Red/ET Recombination Kits
A powerful tool for modifying any DNA vector, be it a high copy plasmid, cosmid, BAC, YAC, etc. Allows modifications independant of size or restriction sites Application Modification of E.coli chromosome Modification of E.coli chromosome without leaving a selection marker Subcloning from E.coli chromosome Subcloning from BAC, PAC, Cosmid Subcloning from high copy plasmid BAC shaving (tailoring of BAC insert) BAC stitching (fusion of two BAC inserts) Deletion of unwanted sequences BAC, PAC, Cosmid Deletion of unwanted sequences high copy plasmid Deletion without leaving a selection marker BAC, PAC, Cosmid Deletion without leaving a selection marker high copy plasmid Insertion of sequences accompanied by a selection marker Insertion of sequences without leaving a selection marker Fusion constructs Fusion constructs without leaving a selection marker Generation of a reporter construct (reporter not included) Generation of a knock out construct (eukaryotic selection marker not included) Conditional targeting construct (eukaryotic selection marker not included) Transgenic construct (reporter not included); e.g. starting with a BAC Transgenic construct without leaving a selection marker Viral engineering Plant DNA engineering in E.coli Plant DNA engineering in E.coli without leaving a selection marker
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Quick & Easy Kit +++
Counterselection Kit ++ +++
Subcloning Kit
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++ secondary application + tertiary application Gene Bridges GmbH, Tatzberg 47-51, 01307 Dresden, Germany Telephone: +49 351 796 5657 Fax: +49 351 796 5655 e-mail: info@GeneBridges.com www.genebridges.com