EUROPEAN JOURNAL OF INFLAMMATION
Vol. 7, no. 2, 69-77 (2009)
NO EVIDENCE OF PATHOLOGICAL AUTOIMMUNITY FOLLOWING MYCOBACTERIUM LEPRAE HEAT-SHOCK PROTEIN 65-DNA VACCINATION IN MICE
D.S. LIMA, C.R. ZÁRATE-BLADÉS, P.R.M. SOUZA, A.P. TROMBONE, R.R. SANTOS-JUNIOR1, I.T. BRANDÃO, A.P. MASSON, V.L. BONATO, A.A.M. COELHO-CASTELO, A. SARTORI2, M. VENDRAMINI3, E.G. SOARES4, L.A. BENVENUTTI3, C.L. SILVA and V. COELHO5,6
The Centre for Tuberculosis Research, Department of Biochemistry and Immunology, Medicine School of Ribeirao Preto, University of São Paulo, São Paulo; 1Department of Clinical Analyses, Faculty of Pharmaceutical Sciences, São Paulo State University, Araraquara, São Paulo; 2Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University, Botucatu, São Paulo; 3Department of Pathology, University of São Paulo Medical School, São Paulo; 4Department of Pathology, School of Medicine of Ribeirão Preto, University of São Paulo,Ribeirão Preto, São Paulo; 5Heart Institute (InCor), University of São Paulo Medical School, São Paulo; 6Institute for Investigation in Immunology, National Institute of Science and Technology - INCT, São Paulo, Brazil
Mailing address: Verônica Coelho, Instituto do Coração, Laboratório de Imunologia, Faculdade de Medicina da Universidade de São Paulo, 05403-001 São Paulo, SP, Brasil, Tel: ++55 11 30695905 Fax: ++55 11 30829350, e-mail: vecoelho@usp.br
Figure S-9: Histological analysis of the skeletal muscle. Microphotographs of the skeletal muscle showing no alteration of the normal structure in all groups of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 20x objective.
Figure S-10: Histological analysis of the eye. Microphotographs of the eye showing no alteration of the normal structure in all groups of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 10x objective.
Figure S-11: Histological analysis of the skin. Microphotographs of the skin showing no alteration of the normal structure in all groups of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 10x objective.
Figure S-12: Histological analysis of the intestine. Microphotographs of the intestine showing no alteration of the normal structure in all groups of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 20x objective.
Figure S-13: Histological analysis of the lung. Microphotographs of the lung showing only minor alterations of the normal structure in all groups of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 10x objective.
Figure S-14: Histological analysis of the spleen. Microphotographs of the spleen showing no alteration of the normal structure in all groups of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 10x objective.
Figure S-15: Histological analysis of the thymus. Microphotographs of the thymus showing no alteration of the normal structure in all groups of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 10x objective.
Figure S-16: Histological analysis of the lymph node. Microphotographs of the lymph node showing no alteration of the normal structure in all gropes of animals at all three experimental times analysed (45, 75 and 210 days following the first dose of the DNAhsp65 vaccine). The samples were stained with hematoxylin and eosin. All of the pictures were taken with a 10x objective.