPLC
 Technology

• New Agilent 1290 Infinity UHPLC designed to provide highest
  speed, resolution and sensitivity
• ZORBAX Rapid Resolution HT (RRHT) columns for fast and
  ultra-fast HPLC separations with more resolving power.
   p         pp                                    y
• Optimum support of LC/MS on narrow bore columns by low
  delay volume configuration (120 µL)




         …Faster results, better sensitivity and higher data quality
                                                         Analyzers and Application Kits
                                                                         Denise Ibens
Page 9                                                               September, 2009
Improved screening with LCMS Jet Stream                             Jet Stream
                                                                    Technology

Technology

• LCMS Jet Stream technology for ultimate MS sensitivity in
  complex matrices
                        sub femtogram
• Provides femtogram to sub-femtogram sensitivity
• Available on Agilent's new 6460 Triple Quadrupole LC/MS
  and 6530 Accurate-Mass Q-TOF LC/MS,     ,




          …Faster results, better sensitivity and higher data quality
                                                          Analyzers and Application Kits
                                                                          Denise Ibens
Page 10                                                               September, 2009
Improved non-target screening with                              Accurate Mass

                        Q TOF)
Accurate Mass (TOF and Q-TOF)

• ‘Unknown’ screening with sub 2ppm mass accuracy
• Low pico-gram on-column Sensitivity.
            Q            p
• Excellent Quantitative performance.
• Unlimited number of targets > several thousand
• Standards not required for method development
• Retrospective re-analysis of data is possible


                         …all the data available, all the time


                                                     Analyzers and Application Kits
                                                                     Denise Ibens
Page 11                                                          September, 2009
Improved target screening with
                                                                        Dynamic MRM
             Dynamic MRM
MassHunter “Dynamic MRM”

 •Many applications require screening of 100 – 1000
 compounds in one MRM method !
 •WITHOUT Dynamic MRM
 • Need to manually set up multiple time segments to maximize dwell time
 • Tedious and fragile for chromatographic time shifts

 •WITH Dynamic MRM
 • Automatic setup of overlapping time segments without user intervention
 • Less MRMs per time results in longer dwell time / sensitivity
 • Unaffected by chromatographic time shifts


              …Easy method development, better sensitivity
                                                                   Analyzers and Application Kits
                                                                                   Denise Ibens
Page 12                                                                        September, 2009
Improved screening with
                                                      MassHunter SW
MassHunter Software

• intuitive, powerful instrument control, data acquisition,
  qualitative and quantitative data analysis, and reporting.
•      can be used with Agilent time-of-flight (TOF), quadrupole
      time-of-flight (Q-TOF), and triple quadrupole systems.
• software can be complemented by application-specific
  MassHunter software packages that provide stream-lined
  M    H t        ft        k       th t     id t      li d
  operation for specialized analytical tasks such as multi-
  component screening.


             g     p                         g            p
    ... Making MS operation easier—from tuning to final report

                                                          Analyzers and Application Kits
                                                                          Denise Ibens
Page 13                                                               September, 2009
Improved screening with                                                  Databases


Accurate Mass and Dynamic MRM
Databases
• Agilent MassHunter Accurate Mass Personal Compound
  Database utilizes the accurate mass data to provide reliable
   p              qualitative screens.
  ‘positives’ for q
          • Extra Confirmation with Isotope abundance (pattern), spacing and mass
            matching, >7000 Forensic analytes, 1600 Pesticides
• Agilent MassHunter Dynamic MRM Database for Forensics:
          • 200 Forensic compounds, 600 Pesticides
          • Custom databases can be created using the Optimizer SW

     … Better identification and confirmation with less time

                                                                     Analyzers and Application Kits
                                                                                     Denise Ibens
Page 14                                                                          September, 2009
Easy to use LCMS Application Kits
Pesticides or Forensic Toxicology
                                Analysis methods show how to use advanced
                                        g                      p              g
                                technologies for fast multi-component screening
                                                             LCMS Application Kits

     Column & test mix
     Application Note and Quick start guide
     CD with data files and method parameters
     LCMS databases for fast screening:
         Dynamic
        •Dynamic MRM
        •Accurate Mass

   * Contents may vary based upon the application. Ask your Account Manager about the details for your application of interest.

                                                                                                             Analyzers and Application Kits
                                                                                                                             Denise Ibens
Page 15                                                                                                                  September, 2009
Summary: LCMS Target Screening Application Kits


• Easy setup using Agilent Application Kit and Dynamic MRM
  Database
• Highly sensitive QQQ with Agilent Jet stream ESI technology,
  Femtogram range for LODs
• Dynamic MRM functionality, enabling faster chromatography
  with large suite of analytes without the need for segmentation
• Automatic method optimization, for swift method set-up
• Automated Reporting



                                                      Analyzers and Application Kits
                                                                      Denise Ibens
Page 16                                                           September, 2009
Summary: LCMS Non-targeted screening
Application Kits

• Easy setup using Agilent Application Kit and Accurate Mass
  Database
• Full spectral acquisition: all the data-all the time
• Highest TOF Instrument Sensitivity to low pico-gram analyte
  amounts on-column
  Rapid Resolution F t Ch
• R id R                      t     hi Separations f
            l ti Faster Chromatographic S    ti    for
  Quick Sample Turn-around
• Easy data review and reporting using MassHunter Software



                                                         Analyzers and Application Kits
                                                                         Denise Ibens
Page 17                                                              September, 2009
Technologies for fast multi-component screening
with Agilent GC/MS Analyzers
      g                 y


                            Capillary Flow
                           Technology (CFT)
          Retention Time                         Deconvolution
           Locking (RTL)                       Reporting SW (DRS)




                            RTL database         Multimode Inlet



           App. specific
           consumables                        Synchronous SIM/SCAN
                  Prep
           Sample Prep.




           T h l i f            t      d ti it i th l b
          …Technologies for greater productivity in the lab
                                                                    Analyzers and Application Kits
                                                                                    Denise Ibens
Page 18                                                                         September, 2009
   Improved screening                                   Retention Time
                                                         Locking (RTL)

   with Retention Time Locking (RTL)

   • Enables compound confirmation using retention time value
   • Maintain the same retention time after column cutting/change
   • Facilitate inter-laboratory data sharing
   • Precision speed-scalable methods
               speed scalable
   • Makes management of large screening databases possible



          … Get consistent results everywhere, every time

                                                       Analyzers and Application Kits
                                                                       Denise Ibens
Page 19                                                            September, 2009
Improved screening with RTL Database                             RTL database




                               y                  p       p
   • Reduces data review time by > 10 fold for complex samples
   • Databases available for Pesticides, Forensic Toxicology and
      Semi Volatiles
      Semi-Volatiles
   • Broad range screen gives more complete answers
    Reduces need t obtain standards
   •R d        d to bt i t d d
   • Increases the confidence of identification


          … Get consistent results everywhere, every time

                                                       Analyzers and Application Kits
                                                                       Denise Ibens
Page 20                                                            September, 2009
   Improved screening with                                                    Deconvolution
                                                                              Reporting SW
                                                                                 (DRS)

   Deconvolution Reporting SW (DRS)
                                                             National Institute of
      Agilent MSD          Automated Mass Spectral
                                                               Standards and
       Productivity           Deconvolution and
                                                                       (NIST)
                                                           Technology ( S ) Mass
   ChemStation Software     Identification (AMDIS)
                                                           Spectral Search Program
          Quant Results      Compound Identification          Confirmed NIST Hits



          Combined Quantitative and Qualitative HTML Summary Report



                                                       … Better identification
                                                        and confirmation with
                                                        less time


                                                                       Analyzers and Application Kits
                                                                                       Denise Ibens
Page 21                                                                            September, 2009
    Faster Data review with DRS                         Deconvolution
                                                        Reporting SW
                                                           (DRS)




           From Here …
            -- with speed, accuracy and confidence

                     … to Here in 2 – 3 minutes




                                                 Analyzers and Application Kits
                                                                 Denise Ibens
Page 22                                                      September, 2009
                                                                    App. specific
Include sample preparation                                          consumables
                                                                    Sample Prep.
                                                                     QuEChERS




   Improve pesticide screening
        pre-packed
   with pre packed QuEChERS kits

   •International standard compliant kits (AOAC, EN methods)
   • Vacuum-sealed anhydrous foil packs ensures integrity
   • Instructional video and downloadable notes
   • Easy to select ordering guide


                    … Prepare sample with consistency

                                                      Analyzers and Application Kits
                                                                      Denise Ibens
Page 23                                                           September, 2009
   Improved screening                                                                                    Capillary Flow
                                                                                                        Technology (CFT)

   with Capillary Flow Technology (
          p     y                     )
                               gy (CFT)

                                                                              Backflush

      5   10   15   20    25      30      35   40       45   50       55     60    65     70         min



 • Reduce post run bakeout time by backflush heavy matrixes
 • Better analytical result with less chemical background
 • Reduce column and detector maintenance
                         Full scan TIC
                                               SIM
                                               µECD               Optional Detector Splitter
                                               FPD(P)




 • Simultaneously collects MSD & GC selective detectors data
                                         … Getting more data with less time
                                                                                               Analyzers and Application Kits
                                                                                                               Denise Ibens
Page 24                                                                                                    September, 2009
   Improved screening with                                                 Synchronous
                                                                            SIM/SCAN

   Synchronous SIM/SCAN
    y

 • Get SIM and Scan Data in a single run
          - SIM = maximum sensitivity for target compounds
                                           g
          - Scan = best confirmation of targets and identification of
              unknowns
          - For most methods, changing to SIM/Scan results in little, if
              any, degradation in signal-to-noise compared to SIM
              only or Scan only modes


                         … Non-compromising Quantitive
                              and Qualitative result in one run
                                                               Analyzers and Application Kits
                                                                               Denise Ibens
Page 25                                                                    September, 2009
Example Time Savings for Toxicology screening

•Conventional Method:
    – Scan run: 35 min
    – SIM run: 35 min
    – NPD run: 35 min
    – Data Review: ~1 hour
    – Total: 165 min/sample
 F t T M th d
•Fast Tox Method:
    – Scan, SIM, and NPD: 9.75 min
    – Data Review: ~10 min
    – Total: 19.75 min/sample



                                        Analyzers and Application Kits
                                                        Denise Ibens
Page 26                                             September, 2009
Preconfigured and pre-tested GC/MS analyzers:
          ,                  gy
Pesticides, Forensic Toxicology or Semivolatiles


                                                              y
                                                     GCMS Analyzers
                        •Factory pre-configured and pretested using
                        latest GCMS technologies for better lab
                        productivity
                        •Columns, consumables & checkout standard
                        mixture
                        •CD with data files and method parameters
                        •Application Note and Quick start guide
                        •Video tutorials
                        •Application checkout in your laboratory.

   * Contents may vary based upon the application.

                                                                Analyzers and Application Kits
                                                                                Denise Ibens
Page 27                                                                     September, 2009
Summary:

          Agilent GCMS Analyzers for Comprehensive
            Target compound analysis and screening
               g      p         y                g
          • Application ready on day 1
          • Faster analysis and data review
          • Optimum performance
          • Easy to use
            Easy learning of advanced t l
          • E    l    i    f d      d tools


                                              Analyzers and Application Kits
                                                              Denise Ibens
Page 28                                                   September, 2009
          Add Optional Consultancy & Support Services


      Training courses at an Agilent facility


            On site
            On-site applications support
             customized to your needs


            Extended warranty coverage


          Preventive maintenance services



                                                Analyzers and Application Kits
                                                                Denise Ibens
Page 29                                                     September, 2009
Detail information in brochure and web




                                         Analyzers and Application Kits
                                                         Denise Ibens
Page 30                                              September, 2009
Further technical presentations in e-seminars




                 __________
                  October 28,2009




                                          Analyzers and Application Kits
                                                          Denise Ibens
Page 31                                               September, 2009
      Pesticides, Semi-Volatiles and Forensic Toxicology
                Analyzers and Application Kits
                    y            pp


          New technologies b
          N                            i
                 h l i become easier to i l implement
                    and can be setup faster

           Reduce method investigation time and costs

                           lt that
               A l i results th t can b t t d
               Analysis                be trusted
                        from the start


          … Solutions that help you work better and faster,
           ith        li bl      lt
          with more reliable results
                                                      Analyzers and Application Kits
                                                                      Denise Ibens
Page 32                                                           September, 2009
Application Compendium’s




          Food     Environmental   Forensics



             THANK YOU
                                     Analyzers and Application Kits
                                                     Denise Ibens
Page 33                                          September, 2009
          Analyzers and Application Kits
                          Denise Ibens
Page 34               September, 2009
Upcoming e-Seminar


    Forensic and Toxicological Analyses Using the Latest
       LC/MS Technology with Confidence and Speed


                 October 20, 2009– 2:00 p.m. EDT


        Dr. Peter Stone, LC/MS Application Specialist, Agilent
                           Technologies Inc.

								
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