Are We Feeding Our Cows Too Much Protein?
Degradation
Feed Proteins
(Esophagus)
Peptides & Free AA
World Dairy Expo October 2005
URINE
Feed NPN
Microbial Synthesis
Escape
(Omasal orifice)
MICROBE PROTEIN Ammonia overflow
NH 3
N-Recycling
LIVER
UREA
Glen Broderick U.S. Dairy Forage Research Center Madison, Wisconsin
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Dairy Cows Uses Crude Protein 2-3 X More Efficiently than Beef Animals
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Feed N utilization Manure N utilization
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How can Nitrogen Excretion be Reduced Without Losing Production?
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Strategies to Reduce N Excretion
1. 2. 3. Feed the Minimum Crude Protein (CP). Accurately Track Dietary CP. Use Model (e.g., NRC) to Meet Needs for RumenDegraded Protein (RDP) & Rumen-Undegraded Protein (RUP). Reduce Rumen Degradability of Forage CP. Feed “Complementary” RUP & Protected AA. Use New Approaches to “Fine-Tune” Feeding.
4. 5. 6.
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Effect of CP (Solvent SBM) or Energy on Protein Yield
2.50
-----32% NDF----b b -----28% NDF----a a a
Milk protein, lbs/day
2.25
-----36% NDF----cd cd c
2.00
d
1.75
1.50
15.1 16.7 18.4 15.1 16.7 18.4 15.1 16.7 18.4
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Dietary CP, %
Effect of CP (Solvent SBM) on Milk & Protein Yield (Olmos & Broderick, 2003)
85 Milk yield max = 16.7% CP
Milk yield, lbs/day
80
2.75
Milk protein, lbs/day
75 Milk protein max = 17.1% CP
2.50
70 13 14 15 16 17 18 19
2.25 20
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Dietary CP, % of DM
Effect of Forage Source & CP on Production
(Wattiaux & Karg, 2004) Forage/CP (%) Alfalfa silage Corn silage 16.5 18.0 16.2 17.1 53.8 101.6 101.6 3.77 2.80 11.7 55.8 103.0 102.3 3.51 2.80 12.2 53.4 108.7 101.4 3.35 2.91 11.5 54.0 107.4 101.2 3.26 2.89 12.8 Prob.1 For. Prot. 0.41 0.03 0.84 0.08 0.20 0.35 0.30 0.97 0.95 0.35 0.88 < 0.01
Item DMI, lb/d Milk, lb/d 3.5% FCM, lb/d Fat, lb/d True protein, lb/d MUN, mg/dl
1No
significant Forage*Protein interactions were observed (P > 0.60).
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Effect of Dietary CP on the Lactation Curve
(Wu & Satter, 2000)
Symbol wk 1-16 15.4% 17.4% 17.4% 19.3%
wk 17-44 16.0% 16.0% 17.9% 17.9%
FCM 23,570b 25,640a 26,020a 25,480a
Manure N 279c 309b 358a 355a
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Strategies to Reduce N Excretion
1. Feed the Minimum Crude Protein (CP). 2. Accurately Track Dietary CP. 3. Use Model (e.g., NRC) to Meet Needs for RumenDegraded Protein (RDP) & Rumen-Undegraded Protein (RUP). 4. Reduce Rumen Degradability of Forage CP. 5. Feed “Complementary” RUP & Protected AA. 6. Use New Approaches to “Fine-Tune” Feeding.
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Sampling Forage is Most Important
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Changes in CP Over a 17-Week Trial
Alfalfa silage Corn silage High moisture corn 48% Soybean meal
55
Crude protein, % of DM
50
25
10
5 1 3 5 7 9 11 13 15 17
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Week of Experiment
Relationship of Dietary CP to MUN
(Nousiainen et al., 2004)
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Strategies to Reduce N Excretion
1. Feed the Minimum Crude Protein (CP). 2. Accurately Track Dietary CP. 3. Use a Ration Formulation Model (e.g., NRC) to Meet Needs for Rumen-Degraded Protein (RDP) & RumenUndegraded Protein (RUP). 4. Reduce Rumen Degradability of Forage CP. 5. Feed “Complementary” RUP & Protected AA. 6. Use New Approaches to “Fine-Tune” Feeding.
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NRC (2001) Protein Model
1. Meet Microbial Needs for RDP. 2. Supply Metabolizable Protein to Meet Cow Needs with RUP. 3. Match Protein Quality to Cow’s Requirements (Not All RUP sources are Equal).
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Matching Rumen Available Energy with RDP
Get The Most Bang Out of Your Dietary Carbohydrate (But Not Too Much)
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Effect of Processing on Digestion of Corn Starch (Owens et al., 1986)
Processing Method Proportion of Starch Digestion, % Rumen Small Large Total Intestine Intestine tract 13 14 6 8 4 1 89 94 95
Cracked Corn 69 78 Ground Corn High Moisture Corn 86
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Rumen Ammonia & Production of Cows fed Alfalfa Silage & Ground HMEC (Ekinci & Broderick, 1997)
25 HMEC (4.3 mm)
Milk Yield (lbs/day) 70 + 5.3 + 0.26 2.2 Protein Yield (lbs/day)
Rumen ammonia, m M
20
65
2.0
15
60
1.8 Milk Protein HMEC Ground HMEC
10
5 Ground HMEC (1.7 mm) 0 0 6 12 18 24
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Time after feeding, h
Effect of Replacing Alfalfa Silage with Concentrate (Valadares et al., 2000) 100
Milk & FCM yield & DM intake, lbs/day
90 3.5% FCM
80
Milk
Fat & Protein yield, lbs/day
70
Fat Dry Matter Intake
3.5
60
3.0
50
Protein
2.5
40 20 35 50 65
2.0
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Dietary concentrate, % of DM
Over-Feeding Grain Depresses Rumen pH & Milk Fat (Valadares et al., 2000)
6.75 3.75
Milk Fat Content, %
Rumen pH
6.50
3.50
6.25 Rumen pH Milk Fat, % 20 35 50 65
3.25
6.00
3.00
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Dietary Concentrate, % of DM
Lameness from Over-Feeding Grain
Too Little Fiber in the Diet Results in Release of Histamine & Metalloproteinases, leading to Foot & Leg Damage.
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Replacing Alfalfa Silage with Corn Silage
(Brito & Broderick, 2003)
Alfalfa Silage:Corn Silage 37:13 24:27 10:40
Item
51:0
Composition (% of DM) Alfalfa Silage Corn Silage Crude Protein Production DM Intake (lbs/d) Milk Yield (lbs/d) Rumen NH3 (mg N/dl)
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50.5 0 17.2 58.4a 91.5a 10.5a
. .
37.1 13.3 17.0 57.1a 92.6a 10.0ab
. .
23.6 26.7 16.8 55.1b 91.5a 8.7b
.
10.2 40.0 16.6 51.1c 87.1b 6.2c
. .
a,b,c(P
< 0.05)
Feeding Sugar with Alfalfa Silage
1. Replacing Dietary Starch with ~2.5% Sugar Increased Intake, OM Digestibility & Fat Yield. 2. Reduced Rumen Ammonia. 3. Small Effects on Milk & Protein Yield.
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Strategies to Reduce N Excretion
1. Feed the Minimum Crude Protein (CP). 2. Accurately Track Dietary CP. 3. Use Model (e.g., NRC) to Meet Needs for RumenDegraded Protein (RDP) & Rumen-Undegraded Protein (RUP). 4. Reduce Rumen Degradability of Forage CP. 5. Feed “Complementary” RUP & Protected AA. 6. Use New Approaches to “Fine-Tune” Feeding.
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Harvest Alfalfa as Silage or Hay?
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Mean Composition of Alfalfa Silage & Hay
Item DM, % CP, % DM NPN, % CP Est. RDP, % CP NDF, % DM NEL, Mcal/kg DM Ash, % DM
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Silage 41 20.6 51.9 71 38 1.51 10.4
Hay 86 18.1 8.0 73 38 1.50 9.8
Change, % ---12 -85 NS NS NS -6
Photo are nee
Production on 50% Alfalfa Silage or Hay +/- Fish Meal (FM) Supplementation (Vagnoni & Broderick, 1997)
+4.0 90 89.7
Alfalfa Silage
+0.5
a
Alfalfa Hay Alfalfa Silage + FM Alfalfa Hay + FM
a
86.6
a
3.0
+0.22 2.80
b
85
a
+0.04
a
a
2.65
2.8
80
b
2.6
75
2.4
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Milk yield, lbs/day
Protein, lbs/day
Strategies to Reduce N Excretion
1. Feed the Minimum Crude Protein (CP). 2. Accurately Track Dietary CP. 3. Use Model (e.g., NRC) to Meet Needs for RumenDegraded Protein (RDP) & Rumen-Undegraded Protein (RUP). 4. Reduce Rumen Degradability of Forage CP. 5. Feed “Complementary” RUP & Protected AA. 6. Use New Approaches to “Fine-Tune” Feeding.
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CP Supplement & Production
(AS, CS, HMSC, 16.5% CP; Brito, 2004) Diets1 SSBM CSM Item Urea CM
P>F
---------------(lbs/day)--------------DM intake Milk yield Milk protein Milk fat
1SSBM
48.7c 72.5b 2.03c 2.23c
53.4b 88.2a 2.71ab 2.69ab
54.5ab 89.3a 2.60b 2.60b
54.9a 90.6a 2.80a 2.84a
< 0.01 < 0.01 < 0.01 < 0.01
= Solvent Soybean Meal; CSM = Cottonseed Meal; CM = Canola Meal a,b,c(P < 0.05)
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CP Supplement & Omasal Protein Flows
(Brito, 2004) Diets1 SSBM CSM Item RUP (“Bypass”) Microbial protein Total protein
1SSBM
Urea
CM
P>F
-----------------g/d-------------------538c 987b 1348a 1150ab <0.01 2706a 2706a 2775a 0.04 2344b 2882c 3693b 4054a 3925ab <0.01
= solvent soybean meal; CSM = cottonseed meal; CM = canola meal a,b,c(P < 0.05)
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Strategies to Reduce N Excretion
1. Feed the Minimum Crude Protein (CP). 2. Accurately Track Dietary CP. 3. Use Model (e.g., NRC) to Meet Needs for RumenDegraded Protein (RDP) & Rumen-Undegraded Protein (RUP). 4. Reduce Rumen Degradability of Forage CP. 5. Feed “Complementary” RUP & Protected AA. 6. Use New Approaches to “Fine-Tune” Feeding.
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Supplementing Rumen Protected-Met While Decreasing CP
Item CP, % 18.6 RP-Met, g/d 0 Milk, lbs/d 87.7ab Milk/DMI 1.72ab Protein, lbs/d 2.54 MUN, mg/dl Milk N/NI, % 14.5a 26c 17.3 8 91.7a 1.80a 2.71 11.8b 30b 16.1 17 91.9a 1.77ab 2.71 9.4c 32b 14.8 25 87.5b 1.69b 2.65 7.9d 34a P>F 0.06 0.06 0.19 < 0.01 < 0.01
< 0.05) RP-Met = Mepron
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a,b,c,d(P
New Approaches to Consider
1. Protected Amino Acids
• • •
Rumen-Protected Met Sometimes Effective. Rumen-Protected Lys (Feeding with Corn Distillers). Other Possible Zero-N “Amino Acids” (RP-MHA).
2. Slow-Release RDP (Bugs Fed N at “Right Rate”)
• •
Slow-Release RDP from True Protein. Alfalfa Hay has Slow Release RDP vs. Alfalfa Silage.
3. Suppressing Rumen Protozoa
• • USDFRC
Protozoa Waste Protein but Improve Rumen Stability. Suppress Protozoa & Use Rumen Supplements.
Same Milk for Less Crude Protein
1. More Profits, Less Pollution, Better Manure 2. How Much Over-Feeding of Protein is Risk Management (“Safety Margin”)? 3. How Low Can We go & Maintain Production?
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