Sample disposal and waste management

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					Central Veterinary Laboratory, S.O.P.

Standard Operating Procedure for Waste Management and Disposal Technique
1. Scope The present SOP manual on Waste Management and Disposal technique outlines the disposal and waste management of various tissue or carcass submitted for the diagnosis of diseases/conditions and materials used on these processes. It recommends routine waste collection procedure as well as the special procedure for transport of the waste materials, organisms from each unit of the lab and their transportation in hygienic way. This document would work as national reference method for Waste Management and Disposal technique in the laboratory using standard procedure. The various methods and the specific procedures for a method outlined herein this manual shall be applied to the sample disposal and waste management to the central veterinary laboratory. 2. Introduction Waste is anything that is to be discarded. In laboratories, decontamination of wastes and their ultimate disposal are closely interrelated. In terms of daily use, few if any contaminated materials will require actual removal from the laboratory or destruction. Most glassware, instruments and laboratory clothing will be reused or recycled. The overriding principle is that all infectious materials should be decontaminated, autoclaved or incinerated within the laboratory. The principal questions to be asked before discharge of any objects or materials from laboratories that deal with potentially infectious microorganisms or animal tissues are: 1. Have the objects or materials been effectively decontaminated or disinfected by an approved procedure? 2. If not, have they been packaged in an approved manner for immediate on-site incineration or transfer to another facility with incineration capacity? 3. Does the disposal of the decontaminated objects or materials involve any additional potential hazards, biological or otherwise, to those who carry out the immediate disposal procedures or who might come into contact with discarded items outside the facility? 1 Prepared by: Dr. Banshi Sharma Senior Veterinary Officer CVL

Central Veterinary Laboratory, S.O.P. Different samples in the lab shall be collected to diagnose diseases by using standard procedure. The help desk is equipped to handle waste materials and used and unused samples and submit it to washing unit of the lab. Solid waste shall put in biological pit as specified in SOP of collection, transportation, sample submission and reporting, annex(B). 3. Code of Conduct 3.1.1. Purpose    To maintain a hygienic working environment in the laboratory room; and To secure general health status of the personnel working in with the laboratory. To safely disposal of samples and materials used or unused in the lab.

3.1.2. Requirements Hand glove, boot and mask shall wear at the time of disposal. 3.1.3. Procedure Please wear PPE as mention in the protocol of respective laboratory unit, special attention shall be taken for handling used samples of suspected HPAI. 3.2. Occupational Health and Safety HPAI sample should be taken with due care. Zoonotic disease suspected samples must handle with care. Wear globes and mask as per requirement. 3.2.1. Purpose   To ensure occupational health by carefully exploring and adopting personal safety measures at various steps of procedures involved in the SOP of collection, transportation, sample submission and reporting. To safely dispose used and unused materials in the laboratory.

4.0 General Procedure

Steam autoclaving is the preferred method for all decontamination processes. Materials for decontamination and disposal should be placed in containers, e.g. autoclavable plastic bags that are colour-coded according to whether the contents are to be autoclaved and/or incinerated. Autoclave has been done at 121 0C, 15 pound pressure for 30 minutes. Alternative methods may be envisaged only if they remove and/or kill microorganisms. 2 Prepared by: Dr. Banshi Sharma Senior Veterinary Officer CVL

Central Veterinary Laboratory, S.O.P.
Handling and disposal procedures for contaminated materials and wastes

Identification and separation system for infectious materials and their containers should be adopted. National and international regulations must be followed. Categories should include: 1. Non-contaminated (non-infectious) waste that can be reused or recycled or disposed of as general, “household” waste. 2. Contaminated (infectious) “sharps” – hypodermic needles, scalpels, knives and broken glass; these should always be collected in puncture-proof containers fitted with covers and treated as infectious. 3. Contaminated material for decontamination by autoclaving and thereafter washing and reuse or recycling. 4. Contaminated material for autoclaving and disposal. 5. Contaminated material for direct incineration. Sharps After use, hypodermic needles should not be recapped, clipped or removed from disposable syringes. The complete assembly should be placed in a sharps disposal container. Disposable syringes, used alone or with needles, should be placed in sharps disposal containers and incinerated, with prior autoclaving if required. Sharps disposal containers must be puncture-proof/-resistant and must not be filled to capacity. When they are three-quarters full they should be placed in “infectious waste” containers and incinerated, with prior autoclaving if laboratory practice requires it. Sharps disposal containers must not be discarded in landfills. Contaminated (potentially infectious) materials for autoclaving and reuse No pre-cleaning should be attempted of any contaminated (potentially infectious) materials to be autoclaved and reused. Any necessary cleaning or repair must be done only after autoclaving or disinfection. Contaminated (potentially infectious) materials for disposal Apart from sharps, which are dealt with above, all contaminated (potentially infectious) materials should be autoclaved in leak-proof containers, e.g. autoclavable, colour-coded plastic bags, before disposal. After autoclaving, the material may be placed in transfer containers for transport to the incinerator. If possible, materials deriving from healthcare activities should not be discarded in landfills even after decontamination. 3 Prepared by: Dr. Banshi Sharma Senior Veterinary Officer CVL

Central Veterinary Laboratory, S.O.P. If a broken containers and spilled infectious substances Broken containers contaminated with infectious substances and spilled infectious substances should be covered with a cloth or paper towels. Disinfectant should then be poured over these and left for the appropriate amount of time. The cloth or paper towels and the broken material can then be cleared away; glass fragments should be handled with forceps. The contaminated area should then be swabbed with disinfectant. If dustpans are used to clear away the broken material, they should be autoclaved or placed in an effective disinfectant. Cloths, paper towels and swabs used for cleaning up should be placed in a contaminated-waste container. Gloves should be worn for all these procedures. If laboratory forms or other printed or written matter are contaminated, the information should be copied onto another form and the original discarded into the contaminated-waste container. All broken tubes, glass fragments, buckets and the rotor should be placed in a noncorrosive disinfectant known to be active against the organisms concerned. Unbroken, capped tubes may be placed in disinfectant in a separate container and recovered. The centrifuge bowl should be swabbed with the same disinfectant, at the appropriate dilution, and then swabbed again, washed with water and dried. All materials used in the clean-up should be treated as infectious waste.
Breakage of tubes inside sealable buckets (safety cups)

All sealed centrifuge buckets should be loaded and unloaded in a biological safety cabinet. If breakage is suspected within the safety cup, the safety cap should be loosened and the bucket autoclaved. Alternatively, the safety cup may be chemically disinfected.

5. Transferring the waste materials The sources of waste materials in the lab are different units of the lab. These waste are put separately for organisms contaminated, biological hazard materials, hypodermic needles, carcasses and glass wares etc. These materials must be autoclaved except postmortem carcasses. The carcass shall be put in biological pit from the bucket of the postmortem unit of the CVL. Transferring of the waste must be done in morning or afternoon hour after completion of lab work.

5.1. Recording the Waste disposal 4 Prepared by: Dr. Banshi Sharma Senior Veterinary Officer CVL

Central Veterinary Laboratory, S.O.P.    Any kind of waste brought to washing unit of CVL shall be registered in the main register of washing unit. The registration of the waste disposal register shall specify date expire reagents and chemicals in detail The waste disposal kit shall be distributed to the relevant laboratory unit of CVL.

5.2. Physical Examination of the Waste materials Waste materials shall be examined against the following criteria:   Condition of the waste materials; any departure of the colour and odour from the normal condition shall be conveyed to the respective unit of CVL. Information of the waste materials.

6. Waste disposal results  The waste disposal result shall be duly signed by technician and in-charge of washing unit and postmortem unit of the lab and result interpreted by technical manager of the laboratory.

7. Record keeping A back up (official copy) of the waste result reports prepared and handed over to the chief, CVL. 8. Ensuring the safety standard and quality control of waste disposal  It would be rather a duty of respective lab to ensure the fact that the waste disposal shall be done in proper way following international norms and standards.  The respective lab shall maintain the bio-safety measure to deal with waste matters. Quality manager shall responsible for proper disposal of waste materials.

9. Feedback  The respective lab shall collect waste material in its routine work schedule and shall give feedback to washing unit of the lab and comments if any to chief, CVL. It would be considered as an endeavor in the way of quality improvement of the each and every units of CVL. 5 Prepared by: Dr. Banshi Sharma Senior Veterinary Officer CVL

Central Veterinary Laboratory, S.O.P. References: 1. The National Plant Diagnostic Network Standard Operating Procedure for: APHIS-PPQ Pest of Concern Scenario – General SOP, rev June 17, 2005 2. 3. Guidance for preparing standard operating procedures (SOPs)- United States Environmental Protection Agency EPA/600/B-07/001, April, 2007. 4. Guidelines for Establishing Quality Systems in Veterinary Diagnostic Testing Laboratories, Vienna International Centre, 4-8 September 2000

6 Prepared by: Dr. Banshi Sharma Senior Veterinary Officer CVL

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banshi sharma banshi sharma Chief Molecular biologist