Ryan Williams – 2/17/10 – Micropropagation II Page 1
T11 – Micropropagation of Potato by Node Culture and Microtuber Production
By Michael E. Kane
Potatoes are a very important food crop worldwide, but they have a large number of
pathogens that infect them. They are especially susceptible to having pathogens spread quickly
through populations because they are vegetatively propagated through tubers. Because of this,
potato growers frequently need to start with disease eradicated seed potatoes which are produced
One way to do this is to use node culture with individual nodes laid horizontally on some
medium. These nodes produce shoots which can be subcultured or acclimated to grow in the
The second way to do this is by producing microtubers in vitro. These microtubers can
then be planted just like normal seed potatoes and appear to grow just as well as normal potatoes.
The rest of this chapter is a lab protocol for doing node and microtuber micropropagation
from pre-established sterile cultures.
Microtubers sound really cool. I would love to make some of these and grow them up into full
T12 – Micropropagation of Lilacs
By Deborah D. McCown and Andrew J. Daun
Lilacs are generally a easy type of plant to establish in culture. They also reproduce very
quickly. This chapter contains protocols for an experiment involving primary culture of lilac
shoots and then subsequent experimentation on how their growth is affected by PGRs.
T13 – Micropropagation and In Vitro Flowering of Rose
By Michael E. Kane
Roses were one of the first ornamental plants to be domesticated and they are often
propagated through asexual techniques. Micropropagation hasn’t been too successful with roses
commercially, but this is a subject of current research. This chapter contains protocols to
establish cultures using nodal explants, propagate the plants by Axillary shoot proliferation, and
then root the plants ex vitro. This lab also should allow us to experience in vitro flowering of the
roses if it is done correctly.
This is so cool that the roses will make miniature flowers in vitro.
1. Why do farmers have to plant seed potatoes instead of just planting part of last year’s harvest?
2. What are two methods that can be used to micropropagate potatoes in culture?
3. True or False, M&S media is the best recipe to use for woody plants such as lilacs.
4. True or False, No plants will bloom in vitro because it is too stressful of a growing situation.
5. True or False, Establishing our own primary cultures instead of buying premade cultures adds
significantly to the time of an experiment.