13-3 Cell Transformation

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13-3 Cell Transformation Powered By Docstoc
					   Chapter 13
Genetic Engineering
        Section 13-3
    Cell Transformation

  FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
     Cell Transformation
Key Concept:
       During Transformation
1. A Cell Takes In DNA From Outside
   The Cell.

2. This External DNA Becomes Part Of
   The Cell’s DNA.

       FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
   Transforming Bacteria
• Plasmids
 –Found In Bacteria
   • Small Circular Pieces Of DNA
   • Has DNA Sequence That Functions
     As Origin For Replication
     –This ensure that once in the bacteria,
      it will be replicated


     FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
    Transforming Bacteria
• Plasmids (cont.)
  – Contains A Genetic Marker
    • A Gene That Labels The Bacteria As A
      Plasmid Carrier
       –For Example: Resistance To
         Antibiotics
          »Allows Transformed Cells To Be
           Identified



       FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
Transforming Bacteria




  FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
  Transforming Plant Cells
• Transform Bacteria With Plant Tumor
  Plasmid (Agrobacterium tumefaciens)
  – Scientists Inactivate Plant Tumor Gene
  – Insert Foreign DNA
  – Recombinant Plasmid Then Transforms
    Plant Cells




        FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
  Transforming Plant Cells
• Cultured Plant Cells With Cell Walls
  Removed Will Sometimes Absorb DNA
• DNA May Be Injected Directly Into Plant
  Cells (Gene Gun)
• Cells Then Grown Into Adult Plants




       FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
  Transforming Plant Cells
Key Concept:
   If Transformation Is Successful, The
   Recombinant DNA Is Integrated Into
   One Of The Chromosomes Of The
   Cell




       FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
Transforming Plant Cells




   FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
  Transforming Animal Cells

Egg Cells Large Enough:
 – DNA Injected Into Nucleus
   • Enzymes Normally Responsible For
     DNA Repair & Recombination May Insert
     DNA Into Chromosomes




      FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
  Transforming Animal Cells
• Recombinant DNA Contains Markers
  Allowing Transformed Cells To Be
  Identified




       FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
  Transforming Animal Cells
Scientists Can Knock Out Single Genes

 – Flanking Sequences In New DNA Can
   Remove A Host Cell Gene or Replace
   It With A New Gene




      FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT
Knocking Out Genes




 FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT

				
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posted:9/8/2011
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