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					APPLICATION NOTE




                                              Analysis of Apple Juice
                                                                                                                                                                  7.6
mV                                                                                                                                                                32'
                                                                                                                       Orange juice 1 to 5 dilution               Pr
1100                                                                                                                                                              o


1000

                                                                                                                         10
900                                                                                                                      0.0
                                                                                                                         07'
                                                                                                                         Ar
800                                                                                                                      g


700


600    14.
       34
       5'
500    As
                        23.                                                          73.
       p
                        62                                                           13
400                     1'                                                           1'
                        As                                                           g-
                        n                                                            AB
300                                                                                  A
                  20.
                  88
200               3'                   35.
                  Se                   40
                  r                    7'
100                                    Ala
                                                                                                              94.
                                                                                                              14
0                                                                                                         92. 0' 95.
                          24.                                                                             20 NH 90
                                     34.                                                                  7' 4 1'
             19.          79                                              59.
-100                                 12                                                    75.         90.Ly      EO
             20           0'                      41.   49. 52.     55.   12
                                     2'                    50.                             19          57 s       HN
             3'           Gl                      27    42 55       73    5'
                                     Gly                   99                              2'          4'         H2
             Thr          u                       3'    6'     0'   4'    Ph
-200                                                       9'                              His         Or
                                                  Val   Me Le       Tyr   e
                                                           Ile                                         n
                                                        t      u
-300


-400         20                 30           40         50                 60   70               80   90                100                110        120   130         min




Apple juice has a notable amino acid profile, especially the
concentration of asparagines is high.


Operation Data:


Instrument                                                                      Amino Acid Analyzer ARACUS

Elution Buffers                                                                 Membrapure Ready-to-Use Buffers A, B, C, D, E and F
Reagent                                                                         Membrapure “Reagent for Derivatization”
Pre-Column                                                                      narrow bore, stainless steel, 30 mm
Column                                                                          narrow-bore, stainless steel, 125 mm, ID 3 mm
Method of Detection                                                             Postcolumn-derivatization with Ninhydrin
                                                                                570 nm and 440 nm for detection of Proline and
Detection wavelength
                                                                                Hydroxyproline

Sample                                                                          Apple Juice
Sample Preparation                                                              Membrapure Sample Preparation Kit
Data Acquisition & Analysis Software                                            iPeak

				
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posted:8/30/2011
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Description: The irony is that crazy to go to the gym is to the muscle tear. After you harvest, when your body starts to repair, but over-restored. Go to the gym before the drink 12 - 16 ounces of apple juice can provide 45-60 grams of carbohydrates and quickly give you added energy (because the juice of glucose), and a steady stream of energy (because it also contains fructose). This is to help suppress cortisol and reduce muscle damage, so you can train more groups.