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WAVELENGTH RANGES FOR CHROMA'S MULTIBAND FILTER SETS

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WAVELENGTH RANGES FOR CHROMA'S MULTIBAND FILTER SETS Powered By Docstoc
					WAVELENGTH RANGES FOR CHROMA’S MULTIBAND FILTER SETS
This is a general reference for the nominal bandpass ranges of our multiband sets. In-
house tolerances vary from set to set.

51000 DAPI/FITC

Violet Excitation Band for DAPI:     401 - 418nm
Blue Excitation Band for FITC:       480 - 500nm
-------------------------
Blue Emission Band For DAPI:         450 - 465nm
Green Emission Band for FITC:        512 - 560nm


51001 DAPI/TRITC

Violet Excitation Band for DAPI:     392 - 417.5nm
Green Excitation Band for TRITC:     505 - 550nm
------------------------
Blue Emission Band for DAPI:         452 - 480nm
Red Emission Band for TRITC:         580 - 660nm

51002 DAPI/PROPIDIUM IODIDE

Violet Excitation Band for DAPI:     392 - 417.5nm
Green Excitation Band for PI:        505 - 550nm
------------------------
Blue Emission Band for DAPI:         452 - 480nm
Red Emission Band for PI:            580 - 660nm

51003 DAPI/TEXAS RED

Violet Excitation Band for DAPI:             400 - 410nm
Yellow Excitation Band for Texas Red:        552 - 580nm
------------------------
Blue Emission Band for DAPI:                 445 - 470nm
Red Emission Band for Texas Red:             610 - 670nm

51004V2 FITC/TRITC

Blue Excitation Band for FITC:       475 - 492.5nm
Green Excitation Band for TRITC:     545 - 565nm
-------------------------
Green Emission Band for FITC:        503 - 533nm
Orange Emission Band for TRITC:      582 - 622nm
51005 FITC/PROPIDIUM IODIDE

Blue Excitation Band for FITC:     490 - 505nm
Green Excitation Band for PI:      560 - 585nm
-------------------------
Green Emission Band for FITC:      515 - 545nm
Red Emission Band for PI:          600 - 652nm

51006 FITC/TEXAS RED

Blue Excitation Band for FITC:           490 - 505nm
Green Excitation Band for Texas Red:     560 - 585nm
-------------------------
Green Emission Band for FITC:            515 - 545nm
Red Emission Band for Texas Red:         600 - 652nm

51007 Cy3/Cy5

Green Excitation Band for Cy3:     532 - 555nm
Red Excitation Band for Cy5:       620 -645nm
--------------------------
Orange Emission Band for Cy3:      570 - 600nm
NIR Emission Band for Cy5:         662 - 725nm

51008 FITC/Cy5

Blue Excitation Band for FITC:     467 - 487nm
Red Excitation Band for Cy5:       600 - 645nm
---------------------------
Green Emission Band for FITC:      500 - 570nm
NIR Emission Band for Cy5:         670 - 825nm

51009 FITC/Cy3

Blue Excitation Band for FITC:     475 - 495nm
Green Excitation Band for Cy3:     555 -575nm
---------------------------
Green Emission Band for FITC:      510 - 535nm
Red Emission Band for Cy3:         595 - 670nm
51010 DAPI/CHLOROPHYLL

Violet Excitation Band for DAPI:        400 - 415nm
Blue Excitation Band for Chlorophyll:   475 - 500nm
----------------------------
Blue Emission Band for DAPI:            435 - 455nm
Red Emission Band for Chlorophyll:      595 - 650nm

51011 DAPI/Cy3

Violet Excitation Band for DAPI:        405 - 420nm
Green Excitation Band for Cy3:          537 - 555nm
----------------------------
Blue Emission Band for DAPI:            467 - 490nm
Orange Emission Band for Cy3:           575 - 620nm

51012 DAPI/GFP

Violet Excitation for DAPI:             402 - 430nm
Blue Excitation for GFP:                490 - 515nm
----------------------------
Blue Emission Band for DAPI:            455 - 472nm
Green Emission Band for GFP:            535 - 570nm

51013 DAPI/Cy5 - SINGLEBAND EXCITERS

Violet Excitation Band for DAPI:        397 - 412nm
Red Excitation Band for Cy5:            622 - 645nm
----------------------------
Blue Emission Band for DAPI:            450 - 472nm
NIR Emission Band for Cy5:              665 - 735nm

51014 BFP/GFP

Violet Emission Band for BFP:           380 - 395nm
Blue Excitation Band for GFP:           477 - 497nm
---------------------------
Blue Emission Band For BFP:             450 - 465nm
Green Emission Band for GFP:            512 - 560nm
51015 FITC/DiI

Blue Excitation Band for FITC:     475 - 490nm
Green Excitation Band for DiI:     540 - 550nm
---------------------------
Green Emission Band for FITC:      505 - 530nm
Orange Emission Band for DiI:      565 - 645nm

51016 LUCIFER YELLOW/TRITC OR DiI

Violet Excitation for Lucifer Yellow:     430 - 440nm
Green Excitation for TRITC:               535 - 555nm
-------------------------
Green Emission Band for Lucifer Yellow:   502 - 530nm
Orange Emission Band for TRITC:           570 - 660nm

51017 CFP/YFP
Violet Excitation Band for CFP:    430 - 440nm
Blue Excitation Band for YFP:      495 - 510nm
--------------------------
Blue Emission Band for CFP:        450 - 480nm
Green Emission Band for YFP:       520 - 565nm


61000V2 DAPI/FITC/TRITC

Violet Excitation Band for DAPI:   385 - 400nm
Blue Excitation Band for FITC:     475 - 492.5nm
Green Excitation Band for TRITC:   545 - 565nm
------------------------------
Blue Emission for DAPI:            450 - 465nm
Green Emission Band for FITC:      503 - 533nm
Orange Emission Band for TRITC:    582 - 622nm

61001 DAPI/FITC/PROPIDIUM IODIDE

Violet Excitation Band for DAPI:   395 - 410nm
Blue Excitation Band for FITC:     490 - 505nm
Orange Excitation Band for PI:     560 - 585nm
------------------------------
Blue Emission Band for DAPI:       450 - 475nm
Green Emission Band for FITC:      515 - 545nm
Red Emission Band for PI:          600 - 652nm
61002 DAPI/FITC/TEXAS RED

Violet Excitation Band for DAPI:         395 - 410nm
Blue Excitation Band for FITC:           490 - 505nm
Orange Excitation Band for Texas Red:    560 - 585nm
------------------------------
Blue Emission Band for DAPI:             450 - 475nm
Green Emission Band for FITC:            515 - 545nm
Red Emission Band for Texas Red:         600 - 652nm


61003 DAPI/FITC/Cy5

Violet Band for DAPI:              395 - 408nm
Blue Excitation Band for FITC:     465 - 485nm
Red Excitation Band for Cy5:       575 - 620nm
-----------------------------
Blue Emission Band for DAPI:       430 - 450nm
Green Emission Band for FITC:      515 - 550nm
NIR Emission Band for Cy5:         650 - 730nm

61004 DAPI/Cy3/Cy5

Violet Excitation Band for DAPI:   400 - 415nm
Green Excitation Band for Cy3:     540 - 555nm
Red Excitation Band for Cy5:       617 - 647nm
-----------------------------
Blue Emission Band for DAPI:       450 - 470nm
Orange Emission Band for Cy3:      565 - 600nm
NIR Emission Band for Cy5:         660 - 720nm

61005 FITC/Cy3/Cy5

Blue Excitation Band for FITC:     477 - 495nm
Green Excitation Band for Cy3:     545 - 570nm
Red Excitation Band for Cy5:       635 - 660nm
-----------------------------
Green Emission Band for FITC:      505 - 530nm
Orange Emission Band for Cy3:      580 - 615nm
NIR Emission Band for Cy5:         675 - 720nm
61006 FITC/TEXAS RED/Cy5

Blue Excitation Band for FITC:            470 - 495nm
Yellow Excitation Band for Texas Red:     570 - 585nm
Red Excitation Band for Cy5:              645 - 665nm
------------------------------
Green Emission Band for FITC:             515 - 555nm
Red Emission Band for Texas Red:          595 - 630nm
NIR Emission Band for Cy5:                675 - 725nm


84000 DAPI/FITC/Cy3(TRITC)/Cy5 - SINGLE EXCITERS

UV Excitation Band for DAPI:              340 - 380nm
Violet Excitation Band for DAPI:          400 - 410nm
Blue Excitation Band for FITC:            480 - 500nm
Green Excitation Band for Cy3 or TRITC:   540 - 565nm
Red Excitation for Cy5:                   622 - 645nm
-----------------------------
Blue Emission Band for DAPI:              455 - 470nm
Green Emission Band for FITC:             515 - 530nm
Red Emission Band for Cy3 or TRITC:       580 - 610nm
NIR Emission Band for Cy5:                655 - 715nm

86000 SINGLEBAND EXCITERS & EMITTERS

UV Excitation Band for DAPI:              340 - 380nm
Violet Excitation Band for DAPI:          400 - 410nm
Blue Excitation Band for FITC:            480 - 500nm
Green Excitation Band for Cy3 or TRITC:   540 - 565nm
Red Excitation for Cy5:                   622 - 645nm
-----------------------------
Blue Emission Band for DAPI:              430 - 480nm
Green Emission Band for FITC:             510 - 545nm
Red Emission Band for Cy3 or TRITC:       580 - 655nm
NIR Emission Band for Cy5:                665 - 700nm
86001 JP3 for EGFP & EYGFP

Blue Excitation Band for EGFP:    450 - 470nm
Green Excitation Band EYFP:       513 - 533nm
-----------------------------
Green Emission Band for EGFP:     490 - 510nm
Yellow Emission Band for EYGFP:   543 - 593nm

86002 CFP/YFP

Violet Excitation Band for CFP:   431 - 441nm
Blue Excitation Band for YFP:     493 - 507nm
-----------------------------
Blue Emission Band for CFP:       455 - 485nm
Green Emission Band for YFP:      520 - 550nm

86003 BFP/EGFP

UV Excitation Band for BFP:       365 - 395nm
Blue Excitation Band for EGFP:    475 - 500nm
-----------------------------
Blue Emission Band for BFP:       425 - 465nm
Green Emission Band for EGFP      510 - 560nm

86005 BFP/YFP

UV Excitation Band for BFP:       365 - 395nm
Green Excitation Band for YFP:    490 - 510nm
----------------------------
Blue Emission Band for BFP:       445 - 485nm
Green Emission Band for YFP:      520 - 550nm

88000 QUAD SET - SINGLE EXCITERS

UV Excitation for DAPI:                 340 - 380nm
Blue Excitation Band for FITC:          495 - 505nm
Green Excitation Band for TEXAS RED:    560 - 575nm
Red Excitation Band for Cy5:            645 - 660nm
------------------------
Blue Emission Band for DAPI:            465 - 485nm
Green Emission Band for FITC:           520 - 545nm
Red Emission Band for TEXAS RED:        590 - 630nm
NIR Emission Band for Cy5:              675 - 735nm

				
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posted:8/15/2011
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