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Allegato B2 Quadro degli obiettivi formativi specifici e delle propedeuticità Corso di Laurea magistrale in Biotecnologie delle piante e degli animali Rau, art. 12, comma 2, lettera b N. Insegnamento Settore Obiettivi formativi specifici Propedeuticità SSD obbligatorie* Plant biotechnology AGR/07 Aim of the course are the following: Genome analysis 1 - to illustrate the organization of DNA in plants with and particular emphasis to transposable elements, repetitive bioinformatics sequences and differential nucleotide composition; - to present the most valuable molecular markers in functional genomics; - to analyze at molecular level the different factors involved in the regulation of gene expression in plants; - to explain recombinant DNA technology and gene transfer in plants; - to show principles and practices in plant cell, tissue and organ culture; - to give students hands-on experience and training in representative plant tissue culture- and genetic engineering techniques; - to acquaint students with experimental design and analysis of plant biotechnology experiments; - to outline the issues and challenges encountered in the area of plant biotechnology. Molecular nutrition AGR/17 The course aims to survey different applications of 2 of plant and animal biotechnology and genetic engineering in the field of II animal nutrition. In particular the course will investigate the integration of genomics into animal sciences, using a system biology approach with the special cases of nutritional sciences. At this regard, the complexity of genome responses to nutritional exposures is investigated with particular focus on transcriptome modifications (nutrigenomics and pharmacogenomics) after specific nutrient administration (antioxidants, CLA, PUFA…). Special cases of bioactive compounds, as those deriving from plant secondary metabolites, in the regulation of gene expressions and protein activity are included in the programme. The interaction of nutrients on epigenetic regulations in animal genomes is also reported. Notes on bioinformatics and tools for the analysis of gene-nutrient interaction will be also provided Plant-pathogen AGR/12 The students will develop a comprehension of the Plant Molecular 3 interaction molecular mechanisms underlying the interaction of Biology; protein fungal and bacterial pathogens with their host. They will Structure and be able to recognize the molecular signals that function characterize plant diseases, the major physiological and genetic aspects of pathogenicity and virulence in the microrganisms, the different aspects of innate immunity and resistance in the plant. Within the general contexts of evolution, environmental fitness and competition among organisms, the most recent research issues with potential for disease control will be acquired. Protein Structure BIO/04 The aim of this course is to cover the structures of 4 and Function proteins and their biophysical chemistry. This topic is intended to show the general principles and the main techniques that allow the identification, isolation and purification of some common proteins. The basic concepts, previously acquired by the student, are developed to examine molecular and physiological aspects of protein purification, folding, distribution into the cell, modification and evolution. The course is integrated by laboratory practical and computer-assisted analysis of protein databases, sequence alignments and 3D structures. Molecular nutrition AGR/13 The students will learn basic and applied concepts of 5 of plant and animal I plant nutrition; in particular mechanisms and regulatory principles of nutrient uptake and plant response to abiotic stresses will be elucidated considering approaches from biochemistry and molecular biology; the role of secondary metabolites in plant adaptation to adverse conditions will also be considered. Applied objectives are the identification of processes contributing to nutrient efficiency and stress tolerance in crop plants, and possible improvements by biotechnological approaches. Genetic Resources AGR/03 6 Biotechnology and IUS/03 genetics legislation 7 Biotechnology in VET/10 The aims of the course is to provide the essential none 8 Animal Reproduction knowledge for understanding the biology of reproduction of domestic animals; in particular, the techniques for synchronization and superovulation of the donor and recipient females, in vivo and in vitro gametes and embryo collection, in vivo and in vitro oocyte maturation and fertilization, and for embryo transfer in domestic and laboratory animals will be described. Technical knowledge will be also provided for oocyte nuclear transfer (cloning), for obtaining transgenic animals and their possible use in animal production and biomedicine, for the embryo and sperm sexing, for the cryopreservation of gametes and embryo, the cryobanking of the genetic resource, and the principal pathologies due to the application of biotechnologies of reproduction (epigenetic defects). Laboratory activity on rabbit superovulation and embryo collection and evaluation will be also organized. Genome analysis BIO/10 9 and bioinformatics Food Safety VET/04 This Program is designed to provide a strong technical 10 education in the areas of food safety of animal product and food inspection with the aim to prepare students for employment in the food industry and regulatory agencies. Students will be educated on analytical and molecular skills applied for foodborne pathogens identification and characterization AND for biomarkers / DNA investigation of species substitution to prevent economic frauds and to assure food traceability. Animal physiology VET/02 The nervous system : Conduction. Development, 11 and welfare maturation. The spinal cord and spinal nerve. Reflexes. The brain, brainstem, cranial nerves, cerebellum, diencephalon, cerebellum. The autonomy nervous system. The endocrine system: The hormones. Transport, metabolism, and secretion. Receptors. The hypothalamus, pituitary gland ,melatonin, thyroid gland, adrenal glands. Brain modulator: Biological rhythms, circadian rhythms. Cyclical changes over longer intervals. The skin: epidermis, dermis hypodermis the skin surface, hair and feathers, gland endocrine functions. The senses: Sensory cells. Sensory perception. The sense of touch, pressure, temperature, pain, smell, taste, vomeronasal organ. Hearing, echolocation, balance, magneto reception, vision. Thermoregulation: core and skin temperature, production and loss of the temperature, the thermo neutral zone, the adaptation. Stress: classification. The axis of stress : nervous, endocrine and coetaneous. Livestock production AGR/18 Livestock production course covers the aspects of farm 12 (mod. Livestock animal feeding and husbandry, in relation to the production I) environmental, social and economical issues, offering to the students a general frame for the applications of biotechnology. After an introduction of feedstuffs compositions, the course offers the knowledge of animal nutrition, feeding and ration formulations. The course continues with the description of milk production systems of ruminants and meat production systems of ruminants and pigs, giving an insight of the optimum feeding and management techniques to improve animal products, animal welfare and to ensure environmental sustainability of farms through a rational utilization of natural resources. Methods to improved animal production systems are also reported, indicating possible modification needed to be developed to respond to increasing human populations and to take advantage of improvements in the genetic potential of animals. The course is completed with processing of animal products, with the aim to develop an efficient milk and meat industry, to reduce animal and human health risks, through enhancing hygiene and reducing of wastes. Special cases of production systems for laboratory animals are also offered Livestock production AGR/20 The course is aimed at providing the students with the (mod. Livestock basic knowledge of poultry and aquatic animals farming production II) systems. It first introduces, under a comparative perspective, to the main morpho-functional traits and environmental needs of poultry, fishes, shrimps and shellfish. Secondly it describes major clues of their nutrition, feeding, reproduction, genetic improvement, with an updated vision on technologies and management practices to improve productivity, animal welfare and environmental sustainability in farming broiler chicken, laying hens, marine and freshwater fish, shrimps and shellfish. The last part of the course dealth with the quality and safety of poultry and aquaculture food products, with particular emphasis to the methods and systems to improve their nutritional quality, ensuring their traceability and safety along the supply-chain. Major current biotechnological applications in the poultry and aquaculture industry are also given. Plant Cell Physiology BIO/04 The purpose of this course is to provide an initial focus on 14 and Tissue culture the main metabolic pathways in relation to their regulatory mechanisms. Then, the synthesis of the oligosaccharides, polysaccharides, storage lipids and proteins will be described. This argument ends with the description of secondary metabolites (terpenes, phenols and alkaloids). The second part provides information on plant tissue culture, propagation techniques and crop improvement techniques. The course is integrated by practical teaching on the main topics. 15 Plant breeding (mod. AGR/07 Aim of this course is to explain the principles underlying Plant Principles of Crop conventional plant breeding, to illustrate the basic biotechnology breeding) methods used to select new varieties in both autogamous and allogamous species and to present new tools for plant breeding offered by advances in cell and tissue culture techniques and in the “omic” disciplines. The following topics will also be illustrated and discussed: definition and aims of plant breeding; plant breeding and sustainable agriculture; plant breeding and food insecurity; origin-, diversification- and spreading centres of cultivated species; plant domestication; gene pools and germplasm collections; role of mutation, interspecific hybridization and polyploidization in the evolution of plant species; mating systems, sterility and self-incompatibility. The course will consist of lectures. Plant Breeding AGR/03 The objective of this course is to illustrate the Plant (mod. Tree Genetics conventional methods used to select new varieties in biotechnology and Breeding) vegetatively propagated crops with particular emphasis to fruit and timber trees, as well as the most advanced techniques used to improve selection efficiency in wooden species. Aspects related to fruit tree breeding, including polyploid inheritance, apomixis and genetic analysis of complex loci will be presented and discussed. The course will consist of lectures, practical exercises and visits to ongoing field trials.
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