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White Blood Cell Differential Lab


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1) Did you notice any abnormally high or low WBC’s? If yes, which ones?

2) A patient comes in complaining of weight loss and fatigue. The doctor draws blood and asks the
   lab to perform a WBC differential. The results come back are below. What diagnosis would you
   make based on the results?

   Neutrophils: 55%
   Eosinophils: 15%
   Basophils: 2%
   Monocytes: 6%
   Lymphocytes: 25%

3) In patients with AIDS, lymphocytes are attacked and destroyed. Why types of symptoms would you
   expect to see in a person with advanced stages of AIDS?

4) A college student is admitted to the hospital with suspected bacterial meningitis (inflammation &
   infection of the meninges). The doctor performs a spinal tap to determine whether or not her
   diagnosis was accurate. What types of leukocytes would she expect to find in the CSF if she is
   correct? Why? (No cells should be present in normal CSF).

5) Define the following:


 White Blood Cell Differential Lab                                                           Period:________

 In hospital laboratories, the white blood cell differential (diff) is a very common procedure used to
 identify illnesses. In a diff, 100 WBC’s are counted and identified based on their type. Knowing the
 normal ranges for these cells allows you to identify abnormalities and help diagnose the patient.

 Part 1: Identifying Leukocytes (& other cells)

 Obtain a blood smear slide. Focus your slide under low (10x) power, and then move to high (400x)
 power. Find and identify the cells listed below. Then have your teacher initial that you have correctly
 identified the cell. Draw, in color, what you see in the circles. On at least one of your drawings,
 identify the erythrocytes & platelets. You should draw the RBC’s & platelets on all.

LYMPHOCYTE                      MONOCYTE                          EOSINOPHIL                  NEUTROPHIL


                                   Part 2: Performing a WBC Differential

                                   In order to perform a correct differential, you must count 100 different
                                   WBC’s. Split the work between you and your partner, each counting 50.
                                   As one partner counts and identifies cells, the other should be making
                                   tally marks. Answer the questions on the back individually – each person
                                   should hand in a lab sheet.

                                   NEUTROPHIL     LYMPHOCYTE     MONOCYTE       EOSINOPHIL     BASOPHIL

                                   YOUR PERCENTAGES:

                                   ________       ________       ________       ________       _______

                                   NORMAL PERCENTAGES:

                                   ________       ________       ________       ________       _______

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