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					Shrimp Surveillance &
Certification Program
                  Allen C. Riggs DVM, MS
                  Veterinary Medical Officer

Aquaculture Development Program – Disease Prevention (ADP-DP)

              Hawaii Dept of Agriculture (HDOA)
Voluntary partnership between Hawaii
marine shrimp broodstock and seedstock
producers with the Hawaii Department of

3rd party oversight with chain of custody
sample collection and handling to USDA-
APHIS certified crustacean diagnostic lab
   SSCP Protocol Overview
The primary goal is for marine broodstock and
seedstock producers to acquire Specific
Pathogen Free (SPF) status for their facility

The Program is based on polymerase chain
reaction (PCR) testing protocols following OIE
guidelines for the 7 currently listed OIE marine
shrimp pathogens

24 months continuous negative testing is
required for SPF status
              Origin of SSCP
Initial surveillance protocols were started in the late
1990’s for each operations internal biosecurity

This was not the standardized 3rd party HDOA program
of today but was a good starting point for several

Most important is this was a voluntary program that the
industry considered important and the cost/time involved
was worth the investment

This mindset facilitated the more structured current
SSCP program
International Import Regulations
 Only recently have most international
 countries required health certification and
 specific pathogen testing documentation
 for live marine shrimp broodstock and
 seed stock to enter their countries

 No uniformity but the current OIE
 crustacean disease list is the best
 template for a basic SSCP program
Marine shrimp life/farming cycle
 Adult female       day 0         stenohaline
 Fertilized egg     day 0-5
 Nauplius larvae    day 1-2
 Zoea larvae        day 2-7
 Mysis larvae       day 7-10

 PL (post-larvae)   day 10-25     euryhaline
 Juvenile           day 25-90
 Subadult           day 90-150

 *Lightner – Shrimp Disease and Diagnosis - 2006
Hawaii Marine Broodstock Shrimp
12 marine broodstock shrimp producers currently in
operation on four islands in Hawaii ( Oahu, Molokai,
Kauai and Big Island )

Most important component of shrimp farming in Hawaii –
3+ Million dollar annual farmgate value

Typical marine broodstock shrimp is 11 -12 months old
with a reproductive life span of 3 to 6 months maximum

For intra-island, interstate( to mainland US), or
international export must have health certification and
specific pathogen negative status documentation
   Hawaii Seedstock Shrimp
Again these 12 operations can also supply
seedstock to other broodstock producers or to
off-site commodity food shrimp producers

Typically PL after day 8 (osmoregulate) to day
25 are sold for growout stocking

For intra-island transfer to second party grow-
out operations, interstate( to mainland US), and
international exports health certification and
specific pathogen negative testing
documentation is also required
   SSCP Sampling Protocols
New Facility
Export Facility
SPF Facility

Tissues sampled: hemolymph, pleopods, and fecal
strands for survival methods with broodstock
Whole or partial PL for lethal methods with smaller
Only one shrimp farm/site is visited per 24 hours to allow
for adequate disinfection of staff/equipment
SSCP- SPF shrimp definition
The SSCP definition for SPF shrimp refers to a stock that is subject
to a regular PCR surveillance program for the 7 current OIE listed
marine shrimp pathogens with a minimum of 24 months continuous

USDA-APHIS certified lab to diagnose crustacean diseases must be

OIE guidelines for surveillance protocols to be followed

No farm submitted surveillance samples …only ADP/HDOA 3rd party
chain of custody sample collection used in the SSCP

No requirements for biosecurity or husbandry type in this definition
                          New Facility
   Site specific
   Test schedule will be months 1, 3, 6, 12, 18, and 24 months
   A total number of 60 shrimp will be tested for all 7 listed diseases – 95%
   confidence/ 5% prevalence for populations up to and over 100,000 shrimp
   ( Section 3.2 Diagnosis in subclinical crustacean carriers: Manual of Dx
   Tests for AA - 2006)
   Sampling regime includes all life stages on site
   Shrimp used to stock this facility must originate from a SPF facility

Virus Group        Vial      System       Species      Life Stage    Tissue

BP/MBV             A1-12

TSV/YHV/IMNV       B1-12

IHHNV/WSSV         C1-12
              Export Status Facility
Site specific
Successful completion of three negative sample sets in the New farm
   program – months 1, 3, and 6
Facility now allowed to ship/export live shrimp with health documents
SPF language not used to describe stocks
Testing will continue at months 12, 18, and 24 with the 60
   shrimp/sample set to acquire SPF certification

Virus Group      Vial      System     Species     Life Stage   Tissue

BP/MBV           A1-12

TSV/YHV/IMNV     B1-12

IHHNV/WSSV       C1-12
Specific Pathogen-Free Facility
Site specific
Shrimp used to stock this facility site must originate from a SPF facility
Testing schedule will continue every 6 months
Total number of shrimp tested can be reduced to 30 animals
(Section 3.3 Testing for verification or maintenance of freedom from specific
diseases: Manual of Dx Tests for AA – 2006)
Sampling regime includes all life stages on site
SPF language added to health certification reports

Virus Group     Vial   System Species          Life Stage    Tissue

BP/MBV          A1-6
TSV/YHV &       B1-6
Industry/State Partnership: Industry
 True partners share both costs and benefits in a
 business relationship

 The shrimp producer in the SSCP is responsible
 for all the PCR testing fees:

 SPF operations testing 30 shrimp for each of the
 7 currently listed OIE diseases is
 $972.00/sample set every 6 months or
 $1944.00/year ( current University of Arizona –
 Aquatic Pathology Laboratory prices)
Industry/State Partnership: Industry
 For New Farms or Export Status Farms working toward
 SPF Certification, the 60 animal sample set for each of
 the 7 currently listed OIE diseases must be followed

 The cost per sample set is $1944.00

 Year 1 with 4 sample sets (months 1, 3, 6, and 12) is

 Year 2 with 2 sample sets ( months 18 and 24) is
  Industry/State Partnership: State
ADP-DP is responsible for all costs involved in ADP-DP
  staff traveling to each SSCP farm site for chain of
  custody documentation, both on Oahu and the outer
  islands: airfare, rental car or state vehicle costs

ADP-DP staff time is covered by HDOA budget

ADP-DP provides all necessary vials, fixatives and
  collection instruments and covers shipping costs to UAZ-

The ADP-DP/HDOA component of the SSCP is a 100%
  Hawaii State funded program
   Future challenges/questions
When to add emerging diseases to SSCP
   NHP – Necrotizing hepatopancreatitis
This disease is under study by OIE and
likely to be listed in the future

When to remove delisted diseases from the
 SSCP (BP and MBV). Many countries still
 require surveillance data on these two
 diseases for live shrimp imports

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