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Genomic DNA Extraction from Tofu

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					                                                                                                                                                      QuickGene DNA tissue kit S (DT-S)
              DC-3                                                                                                                                    QuickGene SP kit DNA tissue (SP-DT)


         Genomic DNA Extraction from Tofu
             Protocol
                                                         2 ml micro tube
                                                                               Slice of tofu*1 : -80 mg                                                             *1 Tofu, pinched with paper towel
                                                                                                                                                                       over night, is then drained.
                                                                               MDT : 180 µl
                                                                               EDT : 20 µl

             Incubate for over night on Rotary Shaker at 55°C, and dissolve the tofu completely.

                                                                               10,000 rpm, 3 min, RT


                                   Transfer the supernatant to 1.5 ml micro tube*2                                                                                  *2 Oil content floating on
                                                                                                                                                                       supernatant is not.
                                                                               LDT : 180 µl
                                         Mix thoroughly by vortexing for 15 sec
                                                   Flash spin down


                                                 Incubate at 70°C for 10 min
                                                      Flash spin down
                                                                               > 99% ethanol : 240 µl
                                         Mix thoroughly by vortexing for 15 sec
                                                   Flash spin down



                                                                Lysate


                     Transfer all contents of the micro tube into the cartridge of QuickGene


                                 Refer to the extraction protocol of the device used.
                                            QuickGene-810 to p.4-3
                                            QuickGene-Mini80 to p.4-10
                                            QuickGene SP kit to p.4-17


                                         Genomic DNA (Elution volume : 200 µl)


             Results
              Electropherogram
                 M     1     2    3     4    5
                                                        M : marker
                                                        1 : Tofu 5 mg
                                                        2 : Tofu 10 mg
                                                        3 : Tofu 30 mg
                                                        4 : Tofu 50 mg
                                                        5 : Tofu 80 mg




               This book includes some protocol that have been performed, but not yet been approved. Depending on sample and storage conditions, nucleic acid may
3 - IV - 4     not be extractable. Therefore, we cannot guarantee accurate data.
               The extracted nucleic acid contains unintended acid (ex: when extracting DNA, RNA is also extracted).
 The yield of genomic DNA
   Amount of tofu         Yield(ng/µl)
         5 mg                42.81
         10 mg              104.85
         30 mg              254.18
         50 mg              498.0
         80 mg              394.3

 Protein contamination : A260/280
   Amount of tofu          A260/280




                                                                                                                                                                                 DC-3
         5 mg                  1.92
         10 mg                 1.87
         30 mg                 1.93
         50 mg                 2.07
         80 mg                 2.02

 Chaotropic salt contamination : A260/230
   Amount of tofu          A260/230
         5 mg                  1.29
         10 mg                 1.35
         30 mg                 1.98
         50 mg                 2.05
         80 mg                 1.93

 Other
 No Data


Common protocol is usable for the following
 No Data




                    This book includes some protocol that have been performed, but not yet been approved. Depending on sample and storage conditions, nucleic acid may
                    not be extractable. Therefore, we cannot guarantee accurate data.
                    The extracted nucleic acid contains unintended acid (ex: when extracting DNA, RNA is also extracted).
                                                                                                                                                                         3 - IV - 5

				
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posted:8/17/2011
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Description: A 90 grams of tofu contains only 50 kcal, eat less meat and tofu, maintain protein intake at the same time, good for weight loss. In addition, the tofu is considered ideal for yoga practice with food.