DNA Extraction

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					          DNA Extraction




http://www.vivo.colostate.edu/hbooks/genetics/biotech/manip/conc.html
                                      Definition
 DNA Extraction is
  the isolation and
  purification of DNA
  (deoxyribonucleic
  acid)



  http://academy.d20.co.edu/kadets/
  lundberg/images/dna.gif
                    Examples
 DNA extraction is used
  to isolate…
    Mitochondrial DNA
    Genomic DNA
                             http://www.davidkfaux.org/shetlandislandsmtDNA.html

 DNA can be extracted
  from…
    Cells or tissues
    Environmental samples

                                  http://faculty.uca.edu/~benw/biol1400/pictures/
               Non-examples
 DNA Extraction is
  not used to…
   Isolate proteins or
    RNA
   Give information
    about gene
    expression


                          http://iwrwww1.fzk.de/biostruct/
     Downstream Applications
 After DNA is extracted, it is used as a
  template in further molecular techniques such
  as…

   PCR (polymerase chain reaction)
   RFLP (restriction fragment length polymorphism)
   Southern Blotting
                     DNA Analysis
 Downstream techniques
  can:


   Reveal how organisms are
    related
                                http://www.biocompare.com/itemdetails.asp?itemid=687320


   Identify cryptic species

   Locate mutations in DNA



                               http://www.cdc.gov/hemochromatosis/training/images/dna.jpg
               Quick Quiz
What is the purpose of DNA extraction?

  a. To learn how to safely work with chemicals

  b. To quantify the amount of DNA per cell

  c. To determine how organisms are related

  d. To purify DNA from cells
Qiagen DNA Extraction
     Tissue Lysis. Detergents lyse membranes.
         Proteinase K breaks down proteins.




  Sample loading onto column. Nucleic acids bind
  to membrane and other components flow through.




   Column washing. Removes any contaminants.




   Elution of DNA. DNA is released from column
   and ready for use in downstream applications.
                Quick Quiz
The purpose of the column is to:

   a. Separate DNA from RNA

   b. Separate DNA molecules from cellular debris

   c. Remove bacterial contaminants

   d. Help suspend DNA in elution buffer
END
                 Resources

 DNA Extraction Handout

 DNEasy Tissue Kit Handbook

 http://www1.qiagen.com/literature/protocols/
  DNeasyTissue.aspx
  California State Chemistry Standards
Grade 8
  6c. Living organisms have many different kinds of molecules…

Grades 9-12
  6. Solutions are homogenous mixtures of two or more
     substances

  7. Energy is exchanged or transformed in all chemical
     reactions and physical changes of matter

  8. Chemical reaction rates depend on factors that influence the
     frequency of collision of reactant molecules
     California State Biology Standards
Grade 7
  2e. DNA is the genetic material of living organisms and is
  located in the chromosomes of each cell
  3a. Genetic variation and environmental factors are causes of
  evolution and diversity of organisms

Grades 9-12
  2. Mutation and sexual reproduction lead to genetic variation in
  a population

  7. The frequency of an allele in a gene pool of a population
  depends on many factors and may be stable or unstable over
  time
              California State
     Investigation and Experimentation
                 Standards
Grades 7, 9-12
  a/b. Select and use appropriate tools and technology to perform
  tests, collect data, analyze relationships, and display data


Grade 8
  a. Plan and conduct a scientific investigation to test a hypothesis
              National Standards

Grades 6-12

Content Standard A: Science as Inquiry

Content Standard C: Life Science


Content Standard E: Science and Technology

				
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posted:7/28/2011
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