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 =DOCUMENT DESCRIPTION   DOCUMENT CONTROL NUMBER   DATE RECEIVED
SEHQ-o-i66qi              Bnov                       a *v '

COMMENTS:                 2~Xi5




                 oors NOT CONTAIN CBI
                                                                            Andrea V.Malinowski
                                                                            Corporate Counsel


                                                                            DuPont Legal
                                                                            Wilmington Office Buildings
                                                                            1007 Market Street
                                               P~
                                             I EL3-7j:6Wilmington,                      DE 19898          Fa
                                                                            andrea.v.malinowskigusa.dupont.com E-mail

February 3, 2011

VIA CERTIFIED MAIL
Certified Mail Receipt No. 7001-2510-0006-9113-2343

Attn: TSCA Declassification Coordinator                                    Public Copy
U.S. Environmental Protection Agency                                       No CBI
Office of Pollution Prevention and Toxics
Document Control Office (7407M)
Washington, D.C. 20460

Re:        Declassification Activity-TSCA §8(e) Submission
           8EIIQ Bar Code Number: 8EHQ-07-16691
           Other EPA Identifier(s): 88070000109/S; 8EHQ-1006-16691A
           DuPont-13654 (1-028)
           Supplemental Submission - Revised Public Copy of Report

Dear TSCA Declassification Coordinator:
This submission is made in connection with the EPA 2010 CBI Declassification Challenge
initiative.

Please find enclosed a revised public copy of the above-identified report, which was previously
submitted to EPA and assigned the 8EHQ number referenced above. Any information still
claimed as confidential business information (CBIl) in the attached report has been redacted and
replaced by brackets. The originally assigned 8EHQ number (as bar coded on the submission)
has been added by the submitter to the first page of the enclosed revised public copy of the report.
Other EPA identifying numbers stamped on the submission or provided on the EPA website are
referenced above.

Very truly yours,



Andrea V. Malinowski                                             0769


Enclosure
  *DuPont-13654              -   53 pages



                                            COTAINS NO CBI




  ElI du Pont de Nernouro and Company
                                    Revised Public Copy
                                    Originally Assigned 8EHQ Number: 8EHQ-07-16691
                                    Company Sanitized - No C13I
                                                                           DuPont-13654



                                TRADE SECRET


                                    Study Title
 H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats Including
                Histopathology Examination of the Respiratory Tract




                 AUTHOR: Michael P. DeLorme, Ph.D.

    STUDY COMPLETED ON: December 14, 2004

PERFORMING LABORATORY: E.L. du Pont de Nemours and Company
                       Haskelsm Laboratory for Health and Environmental Sciences
                       Elkton Road, P.O. Box 50
                       Newark, Delaware 19714-0050

 LABORATORY PROJECT ID: DuPont-13654

 WORK REQUEST NUMBER:       14799

   SERVICE CODE NUMBER: 700




                                    Page 1 of 53
H-26027-. Inhalation Approximate Lethal Concentration (ALCI in Male Rats
Including Histopathology Examination of the Respiratory Tract                                  DuPont-1365 4



                                            CERTIFICATION

We, the undersigned, declare that this report provides an accurate evaluation of data obtained
from this study.




             Evaluatin by:                                    .~                    134ZI4bv -z'A
                              9Lev= R-Frame- D.V34. Ph.D., Diploma-c A.C.VJP              Date
                              Principal Research Pathologist and Research Manager




   Enued by Study Director.                                                               Zt        r
                                            Research Toxicologist
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                                                        DuPont. 13654



                                          TABLE OF CONTENTS

                                                                                                                                     Page

CERTIFICATION ...................... ..................................
                                                   ...................                                                                      2
STUDY INFORMATION                 .   ...............................                        ................................               4
SUMMARY...................................................................................... 5

INTRODUCTION .........................                                   ...................         .............................          7
STUDY DESIGN ......................................................................................                                         7
MATERIALS AND MIETHODS ....................                  ......................................       .......... 7
  A. Test Substance ...................................................................................              7
  13. Test Species .....................................................................................             7
  C. AnimaliHusbandry ............. ................ ............................. 8              ..................
  D. Inhalation Exposure System ....................................................................                 9
  E. Characterization of Chamber Atmosphere .............. ......................................                    9
  F. Anatomic Pathology       Evaluation.................................... .......................... 10
RESULTS AND DISCUSSION......................................................................             11
  A. Exposure Conditions...........................................................................I1
  B. Mortality. Clinical Signs, and Body Weights ..............................                ......... 11........
    C. Anatomic Pathology Evaluation ........ .................................................                                           1I1
CONCLUSIONS ........................................................................... 12
RJECORDS AND SAMPLE STORAGE............................................................                                                   12
REFERENCES ...................................................................................                                       .    12
TABLES.......................................................................................... 13
TABLE I CHARACTERIZATION OF TEST ATMOSPHERES AND ASSOCIATED ANIMAL MORTALITY. .14
TABLE 2 INCIDENCE OF GROSS OBSERVATIONS IN MALE RATS --- -- ----------------------                                                          I
                                                                                                                                         .. 15
TABLE 3 INCIDENCES AND LESION GRADES OF MICROSCOPIC OBSERVATIONS IN MALE RATS -- 17
APPENDICES ...........................................................................................                                    22
APPENDIX A DETERMINATION OF LC50                     .....................    .................                                           23
APPENDIX B INDIVIDUAL ANIMAL PATHOLOGY DATA                                   .
                                                                             ...    ... ...................           .. ... ... 26




                                                             -3    -
H-26027- Inhalation Approximate Lethal Concentration (ALCI in Male Rats
Including Histopathology Examination of the Respiratory Tract                            DuPont-1I3654




                                      STUDY INFORMATION

                  Substance Tested: iZonylo 9529

                  Synnm/Codes: -           H-26027
                                          0Plant Lot #1

                   Haskell Number: 26027

                       Composition:      -         Partially Fluorinated Urethane Polymer
                                                   Partially Fluorinated Urethane Copolymer
                                                    Acrylic Copolymer
                                                    Water     adCtoi         iprat
                                                        HeyeeGlycol


                 Known Impurities: Not supplied by the sponsor

           Physical Characteristics: White liquid

                            Stabilitv. The test substance appeared to be stable under the
                                       conditions of the study-, no evidence of instability was
                                       observed.

                            Sponsor: E.I. du Pont de Nemours and Company
                                     Wilmington, Delaware 19898
                                     U.S.A.

        Stu~dy Initiated/Comnpleted: September 9, 2003 / (see report cover page)

       In-Life Initiated/Completed: September 23, 21003 /October 22, 2003




                                                   -4-
H-26027. Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                         DuPont- 13654




                                              SUMMARY
The purpose of this study was to determine the 4-hour inhalation approximate lethal
concentration (ALC) of 11-26027 in male rats as well as determine the no-observable-effect level
for histopathology changes in the respiratory tract. Five groups of 6 male and 2 groups of
10 male Crl:CD4 (SD)IGS BR rats each were exposed to atmospheres of H-26027 in air. Test
atmospheres were generated by aerosolization. Since H-26027 is an aqueous dispersion of
fluorinated and acrylic polymers, the total airborne (wet aerosol) concentration was measured
gravimetrically and H-26027 solids component of the test atmospheres measured using a
desiccation and gravimetric technique. One group of 4 male rats was exposed to air only. Rats
were exposed nose-only for a single. 4-hour period. Following exposure, surviving rats were
retained for a 1-, 13-, or 14-day recovery period. Rats were weighed and observed for clinical
signs of toxicity during the recovery period. Rats were exposed to mean wet aerosol
concentrations of 1.0, 10, 110, 120, 510, 1000, or 5900 mg/rn 3 H-26027 (0.95, 10, 94, 110,410,
560, or 940 mg/rn3 H-26027 solids, repsectively). The mass median aerodynamic diameters for
the aerosol atmosphere inhaled by the animals ranged from 0.67 to 5.1 Jim.

Fifty percent mortality was observed following exposure to 5900 mg/rn 3 (940 mg/rn3 H-2 6027
solids) and exposure to 510 and 1000 mg/ni 3 H-26027 W41 and 560 mg/rn3 H-26027 solids.
respectively) resulted in an 83% incidence of death. No mortality was observed at the next
lowest concentration of 110 - 120 mg/rn 3 (94 - 110 mg/n 3 11-26027 solids, respectively). Deaths
generally occurred within 1 day of exposure. Some notable clinical signs of toxicity observed
during this study included colored discharge from either the nose or eyes. These signs were
typically observed immediately postexposure and had resolved by 2 days postexposure. In
addition, rats generally exhibited a slight, I to 121% on average, body-weight losses within I day
of exposure but began to regain weight by 2 days postexposure.

Animals exposed to 120 mg/ni 3 total wet 11-26027 aerosol and less were subject to
histopathology examination of the respiratory tract. Exposure to 120 mg/rn 3 total H-26027
resulted in degeneration and necrosis of the ventral laryngeal mucosa with focal areas of
squamous differentiation and/or ulceration. Mild to moderate inflammation in the submucosa
and necrosis of cartilage dorsal to the laryngeal saccule were also present in the larynx 24-hours
postexposure. Animals exposed to 10 mg/rn 3 11-26027 demonstrated a slight inflammation of the
submucosa in 3 of 5 rats 24-hours postexposure. Test substance-induced changes at 14-days
postexposure were limited to residual cartilage necrosis in animals exposed to 110 mg/n 3 . The
inflammation observed at 24-hours postexposure in the rats exposed to 10 mg/rn 3 Was resolved
by 14-days postexposure. No test substance-related microscopic changes were present in the rats
exposed to 1.0 mg/rn 3 11-2602 7.

Under the conditions of this study, the approximate lethal concentration (ALC) for H-26027 is a
total wet aerosol of 5l1 mg/rn 11-26027 (410 mg/rn 3 11-26027 solids). The ALC is defined as
the lowest atmophseric: concentration tested which casuses the death of one or more of the
exposed animals. Since there concentration-repsponse curve demonstrated a steep slope between
120 and 5 10 mg/r 3 1H-26027 (051 and 83%/0 mortalitiy, respectively), an inhalation median lethal
concentration (LC 5o) was calculated since there were a suitable number of data points. In this
H-26027, Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                      DtiPont-1365 4

case, the LC more accurately represents the lethal concentration. Therefore, a LC5 o of
            50
 380 mgr 3 wet H-26027 aerosol (120 - 680 mng/rn 3 951% confidence interval) and a LC Of
                                                                                      50
300 mg/rn 3 H-26027 solids (10 -450 mg/rn3 95% confidence interval) is derived. According to
Haskell Laboratory toxicity classifications, H-26027 is considered to be moderately toxic (LCo
                                                                                             5
200 -800 mg/rn 3 ) in male rats.




                                                   -6-
H-26027. Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                          DuPont-l3654


                                          INTRODUCTION
The objective of this study was to determine a 4-hour inhalation approximate lethal
concentration (ALC) of H-26027 in male rats as well as determine a no-observable-effect level
for histopathology changes in the respiratory tract. The ALC is defined as the lowest
atmospheric concentration tested which caused the death of one or more exposed rats either on
the day of exposure or within at least 14 days following exposure. The inhalation route of
exposure was chosen based on the expected route of potential human exposure and was
requested by the sponsor.


                                           STUDY DESIGN
Five groups of 6 male rats each and 2 groups of 10 male Crl:CDf'(SD)IGS BR rats each were
exposed to atmospheres of H-26027 in air. Test atmospheres were generated by aerosolization,
and concentrations of H-26027 were measured gravimetrically. One groups of 4 male rats was
exposed to air only. Rats were exposed nose-only for a single, 4-hour period. Following
exposure, surviving rats were retained for a 1-, 13-. or 14-day recovery period.
Rats were approximately 8 weeks old and weighed between 230 and 301 grams at the time of
exposure.

Rats were observed for mortality and clinical signs of toxicity immediately after they were
removed from the restrainers following exposure. During the recovery period, all surviving rats
were observed each day for mortality. Surviving rats were weighed and observed for clinical
signs of toxicity on the day following exposure and at least twice more during the recovery
period_ At the end of the recovery period, all surviving rats were sacrificed by carbon dioxide
asphyxiation and discarded.

                                  MATERIALS AND METHODS

A.      Test Substance

The test substance, H-26027, was supplied by the sponsor as a white liquid, which is an aqueous
dispersion of fluorinated and acrylic polymers. The test substance was assumed to be stable
throughout the exposure phase of the study; no evidence of instability was observed.

B.      Test Species

Young adult, male Crl:CD'kSD)IGS BR rats were received from Charles River Laboratories,
Inc., Raleigh, North Carolina. The rats were approximately 7 weeks old on the day of arrival.

Rats have historically been used in safety evaluation studies for inhalation toxicity testing. The
Crl:CDf'kSD)IGS BR rat was selected based on consistently acceptable health status and on
extensive experience with the strain at Haskell Laboratory.



                                                   -7-
       H-26027- Inhalation Approximate Lethal COncentration (ALC) in Male Rats
       Including Histopathology Examination of the Respiratory Tract                           DuPont- 13654

*C.             Animal Husbandry

                 I.     Quarantine

       Rats were quarantined after arrival for 5 days prior to testing. During the quarantine period, rats
       were weighed and observed for clinical signs of disease 3 times.
                2.      Animal Selection

       Prior to each exposure. 6 to 12 male rats were selected for use on the study from the rats that
       were released from quarantine, had been gaining weight at a normal rate, had no overt signs of
       disease, and were the appropriate age and body weight. No attempt was made to randomly group
       animals.

                3.      Identification

       Each rat was assigned an animal number that was recorded on a card affixed to the cage. Prior to
       exposure, the tail of each animal and cage card were coded with water-insoluble pens so that
       each animal could be identified after exposure and during the recovery period.

                4.      Housing
       Except during exposure, rats were housed singly in stainless steel, wire-mesh cages suspended
       above cage boards.

 W5.                    Animal Room Environment
       Rats were housed in proximity to the inhalation chambers. Animal rooms were maintained at a
       temperature of 18-26*C (targeted to 22-24"C) and a relative humidity of 30-701%' (targeted to 40-
       60%). Animal rooms were artificially illuminated (fluorescent light) on an approximate 12-hour
       light/dark cycle. Excursions outside of these ranges were of insufficient magnitude and/or
       duration to have adversely affected the validity of the study.
                6.      Feed and Water

       Except during exposure, PMI" Nutrition International, LLC Certified Rodent LabDiete 5002 and
       tap water were available adlibitum.
                7.      Animal Health and Environmental Monitoring Program

       As specified in the Haskell Laboratory animal health and environmental monitoring program, the
       following procedures are performed periodically to ensure that contaminant levels are below
       those that would be expected to impact the scientific integrity of the study:
           .Water samples are analyzed for total bacterial counts, and the presence of coliforms, lead,
             and other contaminants.

       -    Feed samples are analyzed for total bacterial, spore, and fuingal counts.



                                                             -8 -
    H-26027. Inhalation Approximate Lethal Concentration (ALC) in Male Rats
    Inclading Histopathology Examination of the Respiratory Tract                         DaPont- 13654

      *Samples from freshly washed cages and cage racks are analyzed to ensure adequate
       sanitation by the cagewashers.

    Certified animal feed is used, guaranteed by the manufacturer to meet specified nutritional
    requirements and not to exceed stated maximum concentrations of key contaminants, including
    specified heavy metals, aflatoxin, chlorinated hydrocarbons, and organophosphates. The
    presence of these contaminants below the maximum concentration stated by the manufacturer
    would not be expected to impact the integrity of the study.

    The animal health and environmental monitoring program is administered by the attending
    laboratory animal veterinarian. Evaluation of these data did not indicate any conditions that
    affected the validity of the study.

    D.      Inhalation Exposure System

            1.      Atmosphere Generation
    Chamber atmospheres were generated by aerosolization of the test substance in air with a
    Spraying Systems nebulizer. The test substance was metered into the nebulizer with a Harvard
    Apparatus model PHI) 4400 syringe infutsion pump. Filtered, high-pressure air, metered into the
    nebulizer by a Brooks model 0OI54CFD flow controller, carried the resulting atmosphere into the
    exposure chamber. Chamber concentrations of test substance were controlled by varying the
    feed rate to the atmosphere generator.
*   Test atmospheres were exhausted through a dry-i-ce cold trap followed by an MSA
    charcoallHEPA filter cartridge prior to discharge into the fume hood.

            2.      Chamber Construction and Design

    The exposure chamber was constructed of glass (cylindrical) with a nominal internal volume of
    34 L. A polycarbonate baffle inside the chamber promoted uniform distribution of the test
    atmosphere.

            3.      Exposure Mode

    During exposure, animals were individually restrained in perforated stainless steel cylinders with
    conical nosepieces. The restrainers were inserted into a polymethylmnethacrylate faceplate that
    was attached to the exposure chamber so that the nose of each animal extended into the exposure
    chamber.

    E.      Characterization of Chamber Atmosphere

            1.      Test Substance Sampling and Analysis

    The atmospheric concentration of H-26027 was determined by gravimetric analysis at a
    minimum of 60-minute intervals during each exposure. Known volumes of chamber atmosphere
    were drawn from the sampling port through a 25 mm filter cassette containing a pre-weighed
*   glass fiber (Type AlE) filter. Immediately following the collection of the chamber atmosphere
         H-26027. Inhalation Approximate Lethal Concentration (A.LC) in Male Rats
         Including Histopathology Examination of the Respiratory Tract                          DuPont-I 3654

         sample, the filters were removed from the cassette and weighed on a Cahn Microbalance®. The
         post-sampling filter weight was recorded and used to determine the total aerosol concentration
         (wet aerosol) in the exposure chamber. The filters were then placed in a desiccator for at least
         2 days, and reweighed. The desiccated filter mass was used to determine the H-26027 solids
         concentration.
                 2.      Particle Size Determination

         A sample to determine particle size distribution (mass median aerodynamic diameter and percent
         particles less than 10 jim diameter) was taken during each exposure with a Sierra® Series 2 10
         cyclone preseparator/cascade impactor and Sierra® series 110 constant flow air samnpler.")1

                 3.      Environmental Monitoring
                                                      0
         Chamber temperature was targeted at 22 ± V C. The temperature was monitored continually
         with a Long Stem traceable thermometer and recorded 3 times during each exposure. Chamber
         relative humidity was targeted at 50 ±:20%1. The relative humidity was measured with an Omega
         model RH5100C digital psychrometer and recorded 3 times during each exposure. Chamber
         airflow was set at the beginning of each exposure to achieve at least 10 air changes per hour.
         The airflow was monitored continually with a Brooks model 0 154 flow controller and recorded
         initially and whenever changes were made during each exposure. Chamber oxygen
         concentration was targeted to be at least 19%. The oxygen concentration was measured with a
         Biosystems model 3 100R oxygen analyzer and recorded 3 times during each exposure.

O   F.           Anatomic Pathology Evaluation

         To determine a no-observable-effect level for histopathology changes in the respiratory tract rats
         exposed to 5 10 mg/rn total H-26027 and less had the lungs, trachea, pharynxllarynx, and nose
         grossly examined I or 14 days postexposure. Subsequently, target tissues were processed for
         histopathology and microscopically examined (Table 2).




                                                             -   10-
H-26027. Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopatbology Examination of the Respiratory Tract                          DuPont- 13654



                                   RESULTS AND DISCUSSION

A.      Exposure Conditions
        (Table 1)
Animals were exposed to H-26027 aerosols at mean total concentrations of 1.0, 10, 110, 120,
  510, 1000, or 5900 mgr 3 (wet), which corresponded to mean H-26027 solids concentrations of
0.95, 10, 94, 110, 410, 560, or 940 mg/rn 3, respectively. The atmospheres generated at
510 mg/rn3 total wet H-26027 and less were considered to be respirable. demonstrating mass
median aerodynamic diameters (MMAD) ranging from 0.67 to 2-1 Rm. The atmospheres
generated at 1000 mg/rn 3 and greater were considered to be marginally respirable with MMADs
ranging from 3.5 -5.1 .rm (Table 1).

Chamber temperature ranged from 19 to 25"C, chamber relative humidity ranged from 50 to
95%, chamber airflow ranged from 18 to 19 [1mm, and the oxygen concentration was 2 1%
Although chamber temperature and relative humidity were outside the targeted parameters, these
deviations were considered not to have adversely affected the validity of this study.

B.      Mortality, Clinical Signs, and Body Weights

Deaths occurred at wet H-2602 7 aerosol concentrations of 5 10, 1000, or 5900 mg/rn' (410, 560,
or 940 mg/rn' solids, respectively). Rats died within 2 days of exposure.

Following exposures, when the rats were removed from the restrainers, the clinical signs of
toxicity frequently observed included colored discharge from either the eyes or nose. The
colored discharge was resolved by 2 days postexposure in all test groups.
Weight losses were observed following exposure. Within I day of exposure, weight losses
typically ranged from I to 12 percent of initial body weight. Surviving rats subsequently
resumed normal weight gains (approximately 5- 10 g/day) and gamned weight for the remainder of
the recovery period.

C.     Anatomic Pathology Evaluation
       (Table 2, Appendix B)

Microscopic changes were present in the larynx of rats exposed to 10 to 120 mg/rn 3 H-260217.

In the 120 mg/rn 3 group sacrificed 24 hours following a single exposure, laryngeal changes were
characterized by degeneration and necrosis of the ventral laryngeal mucosa which was lined by
attenuated epithelium. In some animals, focal areas of squamous differentiation and/or
ulceration were also present. Mild to moderate inflammation. as well as necrosis of cartilage
dorsal to the laryngeal saccule, was present in the submucosa Changes in the 10 mg/rn grou
214 hours after a single exposure were limited to slight inflammation in the submucosa in 3/5 rats.
Since slight inflammation of the laryngeal submucosa may be seen in controls, it is uncertain if
these slight changes at 10 mg/in' represent compound-related effects
    H-26027. Inhalation Approximate Lethal Concentration JALC) in Male Rats
    Including Histopathology Examination of the Respiratory Tract                          DuPont-I 3654

    Compound-related changes in the larynx of rats sacrificed following 14-days recovery were
    limited to residual cartilage necrosis in rats exposed to 110 mg/rn. All other changes observed
    (such as airway hemorrhage, low incidences of minimal laryngeal inflammation) were consistent
    with findings commonly seen in rats on inhalation studies and thus were not considered
    compound-relatedL

    No compound-related microscopic changes were present in the larynx of rats exposed to
    I mg/rn 3.

    Degeneration/necrosis of the tracheal mucosa was observed in one rat in the 120 mgrn group
    sacrificed 1 day after exposure. There were no other compound-related effects in respiratory
    tissue at any of the exposure concentrations tested.

            I.      Conclusions

    Under the conditions of this study, the NOAEL for pathology was I mg/rn. This NOAEL is
    based on minimal and equivocal findings in the larynx of rats exposed to 10 mg/rn 3. More
    definitive and more severe laryngeal changes were observed in rats exposed to 1 10/ 120 mg/in 3.
    Except for residual cartilage necrosis, laryngeal changes were reversible following 14 days
    recovery from a single exposure.


                                               CONCLUSIONS
*   Under the conditions of this study, the approximate lethal concentration (ALC) for H-26027 is a
    wet aerosol of 510 mg/rn H-26027 (410 mg/Wn solids). Since numerous exposure
    concentrations were utilized in this study, and there was a steep slope in the concentration-
    response curve between the lethal and non-lethal concentrations, a regression analysis was
    performed and an LC ) of 3 80 mg/in3 total wet aerosol (120 - 680 Mg/rn 3 95% confidence
                          50
    interval) and an LC 5o of 300 mg/rn3 H-26O27solids (9-5 - 450 mg/rn 95% confidence interval)
    was calculated. According to Hiaskell Laboratory toxicity classifications, H-26027 is considered
    to be moderately toxic (ALC 200-800 mg/rn 3 ) in male rats.

    The no observable effect level for histopathology changes is 1.0 mg/rn H-26027.

                                  RECORDS AND SAMPLE STORAGE

    Specimens (if applicable), raw data, and the final report will be retained at Haskell Laboratory.
    Newark, Delaware, or at Iron Mountain Records Management, Wilmington, Delaware.

                                                REFERENCES

    1. Calculation described in Sierra Instruments, Inc., Bulletin 7-79-2191M, Instruction Manual:
       Series 210 Amnbient-Cascade Impactors and Cyclone Preseparators.




                                                       -   12-
H-26027- Inhalation Approximate Lethal Concentration 1ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract             DuPonti13654




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                                          PE4
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract             DuPont-I 3654




                                             APPENDICES




                                                   -22
H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract             DuPont-13654




                                             APPENDIX A

                                        Determination of LC5;




                                                   -   23   -
H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                                       DuPont- 13654


                          Determination of LC50 - Wet Aerosol Concentrations

Scale of Concentration is Transformed to LGo

                Concentration            Log         Numwber          Number
                    (mg/L)          Concentration    Exposed         Responding    Rate    Probit
                    0.0010            -3.0000          El.               0.       0 0001   1.2809
                    0-0100            -2.0000          5.                0        0.0001   1.2809
                    0.1100            -0.9586          G.                0-       0.0001   1.2909
                    0.5100            -o 2924          6.                5        0-8333   5.9674
                    1l0000             0.0000          6.                5-       0-9333   5.9E74




                                                Control Sample Size      0,  =
                                                  Control Responses      0.  =
                                          Spontaneous Response Rate          =
                                                                         0.00000
                                               Heterogeneity Factor      1 0000
                                                                             =
                                                   Number of Points      5   =
                                                 Degrees of Freedom    - 3
                                                            Deviate    =1,9600
                                                                    G  = 0 5445
                                                    Number of Cycles   = 7
                                                            Average X  = -0 3242
                                                            Average Y1 = 5.3366
                                                            Average V  = 0.0000
                                                                Slope  = 3-3759
                                                         Lower Limit   = 0.8848
                                                         Upper Limit   = 5.8671
                                                            Intercept  = 6=4314
                                                         CHIi Squared  = 1.6323



                                          Concentration           951 Confidence Limits
                                Point         lmcr/L)               Lower       Upper
                            P    = 0.01       0 0771                o.0004      0.1847
                            P    = 0.05       0 1227                0-0024      0-2496
                            P    = 0.10       0.1572                0.0060      0.2960
                            P    = 0 20       0-2122                0.0177      0-3712
                            P   = 0.50        0.3767                0.1201      0.6821
                            P   = 0-80        0-6688                0 3855      2 6432
                            P    = 0.90       0.3029                0.5285      7 2009
                            P    = 0-95       1-1568                0-6490     17.4068
                            P   = 0.99        1.8411                0.9034     96.2505




                                                     -   24   -
H-26027. [nhalation Approximate Lethal Concentration IALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                                   DuPont-13654



                          Determination of LCso - Dry Aerosol Concentrations

Scale of Concentration is   Transformed to LOGic

                Concentration         Log          Number         Number
                   (mg/L)        concentration     Exposed      Vesponding     Pate    Probit
                  0.00095           -3.0223          5.             0-        0.0001   1.2809
                   0.0100           -2.0000          5.             0.        0.0001   1.2809
                   0.0940           -1.0269          6.             0.        0.0001   1.2809
                   0.4100           -0-3872          6.             5-        0.8333   5.9674
                   0.5600           -0 2518          6.             5.        0.8333   5.9674




                                             Control Sample Size        =    0.
                                               Control Responses        =    0.
                                       Spontaneous Response Rate        =    0.00000
                                            Heterogeneity Factor        =    1.0000
                                                Number of Points        =    5
                                              Degrees of Freedom        =    3
                                                         Deviate        =    1.9600
                                                               G        =    0.8246
                                                Number of Cycles        =    8
                                                       Average X        =    -0.3872
                                                       Average Y        =    5.6482
                                                       Average V        =    0.0000
                                                           Slope        =    4,6393
                                                     Lower Limit        =    0.4265
                                                     Upper Limit        =    8.8521
                                                       Intercept        =    7.4443
                                                     CI Squared         -    0.6099



                                      Concentration          951 Confidence Limits
                            Point         (mg/L)              Lower         Upper
                          P =0 01         0-0937              0 0000        0.2010
                          P W 0.05        0.1314              0.0000        0.2443
                          P = 0.10        0-1574              0.0000        0.2725
                          P = 0.20        0.1958              0.0001        0.3139
                          P = 0.50        0.2973              0.0095       0.4494
                          P = 0.80        0.4514              0 2391       2.1019
                          P = 0-90        0.5615              0-3'06       16.3473
                          P = 0.95        0.6725              0.4455      106 3359
                           P= 0.99        0.9431              0-5701     3930 0230




                                                   -25   -
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                DuPont-13654




                                             APPENDIX B

       Incidences of Gross Observations in Male Rats with Individual Animals Listed




                                                   -26-
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                                                    DuPont- 13654


           Incidences of Gross observations in Male Rats with Individual Animals Listed
                                               Day 1


                                                                             I LESION INCIDENCE (Numeric)]
                                                    -------------------------------------------------------------- I
     LESIONS                                       ITREATMENT 1 0 I1.0                     1    10     I120
                                                                     I mg/mfl (wet)                   I (wet)   I (wet) I
                                                                                     I I0.95s         1   10    1 110
                                                                                 I     I(Solids)      j(Solids) j(Solids)
                                                                  I Img/mi                              mg/mi     mg/m    I
 ------------------------------------------------------------------------------------                                        I
  LONGS                                                              I(2) I                     (5)   I    (5)   I     (6)
     NO ABNORMALITY DETECTED                                         I101             I        301    I    1          19
                                                                       .102                    302         2          20
                                                                         I             I       303    I    3          21
                                                                         I             I       304    J    4          22
                                                                         I             I       305         5          23
                                                                                       I I            I          I    24 j

 TRACHEA                                                             I(                         (5)   I    (5)   I     (6)
     NO ABNORMALITY DETECTED                                           101            I        301    I    1     1    19
                                                                       102                     302         2          20
                                                                         I            I        303    I    3     I    21
                                                                                               304    I    4          22
                                                                             I         1       305         5          23


 IPHARYUX/LARYNX                                                     I()                        (5) I      (5)   I     (6)
     NO ABNORMALITY DETECTED                                         I101              1       201  I      1     I    13     I
                                                                       102                     302  j      2     j    20
                                                                        I303                          I    31         211
                                                                                               304         4          22
                                                                                           I   305         5          23
                                                                                       I I            I          I    24

 NOSE                                                                I()                        (5)              I     ()I
                                                                                                                       165
     No ABNORMALITY   DETECTED                                       I101              1       301    I    1          19
                                                                      I102             j       302         2          20
                                                                        I              I       303    I    3          21
                                                                        I              j       304         4     I    22
                                                                         I            I        305         5          23
                                                                                       I I            I          I    24


Figure in parentheses is the number of animals grossly examined for this tissue
The absence of a number indicates the finding specified was not identified




                                                   -   27 -
H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathologv Examination of the Respiratory Tract                                                  DuPont- 13 654


            Incidences of Gross Observations in Male Rats with Individual Animals Listed
                                               Day 14



                                                                              I LESION INCIDENCE (Numeric)l
                                                    --------------------------------------------------------------- I
   I   LESIONS                                     ITREATMENT        1 0      I1.0                   10        I110
                                                                     I mg/in'l (wet)              I(wet)       I   (wet)
                                                                           I I 0.95               1 10         1    94
                                                                               I   I (Solids)     I (Solids)   I (Solids)   I
                                                                     I             I   mg/rn'     I mg/m3      I mg/rn'
 ------------------------------------------------------------------------------I
  LUNGS                                                              I(2)                   (5)   I     (5)           16)
   1   NO ABNORMALITY DETEC-TED                                           103              306    I    6             13
                                                                          104              307         7             .14
                                                                                           308         B             15
                                                                           I       I       309          9            1.6
                                                                                       I   310    I     10            17


 ITRACHEA                                                             I(2) I                (5)   I     (5)           16)
       NO ABNORMALITY DETECTED                                            la13             306          6             13
                  II104                                                                    307          7      I     141
                    II                                                             I       308          a             15
                                                                                       I   309          9             16
                                                                           I       I       310          10     I      17
                                                                                    I I           I            I      18    1
  PHARNX/LARYNXj                                                          (2)                (5)I       (5)           (6)
     9O ABNORMALITY DETECTED                                          1 103        I       306 I       6    I         13
                                                                         I104      I       307         7    j         14I
                                                                           I       I       308          a             is1
                                                                                   1       309 J        9      1      16
                                                                           I       I       310 I        10     I      17


 INOSE                                                                I(2)j                 (5)   I     (5)~          (6)
       NO ABNORMALITY DETECTED                                            103              306    I     6      I      13
                                                                          104              307          7             14
                                                                                       I   308          8             is1
                                                                                           309          9             16
                                                                           I       I 310                10            17
                                                                                    I I           I            I      18
 ------------------------------------------------------------------------
Figure in parentheses is the number of animals grossly examined for this tissue
The absence of a number indicates the finding specified was not identified




                                                   -28   -
    H-26027, Inhalation Approximate Lethal Concentration (ALC) in Male Rats
    Including Histopathology Examination of the Respiratory Tract                DuPont-13654




                                                 APPENDIX C

0      Incidences of Microscopic Observations in Male Rats with Individual Animals Listed




                                                       -   29   -
          14




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H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract             DuPont-13654




                                             APPENDIX D

                                 Individual Animal Pathology Data




                                                   -35-
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                  DuPont- 13654


                                            Individual Animal Pathology Data

Coricentration    0,0 fig/rn'         Sex;     Males

Animal Ref            Microscopic & Macroscopic Findings

101                  Terminal Sacrifice
                     Killed on Day :1
                     Exposure Group :Day 1
                     Animal is       signed of f   from necropsy

       Gross Pathology:

                 No Macroscopic Abnormality Observed
                     LUNGS,* TRACHEA,* PHARYNX /LARYNX. NOSE

       Histopathology

                 N~oMicroscopic Abnormality Observed
                     PHARYNX/LAP.Y4-

102                  Terminal Sacrifice
                     Killed on Day    1
                     Exposure Group   Day 1
                     Animal is signed of f from necropsy

       Gross Pathology

                 No Macroscopic Abnormality observed
                     LUNGS,* TRACHEA, PHARYNX/LARYNX, NOSE

       Histopathology    -

                 No microscopic Abnormality Observed
                     PHARYNE, LARYNX

103                   Terminal Sacrifice
                      Killed on flay   14
                      Exposure Group           Day 14
                     Animal     is   signed off from necropsy

       Gross Pathology

                 No Macroscopic Abnormality observed
                     LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE


103 Continued on the next page          .




                                                         -36-
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including H-istopathology Examination of the Respiratory Tract            DuPonti 36 54


                                       Individual Animal Pathology Data

Concentration:     0.0 mq/rn'       Sex:   Males

Animal Ref            microscopic & macroscopic Fi.ndi.ngs

103                   Continued from previous page

      HIstopathology

                 PHARYNX /LARYNX
                      HE14ORRHAGE

104                   Terminal Sacrifice
                      Killed on Day   14
                      Exposure Group   Day 14
                      Animal is signed off from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PHARYNX/ LARYINX, NOSE

      Histopathology

                 PHARINX/LARYNX
                      HEMORRHAGE




                                                    -   37 -
U{-26027. Inhalation Approximate Lethal Concentration I(ALC) in Mate Rats
Including lHistopathology Eicaniination of the Respiratory Tract               DuPont-I 3654



                                     Individual Animal Pathology Data

Concentration.- 1.0 mg/r)     (Wet). 0.95 mg/rn'   (Solids)     Sex:   Males

Animal Ref           Microscopic & Macroscopic Findings

301                  Terminal Sacrifice
                     Killed on Day   I
                     Exposure Group :Day 1
                     Animal in signed off from necropsy

      Gross Pathology

                No Macroscopic Abnormality Observed
                   LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE

      Histopathology

               PHARYNX/ LARYNX
                    HEMORRHAGE

302                  Terminal Sacrifice
                     Killed on Day   I
                     Exposure Group   Day 1
                     Animal is signed of f from necropsy

      Gross Pathology

                No Macroscopic Abnormality Observed
                    LUNGS, TRACHEA. PHARYNX/LAPYNX, NOSE

      Histopathology

                No Microscopic Abnormality Observed
                    PHARYN'X/ LARYNX

303                  Terminal Sacrifice
                     Killed on Day :1
                     Exposure Group   Day 1
                     Animal is signed of f from necropsy

      Gross Pathology

                No Macroscopic Abnormality Observeda
                    LUNGS, TRACHEA, PHARYNX/ LARYNX, NOSE


303 Continued on the next page




                                                     -38-
H-26027' Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopatbology Examination of the Respiratory Tract                         DuPont- 13654



                                     Individual Animal Pathology Data

Concentration-     1 0 mg/r'   ~wetK. 0-95   mg/rn'   (Solids)         Sex-   Males

Animal Ref            microscopic & macroscopic Findings

303                   Continued from previous page

      Hi.stopathiology

                 PHARYNX/LARYWC
                      HEMORRHAGE.

304                   Terminal Sacrif ice
                      Killed on Day    1
                      Exposure Group    Day 1
                      Animal is signed off from necropsy

      Gross Pathology     -

                 No Macroscopic Abnormality Observed
                    LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE

       Histopathology

                 PHARYNK, LARYNX
                      HEMORRHAGE.

305                   Terminal Sacrifice
                      Killed on Day t1
                      Exposure Group ;Day 1
                      Animal is signed off from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PIARYNX/LARYNX. NOSE

      Histopathology

                 PHAPYNX/t.APYNX
                      TISSUE UNSATISFACTORY/LOST        -NOT       PRESENT IN BLOCK




                                                        -39    -
H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                       DuPorit-i3654


                                       Individual Animal Pathology Data

Concentration-    1.0 mg/m2   tWetl.     0.95 mg/ml   (Solids$       Sex-   Males

Animal Ref           microscopic & macroscopic Findings

306                  Terminal Sacrifice
                     Killed on Day    14
                     Exposure Group    Day 14
                     Animal is signed of f from necropsy

      Gross Pathology

                 No macroscopic Abnormality Observed
                     LUNGS, TRACHEA. PHARYNX /LARYNX, NOSE

      Histopathologyt

                 No Microscopic Abnormality Observed
                    PHARYNX/LARYIM

307                  Terminal Sacrifice
                     Killed on Day i14
                     Exposure Group :Day 14
                     Animal is signed off from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PHARYNX/LARYUX, NOSE

      Histopathology

                 No Microscopic Abnormality Observed
                     PRARYNX/LARY4X

308                  Terminal Sacrifice
                     Killed on Day    14
                     Exposure Group    Day 14
                     Animal is signed off from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS,   TRACHEA,    PHARYNX /LARYNX.    NOSE


308 Continued on the next page




                                                       -40-
H-26027. Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathologv Examination of the Respiratory Tract                 DuPont- 13654


                                      Individuial Animal Pathology Data

concentration-     1.0 mg/rn'   (Wet). 0.95 mg/ma   (Solids)   Sex:   Hales

Animal Ref            Microscopic & Macroscopic Findings

305                   Continued from previous page

      Histopatbology

                 PHARYNI /IARYHX
                      HEMORRHAGE

309                   Terminal Sacrifice
                      Killed on Day   14
                      Exposure Group   Day 14
                      Animal is signed of f from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed -
                     LUNGS, TRACHEA, PHAR.YNX/LARYME, NOSE

      Histopathology

                 PHARYNX /LAPYNX
                      HEMORRHAGE.

310                   Terminal Sacrif ice
                      Killed on flay   14
                      Exposure Group    Day 14
                      Animal is signed off from necropsy

      Gross Pathology

                 No macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PHARYNX/LARYNA, HOSE

      Histopathology

                 PHARYNX/LARYNX
                      HEMORRHAGE




                                                     -41-
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                     Du~ont-13654


                                            Individual Animal Pathology Data
                              3
Concentration-     10 mg/rn       (wet),   10 mg/rn'   (Solidsl    Sex:   Males

Animal Ref               microscopic & M~acroscopic Findings

                         Teirminal Sacrif ice
                         Killed on Day -1
                         Exposure Group     Day 1
                         Animal is signed off from necropsy

         Gross Pathology

                  No Macroscopic Abnormality Observed
                      LUNGS, TRACHEA. PHARYNX/LARYNC, NOSE

         Histopathology -

                 PHARYNX/LAPYIJX -
                      HEMORRHAGE,
                      INFLAMMATION, SUBACUTE/CHRONIC, minimal-
                 NOSE I & II
                      EXUDATE. NASAL CAVITY. minimal.
                      HYPERPLASIA, EPITHELIUM, RESPIRATORY, minimal.

                  No Microscopic Abnormality Observed
                      LUNGS, TRACHEA, NOSE III & IV

     2                   Terminal Sacrif ice
                         Killed on Day i1
                         Exposure Group :Day 1
                         Animal is signed off from necropsy

         Gross Pathology

                  No Macroscopic Abnormality Observed
                      LUNGS, TRACHEA, PHARYWX/LARYNX, NOSE

         Histopathology

                 LUNGS
                        INFLAMMATION, SUBACUTE/CHRONIC, minimal.
                 PHAP.YNX/ LARYNXz
                       HEMORRHAGE.
                        INFLAMMATION, SUBACUT-rE/CHRONIC, minimal

                  No microscopic Abnormality Observed
                      TRACHEA, NOSE I &II,  NOSE III &IV




                                                            -42-
H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                    DuPont-13654



                                        Individual Animal Pathology Data

Concentration.     10 ing/m'   JWet),   10 mg/rn'   (Solids)       Sex   Males

An~imal Ref           Microscopic & Macroscopic Findings

     3                Terminal Sacrifice
                      Killed on Day   I
                      Exposure Group   Day 1
                      Animal is signed of f from necropsy

         Gross Pathology

                  No Macroscopic Abnormality Observed
                      LUNGS, TRACHEA, PHi RYNX/LARYNX, NOSE

         Histopathology

                 NOSE III & IV
                      HEMORRHAGE.

                  No Microscopic Abnormality Observed
                      LUNGS, TRACHEA, PHARYNX/LARYNXf NOSE I &II

4                     Terminal Sacrifice
                      Killed on Day   1
                      Exposure Group   Day 1
                      Animal is signed off from necropsy

         Gross Pathology

                  No Macroscopic Abnormality Observed
                      LUNGS, TRACHEA, PHARXIIX/LAR XNX, NOSE

         Histopathology;

                 PHARYNX/LARYNX
                      HEMORRHAGE

                  No Microscopic Abnormality Observed
                      LUNGS, TRACHEA. NOSE I & II. NOSE III &IV

     S                Terminal Sacrifice
                      Killed on Day    I
                      Exposure Group    Day 1
                      Animal is signed off from necropsy

         Gross Pathology:

               No Macroscopic Abnormality Observed
                   LUNGS, TRACHEA. PHARYNX/ LARYNX. NOSE
5 Continued on the next page     -




                                                         -43   -
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopatbology Examination of the Respiratory Tract                    DuPont-I 3654



                                       Individual Animal Pathology Data

Concentration.     10 mg/rn'   (Wet). 10 mg/rn'   (Solids        Sexm    Males

Animal Ref            Microscopic a Macroscopic Findings

5                     Continued from' previous page

      Histopathology

                 PHARYNI /LARYNX
                      HEMORRHAGE.
                      INFLAMMATION,    SUBACUT-TE/CHRONIC,    minimal.

                 No Microscopic Abnormality observed
                     LUNGS, TRACHEA, NOSE I & II, NOSE III &IV

6                     Terminal Sacrif ice
                      Killed on Day -14
                      Exposure Group    Day 14
                      Animal is signed off from necropey

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PHARYNI/LARYNX, NOSE

      Histopathology

                 PHARYNI, LARYNX-
                      HEMORRHAGE.

                 No Microscopic Abnormality observed
                     LUNGS, TRACHEA, NOSE I & II, NOSE III &IV

7                     Terminal Sacrifice
                      Killed on Day -14
                      Exposure Group :Day 14
                      Animal is signed off frow necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE




7 Continued on the next page




                                                       -44-
H-26027; Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                   DuPont-13654


                                       Individual Animal Pathology Data

Concentration-     10 mg/rn'   (Wet , 10 mg/rn'   (Solids)       Sexi   Males

Animal Ref            Microscopic & Macroscopic Findings

7                     Continued from previous page

      Histopathology

                 PHAX/LAMYNX
                     HEMORRHAGE.

                  No Microscopic Abnormality observed
                      LONGS, TRACHEA. NOSE I & II, NOSE III & IV

8                     Terminal Sacrifice
                      Killed on Day   14
                      Exposure Group   Day 14
                      Animal is signed of f from necropsy

      Gross Pathologyv

                  No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE

      Histopathology

                 PHARYNX/LARYNX
                      HEMORRHAGE -

                  No Microscopic Abnormality observed
                      LUNGS, TRACHEA, NOSE I & II, NOSE III &IV

9                     Terminal Sacrifice
                      Killed on Day   14
                      Exposure Group   Day 14
                      Animal is signed off from necropsy

      Gross Pathology

                  No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA. PHASYUX/LARYNX, NOSE

      Histopathology

              PHARYNX/ LARYNX
                   HEMORRHAGE -
9 Continued on the next page




                                                       -45   -
H--26027, Inhalation Approximate Lethal Concentration 4ALC) in Male Rats
Including Histopathology Examnination of the Respiratory Tract                      DuPont- 13654


                                       Individual Animal Pathology Data

Concentration:     10 mg/rn'   (wet), 10 mg/mi   (Solids)          Sex.     Males

Animal Ref            Microscopic & Macroscopic Findings

9                     continued from previous page

      Histopathology

                 No Microscopic Abnormality Observed
                     LUNGS, TRLACHEA, NOSE I & II, NOSE iir &ivl

10                    Terminal Sacrif ice
                      Killed on Day -14
                      Exposure Group    Day 14
                      Animal is signed off from necropsy

      Gross Pathology:

                 No Macroscopic Abnormality Observed
                     LUNGS,* TRACHEA, PHARYNX/LARYNX, NOSE

      Histopatliology

                 PHARYNX/LARYNX
                      HEMORRHAGE.
                      INFLAMMATION,    SUBACUTE/CHRONIC,        minimal-

                 No Microscopic Abnormality Observed
                     LUNGS, TRACHEA, NOSE I &II,  NOSE III            &IV




                                                      -46   -
H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Exarmnation of the Respiratory Tract                      DuPont- 13654,



                                         Individual Animal Pathology Data

Concentration:     110 mg/1n3   (Wet),   94 mg/ml   (Solids)   Sex:        Males

Animal Ref            microscopic a macroscopic Findings

13                    Terminal Sacrifice
                      Killed on Day   14
                      Exposure Group :Day 14
                      Animal is signed of f from necropsy

      Gross Pathology

                  No Macroscopic Abnormality Observed
                      LUNGS. TRACHEA. PHARYNX/LARYNX. HOSE

      Histopathology

                 PHARYNXf LARYNX
                      HEMORRHAGE
                      NECROSIS. CARTILAGE.

                  No Microscopic Abnormality Observed
                      LUNGS, TRACHEA, NOSE I & II, NOSE III      &IV

14                    Terminal Sacrifice
                      Killed on Day    14
                      Exposure Group    Day 14
                      Animal is signed off from necropsy

      Gross Pathology:

                  No Macroscopic Abnormality Observed
                      LUNGS, TRACHEA, PH[ARYNX/LARYNX. NOSE

      Histopathology

                 PIIARY1TX/LARYNX
                        HEMORRHAGE.
                        NTECROSIS, CARTILAGE,

                  No Microscopic Abnormality Observed
                      LUNGS, TRACHEA. NOSE I &II.  NOSE III           IV




                                                        -47-
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                          DuPont-I 3654


                                            Individual Animal Pathology Data
                               3
concentration-     110 mo/rn       (Wet),   94 rng/ml   (Solids)       Sex.-   Males

Animal Ref            Microscopic       & Macroscopic Findings

15                    Terminal Sacrifice
                      Killed on Day   14
                      Exposure Grouip  Day 14
                      Animal is signed off from necropsy

      Gross   Pathology

                  No Macroscopic Abnormality observed
                      LUNGS, TRACHEA, PHARYNX /LARYNX. NOSE

      HIstopathology

                 PHARYNX/LARYNX
                      NECROSIS, CARTILAGE.

                  No Microscopic Abnormality observed
                     LUNGS, TRACHEA, NOSE I & II.  NOSE III &IV

16                    Terminal Sacrifice
                      Killed on Day   14
                      Exposure Group   Day 14
                      Animal is signed of f from necropsy

      Gross Pathology

                  Ilo Macroscopic Abnormality Observed
                       LUNGS, TRACHEA, PHARYNX /LARYNX. NOSE

      Histopathology

                 PHARYNX/LARYrA
                      HEMORRHAGE.
                      INFLAMMATION, SUBACUTEYCHRONIC,              minimal.
                      NECROSIS, CARTILAGE=
                 NOSE III & IV
                      HEMORRHAGE.

                 No Microscopic Abnormality Observed
                     LUNGS, TRACHEA. NOSE I &II




                                                            -48-
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                              DuPont-13654



                                        Individual Animal Pathology Data

Concentration:     110 mg/m3   (Wet),     94 mg/m3   (Solids)              Sex:    Males

AnimalRef .....       microscopic & Macroscopic Findings

11                    Terminal Sacrifice
                      Killed on Da    14
                      Exposure Group :Day 14
                      Animal is signed off from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA. PHARYNX/LARYNX. NOSE

      Histopathology:

                 PHARYNX /LARYNX
                      HEMORRHAGE.
                      NECROSIS. CARTILAGE.

                 No Microscopic Abnormality observed
                     LUNGS, TRACHEA, NOSE I r. 11, NOSE III                  &IV

18                    Terminal Sacrifice
                      Killed on flay  14
                      Exposure Group   Day 14
                      Animal is signed of f from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                      LONGS,   TRACHEA,     PHARYNX/LARYINx,        NOSE

      Histopathology

                 PHARYNX/ LARYINX
                      HEMORRHAGE.-
                      NECROSIS,* CARTILAGE,

                 No Microscopic Abnormality Observed
                     LUNGS, TRACHEA. NOSE I &II,  NOSE III &IV




                                                         -49    -
H-26027: Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                   DuPont-I 3654


                                       Individual Animal Pathology Data

Concentration:     120 mg/-'    (wet), 110 nig/un3   (Solids)     Sex.- Males

Animal Pef               Microscopic & Macroscopic Findings

19                       Terminal Sacrifice
                         Killed on Day . 1
                         Exposure Group -Day 1
                         Animal is signed off from necropsy

      Gross Pathology

                  No Macroscopic Abnormality observed
                      LUNGS, TRACHEA, PHARYNX/L.ARYM, NOSE

      Histopathology

                 LUNGS
                      INFLAMMATION, SIJEACTITE/CHRONIC, minimal.
                 PHAPYNX/ LARYNX
                      INFLAMMATION, ACUTE, mild
                      NECROSIS,* CARTILAGE
                      HEMORRHAGE.
                      DEGENP.ATION/NECPOSIS, MUCOSA, VENTRAL, moderate

                  No microscopic Abnormality Observed
                      TRACHEA, NOSE I & II, NOSE III & IV

20                       Terminal Sacrifice
                         Killed on Day -1
                         Exposure Group : Day 1
                         Animal is signed of f from necropsy

      Gross Pathology

                  No Macroscopic Abnormality observed
                      LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE

      Hiatopathology

              PHARYNX/ LARYNX
                   HEMORRH{AGE.
                   INFLAMMATION, ACUTE, mild-
                   NECROSIS, CARTILAGE
                   DEGENERATION/NECROSIS, MUCOSA, VENTRAL, mild.
              NOSE I & II
                   HEMORRHAGE-
20 Continued on the next page




                                                        -   50-
H-2 6027 Inhalation Approximnate Lethal Concentration (A.LC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                       DuPont- 13654



                                          Individual Animal Pathology Data

Concentration:     120   Mg/M3   (Wet),   110 mg/mli   Solids)        Sex.- Males

Animal Ref               Microscopic & Macroscopic Findings

20                       Continued from previous page

       Histopathology

                 NOSE III & IV
                      HEMORRHAGE.

                  No Microscopic Abnormality Observed
                      LUNGS, TRACHEA

21                    Terminal Sacrifice
                      Killed on Day   1
                      Exposure Group   Day 1
                      Animal is signed off from necropsy

       Gross Pathology

                  No Macroscopic Abnormality Observed
                      LUINGS, TRACHEA, PHARY14X/LARYNX, NOSE

       Histopathology

                 PHARYIIX/LARYNX
                      HEMORRHAGE.
                      INFLAMMATION, ACUTE, mild
                      NECROSIS, CARTILAGE.
                      DEGENERATION/NECROSIS  MUCOSA,            VENTRAL,   mild.

                  No Microscopic Abnormality Observed
                      LUNGS, TRACHEA, NOSE I & II, NOSE III &IV

22                       Terminal Sacrifice
                         Killed on Day    1
                         Exposure Group :Day 1
                         Anim~al is signed of f from necropsy

       Gross Pathology

                  No Macroscopic Abnormality Observed
                      LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE

22 Continued on the next page




                                                         -51-
H-26027- Inhalation Approximate Lethal Concentration (ALC) in Male Rats
Including Histopatbology Examination of the Respiratory Tract                     DuPont-13654


                                        Individual Animal Pathology Data

Concentration!     120 mg/m3   (Wet),   110 ing/m3   (Solids)     Sex:    Males

Animal Ref            Microscopic & Macroscopic Findings

22                    Continued from previous page

      Histopathology

                 PHARYNX/LARYNX
                      HEMORRHAGE.
                      INFLMMATION, ACUTE, mild.
                      NECROSIS, CARTILAGE-
                      DEGENERATION/NECROSIS, MUCO-SA,       VENTRAL,   mild
                 NOSE III & IV:
                      HEMOPRRHAGE

                 No Microscopic Abnormality Observed
                     LUNGS. TRACHEA, NOSE I & II

23                    Terminal Sacrifice
                      Killed on Day   1
                      Exposure Group :Day 1
                      Animal is signed off from necropsy

      Gross Pathology

                 No Macroscopic Abnormality Observed
                     LUNGS, TRACHEA, PHARYNX/LARYNX, NOSE

      Histopatholocry

                 TRACHEA:
                      DEGENERATION/NECPOSIS, MUCOSA, moderate.
                 PHARYNX/LARYNX.
                      HEMORRHAGE.
                      INFLAMMATION, ACUTE, moderate.
                      NECROSIS, CARTILAGE.
                      DEGENERATION/NECROSIS, MUCOSA, VENTRAL, moderate.

                  No Microscopic Abnormality Obser-ved
                      LUNGS, NOSE I &II,  NOSE III 5.IV




                                                        -52-
H-26027-, Inhalation Approximate Lethal Concentration 4ALC) in Male Rats
Including Histopathology Examination of the Respiratory Tract                           DuPont- 13654


                                         Individual Animal Pathology Data

concentration;     120 mg/nil   (wet),   110 mg/is'    (solids)       Sex.    Males

Animal Ref            Microscopic & Macroscopic Findings

24                    Terminal Sacrifice
                      Filled an Day   1
                      Exposure Group   Day 1
                      Animal is signed off from necropsy

      Gross Pathiology

                 No Macroscopic Abnormality observed
                     LUNGS, TRACHEA, PHARYNX/IARYNX, NOSE

      Histopathology

                 PHARYNX /LARYNX
                      INFLAMMATION, ACUTE, moderate-
                      NECROSIS, CARTILAGE.
                      DEGENEPRATION/NECYIOSIS,        MUCOSA,   VENTRAL,   mioderate-

                 No Microscopic Abnormality Observed
                     LUNGS, TRACHEA, NOSE I   11I,NOSE III &IV




                                                           -53-
7001 2510 0006 9113 2343

				
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