Gene FAM134A FAM134A FAM134A FAM134A CYP27A1 CYP27A1 CYP27A1 CYP27A1 CYP27A1 CYP27A1 CYP27A1 CYP27A1 SLC23A3 SLC23A3 SLC23A3 SLC23A3 SLC23A3 SLC23A3 SLC23A3 SLC23A3 SLC23A3 SLC23A3 SLC23A3 WNT10a C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24 C2orf24
Primer Pair F4/R4 F5/R5 F9/R9 F10/R10 F1/R1 F2/R2 F3/R3 F4/R4 F5/R5 F6/R6 F7/R7 F8/R8 F3/R3 F4/R4 F6/R6 F7/R7 F8/R8 F9/R9 F10/R10 F11/R11 F15/R15 F16/R16 F17/R17 F10/R10 F1/R1 F2/R2 F3/R3 F4/R4 F5/R5 F6/R6 F7/R7 F8/R8 F9/R9 F9/R9a F11/R11 F12/R12 F14/R14 F16/R16 F17/R17 F18/R18
Forward GAGTGCCTAGGAAGCTGGTG CTGCGTTTGGCATCTGAATA GCCAGAGACATCCTCAGAGC CACTGCTCTGTTCCCTCCTC CTGACAGCAAGCTGCCTTAG CCTAGGCTCTCTTGGGGTCT GGCTACCTGCACTTCTTGCT TTTTCCTTTTGTCGCGTGTG GTTTTCCTTTTGTCGCGTGT AGGGAGGGCAGTGGGTAG CCTCTCCCCTCTTAGGGATCT ACTCACAAGAGCGTGGTTCC CAGGACAGCCAAGACAAGAA CTGCTCCTGCTTCAAGGACT TGGTCTGTTCTCAGCACCTG CCCAACACAGCTCTTTCTCC CACTAAGCCACCGTTCCATT GACTCTTGGGTCCCATTTGA ATGGGGACTGAGATGGTCTG GGATTCCTGTGGCACAAAAC TCCGAAAAGGCTGAAAACTG GACTCCGAAAAGGCTGAAAA GTGGTGAGGAGGGAACACTG TCCTGTGCTATCCTGTGCTG CCCTGAGCTCTTGAGACACC GGAGTTGCTTGCCTATCTGC CTGTCCTGCCTGTTGGCTAT GGCATATGTGAGCAGTGTGG GCCTTTCCCTTTTGTCTTCC CCCTCTTCCCCTGGTTCTTC CTCACCGGCTTCCAGGACT AGTCTAACATCGGGCTCCTG CAGCTCACACAGCCTGACAT CAGCTCACACAGCCTGACAT AGGGCACCTTCTGCCTCAC TTGGCAAATAATCCCGAGAA TGGAATGGGCTTTTTGTCTC GTTAGCTGAGGGGCGTACTG AGCCCGTCTCAAGTCTTTCA TGCAGACTCCCCTTGTCTTT
Reverse CCAGAACAAACTCGAGCACA ACCAGCCCTCTTCCTTCAGT ACCACACTGGGAACATCCAT TAATGGACAGGGGCTTTCTG CAGCTCACGTGGTGAAGG CACTTCCTTCCCTGGCTTC TGGGATCCCTCTCACAAAAG AGCTCTTCAGCCCCACTCAC GCAGGGAAAAGAAAGTGCAG TGGGTGTCCCTCTAGGTGTC AGCCTGCTTTCCTTCTGTTG GAGGGTAATGGGGAATTGGT AGAGGCAGCCTGTAGGAACA ACCATCTCAGTCCCCATCAG CCCTCAGACTGGCAATTTCT CCTGAAGGGTCCTCCTTTTC TTTCCAGTTACGCCAAAAGC ATCCTTCCTCCTCCCACCT GAAATGAGGATGGAGGACGA GCTCACTGCTATCCGTGTCA AGAGGGCTTCTCAGGTCACA AGGTCCATGGATCGGAGTT AAATTTGCAGGATGGTGGAC GCCACAAGAGAGGGACTCTG AGGACCATGGCACCAACTAC GCTACTCACCATGGCACTCA CCTCCAGGTTCTCAGGGACT CCTAGGAGGACAGGGACCAG AGAGGACCCAAGTCAAGCAA ATGAGGGGACGTTGGAAATG TCGGGATTATTTGCCAAAGG CAGCTCAAAATCAGTGTTACCG TCACCGTGAAGTCCTGGAA GAGGCTACGGTGGAGATCCT GGTGTCCTGGGAGGAACTGA GCAGATAGGCAAGCAACTCC AGAAGCGGTGTCTCAAGAGC AGCCCCAAGGACTGATGAAT GACCCGAAACCAAAAGTTGA CAATGGTGGAGGTGTCAGTG
Annealing Temp 64C 60C or 62C 64C 64C 60C 60C 60C 60C 60C 60C 64C 64C 64C 62C 62C 62C 62C 62C 62C 62C TD 66-60 TD 66-60 TD 66-60 62C 62C 62C 62C 62C 62C 62C 62C 62C 62C 62C 64C TD 66-60 62C 62C 64C 62C
Extension Time 45sec 45sec 45sec 45sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 45sec 1'30sec 30sec 30sec 45sec 45sec
IHH IHH IHH IHH IHH IHH IHH between CYP27A1 and PRKAG3 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 NHEJ1 PRKAG3 PRKAG3 PRKAG3 PRKAG3 PRKAG3 PRKAG3 PRKAG3 FEV FEV FEV FEV FEV FEV CRYBA2 CRYBA2 CRYBA2 CRYBA2 CRYBA2
F1/R1 F2/R2 F3/R3 F4/R4 F5/R5 F6/R6 F7/R7
GCTAGGCGCTAACCTTACCA TTGTCAGCGTCTCTGTCACC ATGCCCTGATCTGACCATTC TGTCCCTTCTTCCCACAGAG ATGCGAGGAAGCAAGTTAGG CACACCATTGCAACTTGACC ACTAACAAGGCACGGGACAC
TTGCTTCCTCGCATTCTAGC GGCACCAAGACCTAGGAGTG CTGTGTGGTTGTTGGAGGTG CCAACTCAGATCCCTTCCAG GCAGGAACCATTTGAAGCAT TGAGGTCAGCATGTGAAAGC GCAGCATCGACTGAGGTGTA
62C 62C 62C 62C 62C 62C 62C
45sec 45sec 45sec 45sec 45sec 45sec 45sec
F1/R1 F4/R4 F5/R5 F6/R6 F7/R7 F9/R9 F10/R10 F11/R11 F12/R12 F13/R12 F14/R14 F15/R15 F16/R16 F17/R17 F1/R1 F2/R2 F3/R3 F4/R4 F5/R5 F6/R6 F7/R7 F2/R2 F4/R4 F5/R5 F6/R6 F7/R7 F8/R8 F1/R1 F3/R3 F5/R5 F7/R7 F9/R9
TGAGATGGGAGCTGTGTCTG CGTAGGGTGGACAGGACACT CTCCTGAACAGTGCTTGTGG CCATCCTCCAAACACAAGGT GGACAGGCAACCTCACTTGT GAAAGGCTAGCGTTGGCATA GTGGGGCCACTTTCCTAAAT AGCGAGCCAAGGTAAGTTGA AAGGCAGATGCTCAACCACT GGGAGGTCAGATCAGGTTGA AGCCCTGAAATGGCTTGTAA TGGCTGATTGATATCTCTTCCA ATCCCTTCTCCCACTGGACT TCTCACAGGCAGAAAGCTCA CAAGCTGGTCATCTTCGACA GAAAGAAGCCATGGGAAACA TTCCTAGCACCATGGAGACC CCCAGAGCTTAAGGTGCGTA TGTAGGAACAGCACGCAGAG GATCTGTGGGTTCAGGTGGT GGAAGCCTAAAAGGGGTGAC CTGGGAACCACATCAGCTTT CTGGAAAGGCAAGAAACAGG TGCGCGTTGGAGACTTTATT CGGGAGGACTGAGCAATCTA GTTTCCTGACTTGGGCGTTA AGGGAAGGAGGCATTCACTT CCTCGTGTTTTAGGGCACTT GCTGGTGGGAACAACAAAAT TGGGCCTCAGCAGTTTCTAT CAGAGAATTCCTTCCAACCTG GCCCTGACCTAGAAGGGACT
TGACTGGCATTTCTTTCGTG CCTAAGCTGTGACCCTTTGC GCCTTAGGGTGTCCTCCAAT TTCCGGAAAAGAGGGATTCT TGGGACGAAGAGGTCCATAG CACCCCAACCCTCACTAAGA CATTCGCTGGAATTGTCTCA GAGTCGGGGTGTAACAAGGA TCCCCACTCCACACTTGAAT TCCCCACTCCACACTTGAAT AGCCCCACTCTCCTGGTAGT CTTCAAATGGCTCCGTCTTC AATTTGATCGACTGGGCAAC CCATTCATTCCTTTGCCAGT CCCTGCTCTGTGTGATGCTA AGGGTAGGCCTCTCTTCCAC GCAGCGTTTCCACTAGGAAG CTTCCTCTGCTGGAAGTTGG CGGATCGGTTTCTCTCTGAT TCTGGGTGATCAGCTGTCTG AGGGCGTAGACAGCTTCAAA GCAGCAGTTGCTCCCTTTAT CCTGGCAGGTACATGTTGAT CCCTACCAGGCTCTCCAAG TGCAGCAGGAGAAAACACTC GGTAGGTGCTCACTCGGTTC AGAGGCTTGGAAACCCATTT GCTTGTAGAGGCCAGATGCT GCCTACCCTCCACTTCTTCC CCTCTCGCCTTTATCCCTTT CCTGATATAGGCCCCAGTCC TTAGCACCCCATCACCTTTC
64C 64C 64C TD 66-60 TD 66-60 TD 66-60 TD 66-60 TD 66-60 62C TD 66-60 TD 66-60 62C 62C 62C 62C 60C 62C 62C 60C 60C 60C 60C 60C 60C 60C 60C 60C 62C 62C 62C 62C 62C
30sec 30sec 30sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 45sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec 30sec
CRYBA2 ABCB6 ABCB6 ABCB6 ABCB6 STK16 STK16 CDK5R2 CDK5R2 CDK5R2
F11/R11 F1/R1 F2/R2 F3/R3 F4/R4 F1/R1 F2/R2 F2/R2 F4/R4 F5/R5
TGTGGGGAGGCAGGTAATAG GGTATTGTTCCAGAAGTGCCTTA AGAGGGGCCAGATTGAGTTT TCCTCCTCCAGCTTGTCCTA CACCCTGCTTCAGCTACCTC CAAGCGCTACCTCTTCATCC AGGTTATGCCCACAGGTCAG GCACGGTGCTGTCTCTTTC ATCTCCGTCTTCCTCCCTGT AGACCCCGACACGCACTC
AGGCAGTTTATGCTCCAGTCA AGGGGGAGGTCTGCTACCTA AGGGGTGACATCTCCTCTCC GGAAAGCCCTTTCAGAAACC CATACCCAAGGCAGTCCCTA GGCCCTGGGTGAGATTATTA GGTCCAGAGAAAGCATCAGC GGAGAAGGTTCTCGCGATT CTCCCTCTGGAAACAACCAA GGCCTCAAATAGCACCTCAGA
62C TD 66-60 64C 64C 64C TD 66-60 TD 66-60 60C 60C 60C
30sec 30sec 45sec 45sec 45sec 30sec 30sec 45sec 45sec 45sec