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Improvement of Rainfed Lowland Rice Variety for Cooking Quality

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					Improvement of Rainfed Lowland Rice Variety
for Cooking Quality Traits using MAS



            Sureeporn Katengam
             Uraiwan Kotchasatit

         UBONRATCHATHANI, THAILAND
Background
   Rice production in NE of Thailand
   Area of production ranging from
    drought-prone lands to flooding area
   Abiotic stresses related with
    unpredictable rainfall pattern are main
    problems
State of problem
   Abiotic stresses associated with
    unpredictable rainfall pattern are main
    problems for rice production in NE of
    Thailand.
   Elite cultivars resistance to drought are
    required.
State of problem (cont.)
   KDML105, an aromatic rice with good cooking
    quality, is widely grown in NE, however, its
    production is dominating constraint with the
    drought problem.
   Drought tolerance is a complex traits since
    this trait is governed by many QTLs locating
    in many chromosome (ch1, ch2, ch3, ch4,
    ch7, ch8,and ch9).
   Therefore, improvement KDML105 for
    drought resistance is not an easy tasks.
State of problem (cont.)
   In the opposite way, improving of well adapted
    variety to drought prone areas for other quality
    traits such as cooking quality is worth to
    reconsider.
   Cooking quality trait is determined by three
    characters specifying the physical properties of
    starch in the endosperm:
          Amylose content (AC)
          Gel consistency (GC)
          Gelatinization temperature (GT)
Objectives
   Marker-assisted backcross breeding is aimed
    at improving well drought adapted variety to
    rainfed lowland area of NE of Thailand for
    cooking quality traits utilizing tightly linked
    DNA markers.
   The major QTL controlling AC, GC, and GT
    are located on Ch.6. DNA marker of flanking
    markers closely linked to this major QTL
    controlling quality traits will be carried out for
    MAS.
Plant materials
   Donor parent:
       KDML105
           low AC (~16-17%): major QTL on ch6 (at Wx gene )
           low GT: major QTL on ch6
           medium GC: major QTL on ch6 (at Wx gene )
   Recipient parent:
       IR57514-drought
Breeding scheme:
             Genetic map (Azu / IR64 )

        Chr.6                        Chr.11




Map location of QTLs controlling cooking quality traits
     Survey of SSR markers for parent screening
                                                         Expected size (bp)
#     Markers Traits                    Chrom KDML 105 IR57514 KD571-77Size expected Remark
1      BADH     Aroma                      8     ~ 400         ~410           ~400             Polymorphic
2     RM190     Amylose content            6      ~120         ~116           ~120      124    Polymorphic
3      Waxy     & Gel consistency          6      ~311         ~315           ~311             Polymorphic
4     RM204                                6      ~155         ~119           ~155      169    Polymorphic
5     RM205                                6       121          121            121            Monomorphic
6     RM225                                6      ~145         ~120           ~145      140   Polymorphic
7     RM111     Gelatinization             6       120          120            120      124   Monomorphic
8      RM50     temperature                6       200          200            200      201   Monomorphic
9     RM121                                6      ~275         ~272           ~275             Polymorphic
10    PSS-1-1                              6       149          149            149            Monomorphic
11    PSS2-2                               6       200          no             200
12    RM136                                6       290          290            290      101   Monomorphic
13     GT11                                6      ~200         ~195           ~200             Polymorphic
14    RM229                                11     ~119         ~119            200      116         ?
15    RM457                                11     ~230         ~232           ~230      230    Polymorphic
16     RM21                                11      no           no              no      157         ?
17    RM473E                               11    141, 139   122,119,118        118            Multiple bands
                                                                117           117,116            (repeat)
18    RM206                                11     ~160         ~148           ~120      147     ? (repeat)
19    RM254                                11     ~156         ~156           ~153      165         ?
20     PB7-8    Bacterial leaf blight(Xa21) 11     no           no             no              ? (repeat)
21    RBO 783   Submergence tolerance      9       375         ~350           ~350            Monomorphic
    Screening parents with SSR markers




                                         PSS II-1
                                         PSS II-2
                                RM473E




                                                           WAXY
                                                    BADH
        RM205
        RM204




                        RM229
                        RM457


                                RM206
                                RM254




                                                                         RM121
        RM597
        RM190



        RM225
        RM111

                RM121
                RM136




                                RM21




                                                                  RM21
                RM50
                                                                                 PCR condition




                GT11
726bp

553bp

        1
        2
        3
500bp

427bp


311bp


249bp


200bp
                                                                                 Thermal cycling

151bp
140bp


118bp

                                                                                      1 KD
100bp                                                                                 2 IR57514
                                                                                      3 KD571-77
SSR markers chosen for parents screening

    # Markers          Traits                              E
                                      Chrom IR57177 KD571-77 xpected size (bp) Remark
    1   BADH            Aroma            8     ~410     ~400

    2   RM190      Amylose content       6     ~116     ~120        124

    3   Waxy      & Gel consistency      6     ~312     ~311

    4   RM204                            6     ~119     ~155        169

    5   GT11        Gelatinization       6     ~195     ~200

    6   PSS2-2       temperature         11                                    Agarose

    7   RM457                            11    ~232     ~230        230

    8   RM21                             11                         157        repeat

    9   RM206                            11                         147        repeat

   10   PB7-8 Bacterial leaf blight(Xa21) 11                                   Agarose
2nd workshop on December
     MAS for BC1F1
                                   Planting parents                          early-April

                            donor               recipient
                            KD571-77   X        IR57514                  late-June


                                           F1                            mid-July

                           Planting F1 and recipient parents                 early-August


                                           F1 X IR57514                  mid-October


                                           BC1F1                         mid-November

    Line selection with                     MAS
    selected SSR markers

                           1 2 3 4……………………………….             .200 lines       late -November

				
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posted:9/5/2011
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