PCR Brochure v.final by AttFarrell

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									Premium PCR Enzymes
    Takara Ex Taq ™ DNA Polymerase
    Applications
     • High sensitivity, high yield PCR, even with DNA                             λ DNA                       ≤30 kb
       from problem organisms and samples                                          Genomic DNA                 ≤20 kb
                                                                                   Accuracy vs. Taq             4.5X
     • PCR requiring less optimization and increased                               Hot-start version              √
       reproducibility                                                             3'-A ends                      √
     • Standard to long DNA amplifications                                         Optimized Buffer               √
                                                                                   Premix available               √
     • Specific, sensitive real-time PCR with a wide
       dynamic range

    Description
    Takara’s Ex Taq™ DNA Polymerase combines the proven perform-
    ance of Taq DNA Polymerase with an efficient 3’ → 5’ exonuclease                    Call or
                                                                                                 v
    activity for unsurpassed PCR performance. In routine PCR applica-                  website isit our
    tions, the Ex Taq™ Enzyme Buffer System results in higher yields,                           for a
    lower error rates (approximately 4.5X lower, as determined by the                     Sample Free
    Kunkel method), and more reproducible results than standard Taq                                 !
    DNA Polymerase. This system also allows amplification of longer
    products than Taq DNA Polymerase, with 20 kb lengths possible from
    genomic DNA and up to 30 kb possible from λ DNA.
    PCR products generated with Ex Taq™ contain a mixture of 3′-A
    overhangs and blunt ends. Generally, >80% cloning efficiency is
    achieved into T-vectors.
    The high performance of Ex Taq™ makes it well suited for many
    applications. Standard, Hot-start, Premix, and Real-Time PCR
    (qPCR) versions are available.



    Robust Amplification

    Ex Taq™: High Sensitivity and High Yield
    Ex Taq: DNA Amplification from Problem Organisms and Sources


    Takara’s Ex Taq™ DNA Polymerase’s proven performance makes it
    an excellent candidate for routine PCR. The robust Ex Taq™
    enzyme-buffer system results in high product yields from even very
    small amounts of starting DNA (0.025 pg). This system has also
    allowed researchers to amplify DNA from problem organisms and
    sources, including high polyfaccharide plants, algae, and human
    biopsy and fecal specimens.


■   This figure illustrates amplification of a 6 kb target from E. coli
    genomic DNA with Takara Taq and Ex Taq™ DNA Polymerases. PCR
    was performed using the indicated amounts of template, with either
    Takara Taq™ (Lanes T1-T4) or Ex Taq™ (Lanes E1-E4). Each 50 µL
    reaction contained 0.9 units of enzyme. Lanes M contain λ Hind III            Amplification of a 6 kb target from E. coli
    DNA Markers. The Ex Taq™ lanes show superior amplification over                 genomic DNA with Takara Taq™ and
    Taq DNA Polymerase.                                                                 Ex Taq™ DNA Polymerases.




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          Takara Mirus Bio • 505 S. Rosa Road, Suite 101, Madison WI, 53719 • 888-251-6618 • www.takaramirusbio.com
    Increased Reproducibility and Minimal Optimization in Standard PCR

    Ex Taq™ and Ex Taq™ Hot Start: Reliable, Consistent PCR


    Takara's Ex Taq™ DNA Polymerase and Ex Taq™ Hot Start DNA
    Polymerase include dynamic enzyme-buffer systems which result in
    reliable, consistent PCR performance for even challenging samples,
    and often with less time spent on optimization. The system is so
    robust that, in many applications, the amount of Ex Taq™ and Ex
    Taq™ Hot Start required is less than one-half of the enzyme amount
    necessary with conventional Taq DNA Polymerase

                                                                         1      2   3     4   5    6    7   8    9   10   11   12
■   This figure illustrates amplification of a 1.1 kb Bacillus
    sp. genomic DNA target using Ex Taq™ Hot Start DNA
    Polymerase vs. several competing enzymes. Lanes 1
    and 2, Ex Taq™ Hot Start with 10X Ex Taq™ buffer;
    lanes 3 and 4, AmpliTaq Gold® with supplied buffer;
    lanes 5 and 6, AmpliTaq Gold® with 10X AmpliTaq Gold®
    Buffer; lanes 7 and 8, Advantage™ 2 Polymerase; lanes
    9 and 10, Platinum™ Taq DNA Polymerase; and lanes
    11 and 12, Proof-Start™ DNA Polymerase. This experi-
    ment illustrates improved signal intensity and lower
    background with Ex Taq™ Hot Start over competing                   Amplification of a 1.1 kb Bacillus sp. genomic DNA target.
    products.




    Specific, Sensitive Real-Time PCR (qPCR)

    SYBR® Premix Ex Taq™: Superior Specificity and Increased Amplification Efficiency for qPCR


    Takara has a wide range of real-time PCR products, including the
    CycleavePCR Core Kit, Ex Taq™ R-PCR Version 2.1, and the Real
    Time One Step RNA PCR Kit. All of these products contain Ex Taq™
    Hot Start DNA Polymerase, dNTPs, Mg2+, and a newly formulated
    real time buffer which provides superior specificity and increased
    amplification efficiency in real time PCR.
    Takara’s newest qPCR addition, SYBR® Premix Ex Taq™ (Perfect
    Real Time), is a convenient (2X) premix which contains SYBR®
    Green I, and a separate tube of ROX reference dye. It is compatible
    with all major qPCR instruments, including the Smart Cycler®,
    LightCycler®, ABI 7000,7300, 7500 and 7700, RotorGene™, as well
    as other real time instruments. It can detect as few as 100 copies of
    starting template, and possesses a dynamic range of 7-8 orders of
    magnitude using a λ DNA template. Excellent standard curves for
    various real time instruments have been established with this
    enzyme. SYBR® Premix Ex Taq™ can be used for rapid reactions
    because it provides conditions favorable for short denaturation steps
    and is suitable for shuttle PCR.

                                                                                        Amplification curve and Melting curve for
■   This figure shows amplification and melting curves using SYBR®                      SYBR Premix Ex Taq™ (Perfect Real Time)
    Premix Ex Taq™ (Perfect Real Time) on a Cepheid Smart Cycler®.                         using the Smart Cycler® (Cepheid)
    The specificity and sensitivity of this system is illustrated by the wide
    dynamic range and sharp melting curve.


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           Takara Mirus Bio • 505 S. Rosa Road, Suite 101, Madison WI, 53719 • 888-251-6618 • www.takaramirusbio.com
                                                                  Guide to Takara P
                                                Product Size          Product size
 Polymerase*                 Amplification         λ DNA          Human Genomic DNA       Fidelity**    Proofreading      Specificity
                              Efficiency     Recommended/Max       Recommended/Max                         Activity

 Ex Taq™                         ++++          20 kb/30 kb            10 kb/20 kb         4.5 X Taq         Yes                ++

 Premix Ex Taq™                  ++++          20 kb/30 kb            10 kb/20 kb         4.5 X Taq         Yes                ++

 Ex Taq™ HS                      ++++          20 kb/30 kb            10 kb/20 kb         4.5 X Taq         Yes             ++++

 Ex Taq™ R-PCR                   ++++               _                     _               4.5 X Taq         Yes             ++++

 Premix Ex Taq™
 (Perfect Real Time)             ++++               _                     _               4.5 X Taq         Yes             ++++

 SYBR Premix Ex Taq™
 (Perfect Real Time)             ++++               _                     _               4.5 X Taq         Yes             ++++

 LA Taq™                         +++           35 kb/48 kb            20 kb/30 kb         6.5 X Taq         Yes                ++

 LA Taq™ w/GC Buffer             +++           35 kb/48 kb§         (20 kb/30 kb)§      (6.5 X Taq)‡        Yes                ++

 LA PCR Kit                      +++           35 kb/48 kb            20 kb/30 kb         6.5 X Taq         Yes                ++

 One-Shot LA PCR Mix             +++           35 kb/48 kb            20 kb/30 kb         6.5 X Taq         Yes                ++

 LA Taq™ HS                      +++           35 kb/48 kb            20 kb/30 kb         6.5 X Taq         Yes             ++++

 Z-Taq™                          +++           20 kb/30 kb            10 kb/20 kb          3 X Taq          Yes                ++

 Taq                              ++            6 kb/12 kb             2 kb/4 kb           1 X Taq           No                ++

 Premix Taq                       ++            6 kb/12 kb             2 kb/4 kb           1 X Taq           No                  +

 Taq HS                           ++            6 kb/12 kb             2 kb/4 kb           1 X Taq           No             ++++



All of Takara’s premium PCR enzymes utilize Long and              Taq Polymerase (determined by the Kunkel method), and
Accurate (LA) PCR technology, which uses a mixture of             the perfected enzyme-buffer system often results in less
Taq Polymerase and a proofreading polymerase to gener-            time spent on optimization than other long PCR polymeras-
ate products with improved fidelity and extended length. By       es. Standard, Hot-Start, Premix, and GC-Rich Versions of
additionally optimizing buffer and reaction conditions,           LA Taq™ are available.
Takara has produced a line of high-performance poly-
                                                                  Both Ex Taq™ DNA Polymerase and LA Taq™ DNA
merases and products for specialized applications.
                                                                  Polymerase offer excellent performance with DNA templates
Takara Ex Taq™ DNA Polymerase is optimized for maximal            > 5 kb in length. Ex Taq™ can amplify fragments up to 20 kb
performance in both routine and challenging PCR. It offers        using a genomic DNA template and up to 30 kb with λ DNA.
high sensitivity, increased product yield and length, and         When amplifying products from difficult templates, such as
improved reproducibility and fidelity over Taq Polymerase.        plant samples or “dirty” specimens, Ex Taq™ is generally the
Standard, Hot-Start, Premix, and Real-Time PCR (qPCR)             enzyme of choice. LA Taq™ is the best choice when working
versions of Ex Taq™ are available.                                with very long templates (>15 kb), or templates with second-
                                                                  ary structure or high GC content.
Takara’s LA Taq™ DNA Polymerase is optimized specifically
for long PCR, and can be used to synthesize products up to        Both Ex Taq™ DNA Polymerase and LA Taq™ DNA
48 kb in length. Fidelity with LA Taq™ is 6.5X better than        Polymerase are available in Premix versions, which consist


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          Takara Mirus Bio • 505 S. Rosa Road, Suite 101, Madison WI, 53719 • 888-251-6618 • www.takaramirusbio.com
PCR Polymerases
                                                                                                             Guidelines for            Terminal
 Convenience      GC-Rich       Hot-Start PCR     Real Time PCR         Low DNA            Processing          Length of         Transferase Activity
                 Templates                           (QPCR)              Enzyme              Speed              Primers            (3’-A overhang)

     ++               +               _                 _               ≤ 10 fg            1-2 kb/min           20-30 bp                  Yes

    ++++              +               _                 _               ≤ 10 fg            1-2 kb/min           20-30 bp                  Yes

     ++               +             ++++                _               ≤ 10 fg            1-2 kb/min           20-30 bp                  Yes

     ++               +             ++++               ++++             ≤ 10 fg                 _                    _                    Yes



    ++++              +             ++++               ++++             ≤ 10 fg                 _               17-25 bp                  Yes



    ++++              +             ++++               ++++             ≤ 10 fg                 _               17-25 bp                  Yes

     ++               +               _                 _               ≤ 10 fg            1-2 kb/min           25-35 bp                 Yes+

     ++             ++++              _                 _               ≤ 10 fg            1-2 kb/min           25-35 bp                 Yes+

     ++             ++++              _                 _               ≤ 10 fg            1-2 kb/min           25-35 bp                  Yes+

    ++++              +               _                 _               ≤ 10 fg            1-2 kb/min           25-35 bp                  Yes+

     ++               +             ++++                _               ≤ 10 fg            1-2 kb/min           25-35 bp                  Yes+

     ++              ++               _                 _               ≤ 10 fg            4-5 kb/min                _                    Yes

     ++               +               _                 _               ≤ 10 fg             1 kb/min                 _                    Yes

    ++++              +               _                 _               ≤ 10 fg             1 kb/min                 _                    Yes

     ++               +             ++++                _               ≤ 10 fg             1 kb/min                 _                    Yes



           of a single solution containing enzyme, buffer, Mg2+, and          with separate components, or as Premix versions both
           dNTPs, and provide the convenience of simple reaction              with and without SYBR Green I. Takara’s latest addition to
           assembly. Thus, the number of pipetting steps is reduced,          the qPCR product line, SYBR Premix Ex Taq™ (Perfect
           saving time and labor, and limiting opportunities for con-         Real Time), additionally includes a separate tube of ROX
           tamination.                                                        reference dye.
           Hot-start versions of Ex Taq™ DNA Polymerase and LA
           Taq™ DNA Polymerase, which contain a monoclonal Taq                 * All of Takara’s PCR polymerases are provided with dNTPs
           antibody, are also available. These hot-start version                 and buffer.
           enzymes provide increased specificity and sensitivity,              + T-vector cloning efficiency diminishes as the length of the
           reduce background, and allow room-temperature reaction                PCR product to be cloned increases above 5 kb.
           assembly.                                                           § When used with GC Buffer I.
                                                                               ‡ When amplifying GC-rich templates, the fidelity is reduced.
           Real-Time PCR (qPCR) versions of Ex Taq™ DNA
                                                                               ** All fidelity determined by using the Kunkel method.
           Polymerase are also available for high performance
           qPCR. Ex Taq™ Hot-Start Polymerase is supplied in                   Takara Bio owns the worldwide rights to LA PCR technology, and also pro-
           these versions with optimized high-specificity qPCR                 vides a wide selection of PCR Kits, including kits for RT-PCR,
                                                                               RACE, Competitive PCR, PCR cloning, PCR mutagenesis, and
           buffers. Takara’s real time products are available as Kits          organism and gene screening.



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                  Takara Mirus Bio • 505 S. Rosa Road, Suite 101, Madison WI, 53719 • 888-251-6618 • www.takaramirusbio.com
    Takara LA Taq ™ DNA Polymerase
    Applications
                                                                                     λ DNA                    ≤48 kb
     • Long PCR (up to 48 kb)                                                        Genomic DNA              ≤30 kb
     • PCR amplification of difficult templates due to                               Accuracy vs. Taq          6.5X
       secondary structure or high GC content                                        3'-A ends                   √
                                                                                     Hot-start version           √
     • Increased specificity and reduced background using                            Optimized Buffer            √
       Hot-Start technology                                                          Premix available            √
                                                                                     G-Rich Version
    Description                                                                      available                    √
    Takara’s LA Taq™ DNA Polymerase is composed of a mixture of Taq
    Polymerase with a proofreading polymerase which, when used with
    Takara’s specially optimized LA Buffer II, provides PCR products of
    maximum length (≤ 30 kb for genomic DNA, ≤ 48 kb for λ DNA) with sig-
    nificantly improved fidelity. Because of the presence of the proofreading               Call or
    polymerase, the fidelity of LA Taq™ is 6.5X better than Taq Polymerase              website visit our
    alone (determined by the Kunkel Method). Furthermore, many users                              to
    note that this high-performance system requires less optimization than               trial siz order your
                                                                                                  e of LA
    other long DNA polymerases. LA Taq™ DNA Polymerase has been                                           Taq
    used by researchers with a variety of templates including smooth mus-
    cle phosphoprotein, Ascidians (sea squirts), Drosophila, Arabidopsis, C.
    elegans, and the root parasite, Orobanche cumana. For templates with
    high secondary structure or high GC content, LA Taq™ DNA
    Polymerase with GC Buffers provides two different buffers which can be
    used to destabilize problem hairpin loops and synthesize templates with
    up to 72% GC content.
    PCR products generated with LA Taq™ contain a mixture of 3’-A over-
    hang and blunt ends. Generally, >80% cloning efficiency is achieved into
    T-vectors.
    Standard, GC-Rich, Premix, Hot-Start, and Kit versions of LA Taq™ are
    available.


     Long Amplification and Excellent Fidelity

    LA Taq™: Amplification of 5-15 kb fragments; extensions to 48 kb possible
    LA Taq™: Long PCR from limited amounts of starting template

    Takara’s LA Taq™ DNA Polymerase overcomes traditional problems asso-
    ciated with efficient amplification of DNA fragments ≥5 kb through an opti-
    mized enzyme-buffer system. This system incorporates LA (long and accu-
    rate) PCR technology, and results in increased product fidelity, sensitivity,
    and length. With LA Taq™, routine PCR amplification of templates ranging
    from 5-20 kb can be expected, with lengths of up to 48 kb possible on
    some templates. This reliable system also allows amplification from small-
    er starting template amounts than other long PCR systems, making it the
    enzyme of choice for synthesis of long genomic DNA fragments.


■   The sensitivity of LA Taq™ is demonstrated in this figure, which shows
    good amplification efficiency of a 21.5 kb human genomic DNA fragment                Amplification efficiency of a 21.5 kb
    with various amounts of template. Lane 1 = 500 ng; Lane 2 = 50 ng; Lane             genomic DNA fragment using Takara
    3 = 5 ng. Lane M = λ Hind III MW marker.                                            LA Taq™ was compared utilizing vari-
                                                                                        ous amounts of human genomic DNA
                                                                                                      template.


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           Takara Mirus Bio • 505 S. Rosa Road, Suite 101, Madison WI, 53719 • 888-251-6618 • www.takaramirusbio.com
     Amplification of GC-Rich Templates

    LA Taq™ with GC buffers: Amplification of templates with high
    GC content or secondary structure


    Takara’s LA Taq™ DNA Polymerase with GC Buffers includes two dif-
    ferent specialized buffers (GC Buffer I and II) which can be used to
    facilitate amplification of templates with high GC content and/or sec-
    ondary structure. These buffers were developed and optimized for
    use with LA Taq™ to address polymerase slippage on GC-rich tem-
    plates and amplification problems associated with hairpin loops and
    other DNA secondary structures. Users are encouraged to test both
    buffers to see which formulation provides the best results with their
    template. GC Buffer I and II are also supplied as part of the compre-
    hensive LA PCR Amplification Kit, which additionally contains Mg2+-
    plus and Mg2+-minus LA Buffer II, 25 mM MgCl2, Control DNA, and
    MW markers.

■   This figure demonstrates the performance of the LA Taq™ DNA                      Amplification of a portion of
    Polymerase with GC Buffers in amplification of a portion of the                Huntington's Disease (IT 15 CAG
    Huntington’s Disease gene (IT 15 CAG repeat). Two DNA fragments                repeat) using LA Taq™ with GC
    were amplified using LA Taq™ with either LA Buffer II (lane 1), GC                         Buffers.
    Buffer I (lane 2), or GC Buffer II (lane 3). The GC content of the 262
    bp fragment is 73%; the GC content of the 358 bp product is 71.5%.
    a GC Kit from company A is in lane 4. Lane M contains a 100 bp
    molecular weight ladder.




     Sensitive, Specific Long PCR

    LA Taq™ Hot Start: Facilitates room temperature assembly

                                                                                             1     2      3
    LA Taq™ Hot-Start DNA Polymerase consists of LA Taq™ DNA
    Polymerase plus a monoclonal Taq antibody bound to the poly-
    merase. It retains all of the high performance features of LA Taq™,
    and because the enzyme is sequestered by the antibody until the first
    denaturation step, it additionally provides increased reaction specifici-
    ty and reduced background.


■   This figure shows amplification of a long (15 kb) λ DNA fragment
    using either LA Taq™ (lane 2) or LA Taq™ Hot-Start (lane 3) DNA
    Polymerase. Use of LA Taq™ Hot-Start prevented the non-specific
    product amplification observed with LA Taq™.
    In addition, room temperature reaction assembly, (which is especially
    important in automation), is possible with this formulation.                     Amplification of a 15 kb λ DNA
                                                                                     fragment using LA Taq™ DNA
                                                                                      Polymerase (Lane 2) and LA
                                                                                      Taq™ DNA Polymerase, Hot
                                                                                     Start Version (Lane 3). Lane 1
                                                                                    contains a λ Hind III MW marker.




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           Takara Mirus Bio • 505 S. Rosa Road, Suite 101, Madison WI, 53719 • 888-251-6618 • www.takaramirusbio.com
Application                                       Recommended Product
Efficient and Sensitive PCR                       Ex Taq™ DNA Polymerase, Ex Taq™ Hot Start DNA Polymerase
Long and Accurate PCR                             LA Taq™ DNA Polymerase, LA Taq™ Hot Start DNA Polymerase
High Fidelity PCR                                 LA Taq™ DNA Polymerase, Ex Taq™ DNA Polymerase
Hot-Start PCR                                     Ex Taq™ Hot Start DNA Polymerase, LA Taq™ Hot Start DNA Polymerase
Real-Time PCR                                     SYBR® Premix Ex Taq™ with ROX, Ex Taq™ R-PCR DNA Polymerase, Premix Ex Taq™ (Perfect Real Time)
Automated PCR                                     Ex Taq™ Premix, Taq Premix, One Shot LA Taq ™ Mix, Premix Ex Taq™ (Perfect Real Time)
Difficult templates                               Ex Taq™ DNA Polymerase, Ex Taq™ Hot Start DNA Polymerase
GC-rich or High Secondary Structure               LA Taq™ DNA Polymerase with GC Buffers, LA PCR Kit
General PCR                                       Taq Polymerase, Ex Taq™ DNA Polymerase




       Ordering Information
       Ex Taq™ Products
         Product No.                      Description                                                             Quantity                               Price
         TAK RR001A                       Ex Taq™ DNA Polymerase                                                  250 units (200 reactions)              $    158.00
         TAK RR001B                       Ex Taq™ DNA Polymerase                                                  1,000 units (800 reactions)            $    567.00
         TAK RR001C                       Ex Taq™ DNA Polymerase                                                  3,000 units (2400 reactions)           $   1483.00
         TAK RR01AM                       Ex Taq™ DNA Polymerase (Mg2+-free Buffer)                               250 units (200 reactions)              $    158.00
         TAK RR01BM                       Ex Taq™ DNA Polymerase (Mg2+-free Buffer)                               1,000 units (800 reactions)            $    567.00
         TAK RR01CM                       Ex Taq™ DNA Polymerase (Mg2+-free Buffer)                               3,000 units (2400 reactions)           $   1483.00
         TAK RR003                        Ex Taq™ DNA Polymerase Premix                                           500 µl x 6 (120 reactions)             $    147.00
         TAK RR006A                       Ex Taq™ Hot Start DNA Polymerase                                        250 units (200 reactions)              $    189.00
         TAK RR006B                       Ex Taq™ Hot Start DNA DNA Polymerase                                    1,000 units (800 reactions)            $    666.00
         TAK RR031A                       Ex Taq™ R-PCR Enzyme-Buffer system, Version 2.1                         250 units (200 reactions)              $    189.00
         TAK RR031B                       Ex Taq™ R-PCR Enzyme-Buffer system, Version 2.1                         1,000 units(800 reactions)             $    666.00
         TAK RR039A                       Premix Ex Taq™ (Perfect Real Time) DNA polymerase                       200 reactions                          $    219.00
         TAK RR039B                       Premix Ex Taq™ (Perfect Real Time) DNA polymerase                       400 reactions                          $    437.00
         TAK RR041A                       SYBR® Premix Ex Taq™ (Perfect Real Time) with ROX                       200 reactions                          $    273.00
         TAK RR041B                       SYBR® Premix Ex Taq™ (Perfect Real Time) with ROX                       400 reactions                          $    534.00


       LA Taq™ Products
         Product No.                      Description                                                             Quantity                               Price
         TAK RR002T                       LA Taq™ DNA Polymerase (Trial Size)                                     50 reactions                           $     65.00
         TAK RR002M                       LA Taq™ DNA™ Polymerase                                                 250 units (100 reactions)              $    263.00
         TAK RR002B                       LA Taq™ DNA Polymerase                                                  1000 units (400 reactions)             $    979.00
         TAK RR002C                       LA Taq™ DNA Polymerase                                                  3,000 units (1200 reactions)           $   2781.00
         TAK RR002A                       LA Taq™ Supplement (with Mg2+-free Buffer)                              125 units (50 reactions)               $    131.00
         TAK RR02AG                       LA Taq™ DNA Polymerase (with GC Buffers)                                125 units (50 reactions)               $    131.00
         TAK RR004                        One Shot LA PCR Mix (with GC Buffers)                                   24 reactions                           $    113.00
         TAK RR013A                       LA PCR Amplification Kit, Version 2.1                                   50 reactions                           $    221.00
         TAK RR013B                       LA PCR Amplification Kit, Version 2.1                                   100 reactions                          $    420.00
         TAK RR042A                       LA Taq™ Hot Start DNA Polymerase                                        125 units (50 reactions)               $    171.00
         TAK RR042B                       LA Taq™ Hot Start DNA Polymerase                                        500 units (200 reactions)              $    631.00
         TAK RR042T                       LA Taq™ Hot Start DNA Polymerase (Trial Size)                           50 units                               $     81.00


       Taq Products
         Product No.                      Description                                                             Quantity                               Price
         TAK R001A                        Taq DNA Polymerase                                                      250 units                              $ 113.00
         TAK R001B                        Taq DNA Polymerase                                                      1,000 units (4 x 250 units)            $ 391.00
         TAK R001C                        Taq DNA Polymerase                                                      3,000 units (12 x 250 units)           $ 1,030.00
         TAK R001AM                       Taq DNA Polymerase (with Mg2+-free Buffer)                              250 units                              $ 113.00
         TAK R001BM                       Taq DNA Polymerase (with Mg2+-free Buffer)                              1,000 units (4 x 250 units)            $ 391.00
         TAK R001CM                       Taq DNA Polymerase (with Mg2+-free Buffer)                              3,000 units (12 x 250 units)           $ 1,030.00
         TAK R004A                        Taq DNA Polymerase Premix                                               120 reactions (6 x 500 µL)             $ 123.00

   Ex Taq™ and LA Taq™ are trademarks of Takara Bio Inc. SYBR® is a registered trademark of Molecular Probes, Inc. Lightcycler® is a registered trademark of a member of
   the Roche group. RotorGene™ is a trademark of Corbett Research. Smart Cycler® is a registered trademark of Cepheid. AmpliTaq® Gold is a trademark of PE Applied
   Biosystems. Advantage™ is a trademark of BD Biosciences Clontech. Platinum® Taq is a trademark of Invitrogen Life Technologies. Proof-Start™ is a trademark of Qiagen,
   Inc. LA PCR technology is covered by U.S. Patent No. 5,436,149 issued to Takara Bio Inc. Takara Bio’s Hot-Start PCR-related products are licensed under U.S. Patent
   5,338,671 and 5,587,287 and corresponding patents in other countries. Takara PCR Related Products are sold under a licensing arrangement with Roche Molecular
   Systems and F. Hoffman La Roche Ltd. and Applied Biosystems.

   Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process (and RT or other as appropriate) in conjunction with
   a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Applied Biosystems or as pur-
   chased, ie., an authorized thermal cycler.

								
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