M ean Fluorescence (M FI)
A100
80
40 20 0 100 101 102 103
91.3 101 102 103 104 VK-8 (FITC)
25
0
0
1/MFI 1/MFI
60
50 nM 100 43.75 nM 37.5 nM 80 31.25 nM 25 nM 60 18.75 nM 40 12.5 nM 9.38 nM 20 6.25 nM 0 3.125 nM 100 1.25 nM 0.625 nM No meso-Fc
% of Max % of Max
100
75
0.100
% of Max
50
0.075
0.050
0.025
0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75
1/[Meso-Fc] 1/[Meso-Fc]
10
20
30
40
50
60
Meso-Fc (nM)
B 100
80
GAR-FITC
104
120
60 40 20 0 0 10 101 102
60.5
~10 s 5 min 10 min 20 min 30 min 40 min 50 min 60 min GAR 104
Mean Fluorescence
100 80
100 80 60 40 20 0 0 10 20 30 40 50 60 70
% of Max
% of Max
60 40 20 0 0 10 101 102 103 104
VK-8 (FITC)
Time (mins)
103
% of Max
80 % of Max 60 40 20 0 100 101 102 103 GAR-FITC 104
100 nM 100 60 nM 80 40 nM 60 20 nM 40 10 nM 20 6 nM 4 nM 0 100 101 102 103 2 nM VK-8 (FITC) 20 only No MUC16
M ean Fluorescence
104
C 100
GAR-FITC
175
150
125
100
75
50
25
0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
MUC16:Meso-Fc