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Fast, Confident Results for
Pesticides - or Forensic Toxicology Screening
on Day One
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September 22, 2009
Denise Ibens
Solutions Business Development Manager
What to do if you need:
• Screen for hundreds of compounds
• Get results faster
Run l
• R more samples
• Better sensitivity for your analyses
Operational i a f
•O ti th
l in few months
And all without additional staff
Analyzers and Application Kits
Denise Ibens
Page 2 September, 2009
What is at risk?
Laboratory reputation
Future funding
F t f di
Regulatory compliance
Promotion
Analyzers and Application Kits
Denise Ibens
Page 3 September, 2009
Agilent Analyzers and Application Kits
- The Fast Track to Greater Productivity
Pesticides
Forensic Toxicology
Semi-Volatiles
Analyzers and Application Kits Denise Ibens
Page 4 September, 2009
What is needed to start or improve an analysis
method?
Technical
• New Technologies for better lab
productivity
Practical
P ti l
• Instrument configuration
• Application it bilit
A li ti suitability
• Method development
• Operator training
Analyzers and Application Kits
Denise Ibens
Page 5 September, 2009
Agilent Analyzers and Application Kits:
to simplify new technology start-up
to d th ti t li ti t
t speed up the time to application mastery.
Columns Application note
Consumables Quick Start guide
Checkout standards Video tutorials
Method of analysis Screening Database
Factory pre-testing Application installation
Note: Content depends on the Application, see ordering details for more information
Analyzers and Application Kits
Denise Ibens
Page 6 September, 2009
Analyzers and Application Kits Reduce the Time
for Method Development.
Sample
ready
Standard Standard
Order Develop method
checkout familiarization
Develop Order Application Application Refine
method Order
kit checkout familiarization method
…Faster Application startup
Analyzers and Application Kits
Denise Ibens
Page 7 September, 2009
Technologies for fast multi-component screening
with Agilent LC/MS and LC/MS/MS
Accurate Mass
RRLC/UHPLC Dynamic MRM MassHunter SW
Jet Stream
Databases
Sample Prep.
Prep Technology
T h l i f t d ti it i th l b
…Technologies for greater productivity in the lab
Analyzers and Application Kits
Denise Ibens
Page 8 September, 2009
Improved screening with RRLC/UHPLC RRLC/UHPLC
Technology
• New Agilent 1290 Infinity UHPLC designed to provide highest
speed, resolution and sensitivity
• ZORBAX Rapid Resolution HT (RRHT) columns for fast and
ultra-fast HPLC separations with more resolving power.
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• Optimum support of LC/MS on narrow bore columns by low
delay volume configuration (120 µL)
…Faster results, better sensitivity and higher data quality
Analyzers and Application Kits
Denise Ibens
Page 9 September, 2009
Improved screening with LCMS Jet Stream Jet Stream
Technology
Technology
• LCMS Jet Stream technology for ultimate MS sensitivity in
complex matrices
sub femtogram
• Provides femtogram to sub-femtogram sensitivity
• Available on Agilent's new 6460 Triple Quadrupole LC/MS
and 6530 Accurate-Mass Q-TOF LC/MS, ,
…Faster results, better sensitivity and higher data quality
Analyzers and Application Kits
Denise Ibens
Page 10 September, 2009
Improved non-target screening with Accurate Mass
Q TOF)
Accurate Mass (TOF and Q-TOF)
• ‘Unknown’ screening with sub 2ppm mass accuracy
• Low pico-gram on-column Sensitivity.
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• Excellent Quantitative performance.
• Unlimited number of targets > several thousand
• Standards not required for method development
• Retrospective re-analysis of data is possible
…all the data available, all the time
Analyzers and Application Kits
Denise Ibens
Page 11 September, 2009
Improved target screening with
Dynamic MRM
Dynamic MRM
MassHunter “Dynamic MRM”
•Many applications require screening of 100 – 1000
compounds in one MRM method !
•WITHOUT Dynamic MRM
• Need to manually set up multiple time segments to maximize dwell time
• Tedious and fragile for chromatographic time shifts
•WITH Dynamic MRM
• Automatic setup of overlapping time segments without user intervention
• Less MRMs per time results in longer dwell time / sensitivity
• Unaffected by chromatographic time shifts
…Easy method development, better sensitivity
Analyzers and Application Kits
Denise Ibens
Page 12 September, 2009
Improved screening with
MassHunter SW
MassHunter Software
• intuitive, powerful instrument control, data acquisition,
qualitative and quantitative data analysis, and reporting.
• can be used with Agilent time-of-flight (TOF), quadrupole
time-of-flight (Q-TOF), and triple quadrupole systems.
• software can be complemented by application-specific
MassHunter software packages that provide stream-lined
M H t ft k th t id t li d
operation for specialized analytical tasks such as multi-
component screening.
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... Making MS operation easier—from tuning to final report
Analyzers and Application Kits
Denise Ibens
Page 13 September, 2009
Improved screening with Databases
Accurate Mass and Dynamic MRM
Databases
• Agilent MassHunter Accurate Mass Personal Compound
Database utilizes the accurate mass data to provide reliable
p qualitative screens.
‘positives’ for q
• Extra Confirmation with Isotope abundance (pattern), spacing and mass
matching, >7000 Forensic analytes, 1600 Pesticides
• Agilent MassHunter Dynamic MRM Database for Forensics:
• 200 Forensic compounds, 600 Pesticides
• Custom databases can be created using the Optimizer SW
… Better identification and confirmation with less time
Analyzers and Application Kits
Denise Ibens
Page 14 September, 2009
Easy to use LCMS Application Kits
Pesticides or Forensic Toxicology
Analysis methods show how to use advanced
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technologies for fast multi-component screening
LCMS Application Kits
Column & test mix
Application Note and Quick start guide
CD with data files and method parameters
LCMS databases for fast screening:
Dynamic
•Dynamic MRM
•Accurate Mass
* Contents may vary based upon the application. Ask your Account Manager about the details for your application of interest.
Analyzers and Application Kits
Denise Ibens
Page 15 September, 2009
Summary: LCMS Target Screening Application Kits
• Easy setup using Agilent Application Kit and Dynamic MRM
Database
• Highly sensitive QQQ with Agilent Jet stream ESI technology,
Femtogram range for LODs
• Dynamic MRM functionality, enabling faster chromatography
with large suite of analytes without the need for segmentation
• Automatic method optimization, for swift method set-up
• Automated Reporting
Analyzers and Application Kits
Denise Ibens
Page 16 September, 2009
Summary: LCMS Non-targeted screening
Application Kits
• Easy setup using Agilent Application Kit and Accurate Mass
Database
• Full spectral acquisition: all the data-all the time
• Highest TOF Instrument Sensitivity to low pico-gram analyte
amounts on-column
Rapid Resolution F t Ch
• R id R t hi Separations f
l ti Faster Chromatographic S ti for
Quick Sample Turn-around
• Easy data review and reporting using MassHunter Software
Analyzers and Application Kits
Denise Ibens
Page 17 September, 2009
Technologies for fast multi-component screening
with Agilent GC/MS Analyzers
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Capillary Flow
Technology (CFT)
Retention Time Deconvolution
Locking (RTL) Reporting SW (DRS)
RTL database Multimode Inlet
App. specific
consumables Synchronous SIM/SCAN
Prep
Sample Prep.
T h l i f t d ti it i th l b
…Technologies for greater productivity in the lab
Analyzers and Application Kits
Denise Ibens
Page 18 September, 2009
Improved screening Retention Time
Locking (RTL)
with Retention Time Locking (RTL)
• Enables compound confirmation using retention time value
• Maintain the same retention time after column cutting/change
• Facilitate inter-laboratory data sharing
• Precision speed-scalable methods
speed scalable
• Makes management of large screening databases possible
… Get consistent results everywhere, every time
Analyzers and Application Kits
Denise Ibens
Page 19 September, 2009
Improved screening with RTL Database RTL database
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• Reduces data review time by > 10 fold for complex samples
• Databases available for Pesticides, Forensic Toxicology and
Semi Volatiles
Semi-Volatiles
• Broad range screen gives more complete answers
Reduces need t obtain standards
•R d d to bt i t d d
• Increases the confidence of identification
… Get consistent results everywhere, every time
Analyzers and Application Kits
Denise Ibens
Page 20 September, 2009
Improved screening with Deconvolution
Reporting SW
(DRS)
Deconvolution Reporting SW (DRS)
National Institute of
Agilent MSD Automated Mass Spectral
Standards and
Productivity Deconvolution and
(NIST)
Technology ( S ) Mass
ChemStation Software Identification (AMDIS)
Spectral Search Program
Quant Results Compound Identification Confirmed NIST Hits
Combined Quantitative and Qualitative HTML Summary Report
… Better identification
and confirmation with
less time
Analyzers and Application Kits
Denise Ibens
Page 21 September, 2009
Faster Data review with DRS Deconvolution
Reporting SW
(DRS)
From Here …
-- with speed, accuracy and confidence
… to Here in 2 – 3 minutes
Analyzers and Application Kits
Denise Ibens
Page 22 September, 2009
App. specific
Include sample preparation consumables
Sample Prep.
QuEChERS
Improve pesticide screening
pre-packed
with pre packed QuEChERS kits
•International standard compliant kits (AOAC, EN methods)
• Vacuum-sealed anhydrous foil packs ensures integrity
• Instructional video and downloadable notes
• Easy to select ordering guide
… Prepare sample with consistency
Analyzers and Application Kits
Denise Ibens
Page 23 September, 2009
Improved screening Capillary Flow
Technology (CFT)
with Capillary Flow Technology (
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gy (CFT)
Backflush
5 10 15 20 25 30 35 40 45 50 55 60 65 70 min
• Reduce post run bakeout time by backflush heavy matrixes
• Better analytical result with less chemical background
• Reduce column and detector maintenance
Full scan TIC
SIM
µECD Optional Detector Splitter
FPD(P)
• Simultaneously collects MSD & GC selective detectors data
… Getting more data with less time
Analyzers and Application Kits
Denise Ibens
Page 24 September, 2009
Improved screening with Synchronous
SIM/SCAN
Synchronous SIM/SCAN
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• Get SIM and Scan Data in a single run
- SIM = maximum sensitivity for target compounds
g
- Scan = best confirmation of targets and identification of
unknowns
- For most methods, changing to SIM/Scan results in little, if
any, degradation in signal-to-noise compared to SIM
only or Scan only modes
… Non-compromising Quantitive
and Qualitative result in one run
Analyzers and Application Kits
Denise Ibens
Page 25 September, 2009
Example Time Savings for Toxicology screening
•Conventional Method:
– Scan run: 35 min
– SIM run: 35 min
– NPD run: 35 min
– Data Review: ~1 hour
– Total: 165 min/sample
F t T M th d
•Fast Tox Method:
– Scan, SIM, and NPD: 9.75 min
– Data Review: ~10 min
– Total: 19.75 min/sample
Analyzers and Application Kits
Denise Ibens
Page 26 September, 2009
Preconfigured and pre-tested GC/MS analyzers:
, gy
Pesticides, Forensic Toxicology or Semivolatiles
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GCMS Analyzers
•Factory pre-configured and pretested using
latest GCMS technologies for better lab
productivity
•Columns, consumables & checkout standard
mixture
•CD with data files and method parameters
•Application Note and Quick start guide
•Video tutorials
•Application checkout in your laboratory.
* Contents may vary based upon the application.
Analyzers and Application Kits
Denise Ibens
Page 27 September, 2009
Summary:
Agilent GCMS Analyzers for Comprehensive
Target compound analysis and screening
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• Application ready on day 1
• Faster analysis and data review
• Optimum performance
• Easy to use
Easy learning of advanced t l
• E l i f d d tools
Analyzers and Application Kits
Denise Ibens
Page 28 September, 2009
Add Optional Consultancy & Support Services
Training courses at an Agilent facility
On site
On-site applications support
customized to your needs
Extended warranty coverage
Preventive maintenance services
Analyzers and Application Kits
Denise Ibens
Page 29 September, 2009
Detail information in brochure and web
Analyzers and Application Kits
Denise Ibens
Page 30 September, 2009
Further technical presentations in e-seminars
__________
October 28,2009
Analyzers and Application Kits
Denise Ibens
Page 31 September, 2009
Pesticides, Semi-Volatiles and Forensic Toxicology
Analyzers and Application Kits
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New technologies b
N i
h l i become easier to i l implement
and can be setup faster
Reduce method investigation time and costs
lt that
A l i results th t can b t t d
Analysis be trusted
from the start
… Solutions that help you work better and faster,
ith li bl lt
with more reliable results
Analyzers and Application Kits
Denise Ibens
Page 32 September, 2009
Application Compendium’s
Food Environmental Forensics
THANK YOU
Analyzers and Application Kits
Denise Ibens
Page 33 September, 2009
Analyzers and Application Kits
Denise Ibens
Page 34 September, 2009
Upcoming e-Seminar
Forensic and Toxicological Analyses Using the Latest
LC/MS Technology with Confidence and Speed
October 20, 2009– 2:00 p.m. EDT
Dr. Peter Stone, LC/MS Application Specialist, Agilent
Technologies Inc.
