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Individual Micropropagation Assignment MICROPROPAGATION PROTOCOL REVIEW PAPER An eight to ten (8-10) page double-spaced typed review paper (not including references) will be due on, March 1, 2011. Please note that no time extensions will be allowed. This paper will represent 15% of your final grade. You will select a specific plant for which a tissue culture propagation procedure has NOT been published.. You will search the available literature to review the micropropagation protocols relevant to the propagation of that particular plant. The instructional objectives of this exercise are for each student: 1) to become familiar with the procedures required to obtain information on specific micropropagation protocols; 2) to be able to critically analyze the published information and communicate the result of these analyses in written form and; 3) design a micropropagation protocol based on the information collected. Please be sure to have the plant species for your paper approved by Dr. Barry before proceeding.. It is expected that students will choose the same plant species for the self directed laboratory experience (see below). Please submit your species/crop name to me by February 1, 2011. You should begin by choosing appropriate key words and searching the electronic databases for published research on the chosen subject. For your review, select at least 10 original, relevant and peer reviewed (refereed) research papers. It is very important that you obtain complete copies of each original journal article. Do not rely on abstracts, research summaries, or review articles to evaluate the quality and importance of the information presented in the original paper. Review articles are useful but will not count toward the required 10 references. SIUE maintains subscriptions to many journals. As SIUE students, you can often directly print off PDF versions of more recent publications by visiting the specific journal websites. Older journal volumes can be located on the shelves by consulting the SIUE Library Catalog online. These articles will probably have to be photocopied in the library. In your paper, be sure to discuss, compare and contrast any donor plant pretreatments [Stage 0], indicate the explants used, optimal surface sterilization procedures, optimal media for Stage I [culture initiation], Stage II [propagule multiplication], Stage III [pretransplant/rooting] and Stage VI [transplant procedures]. Discuss any conflicting differences in results reported in the papers that you have read. Please be sure to correctly cite each paper by authors and date in the text. Based upon your analytical review, describe a tentative but complete micropropagation protocol that you would first attempt to use. At the end of your paper also be sure to completely list and correctly cite the references used. Note: papers not following this format will be returned without a grade. Please be sure to attach copies of the research papers you used to write your review paper. A detailed grading rubric for this assignment will be provided. Your grade will depend on organization, integration, critical analysis and clarity. Graduate student papers will be more critically reviewed. I suggest that you allow a friend (or critic) to read your paper before submission. Your paper must be free of spelling and grammatical errors. Most word processing programs have spelling and grammar checkers. However, these spell checkers are not completely reliable. SELF DIRECTED LABORATORY EXPERIENCE Students will independently attempt to successfully micropropagate a single plant species. Before starting, the species selected by each student must be approved by Dr. Barry. It is expected that the same species used for the micropropagation protocol review paper will also be used for this assignment. You will be provided some class time to work on this project and my research laboratory (SL0222) will also be open on Wednesdays from 9-11. Students will be expected to work independently without supervision. Other days and times may be available through consultation with Dr. Barry or Erin. You are responsible for preparing your own media for this exercise. Reagents, distilled water, glassware, will be available for your use. Please notify Erin at least one week in advance about specific needs and she will arrange for the materials to be available. If you require a chemical we do not currently possess, we need time to purchase the chemical or we will recommend an appropriate substitution. IMPORTANT: All other materials (media, sterile water, and glassware) prepared for use in scheduled micropropagation laboratory exercises must not be used for your individual projects. You will be required to submit a report describing the results of this self directed project in the format used for laboratory reports. This report will be due April 28 and will account for 10% of your final grade. Depending on the success achieved in completing each of the micropropagation stages, you will receive up to 4 extra credit points to your final grade. In order to receive these extra credit points, your cultures will be continually evaluated by Dr. Barry during the semester up until the last laboratory period (April 28). Extra credit points will be awarded as follows: POINTS* STAGE I: Establishment of a Sterile & Growing Culture...... +1 STAGE II: Successful Rapid Propagule Multiplication........... +2 STAGE III: Successful Rooting......................................... +3 STAGE IV: Re-establishment in Soil ............................... +4 Points will be added to the final course average achieved. Please note that to achieve points for a specific stage, the previous micropropagation stage(s) must have been completed (eg., a Stage I sterile shoot must go through shoot Stages II and III before establishment in soil).
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