Documents
Resources
Learning Center
Upload
Plans & pricing Sign in
Sign Out

ST2

VIEWS: 49 PAGES: 855

									    legend for "associations", column "G" and organelle localisations in the TAB title
A        Autophagosome
C        Cytosol, cytoplasm
D        Endosome
E        ER
G        Golgi
L        Lipid particle
M        Mito
N        Nucleus
P        PM
V        Vacuole
W        Cell wall
X        Peroxisome
U        Unknown
O        Other
R        Ribonucleoparticle
                                                                                          Associati Localisati
accession   protein   Description       MW, Da BioFun             MolFun                  ons          on         Link            TMHMM
YDL229W     SSB1                            1842 is a ribosome-associated molecular C, R
                      Cytoplasmic ATPase that protein biosynthesisATPase activity                      soluble fraction
                                                                                                                    with
                                                                                          chaperone, functionsSSB1J-protein partner0Zuo1p; may be involve
YBR177C     EHT1                            1356 lipid metabolism
                      Acyl-coenzymeA:ethanol O-acyltransferase thathydrolase activityM in medium-chain fatty acid ethyl ester 0
                                                                  serine plays a minor L,  role        Mito       EHT1                    biosynthesis; contains e
YOR317W     FAA1                            2103 synthetase with
                      Long chain fatty acyl-CoA lipid metabolism a preference for C12:0-C16:0 Mito acids;FAA1
                                                                  long-chain-fatty-acid-CoA ligasefatty
                                                                                          L, M           activity                         0
                                                                                                                    involved in the activation of imported fatty ac
YJL052W     TDH1                             999 glycolysis       glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity that catalyzes the
                                                                                          L, M, W, Ccytoplasm gluconeogenesis; tetramer
                      Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis andTDH1                                 0
YJR009C     TDH2                             999 glycolysis       glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity that catalyzes the
                                                                                          L, M, W, Ccytoplasm gluconeogenesis; tetramer
                      Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis andTDH2                                 0
YGR192C     TDH3                             999 glycolysis       glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity that catalyzes the
                                                                                          L, M, W, Ccytoplasm gluconeogenesis; tetramer
                      Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis andTDH3                                 0
YFL005W     SEC4                             648 cytokinesis
                      Secretory vesicle-associated Rab GTPase essential for exocytosis; associates withSEC4
                                                                  GTPase activity         M            Mito                               0
                                                                                                                    the exocyst component Sec15p and may reg
YPR165W     RHO1                             630 cell subfamilyGTPase activity
                                                                   of Ras-like proteins, involved establishment of cell polarity; regulates protein kinase C
                                                                                          M,
                      GTP-binding protein of the rhowall organization and biogenesis P, X inMito                  RHO1                    0
YBR078W     ECM33                           1737 cell wall organization a possible role apical Mito
                                                                  unknown                 M,                      ECM33                   2
                      GPI-anchored protein of unknown function, has and biogenesis in W, P bud growth; GPI-anchoring on the plasma membrane cru
YMR307W     GAS1                            1680 cell wall organization and biogenesis localizesMito
                                                                    for cell wall assembly; W, P, activity
                                                                                          M,                        surface               0
                      Beta-1.3-glucanosyltransferase, required1,3-beta-glucanosyltransferaseG to the cellGAS1 via a glycosylphosphatidylinositol (G
YAL005C     SSA1                            1929 protein folding ATPase activity          V, (NLS)-directed nuclear                       0
                      ATPase involved in protein folding and nuclear localization signalW, N, Ccytoplasm SSA1transport; member of heat shock protein
YOL086C     ADH1                            1047 fermentation alcohol dehydrogenase activity cytosol
                                                                                          C                        required               0
                      Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; ADH1 for the reduction of acetaldehyde to
YLR109W     AHP1                             531 regulation hydroperoxidesperoxidase activity cytoplasm AHP1
                                                                  thioredoxin to protect
                      Thiol-specific peroxiredoxin, reducesof cell redox homeostasisCagainst oxidative damage; function in vivo0requires covalent conju
YLR058C     SHM2                            1410 one-carbon compoundhydroxymethyltransferase activity SHM2
                                                                  glycine metabolism C
                      Cytosolic serine hydroxymethyltransferase, involved in one-carbon metabolism     cytoplasm                          0
YPL218W     SAR1      GTPase, GTP-binding proteinto Golgi transport component of COPII coat of vesicles; coat
                                             712 ER of the ARF family, activity
                                                                  GTPase                  G                       SAR1                    0
                                                                                                       COPII vesicle required for transport vesicle formation du
YGL200C     EMP24     Integral membrane component of endoplasmic reticulum-derived E
                                             612 ER to Golgi transport
                                                                  unknown                              ER         EMP24                   2
                                                                                          G, COPII-coated vesicles, which function in ER to Golgi transport
YKL094W     YJU3                               sequence similarity to monoglyceride lipase (MGL), localizes YJU3 particles
                      Serine hydrolase with942 unknown                                    L,
                                                                  serine hydrolase activityM, C cytoplasm to lipid                        0
YML008C     ERG6                            1152 ergosterol biosynthesis
                                                                  sterol zymosterol to fecosterol in the
                                                                                          L, M, E Mito            ERG6                    0
                      Delta(24)-sterol C-methyltransferase, converts 24-C-methyltransferase activityergosterol biosynthetic pathway by methylating pos
YBR196C     PGI1                            1665 glycolysis       glucose-6-phosphate M, C isomerasecytosol
                                                                                                         activity PGI1                    0
                      Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate;
YCR012W     PGK1                            kinase, catalyzes phosphoglycerate kinase activity groups from the
                                                                                          M, C                                             of
                      3-phosphoglycerate 1251 glycolysis transfer of high-energy phosphorylcytoplasm PGK1 acyl phosphate0 1,3-bisphosphoglyce
YDR032C     PST2                              to members          unknown
                      Protein with similarity597 unknownof a family of flavodoxin-likeM, C             cytoplasm oxidative stress in a Yap1p dependent mann
                                                                                            proteins; induced byPST2                      0
YDR050C     TPI1                             747 glycolysis       triose-phosphate mRNAC  M, half-life is
                                                                                                       cytoplasm TPI1
                      Triose phosphate isomerase, abundant glycolytic enzyme;isomerase activity regulated by iron availability;0           transcription is controll
YKL060C     FBA1                            1080 glycolysis       fructose-bisphosphate gluconeogenesis; FBA1
                                                                                          M, C         activity                           0
                      Fructose 1,6-bisphosphate aldolase, required for glycolysis and aldolase cytoplasmcatalyzes conversion of fructose 1,6 bisphosph
YPR080W     TEF1                            1377 translational elongation elongationM, C, R ribosome TEF1
                                                                  translation               factor activity                               0
                      Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA
YPR183W     DPM1      Dolichol phosphate mannose (Dol-P-Man) synthase ofglycosylation
                                             804 protein amino acid N-linked the ER M, E
                                                                  transferase activity, transferring Mito
                                                                                           membrane, catalyzes the formation of Dol-P-Man from Dol-P and
                                                                                                        glycosyl groups
                                                                                                                  DPM1                    1
YIR038C     GTT1                             705 S-transferase glutathione transferase activity Mito
                                                                   capable                M, E                    GTT1                    0
                      ER associated glutathioneglutathione metabolism of homodimerization; expression induced during the diauxic shift and throughou
YFL038C     YPT1                             621 ER to in the ER-to-Golgi step
                                                                  GTPase                  M, G, E pathway; complex formation with the Rab escort protein
                      Ras-like small GTPase, involvedGolgi transport activity of the secretoryMito                YPT1                    0
YDR342C     HXT7      High-affinity glucose1713 hexose transport facilitator superfamily, nearly identical HXT7
                                             transporter of the major
                                                                  glucose transporter activity
                                                                                          M, P         Mito                              at
                                                                                                                   to Hxt6p, expressed 11 high basal levels relat
YGL008C     PMA1                            2757 regulation of pH
                      Plasma membrane H+-ATPase, pumps protons out of the cell; major activity, phosphorylative mechanism
                                                                  hydrogen-exporting ATPase regulator of cytoplasmic pH and plasma membrane potenti
                                                                                          M, P         PM         PMA1                   10
YPL036W     PMA2      Plasma membrane H+-ATPase, isoform hydrogen-exporting ATPase activity, phosphorylative mechanism cytoplasmic pH and p
                                            2844 regulation of pH Pma1p, involved in pumping PM
                                                                  of                      M, P                    PMA2                   10
                                                                                                       protons out of the cell; regulator of
YGR266W     YGR266W                         2106 unknown
                      Protein of unknown function, predicted tounknowna single transmembrane domain; YGR266W both the mitochondrial outer mem
                                                                    contain               M, P         Mito        localized to           0
YGR254W     ENO1                            1314 glycolysis        that catalyzes the conversion ofcytoplasm ENO1
                                                                                          M, V, C
                      Enolase I, a phosphopyruvate hydratase phosphopyruvate hydratase activity                                           0
                                                                                                         2-phosphoglycerate to phosphoenolpyruvate during gly
YHR174W     ENO2                            1314 glycolysis         that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during gly
                                                                                          M, V, C soluble fraction
                      Enolase II, a phosphopyruvate hydratasephosphopyruvate hydratase activity                   ENO2                    0
YJL171C     YJL171C                         1191 unknown
                      GPI-anchored cell wall protein of unknown function; induced inM, W
                                                                  unknown                               to cell     damaging              0
                                                                                            response Mito wallYJL171C agents and by mutations in genes
YOR133W     EFT1                            2529 translational elongation elongationR
                                                                   by EFT2;                 factor activity
                                                                                                       ribosome EFT1                      0
                      Elongation factor 2 (EF-2), also encodedtranslationcatalyzes ribosomal translocation during protein synthesis; contains diphtham
YNL209W     SSB2                            1842 is a ribosome-associated molecular R, C
                      Cytoplasmic ATPase that protein biosynthesisATPase activity                      cytoplasm with
                                                                                          chaperone, functionsSSB2J-protein partner0Zuo1p; may be involve
YDL185W     TFP1                            3216 vacuolar acidification
                                                                  hydrogen-transporting nucleotide binding sites; protein precursor
                                                                                          V            vacuole TFP1
                      Vacuolar ATPase V1 domain subunit A containing the catalytic ATPase activity, rotational mechanism 0undergoes self-catalyz
YGL156W     AMS1                            3252 carbohydrate metabolism
                                                                    free oligosaccharideV  (fOS)       vacuole AMS1                       0
                      Vacuolar alpha mannosidase, involved inalpha-mannosidase activity degradation; delivered to the vacuole in a novel pathway sep
YOR086C     TCB1                             containing three
                      Lipid-binding protein3561 unknown calciumbinding bindingM
                                                                  lipid and lipid                       non-tagged protein localizes to mitochondria and GFP-f
                                                                                            domains; Mito         TCB1                    2
YFR019W     FAB1                            6837 response to stress
                                                                  1-phosphatidylinositol-3-phosphate 5-kinase
                                                                                          M, kinase that generates phosphatidylinositol (3,5)P2, which is inv
                      1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membraneV, D vacuole, Mitoactivity    FAB1                    0
YER125W     RSP5                            2430 endocytosis ubiquitin-protein ligase activityG, plays RSP5 PM, endosome
                                                                                          M, P, N, cytoplasm?, Golgi,                     0
                      Ubiquitin-protein ligase involved in ubiquitin-mediated protein degradation;D, C a role in heat shock element (HSE)-mediated ge
YMR054W     STV1                            2673 vacuolar acidification two isoforms (Stv1p D Golgi
                                                                   one of                 V, G, and Vph1p); Stv1p is mechanism 7
                                                                                                                  STV1
                      Subunit of vacuolar-ATPase V0 domain, hydrogen-transporting ATPase activity, rotational located in V-ATPase complexes of the
YKL196C     YKT6                             603 (v-SNARE) with acyltransferase M, V, G, D, C          Golgi, MitoYKT6                    0
                      Vesicle membrane proteinvesicle fusion v-SNARE activity activity; involved in trafficking to and within the Golgi, endocytic traffic
YMR303C     ADH2                             alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of
                                                                                          C
                      Glucose-repressible1047 fermentation alcohol dehydrogenase activity cytoplasm ADH2                                  0
YAL038W     CDC19     Pyruvate kinase, functionsglycolysis
                                            1503 as a homotetramer in glycolysis to C
                                                                  pyruvate kinase activity             cytosol    CDC19                   0
                                                                                          convert phosphoenolpyruvate to pyruvate, the input for aerobic (TC
YCL040W     GLK1                            1503 glucose metabolism
                                                                  glucokinase activity C               cytosol     step of                0
                      Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible GLK1 glucose metabolism; one of three gluc
YBR011C     IPP1                             864 phosphate metabolism diphosphatase activitycytosol
                                                                  (PPase),                C                       IPP1                    0
                      Cytoplasmic inorganic pyrophosphatase inorganic catalyzes the rapid exchange of oxygens from Pi with water, highly expressed
YIR034C     LYS1                            1122 lysine biosynthesis via aminoadipic acid
                                                                  saccharopine dehydrogenase (NAD+, L-lysine-forming) activity 0
                                                                                          C            cytoplasm LYS1
                      Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final s
YOL126C     MDH2                            dehydrogenase, oneL-malate dehydrogenase activity
                                                                    of                    C            cytoplasm MDH2                     0
                      Cytoplasmic malate 1272 gluconeogenesis the three isozymes that catalyze interconversion of malate and oxaloacetate; involve
YLR303W     MET17                           1335 methionine metabolism synthase activity
                                                                  cysteine                C            cytoplasm MET17
                      O-acetyl homoserine-O-acetyl serine sulfhydrylase, required for sulfur amino acid synthesis                         0
YER091C     MET6                            2304 methionine biosynthesis
                                                                  5-methyltetrahydropteroyltriglutamate-homocysteine minimum of two glutamates
                                                                                          C            cytoplasm requires                 0
                      Cobalamin-independent methionine synthase, involved in amino acid biosynthesis; MET6 a S-methyltransferase activity on the
YFL045C     SEC53                            765 protein targeting to of
                                                                  phosphomannomutase activity cytosol
                                                                                          C                       SEC53                   0
                      Phosphomannomutase, involved in synthesis ERGDP-mannose and dolichol-phosphate-mannose; required for folding and glycos
YPL106C     SSE1                            2082 protein folding unfolded protein binding C             complex; SSE1                     0
                      ATPase that is a component of the heat shock protein Hsp90 chaperonecytoplasm binds unfolded proteins; member of the heat s
YPR074C     TKL1                            2043 pentose-phosphate shunt activity C
                                                                  transketolase                        cytoplasm TKL1
                      Transketolase, similar to Tkl2p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to 0           sedoheptulose-7-phosp
YKL035W     UGP1                            1500 protein (UGPase), glycosylation
                                                                  UTP-glucose0-phosphate uridylyltransferase activity
                                                                                          C            cytoplasm UGP1
                      UDP-glucose pyrophosphorylase amino acid catalyses the reversible formation of UDP-Glc from glucose 0               1-phosphate and UTP,
YMR116C     ASC1                            1233 unknown          unknown
                      WD repeat protein (G-beta like protein) involved in translation C, R              required for repression of Gcn4p activity in the absence
                                                                                          regulation;cytoplasm ASC1                       0
YLR075W     RPL10     Protein component of666 large (60S) ribosomal subunit, responsible for joining the RPL10 60S subunits; regulates translation i
                                               the protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R         cytosol     40S and                0
YNL069C   RPL16B                         1046 protein biosynthesis
                                                               structural constituent C, ribosome
                                                                                      of R       cytosol     RPL16B
                    N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has0similarity to Rpl16Ap, E
YMR242C   RPL20A    Protein component of973 large (60S) ribosomal subunit, nearly identical cytosol
                                            the protein biosynthesis
                                                               structural constituent C, ribosome Rpl20Bp and has similarity to 0 L18a ribosomal prot
                                                                                      of R       to          RPL20A                 rat
YOR063W   RPL3      Protein component of the large (60S) ribosomal subunit, has similarity tocytosol L3 and rat L3 ribosomal proteins; involved in the
                                         1164 protein biosynthesis
                                                               structural constituent C, ribosome coli
                                                                                      of R        E.         RPL3                   0
YDR012W   RPL4B                          1089 protein biosynthesis
                                                               structural constituent C, ribosome Rpl4Ap RPL4B
                                                                                      of R       to                                 0
                    Protein component of the large (60S) ribosomal subunit, nearly identical cytoplasm and has similarity to E. coli L4 and rat L4 ribo
YPL131W   RPL5      Protein component of894 large (60S) ribosomal subunit with similarity to cytosol L18RPL5rat L5 ribosomal 0
                                            the protein biosynthesis
                                                               structural constituent C, ribosome coli
                                                                                      of R        E.          and                   proteins; binds 5S rRNA
YGL076C   RPL7A     Protein component of the large (60S) ribosomal subunit, nearly identical cytosol
                                         1662 protein biosynthesis
                                                               structural constituent C, ribosome Rpl7Bp and has similarity to E. coli L30 and rat L7 rib
                                                                                      of R       to          RPL7A                  0
YGL147C   RPL9A     Protein component of576 large (60S) ribosomal subunit, nearly identical cytosol
                                            the protein biosynthesis
                                                               structural constituent C, ribosome Rpl9Bp and has similarity to E. coli L6 and rat L9 ribo
                                                                                      of R       to          RPL9A                  0
YLR340W   RPP0      Conserved ribosomal939 translational elongation human P0, and E. coli cytosolshown to be phosphorylated on serine 302
                                           protein P0 similar to rat P0, constituent C, ribosome
                                                               structural             of R        L10e;      RPP0                   0
YCR031C   RPS14A    Ribosomal protein 59721 protein biosynthesis
                                           of the small subunit, required for ribosome ribosome and 20S pre-rRNA processing; mutations confer cryp
                                                               structural constituent C, R
                                                                                      of assemblycytosol     RPS14A                 0
YML063W   RPS1B     Ribosomal protein 10768 protein biosynthesis subunit; nearly C, ribosomeRps1Ap and has similarity to rat S3a ribosomal protein
                                           (rp10) of the small structural constituent of R
                                                                (40S)                            cytosol
                                                                                      identical to           RPS1B                  0
YNL178W   RPS3      Protein component of723 small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viabi
                                            the protein biosynthesis
                                                               structural constituent C, ribosome
                                                                                      of R       cytosol     RPS3                   0
YJR145C   RPS4A                          1042 protein biosynthesis
                                                               structural constituent C, ribosome
                                                                                      of R       cytoplasm RPS4A                    0
                    Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has
YPL090C   RPS6A     Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to rat S6 ribosomal protein
                                         1105 protein biosynthesis
                                                               structural constituent C, ribosome
                                                                                      of R       cytosol     RPS6A                  0
YOR096W   RPS7A     Protein component of974 small (40S) ribosomal subunit, nearly identical cytosol
                                            the protein biosynthesis
                                                               structural constituent C, ribosome Rps7Bp; interacts with Kti11p; deletion causes hyper
                                                                                      of R        to         RPS7A                  0
YML028W   TSA1                            591 thioredoxin oxidative stress
                                                               thioredoxin peroxidase activity cytoplasm TSA1
                                                                                      C, R                                          1
                    Ubiquitous housekeeping response toperoxidase, reduces reactive oxygen, nitrogen and sulfur species using thioredoxin as hydr
YBL017C   PEP1      Type I transmembrane sorting receptor for to vacuole
                                         4740 protein targeting multiple vacuolar hydrolases; Golgi, pre-vacuole late-Golgi and prevacuolar endosom
                                                               signal sequence bindingG                      PEP1
                                                                                                  cycles between the                1
YDL192W   ARF1      ADP-ribosylation factor, GTPaseGolgi transport activityinvolved C regulation of coated formation vesicles in intracellular traffick
                                          546 ER to of the Ras superfamily
                                                               GTPase                 G, in      cytosol     ARF1                   0
YIL041W   GVP36                           981 unknown          unknown                G, C         Golgi vesicles; may              0
                    Golgi vesicle protein of unknown function; localizes to both early and latecytoplasm GVP36 interact with ribosomes, based on c
YNL044W   YIP3      Protein localized to COPII ER to Golgi transport be involved inG, E to Golgi transport; interacts with members of the Rab GTPase
                                          610 vesicles, proposed to
                                                               unknown                 ER                    YIP3
                                                                                                 COPII-coated vesicle               2
YGR175C   ERG1      Squalene epoxidase, catalyzes the epoxidation of squalene to L, E activity
                                         1491 ergosterol biosynthesis monooxygenase
                                                               squalene                          ER          ERG1                   in
                                                                                       2,3-oxidosqualene; plays an essential role 2 the ergosterol-biosyn
YMR152W   YIM1                           1098 response to DNA damage stimulus DNA damaging agents; the
                                                                displays                                                            0
                    Protein of unknown function; null mutant unknown sensitivity toL, M, C cytoplasm YIM1 authentic, non-tagged protein is detec
YIL124W   AYR1                            894 dihydroxyacetone phosphate reductase reductase activity
                                                               acylglycerone-phosphate found cytoplasm AYR1 ER, and mitochondrial outer membrane
                    NADPH-dependent 1-acylphosphatidic acid biosynthesis              L, M, E, C in lipid particles,                0
YBR041W   FAT1                           2010 very long-chain fatty acyl-CoA synthetase, mayactivity complex with Faa1p or Faa4p that imports and
                                                                                      L, P, X
                    Fatty acid transporter and lipid transport long-chain-fatty-acid-CoA ligasePM   form a FAT1                     3
YBL030C   PET9      Major ADP/ATP carrier of aerobic respiration
                                          957 the mitochondrial inner membrane,activity
                                                               ATP:ADP antiporter exchangesMito
                                                                                      M                      PET9                   3
                                                                                                   cytosolic ADP for mitochondrially synthesized ATP; req
YBR085W   AAC3      Mitochondrial inner membrane ADP/ATPATP:ADP antiporter activity
                                          924 anaerobic respiration
                                                                 translocator, exchanges cytosolic ADP forAAC3
                                                                                      M          Mito                               4
                                                                                                               mitochondrially synthesized ATP; expressed
YBR221C   PDB1      E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which isMitoevolutionarily-conserved multi-protein complex found
                                         1101 pyruvate metabolism dehydrogenase (acetyl-transferring) activity
                                                               pyruvate               M            an        PDB1                   0
YDR234W   LYS4      Homoaconitase, catalyzeslysine biosynthesis homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
                                         2082 the conversion of via aminoadipic acid activity
                                                               homoaconitate hydrataseM          Mito        LYS4                   0
YDR258C   HSP78     Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance 0
                                         2436 response to stress
                                                               ATPase activity        M          Mito        HSP78                  after heat shock; preven
YHR042W   NCP1      NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associatedNCP1
                                         2076 ergosterol biosynthesis transporter activity
                                                               electron               M          Mito                               0
                                                                                                              and coordinately regulated with Erg11p
YHR051W   COX6                            447 aerobic respiration
                                                               cytochrome-c oxidase activity mitochondrial inner membrane electron transport chain
                                                                                      M
                    Subunit VI of cytochrome c oxidase, which is the terminal member of theMito              COX6                   0
YIL125W   KGD1                           3045 tricarboxylic acid cycle
                                                               oxoglutarate dehydrogenase (succinyl-transferring) activity
                                                                                      M          Mito        KGD1
                    Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step0in the tricarboxylic acid
YJR016C   ILV3                           1758 branched chain family amino acid biosynthesis leading to ILV3
                                                               dihydroxy-acid dehydratase activity
                                                                                      M
                    Dihydroxyacid dehydratase, catalyzes third step in the common pathway Mito                                      0
                                                                                                              biosynthesis of branched-chain amino acids
YJR045C   SSC1                            ATPase that is a subunit of activity        M          Mito        SSC1                   0
                    Mitochondrial matrix1965 protein folding ATPasethe presequence translocase-associated protein import motor (PAM); involved in
YKL148C   SDH1      Flavoprotein subunit1923 tricarboxylic acid cycle (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the
                                          of succinate dehydrogenase dehydrogenase (ubiquinone) activity
                                                               succinate              M          Mito        SDH1                   0
YKL150W   MCR1                            909 response reductase, stress in ergosterol biosynthesis MCR1
                                                               cytochrome-b5          M
                    Mitochondrial NADH-cytochrome b5to oxidative involved reductase activity     Mito                               1
YLR259C   HSP60                          1719 protein folding single-stranded DNA M   binding Mito           HSP60                  0
                    Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly;
YLR355C   ILV5                           1188 mitochondrial genome maintenance M
                                                               ketol-acid protein involved in branched-chain amino acid biosynthesis, also required for
                    Acetohydroxyacid reductoisomerase, mitochondrialreductoisomerase activity    Mito        ILV5                   0
YMR108W   ILV2                           2064 branched chain common step acid biosynthesis valine
                                                                  family amino in isoleucine and
                                                                                      M                      ILV2                   0
                    Acetolactate synthase, catalyses the firstacetolactate synthase activity Mito biosynthesis and is the target of several classes
YMR203W   TOM40     Component of the TOM (translocase of protein import
                                         1164 mitochondral outer membrane) complex responsible for recognition and initial import steps for all mitoc
                                                               protein transporter activity
                                                                                      M          Mito        TOM40                  0
YMR241W   YHM2                            945 mitochondrial genome the mitochondrial nucleoid structure, involved in mtDNA replication and segreg
                                                               DNA binding
                    Mitochondrial DNA-binding protein, component ofmaintenance M                 Mito        YHM2                   0
YNL055C   POR1      Mitochondrial porin (voltage-dependent anion channel),ion-selective channel activity
                                          852 aerobic respiration
                                                               voltage-gated outer membraneMito
                                                                                      M                      POR1                   0
                                                                                                  protein required for the maintenance of mitochondrial os
YOR065W   CYT1      Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulatedCoQH2-cytochrome c reductase comp
                                          930 mitochondrial electron transport, ubiquinol to cytochrome CYT1 by the heme-activated, glucose-repres
                                                               electron transporter, transferring electrons within
                                                                                      M          Mito         c                     0
YBL015W   ACH1                           1581 acetate metabolismto mitochondria; required
                                                                                       activity Mito         ACH1                   0
                    Acetyl-coA hydrolase, primarily localized acetyl-CoA hydrolaseM, Cfor acetate utilization and for diploid pseudohyphal growth
YCR004C   YCP4                            744 unknown          electron transporter activity
                                                                                      M, C       cytoplasm YCP4                     0
                    Protein of unknown function, has sequence and structural similarity to flavodoxins; the authentic, non-tagged protein is detected
YDR141C   DOP1                           5097 cellular morphogenesis
                                                               unknown                M, C       cytoplasm DOP1                     0
                    Protein of unknown function, essential for viability, involved in establishing cellular polarity and morphogenesis; the authentic, no
YGR086C   PIL1                           of eisosomes, to heat large immobile patch structures at the cell
                                                                 are                  M, C                                          0
                    Primary component 1020 responsewhichprotein kinase inhibitor activity cytoplasm PIL1cortex associated with endocytosis, alon
YGR234W   YHB1                           1200 response to stress oxide reductase oxide
                                                               nitric                  activity cytoplasm YHB1                      0
                    Nitric oxide oxidoreductase, flavohemoglobin involved in nitric M, C detoxification; plays a role in the oxidative and nitrosative str
YHR183W   GND1                            dehydrogenase (decarboxylating),
                                                               phosphogluconate dehydrogenase (decarboxylating) activity
                                                                                      M, an      cytoplasm GND1
                    6-phosphogluconate1470 response to oxidative stress catalyzesC NADPH regenerating reaction in the0               pentose phosphate pat
YJR104C   SOD1                             dismutase; some mutations are analogous dismutase activitySOD1 (amyotrophic lateral sclerosis) in huma
                                                               copper,                M, C
                    Cytosolic superoxide 465 zinc ion homeostasis zinc superoxide to thosecytosol  that cause ALS                   0
YLR304C   ACO1                           for the tricarboxylic genome maintenance M, C
                                                               aconitate cycle and also independently required for mitochondrial genome maintenance
                    Aconitase, required 2337 mitochondrial acid (TCA)hydratase activity          cytosol     ACO1                   0
YMR186W   HSC82                          2118 the Hsp90 stress unfolded protein function
                                                                                      M,         cytoplasm HSC82                    0
                    Cytoplasmic chaperone ofresponse tofamily, redundant inbinding Cand nearly identical with Hsp82p, and together they are essen
YNL239W   LAP3                           1365 response to antibiotic
                                                               transcription regulator activity cytoplasm LAP3
                                                                                      M, C                                           in
                    Aminopeptidase of cysteine protease family, has a DNA binding activity and acts as bleomycin hydrolase0 vitro; transcription is
YPL004C   LSP1                           of eisosomes, to heat large immobile patch structures at the cell cortex associated with endocytosis, alon
                                                                 are                  M, C
                    Primary component 1026 responsewhichprotein kinase inhibitor activity cytoplasm LSP1                            0
YPL061W   ALD6                           1503 acetate biosynthesis
                                                               aldehyde dehydrogenase NADP+
                                                                                      M, C       cytoplasm ALD6                     0
                    Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizesactivity as the preferred coenzyme; required for conversion o
YDR450W   RPS18A                            the protein biosynthesis
                                                               structural constituent M, ribosome Rps18Bp and has similarity to0E. coli S13 and rat S18
                                                                                      of C, R to
                    Protein component of876 small (40S) ribosomal subunit; nearly identical Mito             RPS18A
YEL047C   YEL047C                        1413 metabolism fumarate reductase for C, R Mito
                                                                                      M, anaerobic growth;YEL047C
                    Soluble fumarate reductase, required with isoenzyme Osm1p(NADH) activity                                        0
                                                                                                              may interact with ribosomes, based on co-pu
YER074W   RPS24A                            the protein biosynthesis
                                                               structural constituent M, ribosome
                                                                                      of to Rps24Bp          RPS24A                 0
                    Protein component of874 small (40S) ribosomal subunit; identicalC, R Mito and has similarity to rat S24 ribosomal protein
YER004W   FMP52                           696 unknown
                    Protein of unknown function, localized to unknown
                                                                the mitochondrial outer membrane
                                                                                      M, E       Mito        FMP52                  0
YML128C   MSC1                           1542 meiotic recombination
                                                               unknown                M, E       Mito        MSC1
                    Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous0chromatids; the authen
YEL031W     SPF1      P-type ATPase, ion 3648 protein amino acid glycosylation
                                            transporter of the ER membrane involved M, ER function and Ca2+ homeostasis; required for regulating Hmg
                                                                   ATPase activity, coupledE transmembrane movement of ions,10
                                                                                             in to       Mito        SPF1                    phosphorylative mechan
YAR002C-A   ERP1                               660 ER to Golgi transport
                                                                   unknown                   M, and Mito             ERP1                    2
                      Protein that forms a heterotrimeric complex with Erp2p, Emp24p, G Erv25p; member, along with Emp24p and Erv25p, of the p
YER031C     YPT31     GTPase of the Ypt/Rab family, very similar to Ypt32p; involved in G, Dexocytic pathway; mediates intra-Golgi traffic or the buddin
                                               672 vesicle-mediated transport
                                                                   GTPase activity           M, the      Golgi       YPT31                   0
YGL210W     YPT32     GTPase of the Ypt/Rab family, very similar to Ypt31p; involved in G, Dexocytic pathway; mediates intra-Golgi traffic or the buddin
                                               669 vesicle-mediated transport
                                                                   GTPase activity           M, the      Golgi       YPT32                   0
YKL212W     SAC1                            1872 exocytosis        inositol or Golgi, involved inE Mitotrafficking and secretion
                                                                                             M, G, protein
                      Lipid phosphoinositide phosphatase of the ER and phosphatidylinositol phosphatase activity     SAC1                    2
YDR155C     CPR1                               489 sporulation     peptidyl-prolyl cis-trans isomerase activity isomerization of peptide bonds N-terminal to
                                                                                             M, N        nucleus
                      Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-transCPR1                             0
YNL135C     FPR1                               345 ribosome assembly
                                                                   peptidyl-prolyl cis-trans isomerase rapamycin; also
                                                                                             M, N, C cytoplasm                               0
                      Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and activity FPR1 binds to the nonhistone chromatin bi
YGL253W     HXK2                            1461 replicative cell hexokinase activity M, N, C nucleus HXK2
                                                                   aging                                                                     0
                      Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on gluc
YDR046C     BAP3                            1815 amino the uptake of cysteine, leucine, P
                                                                   amino                     M, isoleucine
                      Amino acid permease involved inacid transport acid transporter activity Mitoand valine         BAP3                   12
YLR342W     FKS1      Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory
                                            5631 cell wall organization and biogenesis Pactivity
                                                                   1,3-beta-glucan synthase  M,                      FKS1
                                                                                                         actin cortical patch               13
YDR343C     HXT6      High-affinity glucose1713 hexose transport facilitator superfamily, nearly identical HXT6
                                              transporter of the major
                                                                   glucose transporter activity
                                                                                             M, P        Mito                               at
                                                                                                                      to Hxt7p, expressed 11 high basal levels relat
YDR033W     MRH1                                primarily to the
                      Protein that localizes 963 unknown plasma membrane, also found at the nuclear envelope; has similarity to Hsp30p and Yro2p,
                                                                   unknown                   M, P        Mito        MRH1                    7
YOR153W     PDR5                            4536 response to drug  xenobiotic-transporting ATPase Mito
                                                                                             M, P        activity PDR5                      14
                      Short-lived membrane ABC (ATP-binding cassette) transporter, actively exports various drugs, expression regulated by Pdr1p; a
YDR011W     SNQ2                            4506 response to drug  xenobiotic-transporting ATPase Mito
                                                                                             M, P        activity SNQ2
                      ABC transporter protein involved in multidrug resistance and resistance to singlet oxygen species                     12
YOL109W     ZEO1      Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by
                                               342 cell wall organization and biogenesis P
                                                                   unknown                   M,          Mito        ZEO1                    0
YIL105C     SLM1                            2061 actin cytoskeleton organization and biogenesis acts downstream
                                                                   phosphoinositide binding Slm2p;
                                                                                             M,
                      Phosphoinositide PI4,5P(2) binding protein, forms a complex withP, C cytoplasm SLM1 of Mss4p in a0                       pathway regulating act
YHR135C     YCK1                            1617 protein amino casein kinase IIactivity shares redundantYCK1
                                                                    casein kinase isoform;
                      Palmitoylated, plasma membrane-boundacid phosphorylation M, P, E ER                                                     in
                                                                                                                       functions with Yck2p 0 morphogenesis, prop
YPR149W     NCE102                             522 protein secretion
                                                                   unknown                   M, P, E, Ccytoplasm NCE102                      4
                      Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory sig
YKL081W     TEF4      Translation elongation factor EF-1 gamma
                                            1565 translational elongation elongationM, R activity
                                                                   translation                factor     Mito        TEF4                    0
YLR001C     YLR001C                         2589 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YLR001C
                                                                    authentic,               M, V        Mito                                1
                                                                                                                     in highly purified mitochondria in high-through
YML001W     YPT7      GTPase; GTP-binding protein of the rab family; required for homotypic fusion eventYPT7
                                               627 vesicle-mediated transport
                                                                   GTPase activity           M, V        vacuole                             0
                                                                                                                       in vacuole inheritance, for endosome-endoso
YOR316C     COT1      Vacuolar transporter1320 mediateshomeostasis into inorganic cation transporter activity resistance to cobalt and rhodium
                                               that zinc ion zinc transport
                                                                   di-, tri-valent the vacuole; overexpression confers
                                                                                             M, V        Mito, vacuole
                                                                                                                     COT1                    6
YPL154C     PEP4                            1218 sporulation       endopeptidase activity , VM           vacuole PEP4                        0
                      Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; synthe
YHR186C     KOG1                            4674 unknown           unknown                    growth Mito            KOG1                    0
                      Subunit of TORC1, a rapamycin-sensitive complex involved inM, V, P control that contains Tor1p or Tor2p, Lst8p and Tco89p; co
YKL203C     TOR2                            7425 and rapamycin target; subunit               M, V, a Mito, vacuole, PM
                      PIK-related protein kinaseendocytosis protein binding of TORC1,P complex thatTOR2                                      0
                                                                                                                       regulates growth in response to nutrients and
YLL024C     SSA2                             involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family;
                      ATP binding protein 1920 protein folding ATP binding                   M, V, W, C  cytoplasm SSA2                      0
YCR005C     CIT2      Citrate synthase, catalyzes the condensation of(Si)-synthase activity
                                            1383 glutamate biosynthesis
                                                                   citrate acetyl coenzyme X and oxaloacetate to form citrate, peroxisomal isozyme involv
                                                                                             M, A        Mito        CIT2                    0
YJR019C     TES1                            1050 fatty acid likelyacyl-CoA thioesterase activity Mito
                                                                    to be                    M, X                    TES1                    0
                      Peroxisomal acyl-CoA thioesterase oxidation involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein
YKR001C     VPS1                               vacuolar protein sorting, functions
                                                                   GTPase activity           M, X, C cytoplasm VPS1                          0
                      GTPase required for2115 protein targeting to vacuole in actin cytoskeleton organization via its interaction with Sla1p; required f
YNL009W     IDP3                            1263 fatty isocitrate isocitrate
                                                                    dehydrogenase, catalyzes C cytoplasm
                                                                                             M, X, oxidation of isocitrate                   0
                      Peroxisomal NADP-dependent acid beta-oxidation dehydrogenase (NADP+) activityIDP3 to alpha-ketoglutarate with the formatio
YFL039C     ACT1                            1436 cell wall cell polarization, endocytosis, and other cytoskeletal functions
                                                                   structural constituent N              actin
                      Actin, structural protein involved inorganization and biogenesisof cytoskeleton cortical patch ACT1                    0
YER177W     BMH1                                isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle
                      14-3-3 protein, major 804 sporulation        protein binding           N           nucleus BMH1                        0
YDR099W     BMH2                                isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle
                      14-3-3 protein, minor 822 sporulation        protein binding           N           nucleus BMH2                        0
YDR190C     RVB1                            1392 in transcriptionATPase activity
                                                                    regulation; component     polymerase II remodeling complexes; required for assembly and
                                                                                                         nucleus RVB1
                      Essential protein involved regulation of transcription from RNAN of chromatinpromoter                                  0
YLR044C     PDC1                            1692 pyruvate metabolism decarboxylase C
                                                                   pyruvate                  in activity cytoplasm PDC1                      0
                      Major of three pyruvate decarboxylase isozymes, key enzyme N, alcoholic fermentation, decarboxylates pyruvate to acetaldehyde
YDL124W     YDL124W                            939 metabolism alpha-keto amide reductase activity
                                                                                             N, C        cytoplasm YDL124W                   0
                      NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic
YNL134C     YNL134C                         1131 unknown             similarity to dehydrogenases from other YNL134C
                                                                                             N, C        cytoplasm                           0
                      Putative protein of unknown function withalcohol dehydrogenase (NADP+) activity model organisms; green fluorescent protein (G
YPL265W     DIP5                            1827 amino acid transport acid transporter
                                                                   amino                     high-capacity           DIP5                   11
                      Dicarboxylic amino acid permease, mediates high-affinity and P activity PM transport of L-glutamate and L-aspartate; also a tran
YNL098C     RAS2                               969 sporulation     GTPase activity           P           PM          RAS2
                      GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth;0farnesylation and palm
YLR229C     CDC42     Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of
                                               576 establishment of cell polarity
                                                                   GTPase activity           P, C        PM          CDC42                   0
YJL138C     TIF2                              factor eIF4A, identical to Tif1p; initiation factor activity
                                                                   translation DEA(D/H)-box RNA cytoplasm TIF2
                      Translation initiation1188 translational initiation                    R, C                                            0
                                                                                                         helicase that couples ATPase activity to RNA binding an
YBL050W     SEC17                              995 ER required for vesicular transport between ERactivity
                                                                   soluble                   U             and Golgi and for                 0
                      Peripheral membrane protein to Golgi transport NSF attachment proteinmembraneSEC17 the 'priming' step in homotypic vacuo
YMR266W     RSN1                            2862 unknown           unknown                   U           unknown RSN1
                      Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant                       11
YLR447C     VMA6                            1038 vacuolar acidification
                                                                   hydrogen-transporting     vacuolar activity, rotational
                                                                                                         vacuole
                      Subunit d of the five-subunit V0 integral membrane domain of VATPaseH+-ATPaseVMA6 mechanism 0   (V-ATPase), an electrogenic proton pump fou
YPR026W     ATH1                            3636 response to stressalpha,alpha-trehalase activity cell wall ATH1
                      Acid trehalase required for utilization of extracellular trehalose V, W                                                1
YGR189C     CRH1                            1524 unknown             have a role in cell wall architecture wall CRH1
                      Putative glycosidase of the cell wall, mayunknown                      W           cell                                0
YMR215W     GAS3                            1575 unknown            has similarity to Gas1p; localizes towall GAS3
                                                                                             W           cell the
                      Putative 1,3-beta-glucanosyltransferase, 1,3-beta-glucanosyltransferase activity cell wall                             0
YOL030W     GAS5                            1455 unknown            has similarity to Gas1p; localizes towall GAS5
                                                                                             W           cell the
                      Putative 1,3-beta-glucanosyltransferase, 1,3-beta-glucanosyltransferase activity cell wall                             0
YJL159W     HSP150                          1164 cell wall organization and biogenesisof attached to the cell wall
                                                                   is secreted and covalently                                                0
                      O-mannosylated heat shock protein that structural constituent W cell wallcell wall HSP150 via beta-1,3-glucan and disulfide brid
YMR008C     PLB1                            1995 glycerophospholipid in lipid metabolism,
                                                                   lysophospholipase W                   cell wall PLB1                      0
                      Phospholipase B (lysophospholipase) involvedmetabolism activity required for deacylation of phosphatidylcholine and phosphati
YBR222C     PCS60                           1632 protein,          AMP both the              X, C        cytoplasm PCS60                     0
                      Peroxisomal AMP-bindingunknownlocalizes to binding peroxisomal peripheral membrane and matrix, expression is highly induci
YLR027C     AAT2                            1257 nitrogen compound metabolism
                                                                   aspartate transaminase C  X, activity cytoplasm peroxisomes in oleate-grown cells
                      Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to AAT2                               0
YMR221C     FMP42                           1515 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP42 purified mitochondria in high-through
                                                                    authentic,               M, V        Mito        in highly              11
YDL226C     GCS1                            1059 ER to activating protein
                                                                   actin binding             G, D, C cytoskeleton    GCS1                    0
                      ADP-ribosylation factor GTPase Golgi transport (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gl
YNL297C     MON2                            4911 endocytosis in endocytosis and vacuoleCfactor activity MON2
                                                                                             G, D,       endosome, cytosol                   0
                      Peripheral membrane protein with a role guanyl-nucleotide exchangeintegrity, interacts with Arl1p and localizes to the endosome
YIL048W     NEO1                            3456 retrograde transport, Golgi to ER G, D ATPase activityNEO1
                                                                   phospholipid-translocating            ER                                  5
                      Protein involved in the retrograde transport from the Golgi complex to the ER and the endosomal membrane traffic; putative amin
YLR148W     PEP3                            membrane protein that vacuole vesicular docking/fusion reactions in conjunction with SNARE proteins, req
                                                                   protein binding
                      Vacuolar peripheral 2757 Endosome late topromotestransport V, D                    vacuole PEP3                        0
YMR231W     PEP5                            membrane protein required for protein trafficking
                                                                   unknown                                vacuole PEP5                       0
                      Peripheral vacuolar 3090 Endosome late to vacuole transport V, D andvacuole biogenesis; forms complex with Pep3p that pro
YJR066W     TOR1      PIK-related protein kinasemeiosis
                                            7413 and rapamycin target;binding of TORC1,N, complex thatTOR1
                                                                   protein subunit           V, P, a G, DPM                                  0
                                                                                                                       controls growth in response to nutrients by re
YDR080W   VPS41                           protein that is a subunit fusion, non-autophagic fusion and endosome
                                                               Rab guanyl-nucleotide D  vacuole vacuole, vacuole
                                                                                                              VPS41
                    Vacuolar membrane2979 homotypic vacuole of the homotypic V,exchange factor activity protein sorting 0          (HOPS) complex; esse
YMR110C   YMR110C                        1599 unknown          unknown                 L, M, outer membrane and also in lipid particles; has similarity to h
                    Putative fatty aldehyde dehydrogenase, located in the mitochondrialD Mito                 YMR110C              1
YKR014C   YPT52                            705 endocytosis GTPase activity             D           for vacuolar protein
                    GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; requiredendosomeYPT52 sorting and 0                endocytosis
YMR120C   ADE17     Enzyme of 'de novo'1779 de novo' IMP biosynthesisboth 5-aminoimidazole-4-carboxamide ribonucleotide0
                                          purine biosynthesis IMP cyclohydrolase activity
                                                               containing              C          cytosol     ADE17                 transformylase and ino
YBR288C   APM3      Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in endosome? alkaline phosphatase to the vacuole v
                                         1452 vesicle-mediated transport
                                                               unknown                 C                      APM3
                                                                                                  Golgi, transport of              0
YNL271C   BNI1                           5862 actin filament organization
                                                                actin filaments, involved in cell processes BNI1 as budding and mitotic spindle orientatio
                                                                                       C          bud neck such
                    Formin, nucleates the formation of linearcytoskeletal regulatory protein binding                               0
YPL111W   CAR1      Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repress
                                         1002 arginine catabolismion ornithine
                                                               zinc to binding         C          cytosol     CAR1                 0
YLL050C   COF1      Cofilin, promotes actin filament filament organization
                                           611 actin depolarization in binding
                                                               protein a pH-dependent manner; binds both actin monomers and filaments and severs
                                                                                       C          actin cortical patch
                                                                                                              COF1                 0
YJR109C   CPA2                           3357 arginine biosynthesis
                                                               carbamoyl-phosphateC      synthase carbamoyl-phosphate synthase complex
                                                                                                  (glutamine-hydrolyzing) activity 0
                                                                                                              CPA2
                    Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor
YAL012W   CYS3                           1185 sulfur amino one metabolism
                                                               cystathionine gaMMa-lyase activitythe transsulfuration pathway that yields cysteine from
                                                                                       C          in
                    Cystathionine gamma-lyase, catalyzes acid of the two reactions involved cytoplasm CYS3                         0
YOR204W   DED1                           1815 translational initiation
                                                               RNA RNA helicase, required for translation initiation of all yeast 0
                    ATP-dependent DEAD (Asp-Glu-Ala-Asp)-boxhelicase activityC                    cytoplasm DED1                   mRNAs; mutations in h
YLL018C   DPS1                           1674 protein biosynthesis binding
                                                               RNA                     that       cytoplasm DPS1                   0
                    Cytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme C catalyzes the specific aspartylation of tRNA(Asp); class II ami
YBL101C   ECM21                          3354 cell wall organization and biogenesis
                                                               unknown
                    Non-essential protein of unknown function; promoter contains C                cytoplasm ECM21
                                                                                       several Gcn4p binding elements              0
YJL167W   ERG20     Farnesyl pyrophosphate synthetase, biosynthesis
                                         1059 ergosterol hasdimethylallyltranstransferase activity geranyltranstransferase activities; catalyzes the
                                                                both dimethylallyltranstransferase and
                                                                                       C          cytosol     ERG20                0
YPR035W   GLN1                           1113 nitrogen compound metabolism
                                                                glutamine from glutamate activity
                                                                                       C          cytoplasm GLN1                   0
                    Glutamine synthetase (GS), synthesizes glutamate-aMMonia ligase and ammonia; with Glt1p, forms the secondary pathway for g
YDR171W   HSP42                          1128 response to stress
                                                               unfolded protein bindingC          cytoplasm HSP42                  0
                    Small cytosolic stress-induced chaperone that forms barrel-shaped oligomers and suppresses the aggregation of non-native prot
YPL240C   HSP82                          2130 response to required for pheromone signalingcytoplasm HSP82
                    Cytoplasmic chaperone (Hsp90 family)stress ATPase activity, coupledC                                           0
                                                                                                   and negative regulation of Hsf1p; docks with the mitoch
YFR053C   HXK1                           1458 a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is
                    Hexokinase isoenzyme 1, glucose metabolism hexokinase activity C              cytosol     HXK1                 0
YLR174W   IDP2                           1239 glutamate biosynthesis dehydrogenase (NADP+) activity to alpha-ketoglutarate; levels are elevated du
                                                               isocitrate              C          cytosol
                    Cytosolic NADP-specific isocitrate dehydrogenase, catalyzes oxidation of isocitrate IDP2                       0
YLR432W   IMD3                           1572 GTP biosynthesis  catalyzes the first step of GMP cytoplasm IMD3
                    Inosine monophosphate dehydrogenase,IMP dehydrogenase activity     C                                           0
                                                                                                   biosynthesis, member of a four-gene family in S. cerevi
YMR004W   MVP1                           1536 protein targeting to vacuole
                                                               unknown                 C          cytoplasm MVP1                   0
                    Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp
YHR001W   OSH7                           1314 steroid biosynthesis
                                                               oxysterol seven         C           cerevisiae; family              0
                    Member of an oxysterol-binding protein family withbindingmembers in S. unknown OSH7 members have overlapping, redundan
YDR071C   PAA1                             576 establishment and/or maintenance of chromatin activity PAA1
                                                               aralkylamine N-acetyltransferase architecture
                                                                                       C          cytoplasm                        0
                    Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptam
YHR063C   PAN5                           1140 pantothenate biosynthesis
                                                               2-dehydropantoate pathway, structurally homologous to E. coli panE
                                                                                       C          activity
                    2-dehydropantoate 2-reductase, part of the pantothenic acid 2-reductase cytoplasm PAN5                         1
YKL127W   PGM1                           1713 glucose 1-phosphate the conversionactivity cytosol
                                                               phosphoglucomutaseCfrom glucose-1-phosphate to glucose-6-phosphate, which is a ke
                    Phosphoglucomutase, minor isoform; catalyzes utilization                                  PGM1                 0
YER023W   PRO3                             861 proline biosynthesis
                                                               pyrroline-5-carboxylate reductase biosynthesis
                                                                                       C          cytoplasm
                    Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in prolineactivity PRO3                     0
YDL135C   RDI1                             609 actin filament in the localization andC
                                                               signal transducer activity
                    Rho GDP dissociation inhibitor involved organization                           of Cdc42pRDI1
                                                                                        regulationcytosol                          0
YNL163C   RIA1                           3333 ribosomal large subunit activity
                                                               GTPase 60S ribosome;               cytoplasm RIA1                   0
                    Cytoplasmic GTPase involved in biogenesis of thebiogenesis C has similarity to translation elongation factor 2 (Eft1p and Eft2p
YER043C   SAH1                           1350 methionine metabolism
                                                               adenosylhomocysteinase activity
                                                                                       C          cytoplasm SAH1                   0
                    S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated m
YLR180W   SAM1                           1149 methionine metabolism adenosyltransferasecytoplasm to the
                                                               methionine              C           activity                        0
                    S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP SAM1 sulfur atom of methionine; one of two d
YOR184W   SER1                           1188 purine base biosynthesis
                                                               phosphoserine transaminase activity from SER1
                    3-phosphoserine aminotransferase, catalyzes the formation ofC                 cytoplasm 3-phosphohydroxypyruvate, required for serin
                                                                                        phosphoserine                              0
YMR165C   SMP2                           2589 aerobic respiration
                                                               unknown                 C          cytoplasm SMP2                   0
                    Mg2+-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and P<sub>i</s
YLL021W   SPA2                           4401 actin filament organization regulatory protein binding
                                                               cytoskeletal            C          bud neck SPA2
                    Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; 0     acts as a scaffold for M
YBL075C   SSA3      ATPase involved in protein folding andstress
                                         1950 response to the response to stress;C
                                                               ATPase activity                    cytosol     SSA3                 0
                                                                                        plays a role in SRP-dependent cotranslational protein-membrane
YLR250W   SSP120                           705 protein secretion
                                                               unknown                 C          cytoplasm SSP120                 0
                    Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YHR064C   SSZ1                           1617 protein biosynthesis
                                                               unfolded protein bindingC          cytoplasm SSZ1                   0
                    Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via th
YOR027W   STI1                           1770 protein folding unfolded of the cytosolicC           chaperones; activates the ATPase activity of Ssa1p; ho
                    Hsp90 cochaperone, interacts with the Ssa group protein binding Hsp70 cytoplasm STI1                           0
YDR212W   TCP1                           1680 protein folding unfolded protein mediatesC          cytoplasm TCP1                   0
                    Alpha subunit of chaperonin-containing T-complex, which binding protein folding in the cytosol; involved in maintenance of actin
YDL091C   UBX3                           1368 unknown          unknown                 C           Cdc48p, UBX3                    1
                    UBX (ubiquitin regulatory X) domain-containing protein that interacts withcytoplasmgreen fluorescent protein (GFP)-fusion protein
YKL216W   URA1                             945 'de novo' pyrimidine base enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydr
                                                               dihydroorotate dehydrogenase activity
                    Dihydroorotate dehydrogenase, catalyzes the fourth biosynthesis    C          cytoplasm URA1                   0
YPL120W   VPS30                          membrane-associated complex with Apg14p that is essential for autophagy; involved0in a retrieval step of th
                                                               unknown
                    Protein that forms a 1674 protein targeting to vacuole             C          membraneVPS30
YNL197C   WHI3                            that regulation CLN3 mRNA in cytoplasmic foci; cytoplasmic retention factor for Cdc28p and associated cy
                                                               RNA binding
                    RNA binding protein1986 sequesters of cell size                    C          cytoplasm WHI3                   0
YBR025C   YBR025C                        1185 unknown          unknown
                    Putative protein of unknown function; identified as specifically C             with the proteasome;            0
                                                                                       interactingcytoplasm YBR025C protein levels are induced by hydr
YNL064C   YDJ1                           1230 ER-associated of the HSP90 and HSP70 functions; involved in protein translocation across membranes
                                                                protein catabolism C
                    Protein chaperone involved in regulation chaperone regulator activity         cytosol     YDJ1                 0
YFR006W   YFR006W                        1608 unknown          X-Pro aminopeptidase activity cytoplasm YFR006W
                                                                                       C                                           1
                    Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an es
YGR130C   YGR130C                        2451 unknown          unknown                 C          cytoplasm YGR130C                0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; specifically phos
YKL047W   YKL047W                        1551 unknown          unknown                 C          cytoplasm YKL047W
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm0
YLL029W   YLL029W                        2250 unknown          unknown                 C          cytoplasm YLL029W                0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not
YMR124W   YMR124W                        2832 unknown          unknown                 C          cytoplasm YMR124W                0
                    Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and b
YNL040W   YNL040W                        1371 unknown            strong similarity to alanyl-tRNAcytoplasm YNL040W
                    Putative protein of unknown function withunknown                   C                                           0
                                                                                                   synthases from Eubacteria; green fluorescent protein (G
YPR102C   RPL11A    Protein component of525 large (60S) ribosomal subunit, nearly identical cytosol
                                            the protein biosynthesis
                                                               structural constituent C, ribosome Rpl11Bp; involved in ribosomal assembly; depletion c
                                                                                        of R      to          RPL11A               0
YEL054C   RPL12A    Protein component of498 large (60S) ribosomal subunit, nearly identical cytosol
                                            the protein biosynthesis
                                                               structural constituent C, ribosome Rpl12Bp; rpl12a rpl12b double mutant exhibits slow g
                                                                                        of R      to          RPL12A               0
YMR142C   RPL13B    Protein component of the large (60S) ribosomal subunit, nearly identical cytosol
                                         1002 protein biosynthesis
                                                               structural constituent C, ribosome Rpl13Ap; not essential for viability; has similarity to ra
                                                                                        of R      to          RPL13B               0
YKL006W   RPL14A    N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Bp and has similarity to
                                           815 protein biosynthesis
                                                               structural constituent C, ribosome
                                                                                        of R      cytosol     RPL14A               0
YHL001W   RPL14B    Protein component of815 large (60S) ribosomal subunit, nearly identical cytosol
                                            the protein biosynthesis
                                                               structural constituent C, ribosome Rpl14Ap and has similarity to 0 L14 ribosomal prote
                                                                                        of R      to          RPL14B               rat
YIL133C   RPL16A                           890 protein biosynthesis
                                                               structural constituent C, ribosome
                                                                                        of R      cytosol     RPL16A
                    N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has0similarity to Rpl16Bp, E
YKL180W   RPL17A                            the protein biosynthesis
                                                               structural constituent C, ribosome Rpl17Bp and has
                                                                                        of R      to                               E.
                    Protein component of861 large (60S) ribosomal subunit, nearly identical cytoplasm RPL17A similarity to 0 coli L22 and rat L17
YJL177W   RPL17B    Protein component of872 large (60S) ribosomal subunit, nearly identical cytosol
                                            the protein biosynthesis
                                                               structural constituent C, ribosome Rpl17Ap and has similarity to 0 coli L22 and rat L17
                                                                                        of R      to          RPL17B               E.
YOL120C   RPL18A                         1008 protein biosynthesis
                                                               structural constituent C, R Rpl18Bp and has similarity to rat L180ribosomal protein; intro
                                                                                        of to
                    Protein component of the large (60S) ribosomal subunit, identicalribosome     cytosol     RPL18A
YNL301C     RPL18B                            the protein biosynthesis
                                                                  structural constituent C, R Rpl18Ap and has similarity to rat L180ribosomal protein
                                                                                          of to
                      Protein component of993 large (60S) ribosomal subunit, identicalribosome       cytosol    RPL18B
YBR084C-A   RPL19A    Protein component of the large (60S) ribosomal subunit, nearly identical cytosol
                                            1076 protein biosynthesis
                                                                  structural constituent C, ribosome Rpl19Bp and has similarity to 0 L19 ribosomal prote
                                                                                          of R       to         RPL19A                 rat
YBL027W     RPL19B    Protein component of954 large (60S) ribosomal subunit, nearly identical cytosol
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome Rpl19Ap and has similarity to 0 L19 ribosomal prote
                                                                                          of R       to         RPL19B                 rat
YBL087C     RPL23A    Protein component of918 large (60S) ribosomal subunit, identicalribosome
                                              the protein biosynthesis
                                                                  structural constituent C, R Rpl23Bp and has similarity to E. coli 0 and rat L23 ribosom
                                                                                          of to      cytosol    RPL23A                 L14
YER117W     RPL23B    Protein component of885 large (60S) ribosomal subunit, identicalribosome
                                              the protein biosynthesis
                                                                  structural constituent C, R Rpl23Ap and has similarity to E. coli 0 and rat L23 ribosom
                                                                                          of to      cytosol    RPL23B                 L14
YGL031C     RPL24A    Ribosomal protein L30 of the large (60S)structural constituent C, ribosome to Rpl24Bp and has similarity to rat L24 ribosomal pr
                                             468 protein biosynthesis
                                                                   ribosomal subunit, nearly identical
                                                                                          of R       cytosol    RPL24A                 0
YGR148C     RPL24B    Ribosomal protein L30 of the large (60S)structural constituent C, ribosome to Rpl24Ap and has similarity to rat L24 ribosomal pr
                                             468 protein biosynthesis
                                                                   ribosomal subunit, nearly identical
                                                                                          of R       cytosol    RPL24B                 0
YDR471W     RPL27B    Protein component of795 large (60S) ribosomal subunit, nearly identical cytosol
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome Rpl27Ap and has similarity to 0 L27 ribosomal prote
                                                                                          of R       to         RPL27B                 rat
YFR031C-A   RPL2A     Protein component of912 large (60S) ribosomal subunit, identicalribosome and has similarity to E. coli L2 and rat L8 ribosomal
                                              the protein biosynthesis
                                                                  structural constituent C, R Rpl2Bp
                                                                                          of to      cytosol    RPL2A                  0
YBL092W     RPL32                             the protein biosynthesis
                                                                  structural constituent C, ribosome L32 RPL32
                                                                                          of R        rat                              0
                      Protein component of393 large (60S) ribosomal subunit, has similarity tocytosol ribosomal protein; overexpression disrupts telom
YDL136W     RPL35B                            the protein biosynthesis
                                                                  structural constituent C, R Rpl35Ap and has similarity to rat L350ribosomal protein
                                                                                          of to
                      Protein component of768 large (60S) ribosomal subunit, identicalribosome       cytosol    RPL35B
YLR325C     RPL38                             the protein biosynthesis
                                                                  structural constituent C, ribosome L38 RPL38
                                                                                          of R        rat
                      Protein component of237 large (60S) ribosomal subunit, has similarity tocytosol ribosomal protein                0
YHR141C     RPL42B    Protein component of762 large (60S) ribosomal subunit, identicalribosome
                                              the protein biosynthesis
                                                                  structural constituent C, R Rpl42Ap and has similarity to rat L44; required for propagati
                                                                                          of to      cytosol    RPL42B                 0
YBR031W     RPL4A     N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E
                                            1089 protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytosol    RPL4A                  0
YPL198W     RPL7B     Protein component of the large (60S) ribosomal subunit, nearly identical cytosol
                                            1551 protein biosynthesis
                                                                  structural constituent C, ribosome Rpl7Ap and has similarity to E. coli L30 and rat L7 rib
                                                                                          of R       to         RPL7B                  0
YLL045C     RPL8B     Ribosomal protein L4771 protein biosynthesis
                                              of the large (60S) ribosomal subunit, nearlyR
                                                                  structural constituent C, ribosome to Rpl8Ap and has similarity to rat L7a ribosomal prot
                                                                                          of identical
                                                                                                     cytosol    RPL8B                  0
YNL067W     RPL9B     Protein component of576 large (60S) ribosomal subunit, nearly identical cytosol
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome Rpl9Ap and has similarity to E. coli L6 and rat L9 ribo
                                                                                          of R       to         RPL9B                  0
YDL081C     RPP1A     Ribosomal protein P1321 translational elongationribosomal stalk,ribosome involved RPP1A
                                              alpha, a component of the constituent C, R
                                                                  structural              of which iscytosol                           0
                                                                                                                in the interaction between translational elonga
YGR214W     RPS0A     Protein component of the small (40S) ribosomal subunit, nearly identical cytosol
                                            1214 protein biosynthesis
                                                                  structural constituent C, ribosome Rps0Bp; required for maturation of 18S rRNA along w
                                                                                          of R        to        RPS0A                  0
YBR048W     RPS11B    Protein component of982 small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 riboso
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytosol    RPS11B                 0
YDR064W     RPS13     Protein component of995 small (40S) ribosomal subunit; has similarity tocytosol S15 and rat S13 ribosomal proteins
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome coli
                                                                                          of R         E.       RPS13                  0
YJL191W     RPS14B    Ribosomal protein 59825 protein biosynthesis
                                              of the small subunit, required for ribosome ribosome and 20S pre-rRNA processing; mutations confer cryp
                                                                  structural constituent C, R
                                                                                          of assemblycytosol    RPS14B                 0
YOL040C     RPS15     Protein component of429 small (40S) ribosomal subunit; has similarity tocytosol S19 and rat S15 ribosomal proteins
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome coli
                                                                                          of R         E.       RPS15                  0
YDL083C     RPS16B    Protein component of864 small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosom
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytosol    RPS16B                 0
YLR441C     RPS1A     Ribosomal protein 10768 protein biosynthesis subunit; nearly C, ribosomeRps1Bp and has similarity to rat S3a ribosomal protein
                                              (rp10) of the small structural constituent of R
                                                                  (40S)                              cytosol
                                                                                          identical to          RPS1A                  0
YHL015W     RPS20     Protein component of366 small (40S) ribosomal subunit; overproduction cytosol
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R                  RPS20                  0
                                                                                                      suppresses mutations affecting RNA polymerase III-dep
YIL069C     RPS24B    Protein component of817 small (40S) ribosomal subunit; identical to Rps24Ap and has similarity to rat S24 ribosomal protein
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytosol    RPS24B                 0
YGR027C     RPS25A                            the protein biosynthesis
                                                                  structural constituent C, ribosome Rps25Bp and has similarity to0rat S25 ribosomal prot
                                                                                          of R
                      Protein component of327 small (40S) ribosomal subunit; nearly identical cytosol to        RPS25A
YHR203C     RPS4B     Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to rat S4 ribosomal protein
                                            1055 protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytosol    RPS4B                  0
YBR181C     RPS6B                           1063 protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytoplasm RPS6B                   0
                      Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein
YBL072C     RPS8A     Protein component of603 small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytosol    RPS8A                  0
YER102W     RPS8B     Protein component of603 small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein
                                              the protein biosynthesis
                                                                  structural constituent C, ribosome
                                                                                          of R       cytosol    RPS8B                  0
YDL100C     ARR4      ATPase, subunit of the GET complex; heat
                                            1065 response to required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependen
                                                                  ATPase activity         E          ER         ARR4                   0
YER026C     CHO1      Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP
                                             831 phosphatidylserine biosynthesis
                                                                  CDP-diacylglycerol-serine O-phosphatidyltransferase activity
                                                                                          E          ER         CHO1                   2
YGR157W     CHO2                            2610 phosphatidylcholine biosynthesis E
                                                                  phosphatidylethanolamine N-methyltransferase activity of phosphatidylethanolamine to p
                                                                                                      step
                      Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first ER in theCHO2       conversion            8
YGL027C     CWH41                           2502 cell I, ER type mannosyl-oligosaccharide glucosidase activityin assembly of cell0wall beta 1,6 glucan an
                                                                   II integral biogenesisN-glycoprotein involved
                      Processing alpha glucosidase wall organization andmembraneE                    ER         CWH41
YHR007C     ERG11                           1593 ergosterol biosynthesis
                                                                  sterol 14-demethylase activity ER
                                                                                          E                     ERG11                  2
                      Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3
YMR202W     ERG2                              catalyzes the biosynthesis isomerase E
                                                                  C-8 sterol              activity ER           delta-7                1
                      C-8 sterol isomerase,669 ergosterolisomerization of the delta-8 double bond to the ERG2 position at an intermediate step in erg
YGL001C     ERG26     C-3 sterol dehydrogenase, catalyzesbiosynthesisof dehydrogenase (C-4 sterol decarboxylase) activity
                                            1050 ergosterol the C-3 sterol three steps required to remove two C-4 methyl groups from an intermediate i
                                                                   second                 E          ER         ERG26                  0
YLR056W     ERG3      C-5 sterol desaturase, catalyzes the biosynthesisof desaturaseE
                                            1098 ergosterol introduction a C-5(6) double bondER episterol, a precursor in ergosterol biosynthesis; m
                                                                  C-5 sterol               activity    into     ERG3                   3
YGL012W     ERG4      C-24(28) sterol reductase,ergosterol biosynthesis in ergosterol biosynthesis; mutants are viable, but lack7
                                            1422 catalyzes the delta24(240) sterol reductase activity
                                                                  final step              E          ER         ERG4                    ergosterol
YDR518W     EUG1                            1554 protein endoplasmic disulfide lumen, functionER
                                                                                          E
                      Protein disulfide isomerase of thefolding proteinreticulum isomerase activity             EUG1                   0
                                                                                                       overlaps with that of Pdi1p; may interact with nascent p
YDR519W     FPR2                             408 unknown          peptidyl-prolyl cis-trans isomerase drugs FK506
                                                                                          E          ER
                      Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase), binds to the activity FPR2 and rapamycin; 0          expression pattern sug
YJL034W     KAR2      ATPase involved in protein import into the ER, also acts as a chaperone ERmediateKAR2 folding in the 1 and may play a role
                                            2049 SRP-dependent cotranslational protein-membrane targeting, translocation
                                                                  ATPase activity         E           to         protein               ER
YMR296C     LCB1                            1677 sphingolipid biosynthesis
                                                                  serine C-palmitoyltransferase activity first committed step in sphingolipid synthesis, wh
                                                                                          E           for the
                      Component of serine palmitoyltransferase, responsible along with Lcb2p membraneLCB1                              0
YHR039C     MSC7      Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic 1
                                            1935 meiotic recombination
                                                                  unknown                 E          ER         MSC7                   reticulum; msc7 mutant
YGL055W     OLE1      Fatty acid desaturase, required for monounsaturated and biogenesisactivity for normal distribution of mitochondria
                                            1533 mitochondrial organization fatty acidE
                                                                  stearoyl-CoA 9-desaturasesynthesisER and      OLE1                   4
YCL043C     PDI1                            1569 protein folding protein disulfide isomerase activity
                                                                                          the        ER         PDI1                   0
                      Protein disulfide isomerase, multifunctional protein resident in E endoplasmic reticulum lumen, essential for the formation of dis
YJL117W     PHO86                            936 protein folding unknown                  E          ER
                      Endoplasmic reticulum (ER) resident protein required for ER exit of the high-affinity PHO86                      2
                                                                                                                 phosphate transporter Pho84p, specifically re
YDL095W     PMT1      Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine res
                                            2454 protein amino acid N-linked glycosylation
                                                                  dolichyl-phosphate-mannose-protein mannosyltransferase activity
                                                                                          E          ER         PMT1                   9
YAL023C     PMT2      Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine res
                                            2280 protein amino acid N-linked glycosylation
                                                                  dolichyl-phosphate-mannose-protein mannosyltransferase activity
                                                                                          E          ER         PMT2                  10
YJR143C     PMT4      Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine res
                                            2289 protein amino acid N-linked glycosylation
                                                                  dolichyl-phosphate-mannose-protein mannosyltransferase activity
                                                                                          E          ER         PMT4                  11
YBR096W     YBR096W                          693 unknown          unknown                 E          ER         YBR096W
                      Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER 0
YBR159W     YBR159W   Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit redu
                                            1044 fatty acid elongation
                                                                  ketoreductase activity  E          ER         YBR159W                1
YDR476C     YDR476C   Hypothetical protein 675 unknown            unknown                 E          ER         YDR476C                0
YNR021W     YNR021W                         1215 unknown
                      Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR
                                                                  unknown                 E          ER         YNR021W                1
YCR011C     ADP1                            3150 transport
                      Putative ATP-dependent permease of the ABC transporter familyCto proteins
                                                                  ATPase activity, coupled of transmembrane movement of substances
                                                                                          E,         cytoplasm,ADP1
                                                                                                                 membrane              8
YDR304C     CPR5                             678 unknown          peptidyl-prolyl cis-trans isomerase activity CPR5
                                                                                          E, C       cytoplasm                         0
                      Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bon
YPR091C     YPR091C                         2313 unknown
                      Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; 1
                                                                  unknown                 E, R       ER         YPR091C                 green fluorescent prote
YDR264C   AKR1                           2295 endocytosis protein-cysteine S-palmitoleyltransferase activity
                                                                                         G            membraneAKR1                       5
                    Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required fo
YEL036C   ANP1                           1503 protein amino acid N-linked glycosylation
                                                                mannosyltransferase activity alpha0,6-mannosyltransferase complex*
                    Subunit of the alpha-1,6 mannosyltransferase complex; type IIG                                ANP1                   1
                                                                                          membrane protein; has a role in retention of glycosyltransferases
YKL135C   APL2      Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; bindsAPL2
                                         2181 vesicle-mediated transport
                                                                clathrin binding         G                          clathrin;            0
                                                                                                      Golgi, endosome? involved in clathrin-dependent Golgi
YPL195W   APL5      Delta adaptin-like subunit vesicle-mediated transport
                                         2799 of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the
                                                                protein binding          G                        APL5
                                                                                                      Golgi, endosome?                   0
YFL048C   EMP47     Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
                                         1338 ER to Golgi transport
                                                                unknown                  G            Golgi       EMP47                  1
YAL007C   ERP2      Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p
                                            648 ER to Golgi transport
                                                                unknown                  G                        ERP2
                                                                                                      COPII-coated vesicle               2
YML012W   ERV25     Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, memberERV25p24 family involved in endoplasmic ret
                                            636 ER to Golgi transport
                                                                unknown                  G                         of vesicle
                                                                                                      COPII-coated the                   1
YOR320C   GNT1      N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the GNT1apparatus
                                         1476 protein amino acid N-linked glycosylation
                                                                acetylglucosaminyltransferase activity
                                                                                         G            Golgi       Golgi                  1
YHL031C   GOS1                              672 in Golgi transport, homolog of the G
                    v-SNARE protein involvedvesicle fusion v-SNARE activity mammalianmembrane, Golgi              GOS1
                                                                                                        protein GOS-28/GS28              1
YGL203C   KEX1      Protease involved in2190processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus o
                                           the protein processing
                                                                carboxypeptidase D activity
                                                                                         G            Golgi       KEX1                   1
YDR483W   KRE2      Alpha1,2-mannosyltransferase of amino acid N-linked glycosylation
                                         1329 protein the Golgi involved in protein G
                                                                alpha0,2-mannosyltransferase activity
                                                                                         mannosylationGolgi       KRE2                   0
YOR099W   KTR1      Alpha-1,2-mannosyltransferase involvedacid N-linked glycosylation glycosylation; member of the KRE2/MNT1 mannosyltransfera
                                         1182 protein amino alpha0,2-mannosyltransferase activity
                                                                 in O- and N-linked protein
                                                                                         G            Golgi       KTR1                   1
YBR205W   KTR3                           1215 cell wall organization and O- and N-linked protein glycosylation;
                                                                mannosyltransferase G
                    Putative alpha-1,2-mannosyltransferase involved in biogenesisactivity membraneKTR3 member of the 1                   KRE2/MNT1 mannosyl
YBR199W   KTR4                           1395 protein amino acid N-linked glycosylation
                                                                   protein glycosylation;activity of the          KTR4
                    Putative mannosyltransferase involved inmannosyltransferase Gmember Golgi KRE2/MNT1 mannosyltransferase family       1
YDR245W   MNN10                          1182 actin filament complex also
                                                                alpha0,6-mannosyltransferase activity Mnn11p, and Hoc1p that mediates elongation o
                                                                                         G            Mnn9p, MNN10
                    Subunit of a Golgi mannosyltransferase organization containing Anp1p, alpha0,6-mannosyltransferase complex           1
YJL183W   MNN11     Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, MNN11 and Hoc1p, 1 mediates elongatio
                                         1269 protein amino acid glycosylation
                                                                alpha0,6-mannosyltransferase activity
                                                                                         G                         Mnn10p,               and
                                                                                                      alpha0,6-mannosyltransferase complex
YBR015C   MNN2      Alpha-1,2-mannosyltransferase, responsible for addition of theG
                                         1794 protein amino acid glycosylation
                                                                alpha0,2-mannosyltransferase activity
                                                                                           first alpha-1,2-linkedMNN2
                                                                                                      Golgi         mannose to form the1 branches on the manna
YJL186W   MNN5      Alpha-1,2-mannosyltransferase, responsible for addition of theG
                                         1761 protein amino acid glycosylation
                                                                alpha0,2-mannosyltransferasealpha-1,2-linked mannose of the branches on the mannan
                                                                                           second activity
                                                                                                      Golgi       MNN5                   1
YGL167C   PMR1                           2853 secretory pathway
                    High affinity Ca2+/Mn2+ P-type ATPase calcium-transporting ATPasetransport into PMR1 involved in Ca2+ dependent protein so
                                                                required for Ca2+ and Mn2+ activity
                                                                                         G            Golgi        Golgi;                7
YCL001W   RER1      Protein involved in retention of membrane proteins, including Sec12p, in COPII-coated vesicle
                                            567 ER to Golgi transport
                                                                unknown                  G                        RER1                   2
                                                                                                      the ER; localized to Golgi; functions as a retrieval recep
YMR071C   TVP18                             504 unknown
                    Integral membrane protein localized to late Golgi vesicles along with the clathrin-coated vesicleinteract with ribosomes, based on
                                                                unknown                  G                        TVP18
                                                                                                      v-SNARE Tlg2p; may                 3
YML115C   VAN1                           1608 protein amino acid N-linked glycosylation
                                                                   forms a complex with  activity membrane, Golgi VAN1                   1
                    Component of the mannan polymerase I;mannosyltransferase GMnn9p, which is involved in in mannan synthesis; mutants are va
YLR309C   IMH1      Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-l
                                         2736 vesicle-mediated transport
                                                                unknown                  G, C         cytosol     IMH1                   0
YPR181C   SEC23                          2307 ER to Golgi of the Sec23p-Sec24p G, C
                                                                GTPase
                    GTPase-activating protein; componenttransport activator activity                  cytoplasm SEC23                    0
                                                                                         heterodimeric complex of the COPII vesicle coat, involved in ER to
YDL058W   USO1                           5373 in intracellular unknown
                                                                protein                  G, C         soluble fraction
                                                                                                                  USO1
                    Essential protein involved ER to Golgi transport transport, coiled-coil protein necessary for transport from0ER to Golgi; required f
YGL054C   ERV14     Protein localized to COPII-coated vesicles, involved in vesicle G, E
                                            417 ER to Golgi transport
                                                                unknown                               ER          ERV14                  3
                                                                                         formation and incorporation of specific secretory cargo; required fo
YML067C   ERV41     Protein localized to COPII-coated vesicles, forms a complex with E
                                         1152 ER to Golgi transport
                                                                unknown                               ER          ERV41                  2
                                                                                         G, Erv46p; involved in the membrane fusion stage of transport; ha
YAL042W   ERV46     Protein localized to COPII-coated vesicles, forms a complex with E
                                         1248 ER to Golgi transport
                                                                unknown                               ER          ERV46                  1
                                                                                         G, Erv41p; involved in the membrane fusion stage of transport
YPR159W   KRE6                           2163 cell wall organization and biogenesis E
                                                                glucosidase activity G,               membraneKRE6                       1
                    Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functionally redundant with Skn1p
YHR181W   SVP26     Integral membrane protein of the early Golgi apparatus and endoplasmicER
                                            687 protein retention in Golgi
                                                                protein binding          G, E                      involved              4
                                                                                                        reticulum,SVP26 in COP II vesicle transport; may als
YNL263C   YIF1      Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and
                                            945 ER to Golgi transport
                                                                unknown                  G, E         ER          YIF1                   5
YKL140W   TGL1                           1647 lipid metabolism    products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and invol
                    Steryl ester hydrolase, one of three genelipase activity             L            membraneTGL1                       0
YMR246W   FAA4                           2085 synthetase, regulates protein modification during growth FAA4 presence of ethanol, functions to incorp
                    Long chain fatty acyl-CoA lipid metabolism                           L, C           activity
                                                                long-chain-fatty-acid-CoA ligasecytoplasm in the                         0
YNL231C   PDR16                           transfer protein (PITP)
                                                                phosphatidylinositol transporter cytoplasm PDR16
                                                                                         L, C         activity                           0
                    Phosphatidylinositol1056 response to drug controlled by the multiple drug resistance regulator Pdr1p, localizes to lipid particles a
YLR380W   CSR1                            transfer protein with phosphatidylinositol transporter activity
                                                                 a potential role in lipid M, C
                                                                                         L,            interacts CSR1                    0
                    Phosphatidylinositol1227 cell wall organization and biogenesis turnover;cytoplasmspecifically with thioredoxin peroxidase (Tsa2p
YKL182W   FAS1                            acid fatty acid biosynthesis
                                                                [acyl-carrier the synthesis C long-chain saturated
                                                                                         L, M, of Mito             activity              0
                    Beta subunit of fatty6156 synthetase, which catalyzesprotein] S-malonyltransferase FAS1 fatty acids; contains acetyltransacyla
YDL193W   NUS1                           1128 unknown           prenyltransferase activitytraffickingER
                    Prenyltransferase, required for cell viability; involved in proteinL, N, E                    NUS1                   1
YHR072W   ERG7                           2196 ergosterol biosynthesis synthase cyclization ER
                                                                lanosterol               L, P,                    ERG7                   0
                    Lanosterol synthase, an essential enzyme that catalyzes the activityE of squalene 2,3-epoxide, a step in ergosterol biosynthes
Q0045     COX1                          12884 oxidase, whichcytochrome-c oxidase activity Mito
                                                                  is the                 M                        COX1                  12
                    Subunit I of cytochrome c aerobic respiration terminal member of the mitochondrial inner membrane electron transport chain; o
Q0250     COX2                              756 oxidase, which is
                                                                cytochrome-c oxidase activity Mito
                                                                                         M                        COX2                   2
                    Subunit II of cytochrome caerobic respirationthe terminal member of the mitochondrial inner membrane electron transport chain;
YAL029C   MYO4                           4416 motors (along microfilament motor activity
                                                                 with MYO2) involved M actin-based transport of cargos; required0for mRNA transport, in
                    One of two type V myosin intracellular mRNA localization             in           Mito        MYO4
YAL039C   CYC3      Cytochrome c heme lyasecytochrome c-hemesynthase), attaches heme Mito
                                            810 (holocytochrome c linkage
                                                                holocytochrome-c synthase activity
                                                                                         M                        CYC3                   0
                                                                                                      to apo-Cyc1p in the mitochondrial intermembrane space
YAL044C   GCV3                              513 one-carbon decarboxylase complex, required for the catabolism
                                                                glycine metabolism M                  Mito        GCV3                   0
                    H subunit of the mitochondrial glycine compounddehydrogenase (decarboxylating) activity of glycine to 5,10-methylene-THF; exp
YBL022C   PIM1                           3402 proteolysis
                    Mitochondrial ATP-dependent protease involved in intramitochondrial proteolysis; involved in degradation0 misfolded proteins in
                                                                ATP-dependent peptidase activity
                                                                                         M            Mito        PIM1                    of
YBL045C   COR1                           1374 aerobic respiration
                                                                ubiquinol-cytochrome-c reductase activity COR1
                                                                                         M            Mito                               0
                    Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner m
YBL064C   PRX1                              786 regulation of with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; induced du
                                                                thioredoxin peroxidase
                    Mitochondrial peroxiredoxin (1-Cys Prx)cell redox homeostasisMactivity Mito                   PRX1                   0
YBL098W   BNA4      Kynurenine 3-mono 1383 NAD biosynthesis biosynthesis of nicotinic acid from tryptophan via kynurenine0
                                          oxygenase, requiredkynurenine 3-monooxygenase activity
                                                                  for                    M            Mito        BNA4                    pathway
YBL099W   ATP1      Alpha subunit of the1638 ATP of mitochondrial F1F0 ATP synthase, synthasea large, evolutionarily conserved enzyme complex r
                                          F1 sector synthesis hydrogen-transporting ATP which is activity, rotational mechanism
                                                                 coupled proton transportM            Mito        ATP1                   0
YBR003W   COQ1                           1422 ubiquinone metabolism
                                                                trans-hexaprenyltranstransferase activity COQ1
                                                                                          in          Mito
                    Hexaprenyl pyrophosphate synthetase, catalyzes the first stepM ubiquinone (coenzyme Q) biosynthesis 0
YBR026C   ETR1      2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where i
                                         1143 aerobic respiration
                                                                enoyl-[acyl-carrier protein] reductase activity
                                                                                         M            Mito        ETR1                   0
YBR039W   ATP3      Gamma subunit of the F1 ATP synthesis hydrogen-transporting ATP synthaseis a large, evolutionarily conserved enzyme comple
                                            936 sector of mitochondrial protonATP synthase, which activity, rotational mechanism
                                                                 coupled F1F0 transport  M            Mito        ATP3                   0
YBR084W   MIS1                           2928 nucleobase, nucleoside, nucleotide andligase activitymetabolism
                                                                formate-tetrahydrofolate nucleic acid
                                                                                         M            Mito        MIS1
                    Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states0of tetrahydrofolate (TH
YBR106W   PHO88                             567 phosphate in phosphate transport; pho88 pho86 double PHO88
                    Probable membrane protein, involvedtransport                         M
                                                                              transporter activity Mito                                  1
                                                                                                                  null mutant exhibits enhanced synthesis of re
YBR120C   CBP6      Protein required for translation of biosynthesis
                                            489 protein the mitochondrial COB mRNA
                                                                unknown                  M            Mito        CBP6                   0
YBR140C   IRA1                           9279 that negatively regulates RAS by converting itMito the GTP- to the GDP-bound inactive form, required
                    GTPase-activating proteinsporulation                                 M
                                                                Ras GTPase activator activity from                IRA1                   0
YBR185C   MBA1      Protein involved in assembly of mitochondrial respiratory complexes; may act as a receptor for proteins destined for export from
                                            837 aerobic respiration
                                                                unknown                  M            Mito        MBA1                   0
YBR227C   MCX1                           1563 unknown
                    Mitochondrial ATP-binding protein, possibly a mitochondrial chaperone with non-proteolytic function; similar to bacterial ClpX pro
                                                                unfolded protein binding M            Mito        MCX1                   0
YBR230C   YBR230C   Integral mitochondrial502 unknown
                                             outer membrane protein; abundance is decreased Mito
                                                                unknown                  M                        YBR230C                0
                                                                                                       in cells grown in glucose relative to other carbon source
YBR263W   SHM1                            hydroxymethyltransferase, involved in one-carbon metabolism
                                                                glycine metabolism M
                    Mitochondrial serine1473 one-carbon compoundhydroxymethyltransferase activity SHM1Mito                               0
YBR269C     FMP21                               417 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP21 purified mitochondria in high-through
                                                                      authentic,            M           Mito      in highly              0
YCL009C     ILV6                                 acetolactate synthase, which catalyzes the first step of
                                                                    enzyme regulator activity
                                                                                            M                     ILV6
                        Regulatory subunit of930 branched chain family amino acid biosynthesisMito branched-chain amino acid 0           biosynthesis; enhances
YCL044C     YCL044C                           1254 unknown
                        Subunit, with Yme1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfo
                                                                    unknown                 M           Mito      YCL044C                0
YCL057C-A   YCL057C-A                           294 unknown           authentic,            M           unknown in highly purified mitochondria in high-through
                        Putative protein of unknown function; theunknown non-tagged protein is detected YCL057C-A                        1
YCL064C     CHA1        Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine o
                                              1083 threonine catabolism aonia-lyase activity
                                                                    L-serine                M           Mito      CHA1                   0
YCR028C-A   RIM1                                491 mitochondrial genome maintenance M
                                                                    single-stranded DNA binding
                        Single-stranded DNA-binding protein essential for mitochondrial genomeMito                RIM1                   0
                                                                                                         maintenance; involved in mitochondrial DNA replication
YCR083W     TRX3        Mitochondrial thioredoxin, response to oxidative stress exchange intermediate activity
                                                384 highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mito
                                                                    thiol-disulfide         M           Mito      TRX3                   0
YDL004W     ATP16       Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme comple
                                                483 ATP synthesis hydrogen-transporting ATP synthase activity, rotational mechanism
                                                                     coupled proton transport
                                                                                            M           Mito      ATP16                  0
YDL066W     IDP1                              1287 glutamate biosynthesis dehydrogenase (NADP+) activityIDP1
                                                                    isocitrate              M           Mito                             0
                        Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required fo
YDL171C     GLT1        NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, fo
                                              6438 glutamate biosynthesis synthase (NADH) activity
                                                                    glutamate               M           Mito      GLT1                   0
YDL174C     DLD1        D-lactate dehydrogenase, aerobic respiration to pyruvate, transcription is Mito
                                              1764 oxidizes D-lactate
                                                                    D-lactate dehydrogenase (cytochrome) activity
                                                                                            M           heme-dependent, repressed by glucose, and derepress
                                                                                                                  DLD1                   0
YDL178W     DLD2                              1593 located in       actin binding
                        D-lactate dehydrogenase, unknown the mitochondrial matrix M                     Mito      DLD2                   0
YDL198C     GGC1                                903 mitochondrial genome nucleotide transporter Mito
                                                                    guanine mitochondrial
                        Mitochondrial GTP/GDP transporter, essential formaintenance M genomeactivity              GGC1                   0
                                                                                                         maintenance; has a role in mitochondrial iron transport;
YDL215C     GDH2        NAD(+)-dependent glutamate dehydrogenase,metabolism
                                              3279 nitrogen compound degrades glutamate activity
                                                                    glutamate dehydrogenase to ammonia and alpha-ketoglutarate; 0
                                                                                            M           Mito      GDH2                    expression sensitive to
YDR019C     GCV1                              1203 one-carbon decarboxylase complex,M
                                                                    glycine metabolism required for the catabolism
                                                                                                        Mito      GCV1                   0
                        T subunit of the mitochondrial glycine compounddehydrogenase (decarboxylating) activity of glycine to 5,10-methylene-THF; exp
YDR036C     EHD3                              1503 endocytosis        indirect role in endocytic membrane trafficking,
                                                                                            M           Mito                             0
                        Protein of unconfirmed function, plays an3-hydroxyisobutyryl-CoA hydrolase activityEHD3member of a family of enoyl-CoA hydra
YDR061W     YDR061W                           1620 unknown
                        Mitochondrial protein, member of the ATP-binding cassette (ABC) to transmembrane movement of substances by Yrm1p along w
                                                                    ATPase activity, coupled transporter family; transcriptionally activated
                                                                                            M           Mito      YDR061W                0
YDR074W     TPS2                              2691 response to stress
                                                                    trehalose-phosphatase activity Mito
                                                                                            M                     TPS2                   0
                        Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate
YDR148C     KGD2        Dihydrolipoyl transsuccinylase, a component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a
                                              1392 tricarboxylic acid cycle
                                                                    dihydrolipoyllysine-residue succinyltransferase activity
                                                                                            M           Mito      KGD2                   0
YDR150W     NUM1        Protein required for nuclear migration, localizes to the mother cell cortex Mito the bud tip; may mediate interactions of dynein and
                                              8247 nuclear migration, microtubule-mediated
                                                                    tubulin binding         M           and       NUM1                   0
YDR194C     MSS116                            1995 RNA splicing RNA splicing factor activity, transesterification mechanism
                                                                                            M           Mito      MSS116                 0
                        DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities
YDR232W     HEM1                              1647 heme biosynthesis
                        5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required
                                                                    5-aminolevulinate synthase activity
                                                                                            M           Mito      HEM1                   0
YDR268W     MSW1                              1140 tryptophanyl-tRNAtryptophan-tRNA ligase
                        Mitochondrial tryptophanyl-tRNA synthetase aminoacylation M activity Mito                 MSW1                   0
YDR298C     ATP5        Subunit 5 of the stator stalk of mitochondrial F1F0protonsynthase, which Mitolarge, evolutionarily conserved enzyme complex req
                                                639 ATP synthesis structural molecule activity
                                                                     coupled ATP transport  M           is a      ATP5                   0
YDR316W     OMS1                              1416 unknown
                        Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory de
                                                                    S-adenosylmethionine-dependent methyltransferase activity
                                                                                            M           Mito      OMS1                   1
YDR322C-A   TIM11       Subunit e of mitochondrialATP synthesis structuralproton transport
                                                291 F1F0-ATPase,coupled molecule activity
                                                                       which is a large, evolutionarily mito
                                                                                            M            conservedTIM11 complex required for ATP synthesis
                                                                                                                   enzyme                0
YDR376W     ARH1                              1482 iron ion homeostasis
                                                                    NADPH-adrenodoxin M     reductase Mito
                                                                                                         activity ARH1                   0
                        Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required f
YDR381C-A   YDR381C-A                           539 unknown          the mitochondrial outer membrane
                        Protein of unknown function, localized to unknown                   M           unknown YDR381C-A                1
YDR430C     CYM1                              2970 proteolysis      metalloendopeptidase activity Mito
                                                                                            M                     CYM1                   0
                        Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins an
YDR529C     QCR7                                384 cytochrome-c reductase complex, which is a component QCR7mitochondrial inner membrane electron
                                                                    ubiquinol-cytochrome-c reductase activity of the
                        Subunit 7 of the ubiquinol aerobic respiration                      M           Mito                             0
YEL020W-A   TIM9                                264 mitochondrial inner membrane protein Mrs11p/Tim10p that mediates import and insertion of a subse
                                                                    unfolded protein bindingwith
                        Mitochondrial intermembrane space protein, forms a complex M import Mito                  TIM9                   0
YEL024W     RIP1                                648 aerobic respiration
                                                                    ubiquinol-cytochrome-c the mitochondrial cytochrome bc1 complex; transfers electrons
                                                                                            M           Mito
                        Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein ofreductase activity RIP1                         0
YEL052W     AFG1        Putative ATPase of 1530 unknown
                                              the CDC48/PAS1/SEC18 (AAA) family, localized to mitochondria
                                                                    ATPase activity         M           Mito      AFG1                   0
YER014W     HEM14       Protoporphyrinogen 1620 hememitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converti
                                               oxidase, a biosynthesis
                                                                    protoporphyrinogen oxidase activity
                                                                                            M           Mito      HEM14                  0
YER017C     AFG3        Component, with Yta12p, protein complex assemblymembrane m-AAA protease that mediates degradation of misfolded or unas
                                              2286 of the mitochondrial inner
                                                                    ATPase activity         M           Mito      AFG3                   1
YER069W     ARG5,6      Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductas
                                              2592 regulation of transcription
                                                                    N-acetyl-gaMMa-glutamyl-phosphate reductase activity
                                                                                            M           Mito      ARG5,6                 0
YER073W     ALD5        Mitochondrial aldehyde dehydrogenase, involved in regulation M biosynthesis of electron transport chain 0
                                              1563 electron transport
                                                                    aldehyde dehydrogenase activity
                                                                                            or          Mito      ALD5                   components and acetat
YER078C     YER078C                           1536 proteolysis      metalloendopeptidase activity Mito
                        Metallopeptidase, localized to the mitochondrial matrix             M                     YER078C                0
YER080W     FMP29                             1884 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP29 purified mitochondria in high-through
                                                                      authentic,            M           Mito      in highly              0
YER086W     ILV1                              1731 branched firstthreonine aMMonia-lyase activityexpression is under general amino acid control; ILV1 lo
                                                                       family amino acid biosynthesis
                                                                                            M
                        Threonine deaminase, catalyzes thechainstep in isoleucine biosynthesis;Mito               ILV1                   0
YER087W     YER087W                           1731 unknown          proline-tRNA ligase activity
                                                                                            M           unknown YER087W                  0
                        Protein with similarity to tRNA synthetases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-th
YER154W     OXA1                              1209 mitochondrial inner membrane protein insertion of
                                                                    protein transporter activity
                                                                                            M                     OXA1                   1
                        Translocase of the mitochondrial inner membrane, mediates the import Mitoboth mitochondrial- and nuclear-encoded proteins fr
YER155C     BEM2                              6504 cell wall organization transducer activity
                                                                    signal and biogenesis   M           Mito      BEM2
                        Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular0morphogenesis; requir
YER178W     PDA1        E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to ac
                                              1263 pyruvate metabolism dehydrogenase (acetyl-transferring) activity
                                                                    pyruvate                M           Mito      PDA1                   0
YER182W     FMP10                               735 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP10 purified mitochondria in high-through
                                                                      authentic,            M           Mito      in highly              1
YFL016C     MDJ1                              1536 of mitochondrially synthesized binding   M           Mito      MDJ1                    the
                        Protein involved in folding protein folding unfolded protein proteins in the mitochondrial matrix; localizes to0 mitochondrial inner
YFL018C     LPD1        Dihydrolipoamide dehydrogenase, the lipoamidedehydrogenase (decarboxylating) of the pyruvate dehydrogenase and 2-oxogluta
                                              1500 glycine catabolism
                                                                    glycine dehydrogenase component (E3) activity
                                                                                            M           Mito      LPD1                   0
YGL018C     JAC1        Specialized J-protein 555 aerobic respiration in Fe-S clusterM
                                                that functions with Hsp70
                                                                    chaperone binding                   Mito      JAC1                   0
                                                                                             biogenesis in mitochondria, involved in iron metabolism; contains
YGL080W     FMP37                               393 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP37 purified mitochondria in high-through
                                                                      authentic,            M           Mito      in highly              2
YGL107C     RMD9                              1941 unknown
                        Mitochondrial protein required for sporulation
                                                                    unknown                 M           Mito      RMD9                   0
YGL119W     ABC1        Protein required for ubiquinone (coenzyme Q) biosynthesis and for respiratory growth; exhibits genetic interaction with COQ9, su
                                              1506 aerobic respiration
                                                                    unknown                 M           Mito      ABC1                   0
YGL143C     MRF1        Mitochondrial polypeptide protein biosynthesis involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond durin
                                              1242 chain release factor,
                                                                    translation release factor activity
                                                                                            M           Mito      MRF1                   0
YGL187C     COX4                                468 aerobic respiration
                                                                    cytochrome-c oxidase activity mitochondrial inner membrane electron transport chain
                                                                                            M
                        Subunit IV of cytochrome c oxidase, which is the terminal member of theMito               COX4                   0
YGR012W     YGR012W                           1182 unknown
                        Putative cysteine synthase, localized to the mitochondrial outer membrane
                                                                    cysteine synthase activity
                                                                                            M           Mito      YGR012W                1
YGR029W     ERV1                                653 mitochondrial organization and biogenesis
                                                                    thiol oxidase activity M            Mito      ERV1
                        Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part0of a disulfide relay sys
YGR031W     YGR031W                           1029 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YGR031W
                                                                      authentic,            M           Mito                             0
                                                                                                                  in highly purified mitochondria in high-through
YGR049W     SCM4                                564 cell CDC4
                        Potential regulatory effector ofcycle function, suppresses a temperature-sensitive SCM4of CDC4, tripartite protein structure in w
                                                                    unknown                 M           Mito      allele                 4
YGR052W     FMP48                             1110 unknown
                        Putative protein of unknown function; thekinase activity
                                                                      authentic, non-tagged protein is detected FMP48 purified mitochondria in high-through
                                                                                            M           Mito      in highly              0
YGR082W     TOM20       Component of the TOM (translocase of protein import
                                                552 mitochondral outer membrane) complex responsible for recognition and initial import steps for all mitoc
                                                                    protein transporter activity
                                                                                            M           Mito      TOM20                  1
YGR132C     PHB1                             864 protein folding unknown                    M          Mito        PHB1                   0
                      Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilize
YGR181W     TIM13                            318 mitochondrial inner membrane protein TIm8p that
                                                                  protein transporter activity
                                                                                            with                   TIM13                  0
                      Mitochondrial intermembrane space protein, forms a complex M import Mito mediates import and insertion of a subset of polyto
YGR193C     PDX1      Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP)M the mitochondrial pyruvate dehydrogenase (PDH) complex, play
                                            1233 acetyl-CoA biosynthesis
                                                                  protein binding            of        Mito        PDX1                   0
YGR207C     YGR207C   Protein that interacts 786 unknown
                                             with frataxin (Yfh1p); homolog of mammalian electron transferYGR207C complex 0
                                                                  unknown                   M          Mito         flavoprotein           subunit ETF-beta; the a
YGR231C     PHB2                             933 protein folding unknown                    M          Mito        PHB2                   1
                      Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilize
YGR244C     LSC2      Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of MitoTCA cycle that catalyzes the0
                                            1284 tricarboxylic acid cycle
                                                                  succinate-CoA ligaseM                the
                                                                                             (ADP-forming) activityLSC2                    nucleotide-dependent c
YGR255C     COQ6      Putative flavin-dependent ubiquinone metabolism biosynthesis monooxygenase biosynthesis; located on the matrix side of the
                                            1440 monooxygenase, involved in ubiquinone (Coenzyme Q) activity
                                                                  ubiquinone                M          Mito        COQ6                   0
YHL021C     FMP12                           1398 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP12 purified mitochondria in high-through
                                                                    authentic,              M          Mito        in highly              0
YHR003C     YHR003C                         1290 unknown
                      Protein of unknown function, localized to unknown
                                                                   the mitochondrial outer membrane
                                                                                            M          Mito        YHR003C                0
YHR005C-A   MRS11                            282 mitochondrial inner membrane protein import Mito
                                                                  unfolded protein binding  M                      MRS11                  0
                      Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that mediates insertio
YHR008C     SOD2                             702 replicative protects cells against oxygen toxicity
                                                                  aging                     M          Mito
                      Mitochondrial superoxide dismutase; cell manganese superoxide dismutase activity SOD2                               0
YHR017W     YSC83                           1158 unknown
                      Non-essential mitochondrial protein of unknown function; mRNA inducedMito
                                                                  unknown                   M                      YSC83                  0
                                                                                                        during meiosis, peaking between mid to late prophase o
YHR024C     MAS2      Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targetin
                                            1449 mitochondrial protein processing
                                                                  mitochondrial processing peptidase activity
                                                                                            M          Mito        MAS2                   0
YHR037W     PUT2      Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole ni
                                            1728 glutamate biosynthesis
                                                                  1-pyrroline-5-carboxylate dehydrogenase activity
                                                                                            M          Mito        PUT2                   0
YHR076W     PTC7                            1125 unknown
                      Mitochondrially localized type 2C protein protein phosphatase type 2C activity growth on ethanol and by sustained osmotic stress
                                                                   phosphatase; expression induced by
                                                                                            M          Mito        PTC7                   1
YHR100C     YHR100C                          558 unknown          unknown                   M           authentic, non-tagged
                      Putative protein of unknown function, required for respiratory growth; the unknown YHR100C protein is 0              detected in highly purifi
YHR106W     TRR2      Mitochondrial thioredoxin reductase to oxidative protection against oxidative stress, required with Glr1p to 0
                                            1029 response involved in stress
                                                                  thioredoxin-disulfide reductase activity
                                                                                            M          Mito        TRR2                    maintain the redox state
YHR117W     TOM71                           1920 unknown
                      Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer me
                                                                  protein transporter activity
                                                                                            M          Mito        TOM71                  0
YHR198C     FMP22                            966 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP22 purified mitochondria in high-through
                                                                    authentic,              M          Mito        in highly              0
YHR199C     FMP34                            933 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP34 purified mitochondria in high-through
                                                                    authentic,              M          Mito        in highly              0
YHR208W     BAT1                            1182 branched acid family amino acid homolog of murine
                                                                  branched-chain-amino-acid transaminase activity
                                                                                            M                      BAT1                   0
                      Mitochondrial branched-chain aminochain aminotransferase, biosynthesisMito ECA39; highly expressed during logarithmic pha
YIL022W     TIM44     Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the tra
                                            1296 mitochondral protein import
                                                                  protein transporter activity
                                                                                            M          Mito        TIM44                  0
YIL043C     CBR1      Microsomal cytochrome b electron transport
                                             969 reductase, notcytochrome-b5 reductase activity in mitochondria; mutation in1
                                                                    essential for viability;M          Mito
                                                                                              also detected        CBR1                    conserved NADH bindi
YIL051C     MMF1                             involved in maintenance of the mitochondrial
                                                                  unknown
                      Mitochondrial protein 438 mitochondrial genome maintenance M genome              Mito        MMF1                   0
YIL070C     MAM33     Acidic protein of the mitochondrialrespiration
                                             801 aerobic matrixunknown in oxidative M
                                                                    involved                           Mito        MAM33                  0
                                                                                            phosphorylation; related to the human complement receptor gC1q
YIL098C     FMC1      Mitochondrial matrix protein, required for unknown or stability M high temperature of the F1 sector of mitochondrial F1F0 ATP sy
                                             468 protein complex assembly
                                                                   assembly                 at         Mito        FMC1                   0
YIL157C     FMP35                            594 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP35 purified mitochondria in high-through
                                                                    authentic,              M          Mito        in highly              0
YJL045W     YJL045W   Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and
                                            1905 cellular respiration
                                                                  succinate dehydrogenase (ubiquinone) activity
                                                                                            M          Mito        YJL045W                0
YJL054W     TIM54                           1437 mitochondrial inner membrane protein import Mito
                                                                  protein complex involved  M                      TIM54                  0
                      Component of the mitochondrial Tim54p-Tim22ptransporter activity in insertion of polytopic proteins into the inner membrane
YJL066C     MPM1                             759 unknown
                      Mitochondrial membrane protein of unknown function, contains no hydrophobic stretches
                                                                  unknown                   M          Mito        MPM1                   0
YJL104W     PAM16     Constituent of the mitochondrial import motor associated with M presequence translocase, along with Ssc1p, Tim44p, Mge1p, a
                                             450 mitochondral protein import
                                                                  protein binding           the        Mito        PAM16                  0
YJL143W     TIM17     Essential constituent 477 mitochondral protein import
                                             of the mitochondrial inner membrane presequence Mito
                                                                  protein transporter activity
                                                                                            M                      TIM17                  3
                                                                                                       translocase; interacts with Pam18p to recruit the preseq
YJL200C     YJL200C                         2370 unknown
                      Putative mitochondrial aconitase isozyme; similarity to Aco1p, M aconitase required for the TCA cycle; expression induced durin
                                                                  aconitate hydratase activity
                                                                                             an        Mito        YJL200C                0
YJR048W     CYC1                             330
                      Cytochrome c, isoform 1; electron carrierelectron carrier activity
                                                            transport the mitochondrial M
                                                                    of                                 Mito        CYC1                   0
                                                                                            intermembrane space that transfers electrons from ubiquinone-cyt
YJR051W     OSM1                            1506 metabolism fumarate reductase (NADH) activity
                                                                                            M          Mito        OSM1                   1
                      Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under an
YJR077C     MIR1                             936 phosphate transport
                                                                   inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abun
                                                                                            M
                      Mitochondrial phosphate carrier, imports inorganic phosphate transporterMito      activity MIR1                     0
YJR085C     YJR085C                          318 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YJR085C
                                                                    authentic,              M          Mito                               3
                                                                                                                   in highly purified mitochondria in high-through
YJR100C     YJR100C                          984 unknown          unknown                   M          unknown YJR100C                    0
                      Putative protein of unknown function; appears related to murine NOR1, which is highly expressed in leukemogenic cell lines; the
YJR121W     ATP2      Beta subunit of the F1 sector of mitochondrial F1F0 ATP transport which is a activity, rotational mechanism enzyme complex re
                                            1536 ATP synthesis hydrogen-transporting ATP synthase large, evolutionarily conserved
                                                                   coupled proton synthase, M          Mito        ATP2                   0
YJR122W     CAF17                           1494 interacts
                      Mitochondrial protein that unknown with Ccr4p in the two-hybrid system; 3'-untranslated region contains a0
                                                                  unknown                   M          Mito        CAF17                   putative mRNA localiza
YJR135W-A   TIM8                             264 mitochondrial inner membrane protein import Mito
                                                                  protein transporter activity
                                                                                            M                      TIM8                   0
                      Mitochondrial intermembrane space protein mediating import and insertion of polytopic inner membrane proteins; homolog of hum
YJR144W     MGM101                           810 DNA repair
                      Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxida
                                                                  DNA binding               M          Mito        MGM101                 0
YKL016C     ATP7      Subunit d of the stator stalk of mitochondrial F1F0 molecule activity
                                             525 protein complex assembly
                                                                  structural ATP synthase, which Mitolarge, evolutionarily conserved enzyme complex req
                                                                                            M          is a        ATP7                   0
YKL027W     YKL027W                         1344 unknown
                      Protein of unknown function, localized to unknown
                                                                   the mitochondrial outer membrane
                                                                                            M          Mito        YKL027W                1
YKL029C     MAE1                            2010 pyruvate metabolismenzyme activityM
                                                                  malic                                Mito        MAE1                   0
                      Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar m
YKL040C     NFU1      Protein involved in iron metabolism in mitochondria; similar to M
                                             771 iron ion homeostasis
                                                                  unknown                              Mito        NFU1                   0
                                                                                            NifU, which is a protein required for the maturation of the Fe/S clus
YKL085W     MDH1      Mitochondrial malate dehydrogenase, catalyzes interconversion of activity and oxaloacetate; involved in the tricarboxylic acid (TC
                                            1005 tricarboxylic acid cycle dehydrogenase malate
                                                                  L-malate                  M          Mito        MDH1                   0
YKL087C     CYT2      Cytochrome c1 heme675 cytochromein maturation of cytochrome c1,activity is a subunit of the mitochondrial ubiquinol-cytochrom
                                              lyase, involved c-heme linkage
                                                                  holocytochrome-c synthase which
                                                                                            M          Mito        CYT2                   0
YKL120W     OAC1                             975 sulfate transport
                      Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, and thiosulfate; member of the mitochondrial carrier
                                                                  oxaloacetate carrier activity
                                                                                            M          Mito        OAC1                   1
YKL192C     ACP1                             378 fatty acid biosynthesis in activity M
                                                                  involved                             Mito        ACP1                   0
                      Mitochondrial matrix acyl carrier protein, acyl carrier biosynthesis of octanoate, which is a precursor to lipoic acid; activated by ph
YKL195W     MIA40                           1212 protein targeting
                      Essential protein of the mitochondrial intermembrane space (IMS); promotes retention of newly imported 1
                                                                  unknown                   M          Mito        MIA40                   proteins; may do so by
YKR016W     FMP13                           1623 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP13 purified mitochondria in high-through
                                                                    authentic,              M          Mito        in highly              1
YKR065C     PAM17     Presequence translocase-associated motor subunit, required for stable complex formation between cochaperones Pam16p and
                                             594 mitochondral protein import
                                                                  unknown                   M          Mito        PAM17                  2
YKR066C     CCP1      Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen activity in mitochondria, involved in the response to oxidative
                                            1086 response to oxidative stress peroxidase species
                                                                  cytochrome-c              M          Mito        CCP1                   1
YKR070W     YKR070W                         1059 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YKR070W
                                                                    authentic,              M          Mito                               0
                                                                                                                   in highly purified mitochondria in high-through
YLL001W     DNM1      Dynamin-related GTPase mitochondrial organizationfission and the maintenance ofDNM1
                                            2274 required for mitochondrial and biogenesis
                                                                  GTPase activity           M          Mito                               0
                                                                                                                    mitochondrial morphology, assembles on the
YLL041C     SDH2      Iron-sulfur protein subunit tricarboxylic acid cycle dehydrogenase (ubiquinone)Sdh4p), which couples the oxidation of succinate
                                             801 of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, activity
                                                                  succinate                 M          Mito        SDH2                   0
YLR008C     PAM18     Constituent of the mitochondrial import motor associated with M presequence translocase, along with Ssc1p, Tim44p, Mge1p, a
                                             507 mitochondral protein import
                                                                  unfolded protein binding  the        Mito        PAM18                  0
YLR038C     COX12                            252 cytochrome c oxidase complex assembly
                                                                  cytochrome-c oxidase activity Mito
                                                                                            M                      COX12
                      Subunit VIb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane0electron transport chai
YLR059C     REX2      RNA exonuclease, required for processing maturation; functions redundantly with Rnh70p in 5.8S rRNA maturation, and with Rnh
                                             810 RNA U4 snRNA exonuclease activity
                                                                  3'-5'                    M          Mito        REX2                   0
YLR069C     MEF1      Mitochondrial elongation factor involved in translational elongation activity
                                           2286 translational elongation elongationM
                                                                  translation               factor    Mito        MEF1                   0
YLR077W     FMP25                          1752 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP25 purified mitochondria in high-through
                                                                    authentic,             M          Mito        in highly              1
YLR089C     ALT1                           1779 unknown           pyruvic transaminase);              Mito        ALT1                   0
                      Putative alanine transaminase (glutamic transaminase activityM the authentic, non-tagged protein is detected in highly purified m
YLR163C     MAS1      Smaller subunit of the mitochondrial processingprocessing
                                           1389 mitochondrial protein protease (MPP), essential processing enzyme that cleaves the N-terminal target
                                                                  mitochondrial processing peptidase activity
                                                                                           M          Mito        MAS1                   0
YLR203C     MSS51     Nuclear encoded protein requiredbiosynthesis of COX1 mRNA; binds to Cox1 protein
                                           1311 protein for translation
                                                                  unknown                  M          Mito        MSS51                  0
YLR290C     YLR290C                          834 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YLR290C
                                                                    authentic,             M          Mito                               0
                                                                                                                  in highly purified mitochondria in high-through
YLR291C     GCD7      Beta subunit of the translation initiationinitiation
                                           1146 translational factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regu
                                                                  translation initiation factor activity
                                                                                           M          Mito        GCD7                   0
YLR295C     ATP14     Subunit h of the F0 sectorATP synthesis hydrogen-transporting ATP synthase activity, rotationalconserved enzyme complex requ
                                             375 of mitochondrial F1F0 ATP synthase, which isMito
                                                                   coupled proton transportM                      ATP14
                                                                                                        a large, evolutionarily mechanism0
YLR348C     DIC1      Mitochondrial dicarboxylate carrier, integral transport acid transporter activity
                                             897 dicarboxylic acid membrane protein, catalyzesMito
                                                                  dicarboxylic             M                      DIC1                   0
                                                                                                        a dicarboxylate-phosphate exchange across the inner m
YLR369W     SSQ1                           1974 DNA-dependent DNA required for M
                                                                  unfolded protein binding
                      Mitochondrial hsp70-type molecular chaperone, replication assembly ofMito                    clusters               at
                                                                                                       iron/sulfurSSQ1 into proteins 0 a step after cluster s
YLR454W     FMP27                          7887 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP27 purified mitochondria in high-through
                                                                    authentic,             M          Mito        in highly              0
YML054C     CYB2                           1776 cytochrome-c oxidoreductase), component of the mitochondrial intermembrane0space, required for lac
                      Cytochrome b2 (L-lactate electron transport                          M          Mito
                                                                  L-lactate dehydrogenase (cytochrome) activity   CYB2
YML072C     TCB3                           4638 unknown
                      Lipid-binding protein, localized to the budlipid specific mRNA transport; non-tagged protein localizes to mitochondria and GFP-fus
                                                                    via binding            M          Mito        TCB3                   1
YML078W     CPR3                             549 protein folding peptidyl-prolyl cis-trans catalyzes the cis-trans isomerization of peptide bonds N-terminal t
                                                                                                      Mito
                      Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), M isomerase activity CPR3                         0
YML081C-A   ATP18     Subunit of the mitochondrial F1F0 ATP synthase, proton is a large enzyme complexATP18 for ATP synthesis; termed subunit I
                                             180 ATP synthesis hydrogen-transporting ATP synthase activity, rotational mechanism
                                                                   coupled which transport M          Mito         required              0
YML086C     ALO1                           1581 response to oxidative final step in biosynthesisMito
                                                                  D-arabinono0,4-lactone oxidase activity ALO1
                      D-Arabinono-1,4-lactone oxidase, catalyzes the stress                M                                             0
                                                                                                        of D-erythroascorbic acid, which is protective against ox
YML110C     COQ5      2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase,M
                                             924 aerobic respiration
                                                                  2-hexaprenyl-6-methoxy0,4-benzoquinone COQ5
                                                                                            involved in ubiquinone (Coenzyme Q) biosynthesis; located in mit
                                                                                                      Mito                               0
                                                                                                                   methyltransferase activity
YML120C     NDI1                           1542 mitochondrial electron transport, NADH to ubiquinone orthe respiratory chain but does not pumpas ac
                                                                  oxidoreductase activity, acting on NADH NADPH, quinone or similar compound proto
                                                                                           M          Mito
                      NADH:ubiquinone oxidoreductase, transfers electrons fromNADH to ubiquinone in NDI1                                 1
YMR062C     ECM40                          1326 cell wall organization and the fifth step
                                                                  amino-acid N-acetyltransferase Mito
                                                                                           M           activity   ECM40
                      Mitochondrial ornithine acetyltransferase, catalyzes biogenesis in arginine biosynthesis; also possesses0acetylglutamate syntha
YMR066W     SOV1                           2697 unknown
                      Mitochondrial protein of unknown function   unknown                  M          Mito        SOV1                   0
YMR072W     ABF2                             552 mitochondrial genome maintenance M replication and recombination, member0 HMG1 DNA-binding
                                                                  DNA mitochondrial DNA
                      Mitochondrial DNA-binding protein involved in binding                           Mito        ABF2                    of
YMR115W     FMP24                          1506 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP24 purified mitochondria in high-through
                                                                    authentic,             M          Mito        in highly              1
YMR145C     NDE1                           1683 ethanol fermentation dehydrogenase activity cytosolic NADH; Nde1p and Nde2p are involved in provi
                                                                  NADH                     M
                      Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of Mito                  NDE1                   0
YMR189W     GCV2                           3105 one-carbon decarboxylase complex,M
                                                                  glycine metabolism required for the catabolism
                                                                                                      Mito        GCV2                   0
                      P subunit of the mitochondrial glycine compounddehydrogenase (decarboxylating) activity of glycine to 5,10-methylene-THF; exp
YMR207C     HFA1                           6372 fatty A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis
                                                                  acetyl-CoA carboxylase activity Mito
                      Mitochondrial acetyl-coenzymeacid biosynthesis                       M                      HFA1                   0
YMR212C     EFR3      Non-essential protein of unknown morphogenesis synthetic lethal genetic interactions with PHO85; the authentic, non-tagged p
                                           2349 cellular function; exhibits
                                                                  unknown                  M          Mito        EFR3                   0
YMR267W     PPA2                             933 aerobic respiration
                                                                  inorganic for mitochondrial function
                                                                                           M                      PPA2                   0
                      Mitochondrial inorganic pyrophosphatase, requireddiphosphatase activityMito and possibly involved in energy generation from ino
YMR287C     MSU1      RNase, component of the RNA catabolism
                                           2910 mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome as
                                                                  exoribonuclease II activity
                                                                                           M          Mito        MSU1                   0
YMR301C     ATM1      Mitochondrial inner membraneion homeostasis activity, coupled to transmembrane movement of substances clusters to the cy
                                           2073 iron transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S)
                                                                  ATPase                   M          Mito        ATM1                   5
YMR302C     PRP12                          2553 rRNA processing   exonuclease activity M              Mito        PRP12                  0
                      Integral inner mitochondrial membrane protein with similarity to exonucleases; mutants exhibit an increased rate of mitochondria
YNL026W     SAM50     Essential component of the Sorting and Assembly Machinery (SAM or Tob complex) of the mitochondrial 0
                                           1455 protein complex assembly
                                                                  unknown                  M          Mito        SAM50                   outer membrane, which
YNL037C     IDH1                           1083 tricarboxylic acid cycle dehydrogenase (NAD+) activity IDH1
                                                                  isocitrate               M          Mito                               0
                      Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglu
YNL071W     LAT1                           1449 pyruvate component
                      Dihydrolipoamide acetyltransferasemetabolism (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarbo
                                                                  dihydrolipoyllysine-residue acetyltransferase activity
                                                                                           M          Mito        LAT1                   0
YNL121C     TOM70     Component of the TOM (translocase of protein import
                                           1854 mitochondral outer membrane) complex responsible for recognition and initial import steps for all mitoc
                                                                  protein transporter activity
                                                                                           M          Mito        TOM70                  1
YNL131W     TOM22     Component of the TOM (translocase of protein import
                                             459 mitochondral outer membrane) complex responsible for initial import of mitochondrially directed protein
                                                                  protein transporter activity
                                                                                           M          Mito        TOM22                  0
YNL168C     FMP41                            780 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP41 purified mitochondria in high-through
                                                                    authentic,             M          Mito        in highly              0
YNL169C     PSD1                           1503 phosphatidylcholine biosynthesis membrane,Mito
                                                                   mitochondrial inner M               converts PSD1                     0
                      Phosphatidylserine decarboxylase of the phosphatidylserine decarboxylase activityphosphatidylserine to phosphatidylethanolamin
YNL310C     ZIM17                            618 zinc finger motif; essential for          M          Mito        ZIM17                  0
                      Heat shock protein with a protein folding protein binding protein import into mitochondria; may act with Pam18p to facilitate recog
YNL315C     ATP11     Molecular chaperone,957 protein complex assembly
                                              required for the assembly ofprotein and beta subunits into theATP11
                                                                  unfolded alpha binding   M          Mito                               0
                                                                                                                   F1 sector of mitochondrial F1F0 ATP syntha
YNL320W     YNL320W                          855 unknown            authentic,             M          unknown in highly purified mitochondria in high-through
                      Putative protein of unknown function; theunknown non-tagged protein is detected YNL320W                            2
YNR001C     CIT1      Citrate synthase, catalyzes the condensation of(Si)-synthase activity
                                           1440 tricarboxylic acid cycleacetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of t
                                                                  citrate                  M          Mito        CIT1                   0
YNR017W     MAS6                             669 mitochondral protein import
                                                                  protein transporter activity
                                                                                           M          Mito        MAS6
                      Essential protein of the mitochondrial inner membrane, component of the mitochondrial import system 0
YNR040W     YNR040W                          771 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YNR040W
                                                                    authentic,             M          Mito                               0
                                                                                                                  in highly purified mitochondria in high-through
YOL027C     MDM38     Mitochondrial inner membrane protein, involved in membrane M
                                           1722 mitochondrial organization and biogenesis
                                                                  unknown                               of a      MDM38                  1
                                                                                           integrationMito subset of mitochondrial proteins; required for K+/H
YOL042W     NGL1                           1092 unknown           endonuclease activityM              Mito        NGL1                   0
                      Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the
YOL071W     EMI5      Non-essential protein489 sporulation
                                              of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IM
                                                                  unknown                  M          Mito        EMI5                   0
YOL140W     ARG8      Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
                                           1272 arginine biosynthesis
                                                                  acetylornithine transaminase activity
                                                                                           M          Mito        ARG8                   0
YOR020C     HSP10                            321 protein folding unfolded protein binding  M          Mito        HSP10                  0
                      Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein fo
YOR090C     PTC5                           1719 unknown
                      Mitochondrially localized type 2C protein protein phosphatase typeregulation of pyruvate dehydrogenase activity; contains Mg2+/M
                                                                   phosphatase involved in 2C activity
                                                                                           M          Mito        PTC5                   0
YOR108W     LEU9      Alpha-isopropylmalate synthase IIbiosynthesis
                                           1815 leucine (2-isopropylmalate synthase), catalyzes the first step in the leucine biosynthesis pathway; the
                                                                  2-isopropylmalate synthase activity
                                                                                           M          Mito        LEU9                   0
YOR125C     CAT5      Mitochondrial inner membrane proteinmetabolism
                                             702 ubiquinone directly involved in ubiquinone biosynthesis, CAT5
                                                                  unknown                  M          Mito                               0
                                                                                                                  essential for several other metabolic pathway
YOR136W     IDH2                           1110 tricarboxylic acid cycle dehydrogenase (NAD+) activity IDH2
                                                                  isocitrate               M          Mito                               0
                      Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglu
YOR142W     LSC1      Alpha subunit of succinyl-CoA ligase, whichcyclemitochondrialM
                                             990 tricarboxylic acid is a
                                                                  succinate-CoA ligase (ADP-forming) TCA cycle that catalyzes the nucleotide-dependent
                                                                                            enzyme of the activity
                                                                                                      Mito        LSC1                   0
YOR176W     HEM15     Ferrochelatase, a mitochondrial biosynthesis
                                           1182 heme inner membrane protein, catalyzes the insertion of HEM15 iron into protoporphyrin IX, the eighth
                                                                  ferrochelatase activity  M          Mito        ferrous                0
YOR187W     TUF1                           1314 translational elongation activity
                                                                  GTPase
                      Mitochondrial translation elongation factor Tu; comprises both M                Mito        TUF1
                                                                                            GTPase and guanine nucleotide exchange0factor activities, while t
YOR188W     MSB1      Protein involved in positive regulation of both 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway, potential Cdc28p substra
                                           3414 establishment of cell polarity
                                                                  unknown                  M          Mito        MSB1                   0
YOR196C     LIP5                           1245 lipoic of the coenzyme synthase activity
                                                                  lipoic acid              M          Mito         lipoic
                      Protein involved in biosynthesis acid biosynthesis lipoic acid, has similarity to E. coliLIP5 acid synthase 0
YOR211C     MGM1      Mitochondrial GTPase related to dynamin, present in and biogenesis
                                           2709 mitochondrial organization a complex containing Ugo1p and Fzo1p; required for normal morphology of
                                                                  GTPase activity          M          Mito        MGM1                   0
YOR215C     YOR215C                          558 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YOR215C
                                                                    authentic,            M          Mito                               0
                                                                                                                in highly purified mitochondria in high-through
YOR221C     MCT1                            1356 aerobic respiration
                      Predicted malonyl-CoA:ACP transferase,[acyl-carrier protein] S-malonyltransferase MCT1 acid synthase that produces interme
                                                                    putative component M a type-IIMito
                                                                                          of                    activity
                                                                                                       mitochondrial fatty              0
YOR228C     YOR228C                          909 unknown
                      Protein of unknown function, localized to unknown
                                                                   the mitochondrial outer membrane
                                                                                          M          Mito       YOR228C                 5
YOR232W     MGE1      Protein of the mitochondrial matrix involved in protein import into mitochondria; actsMGE1cochaperone and a nucleotide release f
                                             687 mitochondral protein import
                                                                  unfolded protein bindingM          Mito         as a                  0
YOR271C     YOR271C   Putative protein, predictedunknown
                                             984 by computational methods to be anM
                                                                  unknown                            Mito       YOR271C                 4
                                                                                           alpha-isopropylmalate carrier; the authentic, non-tagged protein is
YOR286W     FMP31                            450 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP31 purified mitochondria in high-through
                                                                    authentic,            M          Mito       in highly               0
YOR330C     MIP1                            3843 DNA-dependent polymerase; conserved C-terminal segment is required for the0maintenance of mitoch
                                                                  gaMMa DNA-directedM
                      Catalytic subunit of the mitochondrial DNA DNA replication                     Mito       MIP1
                                                                                           DNA polymerase activity
YOR354C     MSC6                            2079 meiotic recombination
                                                                  unknown                 M          Mito       MSC6
                      Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous0chromatids; the authen
YOR356W     YOR356W   Mitochondrial protein with NAD catabolism
                                            1896 similarity to flavoprotein-type oxidoreductases;Mito CH-NH group of donors, quinone or with other mito
                                                                  oxidoreductase activity, acting on the in aYOR356W
                                                                                          M           found      large supramolecular0   complex similar compo
YOR374W     ALD4                            1560 ethanol metabolism
                                                                  aldehyde dehydrogenase (NAD)Mito
                                                                                          M            activity ALD4                    0
                      Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; activity is K+
YPL059W     GRX5      Hydroperoxide and superoxide-radical osmotic stress exchange intermediate activity
                                             453 response to responsive glutathione-dependentMito
                                                                  thiol-disulfide         M                     GRX5                    0
                                                                                                      oxidoreductase; mitochondrial matrix protein involved in
YPL063W     TIM50     Constituent of the mitochondrial inner membrane presequence translocase (TIM23 TIM50
                                            1431 mitochondral protein import
                                                                  unknown                 M          Mito                               0
                                                                                                                complex); may promote binding of incoming p
YPL078C     ATP4      Subunit b of the stator stalk of mitochondrial F1F0protonsynthase, which Mitolarge, evolutionarily conserved enzyme complex req
                                             735 ATP synthesis structural molecule activity
                                                                   coupled ATP transport  M          is a       ATP4                    0
YPL099C     FMP14                            549 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP14 purified mitochondria in high-through
                                                                    authentic,            M          Mito       in highly               1
YPL104W     MSD1                            1977 protein biosynthesis
                                                                   required for acylationM  activity Mito       MSD1                    0
                      Mitochondrial aspartyl-tRNA synthetase, aspartate-tRNA ligaseof aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and l
YPL135W     ISU1      Conserved protein of 498 mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts p
                                             the iron ion homeostasis binding
                                                                  protein                 M          Mito       ISU1                    0
YPL155C     KIP2      Kinesin-related motor protein involved in microtubule motor activity stabilizes microtubules by targeting Bik1p to the plus end; Ki
                                            2121 nuclear migration, microtubule-mediated
                                                                   mitotic spindle positioning,
                                                                                          M          Mito       KIP2                    0
YPL188W     POS5                             kinase, phosphorylates NADH; also phosphorylatesMito
                                                                  NADH stress
                      Mitochondrial NADH1245 response to oxidative kinase activityM                             POS5                    0
                                                                                                       NAD(+) with lower specificity; required for the response
YPL270W     MDL2      Half-type ATP-binding cassette (ABC) transporteractivity,inner M to transmembrane movement of substances
                                            2322 aerobic respiration
                                                                  ATPase of the coupled              Mito
                                                                                          mitochondrial membraneMDL2                    3
YPR001W     CIT3      Citrate synthase, catalyzes the condensation of(Si)-synthase activity
                                            1461 tricarboxylic acid cycleacetyl coenzyme A and oxaloacetate to form citrate, mitochondrial isozyme invol
                                                                  citrate                 M          Mito       CIT3                    0
YPR004C     YPR004C                         1035 unknown
                      Electron transfer flavoprotein complex subunit ETF-alpha; contains a FAD binding domain; interacts with 0
                                                                  electron carrier activity
                                                                                          M          Mito       YPR004C                 YFH1, the yeast frataxin
YPR024W     YME1      Subunit, with Mgr1p, of the mitochondrialATP-dependent peptidase activity complex, which is responsible 0 degradation of unfol
                                            2244 mitochondrial organization and biogenesis
                                                                    inner membrane i-AAA protease
                                                                                          M          Mito       YME1                    for
YPR058W     YMC1                             924 transport        transporter member M               Mito       YMC1                    0
                      Putative mitochondrial inner membrane transporter, activity of the mitochondrial carrier (MCF) family; the authentic, non-tagged
YPR067W     ISA2                             558 iron of transport
                      Protein required for maturationion mitochondrial and cytosolic Fe/S proteins, localizes to the mitochondrial intermembrane space
                                                                  unknown                 M          Mito       ISA2                    0
YPR095C     SYT1      Guanine nucleotide 3681 vesicle-mediated forguanyl-nucleotide exchange factor transport; suppressor of 0
                                            exchange factor (GEF) transport
                                                                  ARF Arf proteins; involved in vesicular activity
                                                                                          M          Mito       SYT1                    ypt3 mutations; membe
YPR125W     YPR125W                         1365 unknown          unknown                 M          Mito       YPR125W
                      Mitochondrial inner membrane protein exposed to the mitochondrial matrix, associates with mitochondrial1ribosomes, NOT requir
YPR133W-A   TOM5      Small mitochondrial outer mitochondral protein import a binding relay for the import of proteins into mitochondria; subunit on the
                                             153 membrane protein crucial to
                                                                  protein transporter activity
                                                                                          M          Mito       TOM5                    1
YPR191W     QCR2                            1107 cytochrome-c reductase complex, which is a component QCR2mitochondrial inner membrane electron
                                                                  ubiquinol-cytochrome-c reductase activity of the
                      Subunit 2 of the ubiquinol aerobic respiration                      M          Mito                               0
YKR019C     IRS4      Protein involved in regulation of phosphatidylinositolbiogenesis A
                                            1848 cell wall organization and 4,5-bisphosphate concentrations; Irs4p and Tax4p bind and activate the pho
                                                                  unknown                 M,         Mito       IRS4                    0
YBL057C     PTH2                             PTH1) mitochondrially-localized peptidyl-tRNA activity
                                                                  aminoacyl-tRNA hydrolase hydrolases; negatively regulates the 1
                      One of two (see also 645 protein biosynthesis                       M, C       cytoplasm PTH2                     ubiquitin-proteasome pa
YBR121C     GRS1                            2004 glycyl-tRNA aminoacylation ligase activity
                                                                  glycine-tRNA
                      Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates C               to the cognate anticodon bearing tRNA; transcription ter
                                                                                          M, glycine cytoplasm GRS1                     0
YBR129C     OPY1                             987 conjugation with cellular fusion
                                                                  unknown                 M, C       cytoplasm of mating pheromone; the authentic, non-tag
                      Protein of unknown function, overproduction blocks cell cycle arrest in the presenceOPY1                          0
YCL057W     PRD1                            2139 proteolysis      cytoplasm and intermembrane cytoplasm PRD1
                                                                                          M, C                                          0
                      Zinc metalloendopeptidase, found in the metalloendopeptidase activity space of mitochondria; with Cym1p, involved in degradat
YDL230W     PTP1                            1008          amino acid dephosphorylation C
                                                                  protein tyrosine phosphatase activity
                                                                                          M,         cytoplasm PTP1
                      Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo,0including Fpr3p; localiz
YDL240W     LRG1                            3054 small GTPase Rho GTPasethe Pkc1p-mediated signaling pathway that controls cell wall integrity; appe
                                                                   mediated in activator activity
                                                                                          M, C
                      Putative GTPase-activating protein (GAP) involved signal transduction cytoplasm LRG1                              0
YDR226W     ADK1                             669 nucleotide metabolism; kinase activity cytoplasm and the mitochondria; lacks 0
                                                                  adenylate
                      Adenylate kinase, required for purinemetabolism localized to M, C   the        cytoplasm ADK1                     cleavable signal seque
YDR341C     YDR341C                         1824 synthetase arginine-tRNA ligase M, C
                      Cytoplasmic arginyl-tRNA protein biosynthesis                       activity   cytoplasm YDR341C                  0
YDR505C     PSP1                            2526 unknown          unknown                 M, C       cytoplasm polymerase alpha) and partial suppressor of C
                      Asn and gln rich protein ofunknown function; high-copy suppressor of POL1 (DNA PSP1                               0
YDR513W     GRX2      Cytoplasmic glutaredoxin, response to oxidative stress exchange intermediate activity
                                             432 thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of
                                                                  thiol-disulfide         M, C       Mito       GRX2                    1
YFR011C     YFR011C                          513 unknown            authentic,            M, C       cytoplasm in highly purified mitochondria in high-through
                      Putative protein of unknown function; theunknown non-tagged protein is detected YFR011C                           0
YFR015C     GSY1                            2127 glycogen Gsy2p, the (starch) synthase activity
                                                                  glycogen                M, C       cytoplasm GSY1                     0
                      Glycogen synthase with similarity tobiosynthesismore highly expressed yeast homolog; expression induced by glucose limitation,
YGL221C     NIF3                             867 unknown          unknown                 M, C       cytoplasm NIF3                     0
                      Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagge
YGR094W     VAS1                            3315 valyl-tRNA aminoacylation
                                                                  valine-tRNA
                      Mitochondrial and cytoplasmic valyl-tRNA synthetase ligase activity M, C       cytoplasm VAS1                     0
YGR155W     CYS4                            1524 cysteine biosynthesis
                                                                  cystathionine beta-synthase from serine and homocysteine, the0
                                                                                          M, C
                      Cystathionine beta-synthase, catalyzes the synthesis of cystathionineactivity  cytoplasm CYS4                      first committed step in
YGR240C     PFK1                            2964 glycolysis       6-phosphofructokinase activity cytoplasm PFK1
                                                                                          M, C
                      Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic0growth, activated by fru
YIL078W     THS1                            2205 protein biosynthesis
                                                                  threonine-tRNA          M, C
                      Threonyl-tRNA synthetase, essential cytoplasmic proteinligase activity cytoplasm THS1                             0
YIL094C     LYS12                           1116 lysine an NAD-linked mitochondrialM, C
                      Homo-isocitrate dehydrogenase,biosynthesis                          enzyme activity
                                                                                                     cytoplasm the fourth step in the biosynthesis of lysine, in
                                                                  homoisocitrate dehydrogenaserequired for LYS12                        0
YJL060W     BNA3                            1335 NAD biosynthesis arylformamidase from tryptophan via kynurenine pathway; potential Cdc28p substrate
                                                                                          M,
                      Arylformamidase, involved in biosynthesis of nicotinic acidactivity C          cytoplasm BNA3                     0
YJL098W     SAP185                          complex with the Sit4p protein phosphatase phosphatase activity function; member of a family of similar pro
                                                                    of mitotic cell cycle M, C       cytoplasm its
                      Protein that forms a 3177 G1/S transitionprotein serine/threonine and is required forSAP185                       0
YJL130C     URA2                            6645 'de novo' pyrimidine base biosynthesis transcarbamylase (ATCase), catalyzes0 first two enzymatic
                                                                  aspartate carbamoyltransferasecytoplasm URA2
                      Bifunctional carbamoylphosphate synthetase (CPSase)-aspartateC      M,          activity                           the
YJR098C     YJR098C                         1968 unknown            authentic,            M, C       cytoplasm in highly purified mitochondria in high-through
                      Putative protein of unknown function; theunknown non-tagged protein is detected YJR098C                           0
YKL010C     UFD4                            4452 protein monoubiquitination
                                                                  ubiquitin-protein Rpt6p, two subunits of the 19S
                                                                                          M, C                                          0
                      Ubiquitin-protein ligase (E3) that interacts with Rpt4p and ligase activity cytoplasm UFD4 particle of the 26S proteasome; cytopla
YKL067W     YNK1      Nucleoside diphosphate kinase, catalyzes the transfer of gamma kinase activity
                                             462 purine nucleotide biosynthesis
                                                                  nucleoside diphosphate C           cytosol    YNK1                    0
                                                                                          M, phosphates from nucleoside triphosphates, usually ATP, to nuc
YKL152C     GPM1                             744 glycolysis
                      Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis a
                                                                  phosphoglycerate mutase activity
                                                                                          M, C       cytosol    GPM1                    0
YKL157W     APE2                            3191 peptide yscII, may have a role in obtaining leucine from dipeptide
                                                                  leucyl                  M, C                                          0
                      Zinc-dependent metallopeptidase metabolism aminopeptidase activity cytoplasm APE2 substrates; sequence coordinates ha
YLR351C     NIT3                             876 unknown          hydrolase similarity to the carbon-nitrogen (but not fly and worm and
                                                                                          M, on      cytoplasm NIT3                     0
                      Nit protein, one of two proteins in S. cerevisiae withactivity, actingC Nit domain of NitFhit from peptide) bonds to the mouse an
YLR419W     YLR419W                         4308 unknown          helicase activity       M, C       cytoplasm YLR419W                  is
                      Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein 0 detected in highly pu
YMR031C     YMR031C                         2532 unknown            similarity            M, C       cytoplasm YMR031C                  0
                      Putative protein of unknown function withunknown to YKL050C and USO1/YDL058W; the authentic, non-tagged protein is detec
YMR083W     ADH3                            1128 fermentation alcohol dehydrogenase C     M, shuttling of       ADH3                    0
                      Mitochondrial alcohol dehydrogenase isozyme III; involved in theactivity Mito mitochondrial NADH to the cytosol under anaerobic
YMR089C     YTA12                           2478 protein complex assembly
                                                                  ATPase activity
                      Component, with Afg3p, of the mitochondrial inner membrane M, C               cytoplasm mediates degradation of misfolded or unasse
                                                                                         m-AAA protease thatYTA12                     0
YNL104C     LEU4                            1860 leucine biosynthesis
                                                                  2-isopropylmalate synthase activity
                                                                                          the       cytoplasm LEU4                    0
                      Alpha-isopropylmalate synthase (2-isopropylmalate synthase);M, Cmain isozyme responsible for the first step in the leucine biosy
YNL256W     FOL1                            2475 the acid and derivative biosynthesishas
                                                                  2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity
                                                                                                    cytoplasm FOL1                    0
                      Multifunctional enzyme of folicfolic acid biosynthesis pathway, M, Cdihydropteroate synthetase, dihydro-6-hydroxymethylpterin py
YOL045W     PSK2                            3306 protein domain containing S/T protein kinase activity
                                                                  protein serine/threonine C          regulates sugar                 0
                      One of two (see also PSK1) PAS amino acid phosphorylation M,kinases;cytoplasm PSK2 flux and translation in response to an u
YOL059W     GPD2      NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an 0
                                            1323 glycerol metabolism
                                                                  glycerol-3-phosphate M, C
                                                                                         dehydrogenase (NAD+) activity
                                                                                                    Mito         GPD2                   oxygen-independent sig
YOR251C     YOR251C                          915 unknown                      to cyanideM, C
                                                                                          to form sulfite
                                                                                                    cytoplasm YOR251C
                      catalyzes transfer of the sulfane atom of thiosulfate sulfurtransferase activity and thiocyanate                0
YOR335C     ALA1                            2877 alanyl-tRNA aminoacylation ligase activity
                                                                  alanine-tRNA           M, C       cytoplasm (cdc64-1 allele) causes cell cycle arrest at G1
                      Cytoplasmic alanyl-tRNA synthetase, required for protein synthesis; point mutation ALA1                         0
YPL226W     NEW1                            3591 unknown          ATPase activity
                      ATP binding cassette family member; Asn/Gln-rich rich regionM, C              cytoplasm formation, susceptibility to [PSI+] prion induc
                                                                                          supports [NU+] prionNEW1                    0
YPL231W     FAS2                            5664 synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl r
                                                                  phosphopantetheinyltransferaseMito
                      Alpha subunit of fatty acidfatty acid biosynthesis                 M, C         activity FAS2                   0
YPL262W     FUM1      Fumarase, converts1467 tricarboxylic acid cycle inhydratasecycle; cytosolic and mitochondrial localization determined by the N-te
                                            fumaric acid to L-malic acid the TCA activity
                                                                  fumarate               M, C       cytosol      FUM1                 0
YPR033C     HTS1                            1641 histidyl-tRNA tRNA synthetase; encoded
                                                                  histidine-tRNA         activity    single nuclear gene that specifies two messages; efficie
                      Cytoplasmic and mitochondrial histidineaminoacylation ligase M, C by a cytoplasm HTS1                           0
YAL035W     FUN12                           3009 translational initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining
                                                                  initiation
                      GTPase, required for general translation GTPase activity           M, C, R Mito            FUN12                0
YDL040C     NAT1                            2565 protein amino acid acetylation Ard1p, Nat5p); cytoplasm acetylates many proteins, which influences
                                                                  peptide (Nat1p,        M, C, R N-terminally
                      Subunit of the N-terminal acetyltransferase NatA alpha-N-acetyltransferase activity NAT1                        0
YGL011C     SCL1                             759 ubiquitin-dependent protein catabolism
                                                                  endopeptidase activityinvolved Mito            SCL1                 0
                      Alpha subunit of the 20S core complex of the 26S proteasomeM, C, R in the degradation of ubiquitinated substrates; essential f
YGL195W     GCN1                            the Gcn2p kinase translational elongationM, C, Gcn20p;
                                                                  unknown                                        GCN1                 0
                      Positive regulator of 8019 regulation of activity, forms a complex withR Mito proposed to stimulate Gcn2p activation by an unch
YKR094C     RPL40B                           755 Rpl40Ap, thatstructural constituent M, ribosome ribosomal protein of the large 0
                                                                   is cleaved to yield ubiquitinR Mito
                      Fusion protein, identical toprotein biosynthesis                   of C, and a             RPL40B                (60S) ribosomal subuni
YML026C     RPS18B                            the protein biosynthesis
                                                                  structural constituent M, ribosome Rps18Ap and has similarity to0E. coli S13 and rat S18
                                                                                         of C, R to
                      Protein component of842 small (40S) ribosomal subunit; nearly identical cytosol            RPS18B
YLL040C     VPS13                           9435 sporulation
                      Protein of unknown function; heterooligomeric or homooligomericD
                                                                  unknown                           Mito         VPS13                0
                                                                                         M, complex; peripherally associated with membranes; homologou
YOR089C     VPS21                            633 endocytosis GTPase and for
                      GTPase required for transport during endocytosis activity correct D            of vacuolar hydrolases; localized in endocytic intermedia
                                                                                                                 VPS21
                                                                                         M, sorting endocytic intermediate, Mito      0
YNR016C     ACC1                            6702 protein-nucleus enzyme carboxylase E carboxylation of acetyl-CoA to form malonyl-CoA; required f
                                                                  acetyl-CoA             M, the
                      Acetyl-CoA carboxylase, biotin containing import that catalyzes activity Mito              ACC1                 0
YMR264W     CUE1                             612 ER-associatedprotein binding
                                                                   protein catabolism M, E          Mito         CUE1
                      Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER0where it functions in pr
YLR100W     ERG27                           1044 ergosterol biosynthesis
                                                                  3-keto sterol reductase activity ER
                                                                                         M, to                   ERG27                0
                      3-keto sterol reductase, catalyzes the last of three steps required E remove two C-4 methyl groups from an intermediate in ergos
YHR190W     ERG9      Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl activity
                                            1335 ergosterol biosynthesis
                                                                  farnesyl-diphosphate M, E
                                                                                         farnesyltransferase pyrophosphate moieties to form squalene in th
                                                                                                    ER           ERG9                 1
YGL020C     MDM39     Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion
                                             708 mitochondrial organization and biogenesis
                                                                  unknown                M, E       ER           MDM39                0
YPR113W     PIS1                             663 cell required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, s
                      Phosphatidylinositol synthase,cycle                                M, E       ER           PIS1
                                                                  CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity 3
YBR229C     ROT2      Glucosidase II catalytic subunit required alpha-glucosidase activity
                                            2865 cell wall biosynthesis
                                                                  for normal cell wall synthesis; mutations inROT2
                                                                                         M, E       Mito                              0
                                                                                                                  rot2 suppress tor2 mutations, and are synthe
YOR254C     SEC63                           Sec63 complex (Sec63p, Sec62p, Sec66p and
                                                                  protein transporter activity
                                                                                         targeting Mito          SEC63                3
                      Essential subunit of 1992 posttranslational protein-membrane M, E Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a
YML013W     SEL1      Protein involved in ER-associatedsecretion
                                            1755 protein protein degradation; proposed E coordinate the assembly of proteins involved in ERAD; conta
                                                                  unknown                M, to      ER           SEL1                 0
YDL015C     TSC13     Enoyl reductase that catalyzes the last step in each metabolism E
                                             933 very-long-chain fatty acid cycle of very long chain fatty acid elongation, localizes to the ER, highly enric
                                                                  oxidoreductase activityM,         Mito         TSC13                4
YOR285W     YOR285W                          420 unknown
                      Protein of unknown function, localized to unknown
                                                                   the mitochondrial outer membrane
                                                                                         M, E       Mito         YOR285W              0
YML048W     GSF2                            1212 protein folding unknown                 M, E, of cytoplasm GSF2                      1
                      ER localized integral membrane protein that may promote secretion C certain hexose transporters, including Gal2p; involved in g
YBR265W     TSC10                            963 sphingolipid biosynthesis
                                                                  oxidoreductase in phytosphingosine synthesis, essential for growth in the absence of ex
                                                                                         M, E, C cytoplasm TSC10
                      3-ketosphinganine reductase, catalyzes the second step activity, acting on NADH or NADPH                        1
YDR027C     VPS54     Component of the GARP (Golgi-associatedtransport protein) complex, Mito
                                            2670 Golgi to vacuole retrograde
                                                                  unknown                M, G                    VPS54                0
                                                                                                     Vps51p-Vps52p-Vps53p-Vps54p, which is required for t
YGL225W     VRG4      Golgi GDP-mannose transporter; amino acid N-linked glycosylation activityin Golgi
                                            1014 protein regulates Golgi function transporter
                                                                  nucleotide-sugar and M, G
                                                                                         glycosylation
                                                                                                    Mito         VRG4                 9
YJL178C     ATG27                            816 that binds phosphatidylinositol 3-phosphate, required for ATG27
                                                                  protein kinase regulator G, A
                      Type II membrane proteinvesicle organization and biogenesis M, activity autophagosome?                          1
                                                                                                                  the cytoplasm-to-vacuole targeting (Cvt) path
YBR097W     VPS15                           4365 protein amino acid involved in vacuolarG, C Mito
                                                                  protein serine/threonine protein sorting; VPS15                     0
                      Myristoylated serine/threonine protein kinase phosphorylation M, kinase activity functions as a membrane-associated complex
YDR233C     RTN1                             888 unknown           exocyst               M, G, with Yip3p; null mutant has an altered (mostly cisternal) ER
                      ER membrane protein that interacts with unknownsubunit Sec6p and E Mito                    RTN1                 2
YJR089W     BIR1      Essential chromosomal passenger protein involved in coordinating cell cycle events for proper chromosome segregation; C-term
                                            2865 chromosome segregation
                                                                  unknown                M, N       Mito         BIR1                 0
YJR060W     CBF1                            1056 chromatin assemblybinding
                                                                  DNA or disassembly present atnucleus sites including MET gene promoters and centro
                      Helix-loop-helix protein that binds the motif CACRTG, which isM, N              several CBF1                    0
YPR025C     CCL1                            1182 transcription initiation from RNA TFIIH-associated carboxy-terminal domain (CTD) kinase involved in tr
                                                                  general RNA the polymerase Mito
                                                                                         M, N        promoter CCL1
                      Cyclin associated with protein kinase Kin28p, which is polymerase II TFIIactivity                               0
YDR301W     CFT1      RNA-binding subunit of the mRNA cleavage and polyadenylation N
                                            4074 mRNA polyadenylylation
                                                                  RNA binding                       Mito          poly(A)             0
                                                                                         M, factor; involved in CFT1 site recognition and required for both
YER164W     CHD1      Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase dom
                                            4407 chromatin remodeling activity
                                                                  ATPase                 M, N       Mito         CHD1                 0
YEL061C     CIN8                            3003 mitotic mitotic spindle assembly and chromosome segregation
                                                                  microtubule motor activity
                      Kinesin motor protein involved in sister chromatid segregation M, N           Mito         CIN8                 0
YMR078C     CTF18                           2226 Ctf8p sister chromatid subunits M, N
                                                                  unknown                           Mito         CTF18                0
                      Subunit of a complex with mitoticthat shares somecohesion with Replication Factor C and is required for sister chromatid cohesio
YOL021C     DIS3                            3006 35S primary transcript processing and RNA degradation, binds Gsp1p/Ran and enhances the GEF ac
                                                                   in rRNA processing activity Mito
                      Nucleolar exosome component, involved3'-5'-exoribonuclease M, N                            DIS3                 0
YAL019W     FUN30                           3396 chromosome organization and biogenesis
                                                                  unknown                M, N       nucleus FUN30                     0
                      Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p; the authentic, non-ta
YDR096W     GIS1                            2685 spore wall expression
                                                                  TF activity
                      Transcriptional factor, involved in theassembly of genes during N               limitation; also                0
                                                                                         M, nutrientnucleus GIS1 involved in the negative regulation of DP
YDL207W     GLE1                            1617 poly(A)+ mRNA-nucleus export
                                                                  unknown                M, but      for           import;
                      Cytoplasmic nucleoporin required for polyadenylated RNA export N notMitoproteinGLE1 component of0Nup82p nuclear pore s
YOL089C     HAL9                            3093 transcription a specific RNA polymerase II TF activity
                                                                  zinc finger; RNA polymerase II nucleus HAL9
                                                                                         M, N        promoter                         0
                      Putative transcription factor containing initiation from overexpression increases salt tolerance through increased expression of the
YGL073W     HSF1                            transcription factor, activates multiple genes in
                                                                  TF activity             polymerase II to stresses
                                                                                                    nucleus HSF1                      0
                      Trimeric heat shock 2502 regulation of transcription from RNAM, N responsepromoter that include hyperthermia; recognizes v
YKR063C     LAS1                            1509 establishment of cell polarity
                                                                  unknown                M, N       nucleus LAS1                      0
                      Essential nuclear protein possibly involved in bud formation and morphogenesis; mutants require the SSD1-v allele for viability
YKL205W     LOS1                             involved in nuclear tRNA of pre-tRNA M, N
                      Nuclear pore protein3303 tRNA-nucleus export binding                          Mito         LOS1                 0
YDL182W     LYS20                           1287 lysine biosynthesis condensation ofM, N
                                                                  homocitrate synthase acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is
                      Homocitrate synthase isozyme, catalyzes the via aminoadipic acid    activity nucleus LYS20                      0
YOR298C-A   MBF1                             456 that bridges the DNA-binding region ofactivity nucleus MBF1 protein Spt15p; suppressor of frame
                                                                  transcription coactivator RNA polymerase II promoter
                                                                                         M, Gcn4p and TATA-binding
                      Transcriptional coactivatorpositive regulation of transcription from N                                          0
YLR106C     MDN1                          14733 rRNA processing   ATPase forms           M, N       nucleus with the
                      Huge dynein-related AAA-type ATPase (midasin),activity extended pre-60S particle MDN1 Rix1 complex0(Rix1p-Ipi1p-Ipi3p), ma
YBR136W     MEC1                            7107 meiotic recombination superfamily member;nucleus MEC1 required for cell cycle arrest and trans
                                                                   PI kinase
                      Genome integrity checkpoint protein andprotein kinase activityM, N             signal transducer                0
YDR296W     MHR1                             681 mitochondrial genome mitochondria and
                                                                  transcription regulator activity nucleus MHR1                       0
                      Protein involved in homologous recombination in maintenance M, N in transcription regulation in nucleus; binds to activation doma
YMR167W     MLH1                            2310 meiotic recombination meiosis, postmeiotic nucleus MLH1 spore viability; 0
                                                                  DNA and
                      Protein required for mismatch repair in mitosisbinding             M, N       segregation, and                   forms a complex with P
YPL082C     MOT1      Essential abundant protein involved in regulation of transcription, N
                                            5604 regulation of transcription from RNAM, removes Spt15p (TBP) from DNA via its C-terminal ATPase ac
                                                                  ATPase activity                   Mito
                                                                                          polymerase II promoter MOT1                 0
YMR224C   MRE11                           2079 Rad50p and protein binding             M, N         nucleus MRE11                      0
                    Subunit of a complex with DNA repair Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telome
YCL017C   NFS1                            1494 iron in homeostasis
                                                               cystathionine gaMMa-lyase activity
                                                                                      M, N         Mito        NFS1
                    Cysteine desulfurase involved ioniron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional0thio-modification of mit
YGR159C   NSR1                            1245 rRNA processing binding
                                                               RNA                    M, N         nucleus NSR1                       0
                    Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
YAL015C   NTG1                            and apurinic/apyrimidinic (AP) lyase involved N basenucleus activity localizes to the 0
                    DNA N-glycosylase 1200 DNA repair                                 M, in         excision NTG1
                                                               DNA-(apurinic or apyrimidinic site) lyase repair,                      nucleus and mitochond
YJL208C   NUC1                             990 DNA recombination
                                                               ribonuclease activity*M, N          Mito        NUC1                   1
                    Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has a role in mitochondrial recombinatio
YLR335W   NUP2                            2163 mRNA-nucleus export molecule activity
                                                               structural             M, N         Mito        NUP2
                    Protein involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of0the nuclear pore comp
YGL151W   NUT1                            3399 regulation of transcription from which is required II promoter
                                                               unknown
                    Component of the RNA polymerase II mediator complex, RNAM, N                   nucleus NUT1                       0
                                                                                       polymerase for transcriptional activation and also has a role in bas
YOR386W   PHR1                            1698 photoreactive repair
                                                               deoxyribodipyrimidineM, dimers nucleus PHR1of visible light; induced by DNA damage
                    DNA photolyase involved in photoreactivation, repairs pyrimidine N             in activity
                                                                                        photo-lyase the presence                      0
YML061C   PIF1                            2580 mitochondrial genome elongation; acts as
                                                               DNA helicase activityM,             nucleus PIF1                       0
                    DNA helicase involved in telomere formation andmaintenance N a catalytic inhibitor of telomerase; also plays a role in repair
YNL102W   POL1                            4407 DNA replicationalpha-primase complex,N
                                                                  initiation          M, required for the initiation
                                                                                                               fork                   0
                    Catalytic subunit of the DNA polymerasealpha DNA polymerase activity replication POL1 of DNA replication during mitotic DNA
YMR129W   POM152    Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phas
                                          4014 mRNA-nucleus export molecule activity
                                                               structural             M, N         Mito        POM152                 3
YEL037C   RAD23                           1197 ER-associateddamaged DNA binding , N
                                                                protein and binds M                Mito        RAD23                  0
                    Protein with ubiquitin-like N terminus, recognizes catabolism damaged DNA (with Rad4p) during nucleotide excision repair; regula
YNL250W   RAD50                           3939 with Mre11p and Xrs2p, involved in processingnucleus RAD50 breaks in vegetative cells, initiation
                                                               protein binding
                    Subunit of MRX complex, double-strand break repair                M, N          double-strand DNA                 0
YOR048C   RAT1                            3021 35S RNA exonuclease, involved RNA
                                                               5'-3' exoribonuclease activity nucleus RAT1                            0
                    Nuclear 5' to 3' single-strandedprimary transcript processing in M, N metabolism, including rRNA and snRNA processing as well a
YOR191W   RIS1                            4860 chromatin assembly or disassemblyM, N
                                                               DNA-dependent ATPase activity       nucleus RIS1                       0
                    Member of the SWI/SNF family of DNA-dependent ATPases, plays a role in antagonizing silencing during mating-type switching,
YOR151C   RPB2                            3675 transcription from RNA polymerase II core; similar to bacterial beta subunit
                                                               DNA-directed RNA polymerase activity
                                                                                      M, N                     RPB2
                    RNA polymerase II second largest subunit B150, part of central promoterDNA-directed RNA Pol II, core complex      0
YDL140C   RPO21                           5202 subunit B220,DNA-directed RNA polymerase Mito
                                                                 part of polymerase II promoteractivity
                                                                                      M, N                     RPO21
                    RNA polymerase II largesttranscription from RNAcentral core; phosphorylation of C-terminal heptapeptide0            repeat domain regulate
YLR442C   SIR3      Silencing protein that interacts with Sir2phistone binding histone H3 and Mito
                                          2937 double-strand breakSir4p, and
                                                                and repair            M, N                      establish a transcriptionally silent chromatin
                                                                                                   H4 tails, toSIR3                   0
YFR031C   SMC2                            3513 mitotic sister chromatid activity
                                                               ATPase segregation M, N
                    Component of the condensin complex, essential SMC chromosomal ATPase familySMC2Mito         member that forms a0complex with Smc4p to
YLR383W   SMC6                            3345 DNA repair      unknown                M, N         nucleus SMC6                       0
                    Protein involved in structural maintenance of chromosomes; essential subunit of Mms21-Smc5-Smc6 complex; required for grow
YGL093W   SPC105    Protein required for accurate chromosome segregation, localizescytoskeleton side of the spindle pole body; forms a complex w
                                          2754 microtubule nucleation constituent M, N the nuclear
                                                               structural             of to        Mito        SPC105                 0
YML010W   SPT5                            complex with Spt4p RNAmediates both activation nucleus
                                                               and polymerase II transcription elongation of transcription
                                                                                      M, N                      factor                0
                    Protein that forms a 3192 regulation of transcription, DNA-dependent and inhibitionSPT5 activity elongation; Spt4p-Spt5p com
YGR116W   SPT6                            4356 regulation of transcription, DNA-dependent
                                                               RNA polymerase II transcriptionnucleus SPT6and reassembly 0
                                                                                       nucleosome disassembly activity
                    Essential protein that interacts with histones and is involved inM, N           elongation factor                  during transcription elo
YBR081C   SPT7      Subunit of the SAGA transcriptional regulatory complex, involved N proper assembly of the complex; also present as a C-termina
                                          3999 protein complex assembly
                                                               structural molecule activity
                                                                                      M, in        Mito        SPT7                   0
YAL011W   SWC3                            1878 chromatin remodelingSWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bo
                                                                of the
                    Protein of unknown function, component unknown                    M, N         nucleus SWC3                       0
YKL028W   TFA1                            involved in recruitment of RNA polymerase IINII the promoter, activation
                                                               general RNA polymeraseto TF TF TFIIE TFA1
                                                                                      M,           promoter                           1
                    TFIIE large subunit, 1449 transcription initiation from RNA polymerase IIactivity complex of TFIIH, and promoter opening
YAL001C   TFC3                            3573 transcription initiation from RNA III TF N
                                                               RNA polymerase polymerase IIIMito
                                                                                       initiation promoter TFC3                       0
                    Largest of six subunits of the RNA polymerase III transcriptionM,activity factor complex (TFIIIC); part of the TauB domain of TFIII
YKR010C   TOF2      Nonessential mitochondrial protein of unknown function with sequence similarity to TOF2 identified as a 0
                                          2316 DNA topological change
                                                               unknown                M, N         Mito        Net1p;                 topoisomerase I (Top1p
YNL088W   TOP2                            4287 meiotic recombination strain in DNA by cleaving and re-sealing the phosphodiester backbone of both
                                                                torsional             M, N         nucleus TOP2
                    Essential type II topoisomerase, relieves DNA topoisomerase (ATP-hydrolyzing) activity                            0
YML076C   WAR1                            2835 response to acid activity
                                                               TF                     M, N         nucleus WAR1                       0
                    Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 a
YLR090W   XDJ1                             homolog of E.       chaperone regulator activity
                                                                                      M, N         nucleus XDJ1                       0
                    Putative chaperone,1380 unknown coli DnaJ, closely related to Ydj1p; the authentic, non-tagged protein is detected in highly pur
YLR247C   YLR247C                         4671 unknown         helicase activity      M, N         nucleus YLR247C                    0
                    Putative helicase; localized to mitochondria and the nucleus; YLR247C is not an essential gene; null mutant displays increased l
YDR035W   ARO3                            1113 aromatic amino acid (DAHP) synthase,N, C synthase activity
                                                               3-deoxy-7-phosphoheptulonate cytoplasm ARO3
                                                                                      M,                                              0
                    3-deoxy-D-arabino-heptulosonate-7-phosphate family biosynthesis catalyzes the first step in aromatic amino acid biosynthesis an
YNL112W   DBP2                            2643 RNA helicase of thehelicase activityM, family, involved in nonsense-mediated mRNA decay and rRNA
                                                               RNA DEAD-box protein decay
                    Essential ATP-dependent mRNA catabolism, nonsense-mediatedN, C cytoplasm DBP2                                     0
YOR144C   ELG1                            2376 DNA replicationand telomere homeostasis, forms an alternative replication factor C complex important
                    Protein required for S phase progressionunknown                   M, N, C cytoplasm ELG1                          0
YBL016W   FUS3                            1062 protein amino acid phosphorylation M, N, C cytoplasm FUS3
                                                               MAP kinase activity                                                    0
                    Mitogen-activated protein kinase involved in mating pheromone response; activated by phosphorylation by Ste7p; provides speci
YPL091W   GLR1                            1452 response to oxidative stress
                                                               glutathione-disulfide reductase cytoplasm GLR1reduced glutathione
                                                                                      M, oxidized glutathione to
                    Cytosolic and mitochondrial glutathione oxidoreductase, convertsN, C activity                                     0
YJL082W   IML2                            2196 unknown         unknown                  is detected in highly purified mitochondria 0 high-throughput stud
                    Protein of unknown function; the authentic, non-tagged proteinM, N, C cytoplasm IML2                               in
YHR179W   OYE2                            1203 unknown         NADPH dehydrogenase N, C cytoplasm homologous to Oye3p with slight differences
                                                                                      M, activity
                    Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN),OYE2                                 0
YBR150C   TBS1                            3285 unknown          authentic,            M, N, C cytoplasm in highly                     0
                    Putative protein of unknown function; theunknown non-tagged protein is detected TBS1 purified mitochondria in high-through
YFL010C   WWM1                             636 response to desiccation
                                                               unknown                M, N, C cytoplasm WWM1                          0
                    WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced a
YNL274C   YNL274C                         1053 mutation results in increased activity, after diauxic CH-OH group
                                                                                      M, N, C cytoplasm the authentic, non-tagged protein is detected
                                                                                                                                      0
                    Glyoxylate reductase; null metabolism oxidoreductasebiomass acting on the shift; YNL274Cof donors, NAD or NADP as accept
YIL016W   SNL1                             480 protein folding be involved in nuclear pore complex
                                                                                      M, N,                    SNL1                   1
                    Protein of unknown function proposed to chaperone regulator activityE Mito biogenesis and maintenance as well as protein fold
YJR117W   STE24                           1362 peptide pheromone maturation
                                                               metalloendopeptidase activity
                                                                                      M, of a-factor           STE24                  4
                    Highly conserved zinc metalloprotease that functions in two stepsN, E ER maturation, C-terminal CAAX proteolysis and the fir
YDR196C   YDR196C                          726 coenzyme A biosynthesis
                                                                that catalyzes the final activity ER
                                                                                      M, N, E
                    Putative dephospho-CoA kinase (DPCK)dephospho-CoA kinase step in Coenzyme YDR196C                                 0
                                                                                                               A biosynthesis; essential for viability; the auth
YMR128W   ECM16                           3804 processing of 20S pre-rRNA activityM, N, R Mito
                                                               RNA helicase                                    ECM16                  0
                    Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required f
YIL149C   MLP2                            5040 mRNA-nucleus export
                                                               ribonucleoprotein binding R Mito
                                                                                      M, N,                    MLP2                   0
                    Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in
YOR206W   NOC2                            nucleolar complex with Mak21p that bindsM, N, R and 66S pre-ribosomes, as well as0 nuclear complex with
                    Protein that forms a 2133 ribosome assemblyunknown                  to 90S nucleus NOC2                             a
YDL148C   NOP14                           2433 complex with Noc4p that mediates maturationMito nuclear export of 40S ribosomal subunits; also pres
                                                               snoRNA binding
                    Nucleolar protein, forms aprocessing of 20S pre-rRNA              M, N, R and              NOP14                  0
YGR091W   PRP31                           1485 nuclear mRNARNA splicing spliceosome transesterification mechanism
                                                                splicing, via factor M, N,
                    Splicing factor, component of the U4/U6-U5 snRNP complexactivity,R Mito                    PRP31                  0
YGL120C   PRP43                           DEAH-box family, functions in both RNA polymerase I and
                                                               RNA splicing factor M, N, transesterification mechanism
                                                                                                               PRP43                  0
                    RNA helicase in the 2304 U2-type spliceosome disassembly activity,R Mito polymerase II transcript metabolism, involved in r
YML091C   RPM2                            3609 protein biosynthesis
                                                               ribonuclease P activity , N, R Mito
                                                                                      M                        RPM2                   0
                    Protein component of mitochondrial RNase P, along with the mitochondrially-encoded RNA subunit RPM1; Rnase P removes 5'
YJL109C   UTP10                           5310 processing of 20S pre-rRNA
                                                               snoRNA (SSU)           M, N, R Mito             UTP10                  0
                    Nucleolar protein, component of the small subunitbinding processome containing the U3 snoRNA that is involved in processing o
YPL217C   BMS1                             nucleolar GTP-binding binding
                                                               GTP                    M, N, R, Ccytoplasm BMS1                        for
                    Essential conserved3552 rRNA processing protein required for synthesis of 40S ribosomal subunits and 0 processing of the 35
YGR178C   PBP1                            2169 mRNA polyadenylylation
                                                               unknown                M, N, R, controlling PBP1                       0
                    Protein interacting with poly(A)-binding protein Pab1p; likely involved inCcytoplasmthe extent of mRNA polyadenylation; forms a c
YLL036C   PRP19                           1512 with the spliceosome; contains a U-box, motif found in PRP19
                                                                splicing, via factor activity, transesterification mechanism
                                                                                      M, N,
                    Splicing factor associated nuclear mRNARNA splicing spliceosome aR, Ccytoplasm a class of ubiquitin ligases       0
YCR010C   ADY2                             852 transport       transporter
                    Acetate transporter required for normal sporulation activity M, P              Mito        ADY2                   5
YJL005W   CYR1                            6081 meiosis         adenylate cyclase activity
                                                                                      M, P         Mito        CYR1
                    Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; involved0in cell cycle control and
YER166W   DNF1                            4716 endocytosis phospholipid-translocating ATPase activityDNF1
                                                                                      M, P         PM, Mito
                    Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to9           endocytosis, protein tra
YDR039C     ENA2      P-type ATPase sodium pump, involved in Na+ efflux to allow salt P transmembrane movement of ions,10
                                            3276 sodium ion transport activity, coupledtolerance; likely not involved in Li+ efflux
                                                                 ATPase                   M, to       Mito    ENA2                   phosphorylative mechan
YDR508C     GNP1                            1992 amino acid transport acid transporter Cys, Met and
                                                                 amino                    M, P
                      High-affinity glutamine permease, also transports Leu, Ser, Thr, activity Mito Asn; GNP1                      10
                                                                                                               expression is fully dependent on Grr1p and m
YER020W     GPA2                            1350 sporulation
                      Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in respo
                                                                 GTPase activity          M, P        Mito    GPA2                   0
YNL268W     LYP1                            1836 basic amino acid transport acid transporter Lyp1p)
                                                                 basic amino              Can1p, Mito         LYP1                  11
                      Lysine permease; one of three amino acid permeases (Alp1p, M, P activity responsible for uptake of cationic amino acids
YLR305C     STT4                            5703 actin cytoskeleton organization and biogenesisMito
                                                                 1-phosphatidylinositolM, P4-kinase activity  STT4                   0
                      Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell w
YIL047C     SYG1      Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency
                                            2709 signal transduction
                                                                 unknown                  M, P        Mito    SYG1                   5
YMR192W     GYL1                            2163 ER to Golgi transport binding
                                                                 protein                  M, polarized exocytosis; stimulates Gyp5p0
                      Putative GTPase activating protein (GAP) that may have a role inP, G, Ccytoplasm GYL1                           GAP activity on Ypt1p,
YBL038W     MRPL16    Mitochondrial ribosomal protein ofbiosynthesis
                                              699 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL16                 0
YBR122C     MRPL36    Mitochondrial ribosomal protein ofbiosynthesis
                                              534 protein the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL36                 0
YBR268W     MRPL37    Mitochondrial ribosomal protein ofbiosynthesis
                                              318 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL37                 0
YBR282W     MRPL27    Mitochondrial ribosomal protein ofbiosynthesis
                                              441 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL27                 0
YCR003W     MRPL32    Mitochondrial ribosomal protein ofbiosynthesis
                                              552 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL32                 0
YCR046C     IMG1      Mitochondrial ribosomal protein ofbiosynthesis
                                              510 protein the large subunit, required for respiration and for IMG1
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito                           0
                                                                                                               maintenance of the mitochondrial genome
YDL202W     MRPL11    Mitochondrial ribosomal protein ofbiosynthesis
                                              750 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL11                 0
YDR041W     RSM10     Mitochondrial ribosomal protein ofbiosynthesis
                                              612 protein the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike m
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    RSM10                  0
YDR116C     MRPL1     Mitochondrial ribosomal protein ofbiosynthesis
                                              858 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL1                  0
YDR175C     RSM24     Mitochondrial ribosomal protein ofbiosynthesis
                                              960 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    RSM24                  0
YDR237W     MRPL7     Mitochondrial ribosomal protein ofbiosynthesis
                                              879 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL7                  0
YDR322W     MRPL35    Mitochondrial ribosomal protein ofbiosynthesis
                                            1104 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL35                 0
YDR347W     MRP1      Mitochondrial ribosomal protein ofbiosynthesis
                                              966 protein the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRP1                   0
YDR405W     MRP20     Mitochondrial ribosomal protein ofbiosynthesis
                                              792 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRP20                  0
YFR049W     YMR31     Mitochondrial ribosomal protein ofbiosynthesis
                                              372 protein the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    YMR31                  0
YGL068W     MNP1      Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 riboso
                                              585 aerobic respiration
                                                                 unknown                  M, R        Mito    MNP1                   0
YGL129C     RSM23     Mitochondrial ribosomal protein ofbiosynthesis
                                            1467 protein the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prev
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    RSM23                  0
YGR076C     MRPL25    Mitochondrial ribosomal protein ofbiosynthesis
                                              474 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL25                 0
YGR084C     MRP13     Mitochondrial ribosomal protein ofbiosynthesis
                                            1020 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRP13                  0
YGR162W     TIF4631   Translation initiation2859 translational initiation mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p);
                                             factor eIF4G, subunit of the
                                                                 translation initiation factor activity
                                                                                          M, R        Mito    TIF4631                0
YGR165W     MRPS35    Mitochondrial ribosomal protein ofbiosynthesis
                                            1038 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPS35                 0
YGR220C     MRPL9     Mitochondrial ribosomal protein ofbiosynthesis
                                              810 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL9                  0
YHL004W     MRP4      Mitochondrial ribosomal protein ofbiosynthesis
                                            1185 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRP4                   0
YHL035C     VMR1                            4779 transport
                      Protein of unknown function that may interact with ribosomes, M, R transmembrane movement of substances the ATP-binding
                                                                 ATPase activity, coupled to on co-purification experiments; member of
                                                                                          based       Mito    VMR1                  14
YHR147C     MRPL6     Mitochondrial ribosomal protein ofbiosynthesis
                                              645 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL6                  0
YIL093C     RSM25     Mitochondrial ribosomal protein ofbiosynthesis
                                              795 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    RSM25                  0
YJL063C     MRPL8     Mitochondrial ribosomal protein ofbiosynthesis
                                              717 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL8                  0
YJL096W     MRPL49    Mitochondrial ribosomal protein ofbiosynthesis
                                              486 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL49                 0
YJR101W     RSM26     Mitochondrial ribosomal protein ofbiosynthesis
                                              801 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    RSM26                  0
YJR113C     RSM7      Mitochondrial ribosomal protein ofbiosynthesis
                                              744 protein the small subunit, has similarity to E. coli S7 ribosomal protein
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    RSM7                   0
YKL155C     RSM22     Mitochondrial ribosomal protein ofbiosynthesis
                                            1887 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    RSM22                  0
YKL167C     MRP49     Mitochondrial ribosomal protein ofbiosynthesis
                                              414 protein the large subunit, not essentialR mitochondrial MRP49
                                                                 structural constituent M, ribosome
                                                                                          of for      Mito     translation           0
YKL170W     MRPL38    Mitochondrial ribosomal protein ofbiosynthesis
                                              417 protein the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL38                 0
YKR006C     MRPL13    Mitochondrial ribosomal protein ofbiosynthesis
                                              795 protein the large subunit, not essentialR mitochondrial MRPL13
                                                                 structural constituent M, ribosome
                                                                                          of for      Mito     translation           0
YKR085C     MRPL20    Mitochondrial ribosomal protein ofbiosynthesis
                                              588 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL20                 0
YLR312W-A   MRPL15    Mitochondrial ribosomal protein ofbiosynthesis
                                              859 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL15                 0
YLR439W     MRPL4     Mitochondrial ribosomal protein ofbiosynthesis
                                              960 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL4                  0
YML025C     YML6      Mitochondrial ribosomal protein ofbiosynthesis
                                              960 protein the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    YML6                   0
YMR158W     MRPS8     Mitochondrial ribosomal protein ofbiosynthesis
                                              468 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPS8                  0
YMR188C     MRPS17    Mitochondrial ribosomal protein ofbiosynthesis
                                              714 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPS17                 0
YMR193W     MRPL24    Mitochondrial ribosomal protein ofbiosynthesis
                                              777 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL24                 0
YNL005C     MRP7      Mitochondrial ribosomal protein ofbiosynthesis
                                            1116 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRP7                   0
YNL137C     NAM9      Mitochondrial ribosomal component of the small subunit
                                            1461 protein biosynthesis
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    NAM9                   0
YNL177C     MRPL22    Mitochondrial ribosomal protein ofbiosynthesis
                                              930 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL22                 0
YNL185C     MRPL19    Mitochondrial ribosomal protein ofbiosynthesis
                                              477 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL19                 0
YNL208W     YNL208W                           600 unknown
                      Protein of unknown function; may interact with ribosomes, based R co-purification YNL208W
                                                                 unknown                  M, on       Mito                           0
                                                                                                               experiments; authentic, non-tagged protein is
YNL252C     MRPL17    Mitochondrial ribosomal protein ofbiosynthesis
                                              846 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL17                 0
YNL284C     MRPL10    Mitochondrial ribosomal protein ofbiosynthesis
                                              969 protein the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL10                 0
YNL306W     MRPS18    Mitochondrial ribosomal protein ofbiosynthesis
                                              654 protein the small subunit; essential M, ribosome unlike most other mitoribosomal proteins
                                                                 structural constituent of R
                                                                                          for viability,
                                                                                                      Mito    MRPS18                 0
YNR022C     MRPL50    Mitochondrial ribosomal protein ofbiosynthesis
                                              420 protein the large subunit, not essentialR mitochondrial MRPL50
                                                                 structural constituent M, ribosome
                                                                                          of for      Mito     translation           0
YOR150W     MRPL23    Mitochondrial ribosomal protein ofbiosynthesis
                                              492 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    MRPL23                 0
YOR158W     PET123    Mitochondrial ribosomal protein ofbiosynthesis
                                              957 protein the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3
                                                                 structural constituent M, ribosome
                                                                                          of R        Mito    PET123                 0
YPL118W   MRP51     Mitochondrial ribosomal protein ofbiosynthesis
                                          1035 protein the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3
                                                                 structural constituent M, ribosome
                                                                                         of R        Mito        MRP51                 0
YPL173W   MRPL40    Mitochondrial ribosomal protein ofbiosynthesis
                                            894 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                         of R        Mito        MRPL40                0
YEL034W   HYP2                              474 translational initiation binding
                                                                 protein                 M, peptide bond; similar to                   0
                    Translation initiation factor eIF-5A, promotes formation of the firstR, C cytoplasm HYP2 and functionally redundant with Anb1p
YFR044C   YFR044C                         1446 unknown           unknown                 M, R, C cytoplasm glutathione                 0
                    Probable di- and tri-peptidase; forms a complex with Dug2p and Dug3p to degrade YFR044C (GSH) and other peptides contain
YGR285C   ZUO1                            1302 protein folding unfolded protein binding Ssz1p and the Ssb proteins, as a chaperone for nascent polyp
                                                                                         M,
                    Cytosolic ribosome-associated chaperone that acts, together withR, C cytoplasm ZUO1                                0
YOR227W   YOR227W                         3741 unknown           unknown                 based on cytoplasm YOR227W                    0
                    Protein of unknown function that may interact with ribosomes, M, R, C co-purification experiments; authentic, non-tagged prote
YDR477W   SNF1                            1902 protein amino acid phosphorylation M, V, N, activity
                                                                 AMP-activated protein kinase Ccytoplasm and members of the Sip1p/Sip2p/Gal83p fam
                    AMP-activated serine/threonine protein kinase found in a complex containing Snf4pSNF1                              0
YLR084C   RAX2                            3663 bud site selection
                    N-glycosylated protein involved in the maintenance of bud siteM, W
                                                                 unknown                             Mito        RAX2                  1
                                                                                          selection during bipolar budding; localization requires Rax1p
YML052W   SUR7                              909 sporulation      unknown                  associated with        SUR7                  4
                    Putative integral membrane protein; component of eisosomes;M, W, P Mito endocytosis, along with Pil1p and Lsp1p; sporulat
YER015W   FAA2                            2235 synthetase; accepts a wider range of acyl chain lengths than Faa1p, preferring0
                    Long chain fatty acyl-CoA lipid metabolism                           M, X
                                                                 long-chain-fatty-acid-CoA ligaseMito  activity FAA2                    C9:0-C13:0; involved in
YML042W   CAT2      Carnitine acetyl-CoA transferase presentcarnitinemitochondria M, X peroxisomes, transfers activated acetyl groups to carnitine to
                                          2013 carnitine metabolism O-acetyltransferase activity
                                                                   in both                and        Mito        CAT2                  0
YPR135W   CTF4                            2784 DNA repair
                    Chromatin-associated protein, required for sister chromatid cohesion; interacts withCTF4 polymerase alpha (Pol1p) and may link
                                                                 DNA binding             N           nucleus      DNA                  0
YBR009C   HHF1                              312 chromatin assemblybinding
                                                                 DNA or HHF2); core                  nuclear nucleosome assembly and chromosome functio
                    One of two identical histone H4 proteins (see alsodisassemblyNhistone required for HHF1       chromatin            0
YNL030W   HHF2                              312 chromatin assemblybinding
                                                                 DNA or HHF1); core                  nuclear nucleosome assembly and chromosome functio
                    One of two identical histone H4 proteins (see alsodisassemblyNhistone required for HHF2       chromatin            0
YBR010W   HHT1                              411 chromatin assemblybinding
                                                                 DNA or HHT2); core                  nucleus chromatin assembly, 0
                    One of two identical histone H3 proteins (see alsodisassemblyNhistone required for HHT1                            involved in heterochrom
YNL031C   HHT2                              411 chromatin assemblybinding
                                                                 DNA or HHT1); core                  nuclear nucleosome assembly, 0
                    One of two identical histone H3 proteins (see alsodisassemblyNhistone required for HHT2       chromatin            involved in heterochrom
YDR225W   HTA1                              399 DNA also
                    One of two nearly identical (seerepairHTA2) histone H2A subtypes; core nuclear nucleosomechromatin assembly and chromosom
                                                                 DNA binding             N                       HTA1
                                                                                                     histone required for              0
YBL003C   HTA2                              399 DNA also
                    One of two nearly identical (seerepairHTA1) histone H2A subtypes; core nuclear nucleosomechromatin assembly and chromosom
                                                                 DNA binding             N                       HTA2
                                                                                                     histone required for              0
YDR224C   HTB1                              396 chromatin assemblybinding
                                                                 DNA H2B subtypes required for chromatin assembly and chromosome function; Rad6p
                    One of two nearly identical (see HTB2) histone or disassemblyN                               HTB1
                                                                                                     nuclear nucleosome                0
YBL002W   HTB2                              396 chromatin assemblybinding
                                                                 DNA H2B subtypes required for chromatin assembly and chromosome function; Rad6p
                    One of two nearly identical (see HTB1) histone or disassemblyN                               HTB2
                                                                                                     nuclear nucleosome                0
YPR052C   NHP6A                             282 regulation of transcriptionfunctionallypolymerasewith Nhp6Bp; homologous to mammalian high mobility
                                                                 chromatin from RNANredundant II promoter
                    High-mobility group non-histone chromatin protein, binding                                   NHP6A
                                                                                                     nuclear chromatin                 0
YPL043W   NOP4                            2058 rRNA processing binding
                                                                 RNA                     N           nucleolus NOP4                    0
                    Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent o
YGL092W   NUP145    Essential nucleoporin, catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subc
                                          3954 mRNA-nucleus export molecule activity
                                                                 structural              N           nuclear poreNUP145                0
YBL079W   NUP170    Abundant subunit of4509 mRNA-nucleus export molecule activity
                                           the nuclear pore complex (NPC), required for proper localization of specific nucleoporins within the NPC, inv
                                                                 structural              N           nuclear poreNUP170                0
YML103C   NUP188    Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, al
                                          4968 mRNA-nucleus export molecule activity
                                                                 structural              N           nuclear poreNUP188                0
YJR042W   NUP85     Subunit of the Nup84p subcomplex of the export pore complex (NPC), required pore
                                          2235 mRNA-nucleus nuclear molecule activity
                                                                 structural              N                         assembly of the subcomplex and also for for
                                                                                                     nuclear forNUP85                  0
YNL262W   POL2      Catalytic subunit of DNA polymerasesilencing one of the majorN activity
                                          6669 chromatin epsilon, DNA polymerase
                                                                 epsilon                             replication POL2
                                                                                                                 fork                  0
                                                                                          chromosomal DNA replication polymerases characterized by proc
YPL022W   RAD1                            3303 DNA recombination single-stranded DNA N   specific endodeoxyribonuclease activity 0 repair and double-st
                                                                                                     nucleotide RAD1 repair excision
                                                                                                                 excision
                    Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotidefactor 1 complex
YDR195W   REF2                             involved in processing binding
                                                                 RNA                     N           nucleus REF2                      0
                    RNA-binding protein1602 mRNAthe cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation
YNL248C   RPA49                           1248 A49               DNA-directed RNA polymerase activity
                                                                                         N                       RPA49
                    RNA polymerase I subunittranscription from RNA polymerase I promoter DNA-directed RNA Pol I complex                0
YHR031C   RRM3                            2172 mitochondrial genome maintenance
                                                                 DNA Ty1 transposition;              nuclear telomeric heterochromatin
                                                                                                                 RRM3                  0
                    DNA helicase involved in rDNA replication andhelicase activityN relieves replication fork pauses at telomeric regions; structurall
YLR033W   RSC58                           1509 chromatin complex 58KDa subunit; N
                    Remodels the structure ofchromatin remodelingunknown                               RemodelingRSC58
                                                                                         ChromatinRSC complexComplex subunit 0
YHR119W   SET1      Histone methyltransferase, subunit of the COMPASS (Set1C) N
                                          3243 chromatin silencing lysine N-methyltransferase activity SET1 histone H3 on lysine 4; required in trans
                                                                 histone                             COMPASS(H3-K4 complex
                                                                                         complex which methylates specific)            0
YJL168C   SET2                            2202 with a role in transcriptional elongation, methylates a lysine residue of histone 0 associates with the
                                                                 histone methyltransferase activity
                    Histone methyltransferaseregulation of transcription, DNA-dependent  N           nucleus SET2                      H3;
YCR033W   SNT1                            3681 histone complex that
                                                                 NAD-dependent histone deacetylase activity
                                                                                         N            Set3C deacetylase complex 0
                                                                                                                 SNT1
                    Subunit of the Set3C deacetylase deacetylation interacts directly with thehistone subunit, Sif2p; putative DNA-binding protein
YDR310C   SUM1                            3189 chromatin silencing
                                                                 transcriptional of middle sporulation-specific genes; involved in telomere maintenance,
                                                                                         N
                    Transcriptional repressor required for mitotic repressionrepressor activitynucleus SUM1                            0
YCR042C   TAF2                            4224 transcription initiation from RNA polymerase TF TFIID TAF2
                                                                 general RNA II transcription initiation
                                                                                         N           promoter
                    TFIID subunit (150 kDa), involved in RNA polymerase polymerase II TFIIactivity complex                             0
YOR230W   WTM1                            1314 regulation regulation
                                                                 transcription corepressorsilencing; nucleus WTM1
                    Transcriptional repressor involved in of meiosis of meiosis andN activitycontains WD repeats                       0
YLR131C   ACE2                            2313 G1-specific transcription in G1-specificcycle localizes ACE2
                                                                 transcriptional activator C
                                                                                         N, genes,
                    Transcription factor that activates expression of early mitotic cell activity nucleus to daughter cell nuclei 0    after cytokinesis and de
YLR153C   ACS2                            2052 acetyl-CoA along with Acs1p, is theN, C
                                                                 acetate-CoA ligase activity source of acetyl-coA for histone acetlyation; mutants affec
                    Acetyl-coA synthetase isoform which, biosynthesis                     nuclear cytosol        ACS2                  0
YGR204W   ADE3                            2841 purine C1-tetrahydrofolate
                                                                 formate-tetrahydrofolate C
                                                                                         N, ligase cytoplasm ADE3
                                                                                                      in single                        0
                    Cytoplasmic trifunctional enzymebase biosynthesis synthase, involvedactivity carbon metabolism and required for biosynthes
YJR105W   ADO1                            1023 purine base metabolism kinase activity
                                                                 adenosine               N, C        cytoplasm ADO1                    0
                    Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produce
YJR148W   BAT2                            1131 branched chain family amino homologC murine ECA39; highly
                                                                 branched-chain-amino-acid transaminase BAT2
                                                                                         N, of                                         0
                    Cytosolic branched-chain amino acid aminotransferase,acid biosynthesiscytoplasm activity expressed during stationary phase an
YLR438W   CAR2                            1275 arginine catalyzesornithine-oxo-acid transaminase degradation, expression is dually-regulated by allophan
                                                                                         of C          activity
                    L-ornithine transaminase (OTAse),catabolismthe second step N, argininecytoplasm CAR2                               0
YHL030W   ECM29                            the proteasome; tethers
                                                                 protein proteasome core particle to the regulatory particle, and enhances the stability o
                    Major component of 5607 protein catabolism thebinding                N, C        cytoplasm ECM29                   0
YJL157C   FAR1                            2493 signal transduction during conjugation with cellular to pheromone;
                                                                 cyclin-dependent proteinC response fusion
                                                                                         N,           inhibitor activity
                    Cyclin-dependent kinase inhibitor that mediates cell cycle arrest inkinasecytoplasm FAR1 also forms a 0            complex with Cdc24p, S
YOR375C   GDH1                              glutamate dehydrogenase, synthesizes glutamate from activity
                                                                 glutamate               N, C        cytoplasm GDH1                    0
                    NADP(+)-dependent1365 glutamate biosynthesis dehydrogenase (NADP+) ammonia and alpha-ketoglutarate; rate of alpha-ket
YDR174W   HMO1                              741 plasmid maintenance
                                                                 single-stranded DNA N, involved in genome maintenance; rDNA-binding component of t
                    Chromatin associated high mobility group (HMG) family member C       binding cytoplasm HMO1                        0
YDL125C   HNT1                              588 nucleotide metabolism activity
                                                                 hydrolase
                    Adenosine 5'-monophosphoramidase; interacts physically andN, C                   cytoplasm HNT1                    0
                                                                                          genetically with Kin28p, a CDK and TFIIK subunit, and genetically
YDR158W   HOM2                            1098 methionine metabolism
                                                                 aspartate-semialdehyde CN, dehydrogenase activitypathway for methionine and threonine bi
                                                                                                     in the common
                    Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step cytoplasm HOM2                                0
YJR139C   HOM6                            1080 methionine metabolism
                                                                 homoserine dehydrogenase activity
                                                                                         N, C        cytoplasm HOM6                    1
                    Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common
YBL105C   PKC1                            3456 cell wall organization kinase C activity during growth; localized to sites of polarized growth and the mo
                                                                  for cell wall remodeling
                    Protein serine/threonine kinase essentialproteinand biogenesis C     N,          cytoplasm PKC1                    0
YIL053W   RHR2                              816 response to osmotic stress
                                                                 glycerol0-phosphatase involved in glycerol biosynthesis, induced in response to both an
                                                                                         N, C
                    Constitutively expressed isoform of DL-glycerol-3-phosphatase; activity cytoplasm RHR2                             0
YGR180C   RNR4                            1038 DNA replicationibonucleoside-diphosphate complex catalyzes
                                                                 r                       N, RNR cytoplasm RNR4
                    Ribonucleotide-diphosphate reductase (RNR), small subunit; the C reductase activity the rate-limiting0step in dNTP synthesis
YER103W   SSA4                            1929 highly inducedunfolded protein binding
                                                                  upon                   role        cytoplasm SSA4                    0
                    Heat shock protein that is response to stress stress; plays a N, Cin SRP-dependent cotranslational protein-membrane targeting
YNL309W   STB1                              regulation transitiontranscriptional activator activity cytoplasm STB1
                                                                   of mitotic cell cycle N, C                                          0
                    Protein with a role in1263 G1/S of MBF-specific transcription at Start, phosphorylated by Cln-Cdc28p kinases in vitro; unphospho
YDR146C   SWI5                            2130 G1-specific transcription in mitotic cell cyclethe M/G1 phase boundary and in G1 phase; localization to
                                                                 transcriptional expressed at
                                                                                         N, C
                    Transcription factor that activates transcription of genesactivator activity cytoplasm SWI5                        0
YCR053W   THR4                              conserved protein threonine synthase activityN, C        cytoplasm THR4
                    Threonine synthase,1545 endocytosis that catalyzes formation of threonine from 0-phosphohomoserine;0expression is regulated
YDR213W   UPC2                            2742 sterol biosynthesis polymerase II TF activity transport and biosynthetic genes; involved in the anaero
                                                                 RNA
                    Sterol regulatory element binding protein, induces transcription ofC N, sterol cytoplasm UPC2                      0
YGL082W     YGL082W   Hypothetical protein1146 unknown             unknown                 N, C        cytoplasm YGL082W                 0
YOR131C     YOR131C   Hypothetical protein 657 unknown             unknown                 N, C        cytoplasm YOR131C                 0
YBR046C     ZTA1                            1005 unknown           unknown
                      Zeta-crystallin homolog, found in the cytoplasm and nucleus; hasC                cytoplasm ZTA1                    0
                                                                                           N, similarity to E. coli quinone oxidoreductase and to human zeta-
YHR010W     RPL27A                              the protein biosynthesis
                                                                   structural constituent N, ribosome Rpl27Bp and has similarity to 0 L27 ribosomal prote
                                                                                           of C, R to
                      Protein component of972 large (60S) ribosomal subunit, nearly identical cytosol              RPL27A                rat
YGL103W     RPL28                             961 protein biosynthesis
                                                                    ribosomal subunit, has ribosome
                                                                                           of C, R nucleus L15 and                       0
                      Ribosomal protein L29 of the large (60S)structural constituent N, similarity to E. coli RPL28 rat L27a ribosomal proteins; may ha
YNL258C     DSL1                            2265 retrograde transport, Golgi to ER N, E
                                                                   unknown                             ER          DSL1                  0
                      Endoplasmic reticulum (ER)-localized peripheral membrane protein required for Golgi-to-ER retrograde traffic; component of the
YER120W     SCS2      Integral ER membrane protein that regulates phospholipid metabolism via an interaction with the FFAT motif of Opi1p, also invol
                                              735 chromatin silencing binding
                                                                   protein                 N, E        ER          SCS2                  1
YEL002C     WBP1      Beta subunit of the oligosaccharylamino acid N-linkedglycoprotein complex; required for N-linked glycosylation of proteins in the e
                                            1293 protein transferase (OST) glycosylation
                                                                   dolichyl-diphosphooligosaccharide-protein WBP1
                                                                                           N, E        ER                                1
                                                                                                                   glycotransferase activity
YDL126C     CDC48                           2508 ubiquitin-dependent with homology N, E, C nucleus CDC48
                                                                   ATPase activity         to                                            0
                      ATPase in ER, nuclear membrane and cytosolprotein catabolismmammalian p97; in a complex with Npl4p and Ufd1p participate
YKR095W     MLP1      Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved w
                                            5628 mRNA-nucleus export
                                                                   ribonucleoprotein binding
                                                                                           N, R                    MLP1
                                                                                                       nuclear membrane                  0
YDL014W     NOP1                              984 rRNA the small subunit processome complex,nucleolusrequired for processing0 pre-18S rRNA; has s
                      Nucleolar protein, component of modification                         N, R
                                                                   methyltransferase activity           which is NOP1                     of
YMR093W     UTP15     Nucleolar protein, component of the small subunitbinding processome containing the U3 snoRNA that is involved in processing o
                                            1542 processing of 20S pre-rRNA
                                                                   snoRNA (SSU)            N, R        sNRP        UTP15                 0
YBL004W     UTP20                           7482 35S primary processome, which N, R, C
                                                                   snoRNA binding                       the biogenesis of
                      Component of the small-subunit (SSU)transcript processingis involved incytoplasm UTP20 the 18S rRNA 0
YMR226C     YMR226C                           804 metabolism oxidoreductase activity, acting on the aldehyde or acids; green donors, NAD or NADP a
                                                                                           N, R, C cytoplasm YMR226C                     0
                      NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy oxo group of fluorescent protein fusion
YBR038W     CHS2                            2892 cytokinesis       chitin synthase activityO           bud neck CHS2                     7
                      Chitin synthase II, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to c
YDL223C     HBT1      Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating HBT1
                                            3141 cellular morphogenesis during conjugation with cellular fusion
                                                                   unknown                 O                       projection); mutants are defective for mating
                                                                                                       mating projection                 0
YGR238C     KEL2                            2649 complex with Kel1p to negatively O
                                                                   unknown                             bud neck KEL2                     0
                      Protein that functions in a conjugation with cellular fusion regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to reg
YJR065C     ARP3      Essential component of the Arp2/3 complex, whichconstituent O cytoskeleton nucleation center required 0 the motility and inte
                                            1350 actin filament organization a highly conservedArp2/3 protein complex
                                                                   structural is           of           actin      ARP3                  for
YMR319C     FET4      Low-affinity Fe(II) transporter of the plasma membrane
                                            1659 intracellular copperion transporter activity
                                                                   iron ion transport      P           PM          FET4                  7
YGR032W     GSC2      Catalytic subunit of 1,3-beta-glucan synthase, involved insynthase activity inner layer of the spore wall; activity positively regulate
                                            5688 cell wall organization and biogenesis
                                                                   1,3-beta-glucan formation of the
                                                                                           P                       GSC2
                                                                                                       prospore membrane                14
YMR011W     HXT2      High-affinity glucose1626 hexose transport facilitator superfamily, expression is induced by low levels of glucose and repressed
                                              transporter of the major
                                                                   glucose transporter activity
                                                                                           P           PM          HXT2                 12
YDR345C     HXT3      Low affinity glucose 1704 hexose transport facilitator superfamily, expression is induced in low or high glucose conditions
                                             transporter of the major
                                                                   glucose transporter activity
                                                                                           P           PM          HXT3                 11
YPL058C     PDR12                           4536 transport         xenobiotic-transporting
                      Plasma membrane weak-acid-inducible ATP-binding cassette P ATPase PM              activity PDR12                  11
                                                                                           (ABC) transporter, required for weak organic acid resistance, stron
YML123C     PHO84     High-affinity inorganic phosphate (Pi) transport and ion transporter activity transporter; regulated by Pho4p and Spt7p; mutatio
                                            1764 phosphate transporter
                                                                   manganese low-affinity manganese
                                                                                           P           PM          PHO84                 9
YPL232W     SSO1                              873 vesicle fusion fusion of activity        P           PM          SSO1                  1
                      Plasma membrane t-SNARE involved in t-SNARE secretory vesicles at the plasma membrane and in vesicle fusion during sporul
YNL154C     YCK2                            1641 protein amino casein kinase IIactivity
                                                                    casein kinase isoform;
                      Palmitoylated, plasma membrane-boundacid phosphorylation P sharesPM                           functions with Yck1p 0 morphogenesis, prop
                                                                                                        redundantYCK2                    in
YGR281W     YOR1                            4434 transport         xenobiotic-transporting ATPase PM
                                                                                           P             mediates export                11
                      Plasma membrane transporter of the ATP-binding cassette (ABC) family,activity YOR1 of many different organic anions includ
YNL323W     LEM3                            1245 cell surface receptor linked signal transduction
                                                                   transcription regulatorin translocation of phospholipids and alkylphosphocholine drugs a
                                                                                             activity
                      Membrane protein of the plasma membrane and ER, involved P, E, C cytoplasm LEM3                                    2
YMR183C     SSO2                              888 vesicle fusion fusion of activity        P, E, at cytoplasm SSO2                       1
                      Plasma membrane t-SNARE involved in t-SNARE secretory vesiclesC the plasma membrane; syntaxin homolog that is functiona
YOR171C     LCB4                            1875 sphingolipid metabolism
                                                                   D-erythro-sphingosine kinase CER
                                                                                           P, G, E, activity       LCB4                  0
                      Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecul
YPL204W     HRR25                           1485 regulating        casein kinase activityP, N          nucleus HRR25
                      Protein kinase involved in DNA repairdiverse events including vesicular trafficking, gene expression, DNA 0        repair, and chromosom
YIR006C     PAN1                            4443 endocytosis protein binding, bridgingN    P,          PM          actin                 0
                      Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with PAN1patches on the cell cortex; promotes pro
YJR059W     PTK2                            2457 G1/S transitionprotein in cell cycle of N, transport across plasma membrane; 0
                                                                     of mitotic regulation
                      Putative serine/threonine protein kinase involvedkinase activityP, ionC cytoplasm PTK2                             enhances spermine up
YBR118W     TEF2                            1377 translational elongation elongationR
                                                                   translation              factor activity
                                                                                                       ribosome TEF2                     0
                      Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA
YDR385W     EFT2                            2529 translational elongation elongationR
                                                                    by EFT1;                factor activity
                                                                                                       ribosome EFT2                     0
                      Elongation factor 2 (EF-2), also encodedtranslationcatalyzes ribosomal translocation during protein synthesis; contains diphtham
YJR047C     ANB1                              474 translational initiation
                                                                   translation initiation first activity
                                                                                           R           ribosome ANB1                     0
                      Translation initiation factor eIF-5A, promotes formation of thefactorpeptide bond; similar to and functionally redundant with Hyp2p
YLR249W     YEF3                            3135 translational elongation elongationR
                                                                   translation              factor activity
                                                                                                       ribosome YEF3                     0
                      Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from
YHR113W     YHR113W                         1473 proteolysis       metalloendopeptidase activity cytoplasm YHR113W
                                                                                           R, C                                          0
                      Cytoplasmic aspartyl aminopeptidase; cleaves unblocked N-terminal acidic amino acid residues from peptide substrates; forms a
YOL070C     YOL070C                         1506 unknown           unknown                 R, on       cytoplasm experiments; green fluorescent protein (GFP)
                      Protein of unknown function; may interact with ribosomes, based C co-purification YOL070C                          0
YBR074W     YBR074W                         2931
                      Putative metalloprotease proteolysis                                 U
                                                                   metalloendopeptidase activity unknown YBR074W                         9
YCR073C     SSK22                           3996 protein amino mitogen-activated signalingactivity
                                                                   MAP kinase kinase kinase pathway; functionally redundant with, and homologous to, S
                      MAP kinase kinase kinase of the HOG1 acid phosphorylation U                      unknown SSK22                     0
YDR089W     YDR089W                                                unknown
                      Hypothetical protein2610 membrane organization and biogenesis        U           membraneYDR089W                   3
YDR406W     PDR15                           4590 transport         ATPase activity, coupled general stress response factor substances
                                                                                           U           membranePDR15                    12
                      ATP binding cassette (ABC) transporter of the plasma membrane;to transmembrane movement ofimplicated in cellular detoxifica
YDR502C     SAM2                            1155 methionine metabolism adenosyltransferaseunknown to the
                                                                   methionine              U             activity                        0
                      S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP SAM2 sulfur atom of methionine; one of two d
YIL118W     RHO3      Non-essential small GTPase of filament organization of Ras-like proteins involved in the establishment 0 cell polarity; GTPase
                                              696 actin the Rho/Rac subfamily
                                                                   GTPase activity         U                       R
                                                                                                       intracellular HO3                 of
YKL039W     PTM1                            1596 unknown           unknown
                      Protein of unknown function, copurifies with late Golgi vesiclesU                unknown PTM1
                                                                                            containing the v-SNARE Tlg2p                 7
YLR359W     ADE13                           1449 de novo' IMP biosynthesis
                                                                   adenylosuccinate lyase activity unknown ADE13
                                                                                           U
                      Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway                0
YOR291W     YOR291W                         4419 unknown           unknown                 U             type V P-type ATPase           11
                      Putative protein of unknown function; shares sequence similarity with themembraneYOR291W Spf1p; YOR291W is not an esse
YBR127C     VMA2                            1554 vacuolar acidification
                                                                   hydrogen-transporting of the vacuolar rotational mechanism an
                                                                                           V           vacuole VMA2
                      Subunit B of the eight-subunit V1 peripheral membrane domainATPase activity,H+-ATPase (V-ATPase), 0 electrogenic proton p
YBR139W     YBR139W                          Carboxypeptidase; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YBR139W is not
                      Putative serine type 1527 unknown                                    V
                                                                   carboxypeptidase C activity         vacuole YBR139W                   0
YCL005W-A   VMA9                               subunit e of acidification activity, coupled to transmembrane movement of ions 2
                                                                   ATPase                  V           vacuole VMA9
                      Vacuolar H+ ATPase 395 vacuolarthe V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase
YCL038C     ATG22     Protein required for the breakdown of autophagic vesicles in the vacuole vacuole, membrane
                                            1587 protein targeting to vacuole
                                                                   unknown                 V                       ATG22                10
                                                                                                        during autophagy, putative integral membrane protein th
YDL046W     NPC2                              522 unknown          unknown                 V            protein    NPC2
                      Functional homolog of human NPC2/He1, which is a cholesterol-binding vacuolewhose deficiency causes1Niemann-Pick type C2
YDR119W     YDR119W                         2307 unknown           unknown                 V           vacuole YDR119W                  14
                      Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to vacuolar membrane; YDR119W
YDR135C     YCF1                            4548 response to metal of transporter activity
                                                                   bilirubin               V           vacuole YCF1                     14
                      Vacuolar glutathione S-conjugate transporterion the ATP-binding cassette family, has a role in detoxifying metals such as cadmiu
YDR284C     DPP1                              870 signal transduction
                                                                   phosphatidate phosphatase activity
                                                                                           V           vacuole DPP1                      5
                      Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosph
YDR481C     PHO8                              phosphatase, a glycoproteinphosphatasethe
                                                                   alkaline localized to V  activity vacuole PHO8                        1
                      Repressible alkaline1701 protein amino acid dephosphorylation vacuole; regulated by levels of inorganic phosphate and by a sy
YEL013W     VAC8                            1737 protein targeting to vacuole
                                                                   protein interacts
                      Phosphorylated vacuolar membrane protein thatbinding withV                       vacuole the cytoplasm-to-vacuole targeting (Cvt) path
                                                                                            Atg13p, required for VAC8                    0
YEL051W     VMA8                              771 vacuolar acidification
                                                                   hydrogen-transporting of the vacuolar rotational mechanism an
                                                                                           V           vacuole VMA8
                      Subunit D of the eight-subunit V1 peripheral membrane domainATPase activity,H+-ATPase (V-ATPase), 0 electrogenic proton p
YEL060C     PRB1                               B (yscB), a serine serine-type endopeptidase activity
                                                                                             V          vacuole PRB1                          the
                        Vacuolar proteinase1908 sporulation protease of the subtilisin family; involved in protein degradation in 0 vacuole and require
YFL041W     FET5                              1869 iron ion transportprotein with similarity to         vacuole FET5
                        Multicopper oxidase, integral membrane ferroxidase activity V Fet3p; may have a role in iron transport 0
YGL006W     PMC1        Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ATPase activity
                                              3522 calcium ion homeostasis
                                                                     calcium-transporting ions; prevents growthPMC1
                                                                                             V          vacuole                              9
                                                                                                                      inhibition by activation of calcineurin in the pr
YGL212W     VAM7                               951 SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; functions with a syntaxin h
                        Component of the vacuolevesicle fusion v-SNARE activity              V          vacuole VAM7                         0
YGR125W     YGR125W                           3111 unknown           unknown                 V          vacuole YGR125W                     10
                        Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; gre
YHR028C     DAP2                              2457 protein processingdipeptidyl-peptidase and tripeptidyl-peptidase activity
                                                                                             V          vacuole DAP2                         1
                        Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
YHR132C     ECM14                             1293 cell similarity to the zinc carboxypeptidase family, required for normal cell wall assembly
                                                                     metalloendopeptidase    V
                        Putative metalloprotease with wall organization and biogenesis activity vacuole ECM14                                1
YJL172W     CPS1                              1731 proteolysis       Gly-X induced underV     low-nitrogen conditions
                        Vacuolar carboxypeptidase yscS; expression iscarboxypeptidase activity vacuole CPS1                                  1
YKL080W     VMA5                              1179 vacuolar acidification
                                                                     hydrogen-transporting of vacuolar H+-ATPase (V-ATPase), an 0
                                                                                             V          vacuole VMA5
                        Subunit C of the eight-subunit V1 peripheral membrane domainATPase activity, rotational mechanism electrogenic proton pum
YKL103C     LAP4                              1545 often used as aminopeptidase I in studies of autophagy and cytosol to vacuole 0
                                                                      a marker protein activity
                        Vacuolar aminopeptidase,vacuolar protein catabolism                  V          vacuole LAP4                          targeting (CVT) pathwa
YLL015W     BPT1                              4680 transporter of bilirubin transporter found in vacuolar membrane, involved in the transport of unconjugat
                        ABC type transmembranebilirubin transport                            V
                                                                     MRP/CFTR family, activity          vacuole BPT1                        14
YLL048C     YBT1                              4986 bile acid transport
                                                                     (ABC) family involvedV to acid transport; movement of substances
                                                                                               in       vacuole YBT1                        14
                        Transporter of the ATP-binding cassette ATPase activity, coupledbiletransmembranesimilar to mammalian bile transporters
YLR034C     SMF3                              1422 intracellular involved in iron homeostasis; transcriptionally regulated by metal 10 member of the Nra
                                                                     iron ion transporter activity
                        Putative divalent metal ion transporter sequestering of iron ion V              vacuole SMF3                        ions;
YMR160W     YMR160W                           2451 unknown           unknown                 V          vacuole YMR160W                      0
                        Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; m
YMR243C     ZRC1                               zinc zinc ion homeostasis
                                                                     di-, tri-valent inorganic
                                                                                             cytosol transporter activity
                                                                                                        vacuole ZRC1                         6
                        Vacuolar membrane1329 transporter, transports zinc from the Vcation into the vacuole for storage; also has a role in resistance to
YNL115C     YNL115C                           1935 unknown           unknown                 V          vacuole YNL115C                      5
                        Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not a
YNL217W     YNL217W     Hypothetical protein 981 unknown                                     V          vacuole
                                                                     phosphoric monoester hydrolase activity YNL217W                         1
YNL321W     VNX1                              2727 ion transport antiporter
                        Protein of unknown function, potential Cdc28p substrate              V          vacuole VNX1                        13
YNR013C     PHO91                             2685 phosphate transport
                                                                     phosphate transporter activity membranePHO91
                                                                                             V                                              12
                        Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription in
YOL060C     MAM3                              2121 mitochondrial organization has similarity
                                                                       morphology,           V          vacuole
                        Protein required for normal mitochondrialunknown and biogenesis to hemolysins MAM3                                   3
YOR087W     YVC1                              2028 cation homeostasiscalcium channel the V
                        Vacuolar cation channel, mediates release of Ca(2+) fromactivity                  response to hyperosmotic shock
                                                                                              vacuole invacuole YVC1                         7
YOR270C     VPH1                              2523 vacuolar acidification
                                                                     hydrogen-transporting ATPase activity, rotational is located in 7
                                                                                             V          vacuole Vph1p
                        Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); VPH1 mechanism V-ATPase complexes of t
YOR332W     VMA4                               702 vacuolar acidification
                                                                     hydrogen-transporting of the vacuolar rotational mechanism an
                                                                                             V          vacuole VMA4
                        Subunit E of the eight-subunit V1 peripheral membrane domainATPase activity,H+-ATPase (V-ATPase), 0 electrogenic proton p
YPL006W     NCR1                               protein that transits sphingolipid transporter vacuolar protein NCR1
                                                                      through the            V                                              14
                        Vacuolar membrane3513 sphingolipid metabolism biosyntheticactivity vacuole sorting pathway, involved in sphingolipid meta
YPR036W     VMA13                             1437 vacuolar acidification
                                                                     hydrogen-transporting of the vacuolar rotational mechanism an
                                                                                             V          vacuole VMA13
                        Subunit H of the eight-subunit V1 peripheral membrane domainATPase activity,H+-ATPase (V-ATPase), 0 electrogenic proton p
YNL054W     VAC7        Integral vacuolar membrane protein involved in vacuole inheritance and morphology; may function to regulate Fab1p kinase activ
                                              3498 vacuole inheritance
                                                                     enzyme regulator activity
                                                                                             V, C                    VAC7
                                                                                                        vacuole, cytoplasm                   1
YMR297W     PRC1                              1599 vacuolar protein catabolism
                                                                     carboxypeptidase C activity
                                                                                             V, E       vacuole PRC1                         0
                        Vacuolar carboxypeptidase Y (proteinase C), involved in protein degradation in the vacuole and required for full protein degradati
YJL012C     VTC4                               protein involved in vacuolar polyphosphate accumulation; functions as a regulator of3
                        Vacuolar membrane2166 vacuole fusion,unknown    non-autophagic       V, E       vacuole VTC4                           vacuolar H+-ATPase a
YLR220W     CCC1                               969 iron ion homeostasis
                                                                     manganese ion transporter
                                                                                             V, deficit of
                                                                                                        vacuole CCC1                         3
                        Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratoryG activityyfh1 mutants, which lack the ortholog of mammalian f
YBR162C     TOS1                              1368 protein of        unknown                 V, as      cell wall TOS1                       0
                        Covalently-bound cell wallunknown unknown function; identified W a cell cycle regulated SBF target gene; deletion mutants are
YBR286W     APE3                              1692 Y, processed to catabolism
                                                                     aminopeptidase activity
                        Vacuolar aminopeptidase vacuolar protein mature form by Prb1p W      V,         vacuole APE3                         0
YGR282C     BGL2                               942 major protein of the and biogenesis
                                                                     glucan 1,3-beta-glucosidase activitywall BGL2
                                                                                             W          cell
                        Endo-beta-1,3-glucanase,cell wall organizationcell wall, involved in cell wall maintenance                           0
YLR314C     CDC3                              1563 cell wall organization neck that is required for prospore membranerecruit proteins to the neck and can
                                                                     structural constituent W
                        Component of the septin ring of the mother-budand biogenesisof cytoskeleton                  CDC3
                                                                                                        cytokinesis; septins                 0
YDR261C     EXG2                              1689 cell wall organization and biogenesis
                                                                     glucan 1,3-beta-glucosidase activitywall EXG2
                                                                                             W            be                                 0
                        Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; maycell anchored to the plasma membrane via a glycosylph
YGR279C     SCW4                              1161 conjugation with cellular scw10
                                                                     glucosidase activity W             cell wall SCW4
                        Cell wall protein with similarity to glucanases; scw4fusion double mutants exhibit defects in mating                 0
YEL040W     UTR2                              1404 cell wall organization and biogenesis
                                                                     unknown                 W          cell wall to the                     0
                        Putative glycosidase, glycosylphosphatidylinositol (GPI)-anchored protein localized UTR2 bud neck; has a role in cell wall mainte
YMR305C     SCW10                             1170 conjugation with cellular play a
                                                                     glucosidase activity W, E, C cytoplasm mating                           0
                        Cell wall protein with similarity to glucanases; may fusion role in conjugation during SCW10based on mutant phenotype and its re
YDL078C     MDH3                              dehydrogenase, catalyzes interconversion of activityand
                                                                     L-malate                X                       MDH3                    0
                        Cytoplasmic malate 1032 fatty acid beta-oxidation dehydrogenase malatePero oxaloacetate; involved in the glyoxylate cycle
YGL153W     PEX14       Peroxisomal membrane protein that is a protein component of X peroxisomal protein import machinery, 0
                                              1026 protein targeting to Pero
                                                                     central binding          the       Pero         PEX14                    interacts with PTS1 (Pe
YGL205W     POX1        Fatty-acyl coenzyme A oxidase, involvedacyl-CoA oxidase activity
                                              2247 fatty acid beta-oxidation acid beta-oxidation pathway; localized to the peroxisomal matrix
                                                                       in the fatty          X          Pero         POX1                    0
YGR154C     GTO1                              1071 unknown           unknown
                        Omega-class glutathione transferase; induced under oxidativeX                   Pero         GTO1
                                                                                              stress; putative peroxisomal localization 0
YKR009C     FOX2                              2703 the acid beta-oxidation
                                                                     3-hydroxyacyl-CoA dehydrogenase has 3-hydroxyacyl-CoA dehydrogenase and enoyl-C
                                                                                             X          Pero
                        Multifunctional enzyme of fattyperoxisomal fatty acid beta-oxidation pathway; activityFOX2                           0
YLR151C     PCD1                              1023 unknown
                        Peroxisomal nudix pyrophosphatase withpyrophosphatase activity A and Pero derivatives, may function to remove potentially toxi
                                                                       specificity for coenzyme
                                                                                             X           CoA         PCD1                    0
YMR026C     PEX12                             1200 Pero organization thatbiogenesis X
                                                                     protein plays                      Pero         PEX12                   1
                        RING-finger peroxisomal membrane peroxinand bindingan essential role in peroxisome biogenesis and peroxisomal matrix protei
YOL147C     PEX11                              711 Pero required unknown
                                                                      for and biogenesis X
                        Peroxisomal membrane proteinorganization peroxisome proliferation andPero                    PEX11                   0
                                                                                                          medium-chain fatty acid oxidation, most abundant prote
YPL112C     PEX25                             1185 Pero organization and biogenesis X
                                                                     unknown                            Pero         PEX25                   0
                        Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho
YDL022W     GPD1                              1176 intracellular dehydrogenase, key enzyme of glycerol synthesis, essential for growth under osmotic stre
                                                                     glycerol-3-phosphate X, C          cytosol
                        NAD-dependent glycerol-3-phosphate accumulation of glyceroldehydrogenase (NAD+) activity     GPD1                    0
YDR244W     PEX5                              1839 Pero organization and biogenesis X, Cbinding
                                                                     Pero targeting sequence            cytosol      PEX5                    0
                        Peroxisomal membrane signal receptor for C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required
YNL117W     MLS1                              1665 of the glyoxylate cycle, involved in utilization ofcytoplasm MLS1 carbon sources; expression is subject
                                                                     m
                        Malate synthase, enzyme glyoxylate cycle alate synthase activityC    X,           non-fermentable                    0
YKR046C     PET10                              852 aerobic respiration
                                                                     unknown                            lipid particle ET10
                                                                                                                     P                       0
                        Protein of unknown function that co-purifies with lipid particles;Lexpression pattern suggests a role in respiratory growth; computa
YER048W-A   YER048W-A                          285 unknown
                        Protein required for mitochondrial iron-sulfur cluster biosynthesis
                                                                     unknown                 M          Mito         YER048W-A               0
YHR001W-A   QCR10                              297 aerobic respiration
                                                                     ubiquinol-cytochrome-c reductase aerobic respiration
                                                                                             M
                        Subunit of the ubiqunol-cytochrome c oxidoreductase complex, involved Mito      in activity QCR10                    1
YMR098C     YMR098C                           1839 unknown
                        Protein of unknown function; the authentic, non-tagged proteinM detected in highly YMR098C
                                                                     unknown                   is       Mito                                  in
                                                                                                                     purified mitochondria 0 high-throughput stud
YOR022C     YOR022C                           2148 unknown
                        Protein with similarity to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly 0
                                                                     phospholipase activity  M          Mito         YOR022C                  purified mitochondria in
YDL086W     YDL086W                            822 unknown             authentic, non-tagged protein activity
                                                                                             M, C       cytoplasm in highly purified mitochondria in high-through
                        Putative protein of unknown function; thecarboxymethylenebutenolidaseis detected YDL086W                             0
YKR064W     YKR064W                           2592 unknown            the authentic, non-tagged protein is detected in highly purified mitochondria in high-thro
                        Zinc cluster protein of unknown function; unknown                    M, N, C cytoplasm YKR064W                       0
YLR108C     YLR108C                           1458 unknown           unknown                 N          nucleus YLR108C                      0
                        Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssenti
YBR063C     YBR063C                           1215 unknown           unknown                 U
                        Putative protein of unknown function; YBR063C is not an essential gene unknown YBR063C                               0
YPL100W     ATG21                             3,5-bisphosphate-binding protein requiredV, D, C cytosol,pro-aminopeptidase I, predicted to fold as a seve
                        Phosphatidylinositol1491 autophagy                                     for
                                                                     phosphoinositide binding maturation of vacuole  ATG21                   0
YPL084W     BRO1                            vacuolar protein sorting (VPS) factor that D, C
                                                                  intracellular transportercoordinates deubiquitination
                                                                                                                   pre-VACUOLE         0
                      Cytoplasmic class E2535 ubiquitin-dependent protein catabolismactivity cytoplasm,BRO1 in the multivesicular body (MVB) path
YKR035W-A   DID2      Class E protein of the615 protein targeting to vacuole
                                             vacuolar protein-sorting (Vps) pathway, D, C
                                                                  unknown                             cytoplasm, with the late endosome, has human ortholog
                                                                                                                   late
                                                                                          associates reversiblyDID2endosome            0
YLR083C     EMP70                          2004 transport         transporter activity D              membraneEMP70                   10
                      Protein whose 24kDa cleavage product is found in endosome-enriched membrane fractions, predicted to be a transmembrane pr
YKR039W     GAP1                           permease; localization to the permease activityG, regulated by nitrogen source
                                                                  L-proline               V, P,                   GAP1
                      General amino acid 1809 amino acid transport plasma membrane isDPM, vacuolar lumenal membrane                   12
YOR357C     GRD19                           489 protein late-Golgi
                      Sorting nexin required to maintainlocalizationresident enzymesD, C
                                                                  protein binding                     cytosol     GRD19                0
                                                                                           in their proper location by recycling molecules from the prevacuol
YKL143W     LTV1                           1392 response to osmotic stress
                                                                  unknown                 N, D, R cytoplasm permease Gap1p; required for ribosomal sma
                      Component of the GSE complex, which is required for proper sorting of amino acid LTV1                            0
YPR079W     MRL1                           1146 vacuolar mammalian mannose-6-phosphate receptors, possibly functions as a 2
                                                                  signal
                      Membrane protein with similarity to transport sequence bindingC     D,          cytoplasm MRL1                    sorting receptor in the d
YLR093C     NYV1                             of vesicle fusion v-SNARE activity           V, D        vacuole, endosome
                                                                                                                  NYV1
                      v-SNARE component903the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent 1               Ca(2+) transport activit
YJL053W     PEP8      Vacuolar protein sorting protein that forms part of the multimeric membrane-associated retromer complex along with Vps35p, Vp
                                           1140 protein retention in Golgi
                                                                  unknown                 D                       PEP8
                                                                                                      endosome, golgi                  0
YJL204C     RCY1                           2523 endocytosis protein binding
                      F-box protein involved in recycling plasma membrane proteinsG, D                Golgi, endosome
                                                                                                                  RCY1                 0
                                                                                           internalized by endocytosis; localized to sites of polarized growth
YNL287W     SEC21     Gamma subunit of coatomer, to heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golg
                                           2808 ER a Golgi transport
                                                                  unknown                 G, D                    SEC21
                                                                                                      COPI vesicle coat                0
YIL076W     SEC28     Epsilon-COP subunit of the coatomer; transport retrograde Golgi-to-ER proteinvesicle coat
                                           1080 ER to Golgi regulates
                                                                  unknown                 G, D                    SEC28                1
                                                                                                      COPI traffic; stabilizes Cop1p, the alpha-COP and the c
YDR170C     SEC7                           exchange factor (GEF) forguanyl-nucleotide factors involved activity
                                                                  ARF                     G, D, C cytosol         SEC7                 0
                      Guanine nucleotide 6030 ER to Golgi transportADP ribosylation exchange factor in proliferation of the Golgi, intra-Golgi transport
YLR025W     SNF7                            723 Endosome late to vacuole transport D, C
                                                                  unknown                             cytoplasm,SNF7
                                                                                                                   endosome            0
                      One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmemb
YJL036W     SNX4                           1272 transport         lipid binding           D, the      membraneSNX4                     0
                      Sorting nexin, involved in the retrieval of late-Golgi SNAREs fromC, A post-Golgi endosome to the trans-Golgi network and in cyto
YKR031C     SPO14                          5052 sporulation       phospholipase D activityD           endosomeSPO14                    0
                      Phospholipase D, catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-i
YLR119W     SRN2      Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the 0
                                            642 protein targeting to vacuole
                                                                  unknown                 D                       SRN2
                                                                                                      endosome, pre-vacuole             endosome; suppressor
YAR042W     SWH1                           3567 steroid biosynthesis
                                                                  phosphatidylinositol bindingD, EER
                                                                                          N, G,                   SWH1                 0
                      Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole
YDR468C     TLG1                            675 vesicle fusion with Tlg2p and Vti1p G, D mediates fusion TLG1
                      Essential t-SNARE that forms a complexv-SNARE activity              and         Golgi       of endosome-derived 1 vesicles with the late G
YPL045W     VPS16                          2397 protein fusion unknown
                                                                  and vacuole             V, D        vacuole VPS16                    0
                      Subunit of the homotypic vacuole targeting to vacuole protein sorting (HOPS) complex; part of the Class C Vps complex essentia
YKL002W     VPS2      Class E Vps protein of theprotein retention in Golgi
                                            767 ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multive
                                                                  protein binding         D, C                     endosome
                                                                                                      cytoplasm,VPS2                   0
YHR012W     VPS29                           968 a subunit transport, endosome to Golgi
                                                                  unknown                 D           endosomeVPS29                    0
                      Endosomal protein that is retrogradeof the membrane-associated retromer complex essential for endosome-to-Golgi retrograde tr
YDR495C     VPS3                           required for targeting and processing of D, C
                                                                  unknown
                      Cytoplasmic protein 3036 proteinthe sortingto vacuole                           cytoplasm VPS3                   0
                                                                                          soluble vacuolar proteins, acidification of the vacuolar lumen, and
YLR396C     VPS33                          that Endosome late to vacuole transport, fusion
                                                                  ATP binding              HOPS vacuole, protein sorting (HOPS)0complex; essential for
                                                                                                                  VPS33
                      ATP-binding protein 2076is a subunit of the homotypic vacuoleV, D, C and vacuolegolgi, endosome
YLR240W     VPS34                          3-kinase responsible for thekinase activityV, D, C, A Golgi, membrane
                                                                  protein synthesis of                            VPS34                0
                      Phosphatidylinositol2628 protein amino acid phosphorylation phosphatidylinositol 3-phosphate; forms membrane-associated sig
YJL154C     VPS35                          2835 protein retention in retromer complex required endosomeVPS35
                                                                  unknown
                      Endosomal subunit of membrane-associated Golgi                      D                                            0
                                                                                                      for retrograde transport; receptor that recognizes retriev
YPR173C     VPS4                           1314 protein retention endosome to vacuoleE, C cytoplasm,VPS4 release of an endosomal membrane-a
                                                                  ATPase activity
                      AAA-type ATPase required for efficient latein Golgi                                          pre-VACUOLE
                                                                                          D, transport; catalyzes the                  0
YOL129W     VPS68                           555 protein targeting to vacuole
                                                                  function                M, V, D Mito            VPS68
                      Vacuolar membrane protein of unknown unknowninvolved in vacuolar protein sorting; also detected in the4           mitochondria
YLR181C     VTA1                            993 protein involved vacuole transport D
                                                                  unknown                              binds to Vps20p                 0
                      Multivesicular body (MVB)Endosome late toin endosomal protein sorting; endosomeVTA1 and Vps4p; may regulate Vps4p functi
YIL173W     VTH1                           glycoprotein with strong similarity
                                                                  signal sequence binding D           endosomeVTH1                     1
                      Putative membrane 4650 Golgi to vacuole transport to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting
YGR198W     YGR198W                        2454 MAPKKK cascade    transferase interact P, D, C, Rcytoplasm YGR198W                     0
                      Cargo-transport protein involved in endocytosis; mayactivity with ribosomes, based on co-purification experiments; GFP-fusion
YCR088W     ABP1                           1779 establishment protein binding
                                                                  cytoskeleton,           C           cytoplasm ABP1
                      Actin-binding protein of the cortical actin of cell polarity important for activation of the Arp2/3 complex that 0plays a key role actin in
YOR239W     ABP140                         1888 actin actin filaments and localizes to actin patches filament
                                                                  S-adenosylmethionine-dependent methyltransferase activity
                                                                                          C                       ABP140
                      Nonessential protein that binds cytoskeleton organization and biogenesisactinand cables, has similarity to0       S-adenosylmethionine
YNL220W     ADE12                          1302 purine nucleotide biosynthesis synthase 'de cytoplasm ADE12
                                                                  adenylosuccinate         in         novo'
                      Adenylosuccinate synthase, catalyzes the first committed stepC theactivity biosynthesis of adenosine 0
YLR028C     ADE16     Enzyme of 'de novo'1776 aerobic respiration cyclohydrolase activity
                                            purine biosynthesis IMP
                                                                  containing both 5-aminoimidazole-4-carboxamide ribonucleotide0
                                                                                          C           cytosol     ADE16                 transformylase and ino
YOR128C     ADE2                           1716 de novo' IMP biosynthesis
                                                                  phosphoribosylaminoimidazole carboxylase activity
                                                                                          C           cytoplasm ADE2                   0
                      Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigmen
YMR300C     ADE4                           1533 de novo' IMP biosynthesis
                                                                  amidophosphoribosyltransferase activity ADE4
                                                                                          C           cytoplasm                        first
                      Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes 0 step of the 'de novo
YGL234W     ADE5,7                          of the 'de novo' purine nucleotide
                                                                  phosphoribosylamine-glycine ligase activity
                                                                                          C           cytoplasm ADE5,7                 0
                      Bifunctional enzyme2409 de novo' IMP biosynthesis biosynthetic pathway, contains aminoimidazole ribotide synthetase and gly
YGR061C     ADE6                           4077 de novo' (FGAM)-synthetase, catalyzes a step in the 'deADE6 purine nucleotide biosynthetic pathway
                                                                  phosphoribosylformylglycinamidine synthase activity
                      Formylglycinamidine-ribonucleotideIMP biosynthesis                  C           cytoplasm novo'                  0
YMR318C     ADH6                           1083 aldehyde metabolism dehydrogenase (NADP+) activity ADH6
                                                                  alcohol                 C           soluble fraction                 0
                      NADPH-dependent cinnamyl alcohol dehydrogenase family member with broad substrate specificity; may be involved in fusel alc
YDR524C     AGE1                           1449 ER GTPase activating
                                                                  ARF GTPase activator effector, involved in the secretory and endocytic pathways; conta
                                                                                          C
                      ADP-ribosylation factor (ARF)to Golgi transport protein (GAP) activity cytoplasm AGE1                            0
YIL044C     AGE2                            897 ER GTPase activating
                                                                  ARF GTPase activator effector, involved inAGE2
                                                                                          C                        Trans-Golgi-Network0
                      ADP-ribosylation factor (ARF)to Golgi transport protein (GAP) activity clathrin-coated vesicle                    (TGN) transport; conta
YDR214W     AHA1                           1053 response and chaperone activator activity similar to Hch1p; expression is regulated by stresses such
                      Co-chaperone that binds to Hsp82p to stress                         C
                                                                  activates its ATPase activity; cytoplasm AHA1                        0
YBR059C     AKL1                           3327 actin cytoskeleton organization and biogenesiscytoplasm AKL1
                                                                  protein Prk1p) of the Ark                                            0
                      Ser-Thr protein kinase, member (with Ark1p andkinase activityC kinase family; involved in endocytosis and actin cytoskeleton o
YMR170C     ALD2                           1521 aldehyde metabolism
                                                                  aldehyde ethanol oxidation and cytoplasm ALD2
                                                                                          C
                      Cytoplasmic aldehyde dehydrogenase, involved in dehydrogenase activity           beta-alanine biosynthesis; uses 0NAD+ as the preferred
YML035C     AMD1                           2433 purine nucleotide metabolismdeamination of AMP to formAMD1
                                                                    catalyzes the
                      AMP deaminase, tetrameric enzyme thatAMP deaminase activity         C                                            0
                                                                                                      cytoplasm IMP and ammonia; may be involved in regul
YNL094W     APP1      Protein of unknown function, interacts with Rvs161p and Rvs167p; computational analysis of protein-protein interactions in large-
                                           1764 actin filament organization
                                                                  unknown                 C                       APP1
                                                                                                      actin cortical patch             0
YJL024C     APS3      Small subunit of the clathrin-associated adaptor complex AP-3, which is involved inAPS3
                                            662 vesicle-mediated transport
                                                                  unknown                 C                       vacuolar             0
                                                                                                      Golgi, endosome? protein sorting; related to the sigma
YBR149W     ARA1      Large subunit of NADP+ dependent arabinose dehydrogenase, involved cytosol
                                           1035 carbohydrate metabolism
                                                                  aldo-keto reductase activity
                                                                                          C                       ARA1                 0
                                                                                                      in carbohydrate metabolism; small subunit is unidentifie
YGL105W     ARC1                           1131 tRNA-nucleus exportbinding
                                                                  tRNA                    C           cytoplasm ARC1                   0
                      Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulatin
YNR035C     ARC35                          1029 actin cytoskeleton organization and biogenesiscytosol
                                                                    required molecule activity
                                                                                          C                       ARC35                0
                      Subunit of the ARP2/3 complex, which isstructural for the motility and integrity of cortical actin patches; required for cortical locali
YOL058W     ARG1      Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynt
                                           1263 arginine biosynthesis
                                                                  argininosuccinate synthase activity
                                                                                          C           cytosol     ARG1                 0
YDR127W     ARO1                           4767 aromatic amino acid family biosynthesis
                                                                  3-dehydroquinate dehydratase activity
                                                                                          C           cytoplasm ARO1                   0
                      Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic am
YGL148W     ARO2                           1131 aromatic amino acid family biosynthesis
                                                                  chorismate synthase C   activity    cytoplasm 5-enolpyruvylshikimate 3-phosphate (EPSP)
                      Bifunctional chorismate synthase and flavin reductase, catalyzes the conversion of ARO2                          0
YGL202W     ARO8                           1503 aromatic amino regulated by general control ofcytoplasm ARO8
                                                                  aromatic-amino-acid transaminase activity biosynthesis
                      Aromatic aminotransferase, expression is acid family metabolism     C            amino acid                      0
YPR145W     ASN1                           1719 asparagine biosynthesis the synthesis of L-asparagineASN1L-aspartate in the asparagine biosynthe
                                                                  asparagine
                      Asparagine synthetase, isozyme of Asn2p; catalyzessynthase C                    cytoplasm from
                                                                                          (glutamine-hydrolyzing) activity             0
YGR124W     ASN2                           1719 asparagine biosynthesis the synthesis of L-asparagineASN2L-aspartate in the asparagine biosynthe
                                                                  asparagine
                      Asparagine synthetase, isozyme of Asn1p; catalyzessynthase C                    cytoplasm from
                                                                                          (glutamine-hydrolyzing) activity             0
YDR321W     ASP1                           1146 involved in asparagine
                                                                  asparaginase activityC
                      Cytosolic L-asparaginase,asparagine catabolism catabolism                       cytosol     ASP1                 0
YJL020C     BBC1      Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains
                                           3474 actin cytoskeleton organization and biogenesis
                                                                  myosin I binding        C                       BBC1
                                                                                                            cortical patch             0
YPL255W   BBP1     Protein required for the spindle pole body (SPB) duplication, localized at spindle pole body periphery; forms a complex with a nu
                                         1158 microtubule nucleation constituent C cytoskeleton
                                                                structural              of                       plaque
                                                                                                    the centralBBP1                   0
YAL060W   BDH1                           1149 butanediol fermentation
                                                                (R,R)-butanediol dehydrogenase medium-chain
                                                                                        C          cytoplasm                          0
                   NAD-dependent (2R,3R)-2,3-butanediol dehydrogenase, a zinc-containingactivity BDH1 alcohol dehydrogenase, produces 2,3-b
YJR053W   BFA1     Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple polecycle checkpoint pathways that control exit f
                                         1725 conjugation with cellularactivator activity
                                                                GTPase fusion           C                      BFA1
                                                                                                   spindle cell body                  0
YER016W   BIM1     Microtubule-binding 1035 microtubule nucleation constituent C cytoskeleton pole body
                                          protein that together structural makes upof cortical microtubule capture site and delays the exit from mito
                                                                 with Kar9p              the       spindle     BIM1                   0
YJR025C   BNA1                             534 NAD biosynthesis 3-hydroxyanthranilateC   3,4-dioxygenase from tryptophan via kynurenine pathway
                                                                                                   cytoplasm BNA1
                   3-hydroxyanthranilic acid dioxygenase, required for biosynthesis of nicotinic acidactivity                         0
YNL166C   BNI5                           1347 cytokinesis       unknown                 C          bud neck BNI5                      0
                   Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud
YDL141W   BPL1                           2073 protein amino acid biotinylation
                                                                biotin-[acetyl-CoA-carboxylase] ligase activity
                                                                                        C           biotin, required                  0
                   Biotin:apoprotein ligase, covalently modifies proteins with the addition of cytoplasm BPL1for acetyl-CoA carboxylase (Acc1p) holo
YNR051C   BRE5                           1548 protein deubiquitination
                                                                unknown                 C          cytoplasm BRE5
                   Ubiquitin protease cofactor, formsdeubiquitination complex with Ubp3p that coregulates anterograde and0retrograde transport be
YMR055C   BUB2     Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks
                                           921 mitotic spindle GTPase activator activity
                                                                 checkpoint             C                      BUB2
                                                                                                   spindle pole body                  1
YCL014W   BUD3                           4911 cytokinesis         required              C          bud neck BUD3                      0
                   Protein involved in bud-site selection andunknown for axial budding pattern; localizes with septins to bud neck in mitosis and may
YLR319C   BUD6                           2367 actin filament organization cable nucleation binding
                                                                cytoskeletal            C          actin cap BUD6                     as
                   Actin- and formin-interacting protein, involved in actinregulatory protein and polarized cell growth; isolated0 bipolar budding mut
YML111W   BUL2                           2763 protein monoubiquitination
                                                                unknown                 C          cytoplasm BUL2                     0
                   Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat sho
YHR114W   BZZ1                            implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its
                                                                unknown
                   SH3 domain protein1902 actin filament organization                   C          cytoplasm BZZ1                     0
YKL007W   CAP1                             807 barbed-end actin filament capping
                                                                actin filament bindingC            actin cortical patch
                                                                                                               CAP1                   0
                   Alpha subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments prev
YIL034C   CAP2                             864 filamentous growth
                                                                heterodimer (Cap1p and             actin cortical to the
                                                                                                               CAP2
                   Beta subunit of the capping protein (CP) actin filament bindingC Cap2p) which bindspatch barbed ends0of actin filaments preve
YIL142W   CCT2                           1584 protein folding Cct ring protein binding  C          cytoplasm CCT2
                   Subunit beta of the cytosolic chaperonin unfoldedcomplex, related to Tcp1p, required for the assembly of 0         actin and tubulins in viv
YJL014W   CCT3                           1605 protein folding unfolded protein binding  C          cytoplasm the assembly of actin0
                   Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required forCCT3                            and tubulins in vivo
YDL143W   CCT4                           1587 protein folding unfolded protein binding  C          cytoplasm the assembly of actin0
                   Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required forCCT4                            and tubulins in vivo
YJR064W   CCT5                           1689 protein folding unfolded protein binding  C          cytoplasm the assembly of actin0
                   Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required forCCT5                            and tubulins in vivo
YDR188W   CCT6                           1641 protein folding unfolded protein binding  C          cytoplasm CCT6                     0
                   Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin
YJL111W   CCT7                           1653 protein folding unfolded protein binding  C          cytoplasm the assembly of actin0
                   Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required forCCT7                            and tubulins in vivo
YJL008C   CCT8                           1707 protein folding unfolded protein binding  C          cytoplasm the assembly of actin0
                   Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required forCCT8                            and tubulins in vivo
YDR168W   CDC37                          1521 signal necessary for protein binding
                                                                unfolded                C
                   Essential Hsp90p co-chaperone;transduction passage through the START phase CDC37cytoplasm of the cell cycle        0
YPL160W   CDC60                          3273 leucyl-tRNA aminoacylation appropriate
                                                                  leucine to the        C
                   Cytosolic leucyl tRNA synthetase, ligatesleucine-tRNA ligase activitytRNA       cytoplasm CDC60                    0
YGL206C   CHC1                           4962 endocytosis structural molecule activity  C          clathrin vesicle coat
                                                                                                               CHC1                   0
                   Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chain
YMR198W   CIK1                           1785 meiosis
                   Kinesin-associated protein required for both karyogamy andactivity spindle organization, interacts stably and specifically with Ka
                                                                microtubule motor mitotic
                                                                                        C                      CIK1
                                                                                                   spindle pole body                  0
YNL298W   CLA4                           2529 protein amino acid phosphorylation C kinase activity
                                                                protein serine/threonine           actin family, involved in septin ring assembly and cytoki
                   Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase)cap CLA4                               0
YGR167W   CLC1                             702 endocytosis structural molecule activity C          clathrin vesicle coat and endocytosis; thought to regulat
                   Clathrin light chain, subunit of the major coat protein involved in intracellular proteinCLC1 transport            0
YMR012W   CLU1                           3834 translational initiation
                                                                unknown                  in        cytoplasm CLU1                     0
                   eIF3 component of unknown function; deletion causes defectsC mitochondrial organization but not in growth or translation initiat
YBR109C   CMD1                             444 protein that protein Ca++ independent processes (mitosis, bud growth, actin0
                   Calmodulin; Ca++ bindingendocytosis regulates binding                C          cytoplasm CMD1                      organization, endocyto
YOL016C   CMK2                           1344 protein amino acid phosphorylation C response, many CA++/calmodulan
                                                                calcium- role in stress            cytoplasm CMK2                     0
                   Calmodulin-dependent protein kinase; may play a and calmodulin-dependent protein kinase activity dependent phosphorylation s
YML057W   CMP2                           1815 cell ion homeostasis
                                                                calcium-dependent protein serine/threonine phosphatase activity0
                   Calcineurin A; one isoform (the other is CNA1) of the catalytic C                calcineurin, a Ca++/calmodulin-regulated protein phosp
                                                                                        subunit of cytoplasm CMP2
YLR433C   CNA1                           1662 cell wall organization of the catalyticC
                                                                calcium-dependent protein serine/threonine phosphatase activity0
                                                                                                     calcineurin, a Ca++/calmodulin-regulated protein phosp
                   Calcineurin A; one isoform (the other is CMP2) and biogenesissubunit ofcytoplasm CNA1
YKL190W   CNB1                             604 cell wall organization and biogenesis
                                                                calcium ion Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a s
                   Calcineurin B; the regulatory subunit of calcineurin, abinding C                cytoplasm CNB1                     0
YNL225C   CNM67    Component of the spindle microtubule nucleation constituent C cytoskeleton pole body
                                         1746 pole body outer plaque; required forof
                                                                structural                         spindle     CNM67                  0
                                                                                         spindle orientation and mitotic nuclear migration
YBR155W   CNS1                           1158 protein folding unfolded (Hsp90) and Ssa1p (Hsp70) andCNS1
                   TPR-containing co-chaperone; binds both Hsp82pprotein binding        C                                             0
                                                                                                   cytoplasm stimulates the ATPase activity of SSA1, ts m
YLR216C   CPR6                           1116 protein folding unfolded protein the C
                   Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzesbinding             cytoplasm CPR6                     0
                                                                                        cis-trans isomerization of peptide bonds N-terminal to proline resid
YLR429W   CRN1     Coronin, cortical actin cytoskeletal component that associates C
                                         1956 actin filament organization
                                                                protein binding, bridging the Arp2p/Arp3p complex to regulate its activity
                                                                                         with      actin cortical patch
                                                                                                               CRN1                   0
YDR371W   CTS2                            chitinase
                   Sporulation-specific 1536 unknown            unknown                 C          cytoplasm CTS2                     1
YGR088W   CTT1                           1722 response to stresscatalase oxidative C               cytoplasm CTT1
                   Cytosolic catalase T, has a role in protection from activity damage by hydrogen peroxide                           0
YOR042W   CUE5                            CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent p
                   Protein containing a1236 unknown             unknown                 C          cytoplasm CUE5                     0
YML070W   DAK1                           1755 required to detoxification of dihydroxyacetonecytoplasm DAK1 in stress adaptation
                   Dihydroxyacetone kinase, responsefor stress                          C
                                                                glycerone kinase activity            (DHA); involved                  0
YIR031C   DAL7                           1665 allantoin catabolism synthase activity
                                                                malate                  C          cytoplasm DAL7                     0
                   Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by all
YPL119C   DBP1                           1854 translational of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal sub
                   Putative ATP-dependent RNA helicaseinitiationRNA helicase activityC             cytoplasm DBP1                     0
YHR144C   DCD1                             939 pyrimidine nucleotide deaminase activity
                                                                dCMP metabolism C                  cytoplasm DCD1
                   Deoxycytidine monophosphate (dCMP) deaminase required for dCTP and dTTP synthesis; expression is 0                 NOT cell cycle regulate
YHR019C   DED81                          1665 asparaginyl-tRNA aminoacylation C
                                                                ATP binding                        cytoplasm DED81                    0
                   Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cog
YIR004W   DJP1                           1299 Pero matrix required for peroxisomal
                                                                chaperone                          cytosol     DJP1                   0
                   Cytosolic J-domain-containing protein,protein importbinding C protein import and involved in peroxisome assembly, homologou
YEL071W   DLD3                           1491 part of the retrograde dehydrogenase
                                                                D-lactate                consists of genes whose
                                                                                                   cytoplasm DLD3                     0
                   D-lactate dehydrogenase, lactate metabolism regulon whichC (cytochrome) activity expression is stimulated by damage to m
YHR115C   DMA1                           1251 mitotic spindle unknown
                                                                 checkpoint
                   Protein involved in regulating spindle position and orientation, C              cytoplasm DMA1                     0
                                                                                        functionally redundant with Dma2p; homolog of S. pombe Dma1 a
YNL116W   DMA2                           1569 mitotic spindle unknown
                                                                 checkpoint
                   Protein involved in regulating spindle position and orientation, C              cytoplasm DMA2                     0
                                                                                        functionally redundant with Dma1p; homolog of S. pombe Dma1 a
YNL001W   DOM34                          1161 protein biosynthesis
                                                                in protein              C            G1 progression and               0
                   Probable RNA-binding protein, functions unknown translation to promotecytoplasm DOM34 differentiation, required for meiotic
YDR068W   DOS2                             933 unknown          unknown                 C          cytoplasm DOS2
                   Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 0
YIL103W   DPH1                           1278 peptidyl-diphthamide biosynthesis from peptidyl-histidine DPH1
                                                                unknown                 C          cytoplasm                          0
                   Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue
YKL191W   DPH2                           1605 peptidyl-diphthamide biosynthesis from peptidyl-histidine DPH2
                                                                unknown                 C          cytoplasm                          0
                   Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue
YPR017C   DSS4                             432 secretory pathwayguanyl-nucleotide exchange factor activity
                                                                                        C          membraneDSS4                       0
                   Nucleotide release factor functioning in the post-Golgi secretory pathway, required for ER-to-Golgi transport, binds zinc, found bo
YDL219W   DTD1                             524 translation      protein translation, may affect nonsense DTD1
                                                                                        C          cytoplasm                          0
                   D-Tyr-tRNA(Tyr) deacylase, functions in hydrolase activity, acting on ester bonds suppression via alteration of the protein synthe
YBR208C   DUR1,2                         5508 allantoin catabolism
                                                                allophanate hydrolase activity cytoplasm DUR1,2
                                                                                        C                                             0
                   Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; express
YKR054C   DYN1                           chain mitotic microtubule motor protein, required for cytoplasmic microtubule
                                                                motor activity
                   Cytoplasmic heavy12279 dynein, sister chromatid segregation C                                spindle               0
                                                                                                    anaphase DYN1 elongation; involved in spindle assem
YHR068W   DYS1                           1164 catalyzes formation of deoxyhypusine, the
                                                                transferase activity, transferring alkyl or aryl (other than methyl) groups
                                                                                        C            step in hypusine                 0
                   Deoxyhypusine synthase, hypusine biosynthesis from peptidyl-lysine firstcytoplasm DYS1 biosynthesis; triggers posttranslationa
YJR137C   ECM17                         4329 cell involved in amino acid biosynthesis, activity
                                                               sulfite and biogenesis   C           cytoplasm ECM17
                   Sulfite reductase beta subunit,wall organization reductase (NADPH) transcription repressed by methionine           0
YJL201W   ECM25                         1800 cell wall organization and biogenesis
                                                               unknown                  a           cytoplasm sequence
                   Non-essential protein of unknown function; promoter contains C consensus binding ECM25 for factor Abf1p            0
YBL047C   EDE1                          4146 endocytosis unknown                        C           actin cortical patch, bud
                                                                                                                EDE1                  0
                   Key endocytic protein involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-depend
YHR193C   EGD2     Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) polypeptide-associated complex
                                          525 nascent polypeptide association
                                                               unfolded protein binding C                       EGD2                  0
                                                                                                    nascent involved in protein sorting and translocation, as
YDR516C   EMI2                          1503 sporulation       unknown                  C           cytoplasm EMI2                    0
                   Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IM
YNL084C   END3                          1050 endocytosis
                   EH domain-containing protein involved inprotein binding, bridging
                                                                 endocytosis, actin cytoskeletal actin cortical patch wall morphogenesis; forms a comp
                                                                                        C            organization and cell
                                                                                                                END3                  0
YDL161W   ENT1                          1365 in endocytosis and actin patch             C           functionally redundant with Ent2p; binds clathrin via a cla
                   Epsin-like protein involvedendocytosis clathrin binding assembly and actin cortical patch    ENT1                  0
YLR206W   ENT2                          1842 for endocytosis and actin patch            C            functionally patch
                                                                                                                ENT2                  0
                   Epsin-like protein requiredendocytosis clathrin binding assembly andactin cortical redundant with Ent1p; contains clathrin-bind
YJR125C   ENT3                          1227 endocytosis protein binding
                   Protein containing an N-terminal epsin-like domain involved in C                 actin cortical patch
                                                                                                                ENT3
                                                                                         clathrin recruitment and traffic between the0Golgi and endosomes;
YPL028W   ERG10    Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA
                                        1197 ergosterol biosynthesis C-acetyltransferase cytosol
                                                               acetyl-CoA               C           activity    ERG10                 0
YMR208W   ERG12    Mevalonate kinase, 1332in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
                                        acts ergosterol biosynthesis
                                                               mevalonate kinase activity
                                                                                        C           cytosol     ERG12                 0
YMR220W   ERG8     Phosphomevalonate kinase, an essential cytosolic enzyme that acts activity
                                        1356 ergosterol biosynthesis
                                                               phosphomevalonate kinase in the biosynthesis of isoprenoids and sterols, including erg
                                                                                        C           cytosol     ERG8                  0
YBR102C   EXO84                         2262 roles in spliceosome assembly andC
                   Essential protein with dualexocytosis       protein binding                      bud neck EXO84                    0
                                                                                         exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, S
YDL045C   FAD1                            921 FAD biosynthesis performs the second stepcytoplasm FAD1
                                                               FMN                      C
                   Flavin adenine dinucleotide (FAD) synthetase,adenylyltransferase activityin synthesis of FAD from riboflavin       0
YLR377C   FBP1                          1047 gluconeogenesis   fructose-bisphosphatase activitycytosol
                                                                                        C                       FBP1
                   Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism 0
YLR060W   FRS1                          1788 phenylalanyl-tRNA aminoacylation ligase tetramer
                                                               phenylalanine-tRNA C                 cytoplasm FRS1                    0
                   Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a activity with Frs2p to generate active enzyme; sequence is
YFL022C   FRS2                          1512 phenylalanyl-tRNA aminoacylation ligase activity
                                                               phenylalanine-tRNA C                 cytoplasm FRS2                    0
                   Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily di
YMR222C   FSH2                             localizes to the    serine hydrolase activityC           cytoplasm FSH2
                   Serine hydrolase that672 unknown cytoplasm; sequence is similar to Fsh1p and Fsh3p                                 0
YOR280C   FSH3                            801 unknown          Fsh1p and Fsh2p
                   Serine hydrolase; sequence is similar to serine hydrolase activity   C           cytoplasm FSH3                    0
YOR260W   GCD1                          1737 translational initiation
                                                               translation initiation factor activity
                                                                                        C           ribosome GCD1
                   Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2;0activity subsequently r
YDR211W   GCD6                          2139 translational initiation
                                                               translation initiation factor activity
                                                                                        C           ribosome GCD6                     for
                   Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor 0 eIF2; activity subseq
YKR026C   GCN3     Alpha subunit of the translation initiation translation initiation factor activity
                                          918 translational initiationeIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently reg
                                                               factor                   C                        initiation
                                                                                                    translation GCN3 factor 2B complex0
YER136W   GDI1                          1356 vesicle-mediated traffic in
                                                               Rab GDP-dissociation inhibitor activity
                                                                                        C            by regulating the
                   GDP dissociation inhibitor, regulates vesicletransport secretory pathwaysmembraneGDI1 dissociation of0GDP from the Sec4/Yp
YNL153C   GIM3                            390 tubulin folding tubulin binding           C           cytoplasm GIM3                    0
                   Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers tar
YEL003W   GIM4                            372 tubulin folding tubulin binding           C           cytoplasm GIM4                    1
                   Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers tar
YML094W   GIM5                            575 tubulin folding tubulin binding           C           cytoplasm GIM5                    0
                   Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers tar
YDR152W   GIR2                            798 unknown          unknown                  C           cytoplasm GIR2                    0
                   Highly-acidic cytoplasmic RWD domain-containing protein of unknown function, sensitive to proteolysis, N-terminal region has hi
YNL255C   GIS2                            462 intracellular signaling cascade
                                                               TF activity              C           cytoplasm GIS2                    0
                   Protein with seven cysteine-rich CCHC zinc-finger motifs, similar to human CNBP, proposed to be involved in the RAS/cAMP sig
YOR168W   GLN4                          2430 glutaminyl-tRNA aminoacylation
                                                                class I tRNA synthetase that catalyzes the specific
                                                                                        C                                             0
                   Glutamine tRNA synthetase, monomeric glutamine-tRNA ligase activity cytoplasm GLN4 glutaminylation of tRNA(Glu); N-term
YDR272W   GLO2                            825 catalyzes the hydrolysis
                                                               hydroxyacylglutathione hydrolase activity GLO2
                                                                                        C           cytoplasm
                   Cytoplasmic glyoxalase II,carbohydrate metabolismof S-D-lactoylglutathione into glutathione and D-lactate          0
YEL046C   GLY1     Threonine aldolase, 1164 threonine catabolismL-allo-threonineC
                                         catalyzes the cleavage of
                                                               threonine aldolase activity L-threonine to glycine; involved in glycine biosynthesis
                                                                                         and        cytosol     GLY1                  0
YGR256W   GND2     6-phosphogluconate1479 glucose metabolism
                                          dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the0
                                                               phosphogluconate dehydrogenase (decarboxylating) activity
                                                                                        C           cytosol     GND2                   pentose phosphate pat
YPR160W   GPH1                          2709 glycogen catabolism
                                                               glycogen phosphorylase activitycytoplasm GPH1
                                                                                        C                                             0
                   Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated pho
YLR258W   GSY2                           similar to Gsy1p; expression (starch) synthase activity
                                                               glycogen                 C           cytoplasm GSY2                    0
                   Glycogen synthase, 2118 glycogen biosynthesis induced by glucose limitation, nitrogen starvation, heat shock, and stationary ph
YGL245W   GUS1                          2127 glutamyl-tRNA glutamate-tRNA ligase activity cytoplasm GUS1
                                                                aminoacylation          C                                             0
                   Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation in
YKR084C   HBS1                           with protein biosynthesis
                                                               unknown
                   GTP binding protein1836 sequence similarity to the elongationC                   cytoplasm HBS1                    0
                                                                                         factor class of G proteins, EF-1alpha and Sup35p; associates with
YLR192C   HCR1     Dual function protein 798 proteintranslation initiationinitiation factor activity component of eukaryotic translation initiation factor 3
                                          involved in biosynthesis
                                                               translation as a substoichiometric
                                                                                        C                        initiation
                                                                                                    translation HCR1 factor 2B complex0
YGR187C   HGH1                          1185 unknown           unknown                  C           cytoplasm the cytoplasm
                   Protein of unknown function with similarity to human HMG1 and HMG2; localizes toHGH1                               0
YER052C   HOM3                          1584 methionine metabolism kinase activity
                                                               aspartate                C            catalyzes the first              0
                   Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme thatcytoplasm HOM3 step in the common pathway for met
YOR267C   HRK1                          2280 cell ion homeostasis
                                                                the plasma membrane                 cytoplasm HRK1                    0
                   Protein kinase implicated in activation of protein kinase activityC H(+)-ATPase Pma1p in response to glucose metabolism; plays a
YBR272C   HSM3                          1443 mismatch repair   unknown
                   Protein of unknown function, involved in DNA mismatch repairC                    cytoplasm HSM3                    0
                                                                                         during slow growth; has weak similarity to Msh1p
YDR533C   HSP31                           714 unknown
                   Possible chaperone and cysteine protease with similarity to E.C Hsp31 and S.fraction
                                                               unfolded protein binding  coli                   HSP31                 0
                                                                                                    soluble cerevisiae Hsp32p, Hsp33p, and Sno4p; memb
YPL015C   HST2                          1074 chromatin silencing
                                                               NAD-dependent histone deacetylase activity
                                                                                        C           cytoplasm HST2                    0
                   Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates
YPL117C   IDI1     Isopentenyl diphosphate:dimethylallyl diphosphate isomerase C delta-isomerase activity an essential activation step in the isopr
                                          867 ergosterol biosynthesis
                                                               isopentenyl-diphosphate isomerase), catalyzes
                                                                                        (IPP        cytosol     IDI1                  0
YBL076C   ILS1                          3219 protein biosynthesis
                                                               isoleucine-tRNA ligase activity cytosol
                                                                                        C
                   Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A    ILS1                  0
YHR216W   IMD2                          1572 GTP biosynthesis    catalyzes the first step of GMP cytoplasm IMD2
                   Inosine monophosphate dehydrogenase,IMP dehydrogenase activity       C                                             0
                                                                                                     biosynthesis, expression is induced by mycophenolic ac
YML056C   IMD4                          1983 unknown             catalyzes the first step of GMP cytoplasm IMD4
                   Inosine monophosphate dehydrogenase,IMP dehydrogenase activity       C                                             0
                                                                                                     biosynthesis, member of a four-gene family in S. cerevi
YOR109W   INP53                          4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an
                                                               inositol-polyphosphate   C           membraneINP53                     0
                   Phosphatidylinositol3324 cell wall organization and biogenesis 5-phosphatase activity N-terminal Sac1 domain, plays a role in a
YMR132C   JLP2                            627 unknown          unknown                  C           cytoplasm (typified by the E. coli0DnaJ protein)
                   Protein of unknown function, contains sequence that closely resembles a J domain JLP2
YBR017C   KAP104   Transportin, cytosolic karyopherin beta 2nuclear localization sequence binding nuclear ribonucleoproteins to the nucleoplasm, bi
                                        2757 protein-nucleusinvolved in delivery of heterogeneous
                                                                 import                 C           cytosol     KAP104                0
YPR141C   KAR3                          2190 meiosis
                   Minus-end-directed microtubule motor that functions in mitosisC
                                                               microtubule motor activity meiosis, localizesbody spindle pole body and localization is
                                                                                          and       spindle pole to the
                                                                                                                KAR3                  0
YDR017C   KCS1                          3153 response to stressinositol or phosphatidylinositol kinase activity
                                                                                        C           cytoplasm KCS1                    0
                   Inositol hexaphosphate kinase, phosphorylates inositol hexakisphosphate (InsP6) to diphosphoinositol polyphosphates, required
YHR158C   KEL1                          3495 cellular morphogenesis
                                                               unknown                  C            complex KEL1                     0
                   Protein required for proper cell fusion and cell morphology; functions in acytoplasmwith Kel2p to negatively regulate mitotic exit, in
YPL263C   KEL3                           of unknown functionunknown
                   Cytoplasmic protein 1956 unknown                                     C           cytoplasm KEL3                    0
YOR233W   KIN4                          2403 unknown           protein kinase activityC             cytoplasm KIN4                    0
                   Kinase that acts by inhibiting the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymme
YBL063W   KIP1     Kinesin-related motor protein required nucleation spindle assembly and chromosome body
                                        3336 microtubule for mitotic constituent C cytoskeleton pole segregation; functionally redundant with Cin
                                                               structural               of          spindle     KIP1                  0
YDR037W   KRS1                          1776 lysyl-tRNA aminoacylation ligase activity
                                                                 a negative             of           control of KRS1
                   Lysyl-tRNA synthetase; also identified aslysine-tRNA regulator C general cytoplasm amino acid biosynthesis         0
YOR181W   LAS17                         1902 endocytosis cytoskeletal protein binding   C           cytoplasm LAS17                   0
                   Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 co
YLR260W   LCB5                          2064 response to heat   paralog of Lcb4p responsible activity
                                                                                        C           membraneLCB5
                   Minor sphingoid long-chain base kinase, D-erythro-sphingosine kinase for few percent of the total activity,0        possibly involved in syn
YOR322C   LDB19                          2457 unknown          unknown                 C           length; null mutant                  0
                   Protein of unknown function involved in maintenance of proper telomere cytoplasm LDB19 shows a reduced affinity for the alcia
YGL009C   LEU1     Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway
                                         2340 leucine biosynthesis
                                                               3-isopropylmalate dehydratase activity
                                                                                       C           cytosol     LEU1                     0
YCL018W   LEU2                           1095 leucine biosynthesis
                                                               3-isopropylmalate dehydrogenase activity LEU2
                                                                                        in         cytosol
                   Beta-isopropylmalate dehydrogenase, catalyzes the third step C the leucine biosynthesis pathway                      0
YCL034W   LSB5                           1065 endocytosis unknown                      C           cell cortex LSB5                     0
                   Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patc
YGL099W   LSG1                           1923 in 60S ribosomal subunit biogenesis; requiredcytoplasm LSG1 Nmd3p from 60S subunits in the cytop
                   Putative GTPase involvedsporulation         GTPase activity         C            for the release of                  0
YBR115C   LYS2                           reductase, biosynthesis reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is t
                                                               L-aminoadipate-semialdehyde dehydrogenase activity
                   Alpha aminoadipate4179 lysinecatalyzes the via aminoadipic acid     C           cytoplasm LYS2                       0
YNR050C   LYS9                           1341 lysine biosynthesis via aminoadipic C
                                                               saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
                   Saccharopine dehydrogenase (NADP+, L-glutamate-forming);acid                    the formation of                     0
                                                                                        catalyzes cytoplasm LYS9 saccharopine from alpha-aminoadipate
YBL091C   MAP2                           1266 proteolysis      methionyl aminopeptidase activity
                                                                                       C           cytoplasm MAP2
                   Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent0polypeptides; function
YNL307C   MCK1                           1128 protein amino acid phosphorylation C
                                                               glycogen synthase kinase 3 activity soluble fraction
                                                                                                               MCK1                     0
                   Protein serine/threonine/tyrosine (dual-specificity) kinase involved in control of chromosome segregation and in regulating entry i
YOL076W   MDM20                          2391 mitochondrial organization and biogenesis
                                                               peptide alpha-N-acetyltransferase activity MDM20
                                                                                       C           intracellular                        0
                   Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine resi
YGR264C   MES1                           2256 methionyl-tRNA aminoacylation ligase synthetase (Gus1p) and
                                                               methionine-tRNA         C                                                0
                   Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNAactivity cytoplasm MES1 Arc1p, which increases the catalytic e
YFR030W   MET10                          3108 sulfate assimilation which is responsible for the
                                                               sulfite                 C           cytoplasm MET10
                   Subunit alpha of assimilatory sulfite reductase, reductase (NADPH) activity conversion of sulfite into sulfide       0
YKL001C   MET14                           609 methionine metabolism
                                                               adenylylsulfate kinase activity cytoplasm MET14
                                                                                       C
                   Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism                      0
YLR017W   MEU1                           1014 glutamate (MTAP), catalyzes the initial step in cytoplasmactivity
                                                               S-methyl-5-thioadenosine phosphorylase MEU1
                   Methylthioadenosine phosphorylase biosynthesis                      C                                                0
                                                                                                   the methionine salvage pathway; affects polyamine bios
YJL042W   MHP1                           4197 cell wall organization and biogenesisof cytoskeleton
                                                               structural constituent C            microtubule HP1
                                                                                                               M                        0
                   Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest a
YGL106W   MLC1                            450 endocytosis microfilament motor activity C           actin cap MLC1                       0
                   Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p
YNL074C   MLF3                           1359 response to drug unknown                 C           cytoplasm MLF3                       0
                   Serine-rich protein of unknown function; overproduction suppresses the growth inhibition caused by exposure to the immunosupp
YIL106W   MOB1                           1030 protein amino acid phosphorylation C
                                                               kinase regulator activity           bud activation and                   0
                   Component of the mitotic exit network; associates with and is required for theneck MOB1 Cdc15p-dependent phosphorylation
YGL178W   MPT5                           3220 cell wall organization and biogenesis
                                                               mRNA binding            C           cytoplasm MPT5                       0
                   Member of the Puf family of RNA-binding proteins; binds to mRNAs encoding chromatin modifiers and spindle pole body compon
YBR057C   MUM2                           essential for meioticunknown
                                                                DNA replication and sporulation; interacts MUM2
                   Cytoplasmic protein 1101 premeiotic DNA synthesis                   C                                                0
                                                                                                   cytoplasm with Orc2p, which is a component of the origi
YNR043W   MVD1     Mevalonate pyrophosphate decarboxylase, essential enzyme involved in cytosol
                                         1191 ergosterol biosynthesis
                                                               diphosphomevalonate decarboxylase activity
                                                                                       C            the biosynthesis of isoprenoids and sterols, including er
                                                                                                               MVD1                     0
YHR023W   MYO1                           5787 response to osmotic stress
                                                               wild-type cytokinesis C cell contractile MYO1
                                                                                       and                      ring                    0
                   Type II myosin heavy chain, required for microfilament motor activity separation; localizes to the actomyosin ring; binds to myos
YKL129C   MYO3                           3816 cell wall organization and patches; activity of actin cortical patch
                                                               microfilament motor deletion MYO3 hasMYO3
                   One of two type I myosins; localizes to actin corticalbiogenesis    C                                                0
                                                                                                                 little effect on growth, but myo3 myo5 double
YMR109W   MYO5                           3660 cell wall organization tail biogenesis2 (TH2) actin cortical patch
                                                               microfilament motor C                           MYO5
                   One of two type I myosins; contains proline-richand homologyactivity and SH3 domains; MYO5 deletion 0                 has little effect on grow
YML117W   NAB6                           3405 unknown          RNA binding             C           cytoplasm NAB6
                   Putative RNA-binding protein, based on computational analysis of large-scale protein-protein interaction data        0
YIL007C   NAS2                             to the p27 subunit of mammalian proteasome
                                                               unknown                 C           cytoplasm essential; interacts with Rpn4p
                   Protein with similarity663 ubiquitin-dependent protein catabolism modulator; not NAS2                                0
YGR232W   NAS6                            687 proteolysis      unknown                 C            human oncoprotein gankyrin, 0
                                                                                                               NAS6
                   Regulatory, non-ATPase subunit of the 26S proteasome; homolog of theproteasome regulatory particlewhich interacts with the re
YNL119W   NCS2                            urmylation and in invasive
                                                               unknown                 C           cytoplasm NCS2                       0
                   Protein with a role in1482 pseudohyphal growth and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevis
YPL174C   NIP100                         dynactin complex, which is involved in partitioning spindle pole body
                                                               protein binding         C                       NIP100                   0
                   Large subunit of the2607 establishment of mitotic spindle orientation the mitotic spindle between mother and daughter cells; pu
YLR315W   NKP2                            462 protein,
                   Non-essential kinetochoreunknownsubunit of the Ctf19 centralC
                                                               unknown                             spindle pole body
                                                                                        kinetochore complexNKP2                         0
                                                                                                                 (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-C
YLR195C   NMT1                           1368 N-terminal peptidyl-glycine covalent attachment of myristic acid
                                                               glycylpeptide N-tetradecanoyltransferase NMT1
                                                                                       C                                                0
                   N-myristoyl transferase, catalyzes the cotranslational,N-myristoylation cytosol activity to the N-terminal glycine residue of se
YLR285W   NNT1                             N-methyltransferase, has
                                                               nicotinamide in rDNAC     silencing and in lifespan determination
                                                                                                   cytoplasm
                   Putative nicotinamide786 chromatin silencing a role N-methyltransferase activity NNT1                                0
YJR072C   NPA3                           1158 aerobic ATPase; phosphorylated by the Pcl1p-Pho85p kinase complex
                   Essential, conserved, cytoplasmicrespirationprotein binding         C           cytoplasm NPA3                       0
YNL091W   NST1                           3723 response to sensitivity
                                                               unknown                 C            physically with the                 0
                   Protein of unknown function, mediates salt stress to salt stress; interacts cytoplasm NST1 splicing factor Msl1p and also display
YDR001C   NTH1                           2256 response to required for thermotolerance and cytoplasm NTH1
                   Neutral trehalase, degrades trehalose;stressalpha,alpha-trehalaseC    activity may mediate resistance to other0       cellular stresses; may b
YOR373W   NUD1     Component of the spindle microtubule nucleation constituent C cytoskeleton pole body
                                         2556 pole body outer plaque, required forof from mitosis
                                                               structural                exit      spindle     NUD1                     0
YHR073W   OSH3                           2991 steroid biosynthesis
                                                               oxysterol seven         C            cerevisiae; family                  0
                   Member of an oxysterol-binding protein family withbindingmembers in S. cytoplasm OSH3 members have overlapping, redundan
YKR003W   OSH6                           1347 steroid biosynthesis
                                                               oxysterol overlapping, redundant functions in sterol metabolism 0
                   Member of an oxysterol-binding protein family withbinding           C           unknown OSH6                          and which collectively p
YGL094C   PAN2                           the Pan2p-Pan3p poly(A)-ribonuclease complex, activity
                                                                repair                 C           cytoplasm control                    0
                   Essential subunit of 3348 postreplication poly(A)-specific ribonucleasewhich acts to PAN2 poly(A) tail length and regulate the sto
YKL025C   PAN3                           the Pan2p-Pan3p poly(A)-specific ribonuclease activity
                                                                                       C           cytoplasm control                    0
                   Essential subunit of 2040 DNA repair poly(A)-ribonuclease complex, which acts to PAN3 poly(A) tail length and regulate the sto
YJL128C   PBS2                           2007 protein amino acid the osmosensing
                                                               MAP kinase kinase activity*         cytoplasm pathway, activated under severe osmotic stre
                   MAP kinase kinase that plays a pivotal role inphosphorylation C signal-transduction PBS2                             0
YKR097W   PCK1                           1650 gluconeogenesis
                   Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glu
                                                               phosphoenolpyruvateC                cytosol     PCK1
                                                                                         carboxykinase (ATP) activity                   0
YNL264C   PDR17                          transfer protein (PITP),
                                                               phosphatidylinositol transporter cytoplasm PDR17
                                                                                       C           activity                             0
                   Phosphatidylinositol1053 response to drug downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol
YOR122C   PFY1                            590 response to 4,5-bisphosphate-binding
                                                               actin stress            C            plays a role in cytoskeleton organization, required for no
                   Profilin, actin- and phosphatidylinositolosmoticmonomer binding protein,actin cap PFY1                               0
YMR105C   PGM2                           1710 glucose 1-phosphate from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexos
                                                               phosphoglucomutaseC
                   Phosphoglucomutase, catalyzes the conversionutilization               activity cytosol      PGM2                     0
YBL051C   PIN4                           2007 G2/M transition of mitotic cell cycle C
                                                               unknown                             cytoplasm PIN4                       0
                   Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition
YOR281C   PLP2                             similarity to regulation of transcription from inhibitors; lethality of null
                                                               GTPase inhibitor activity
                                                                                       C           cytoplasm II promoter                0
                   Essential protein with861 positive phosducins, which are GTPase RNA polymerase PLP2 mutation is functionally complemente
YER132C   PMD1                           5262 kelch-like       unknown                 C           of early meiotic gene expression; required, with Mds3p,
                   Protein with an N-terminalsporulationdomain, putative negative regulator cytoplasm PMD1                              0
YOR362C   PRE10    20S proteasome alpha-type subunit
                                          867 ubiquitin-dependent protein catabolism
                                                               endopeptidase activity  C                       PRE10
                                                                                                   proteasome complex                   0
YPR103W   PRE2     20S proteasome beta-type subunit, responsible for the chymotryptic activity of the proteasome
                                          864 ubiquitin-dependent protein catabolism
                                                               endopeptidase activity  C                       PRE2
                                                                                                   proteasome complex                   0
YJL001W   PRE3     20S proteasome beta-type subunit, responsible for cleavage after acidic proteasomepeptides
                                          764 response to stress
                                                               endopeptidase activity  C           residues inPRE3 complex              0
YFR050C   PRE4     20S proteasome beta-type subunit
                                          801 ubiquitin-dependent protein catabolism
                                                               endopeptidase activity  C                       PRE4
                                                                                                   proteasome complex                   0
YMR314W   PRE5     20S proteasome alpha-type subunit
                                          705 ubiquitin-dependent protein catabolism
                                                               endopeptidase activity  C                       PRE5
                                                                                                   proteasome complex                   0
YBL041W   PRE7     20S proteasome beta-type subunit
                                          726 ubiquitin-dependent protein catabolism
                                                               endopeptidase activity  C                       PRE7
                                                                                                   proteasome complex                   0
YML092C   PRE8     20S proteasome beta-type subunit
                                          753 ubiquitin-dependent protein catabolism
                                                               endopeptidase activity  C                       PRE8
                                                                                                   proteasome complex                   0
YGR135W   PRE9                            777 ubiquitin-dependent protein catabolism
                                                               endopeptidase activity
                   20S proteasome beta-type subunit; the only nonessential 20S C                               PRE9
                                                                                        subunit proteasome complex                      0
YIL095W   PRK1                           2433 protein amino acid organization and kinase activity
                                                               protein serine/threonine            actin actinPRK1
                   Protein serine/threonine kinase; regulates thephosphorylation Cfunction of the cortical patch                         the
                                                                                                                cytoskeleton through 0 phosphorylation of
YDR300C   PRO1                           1287 proline biosynthesis
                                                               glutamate 5-kinase activity
                                                                                       C
                   Gamma-glutamyl kinase, catalyzes the first step in proline biosynthesis cytoplasm PRO1                               0
YKL181W   PRS1                           1284 histidine biosynthesisphosphate diphosphokinase activityPRS1
                                                               ribose                  C           cytoplasm                            0
                   5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five
YER099C   PRS2                             957 histidine biosynthesisphosphate diphosphokinase activityPRS2
                                                              ribose                  C          cytoplasm                           0
                   5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a fiv
YHL011C   PRS3                             963 histidine biosynthesisphosphate diphosphokinase activityPRS3
                                                              ribose                  C          cytoplasm                           0
                   5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a fiv
YBL068W   PRS4                             984 histidine biosynthesisphosphate diphosphokinase activityPRS4
                                                              ribose                  C          cytoplasm                           0
                   5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a fiv
YOL061W   PRS5                           1491 histidine biosynthesisphosphate diphosphokinase activityPRS5
                                                              ribose                  C          cytoplasm                           0
                   5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a fiv
YDL055C   PSA1                           1086 protein amino acid glycosylation
                                                              mannose0-phosphate guanylyltransferase PSA1
                                                                                      C          cytoplasm activity
                   GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and manno 0
YML017W   PSP2                           2144 unknown         unknown                 C          cytoplasm PSP2                      0
                   Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in M
YBR125C   PTC4                           1182 unknown         protein phosphatase type 2C activity
                                                                                      C          cytoplasm PTC4                      0
                   Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1
YGL014W   PUF4                           protein family, whichmRNA bindingthe presence of Pumilio homology
                                                                is defined by         C            decay                             0
                   Member of the PUF 2667 mRNA catabolism, deadenylylation-dependentcytoplasm PUF4 domains that confer RNA binding activ
YOR157C   PUP1     Endopeptidase with trypsin-like activity that cleaves after basic residues; proteasome complex20S proteasome synthesized as a
                                           786 ubiquitin-dependent protein catabolism
                                                              endopeptidase activity  C                       PUP1
                                                                                                  beta-type subunit of               0
YGR253C   PUP2     Alpha subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
                                           783 response to stress
                                                              endopeptidase activity  C                       PUP2
                                                                                                 proteasome complex                  0
YER094C   PUP3     Beta subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
                                           618 ubiquitin-dependent protein catabolism
                                                              endopeptidase activity  C                       PUP3
                                                                                                 proteasome complex                  0
YGL062W   PYC1                           3537 gluconeogenesis pyruvate carboxylase C   activity cytosol       PYC1                   to
                   Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar 0 isoform Pyc2p but di
YBR218C   PYC2                           3543 gluconeogenesis pyruvate carboxylase C   activity cytosol       PYC2                   to
                   Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar 0 isoform Pyc1p but di
YAL036C   RBG1                            family of GTP-binding proteins; interacts with translating ribosomes and with Tma46p
                   Member of the DRG1110 unknown              GTP binding             C          cytoplasm RBG1                      0
YGR173W   RBG2                           1107 unknown         GTP binding             C          cytoplasm RBG2
                   Protein with similarity to mammalian developmentally regulated GTP-binding protein                                0
YOR265W   RBL2                             321 microtubule cytoskeleton organization and biogenesis
                                                              tubulin required        C          cytoplasm free                      0
                   Protein involved in microtubule morphogenesis, binding* for protection from excessRBL2beta-tubulin; proposed to be involved the
YDL189W   RBS1                           1374 galactose metabolism
                                                              unknown                 C          cytoplasm RBS1                      0
                   Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for g
YLR248W   RCK2                           1833 the response acid phosphorylation C kinase identified RCK2
                                                              protein serine/threonine           cytoplasm                           0
                   Protein kinase involved in protein amino to oxidative and osmotic stress; activity as suppressor of S. pombe cell cycle checkpoi
YBR052C   RFS1                             633 unknown         a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent pro
                   Protein of unknown function; member of unknown                     C          cytoplasm RFS1                      0
YBR260C   RGD1                           2001 (RhoGAP) for Rho GTPase activator activity actin in control of actin cytoskeleton organization
                                                               Rho3p and Rho4p, C
                   GTPase-activating proteinosmosensory signaling pathway possibly involved cortical patch    RGD1                   0
YFL047W   RGD2                           2145 (RhoGAP) for mediated signal transduction
                                                               Cdc42p and activator
                   GTPase-activating proteinsmall GTPase Rho GTPase Rho5p Cactivity cytoplasm RGD2                                   0
YOL066C   RIB2                           1776 riboflavin biosynthesis
                                                              pseudouridylate synthase activity
                                                                                      C          cytoplasm RIB2                      0
                   DRAP deaminase, catalyzes the third step of the riboflavin biosynthesis pathway; cytoplasmic tRNA pseudouridine synthase invo
YMR139W   RIM11                          1113 protein transduction during entry into kinase cytoplasmthe formation of the Ime1p-Ume6p complex by
                                                              protein serine/threonine           promotes RIM11
                   Protein kinase required for signal amino acid phosphorylation Cmeiosis; activity                                  0
YDL001W   RMD1                           required for sporulation
                   Cytoplasmic protein 1293 unknown           unknown                 C          cytoplasm RMD1                      1
YDR255C   RMD5     Cytosolic protein required negative regulation of gluconeogenesis
                                         1266 for sporulation; also required for theC
                                                              unknown                            cytosol      RMD5                   1
                                                                                       ubiquitination of the gluconeogenetic enzyme fructose-1,6-bisphos
YFR048W   RMD8                           1989 for sporulation unknown
                   Cytosolic protein required unknown                                 C          cytoplasm RMD8                      1
YCL028W   RNQ1                           1218 unknown
                   [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate
                                                              unknown                 C          cytosol      RNQ1                   0
YER070W   RNR1                           2667 DNA replicationibonucleoside-diphosphate reductase activity
                                                              r                       C          cytoplasm RNR1
                   Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting0            step in dNTP synthesis
YHR200W   RPN10    Non-ATPase base subunitubiquitin-dependent protein catabolismthe 26S proteasome complex
                                           807 of the 19S regulatory particle activity
                                                              endopeptidase (RP) of   C                       RPN10
                                                                                                  proteasome; N-terminus plays a0     role in maintaining the
YFR052W   RPN12                            825 ubiquitin-dependent protein catabolism
                                                              endopeptidase activity  C          proteasomewith RPT1, which is an ATPase component o
                   Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethalRPN12   regulatory particle 0
YLR421C   RPN13                            471 proteolysis    endopeptidase activity
                   Subunit of the 19S regulatory particle of the 26S proteasome lid   C                       RPN13
                                                                                                 proteasome regulatory particle 0
YER021W   RPN3                           1572 ubiquitin-dependent protein catabolism
                                                              unknown                 C          proteasome regulatory particle 0
                                                                                                              RPN3
                   Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; te
YDL147W   RPN5                           1338 ubiquitin-dependent protein catabolism
                                                              unknown                 C          proteasome regulatory subunit 0
                                                                                                              RPN5
                   Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 particle and to another S. cerevis
YDL097C   RPN6                           1305 ubiquitin-dependent protein catabolism
                                                              structural molecule activity
                                                                                      C          proteasome regulatory particle 0
                                                                                                              RPN6
                   Essential, non-ATPase regulatory subunit of the 26S proteasome lid required for the assembly and activity of the 26S proteasom
YPR108W   RPN7                           1290 ubiquitin-dependent protein catabolism
                                                              structural molecule activity
                                                                                      C          proteasome regulatory particle 0
                                                                                                              RPN7
                   Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as
YOR261C   RPN8                           1017 ubiquitin-dependent protein catabolism
                                                              unknown                 C          proteasome regulatory particle 0
                                                                                                              RPN8
                   Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to an
YDR427W   RPN9                           1182 ubiquitin-dependent protein catabolism
                                                              structural molecule similarity putative proteasomal subunits
                                                                                      C                       RPN9
                   Non-ATPase regulatory subunit of the 26S proteasome, has activity to proteasome regulatory particle in0other species; null mut
YKL145W   RPT1                           of the 19S regulatory particleactivity 26S proteasome involved in regulatory particle ubiquitinated substrates;
                                                              ATPase of the
                   One of six ATPases1404 ubiquitin-dependent protein catabolism      C                       RPT1
                                                                                                 proteasome the degradation of 0
YDR394W   RPT3                           of the 19S regulatory particleactivity 26S proteasome involved in regulatory particle ubiquitinated substrates;
                                                              ATPase of the
                   One of six ATPases1287 ubiquitin-dependent protein catabolism      C                       RPT3
                                                                                                 proteasome the degradation of 0
YOR117W   RPT5                           of the 19S regulatory particleactivity 26S proteasome involved in regulatory particle ubiquitinated substrates;
                                                              ATPase of the
                   One of six ATPases1305 ubiquitin-dependent protein catabolism      C                       RPT5
                                                                                                 proteasome the degradation of 0
YCR009C   RVS161                           798 protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of0 actin cytoskeleton,
                                                                                      C
                   Amphiphysin-like lipid raft endocytosis cytoskeletal protein binding                       RVS161
                                                                                                 lipid raft, actin cortical patch     the
YDR388W   RVS167                         1449 endocytosis cytoskeletal protein bindingC          actin regulation of
                                                                                                              RVS167                 0
                   Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved incortical patch actin cytoskeleton, endocytosis, and
YDR129C   SAC6                           2040 endocytosis protein binding, bridging   C          actin the organization and maintenance of the actin cyto
                                                                                                              SAC6
                   Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) incortical patch                     0
YER129W   SAK1                            the protein amino protein kinase activityC
                                                               partially redundant function withcytoplasm SAK1 members of 1 family of kinases ha
                   Upstream kinase for3429SNF1 complex; acid phosphorylation                       Elm1p and Tos3p;                  this
YKR028W   SAP190                         complex with the Sit4p protein phosphatase phosphatase activity function; member of a family of similar pro
                                                                of mitotic cell cycle C          cytoplasm its
                   Protein that forms a 3297 G1/S transitionprotein serine/threonine and is required forSAP190                       0
YPR129W   SCD6                           1050 domain, may bind binding
                                                              RNA                      role      cytoplasm SCD6                      0
                   Protein containing an LsmRNA metabolism RNA and have aC in RNA processing; overproduction suppresses a null mutatio
YOR367W   SCP1     Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calpo
                                           603 actin filament organization
                                                              protein binding, bridging
                                                                                      C                       SCP1
                                                                                                 actin cortical patch                0
YGL083W   SCY1                           2415 unknown         unknown                 C          clathrin-coated vesicle
                   Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase SCY1                             0
YBR214W   SDS24                          1584 endocytosis unknown                     C          cytoplasm SDS24                     0
                   One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic stud
YLR166C   SEC10                          2616 of the exocyst protein binding Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which
                                                               complex (Sec3p,
                   Essential 100kDa subunit establishment of cell polarity            C          exocyst SEC10                       0
YGL233W   SEC15    Essential 113kDa subunit cytokinesis
                                         2733 of the exocyst protein binding Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which
                                                               complex (Sec3p,        C          bud tip      SEC15                  0
YNL272C   SEC2                           2280 exocytosis      guanyl-nucleotide exchange factor activity SEC2
                                                                                      C          cytosol                             0
                   Guanyl-nucleotide exchange factor for the small G-protein Sec4p, located on cytoplasmic vesicles; essential for post-Golgi vesic
YER008C   SEC3                           4011 cytokinesis     protein binding
                   Non-essential subunit of the exocyst complex (Sec3p, Sec5p, C                 bud Sec10p, Sec15p, Exo70p, Exo84p) which mediates
                                                                                      Sec6p, Sec8p,neck SEC3                         0
YPR055W   SEC8     Essential 121kDa subunit cytokinesis
                                         3198 of the exocyst protein binding Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which
                                                               complex (Sec3p,        C          bud tip      SEC8                   0
YIL074C   SER33                          dehydrogenase, catalyzes the first step inC
                                                              phosphoglycerate dehydrogenase activity SER33
                   3-phosphoglycerate 1410 serine family amino acid biosynthesis                 cytoplasm                           0
                                                                                       serine and glycine biosynthesis; isozyme of Ser3p
YDR023W   SES1                           1389 seryl-tRNA aminoacylation
                                                                aminoacyl-tRNA synthetase cytoplasm SES1
                                                                                      C                                              0
                   Cytosolic seryl-tRNA synthetase, class IIserine-tRNA ligase activity that aminoacylates tRNA(Ser), displays tRNA-dependent a
YOR165W   SEY1                           2331 membrane two predicted GTP-binding
                                                              unknown                 C          cytoplasm SEY1
                   Protein of unknown function, containsorganization and biogenesismotifs GXXXXGKS and DXXG near the2                 N-terminus, homolog o
YJL145W   SFH5                             885 phospholipid transport
                                                              phosphatidylinositol phosphatidylinositol- SFH5
                                                                                      C           activity                           0
                   Putative phosphatidylinositol transfer protein (PITP), exhibits transporter cytosol but not phosphatidylcholine-transfer activity, m
YIR001C   SGN1                             753 protein, contains an binding
                                                              poly(A)                 C          cytoplasm SGN1                      0
                   Cytoplasmic RNA-binding mRNA metabolism RNA recognition motif (RRM); may have a role in mRNA translation, as suggested
YOR007C     SGT2                          1041 unknown          unknown                C          cytoplasm SGT2                      0
                    Glutamine-rich cytoplasmic protein of unknown function; contains tetratricopeptide (TPR) repeats, which often mediate protein-pr
YKL130C     SHE2                           741 intracellular mRNA localization
                                                                mRNA and interacts with She3p; part of the mRNA localization machinery that restricts
                    RNA-binding protein that binds specific mRNAs binding              C          cytoplasm SHE2                      0
YOR035C     SHE4                          UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains
                                                                myosin binding         C                                              0
                    Protein containing a2370 actin cytoskeleton organization and biogenesiscytoplasm SHE4 to regulate myosin function; involved i
YMR140W     SIP5                          1470 cellular response to glucose starvation
                                                                unknown                C          cytoplasm the Snf1p kinase
                    Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and SIP5                             0
YLR187W     SKG3                          3081 unknown          unknown                C          cytoplasm SKG3                      0
                    Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and b
YNL243W     SLA2    Transmembrane actin-bindingwall organization in membrane cytoskeleton assembly and cell polarization;0
                                          2907 cell protein involved and biogenesis
                                                                protein binding, bridging
                                                                                       C                       SLA2
                                                                                                  actin cortical patch                 adaptor protein that lin
YGR271W     SLH1                          5904 regulation of translation
                                                                RNA helicase activityC            cytoplasm SLH1                      0
                    Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagat
YCR073W-A   SOL2                           948 tRNA-nucleus export
                                                                unknown
                    Protein with a possible role in tRNA export; shows similarity toC             cytoplasm SOL2                      0
                                                                                        6-phosphogluconolactonase non-catalytic domains but does not e
YBR194W     SOY1                           372 unknown          unknown
                    Protein proposed to be associated with the nuclear pore complex    C          cytoplasm SOY1                      0
YPL124W     SPC29   Inner plaque spindle pole body (SPB) component, links the central plaque componentbody
                                           762 microtubule nucleation constituent C cytoskeleton pole Spc42p to the inner plaque component Sp
                                                                structural             of         spindle      SPC29                  0
YKL042W     SPC42   Central plaque component of spindle pole body (SPB); involved in SPB duplication,SPC42
                                          1092 microtubule nucleation constituent C cytoskeleton pole body
                                                                structural             of         spindle                             0
                                                                                                                may facilitate attachment of the SPB to the n
YAL047C     SPC72                         1869 mitotic sister chromatid segregation C binds spindle pole body
                                                                structural constituent of         spindle      bodies                 0
                    Component of the cytoplasmic Tub4p (gamma-tubulin) complex, cytoskeleton poleSPC72 and links them to microtubules; has ro
YHR172W     SPC97   Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the0
                                          2472 microtubule nucleation constituent C cytoskeleton pole body
                                                                structural             of         spindle      SPC97                   spindle pole body (SPB
YNL126W     SPC98   Component of the microtubule-nucleatingorganization and biogenesis in nucleus pole with Spc110p at the0
                                          2541 mitotic spindle structural constituent C cytoskeleton
                                                                  Tub4p (gamma-tubulin) complex; interacts body
                                                                                       of         spindle      SPC98                   spindle pole body (SPB
YNL138W     SRV2                          1581 cytoskeleton organization and biogenesis
                                                                cytoskeletal cyclase complex; actin cortical patch
                                                                                       C                       SRV2                   0
                    CAP (cyclase-associated protein) subunit of adenylyl protein binding N-terminus binds adenylyl cyclase and facilitates activatio
YDR293C     SSD1                           maintenance of cellular integrity, interacts
                                                                RNA binding            C          cytoplasm the TOR pathway; ssd1 mutant of a clinical S
                    Protein with a role in3753 cell wall organization and biogenesis with components of SSD1                          0
YBR169C     SSE2                          shock protein 70 (HSP70) family; may be C
                    Member of the heat 2082 protein folding unknown                                 protein folding;                  0
                                                                                       involved incytoplasm SSE2localized to the cytoplasm; highly homo
YLR006C     SSK1                          2139 osmosensory a two-component activity
                                                                enzyme pathway         C          cytoplasm SSK1                      0
                    Cytoplasmic response regulator, part of signaling activator signal transducer that mediates osmosensing via a phosphorelay mec
YDR169C     STB3                          1542 a two-hybrid assay
                    Protein that binds Sin3p inunknown          unknown                C          cytoplasm STB3                      0
YCL032W     STE50                         1041 response, invasive/filamentous growth, and osmotolerance, acts
                                                                protein kinase regulator activity cytoplasm
                                                                                       C
                    Protein involved in mating signal transduction during conjugation with cellular fusionSTE50 as an adaptor0that links G protein-ass
YBL034C     STU1                          4542 microtubule nucleation constituent C cytoskeleton pole body
                                                                structural             of         spindle      STU1
                    Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin0(Tub2p); required for in
YGL169W     SUA5                          1281 aerobic respiration
                                                                unknown                C          cytoplasm SUA5                      0
                    Protein required for respiratory growth; null mutation suppresses the Cyc1p translation defect caused by the presence of an aber
YDR172W     SUP35                         2058 mRNA catabolism, deadenylylation-dependentcytosol
                                                                translation conformation activity the [PSI(+)] prion, a dominant cytoplasmically inherited
                                                                                       C
                    Translation termination factor eRF3; altered protein release factor creates decay          SUP35                  0
YBR143C     SUP45                         factor involved in translation release factor activity, acts as a recessive omnipotent suppressor
                                                                                       C          cytosol
                    Polypeptide release 1314 cytokinesis translation termination; mutant form codon specific   SUP45                  0
YPL032C     SVL3                          2478 endocytosis unknown                     C           in vacuolar function; green fluorescent protein (GFP)-fu
                    Protein of unknown function, mutant phenotype suggests a potential rolecytoplasm SVL3                             0
YLR354C     TAL1                          1008 the non-oxidative pentose activity C
                                                                transaldolase                     cytoplasm TAL1                      0
                    Transaldolase, enzyme in pentose-phosphate shunt phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehy
YNL087W     TCB2                          with potential
                    Bud-specific protein 3537 aunknown role in membrane trafficking; GFP-fusion protein migrates from the cell surface to intracellula
                                                                unknown                C          bud          TCB2                   1
YNL079C     TPM1                           600 actin filament organization actin cables
                                                                actin lateral                     contractile TPM1
                                                                                                               ring                   0
                    Major isoform of tropomyosin; binds to and stabilizes binding C and filaments, which direct polarized cell growth and the distrib
YIL138C     TPM2                           486 actin filament organization actin cables
                                                                actin lateral                     contractile TPM2
                                                                                                               ring                   0
                    Minor isoform of tropomyosin, binds to and stabilizes binding C and filaments, which direct polarized cell growth and the distrib
YBR126C     TPS1                          trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the activity carbohydrate trehalo
                    Synthase subunit of 1488 response to stress                        C          cytoplasm (UDP-forming) storage
                                                                alpha,alpha-trehalose-phosphate synthaseTPS1                          0
YMR261C     TPS3                          3165 response to stress
                                                                enzyme regulator activity
                                                                                       C          cytoplasm TPS3                      0
                    Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehal
YDL112W     TRM3                          4311 tRNA methylation ribose methylation of the guanosine nucleotide at position 18 of tRNAs
                                                                 the                   C          cytoplasm TRM3
                    2'-O-ribose methyltransferase, catalyzes tRNA (guanosine) methyltransferase activity                              0
YER090W     TRP2                          1524 tryptophan biosynthesis synthaseC
                                                                anthranilate            activity cytoplasm TRP2                       0
                    Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate s
YKL211C     TRP3                           exhibiting both indole-3-glycerol-phosphate
                                                                anthranilate            activity cytoplasm TRP3                       0
                    Bifunctional enzyme1455 tryptophan biosynthesis synthaseC synthase and anthranilate synthase activities, forms multifunctio
YDR353W     TRR1                           960 regulation of regulatory enzyme that
                                                                thioredoxin-disulfide reductase activity
                                                                                                  cytoplasm TRR1                      0
                    Cytoplasmic thioredoxin reductase, keycell redox homeostasisCdetermines the redox state of the thioredoxin system, which acts
YML100W     TSL1                          3297 6-phosphate synthase regulator activity
                    Large subunit of trehaloseresponse to stress                       C          cytoplasm TSL1
                                                                enzyme (Tps1p)/phosphatase (Tps2p) complex, which converts 0          uridine-5'-diphosphoglu
YLR212C     TUB4    Gamma-tubulin, involved in nucleating microtubules from bothC cytoskeleton and TUB4 faces of the spindle pole body
                                          1422 mitotic spindle structural constituent of cytoplasmic pole body
                                                                 organization and biogenesis in nucleus
                                                                                        the       spindle      nuclear                0
YGR080W     TWF1    Twinfilin, highly conservedbipolarmonomer-sequestering protein involved actin cortical of the cortical actin 0
                                           999 actin bud site selection
                                                                actin monomer binding  C                       TWF1
                                                                                                  in regulation patch                 cytoskeleton, compose
YDR177W     UBC1                            enzyme that mediates selective degradation of short-lived
                                                                                       C          proteasome complex
                                                                                                               UBC1                   0
                    Ubiquitin-conjugating 648 endocytosis ubiquitin conjugating enzyme activity and abnormal proteins; plays a role in vesicle bio
YBR082C     UBC4                            enzyme that mediates
                    Ubiquitin-conjugating 542 response to stressdegradation of short-lived activity
                                                                ubiquitin conjugating enzyme and abnormal complex interacts with E3-CaM in ubiquitina
                                                                                       C          proteasome proteins;
                                                                                                               UBC4                   0
YLL039C     UBI4                          1146 response to stress
                                                                protein tag            C          cytoplasm via the                   0
                    Ubiquitin, becomes conjugated to proteins, marking them for selective degradation UBI4 ubiquitin-26S proteasome system; ess
YBR058C     UBP14                         2346 protein deubiquitination
                                                                ubiquitin-specific protease activity
                                                                                       C          cytoplasm UBP14                     0
                    Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (
YMR304W     UBP15                         3693 protein deubiquitination ubiquitin precursor processing UBP15
                                                                 a role in             C
                    Ubiquitin-specific protease that may playubiquitin-specific protease activity cytoplasm                           0
YOR124C     UBP2                          3819 protein deubiquitination from ubiquitinated proteins, cleaves at the C terminus of ubiquitin fusions; capa
                                                                ubiquitin              C
                    Ubiquitin-specific protease that removes ubiquitin-specific protease activity cytoplasm UBP2                      0
YER151C     UBP3                          2739 protein deubiquitination to co-regulate activity
                                                                with Bre5p             C          cytoplasm retrograde transport between endoplasmic re
                    Ubiquitin-specific protease that interacts ubiquitin-specific proteaseanterograde andUBP3                         0
YFR010W     UBP6                          1500 protein deubiquitination
                                                                ubiquitin-specific protease activity
                                                                                        the       proteasome regulatory particle 0
                                                                                                               UBP6
                    Ubiquitin-specific protease situated in the base subcomplex ofC 26S proteasome, releases free ubiquitin from branched polyub
YGR184C     UBR1                          5853 protein monoubiquitination
                                                                ubiquitin-protein ligase activity proteasome complex
                                                                                       C                       UBR1                   0
                    Ubiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2p to ubiquitinate substrates of the N-end rule pathway; binds to the Rp
YBR006W     UGA2                          1494 response to oxidative stress utilization of gamma-aminobutyrate activity
                                                                 involved in the       C          cytoplasm UGA2
                    Succinate semialdehyde dehydrogenasesuccinate-semialdehyde dehydrogenase [NAD(P)+] (GABA) as a0                    nitrogen source; part o
YBL039C     URA7    Major CTP synthase1740 phospholipid biosynthesis
                                           isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to U
                                                                CTP synthase activity  C          cytosol      URA7                   0
YJR103W     URA8    Minor CTP synthase1695 phospholipid biosynthesis
                                           isozyme (see also URA7), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to U
                                                                CTP synthase activity  C          cytosol      URA8                   0
YNL229C     URE2    Nitrogen catabolite repression regulator that acts by inhibition of GLN3 transcriptionURE2 nitrogen source; altered form of Ure2
                                          1065 regulation of nitrogen utilization
                                                                transcription corepressor activity
                                                                                       C                        in good
                                                                                                  soluble fraction                    0
YBR105C     VID24                         1089 vesicle-mediated (vacuole
                                                                unknown                C          membraneVID24                       0
                    Peripheral membrane protein located at Vidtransport import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (F
YNL212W     VID27                         of unknown function; possibly involved in C
                    Cytoplasmic protein 2349 unknown            unknown                           cytoplasm VID27                     for
                                                                                       vacuolar protein degradation; not essential 0 proteasome-depend
YPL253C     VIK1                          complex with Kar3p microtubule motor body, possible regulator of Kar3p function in microtubule-mediated p
                                                                at the spindle pole C
                    Protein that forms a 1944 mitotic sister chromatid cohesion activity                       VIK1
                                                                                                  spindle pole body                   0
YLR410W     VIP1                          3441 actin cytoskeleton organization and biogenesiscytoplasm VIP1
                                                                unknown                C                                              0
                    Protein of unknown function probably involved in the function of the cortical actin cytoskeleton; putative ortholog of S. pombe asp
YLR360W     VPS38                         1320 Endosome 3-kinase complex that functions in unknown VPS38 Y (CPY) sorting; binds Vps30p and V
                                                                unknown
                    Part of a Vps34p phosphatidylinositol late to vacuole transport C             carboxypeptidase                    0
YPR139C     VPS66                          903 protein targeting to vacuole
                                                                 involved              C
                    Cytoplasmic protein of unknown functionunknown in vacuolar protein sorting.   cytoplasm VPS66                     1
YML097C     VPS9                          1356 protein targeting to vacuole
                                                                guanyl-nucleotide exchange factor protein transport; specifically0stimulates the intrinsic
                                                                                       C          cytosol
                    A guanine nucleotide exchange factor involved in vesicle-mediated vacuolaractivity VPS9
YLR337C     VRP1                          2454 endocytosis actin binding               C           and cortical patch
                                                                                                               VRP1                   0
                    Proline-rich actin-associated protein involved in cytoskeletal organizationactin cytokinesis; related to mammalian Wiskott-Aldrich
YDL224C   WHI4                            1950 regulation of cell size
                                                                RNA binding             C           cytoplasm the cell                 0
                    Putative RNA binding protein and partially redundant Whi3p homolog that regulatesWHI4 size requirement for passage through
YOL097C   WRS1                            1299 tryptophanyl-tRNA aminoacylation C activity cytoplasm WRS1
                                                                tryptophan-tRNA ligase
                    Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA                                          0
YPL239W   YAR1                              603 response to protein stress
                                                                unknown                 C           cytoplasm YAR1                     0
                    Cytoplasmic ankyrin-repeat containing osmotic of unknown function, proposed to link the processes of 40S ribosomal subunit biog
YER024W   YAT2                            2772 alcohol metabolism
                                                                carnitine O-acetyltransferase activity
                    Carnitine acetyltransferase; has similarity to Yat1p, which is a C              cytoplasm YAT2                     the
                                                                                         carnitine acetyltransferase associated with 0 mitochondrial outer
YBL060W   YBL060W   Hypothetical protein2064 unknown            unknown                 C           cytoplasm YBL060W                  0
YBL104C   YBL104C   Hypothetical protein3117 unknown            unknown                 C           cytoplasm YBL104C                  0
YBR056W   YBR056W   Hypothetical protein1506 unknown            unknown                 C           cytoplasm YBR056W                  0
YBR137W   YBR137W                           540 unknown          the cytoplasm; binds C Replication Protein A (RPA); YBR137W 0 not an essential gene
                    Protein of unknown function; localized to unknown                    to         cytoplasm YBR137W                  is
YCR061W   YCR061W                         1896 unknown          unknown                 C           cytoplasm YCR061W                10
                    Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YDL173W   YDL173W                           888 unknown         unknown                 C           cytoplasm YDL173W                  0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDL173W is not
YDR222W   YDR222W                         1248 unknown          unknown                 C           cytoplasm YDR222W                  0
                    Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YDR333C   YDR333C   Hypothetical protein2172 unknown            unknown                 C           cytoplasm YDR333C                  0
YDR532C   YDR532C                         1158 unknown
                    Protein of unknown function that localizes to the nuclear side of the spindle pole pole body along short spindles; deletion mutants
                                                                unknown                 C                       YDR532C
                                                                                                    spindle body and                   0
YEL020C   YEL020C                          with unknown         unknown
                    Hypothetical protein 1683 low sequence identity to Pdc1p            C           cytoplasm YEL020C                  0
YGL039W   YGL039W                         1047 unknown          oxidoreductase of the   C           cytoplasm YGL039W                  0
                    Oxidoreductase, catalyzes NADPH-dependent reduction activitybicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the
YGL185C   YGL185C                          sequence similarity to hydroxyacid activity, acting on the CH-OH group of donors, NAD or NADP as accept
                                                                                        C           cytoplasm YGL185C                  0
                    Putative protein with1140 metabolism oxidoreductase dehydrogenases; green fluorescent protein (GFP)-fusion protein localize
YGR001C   YGR001C                           902 unknown           similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion
                    Putative protein of unknown function withmethyltransferase activity C           cytoplasm YGR001C                  0
YGR122W   YGR122W                         1209 unknown          unknown                 C           cytoplasm YGR122W                  0
                    Putative protein of unknown function; deletion mutants do not properly process Rim101p and have decreased resistance to rapam
YGR210C   YGR210C                         1236 unknown          unknown                 C           cytoplasm YGR210C
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm    0
YGR272C   YGR272C                           459 processing deletion mutant
                                                                unknown                 C           cytoplasm YGR272C                  0
                    Putative protein of unknown function; of 20S pre-rRNAhas defects in pre-rRNA processing; green fluorescent protein (GFP)-fusio
YHL039W   YHL039W                         1758 unknown          unknown                 C           cytoplasm YHL039W
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm    0
YHR009C   YHR009C                         1572 unknown            an essential gene C
                    Putative protein of unknown function; notunknown                                cytoplasm YHR009C                  0
YHR192W   YHR192W                           837 unknown         unknown                 C           cytoplasm YHR192W
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm    2
YIL108W   YIL108W                         2091
                    Putative metalloprotease proteolysis                                C
                                                                metalloendopeptidase activity cytoplasm YIL108W                        0
YIL161W   YIL161W                           708 unknown         unknown                 C           cytoplasm YIL161W                  0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriche
YIR007W   YIR007W                         2295 unknown          unknown                 C           cytoplasm YIR007W                  0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a no
YJL084C   YJL084C                          of unknown functioncyclin binding with the cyclin Pcl7p; phosphorylated in vitro by the cyclin-CDK complex,
                    Cytoplasmic protein 3141 unknown             that interacts         C           cytoplasm YJL084C                  0
YLR200W   YKE2                              345 protein folding tubulin binding
                    Subunit of the heterohexameric Gim/prefoldin protein complexC                   cytoplasm YKE2                     0
                                                                                          involved in the folding of alpha-tubulin, beta-tubulin, and actin
YKL088W   YKL088W                         1716 response to salt stress
                                                                decarboxylase, may be involvedcytoplasm YKL088W
                    Predicted phosphopantothenoylcysteine purine nucleotide binding     C                                              0
                                                                                                      in coenzyme A biosynthesis; interacts with Sis2p and V
YKL151C   YKL151C                         1014 unknown          unknown                 C           cytoplasm YKL151C
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm    0
YKL171W   YKL171W                         2787 proteolysis      protein kinase activityC            cytoplasm YKL171W                  0
                    Putative protein of unknown function; predicted protein kinase; implicated in proteasome function; epitope-tagged protein localize
YKL215C   YKL215C                         3861 unknown          unknown                 C           cytoplasm YKL215C
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm    0
YLL007C   YLL007C                         1998 unknown          unknown                 C           cytoplasm YLL007C                  0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not a
YLR225C   YLR225C                         1224 unknown          unknown                 C           cytoplasm YLR225C                  0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not a
YLR287C   YLR287C                         1068 unknown          unknown                 C           cytoplasm YLR287C                  0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not a
YLR422W   YLR422W                         5799 unknown          unknown                 C           cytoplasm YLR422W                  0
                    Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p;
YML131W   YML131W                         1098 unknown            similarity            C           cytoplasm YML131W                  a
                    Putative protein of unknown function withunknown to oxidoreductases; mRNA expression is increased in 2 HOG1 and SKO1-dep
YMR073C   YMR073C                           606 unknown         unknown                  in         cytoplasm YMR073C                  0
                    Putative protein of unknown function; proposed to be involved C resistance to carboplatin and cisplatin; shares similarity to a hum
YNL152W   YNL152W                         1230 unknown
                    Protein required for cell viability         phospholipid binding C              cytoplasm YNL152W                  0
YNR047W   YNR047W                         2682 response to pheromone
                                                                protein kinase activityC            cytoplasm YNR047W                  0
                    Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; p
YOL022C   YOL022C                          of unknown function; essential gene in S288C, and non-essential with reduced growth rate in CEN.PK2
                    Cytoplasmic protein 1227 unknown            unknown                 C           cytoplasm YOL022C                  0
YOL087C   YOL087C                         3351 unknown          unknown                 C           cytoplasm YOL087C                  0
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant i
YOR118W   YOR118W   Hypothetical protein1704 unknown            unknown                 C           cytoplasm YOR118W                  0
YOR164C   YOR164C                           939 unknown         unknown
                    Protein of unknown function; interacts with Mdy2p                   C           cytoplasm YOR164C                  0
YOR220W   YOR220W                           798 unknown         unknown                 C           cytoplasm YOR220W                  0
                    Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR220W is not an esse
YPL183C   YPL183C                          of unknown functionunknown
                    Cytoplasmic protein 3042 unknown                                    C           cytoplasm YPL183C                  0
YPL184C   YPL184C                         1839 unknown
                    Putative RNA binding protein                unknown                 C           cytoplasm YPL184C                  0
YPL191C   YPL191C   Hypothetical protein1083 unknown            unknown                 C           cytoplasm YPL191C                  0
YPL199C   YPL199C   Hypothetical protein 723 unknown            unknown                 C           cytoplasm YPL199C                  0
YPR115W   YPR115W                         3252 unknown          unknown                 C           cytoplasm YPR115W
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm    0
YHR016C   YSC84                           1575 endocytosis unknown                      C           actin cortical patch
                                                                                                                similar
                    Protein involved in the organization of the actin cytoskeleton; contains SH3 domain YSC84 to Rvs167p 0
YNL241C   ZWF1                            1518 pentose-phosphate shunt, oxidative C
                                                                glucose-6-phosphate branch
                                                                                         1-dehydrogenase activity
                                                                                                     the pentose phosphate pathway; involved in adapting to
                    Glucose-6-phosphate dehydrogenase (G6PD), catalyzes the first step of cytoplasm ZWF1                               0
YGL180W   ATG1                            2694 autophagy         for autophagy and for the
                                                                                        C, kinase activity cytosol?
                                                                                                    vacuole, ATG1
                    Protein serine/threonine kinase, requiredprotein serine/threonine A cytoplasm-to-vacuole targeting (Cvt) pathway   0
YLR431C   ATG23     Peripheral membrane protein, required for autophagy and for the A
                                          1362 protein targeting to vacuole
                                                                unknown                             vacuole?, autophagosome?
                                                                                                                ATG23
                                                                                        C, cytoplasm-to-vacuole targeting (Cvt) pathway0
YDL132W   CDC53                           2448 SCF complexes (which also cycle C, N
                                                                  of mitotic cell contain Skp1p, Cdc34p, and anligase complex involved in ubiquitination;
                    Cullin, structural protein ofG1/S transitionstructural molecule activity                    CDC53
                                                                                                    SCF ubiquitin F-box protein) 0
YHR013C   ARD1                              717 protein amino acid acetylation Ard1p, Nat5p); cytoplasm acetylates many proteins, which influences
                                                                peptide (Nat1p,         C, R        N-terminally
                    Subunit of the N-terminal acetyltransferase NatA alpha-N-acetyltransferase activity ARD1                           0
YPL037C   EGD1      Subunit beta1 of the nascent polypeptide-associated complex C, R involved in protein targeting, associated with cytoplasmic rib
                                            474 nascent polypeptide association
                                                                unfolded protein binding (NAC)      cytosol     EGD1                   0
YBR101C   FES1                              873 exchange factor, cytosolic homologC, R factor activity FES1
                    Hsp70 (Ssa1p) nucleotideprotein biosynthesis                          of Sil1p, cytosol
                                                                adenyl-nucleotide exchange which is the nucleotide exchange 0          factor for BiP (Kar2p) in
YDR283C   GCN2                            4980 protein amino acid phosphorylation
                                                                protein kinase activityC, R         cytosol     (Sui2p)                0
                    Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 GCN2 in response to starvation; activated b
YFR009W     GCN20                          the Gcn2p kinase translational elongationC, R
                                                                 ATPase activity                    cytosol   GCN20                  0
                     Positive regulator of 2259 regulation of activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncha
YNL014W     HEF3     Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent bin
                                           3135 translational elongation activity
                                                                 ATPase                 C, R        cytosol   HEF3                   0
YLR244C     MAP1                           1164 proteolysis      methionyl aminopeptidase activity
                                                                                        C, R        cytosol   MAP1
                     Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent0polypeptides; function
YOR253W     NAT5                            531 protein amino acid acetylation Ard1p, Nat5p); cytoplasm acetylates many proteins, which influences
                                                                 peptide (Nat1p,        C, R        N-terminally
                     Subunit of the N-terminal acetyltransferase NatA alpha-N-acetyltransferase activity NAT5                        0
YHR170W     NMD3     Protein involved in nuclear export of large subunit assembly and maintenance
                                           1557 ribosomal the large ribosomal subunit; R as cytosol
                                                                 protein binding        C, acts               NMD3                   0
                                                                                                     a Crm1p-dependent adapter protein for export of nascen
YCR077C     PAT1     Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful0
                                           2391 chromosome segregation
                                                                 unknown                C, R        cytosol   PAT1                    chromosome transmiss
YGR085C     RPL11B   Protein component of525 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl11Ap; involved in ribosomal assembly; depletion c
                                                                                        of R        to        RPL11B                 0
YDR418W     RPL12B   Protein component of498 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl12Ap; rpl12a rpl12b double mutant exhibits slow g
                                                                                        of R        to        RPL12B                 0
YDL082W     RPL13A   Protein component of965 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl13Bp; not essential for viability; has similarity to ra
                                                                                        of R        to        RPL13A                 0
YLR029C     RPL15A   Protein component of615 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl15Bp and has similarity to 0 L15 ribosomal prote
                                                                                        of R        to        RPL15A                 rat
YMR121C     RPL15B   Protein component of615 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl15Ap and has similarity to 0 L15 ribosomal prote
                                                                                        of R        to        RPL15B                 rat
YPL220W     RPL1A    N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E
                                            654 protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPL1A                  0
YGL135W     RPL1B    N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Ap and has similarity to E
                                            654 protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPL1B                  0
YOR312C     RPL20B   Protein component of932 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl20Ap and has similarity to 0 L18a ribosomal prot
                                                                                        of R        to        RPL20B                 rat
YBR191W     RPL21A   Protein component of871 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl21Bp and has similarity to 0 L21 ribosomal prote
                                                                                        of R        to        RPL21A                 rat
YPL079W     RPL21B   Protein component of904 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl21Ap and has similarity to 0 L21 ribosomal prote
                                                                                        of R        to        RPL21B                 rat
YLR061W     RPL22A                           the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R         Rpl22Bp RPL22A
                     Protein component of755 large (60S) ribosomal subunit, has similarity tocytosol and to rat L22 ribosomal protein0
YFL034C-A   RPL22B                           the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R         Rpl22Ap RPL22B
                     Protein component of690 large (60S) ribosomal subunit, has similarity tocytosol and to rat L22 ribosomal protein0
YOL127W     RPL25                           843 protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPL25
                     Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarity to0E. coli L23 and rat L23
YLR344W     RPL26A   Protein component of831 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl26Bp and has similarity to 0 coli L24 and rat L26
                                                                                        of R        to        RPL26A                 E.
YGR034W     RPL26B   Protein component of744 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl26Ap and has similarity to 0 coli L24 and rat L26
                                                                                        of R        to        RPL26B                 E.
YFR032C-A   RPL29                            the protein biosynthesis
                                                                 structural constituent C, ribosome L29 ribosomal
                                                                                        of R         rat                             0
                     Protein component of180 large (60S) ribosomal subunit, has similarity tocytoplasm RPL29 protein; not essential for translation, b
YGL030W     RPL30                            the protein biosynthesis
                                                                 structural constituent C, ribosome L30 ribosomal
                                                                                        of R         rat                             0
                     Protein component of548 large (60S) ribosomal subunit, has similarity tocytoplasm RPL30 protein; involved in pre-rRNA process
YDL075W     RPL31A   Protein component of763 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl31Bp and has similarity to 0 L31 ribosomal prote
                                                                                        of R        to        RPL31A                 rat
YLR406C     RPL31B   Protein component of691 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl31Ap and has similarity to 0 L31 ribosomal prote
                                                                                        of R        to        RPL31B                 rat
YPL143W     RPL33A   N-terminally acetylated ribosomalbiosynthesisof the large (60S) ribosomal subunit, RPL33A
                                            849 protein proteinstructural constituent C, ribosome
                                                                  L37                   of R        cytosol                          0
                                                                                                              nearly identical to Rpl33Bp and has similarity
YOR234C     RPL33B   Ribosomal protein L37 of the large (60S)structural constituent C, ribosome to Rpl33Ap and has similarity to rat L35a; rpl33b null
                                            851 protein biosynthesis
                                                                  ribosomal subunit, nearly identical
                                                                                        of R        cytosol   RPL33B                 0
YER056C-A   RPL34A   Protein component of763 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl34Bp and has similarity to 0 L34 ribosomal prote
                                                                                        of R        to        RPL34A                 rat
YIL052C     RPL34B   Protein component of838 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl34Ap and has similarity to 0 L34 ribosomal prote
                                                                                        of R        to        RPL34B                 rat
YDL191W     RPL35A                           the protein biosynthesis
                                                                 structural constituent C, R Rpl35Bp and has similarity to rat L350ribosomal protein
                                                                                        of to
                     Protein component of854 large (60S) ribosomal subunit, identicalribosome       cytosol   RPL35A
YMR194W     RPL36A   N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Bp and has similarity to
                                            766 protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPL36A                 0
YPL249C-A   RPL36B   Protein component of541 large (60S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome Rpl36Ap and has similarity to 0 L36 ribosomal prote
                                                                                        of R        to        RPL36B                 rat
YLR185W     RPL37A                           the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R         Rpl37Bp RPL37A
                     Protein component of626 large (60S) ribosomal subunit, has similarity tocytosol and to rat L37 ribosomal protein0
YDR500C     RPL37B                           the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R         Rpl37Ap RPL37B
                     Protein component of656 large (60S) ribosomal subunit, has similarity tocytosol and to rat L37 ribosomal protein0
YJL189W     RPL39                            the protein biosynthesis
                                                                 structural constituent C, ribosome L39 RPL39
                                                                                        of R         rat                             0
                     Protein component of542 large (60S) ribosomal subunit, has similarity tocytosol ribosomal protein; required for ribosome biogen
YNL162W     RPL42A                           the protein biosynthesis
                                                                 structural constituent C, R Rpl42Bp and has similarity to rat L440ribosomal protein
                                                                                        of to
                     Protein component of833 large (60S) ribosomal subunit, identicalribosome       cytosol   RPL42A
YPR043W     RPL43A   Protein component of682 large (60S) ribosomal subunit, identicalribosome
                                             the protein biosynthesis
                                                                 structural constituent C, R Rpl43Bp and has similarity to rat L37a ribosomal protein; nu
                                                                                        of to       cytosol   RPL43A                 0
YJR094W-A   RPL43B   Protein component of554 large (60S) ribosomal subunit, identicalribosome
                                             the protein biosynthesis
                                                                 structural constituent C, R Rpl43Ap and has similarity to rat L37a ribosomal protein
                                                                                        of to       cytosol   RPL43B                 0
YML073C     RPL6A    N-terminally acetylated protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Bp and to rat L6 ribosomal
                                            946 protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPL6A                  0
YLR448W     RPL6B                            the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R         Rpl6Bp RPL6B
                     Protein component of915 large (60S) ribosomal subunit, has similarity tocytosol and to rat L6 ribosomal 0       protein; binds to 5.8S rR
YHL033C     RPL8A    Ribosomal protein L4771 protein biosynthesis
                                             of the large (60S) ribosomal subunit, nearlyR
                                                                 structural constituent C, ribosome to Rpl8Bp and has similarity to rat L7a ribosomal prot
                                                                                        of identicalcytosol   RPL8A                  0
YDL130W     RPP1B    Ribosomal protein P1622 translational elongation constituent C, ribosome
                                             beta, component of the ribosomal stalk, of R is involved in interaction of translational elongation factors w
                                                                 structural              which      cytosol   RPP1B                  0
YOL039W     RPP2A    Ribosomal protein P2321 translational elongationribosomal stalk,ribosome involved RPP2A
                                             alpha, a component of the constituent C, R
                                                                 structural             of which is cytosol                          0
                                                                                                              in the interaction between translational elonga
YDR382W     RPP2B                            beta, a componentstructural
                                                                  of the ribosomal stalk, ribosome
                                                                                        of R         involved RPP2B                  0
                     Ribosomal protein P2333 translational elongation constituent C, which iscytosol in the interaction between translational elongat
YMR230W     RPS10B                           the protein biosynthesis
                                                                 structural constituent C, ribosome Rps10Ap and has similarity to0rat ribosomal protein S
                                                                                        of R
                     Protein component of728 small (40S) ribosomal subunit; nearly identical cytosol to       RPS10B
YDR025W     RPS11A   Protein component of810 small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 riboso
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPS11A                 0
YOR369C     RPS12    Protein component of432 small (40S) ribosomal subunit; has similarity tocytosol
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome ribosomal protein S12
                                                                                        of R          rat     RPS12                  0
YMR143W     RPS16A   Protein component of976 small (40S) ribosomal subunit; identical to Rps16Bp and has similarity to E. coli S9 and rat S16 ribosom
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPS16A                 0
YDR447C     RPS17B   Ribosomal protein 51725 protein biosynthesis subunit; nearly C, ribosome
                                             (rp51) of the small structural constituent identical tocytosol
                                                                 (40s)                  of R                   and has               0
                                                                                                      Rps17Ap RPS17B similarity to rat S17 ribosomal protei
YOL121C     RPS19A   Protein component of825 small (40S) ribosomal subunit, required for assembly andRPS19A
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol                          S
                                                                                                               maturation of pre-40 0 particles; mutations in
YNL302C     RPS19B   Protein component of986 small (40S) ribosomal subunit, required for assembly andRPS19B
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol                          S
                                                                                                               maturation of pre-40 0 particles; mutations in
YGL123W     RPS2     Protein component of765 small (40S) subunit, essential for control of translational accuracy; has similarity to E. coli S5 and rat S
                                             the protein biosynthesis
                                                                 structural constituent C, ribosome
                                                                                        of R        cytosol   RPS2                   0
YKR057W     RPS21A                           the protein biosynthesis
                                                                 structural constituent C, ribosome Rps21Bp and has similarity to0rat S21 ribosomal prot
                                                                                        of R
                     Protein component of586 small (40S) ribosomal subunit; nearly identical cytosol to       RPS21A
YJL136C     RPS21B                           the protein biosynthesis
                                                                 structural constituent C, ribosome Rps21Bp and has similarity to0rat S21 ribosomal prot
                                                                                        of R
                     Protein component of724 small (40S) ribosomal subunit; nearly identical cytosol to       RPS21B
YGR118W     RPS23A   Ribosomal protein 28758 protein biosynthesis ribosomal subunit, required for translational accuracy; nearly identical to Rps23Bp
                                             (rp28) of the small structural constituent C, ribosome
                                                                 (40S)                  of R        cytosol   RPS23A                 0
YPR132W     RPS23B   Ribosomal protein 28803 protein biosynthesis ribosomal subunit, required for translational accuracy; nearly identical to Rps23Ap
                                             (rp28) of the small structural constituent C, ribosome
                                                                 (40S)                  of R        cytosol   RPS23B                 0
YLR333C     RPS25B                           the protein biosynthesis
                                                                 structural constituent C, ribosome Rps25Ap and has similarity to0rat S25 ribosomal prot
                                                                                        of R
                     Protein component of327 small (40S) ribosomal subunit; nearly identical cytosol to       RPS25B
YGL189C     RPS26A                           the protein biosynthesis
                                                                 structural constituent C, ribosome Rps26Bp and has similarity to0rat S26 ribosomal prot
                                                                                        of R
                     Protein component of360 small (40S) ribosomal subunit; nearly identical cytosol to       RPS26A
YER131W     RPS26B                           the protein biosynthesis
                                                                 structural constituent C, ribosome Rps26Ap and has similarity to0rat S26 ribosomal prot
                                                                                        of R
                     Protein component of360 small (40S) ribosomal subunit; nearly identical cytosol to       RPS26B
YHR021C   RPS27B                             the protein biosynthesis
                                                                structural constituent C, ribosome Rps27Ap and has similarity to0rat S27 ribosomal prot
                                                                                         of R
                    Protein component of799 small (40S) ribosomal subunit; nearly identical cytosol  to         RPS27B
YLR264W   RPS28B                             the protein biosynthesis
                                                                structural constituent C, ribosome Rps28Ap and has similarity to0rat S28 ribosomal prot
                                                                                         of R
                    Protein component of204 small (40S) ribosomal subunit; nearly identical cytosol  to         RPS28B
YLR388W   RPS29A                             the protein biosynthesis
                                                                structural constituent C, ribosome Rps29Bp and has similarity to0rat S29 and E. coli S14
                                                                                         of R
                    Protein component of171 small (40S) ribosomal subunit; nearly identical cytosol  to         RPS29A
YDL061C   RPS29B                             the protein biosynthesis
                                                                structural constituent C, ribosome Rps29Ap and has similarity to0rat S29 and E. coli S14
                                                                                         of R
                    Protein component of171 small (40S) ribosomal subunit; nearly identical cytosol  to         RPS29B
YOR182C   RPS30B                             the protein biosynthesis
                                                                structural constituent C, ribosome Rps30Ap and has similarity to0rat S30 ribosomal prot
                                                                                         of R
                    Protein component of603 small (40S) ribosomal subunit; nearly identical cytosol  to         RPS30B
YLR167W   RPS31                             cleaved to biosynthesis
                                                                structural constituent C, ribosome
                                                                                         of R       cytoplasm and ubiquitin; ubiquitin may facilitate assemb
                    Fusion protein that is 459 proteinyield a ribosomal protein of the small (40S) subunitRPS31                        0
YJR123W   RPS5      Protein component of678 small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal proteins; phosphorylated in v
                                             the protein biosynthesis
                                                                structural constituent C, ribosome
                                                                                         of R       cytosol     RPS5                   0
YNL096C   RPS7B     Protein component of918 small (40S) ribosomal subunit, nearly identical cytosol
                                             the protein biosynthesis
                                                                structural constituent C, ribosome Rps7Ap; interacts with Kti11p; deletion causes hyper
                                                                                         of R        to         RPS7B                  0
YPL081W   RPS9A                           1095 protein biosynthesis
                                                                structural constituent C, ribosome Rps9Bp and has
                                                                                         of R        to                                0
                    Protein component of the small (40S) ribosomal subunit; nearly identical cytoplasm RPS9A similarity to E. coli S4 and rat S9 rib
YBR189W   RPS9B     Protein component of the small (40S) ribosomal subunit; nearly identical cytosol
                                          1001 protein biosynthesis
                                                                structural constituent C, ribosome Rps9ApRPS9B similarity to E. coli S4 and rat S9 rib
                                                                                         of R        to          and has               0
YNL007C   SIS1                            1059 protein interacts with the HSP70 protein  C,         cytosol     SIS1                   0
                    Type II HSP40 co-chaperone thatfolding unfolded protein binding R Ssa1p; not functionally redundant with Ydj1p due to due to su
YIR012W   SQT1                            1296 in a late step of 60S ribosomal subunit R
                                                                unknown                  C, assembly or
                                                                                                    cytoplasm SQT1                     0
                    Essential protein involved ribosomal large subunit assembly and maintenance modification; contains multiple WD repeats; intera
YLR150W   STM1                              quadruplex and purine motif
                                                                DNA binding              C, R       cytoplasm STM1                     0
                    Protein that binds G4822 telomere maintenance* triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts
YPR041W   TIF5      Translation initiation1218 regulation of translational initiation factor activity
                                           factor eIF-5; N-terminal domain functions C, R GTPase-activating protein to mediate 0
                                                                translation              as a       cytosol     TIF5                   hydrolysis of ribosome-
YOR359W   VTS1      Post-transcriptional gene regulator, RNA-binding protein containing a SAM domain;VTS1 genetic interactions with Vti1p, which
                                          1572 protein targeting to vacuole
                                                                RNA binding              C, R       cytosol      shows                 0
YGR054W   YGR054W                         factor (eIF) 2A; associates
                                                                translation initiation factor activity
                                                                                         C, R         subunits YGR054W                 0
                    Eukaryotic initiation 1929 translational initiation specifically with both 40Scytosol and 80 S ribosomes, and interacts genetically
YBR110W   ALG1      Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutati
                                          1350 protein amino acid N-linked glycosylation
                                                                beta0,4-mannosyltransferase activity
                                                                                         E          ER          ALG1                   1
YNL048W   ALG11                           1647 protein amino acid glycosylation
                                                                alpha0,2-mannosyltransferase ER
                                                                                         E          1,2-Man the Man5GlcNAc2-PP-dolichol intermediate
                    Alpha-1,2-mannosyltransferase, catalyzes addition of the terminal alphaactivity toALG11                            1
YNR030W   ALG12     Alpha-1,6-mannosyltransferase localizedalpha0,6-mannosyltransferase addition of the alpha-1,6 mannose to dolichol-linked Man
                                          1656 protein amino acid glycosylation
                                                                  to the ER; responsible for the activity
                                                                                         E          ER          ALG12                  9
YGL065C   ALG2                            1512 oligosaccharide-lipid intermediate assembly activity
                                                                glycolipid mannosyltransferase ER pathway; alg2 mutants exhibit temperature-sensitive
                    Presumed early mannosyltransferase involved in the N-linked E        glycosylation          ALG2                   4
YBL082C   ALG3      Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in activity
                                          1377 protein amino acid glycosylation
                                                                alpha0,3-mannosyltransferase the synthesis of dolichol-linked oligosaccharide donor for
                                                                                         E          ER          ALG3                   9
YPL227C   ALG5      UDP-glucose:dolichyl-phosphate glucosyltransferase, glycosylation
                                          1005 protein amino acid N-linked involvedE asparagine-linkedALG5
                                                                dolichyl-phosphate beta-glucosyltransferase activity
                                                                                          in        ER           glycosylation in the endoplasmic reticulum
                                                                                                                                       1
YOR002W   ALG6                            1635 protein amino transferase activity, from dolichyl pyrophosphate to asparagine 10
                                                                 of oligosaccharides E
                    Glucosyltransferase, involved in transfer acid glycosylation transferring ER                ALG6
                                                                                                     hexosyl groups                   residues of proteins duri
YOR067C   ALG8      Glucosyl transferase, involved in N-linked glycosylation; adds glucose to ER dolichol-linked oligosaccharide precursor prior to tra
                                          1734 protein amino acid N-linked glycosylation
                                                                dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha0,3-glucosyltransferase activity
                                                                                         E           the        ALG8                  12
YNL219C   ALG9                            1668 protein amino acid glycosylation
                                                                mannosyltransferase E    activity ER            ALG9                   7
                    Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosa
YCR048W   ARE1                            1833 sterol isozyme of O-acyltransferase activity ER
                                                                sterol                   E
                    Acyl-CoA:sterol acyltransferase,metabolism Are2p; endoplasmic reticulum enzyme ARE1                                9
                                                                                                                that contributes the major sterol esterification
YNR019W   ARE2                            1929 sterol isozyme of O-acyltransferase activity ER
                                                                sterol                   E
                    Acyl-CoA:sterol acyltransferase,metabolism Are1p; endoplasmic reticulum enzyme ARE2                                9
                                                                                                                that contributes the major sterol esterification
YHR101C   BIG1      Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
                                          1095 cell wall biosynthesis
                                                                unknown                  E          ER          BIG1                   1
YLR078C   BOS1                              824 SNAP receptor), localized to the endoplasmicER, golgi membrane and necessary for vesicular transp
                                                                v-SNARE activity
                    v-SNARE (vesicle specificER to Golgi transport                       E            reticulum BOS1                   1
YFL025C   BST1      GPI inositol deacylase of the ERGolginegatively regulates COPII vesicle ER
                                          3090 ER to that transport
                                                                carboxylic ester hydrolase activity
                                                                                         E                      prevents production of vesicles with defective
                                                                                                     formation, BST1                   8
YDR182W   CDC1                            protein of repair     unknown                  E          membraneCDC1                       3
                    Putative membrane 1476 DNAunknown function involved in Mn2+ homeostasis; mutants display actin and general growth defect
YNL111C   CYB5      Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; required for sterol C5-6 and fatty acid desaturation
                                            363 sterol biosynthesis
                                                                electron transporter activity
                                                                                         E          ER          CYB5                   1
YDR411C   DFM1                            1026 unknown
                    ER localized derlin-like family member involved in ER stress and homeostasis; not involved in ERAD or substrate retrotranslocat
                                                                unknown                  E          ER          DFM1                   4
YGR227W   DIE2      Dolichyl-phosphoglucose-dependent glucosyltransferase of the ER, functions in theDIE2
                                          1578 protein amino acid N-linked glycosylation
                                                                dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity
                                                                                         E          ER           dolichol pathway that synthesizes the dolicho
                                                                                                                                      11
YDR294C   DPL1                            phosphate lyase, metabolism
                                                                sphinganine0-phosphate of sphingolipid DPL1
                                                                                         E          ER                                 0
                    Dihydrosphingosine 1770 sphingolipid regulates intracellular levelsaldolase activitylong-chain base phosphates (LCBPs), degrade
YOR092W   ECM3      Non-essential protein of unknown function
                                          1842 cell wall organization and biogenesis
                                                                ATPase activity          E          ER          ECM3                   8
YIL005W   EPS1                            2106 protein retention protein involved in endoplasmic
                                                                protein                  E          ER          EPS1
                    Pdi1p (protein disulfide isomerase)-related in ERdisulfide isomerase activity reticulum retention of resident1ER proteins
YML126C   ERG13     3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes thesynthase activity
                                          1476 ergosterol biosynthesis
                                                                hydroxymethylglutaryl-CoA formation of HMG-CoA from acetyl-CoA and acetoacetyl-Co
                                                                                         E          Mito        ERG13                  0
YNL280C   ERG24                           1317 ergosterol biosynthesis
                                                                 biosynthesis; reductase activityER
                                                                                         E                      ERG24
                    C-14 sterol reductase, acts in ergosterol delta14-sterol mutants accumulate the abnormal sterol ignosterol8(ergosta-8,14 dienol), a
YER044C   ERG28     Endoplasmic reticulum membrane protein, may facilitate bridging
                                            447 ergosterol biosynthesis
                                                                protein binding, protein-protein interactions between the Erg26p 2
                                                                                         E          ER          ERG28                  dehydrogenase and the
YMR015C   ERG5                            1617 cytochrome P450 enzyme that catalyzes the ER
                                                                C-22 sterol              E
                    C-22 sterol desaturase, a ergosterol biosynthesis desaturase activityformation ofERG5                              0
                                                                                                                 the C-22(23) double bond in the sterol side c
YFR041C   ERJ5                              888 unknown
                    Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic 2
                                                                unknown                  E          ER          ERJ5                   reticulum; loss of the no
YML130C   ERO1                            1692 oxidative protein folding in the endoplasmic reticulum
                    Thiol oxidase required for protein folding electron carrier activity E          ER          ERO1                   0
YPR037C   ERV2                              591 protein thiol-disulfide exchange
                                                                 to the endoplasmic E               microsome   ERV2                   1
                    Flavin-linked sulfhydryl oxidase localizedthiol oxidase activity reticulum lumen, involved in disulfide bond formation within the ER
YMR052W   FAR3      Protein involved in G1 cellcell cycle arrestresponse to pheromone, in a pathway different from the Far1p-dependent pathway; inte
                                            615 cycle arrest inunknown
                                                                   in response to pheromone
                                                                                         E          ER          FAR3                   0
YCR034W   FEN1      Fatty acid elongase,1044 vesicle-mediated transport
                                           involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regul
                                                                fatty acid elongase activity
                                                                                         E          ER          FEN1                   7
YAL028W   FRT2      Tail-anchored endoplasmic reticulum membrane protein, interacts with homolog Frt1p but is not a substrate of calcineurin (unlike
                                          1587 response to stress
                                                                unknown                  E          ER          FRT2                   1
YLR088W   GAA1                            1845 attachment of GPI anchor to protein E
                                                                GPI-anchor transamidase activity    GPI-anchor transamidase complex
                                                                                                                GAA1                   6
                    Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches
YDR302W   GPI11     ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of ph
                                            660 GPI anchor biosynthesis
                                                                phosphoethanolamine N-methyltransferase activity
                                                                                         E          ER          GPI11                  4
YLL031C   GPI13                           3054 GPI anchor biosynthesis activity, transferring ER
                                                                transferase              E           phosphorus-containing groups residue of the glycosylph
                                                                                                                GPI13
                    ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose13
YHR188C   GPI16                           1833 attachment of GPI anchor to protein E
                                                                GPI-anchor transamidase transamidase complex that adds GPIs to newly synthesized p
                    Transmembrane protein subunit of the glycosylphosphatidylinositol activity                  GPI16
                                                                                                    GPI-anchor transamidase complex    1
YDR434W   GPI17                           1605 attachment of GPI anchor to protein E
                                                                GPI-anchor transamidase transamidase complex that adds GPIs to newly synthesized p
                    Transmembrane protein subunit of the glycosylphosphatidylinositol activity                  GPI17
                                                                                                    GPI-anchor transamidase complex    2
YDR331W   GPI8                            1236 attachment the glycosylphosphatidylinositol transamidase transamidase adds glycosylphosphatidylinos
                                                                GPI-anchor transamidase activity
                    ER membrane glycoprotein subunit ofof GPI anchor to protein E                               GPI8
                                                                                                    GPI-anchor complex that complex    1
YMR161W   HLJ1                              675 protein folding chaperone regulator activity
                                                                                         E          ER          HLJ1                   1
                    Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Hdj1p promotes ER-associated protein degradation
YLR205C   HMX1      ER localized, heme-binding peroxidase involved in the degradation of heme; does not exhibit heme oxygenase activity despite si
                                            954 iron ion homeostasis binding
                                                                heme                     E          ER          HMX1                   1
YLR207W   HRD3                            2502 ER-associatedubiquitin-protein ligase ER-associated protein degradation (ERAD), forms HRD comple
                                                                 protein a central role in
                    Resident protein of the ER membrane that plays catabolism E activity ER                     HRD3                   1
YJR118C   ILM1                              612 mitochondrial genome maintenance DNA maintenance; required for slowed DNA synthesis-induced fila
                                                                unknown
                    Protein of unknown function; may be involved in mitochondrialE                  ER          ILM1                   3
YOL065C   INP54                            4,5-bisphosphate inositol-polyphosphate 5-phosphatase activity
                                                                                         E          ER          INP54                  0
                    Phosphatidylinositol1155 exocytosis 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-te
YJL073W   JEM1                            1938 protein folding membrane fusion during    E          ER           to the                0
                    DnaJ-like chaperone required for nuclearunfolded protein binding mating, localizesJEM1 ER membrane; exhibits genetic interac
YIL027C     KRE27                            426 unknown         shows K1
                     Protein of unknown function; null mutant unknown killer toxin E     resistance ER          KRE27                  2
YOR336W     KRE5     Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
                                           4098 beta0,6 glucanUDP-glucose:glycoprotein glucosyltransferase activity
                                                                   biosynthesis          E          ER          KRE5                   0
YKL008C     LAC1     Ceramide synthase 1257 ceramide biosynthesis
                                            component, involvedsphingosine N-acyltransferase C26(acyl)-coenzyme A and dihydrosphingosine or phytos
                                                                   in synthesis of ceramide from activity
                                                                                         E          ER          LAC1                   7
YHL003C     LAG1     Ceramide synthase 1236 replicative cell sphingosine N-acyltransferase C26(acyl)-coenzyme A and dihydrosphingosine or phytos
                                            component, involvedaging
                                                                   in synthesis of ceramide from activity
                                                                                         E          ER          LAG1                   7
YDR062W     LCB2                           1686 sphingolipid biosynthesis
                                                                 serine C-palmitoyltransferase activity first committed step in sphingolipid synthesis, wh
                                                                                         E           for the
                     Component of serine palmitoyltransferase, responsible along with Lcb1p membraneLCB2                               0
YKL073W     LHS1     Molecular chaperone of the endoplasmicunfolded protein binding in polypeptide translocation and folding; member of the Hsp7
                                           2646 response to unfolded protein
                                                                  reticulum lumen, involved
                                                                                         E          ER          LHS1                   1
YNR008W     LRO1                            catalyzes diacylglycerol esterification; one of several acyltransferases that contribute1to triglyceride synthesi
                                                                 phospholipid:diacylglycerol acyltransferaseLRO1
                     Acyltransferase that1986 triacylglycerol biosynthesis               E          ER            activity
YIR033W     MGA2                           3342 positive
                     ER membrane protein involved in regulation of OLE1 transcription, actspolymerase MGA2
                                                                 transcriptional activator activity with homologpromoter inactive ER form dimerizes and on
                                                                         transcription from RNA ER
                                                                                         E                      II Spt23p;             1
YHR204W     MNL1                           2391 ER-associatedcarbohydrate binding E
                                                                  protein catabolism                ER          MNL1                   0
                     Alpha mannosidase-like protein of the endoplasmic reticulum required for degradation of glycoproteins but not for processing of N
YJR131W     MNS1     Alpha-1,2-mannosidase involved in ER quality control;glycosylation 1,2-alpha-mannosidase activity
                                           1650 protein amino acid N-linked catalyzes the removal of one mannose residue from Man9GlcNAc to prod
                                                                 mannosyl-oligosaccharideE          ER          MNS1                   1
YOL088C     MPD2                             834 protein folding protein family, exhibits chaperone
                                                                                         E          ER
                     Member of the protein disulfide isomerase (PDI) disulfide isomerase activity activity;MPD2                        1
                                                                                                                 overexpression suppresses the lethality of a
YBR170C     NPL4                           1743 ER-associatedunknown
                                                                  protein catabolism E               with       NPL4                   0
                     Endoplasmic reticulum and nuclear membrane protein, forms a complex ER Cdc48p and Ufd1p that recognizes ubiquitinated pr
YHR133C     NSG1                             876 unknown         unknown                 E          unknown NSG1                       5
                     Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyz
YNL156C     NSG2                             900 unknown
                     Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyz
                                                                 unknown                 E          ER          NSG2                   2
YML059C     NTE1                             deacylates exogenous lysophospholipids,Ehomolog of human neuropathy target esterase (NTE); mammalian
                                                                 hydrolase activity
                     Serine esterase that5040 phosphatidylcholine metabolism                        ER          NTE1                   3
YGR038W     ORM1     Evolutionarily conserved protein with similarity to Orm2p, required for resistance to ORM1 that induce the unfolded protein respo
                                             669 response to unfolded protein
                                                                 unknown                 E          ER          agents                 3
YLR350W     ORM2     Evolutionarily conserved protein with similarity to Orm1p, required for resistance to ORM2 that induce the unfolded protein respo
                                             651 response to unfolded protein
                                                                 unknown                 E          ER          agents                 4
YJL002C     OST1     Alpha subunit of the1431 protein amino acid N-linked glycosylation
                                            oligosaccharyltransferase complex of the E lumen,ER
                                                                 dolichyl-diphosphooligosaccharide-protein OST1 asparagine-linked glycosylation of new
                                                                                         ER           which catalyzes
                                                                                                                glycotransferase activity
                                                                                                                                       1
YOR103C     OST2     Epsilon subunit of the393 protein amino acid N-linked glycosylation lumen, which catalyzes asparagine-linked glycosylation of ne
                                               oligosaccharyltransferase complex of the ER
                                                                 dolichyl-diphosphooligosaccharide-protein OST2
                                                                                         E          ER                                 3
                                                                                                                glycotransferase activity
YOR085W     OST3                           1053 protein complex assembly
                                                                 dolichyl-diphosphooligosaccharide-protein glycotransferase activity
                                                                                         E          membraneOST3                       4
                     Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of n
YGL226C-A   OST5     Zeta subunit of the oligosaccharyltransferase N-linked glycosylation
                                             410 protein amino acid complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly
                                                                 dolichyl-diphosphooligosaccharide-protein OST5
                                                                                         E          ER                                 2
                                                                                                                glycotransferase activity
YML019W     OST6     Subunit of the oligosaccharyltransferase dolichyl-diphosphooligosaccharide-protein OST6
                                             999 protein complex assembly the ER lumen, which catalyzesglycotransferase activity
                                                                 complex of              E          ER                                 4
                                                                                                                 asparagine-linked glycosylation of newly syn
YCL052C     PBN1                           1251 GPI anchor biosynthesis
                                                                 mannosyltransferase E   activity ER            PBN1
                     Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic 1        post-translational proce
YMR123W     PKR1                             369 unknown
                     V-ATPase assembly factor, functions with other V-ATPase assembly factors in the PKR1 efficiently assemble the V-ATPase mem
                                                                 unknown                 E          ER          ER to                  2
YOR321W     PMT3     Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine res
                                           2262 protein amino acid N-linked glycosylation
                                                                 dolichyl-phosphate-mannose-protein mannosyltransferase activity
                                                                                         E          ER          PMT3                 10
YDL093W     PMT5     Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine res
                                           2232 protein amino acid N-linked glycosylation
                                                                 dolichyl-phosphate-mannose-protein mannosyltransferase activity
                                                                                         E          ER          PMT5                 11
YGR199W     PMT6     Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of s
                                           2280 protein amino acid N-linked glycosylation
                                                                 dolichyl-phosphate-mannose-protein mannosyltransferase activity
                                                                                         E          ER          PMT6                   8
YMR022W     QRI8                              enzyme, involved in the or disassemblyE activity ER
                                                                 ubiquitin-protein ligase                       QRI8                   0
                     Ubiquitin conjugating 498 chromatin assemblyER-associated protein degradation pathway; requires Cue1p for recruitment to the
YER083C     RMD7     Subunit of the GET complex; required for meiotic nuclear division and forER retrieval of HDEL proteins from the Golgi to the ER
                                             858 cell wall organization and biogenesis
                                                                 unknown                 E            the       RMD7                   3
YMR214W     SCJ1                           1134 protein folding chaperone binding E
                     One of several homologs of bacterial chaperone DnaJ, located in the ERER                   SCJ1
                                                                                                     lumen where it cooperates with 1  Kar2p to mediate matur
YGL126W     SCS3     Protein required for inositol prototrophy,metabolism be involved in the synthesis of inositol phospholipids from inositol but not in t
                                           1143 phospholipid appears to
                                                                 unknown                 E          ER          SCS3                   5
YMR272C     SCS7     Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has bo
                                           1155 fatty acid metabolism
                                                                 oxidoreductase activity E          ER          SCS7                   3
YBL011W     SCT1     Glycerol 3-phosphate/dihydroxyacetonebiosynthesisdual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis p
                                           2280 phospholipid phosphate
                                                                 glycerol-3-phosphate E             ER          SCT1
                                                                                          O-acyltransferase activity                   4
YIR022W     SEC11    18kDa catalytic subunit of signal peptide processing
                                             504 the Signal Peptidasepeptidase (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal seq
                                                                 signal Complex activity E          ER          SEC11                  0
YNR026C     SEC12                           exchange factor (GEF); glycosylated integral membrane protein of the endoplasmic reticulum, important for t
                                                                 guanyl-nucleotide exchange factor activity SEC12
                     Guanine nucleotide 1416 ER to Golgi transport                       E          ER                                 1
YDR498C     SEC20                          1152 vesicle involved in retrograde transport from the Golgi toSEC20 required for N- and O-glycosylation i
                     Membrane glycoprotein v-SNAREfusion v-SNARE activity                E          ER           the ER;               0
YLR378C     SEC61    Essential subunit of 1443 protein targeting to ERtransporter activityforms ER
                                            Sec61 complex (Sec61p, Sbh1p, and Sss1p);
                                                                 protein                 E                      for SRP-dependent protein import and retrog
                                                                                                     a channel SEC61                   5
YPL094C     SEC62                            825 posttranslational protein-membrane E
                                                                 protein binding         targeting ER           SEC62                  2
                     Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a
YBR171W     SEC66    Non-essential subunit621Sec63 complex protein transporter Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p form
                                               of filamentous growth
                                                                  (Sec63p, Sec62p, activity
                                                                                         E          ER          SEC66                  1
YLR292C     SEC72                              of posttranslational protein-membrane E
                                                                  (Sec63p, Sec62p, activity
                                                                                         targeting ER           SEC72                  0
                     Non-essential subunit582Sec63 complex protein transporter Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p form
YCR067C     SED4     Integral endoplasmic reticulum membrane protein, functions as a positive regulator SED4
                                           3198 ER to Golgi transport
                                                                 unknown                 E          ER                                 0
                                                                                                                of Sar1p probably through inhibition of GTPa
YOL031C     SIL1     Nucleotide exchange factor for the endoplasmic reticulum protein-membrane targeting, translocation
                                           1266 SRP-dependent cotranslational (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocatio
                                                                 unknown                 E          ER          SIL1                   0
YOR307C     SLY41                          1362 ER to Golgi transport
                     Protein involved in ER-to-Golgi transport unknown                   E          ER          SLY41                  8
YJL192C     SOP4     ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the
                                             705 ER to Golgi transport
                                                                 unknown                 E          ER          SOP4                   0
YJR010C-A   SPC1     Subunit of the signal peptidase complex processing cleavesE signalsignal peptidaseproteins targeted to the endoplasmic ret
                                             285 signal peptide (SPC), which
                                                                 unknown                  the                   from
                                                                                                     sequence SPC1 complex             2
YML055W     SPC2     Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences
                                             537 signal peptide processing
                                                                 protein binding         E                      SPC2
                                                                                                    signal peptidase complex           2
YLR066W     SPC3     Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences
                                             555 signal peptide processing
                                                                 signal peptidase activity
                                                                                         E                      SPC3
                                                                                                    signal peptidase complex           1
YDR292C     SRP101   Signal recognition particle protein receptor -to ER subunit; contain GTPase domains; involved in SRP-dependent protein targetin
                                           1866 (SRP) targeting alpha
                                                                 GTP binding             E          ER          SRP101                 0
YKL154W     SRP102   Signal recognition particle protein receptor beta subunit; involved in SRP-dependentSRP102targeting; anchors Srp101p to the ER
                                             735 (SRP) targeting to ER activity
                                                                 GTPase                  E          ER           protein               1
YBR283C     SSH1                            translocon complex; Sec61p homolog activity
                                                                  protein-membrane targeting ER
                                                                                         E                      SSH1
                     Subunit of the Ssh1 1473 cotranslational protein transporterinvolved in co-translational pathway of protein 9     translocation; not essen
YDR086C     SSS1                             243 SRP-dependent cotranslational protein-membrane targeting, translocation
                                                                 protein transporter activity
                                                                                         E          ER          SSS1
                     Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of1secretory proteins thro
YGL022W     STT3     Subunit of the oligosaccharyltransferase acid N-linked glycosylation which catalyzesSTT3
                                           2157 protein amino dolichyl-diphosphooligosaccharide-protein glycotransferase activity
                                                                 complex of the ER lumen,E          ER                               13
                                                                                                                 asparagine-linked glycosylation of newly syn
YDR297W     SUR2                           1050 sphingolipid biosynthesis hydroxylase activity phytosphingosine in sphingolipid biosyntheis
                                                                 sphingosine             E
                     Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine toER                   SUR2                   4
YLR372W     SUR4     Elongase, involved in fattysphingolipid biosynthesis
                                           1038 acid and sphingolipid biosynthesis; E
                                                                 fatty acid elongase activity
                                                                                          synthesizes very long chain 20-26-carbon fatty acids from C18-Co
                                                                                                    ER          SUR4                   6
YDR320C     SWA2     Auxilin-like protein involved in organization and biogenesis
                                           2007 ER vesicular transport; clathrin-binding proteinER
                                                                 protein binding         E                      SWA2                   0
                                                                                                      required for uncoating of clathrin-coated vesicles
YMR149W     SWP1     Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in th
                                             861 protein amino acid N-linked glycosylation
                                                                 dolichyl-diphosphooligosaccharide-protein SWP1
                                                                                         E          ER                                 2
                                                                                                                glycotransferase activity
YGL145W     TIP20                          2106 retrograde transport, Golgi to ER E
                                                                 unknown                            ER          TIP20                  0
                     Peripheral membrane protein required for fusion of COPI vesicles with the ER, prohibits back-fusion of COPII vesicles with the E
YER100W     UBC6     Ubiquitin-conjugating 753 protein monoubiquitination
                                              enzyme involved inubiquitin conjugating enzyme activity located at the cytosolic side of the ER membrane;
                                                                   ER-associated protein degradation;
                                                                                         E          ER          UBC6                   1
YGR048W     UFD1     Protein that interacts with ubiquitin-dependent involvedcatabolism
                                           1086 Cdc48p and Npl4p, protein in recognition of polyubiquitinated proteins and their presentation to the 26
                                                                 protein binding         E          ER          UFD1                   0
YOR075W   UFE1                           1041 vesicle fusion t-SNARE activity            E
                    t-SNARE required for ER membrane fusion and vesicular traffic, integral ER                  UFE1                   0
                                                                                                     membrane protein that constitutes with Sec20p and Use
YML029W   USA1                           2517 nuclear proteinsplicing,
                                                                 degradation (ERAD); component of the Hrd1p complex; interacts with the U1 snRNP-s
                    Protein involved in ER-associated mRNAunknown via spliceosome        E          unknown USA1                       2
YGL098W   USE1      Essential SNARE protein localized to theSNAP receptor activity
                                          738 ER to Golgi transport
                                                                 ER, involved in retrograde traffic from the USE1 to the ER; forms a complex with the SN
                                                                                         E          ER           Golgi                 1
YHR060W   VMA22     Integral membrane protein that is complex assembly H+-ATPase (V-ATPase) function, although not an0
                                          546 protein required for vacuolar binding
                                                                unfolded protein         E          ER          VMA22                  actual component of th
YKL119C   VPH2      Integral membrane protein required for vacuolar H+-ATPase (V-ATPase)ER
                                          648 protein complex assembly
                                                                unknown                  E                      VPH2                   component of the V-AT
                                                                                                      function, although not an actual 2
YCL045C   YCL045C                        2283 unknown
                    Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; interacts 2
                                                                unknown                  E          ER          YCL045C                with Gal80p; YCL045C
YPL087W   YDC1                            954 response to heat  ceramidase activity E               ER          YDC1                   7
                    Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid an
YDL121C   YDL121C   Hypothetical protein 450 unknown            unknown                  E          ER          YDL121C                1
YDR056C   YDR056C                         618 unknown
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic retiuculum ;YD
                                                                unknown                  E          ER          YDR056C                0
YDR221W   YDR221W                        subunit, forms
                    Glucosidase II beta 2109 unknowna complex with alpha subunit Rot2p, involved in YDR221W two glucose residues from N-linke
                                                                unknown                  E          ER           removal of            0
YDR307W   YDR307W   Hypothetical protein1989 unknown            unknown                  E          ER          YDR307W                5
YEL001C   YEL001C                         678 unknown
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null
                                                                unknown                  E          ER          YEL001C                1
YDL072C   YET3                            612 unknown
                    Protein of unknown function; YET3 null mutant decreases the E
                                                                unknown                             ER          YET3                   3
                                                                                         level of secreted invertase; homolog of human BAP31 protein
YGL231C   YGL231C                         573 unknown
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
                                                                unknown                  E          ER          YGL231C                2
YGR263C   YGR263C                        1275 unknown
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
                                                                unknown                  E          ER          YGR263C                1
YHR045W   YHR045W                        1683 unknown
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
                                                                unknown                  E          ER          YHR045W                1
YIL039W   YIL039W                        1422 unknown
                    Putative protein of unknown function; GFP-fusion protein localizes to the ER
                                                                unknown                  E                      YIL039W
                                                                                                     endoplasmic reticulum; deletion 1 confers sensitivity to 4-
YJR088C   YJR088C                         879 unknown
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
                                                                unknown                  E          ER          YJR088C                0
YLR050C   YLR050C                         486 unknown
                    Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR
                                                                unknown                  E          ER          YLR050C                3
YLR301W   YLR301W                         735 cotranslational unknown
                                                                  with Sec72p            E
                    Protein of unknown function that interactsprotein-membrane targeting ER                     YLR301W                0
YML125C   YML125C   Essential protein required unknown
                                          939 for maturation unknown and Pho8p,E
                                                                of Gas1p                            ER          YML125C                2
                                                                                          protein trafficking; GFP-fusion protein localizes to the endoplasmi
YDR057W   YOS9      ER quality-control lectin; integralGolgi transport HRD ligase; activity as a receptor for misfolded N-glycosylated proteins, participa
                                         1629 ER to subunit protein transporter serves
                                                                of the                   E          ER          YOS9                   0
YPL207W   YPL207W   Hypothetical protein2433 unknown            unknown                  E          ER          YPL207W                1
YPR003C   YPR003C   Hypothetical protein2265 unknown            unknown                  E          ER          YPR003C              10
YPR063C   YPR063C                         509 unknown
                    ER-localized protein of unknown functionunknown                      E          ER          YPR063C                0
YPR114W   YPR114W   Hypothetical protein 948 unknown            unknown                  E          ER          YPR114W                5
YGR177C   ATF2                           1608 may play a role inalcohol O-acetyltransferase activity
                                                                                         E, C        volatile esters during fermentation, which is important in
                    Alcohol acetyltransferase, steroid metabolismsteroid detoxification; formscytoplasm ATF2                           0
YML101C   CUE4                            354 unknown           domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
                    Protein of unknown function; has a CUE unknown                       E, C       cytoplasm CUE4                     1
YKR067W   GPT2                           2232 phospholipid biosynthesis
                                                                glycerol-3-phosphate E, C O-acyltransferase activity
                                                                                                    cytoplasm GPT2                     4
                    Glycerol-3-phosphate acyltransferase located in both lipid particles and the ER; involved in the stepwise acylation of glycerol-3-p
YBR172C   SMY2                           2223 cytoskeleton organization and biogenesis
                                                                unknown                  E, C       cytoplasm SMY2
                    Protein of unknown function that interacts with Myo2p; has similarity to S. pombe Mpd2                             0
YDL122W   UBP1                           2430 protein deubiquitination from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irresp
                                                                ubiquitin                E, C
                    Ubiquitin-specific protease that removes ubiquitin-specific protease activity   cytoplasm UBP1                     1
YNL194C   YNL194C                         906 unknown           unknown                  E, C       cytoplasm YNL194C                  4
                    Integral membrane protein localized to eisosomes, large immobile protein structures at the cell cortex associated with endocytos
YJL080C   SCP160    Essential RNA-binding G protein effectorRNA binding
                                         3669 chromosome segregationresponse pathway, mainly associated with nuclear envelope and ER, interact
                                                                 of mating               E, R       ER          SCP160                 0
YEL043W   YEL043W                        2871 unknown
                    Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction map
                                                                unknown                  E, R       ER          YEL043W                0
YKL065C   YET1                            621 unknown
                    Endoplasmic reticulum transmembrane protein; may interact withR
                                                                unknown                             ER          YET1                   3
                                                                                         E, ribosomes, based on co-purification experiments; homolog of h
YLL023C   YLL023C                         840 unknown
                    Protein of unknown function; may interact with ribosomes, based R co-purification YLL023C
                                                                unknown                  E, on      ER                                 5
                                                                                                                 experiments; green fluorescent protein (GFP)
YOR175C   YOR175C                        1860 unknown
                    Protein of unknown function that may interact with ribosomes, E, R on co-purification experiments; member of the MBOAT fam
                                                                O-acyltransferase activity
                                                                                         based      ER          YOR175C                7
YGR261C   APL6      Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate path
                                         2430 vesicle-mediated transport
                                                                unknown                  G                      APL6
                                                                                                    Golgi, endosome?                   0
YPL259C   APM1      Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi p
                                         1428 vesicle-mediated transport
                                                                clathrin binding         G                      APM1
                                                                                                    Golgi, endosome?                   0
YHL019C   APM2      Protein of unknown function, homologous totransport
                                         1818 vesicle-mediated the medium chain of mammalian clathrin-associated protein 0
                                                                clathrin binding         G                      APM2
                                                                                                    Golgi, endosome?                   complex; involved in ve
YKR068C   BET3      Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; compo
                                          582 ER to Golgi transport
                                                                unknown                  G          Golgi       BET3                   0
YML077W   BET5      Component of the TRAPPER to Golgiprotein particle) complex,G
                                          480 (transport transport
                                                                unknown                             TRAPP complex
                                                                                                                BET5                   0
                                                                                           which plays an essential role in the vesicular transport from endop
YDR270W   CCC2      Cu(+2)-transporting 3015 iron ion homeostasis export of copper from the cytosol into an extracytosolic compartment; has simila
                                         P-type ATPase, required for
                                                                cation-transporting ATPase activity
                                                                                         G          Golgi       CCC2                   8
YGL223C   COG1      Essential component of the conserved oligomeric Golgi complex (Cog1p Golgi
                                         1254 intra-Golgi transport
                                                                unknown                  G                      COG1                   0
                                                                                                     through Cog8p), a cytosolic tethering complex that func
YNL041C   COG6      Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in pr
                                         2520 intra-Golgi transport
                                                                unknown                  G          Golgi       COG6                   0
YML071C   COG8      Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in pr
                                         1824 intra-Golgi transport
                                                                unknown                  G          Golgi       COG8                   0
YDL145C   COP1      Alpha subunit of COPI vesicleto Golgi transport which surrounds transport vesicles in the early secretory pathway
                                         3606 ER coatomer unknown
                                                                complex,                 G                      COP1
                                                                                                    COPI vesicle coat                  0
YKL179C   COY1      Golgi membrane protein with similarity tounknown
                                         2040 Golgi vesicle transport
                                                                 mammalian CASP; genetic interactions with GOS1 (encoding a1
                                                                                         G          Golgi       COY1                    Golgi snare protein) su
YMR162C   DNF3                           4971 intracellular protein maintains
                                                                phospholipid-translocating lipid Golgi
                                                                                         G           asymmetry in post-Golgi secretory vessicles; localizes t
                    Aminophospholipid translocase (flippase) thattransport membraneATPase activityDNF3                                 8
YGR284C   ERV29     Protein localized to COPII-coated vesicles, involved in vesicle G
                                          933 ER to Golgi transport
                                                                unknown                             COPII-coated vesicle
                                                                                                                ERV29                  7
                                                                                         formation and incorporation of specific secretory cargo
YDR373W   FRQ1                            573 regulation of signal transduction
                                                                enzyme have a role in intracellular signaling through its regulation of the phosphatidylino
                    N-myristoylated calcium-binding protein that may activator activity  G          membraneFRQ1                       0
YEL042W   GDA1      Guanosine diphosphataseprotein amino acid glycosylation theG activity GDP-mannose into the Golgi lumen by converting GD
                                         1557 located in the guanosine-diphosphatase
                                                                Golgi, involved in                  Golgi
                                                                                          transport of          GDA1                   1
YJR031C   GEA1      Guanine nucleotide 4227 ER to Golgi transport
                                         exchange factor for ADP ribosylation factorsexchange factor activity
                                                                ARF guanyl-nucleotide (ARFs),Golgi
                                                                                         G                      GEA1                   0
                                                                                                     involved in vesicular transport between the Golgi and E
YEL022W   GEA2      Guanine nucleotide 4380 ER to Golgi transport
                                         exchange factor for ADP ribosylation factorsexchange factor activity
                                                                ARF guanyl-nucleotide (ARFs),Golgi
                                                                                         G                      GEA2                   0
                                                                                                     involved in vesicular transport between the Golgi and E
YHR108W   GGA2      Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent mann
                                         1758 Golgi to vacuole transport
                                                                unknown                  G          Golgi       GGA2                   0
YER122C   GLO3                           1482 ER to activating protein
                                                                ARF GTPase activator activity COPI-coated vesicle
                                                                                         G                      GLO3                   0
                    ADP-ribosylation factor GTPase Golgi transport (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gc
YJR075W   HOC1                           1191 cell wall mannoprotein biosynthesis G
                                                                in cell wall activity, transferring alpha0,6-mannosyltransferase complex
                                                                                                     glycosyl of a Golgi-localized complex that also contains
                    Alpha-1,6-mannosyltransferase involved transferasemannan biosynthesis; subunitgroups        HOC1                   1
YNL029C   KTR5                           1569 cell involved inmannosyltransferase Gmember of the
                                                                 protein glycosylation;                         KTR5
                    Putative mannosyltransferase wall organization and biogenesisactivity Golgi KRE2/MNT1 mannosyltransferase family   1
YIL085C   KTR7                           1554 cell involved inmannosyltransferase Gmember of the
                                                                 protein glycosylation;                         KTR7
                    Putative mannosyltransferase wall organization and biogenesisactivity Golgi KRE2/MNT1 mannosyltransferase family   1
YER001W     MNN1      Alpha-1,3-mannosyltransferase, integral acid N-linked glycosylation the Golgi complex, required for addition of alpha1,3-mannose
                                           2289 protein amino alpha0,3-mannosyltransferase activity
                                                                    membrane glycoprotein ofG           Golgi        MNN1                   1
YPL050C     MNN9                           1188 protein amino acid N-linked glycosylation
                                                                    mannosyltransferase G    Anp1p, membraneMNN9
                      Subunit of Golgi mannosyltransferase complex also containingactivity Mnn10p, Mnn11p, and Hoc1p that1mediates elongation of
YGL038C     OCH1      Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of
                                           1443 protein amino acid N-linked glycosylation
                                                                    transferase activity, transferring Golgi
                                                                                            G            glycosyl groups
                                                                                                                     OCH1                   1
YFR051C     RET2      Delta subunit of the 1641 ER to Golgi transport which coats Golgi-derived transport RET2
                                           coatomer complex (COPI), binding
                                                                    protein                 G                         vesicles;             0
                                                                                                        COPI vesicle coat involved in retrograde transport betw
YPL010W     RET3                             570 retrograde transport, Golgi coats G
                                                                    protein binding                       transport vesicles;
                                                                                                                     RET3                   0
                      Zeta subunit of the coatomer complex (COPI), which to ER Golgi-derivedCOPI vesicle coat involved in retrograde transport betw
YIL109C     SEC24     Component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle vesicle coat in ER to Golgi transport, cargo sele
                                           2781 ER to Golgi transport binding
                                                                    protein                 G                        SEC24
                                                                                                        COPII coat; involved                0
YDR238C     SEC26     Essential beta-coat protein of to Golgi transport
                                           2922 ER the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morp
                                                                    unknown                 G                        SEC26
                                                                                                        COPI vesicle coat                   0
YGL137W     SEC27     Essential beta'-coat 2870 ER to Golgi transport
                                            protein of the COPI coatomer, involved in G
                                                                    unknown                             COPI Golgi-to-ER
                                                                                                                     SEC27                  0
                                                                                            ER-to-Golgi andvesicle coat transport; contains WD40 domains th
YDL195W     SEC31                          3822 component transport
                      Essential phosphoprotein ER to Golgi (p150) of themolecule activity
                                                                    structural COPII coatG secretory pathway vesicles, in complex 0
                                                                                             of         COPII vesicle coat
                                                                                                                     SEC31                   with Sec13p; required f
YLR026C     SED5                           1023 ER to Golgi transport activity
                                                                    t-SNARE                 G           Golgi        SED5
                      cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds1at least 9 SNARE prot
YNL049C     SFB2                           2631 ER coated vesicles  unknown                 G           COPII vesicle coat
                      Probable component of COPIIto Golgi transportthat binds to Sec23p; similar to and SFB2         functionally redundant0with Sec24p, but expre
YKL006C-A   SFT1                              required for transport of
                                                                    v-SNARE activity        G            and a        Golgi
                      Intra-Golgi v-SNARE,435 intra-Golgi transport proteins between an early Golgi laterSFT1 compartment 1
YOR219C     STE13                          2796 peptide pheromone maturation
                                                                      membrane protein that G             on the STE13                      1
                      Dipeptidyl aminopeptidase, Golgi integralaminopeptidase activity cleavesGolgi carboxyl side of repeating -X-Ala- sequences, re
YDR246W     TRS23     One of 10 subunits of660 transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion
                                              the ER to Golgi transport
                                                                    unknown                 G           TRAPP complexTRS23                  0
YDR472W     TRS31     One of 10 subunits of852 transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion
                                              the ER to Golgi transport
                                                                    unknown                 G           TRAPP complexTRS31                  0
YOR115C     TRS33     One of 10 subunits of807 transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion
                                              the ER to Golgi transport
                                                                    unknown                 G           TRAPP complexTRS33                  0
YKL034W     TUL1                           2277 protein ubiquitination
                                                                    ubiquitin-protein in ubiquitinating and
                                                                                            G                        TUL1                   8
                      Golgi-localized RING-finger ubiquitin ligase (E3), involvedligase activity Golgi sorting membrane proteins that contain polar tran
YDR100W     TVP15                            432 unknown
                      Integral membrane protein localized to late Golgi vesicles along with the Golgi
                                                                    unknown                 G           v-SNARE Tlg2pTVP15                  3
YGL161C     YIP5                             with Rab GTPases;Rab GTPase analysis
                                                                      computational                     membraneYIP5
                      Protein that interacts 933 vesicle-mediated transport binding G of large-scale protein-protein interaction4            data suggests a possib
YER005W     YND1      Apyrase with wide substrate specificity, involved in preventing G inhibition of glycosylation by hydrolyzing nucleoside tri- and dip
                                           1893 protein amino acid glycosylation
                                                                    nucleoside-diphosphatase activity
                                                                                            the         Golgi        YND1                   1
YLR262C     YPT6      GTPase, Ras-like GTP binding proteinprotein transportsecretory pathway, required for fusion of endosome-derived vesicles with t
                                             648 intracellular involved inactivity
                                                                    GTPase the              G           Golgi        YPT6                   0
YDL137W     ARF2      ADP-ribosylation factor, GTPaseGolgi transport activityinvolved C regulation of coated formation vesicles in intracellular traffick
                                             546 ER to of the Ras superfamily
                                                                    GTPase                  G, in       cytosol      ARF2                   0
YBR164C     ARL1      Soluble GTPase with 552 protein targeting to vacuole traffic; regulates potassium influx; G protein of the0
                                             a role in regulation GTPase activity
                                                                    of membrane             G, C        Golgi        ARL1                    Ras superfamily, simila
YLR330W     CHS5                           2016 spore wall assembly to plasma               G, C        cytoplasm CHS5                      role
                      Protein involved in export from the Golgi unknown membrane; involved in chitin biosynthesis through its 0 in Chs3p localizatio
YGR120C     COG2      Essential component 789 ER to Golgi transport binding complex (Cog1p soluble fraction a cytosolic tethering complex that func
                                             of the conserved oligomeric Golgi
                                                                    protein                 G, C                     COG2
                                                                                                         through Cog8p),                    0
YDR517W     GRH1                           1119 mitotic spindle checkpoint;
                                                                     checkpoint             of C        cytoplasm which                     0
                      Protein involved in the spindle assembly unknown homolog G, human GRASP65,GRH1 is a Golgi localized protein that functio
YPL145C     KES1                           1305 vesicle-mediated transport
                                                                    oxysterol binding        seven      cytoplasm KES1                      0
                      Member of the oxysterol binding protein family, which includesG, C yeast homologs; involved in negative regulation of Sec14p-
YNL183C     NPR1                           2373 regulation plasma membrane amino acid transporters byNPR1
                                                                    kinase activity
                      Protein kinase that stabilizes severalof nitrogen utilization         G, C                                            0
                                                                                                        cytoplasm antagonizing their ubiquitin-mediated degrad
YMR079W     SEC14                          1071 sporulation
                      Phosphatidylinositol/phosphatidylcholine phosphatidylinositol transporter cytosolregulation of PtdIns and PtdCho metabolism, pro
                                                                     transfer protein involvedC coordinate
                                                                                            G, in       activity     SEC14                  0
YDR084C     TVP23                            600 unknown            unknown                 G, C        v-SNARE TVP23                       3
                      Integral membrane protein localized to late Golgi vesicles along with the cytoplasm Tlg2p; green fluorescent protein (GFP)-fusion
YKR088C     TVP38                          1014 unknown             unknown                 G, C        v-SNARE TVP38                       5
                      Integral membrane protein localized to late Golgi vesicles along with the cytoplasm Tlg2p; green fluorescent protein (GFP)-fusion
YGL095C     VPS45     Protein of the Sec1p/Munc-18 family, essential forprotein binding C sorting; required for the function of Pep12p and the early end
                                           1734 protein complex assembly
                                                                    unfolded vacuolar protein
                                                                                            G,          cytosol      VPS45                  0
YJL029C     VPS53                          2469 Golgi to vacuole retrograde
                                                                    protein binding         G, C
                      Component of the GARP (Golgi-associatedtransport protein) complex, cytoplasm VPS53                                    0
                                                                                                         Vps51p-Vps52p-Vps53p-Vps54p, which is required for t
YPL085W     SEC16     COPII vesicle coat proteinvesicle-mediatedtransport vesicle budding and ER
                                           6588 required for ER transport
                                                                    structural molecule activity
                                                                                            G, E                     SEC16                  0
                                                                                                         autophagosome formation; Sec16p is bound to the perip
YLR268W     SEC22                            645 ER into SNARE complex with Bet1p, Bos1p and Sed5p; SEC22between the ER and Golgi complex; in
                      R-SNARE protein; assemblesto Golgi transport  v-SNARE activity        G, E        ER, golgi cycles                    1
YHR098C     SFB3                           2790 ER forms a complex,
                      Member of the Sec24p family;to Golgi transport with Sec23p, G, E is involved in sorting of Pma1p into COPII vesicles; peripheral
                                                                    unknown                 that        ER           SFB3                   0
YDR189W     SLY1      Hydrophilic protein involved into Golgi trafficking between the ER E Golgi; SM (Sec1/Munc-18) family protein that binds the tSN
                                           2001 ER vesicle transport binding
                                                                    SNARE                   G, and      ER           SLY1                   0
YGR172C     YIP1      Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; lo
                                             747 ER to Golgi transport
                                                                    unknown                 G, E        ER           YIP1                   5
YAL026C     DRS2      Aminophospholipid translocase (flippase) that maintains membrane lipid PM
                                           4068 processing of 20S pre-rRNA
                                                                    ATPase activity         G, P                     DRS2                   9
                                                                                                         asymmetry in post-Golgi secretory vessicles; localizes t
YMR237W     BCH1                           2175 unknown
                      Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group ofR related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p)
                                                                    unknown                 G, 4                     BCH1
                                                                                                        clathrin-coated vesicle             0
YJL207C     YJL207C                        6045 unknown
                      AP-1 accessory protein; colocalizes with unknown the late-Golgi R
                                                                     clathrin to                        clathrin-coated TGN-endosome transport; physically inte
                                                                                                                     YJL207C
                                                                                            G, apparatus; involved in vesicle               0
YJL123C     YJL123C                        1437 unknown             unknown                 green fluorescent protein (GFP)-fusion protein localizes to the cyto
                      Protein of unknown function that may interact with ribosomes; G, R, C cytoplasm YJL123C                               0
YBR042C     YBR042C                                                 acyltransferase activity
                      Hypothetical protein1194 phospholipid biosynthesis                    L                        Y
                                                                                                        lipid particle BR042C               4
YDL052C     SLC1      1-acyl-sn-gylcerol-3-phosphate acyltransferase, catalyzes the L
                                             912 sphingolipid biosynthesis
                                                                    1-acylglycerol-3-phosphate O-acyltransferase activity to form phosphatidic acid, a key
                                                                                            acylation of lysophosphatidic acid
                                                                                                        lipid particle LC1
                                                                                                                     S                      1
YLL012W     YEH1                           1722 sterol metabolism     products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and invol
                      Steryl ester hydrolase, one of three genesterol esterase activity     L           membraneYEH1                        1
YMR313C     TGL3                           1929 lipid metabolism
                      Triacylglycerol lipase of the lipid particle, triacylglycerol lipase activity lipase activity ofGL3 lipid particle; contains the consensus se
                                                                     responsible for all the TAG
                                                                                            L                        T
                                                                                                        lipid particle the                  1
YOR059C     YOR059C   Hypothetical protein1353 unknown              unknown                 L                        Y
                                                                                                        lipid particle OR059C               1
YCL005W     LDB16                            771 unknown
                      Protein of unknown function; null mutant unknown
                                                                    cells display decreased M negative charge of the cell surface; the authentic, non-tagge
                                                                                            L, net      Mito         LDB16                  2
Q0080       AAP1      Subunit 8 of the F0 sectorATP synthesis hydrogen-transporting ATP synthase activity, rotational mechanism
                                             147 of mitochondrial inner membrane F1-F0 ATP synthase, encoded on the mitochondrial genome
                                                                     coupled proton transport
                                                                                            M           Mito         AAP1                   1
Q0085       ATP6                             780 protein complex assembly mitochondrial
                                                                     F0 sector of           M           Mito         ATP6
                      Mitochondrially encoded subunit 6 of the structural molecule activity F1F0 ATP synthase, which is a large,6evolutionarily conserve
Q0105       COB                            7108 aerobic respiration ubiquinol-cytochrome-c reductase activity COB
                                                                                            M           Mito                                9
                      Cytochrome b, mitochondrially encoded subunit of the ubiquinol-cytochrome c reductase complex which includes Cobp, Rip1p, C
Q0115       BI3       Mitochondrial mRNA maturase, forms a complex with Mrs1p to mediate splicing of the bI3 intron of the COB gene; encoded by b
                                           3726 Group I intron splicing
                                                                    RNA binding             M           Mito         BI3                    6
Q0130       OLI1      F0-ATP synthase subunit protein complex assembly
                                             231 9 (ATPase-associated proteolipid),activity
                                                                    structural molecule encoded on the mitochondrial genome; mutation confers oligomycin
                                                                                            M           Mito         OLI1                   2
Q0275       COX3                             810 aerobic respirationcytochrome-c oxidase activity mitochondrial inner membrane electron transport chain;
                                                                                            M
                      Subunit III of cytochrome c oxidase, which is the terminal member of theMito                   COX3                   7
YAL008W     FUN14                            of unknown function
                      Mitochondrial protein 597 unknown             unknown                 M           Mito         FUN14                  2
YAL010C     MDM10     Subunit of the mitochondrial sorting and assembly machinery (SAM complex); has a role in assembly of the TOM complex, which
                                           1482 protein complex assembly
                                                                    unknown                 M           Mito         MDM10                  0
YAL048C     GEM1      Evolutionarily-conserved tail-anchored outertransport
                                           1989 vesicle-mediated mitochondrial membrane GTPase which regulates mitochondrial morphology; cells l
                                                                    GTPase activity         M           Mito         GEM1                   1
YAR035W     YAT1      Outer mitochondrial 2064 alcohol metabolism minor ethanol-inducible enzyme involved in transport of activated acyl groups fro
                                            carnitine acetyltransferase, O-acetyltransferase activity
                                                                    carnitine               M           Mito         YAT1                   0
YBL013W     FMT1                           1206 translational initiation
                                                                    methionyl-tRNA formyltransferase activity mitochondria; potential Cdc28p substrate
                                                                                            M           Mito
                      Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA inFMT1                              0
YBL059W     YBL059W                          651 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YBL059W
                                                                   authentic,            M         Mito                                2
                                                                                                                in highly purified mitochondria in high-through
YBL080C     PET112                          1626 protein translation;
                                                                 unknown                 M         Mito        PET112
                      Protein required for mitochondrial biosynthesismutation is functionally complemented by a Bacillus subtilis0ortholog
YBL095W     YBL095W                          813 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YBL095W
                                                                   authentic,            M         Mito                                0
                                                                                                                in highly purified mitochondria in high-through
YBR024W     SCO2                             906 copper ion inner membrane, similar to Sco1p Mito may have a redundant function with Sco1p in delive
                                                                 thioredoxin peroxidase activity and
                      Protein anchored to the mitochondrialtransport                     M                     SCO2                    0
YBR037C     SCO1                             888 the mitochondrial inner membrane, required Mito
                                                                 thioredoxin             M                     SCO1                    0
                      Copper-binding protein of protein complex assembly peroxidase activityfor cytochrome c oxidase activity and respiration; may fun
YBR044C     TCM62     Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone
                                            1722 protein complex assembly
                                                                 unfolded protein bindingM         Mito        TCM62                   0
YBR047W     FMP23                            528 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP23 purified mitochondria in high-through
                                                                   authentic,            M         Mito         in highly              0
YBR054W     YRO2                            1035 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YRO2 purified mitochondria in high-through
                                                                   authentic,            M         Mito         in highly              7
YBR091C     MRS5                             330 mitochondrial inner membrane protein import Mito
                                                                 protein transporter activity
                                                                                         M                     MRS5                    0
                      Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-p
YBR104W     YMC2                             990 transport       transporter member M              Mito        YMC2
                      Putative mitochondrial inner membrane transporter, activity of the mitochondrial carrier (MCF) family 0
YBR147W     YBR147W                          891 unknown           authentic,            M         unknown in highly purified mitochondria in high-through
                      Putative protein of unknown function; theunknown non-tagged protein is detected YBR147W                          7
YBR176W     ECM31     Ketopantoate hydroxymethyltransferase, 3-methyl-2-oxobutanoateacid biosynthesis,ECM31 activity
                                             939 pantothenate biosynthesis pantothenic hydroxymethyltransferase 2-oxoisovalerate into 2-dehydropan
                                                                  required for           M         Mito          converts              0
YBR179C     FZO1      Mitochondrial integral membrane proteinorganizationmitochondrial fusionMito maintenance of the mitochondrial genome; contain
                                            2568 mitochondrial GTPase activity biogenesis
                                                                  involved in and        M          and        FZO1                    0
YBR192W     RIM2                            1134 mitochondrial genome maintenance M
                                                                 transporter activity              Mito        RIM2
                      Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports 0        pyrimidine nucleoside m
YBR203W     COS111                          2775 signal the antifungal
                                                                 unknown
                      Protein required for resistance totransduction drug ciclopiroxM              unknown to the subtelomerically-encoded COS family;
                                                                                          olamine; not related COS111                  0
YBR262C     FMP51                            321 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP51 purified mitochondria in high-through
                                                                   authentic,            M         Mito         in highly              0
YBR291C     CTP1      Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family
                                             900 mitochondrial citrate transport
                                                                 tricarboxylate carrier activity
                                                                                         M         Mito        CTP1                    0
YCR024C     SLM5                            1479 protein biosynthesis
                      Mitochondrial asparaginyl-tRNA synthetase                          M
                                                                 asparagine-tRNA ligase activity Mito          SLM5                    0
YDL027C     YDL027C                         1263 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YDL027C
                                                                   authentic,            M         Mito                                2
                                                                                                                in highly purified mitochondria in high-through
YDL029W     ARP2      Essential component of the Arp2/3 complex, whichconstituent M cytoskeleton nucleation center required 0 the motility and inte
                                            1299 actin filament organization a highly conservedMito
                                                                 structural is           of         actin      ARP2                     for
YDL044C     MTF2      Mitochondrial matrix1323 proteininteracts with binding
                                             protein that biosynthesis an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RN
                                                                 RNA                     M         Mito        MTF2                    0
YDL067C     COX9                             180 aerobic respiration
                                                                 cytochrome-c oxidase activity Mito
                                                                                         M                     COX9                    1
                      Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport cha
YDL085W     NDE2                            1638 ethanol fermentation dehydrogenase activity cytosolic NADH; Nde1p and Nde2p are involved in provi
                                                                 NADH                    M
                      Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of Mito               NDE2                    0
YDL104C     QRI7                            1224 similar to O-sialoglycoprotein metallopeptidase from P. haemolytica; the authentic, non-tagged protein
                      Putative metalloprotease, proteolysis                              M
                                                                 metalloendopeptidase activity Mito            QRI7                    0
YDL119C     YDL119C                          924 transport       transporter activity M            Mito        YDL119C                 0
                      Putative mitochondrial transport protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throu
YDL120W     YFH1      Frataxin, regulates mitochondrial iron accumulation; interacts with Isu1p Mito promotes Fe-S cluster assembly; interacts with el
                                             525 iron ion homeostasis regulator activity
                                                                 enzyme                  M         which       YFH1                    0
YDL130W-A   STF1      Protein involved in regulation ofsynthesis unknownproton transport
                                             261 ATP the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing 0
                                                                  coupled                M         mito        STF1                     factors that enhance inh
YDL142C     CRD1      Cardiolipin synthase; 852 mitochondrial organization and biogenesis
                                              produces cardiolipin, which is synthase activity
                                                                 cardiolipin an important constituent of mitochondrial membranes; required for normal mi
                                                                                         M         Mito        CRD1                    3
YDL157C     YDL157C                          357 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YDL157C
                                                                   authentic,            M         Mito                                0
                                                                                                                in highly purified mitochondria in high-through
YDL181W     INH1      Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase, inhibitorymito
                                             258 ATP synthesis enzyme inhibitortransport
                                                                  coupled proton activityM                     INH1                    0
                                                                                                    function is enhanced by stabilizing proteins Stf1p and S
YDL203C     YDL203C                         1872 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YDL203C
                                                                   authentic,            M         Mito                                0
                                                                                                                in highly purified mitochondria in high-through
YDL217C     TIM22                            624 mitochondrial inner membrane protein import Mito
                                                                 protein complex involvedM                     TIM22                   2
                      Component of the mitochondrial Tim54p-Tim22ptransporter activity in insertion of polytopic proteins into the inner membrane
YDL222C     FMP45     Integral membrane protein localized to mitochondriabiogenesis protein) and eisosomes, immobile patches at the cortex associate
                                             930 cell wall organization and (untagged
                                                                 unknown                 M         Mito        FMP45                   4
YDL248W     COS7                            1152 unknown
                      Protein of unknown function, member of receptor activity
                                                                 the DUP380 subfamily of conserved, oftenCOS7
                                                                                         M         Mito                                4
                                                                                                                 subtelomerically-encoded proteins; the authe
YDR044W     HEM13     Coproporphyrinogen III oxidase, biosynthesis
                                             987 heme an oxygen requiring enzyme oxidase activity sixth step in the heme biosynthetic pathway; loca
                                                                 coproporphyrinogen that catalyzes the
                                                                                         M         Mito        HEM13                   0
YDR065W     YDR065W                         1098 unknown
                      Protein of unknown function, required forunknown acidification; the authentic, non-tagged protein is detected in highly purified mi
                                                                   vacuolar              M         Mito        YDR065W                 0
YDR070C     FMP16                            282 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP16 purified mitochondria in high-through
                                                                   authentic,            M         Mito         in highly              0
YDR079W     PET100    Chaperone that specifically facilitates theunfolded protein binding c oxidase, integral to the mitochondrial inner membrane; inter
                                             336 aerobic respiration
                                                                   assembly of cytochromeM         Mito        PET100                  0
YDR178W     SDH4      Membrane anchor subunittricarboxylic acid cycle dehydrogenase (ubiquinone) Sdh4p), which couples the oxidation of succinate
                                             546 of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, activity
                                                                 succinate               M         Mito        SDH4                    2
YDR197W     CBS2      Mitochondrial translationalprotein biosynthesis mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untransla
                                            1170 activator of the COB
                                                                 unknown                 M         Mito        CBS2                    0
YDR204W     COQ4      Protein with a role in1008 ubiquinone metabolism
                                             ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix
                                                                 unknown                 M         Mito        COQ4                    0
YDR219C     YDR219C   Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial complex
                                            1398 ubiquitin-dependent protein catabolism
                                                                 protein binding         M                     YDR219C                 0
                                                                                                   ubiquitin ligase morphology; interacts with Skp1p throug
YDR231C     COX20     Mitochondrial inner membrane protein, required for proteolytic M
                                             618 aerobic respiration
                                                                 unfolded protein binding          Mito          and its
                                                                                         processing of Cox2p COX20 assembly into0        cytochrome c oxidase
YDR236C     FMN1                             657 FMN biosynthesis
                      Riboflavin kinase, phosphorylates riboflavin to form riboflavin monophosphate (FMN), which is a necessary cofactor for many enz
                                                                 riboflavin kinase activity
                                                                                         M         Mito        FMN1                    1
YDR326C     YDR326C                         4317 unknown         unknown                 M         unknown YDR326C                     1
                      Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidifica
YDR375C     BCS1      Protein of the mitochondrial inner membrane that activity as M ATP-dependent chaperone, required for the assembly of the cy
                                            1371 aerobic respiration
                                                                 ATPase functions        an        Mito        BCS1                    1
YDR377W     ATP17     Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase,ATP synthase activity, rotational mechanism
                                             306 ATP synthesis hydrogen-transporting which is Mito
                                                                  coupled proton transport
                                                                                         M                     ATP17
                                                                                                   a large, evolutionarily conserved0    enzyme complex requi
YDR393W     SHE9      Mitochondrial inner membrane protein required for normalbiogenesis
                                            1371 mitochondrial organization and mitochondrial Mito
                                                                 unknown                 M                     SHE9
                                                                                                   morphology, may be involved in 1    fission of the inner mem
YDR470C     UGO1                            1509 transport       transporter activity M            Mito        UGO1                    3
                      Protein of unknown function; outer membrane component of the mitochondrial fusion machinery; Ugo1p bind directly to Fzo1p an
YDR493W     FMP36                            372 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected FMP36 purified mitochondria in high-through
                                                                   authentic,            M         Mito         in highly              0
YDR511W     ACN9                             402 gluconeogenesis
                      Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher e
                                                                 unknown                 M         Mito        ACN9                    0
YDR538W     PAD1                             729 aromatic compound catabolism
                                                                 carboxy-lyase cinnamic            Mito        PAD1                    0
                      Phenylacrylic acid decarboxylase, confers resistance toactivityM acid, decarboxylates aromatic carboxylic acids to the correspon
YEL030W     ECM10     Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with M
                                            1935 protein targeting to Mito
                                                                 unknown                 M         Mito        ECM10                   0
YEL039C     CYC7      Cytochrome c isoform 2, expressed under hypoxic conditions; M
                                             342 electron transport
                                                                 electron carrier activity
                                                                                         electron carrier of the mitochondrial intermembrane space that tra
                                                                                                   Mito        CYC7                    0
YER053C     PIC2                             903 phosphate transport
                                                                  inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abund
                                                                                         M
                      Mitochondrial phosphate carrier, imports inorganic phosphate transporterMito  activity PIC2                      1
YER058W     PET117    Protein required for assembly of cytochrome c oxidase assembly
                                             324 cytochrome c oxidase complex
                                                                 unknown                 M         Mito        PET117                  1
YER061C     CEM1      Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial0
                                            1329 fatty acid biosynthesis synthase activity
                                                                 fatty-acid              M         Mito        CEM1                      respiration
YER076C     YER076C                          909 unknown
                      Putative protein of unknown function; theunknown non-tagged protein is detected YER076C
                                                                   authentic,            M         Mito                                0
                                                                                                                in highly purified mitochondria in high-through
YER077C     YER077C   Hypothetical protein2067 unknown           unknown                 M         Mito        YER077C                 0
YER140W     YER140W                           1671 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YER140W
                                                                      authentic,           M          Mito                               5
                                                                                                                 in highly purified mitochondria in high-through
YER141W     COX15       Protein required for the hydroxylation of heme O complexhemeM acting on an essential prosthetic group for cytochrome c oxidase
                                              1461 cytochrome c oxidase to form activity, which is NADH or NADPH, heme protein as acceptor
                                                                    oxidoreductase assembly A,        Mito       COX15                   8
YER153C     PET122      Specific translational 765 protein biosynthesis
                                               activator for the COX3 mRNA that acts together with Pet54p and Pet494p; located in the mitochondrial inne
                                                                    translation regulator activity
                                                                                           M          Mito       PET122                  0
YER170W     ADK2        Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; 0
                                               678 nucleotide metabolism kinase activity
                                                                    adenylate              M          Mito       ADK2                     may serve as a back-u
YER183C     FAU1        5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis activity
                                               636 folic acid and derivative biosynthesis cyclo-ligase
                                                                    5-formyltetrahydrofolate
                                                                                           M          Mito       FAU1                    0
YFL030W     AGX1                              aminotransferase, catalyzes
                                                                    alanine-glyoxylate transaminase from
                                                                                            of          activity AGX1
                        Alanine : glyoxylate 1158 glycine biosynthesis the synthesisM glycine Mito glyoxylate, which is one of 0         three pathways for glyc
YFL036W     RPO41                             4056 mitochondrial genome maintenance M those of T3
                                                                    DNA-directed similar to           activity   RPO41                   0
                        Mitochondrial RNA polymerase; single subunit enzymeRNA polymerase Mito and T7 bacteriophages; requires a specificity subun
YFL046W     FMP32                              624 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP32 purified mitochondria in high-through
                                                                      authentic,           M          Mito       in highly               1
YFR033C     QCR6                               444 cytochrome-c reductase complex, which is a component QCR6mitochondrial inner membrane electron
                                                                    ubiquinol-cytochrome-c reductase activity of the
                        Subunit 6 of the ubiquinol aerobic respiration                     M          Mito                               0
YGL002W     ERP6        Protein with similarity651 secretory pathway member of the p24 family Mito
                                                to Emp24p and Erv25p,
                                                                    unknown                M                      ER to                  2
                                                                                                      involved inERP6 Golgi transport; the authentic, non-tag
YGL057C     YGL057C                            864 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YGL057C
                                                                      authentic,           M          Mito                               0
                                                                                                                 in highly purified mitochondria in high-through
YGL059W     YGL059W                           1476 unknown          protein kinase activityM          Mito       YGL059W                 0
                        Protein kinase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL064C     MRH4                              1686 ribosome biogenesis
                                                                    RNA helicase activityM            Mito       MRH4
                        Mitochondrial RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function             0
YGL104C     VPS73       Mitochondrial protein of unknown targetinginvolved in vacuolarM
                                              1461 protein function to vacuole
                                                                    unknown                           Mito
                                                                                            protein sorting      VPS73                 10
YGL191W     COX13                              390 aerobic respiration
                                                                    enzyme regulator memberM          Mito       COX13
                        Subunit VIa of cytochrome c oxidase, which is the terminal activity of the mitochondrial inner membrane1electron transport chai
YGL236C     MTO1        Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of th
                                              2010 protein biosynthesis
                                                                    unknown                M          Mito       MTO1                    0
YGL256W     ADH4                              1398 fermentation alcohol demonstrated activity, Mito
                                                                                           M          zinc-dependent
                                                                                                                 ADH4                    0
                        Alcohol dehydrogenase type IV, dimeric enzymedehydrogenaseto be zinc-dependent despite sequence similarity to iron-activated
YGR008C     STF2        Protein involved in regulation ofsynthesis unknownproton transport
                                               255 ATP the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing 0
                                                                     coupled               M          mito       STF2                    factors that enhance inh
YGR021W     YGR021W                            873 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YGR021W
                                                                      authentic,           M          Mito                               0
                                                                                                                 in highly purified mitochondria in high-through
YGR028W     MSP1        Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase
                                              1089 protein targeting to Mitoactivity
                                                                    ATPase                 M          Mito       MSP1                    0
YGR033C     TIM21       Constituent of the mitochondrial inner membrane presequence translocase (TIM23 TIM21
                                               720 mitochondral protein import
                                                                    unknown                M          Mito                               1
                                                                                                                 complex); may regulate protein import by bind
YGR102C     YGR102C                            552 unknown
                        Putative protein of unknown function; transposon insertion mutant is salt Mito
                                                                    unknown                M                     YGR102C                 0
                                                                                                      sensitive and deletion mutant has growth defects; the au
YGR112W     SHY1                              1170 aerobic respiration
                                                                    unfolded normal respiration, possible chaperone involved in assembly of cytochrome c
                        Mitochondrial inner membrane protein required forprotein binding   M          membraneSHY1                       2
YGR150C     YGR150C                           2595 unknown
                        Protein of unknown function, contains PPR motifs; mutant hasM
                                                                    unknown                           Mito       YGR150C                 0
                                                                                            growth defects on both non-fermentable carbon sources and rich
YGR171C     MSM1                              1728 methionyl-tRNA aminoacylation ligase activity Mito
                                                                    methionine-tRNA        M                     MSM1                    0
                        Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarl
YGR174C     CBP4        Mitochondrial protein 513 protein complex assembly
                                                required for assembly of ubiquinol cytochrome-c reductase complex (cytochrome bc1 complex); interacts w
                                                                    unknown                M          Mito       CBP4                    1
YGR183C     QCR9                               414 cytochrome-c reductase complex, which is a component QCR9mitochondrial inner membrane electron
                                                                    ubiquinol-cytochrome-c reductase activity of the
                        Subunit 9 of the ubiquinol aerobic respiration                     M          Mito                               1
YGR222W     PET54                              882 protein COX1 RNA AI5 beta; also specifically required, PET54 with Pet122p0and Pet494p, for trans
                                                                     intron
                        Protein required for splicing of thebiosynthesis binding           M          Mito       together
YGR235C     YGR235C                            702 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YGR235C
                                                                      authentic,           M          Mito                               1
                                                                                                                 in highly purified mitochondria in high-through
YGR236C     SPG1                               390 unknown
                        Protein required for survival at high temperature during stationary phase;Mito required for growth on nonfermentable carbon sourc
                                                                    unknown                M           not       SPG1                    1
YGR243W     FMP43                              441 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP43 purified mitochondria in high-through
                                                                      authentic,           M          Mito       in highly               0
YGR286C     BIO2        Biotin synthase, catalyzesbiotin biosynthesis dethiobiotin activity which is the last step of the biotin biosynthesis pathway; comp
                                              1128 the conversion of synthase to biotin,
                                                                    biotin                 M          Mito       BIO2                    0
YHL014C     YLF2                              1218 unknown
                        Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detecte
                                                                    GTP binding            M          Mito       YLF2                    0
YHR002W     LEU5                               protein involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease
                                                                    coenzyme A transporter activity Mito
                        Mitochondrial carrier1074 coenzyme A transport                     M                     LEU5                    1
YHR004C     NEM1                              1341 sporulation
                        Protein of the nuclear envelope required unknown
                                                                    for the spherical shape of the nucleus; required for normal sporulation
                                                                                           M          Mito       NEM1                    1
YHR038W     RRF1        Mitochondrial ribosome recycling biosynthesis
                                               693 protein factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory f
                                                                    translation termination factor activity
                                                                                           M          Mito       RRF1                    0
YHR050W     SMF2        Divalent metal ion transporter involvedionmanganese inorganic cation transporter activity for di-valent and tri-valent metals; post-
                                              1650 manganese indi-, tri-valent homeostasis; has broadvacuole
                                                                      transport            M                     SMF2
                                                                                                      Mito, specificity                11
YHR067W     HTD2        Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in M acid biosynthesis, required for respiratory growth and for norm
                                               843 fatty acid biosynthesis
                                                                    3-hydroxyacyl-[acyl-carrier protein] dehydratase activity
                                                                                           fatty      Mito       HTD2                    0
YHR083W     SAM35       Essential component 990 mitochondral protein import
                                                of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membra
                                                                    protein binding        M          Mito       SAM35                   0
YHR091C     MSR1                              1932 protein biosynthesis
                        Mitochondrial arginyl-tRNA synthetase arginine-tRNA ligase M       activity   Mito       MSR1                    0
YHR120W     MSH1        DNA-binding protein2880 DNA repair
                                               of the mitochondria ATP binding
                                                                    involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatc
                                                                                           M          Mito       MSH1                    0
YHR168W     MTG2                              1500 protein Obg family;
                                                                    GTPase activity        M          Mito       MTG2
                        Putative GTPase, member of the biosynthesisperipheral protein of the mitochondrial inner membrane that0associates with the lar
YHR189W     PTH1        One of two (see also 573 protein biosynthesis
                                               PTH2) mitochondrially-localized peptidyl-tRNA activity
                                                                    aminoacyl-tRNA hydrolase hydrolases; dispensable for cell growth and for mitochondria
                                                                                           M          Mito       PTH1                    0
YIL042C     YIL042C                           1185 unknown
                        Mitochondrial kinase, phosphorylates pyruvate dehydrogenase alpha subunit Pda1p
                                                                    kinase activity        M          Mito       YIL042C                 0
YIL062C     ARC15                              465 mitochondrial organization the biogenesis
                                                                     required molecule activity
                                                                                           M          Mito       ARC15
                        Subunit of the ARP2/3 complex, which isstructural forand motility and integrity of cortical actin patches 0
YIL065C     FIS1        Mitochondrial outer membrane protein involved in membrane fission, required for localization of Dnm1p and Mdv1p during mitoch
                                               468 mitochondrial fission
                                                                    unknown                M          Mito       FIS1                    1
YIL077C     YIL077C                            963 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YIL077C purified mitochondria in high-through
                                                                      authentic,           M          Mito       in highly               2
YIL087C     YIL087C                            474 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YIL087C purified mitochondria in high-through
                                                                      authentic,           M          Mito       in highly               0
YIL111W     COX5B                              544 c oxidase, which is the terminal cytochrome cMito
                                                                    cytochrome-c oxidase activity
                                                                                           M            mitochondrial inner
                        Subunit Vb of cytochromemitochondrial electron transport, member of theto oxygen COX5B membrane 1                electron transport chain
YIL114C     POR2                               846 ion transport voltage-gated ion-selective channel activity
                                                                                           M          Mito       POR2                    0
                        Putative mitochondrial porin (voltage-dependent anion channel), related to Por1p but not required for mitochondrial membrane pe
YIL129C     TAO3        Protein involved in cell morphogenesis and proliferation, associated with Mito
                                              7131 cellular morphogenesis
                                                                    unknown                M                     TAO3                    0
                                                                                                      protein kinase Cbk1p; mutants activate OCH1 transcript
YIL134W     FLX1                               936 FAD flavin adenine dinucleotide (FAD),  M          Mito       FLX1                    3
                        Protein required for transport oftransport FAD transporter activity a synthesis product of riboflavin, across the mitochondrial mem
YIL136W     OM45                              1182 unknown
                        Protein of unknown function, major constituent of the mitochondrial outerMito
                                                                    unknown                M                     OM45                    1
                                                                                                        membrane; located on the outer (cytosolic) face of the
YIL155C     GUT2        Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non
                                              1950 carbohydrate metabolism
                                                                    glycerol-3-phosphate M            Mito
                                                                                           dehydrogenase activityGUT2                    0
YIR021W     MRS1        Protein required for the splicing of intron splicing
                                              1092 Group I two mitochondrial group I introns (BI3 Mito
                                                                    RNA binding            M                     MRS1                    0
                                                                                                      in COB and AI5beta in COX1); forms a splicing complex
YIR024C     YIR024C                            651 unknown
                        Protein of unknown function; the authentic, non-tagged proteinM detected in highly YIR024C
                                                                    unknown                 is        Mito                               in
                                                                                                                  purified mitochondria 1 high-throughput stud
YJL003W     COX16       Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase
                                               357 aerobic respiration
                                                                    unknown                M          Mito       COX16                   1
YJL023C     PET130                            1044 unknown
                        Protein required for respiratory growth; the authentic, non-tagged proteinMito
                                                                    unknown                M                     PET130                  0
                                                                                                       is detected in highly purified mitochondria in high-throug
YJL062W-A   YJL062W-A                          258 unknown
                        Putative protein of unknown function, identified based on comparison to related yeast species; the authentic, non-tagged protein
                                                                    unknown                M          Mito       YJL062W-A               1
YJL102W     MEF2        Mitochondrial elongation factor involved in translational elongation activity
                                             2460 translational elongation elongationM
                                                                    translation              factor     Mito       MEF2                   0
YJL112W     MDV1                              the mitochondrial genome maintenance M membrane, required for mitochondrial fission; interacts with Fis
                                                                     the mitochondrial outer
                        Peripheral protein of2145cytosolic face ofunknown                               Mito       MDV1                   0
YJL131C     YJL131C                          1071 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YJL131Cpurified mitochondria in high-through
                                                                     authentic,             M           Mito       in highly              0
YJL133C-A   YJL133C-A                          225 unknown           authentic,             M           unknown in highly purified mitochondria in high-through
                        Putative protein of unknown function; theunknown non-tagged protein is detected YJL133C-A                         0
YJL161W     FMP33                              543 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP33 purified mitochondria in high-through
                                                                     authentic,             M           Mito       in highly              0
YJL166W     QCR8                                cytochrome-c reductase
                                                                    ubiquinol-cytochrome-c a component of QCR8
                                                                                            M           Mito
                        Subunit 8 of ubiquinol285 aerobic respiration complex, which isreductase activitythe mitochondrial inner 1         membrane electron tra
YJL180C     ATP12       Conserved protein required for assemblyunfolded protein binding
                                               978 protein complex assembly beta subunits into MitoF1 sector of mitochondrial F1F0 ATP synthase; mut
                                                                     of alpha and           M           the        ATP12                  0
YJR003C     YJR003C                          1620 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YJR003C
                                                                     authentic,             M           Mito                              0
                                                                                                                   in highly purified mitochondria in high-through
YJR034W     PET191      Protein required for assembly of cytochrome c oxidase; the authentic, non-tagged protein is detected in highly purified mitochond
                                               327 cytochrome c oxidase complex assembly
                                                                    unknown                 M           Mito       PET191                 0
YJR039W     YJR039W                          3366 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YJR039W
                                                                     authentic,             M           Mito                              0
                                                                                                                   in highly purified mitochondria in high-through
YJR080C     FMP26                            1185 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP26 purified mitochondria in high-through
                                                                     authentic,             M           Mito       in highly              0
YJR095W     SFC1        Mitochondrial succinate-fumarate transporter, transports succinate intoactivity
                                               969 fumarate transport
                                                                    succinate:fumarate antiporter and fumarate out of the mitochondrion; required for ethan
                                                                                            M           Mito       SFC1                   2
YJR111C     YJR111C                            852 unknown
                        Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
                                                                    unknown                 M           Mito       YJR111C                0
YKL055C     OAR1        Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a Mito II mitochondrial fatty acid synthase along with Mct1
                                               837 aerobic respiration
                                                                    3-oxoacyl-[acyl-carrier protein] reductase activity
                                                                                            M            type      OAR1                   0
YKL070W     YKL070W                            510 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YKL070W
                                                                     authentic,             M           Mito                              0
                                                                                                                   in highly purified mitochondria in high-through
YKL106W     AAT1                             1356 aspartate biosynthesis transaminase activity Mito
                                                                    aspartate               M                      AAT1                   0
                        Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine bio
YKL132C     RMA1                             1293 unknown
                        Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; th
                                                                    tetrahydrofolylpolyglutamate synthase activity
                                                                                            M           Mito       RMA1                   0
YKL134C     OCT 1       Mitochondrial intermediate peptidase, cleaves N-terminal residues of a subset of proteins upon import, after their cleavage by mi
                                             2313 iron ion homeostasis
                                                                    metallopeptidase activity
                                                                                            M           Mito       OCT 1                  0
YKL141W     SDH3        Cytochrome b subunit of succinate dehydrogenasedehydrogenase (ubiquinone) activity
                                               597 tricarboxylic acid cycle (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to t
                                                                    succinate               M           Mito       SDH3                   3
YKL142W     MRP8        Putative mitochondrial ribosomal protein,structural constituent M ribosomal protein S2
                                               660 protein biosynthesissimilarity to E. coli ribosome
                                                                     has                    of          Mito       MRP8                   0
YKL187C     YKL187C                          2253 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YKL187C
                                                                     authentic,             M           Mito                              4
                                                                                                                   in highly purified mitochondria in high-through
YKL194C     MST1                             1389 threonyl-tRNA aminoacylation              M
                        Mitochondrial threonyl-tRNA synthetase threonine-tRNA ligase activity Mito                 MST1                   0
YKR049C     FMP46                              402 unknown
                        Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria i
                                                                    unknown                 M           Mito       FMP46                  0
YKR052C     MRS4                               915 RNA splicing iron ion transporter activity
                                                                                            M           Mito        to and                0
                        Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similarMRS4 functionally redundant with Mrs3p; fun
YLL006W     MMM1        Mitochondrial outer membrane protein required for normal mitochondrial Mito
                                             1281 mitochondrial organization and biogenesis
                                                                    unknown                 M                      MMM1                   1
                                                                                                        morphology and mtDNA stability; involved in tethering m
YLL027W     ISA1        Mitochondrial matrix protein involved in biogenesis of the iron-sulfur (Fe/S) cluster of Fe/S proteins, isa1 deletion causes loss of m
                                               753 iron ion transport
                                                                    unknown                 M           Mito       ISA1                   0
YLL060C     GTT2                               702 capable of metabolism transferase activity Mito
                                                                    glutathione             M                      GTT2
                        Glutathione S-transferase glutathione homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p 0
YLR067C     PET309                           2898 protein the COX1 mRNA,regulator activitystability of intron-containing COX1 primary transcripts; locate
                                                                    translation also influences
                        Specific translational activator for biosynthesis                   M           membranePET309                    0
YLR087C     CSF1                             8877 fermentation unknown                      M           Mito       CSF1                   1
                        Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria
YLR091W     YLR091W                            882 unknown
                        Protein of unknown function; the authentic, non-tagged proteinM detected in highly YLR091W
                                                                    unknown                   is        Mito                               in
                                                                                                                    purified mitochondria 0 high-throughput stud
YLR139C     SLS1                             1932 protein biosynthesis
                                                                    unknown                 M           membraneSLS1
                        Mitochondrial membrane protein required for assembly of respiratory-chain enzyme complexes III and IV;0coordinates expression
YLR142W     PUT1                             1431 glutamate biosynthesis
                                                                    proline protein involved in utilization
                                                                                            M                      PUT1                   0
                        Proline oxidase, nuclear-encoded mitochondrial dehydrogenase activity Mito of proline as sole nitrogen source; PUT1 transcriptio
YLR164W     YLR164W                            507 unknown
                        Protein of unknown function that localizes to the mitochondrialM
                                                                    unknown                             Mito       YLR164W
                                                                                             inner membrane; similar to Tim18p and to 0    Sdh4p
YLR188W     MDL1        Half-type ATP-binding cassette (ABC) transporteractivity inner M
                                             2088 oligopeptide transport of the
                                                                    ATPase                              Mito       MDL1                    of
                                                                                            mitochondrial membrane, mediates export 5 peptides generated u
YLR190W     MMR1                             1476 the mitochondrial outer membrane, M
                                                                    unknown                 localizes Mito         MMR1                   0
                        Phosphorylated protein of mitochondrial organization and biogenesis only to mitochondria of the bud; interacts with Myo2p to m
YLR201C     COQ9        Mitochondrial inner membrane protein required for ubiquinone M
                                               783 aerobic respiration
                                                                    unknown                             Mito       COQ9                   0
                                                                                             (coenzyme Q) biosynthesis, which in turn is required for respirator
YLR239C     LIP2        Lipoyl ligase, involved in the modificationligase activity
                                               987 protein-lipoylation mitochondrial enzymes by the attachment of lipoic acid groups
                                                                     of                     M           Mito       LIP2                   0
YLR251W     SYM1        Protein required for ethanol metabolism; unknownby heat shock and localized to the inner mitochondrial membrane; homologous
                                               594 ethanol metabolism
                                                                    induced                 M           Mito       SYM1                   0
YLR253W     YLR253W                          1710 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YLR253W
                                                                     authentic,             M           Mito                              0
                                                                                                                   in highly purified mitochondria in high-through
YLR368W     MDM30       F-box protein; physically associates withorganization and biogenesis forMito
                                             1797 mitochondrial unknown
                                                                    mitochondria and is required
                                                                                            M                      MDM30                  0
                                                                                                          normal mitochondrial fusion in rich medium, during spo
YLR382C     NAM2        Mitochondrial leucyl-tRNAGroup I intron splicinga direct role inM
                                             2685 synthetase, also has binding
                                                                    mRNA                                  several mitochondrial group I introns; indirectly required
                                                                                             splicing ofMito       NAM2                   0
YLR390W     ECM19                              339 cell wall organization and non-tagged
                                                                     authentic,             M           unknown in highly                 1
                        Putative protein of unknown function; theunknown biogenesis protein is detected ECM19 purified mitochondria in high-through
YLR393W     ATP10                              840 protein complex assemblybinding M
                                                                    chaperone                           Mito       ATP10
                        Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP 0            synthase, interacts gen
YLR395C     COX8                               237 aerobic respiration
                                                                    cytochrome-c oxidase activity Mito
                                                                                            M                      COX8
                        Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane1electron transport chai
YLR426W     YLR426W                          1052 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YLR426W
                                                                     authentic,             M           Mito                              3
                                                                                                                   in highly purified mitochondria in high-through
YML030W     YML030W                            480 unknown
                        Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YML030W is not a
                                                                    unknown                 M           Mito       YML030W                2
YML060W     OGG1                             1131 DNA that          purine-specific 7,8-dihydro-8-oxoguanine residues located opposite
                                                                                            M           Mito       OGG1
                        Mitochondrial glycosylase/lyaserepairspecifically excisesoxidized base lesion DNA N-glycosylase activity 0 cytosine or thymine
YML129C     COX14                              213 aerobic respiration
                                                                    unknown                 M           oxidase
                        Mitochondrial membrane protein, required for assembly of cytochrome c membraneCOX14                               1
YML133C     YML133C                          4224 unknown
                        Putative protein of unknown function withhelicase activity
                                                                      similarity to helicases; the authentic, non-tagged protein is detected in highly purified m
                                                                                            M           Mito       YML133C                0
YMR023C     MSS1        Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of th
                                             1581 protein biosynthesisbinding
                                                                    GTP                     M           Mito       MSS1                   0
YMR056C     AAC1        Mitochondrial inner membrane ADP/ATPATP:ADP antiporter activity
                                               930 aerobic respiration
                                                                     translocator, exchanges cytosolic ADP forAAC1
                                                                                            M           Mito                              4
                                                                                                                     mitochondrially synthesized ATP; Aac1p is a
YMR060C     SAM37       Component of the mitochondrialMM organizationbinding and assembly machinery SAM37complex; required for the sorting of so
                                               984 outer outer membraneand biogenesis
                                                                    protein sorting         M           Mito       (SAM)                  0
YMR097C     MTG1        Peripheral GTPase of the protein biosynthesismembrane, essential for respiratory competence, likely functions in assembly of th
                                             1104 mitochondrial inner
                                                                    GTPase activity         M           Mito       MTG1                   0
YMR150C     IMP1        Catalytic subunit of the mitochondrial inner membrane peptidase complex, requiredIMP1
                                               573 mitochondrial protein processing
                                                                    peptidase activity      M           Mito                              0
                                                                                                                    for maturation of mitochondrial proteins of th
YMR157C     FMP39                              768 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP39 purified mitochondria in high-through
                                                                     authentic,             M           Mito       in highly              0
YMR177W     MMT1        Putative metal transporteriron ion homeostasis
                                             1533 involved in mitochondrial iron accumulation; closely related to Mmt2p
                                                                    unknown                 M           Mito       MMT1                   1
YMR228W     MTF1        Mitochondrial RNA polymerase specificity factor with structural similarity Mito
                                             1026 transcription from mitochondrial promoter
                                                                    S-adenosylmethionine-dependent methyltransferase activity
                                                                                            M           to S-adenosylmethionine-dependent methyltransferases
                                                                                                                   MTF1                   0
YMR256C     COX7                               183 c oxidase, which is
                                                                    cytochrome-c oxidase activity Mito
                                                                                            M                      COX7
                        Subunit VII of cytochromeaerobic respiration the terminal member of the mitochondrial inner membrane 1             electron transport chain
YMR257C     PET111      Specific translational activator for biosynthesis
                                             2403 protein the COX2 mRNA,regulatorinM mitochondrial inner membrane
                                                                    translation located activity
                                                                                             the        Mito       PET111                 0
YMR282C     AEP2        Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA;AEP2
                                              1743 protein biosynthesis
                                                                    unknown                M           Mito                               0
                                                                                                                    exhibits genetic interaction with the OLI1 mR
YMR293C     YMR293C     Putative protein similar to aerobic respiration
                                              1395 bacterial glutamyl-tRNAactivity
                                                                    amidase amidotransferases; may be involved in aerobic respiration; the authentic, non-
                                                                                           M           Mito        YMR293C                0
YMR306W     FKS3                              5358 unknown
                        Protein of unknown function, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non
                                                                    1,3-beta-glucan synthase activity
                                                                                           M           Mito        FKS3                  18
YNL003C     PET8                               855 S-adenosylmethionine transport membrane, Mito
                                                                    mitochondrial inner M              member PET8                        2
                        S-adenosylmethionine transporter of the S-adenosylmethionine transporter activityof the mitochondrial carrier family; required for
YNL052W     COX5A                              462 c oxidase, which is
                                                                    cytochrome-c oxidase activity Mito
                                                                                           M                       COX5A
                        Subunit Va of cytochromeaerobic respiration the terminal member of the mitochondrial inner membrane 1              electron transport chain
YNL070W     TOM7        Component of the TOM (translocase of protein import
                                               183 mitochondral outer membrane) complex responsible for recognition and initial import steps for all mitoc
                                                                    protein transporter activity
                                                                                           M           Mito        TOM7                   0
YNL073W     MSK1        Mitochondrial lysine-tRNAlysyl-tRNA aminoacylation ligase activity
                                              1731 synthetase, required for import of both aminoacylated and deacylated forms of0
                                                                    lysine-tRNA            M           Mito        MSK1                    tRNA(Lys) into mitocho
YNL083W     SAL1                              1638 transport        transporter activity M             Mito        SAL1                   2
                        Probable transporter, member of the Ca2+-binding subfamily of the mitochondrial carrier family, with two EF-hand motifs; Pet9p
YNL100W     YNL100W                            705 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YNL100W
                                                                      authentic,           M           Mito                               0
                                                                                                                   in highly purified mitochondria in high-through
YNL144C     YNL144C                           2223 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YNL144C
                                                                      authentic,           M           Mito                               0
                                                                                                                   in highly purified mitochondria in high-through
YNL195C     YNL195C                            786 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YNL195C
                                                                      authentic,           M           Mito                               0
                                                                                                                   in highly purified mitochondria in high-through
YNL213C     YNL213C                            645 mitochondrial unknown
                                                                     lacks mitochondrial DNA and Mito
                                                                                           M                        on glycerol;          0
                        Protein of unknown function; null mutantorganization and biogenesis cannot grow YNL213C the authentic, non-tagged protein
YNL249C     MPA43                             1629 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected MPA43 purified mitochondria in high-through
                                                                      authentic,           M           Mito        in highly              0
YNR018W     YNR018W                            675 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YNR018W
                                                                      authentic,           M           Mito                               2
                                                                                                                   in highly purified mitochondria in high-through
YNR041C     COQ2        Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
                                              1119 ubiquinone metabolism
                                                                    prenyltransferase activity
                                                                                           M           Mito        COQ2                   5
YNR045W     PET494      Specific translational activator for biosynthesis
                                              1470 protein the COX3 mRNA that acts together with Pet54p and Pet122p; located in the mitochondrial inne
                                                                    translation regulator activity
                                                                                           M           Mito        PET494                 1
YNR070W     YNR070W     Putative transporter 4002 transport
                                              of the ATP-binding cassette (ABC) family,M to transmembrane movement of substances
                                                                    ATPase activity, coupled           Mito        YNR070W                9
                                                                                             implicated in pleiotropic drug resistance; the authentic, non-tagge
YOL008W     YOL008W                            protein, required for function of coenzyme Q in respiration by functioning in the delivery of Q<sub>6</sub>
                        Coenzyme Q binding 624 unknown              unknown                M           unknown YOL008W                    0
YOL009C     MDM12       Mitochondrial outer membrane protein, required for transmission of mitochondria toMDM12 cells; exists 0 a complex with Mmm
                                               816 mitochondrial organization and biogenesis
                                                                    unknown                M           Mito         daughter               in
YOL023W     IFM1        Mitochondrial translation initiation factor 2
                                              2031 translational initiation
                                                                    RNA binding            M           Mito        IFM1                   0
YOL025W     LAG2        Protein involved in determination of longevity; LAG2 gene is preferentially expressed in young cells; overexpression extends the
                                              1983 replicative cell unknown
                                                                    aging                  M           Mito        LAG2                   0
YOL033W     MSE1                              1611 protein biosynthesis
                                                                      encoded by a nuclear activity Mito
                        Mitochondrial glutamyl-tRNA synthetase,glutamate-tRNA ligase gene  M                       MSE1                   0
YOL077W-A   ATP19       Subunit k of the mitochondrial F1F0 ATPhydrogen-transportinglarge enzyme complex required for ATP synthesis; associated only
                                               207 ATP synthesis coupled proton transport synthase activity, rotational mechanism
                                                                      synthase, which is a M  ATP      Mito        ATP19                  0
YOL095C     HMI1                              2121 mitochondrial ATP-dependent DNA helicase, activity for the maintenance of the mitochondrial genom
                        Mitochondrial inner membrane localized genome maintenance M                    Mito
                                                                                            helicase required HMI1                        0
YOL096C     COQ3        O-methyltransferase, 939 ubiquinone metabolism
                                               catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mito
                                                                    hexaprenyldihydroxybenzoate methyltransferase activity
                                                                                           M           Mito        COQ3                   0
YOR011W     AUS1                              4185 sterol transportfamily, involved coupled of sterols and anaerobic growth
                                                                                           M           Mito        AUS1
                        Transporter of the ATP-binding cassette ATPase activity,in uptaketo transmembrane movement of substances         13
YOR017W     PET127      Protein with a role in2403 RNA processing
                                               mitochondrial RNA stability and/or processing, located in the mitochondrial membrane
                                                                    unknown                M           Mito        PET127                 0
YOR020W-A   YOR020W-A                          273 unknown          unknown                M           unknown YOR020W-A                  1
                        Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mi
YOR037W     CYC2        Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exh
                                              1101 Mitochondrial protein import
                                                                    unknown                M           Mito        CYC2                   0
YOR040W     GLO4                               858 carbohydrate metabolism of S-D-lactoylglutathione into GLO4
                                                                      hydrolysis           M           Mito
                        Mitochondrial glyoxalase II, catalyzes thehydroxyacylglutathione hydrolase activity glutathione and D-lactate     0
YOR045W     TOM6        Component of the TOM (translocase of protein import
                                               186 mitochondral outer membrane) complex responsible for recognition and initial import steps for all mitoc
                                                                    protein transporter activity
                                                                                           M           Mito        TOM6                   1
YOR100C     CRC1                               984 fatty carnitine transporter, required forantiporter activity CRC1
                        Mitochondrial inner membrane acid metabolism                       M           Mito                               3
                                                                    carnitine:acyl carnitine carnitine-dependent transport of acetyl-CoA from peroxisomes t
YOR205C     FMP38                             1671 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP38 purified mitochondria in high-through
                                                                      authentic,           M           Mito        in highly              0
YOR222W     ODC2                               924 mitochondrial transport
                                                                    intracellular transporter and 2-oxoglutarate from the mitochondrial matrix to the cytosol
                                                                                           M
                        Mitochondrial inner membrane transporter, exports 2-oxoadipateactivity Mito                ODC2                   0
YOR226C     ISU2        Conserved protein of 471 mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs
                                               the iron ion homeostasis
                                                                    unknown                M           Mito        ISU2                   0
YOR266W     PNT1        Mitochondrial inner membrane protein involved inand biogenesis
                                              1272 inner MM organization export of proteins fromMito mitochondrial matrix; overexpression of PNT1 confe
                                                                    unknown                M            the        PNT1                   1
YOR297C     TIM18       Component of the mitochondrial Tim54p-Tim22ptransporter activity in insertion of polytopic proteins into the inner membrane; ma
                                               579 protein targeting to membrane involved
                                                                    protein complex        M           Mito        TIM18                  0
YOR334W     MRS2        Mitochondrial inner membrane Mg(2+) channel, requiredtransport activity intramitochondrial Mg(2+) concentrations at the corr
                                              1413 mitochondrial magnesium ion transporter
                                                                    magnesium ion for maintenance of
                                                                                           M           Mito        MRS2                   0
YOR384W     FRE5                              2085 unknown            Fre2p; expression induced by Mito
                                                                                           M                       FRE5                   6
                        Putative ferric reductase with similarity toferric-chelate reductase activitylow iron levels; the authentic, non-tagged protein is detec
YPL029W     SUV3                              2214 RNA component
                        ATP-dependent RNA helicase, catabolism of the mitochondrial degradosome along with the RNase Dss1p; the degradosome as
                                                                    ATP-dependent RNA M                Mito
                                                                                            helicase activity      SUV3                   0
YPL040C     ISM1                              3009 protein biosynthesismutant is deficient in respiratory
                                                                      null                 M
                        Mitochondrial isoleucyl-tRNA synthetase,isoleucine-tRNA ligase activity Mito growth        ISM1                   0
YPL097W     MSY1                              1479 tyrosyl-tRNA tyrosine-tRNA              M
                        Mitochondrial tyrosyl-tRNA synthetase aminoacylation ligase activity           Mito        MSY1                   0
YPL103C     FMP30                             1407 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP30 purified mitochondria in high-through
                                                                      authentic,           M           Mito        in highly              1
YPL109C     YPL109C                           2126 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected YPL109C
                                                                      authentic,           M           Mito                               0
                                                                                                                   in highly purified mitochondria in high-through
YPL134C     ODC1                               933 mitochondrial transport
                                                                    intracellular transporter and 2-oxoglutarate from the mitochondrial matrix to the cytosol
                                                                                           M
                        Mitochondrial inner membrane transporter, exports 2-oxoadipateactivity Mito                ODC1                   1
YPL148C     PPT2                               522 protein-cofactor linkage activates mitochondrial acyl
                                                                    (PPTase),              M            activity PPT2                     0
                        Phosphopantetheine:protein transferase phosphopantetheinyltransferaseMito carrier protein (Acp1p) by phosphopantetheinyla
YPL167C     REV3                              4515 DNA repair       zeta DNA involved inM     activity zeta DNA Pol complex
                                                                                                                   REV3                   0
                        Catalytic subunit of DNA polymerase zeta, which ispolymerase DNA repair and translesion synthesis; required for mutagenesis in
YPL172C     COX10                             1389 protein complex first step in farnesyltransferase activity COX10heme A prosthetic group required for c
                                                                    protoheme              M
                        Heme A:farnesyltransferase, catalyzes theassemblyIX the conversion of Mito     protoheme to the                   4
YPL215W     CBP3        Mitochondrial protein required for complex assembly
                                              1008 protein assembly of ubiquinol cytochrome-c reductase complex (cytochrome bc1 complex); interacts w
                                                                    unknown                M           Mito        CBP3                   0
YPL222W     FMP40                             2067 unknown
                        Putative protein of unknown function; theunknown non-tagged protein is detected FMP40 purified mitochondria in high-through
                                                                      authentic,           M           Mito        in highly              0
YPL224C     MMT2        Putative metal transporteriron ion homeostasis
                                              1350 involved in mitochondrial iron accumulation; closely related to Mmt1p
                                                                    unknown                M           Mito        MMT2                   0
YPL271W     ATP15       Epsilon subunit of the189 sector of mitochondrial F1F0 ATP synthase, which isactivity, rotational mechanism
                                                F1 ATP synthesis hydrogen-transporting ATP synthase a large, evolutionarily conserved enzyme complex
                                                                     coupled proton transport
                                                                                           M           Mito        ATP15                  0
YPR006C     ICL2                              1728 the mitochondrial matrix,
                                                                    methylisocitrate lyaseM  activity Mito         ICL2                   0
                        2-methylisocitrate lyase ofpropionate metabolism functions in the methylcitrate cycle to catalyze the conversion of 2-methyliso
YPR011C     YPR011C                            981 transport        transporter activity M             Mito        YPR011C                0
                        Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified m
YPR020W     ATP20       Subunit g of the mitochondrial F1F0 ATPstructuralproton transport enzyme complex required for ATP synthesis; associated onl
                                               348 ATP synthesis coupled molecule activity
                                                                      synthase, which is a Mlarge      Mito        ATP20                  0
YPR021C     AGC1                              2709 acts both as a L-glutamate transporter activityaspartate-glutamate exchanger; involved in nitrogen met
                                                                     glutamate uniporter and as an Mito
                        Mitochondrial transporter, L-aspartate transport                   M                       AGC1                   0
YPR047W     MSF1        Mitochondrial phenylalanyl-tRNA synthetase alpha subunit, active as a monomer, unlike the cytoplasmic subunit which is active a
                                              1410 protein biosynthesis
                                                                    phenylalanine-tRNA ligase activity
                                                                                           M           Mito        MSF1                   0
YPR054W     SMK1                              1167 protein amino acid protein kinase (MAPK) required for production of the outer spore wall layers; negat
                                                                    MAP kinase activity
                        Middle sporulation-specific mitogen-activatedphosphorylation M                 Mito        SMK1                   0
YPR097W     YPR097W                           3222 unknown
                        Protein that contains a Phox homology (PX) domain and bindsM
                                                                    unknown                            Mito        YPR097W                0
                                                                                             phosphoinositides; the authentic, non-tagged protein is detected i
YPR098C     YPR098C                          582 unknown
                      Protein of unknown function, localized to unknown
                                                                   the mitochondrial outer membrane
                                                                                         M           Mito        YPR098C               3
YPR134W     MSS18     Nuclear encoded protein needed Ifor efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5
                                             807 Group intron splicing
                                                                  unknown                M           Mito        MSS18                 0
YPR140W     TAZ1      Lyso-phosphatidylcholine acyltransferase, required for normal M
                                            1146 phospholipid biosynthesis
                                                                  acyltransferase activity
                                                                                         phospholipid contentTAZ1
                                                                                                     Mito                              0
                                                                                                                  of mitochondrial membranes; may remodel a
YPR155C     NCA2      Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) andNCA2
                                            1851 aerobic respiration
                                                                  unknown                M           Mito                              1
                                                                                                                  8 (Atp8p) of the Fo-F1 ATP synthase; functio
YAL054C     ACS1                            2142 acetyl-CoA along with Acs2p, is theM, C
                                                                  acetate-CoA ligase activity source of acetyl-coA for histone acetlyation; expressed du
                      Acetyl-coA synthetase isoform which, biosynthesis                   nuclear Mito           ACS1                  0
YBR001C     NTH2                            2343 required to thermotolerance and may C
                                                                  alpha,alpha-trehalaseM, mediate resistance to other cellular stresses
                      Putative neutral trehalase,response forstress                        activity cytoplasm NTH2                     0
YBR238C     YBR238C                         2196 unknown          unknown                M, C        cytoplasm YBR238C                 0
                      Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and mitochondrio
YCR032W     BPH1      PProtein homologous to human Chediak-Higashi syndrome and murine Beige proteins, which are implicated in disease syndrom
                                            6504 cell wall organization and biogenesis C
                                                                  unknown                M,          Mito        BPH1                  0
YDL168W     SFA1                             containing both alcohol dehydrogenase andactivity cytoplasm SFA1
                                                                  alcohol dehydrogenase C
                      Bifunctional enzyme1161 formaldehyde catabolism                                                                  0
                                                                                         M, glutathione-dependent formaldehyde dehydrogenase activities,
YDR049W     YDR049W                         1899 unknown          factor that            M, C        cytoplasm YDR049W                 0
                      Zinc finger protein; putative transcription unknown may interact with proteins involved in histone acetylation or deacetylation; ma
YDR487C     RIB3      3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate
                                             627 aerobic respiration
                                                                  3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                                                                                         M, C        cytosol     RIB3                  0
YER033C     ZRG8                            3231 unknown          unknown                M, C         highly purified mitochondria in high-throughput studies;
                      Protein of unknown function; authentic, non-tagged protein is detected incytoplasm ZRG8                          0
YFR024C-A   LSB3                            C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein invo
                                                                  unknown
                      Protein containing a1474 actin filament organization               M, C        cytoplasm LSB3                    0
YGL125W     MET13                           1803 methionine reductase, constituent M, ribosome 5,10-methylenetetrahydrofolate to 5-methyltetrahydro
                                                                  structural             of C         of
                      Isozyme of methylenetetrahydrofolatemetabolism catalyzes the reductioncytoplasm MET13                            0
YGL197W     MDS3                            4464 kelch-like domain, of sporulation M, C
                                                                  unknown                            of early meiotic gene expression; required, with Pmd1p,
                      Protein with an N-terminalnegative regulation putative negative regulator cytoplasm MDS3                         0
YGL219C     MDM34                           1380 mitochondrial organization and biogenesis
                                                                  unknown                M, C         morphology and inheritance; localizes to dots on the mi
                      Mitochondrial outer membrane protein, required for normal mitochondrialcytoplasm MDM34                           0
YGL229C     SAP4                            2457 G1/S transition of mitotic cell cycle member of a family of similar proteins that 0
                                                                           serine/threonine C        cytoplasm SAP4
                      Protein required for function of the Sit4p protein phosphatase, M, phosphatase activity                          form complexes with Si
YGR147C     NAT2                             867 N-terminal acetyl group from acetyl coenzyme cytoplasm NAT2
                                                                  peptide alpha-N-acetyltransferase to the N-terminal methionine residues of proteins
                                                                                         M, C
                      N alpha-acetyl-transferase, transfers peptidyl-methionine acetylation           A activity                       1
YHR171W     ATG7                            1893 and dual specificity member of the E1 C
                                                                  ubiquitin-like         M, enzyme activity ATG7
                                                                                                     cytoplasm                         0
                      Autophagy-related proteinprotein targeting to vacuole conjugatingfamily of ubiquitin-activating enzymes; mediates the conjugation
YIL066C     RNR3                            2610 DNA replicationibonucleoside-diphosphate reductase activity
                                                                  r                      M, C        cytoplasm RNR3
                      Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting0           step in dNTP synthesis
YIL162W     SUC2                            1599 sucrose catabolism
                                                                  beta-fructofuranosidase C
                                                                                         M, form is regulated SUC2                     0
                      Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated activity cytoplasm by glucose repression, and an intracellular, no
YJL070C     YJL070C                         2667 unknown            similarity           M, C        the authentic, non-tagged protein is detected in highly p
                      Putative protein of unknown function withunknown to AMP deaminases; cytoplasm YJL070C                            0
YJR010W     MET3                            1536 methionine metabolism
                                                                  sulfate adenylyltransferase
                                                                                         M, activation, essential for
                                                                                                     cytoplasm                         0
                      ATP sulfurylase, catalyzes the primary step of intracellular sulfateC (ATP) activity MET3 assimilatory reduction of sulfate to sulf
YKL033W     YKL033W                         3117 unknown            authentic,           M, C        cytoplasm in highly purified mitochondria in high-through
                      Putative protein of unknown function; theunknown non-tagged protein is detected YKL033W                          0
YKR023W     YKR023W                         1593 unknown            authentic,           M, C        cytoplasm in highly purified mitochondria in high-through
                      Putative protein of unknown function; theunknown non-tagged protein is detected YKR023W                          0
YKR071C     DRE2                            1047 unknown          unknown
                      Protein of unknown function; mutation displays synthetic lethalM, C            cytoplasm DRE2
                                                                                           interaction with the pol3-13 allele of CDC20
YLL009C     COX17                            210 that transfers copper complex and Cox11p cytosol
                                                                  thioredoxin peroxidase activity
                                                                                         M, C                    COX17
                      Copper metallochaperonecytochrome c oxidase to Sco1p assembly for eventual delivery to cytochrome c oxidase      0
YLL013C     PUF3                            2640 mRNA catabolism, deadenylylation-dependentcytoplasm PUF3
                                                                  mRNA binding
                      Protein that regulates degradation of specific mRNAs such as M, C               decay                            0
                                                                                         COX17, binds almost exclusively to cytoplasmic mRNAs encoding
YMR038C     CCS1      Copper chaperone for superoxide dismutase Sod1p,dismutase copper chaperone activity Met-X-Cys-X2-Cys motif within the N
                                             750 intracellular copper ion transport
                                                                  superoxide involved in oxidative stress protection;
                                                                                         M, C        cytosol     CCS1                  0
YMR205C     PFK2                            2880 glycolysis       6-phosphofructokinase C  in glycolysis, indispensable                0
                      Beta subunit of heterooctameric phosphofructokinase involvedM,activity cytoplasm PFK2 for anaerobic growth, activated by fruc
YNL200C     YNL200C                          741 unknown            authentic,           M, C        cytoplasm in highly purified mitochondria in high-through
                      Putative protein of unknown function; theunknown non-tagged protein is detected YNL200C                          0
YOL038W     PRE6                             765 ubiquitin-dependent protein catabolism
                                                                  endopeptidase activity(GFP)-fusion proteinPRE6
                      20S proteasome alpha-type subunit; green fluorescent proteinM, C                            relocates            0
                                                                                                     proteasome complexfrom the cytosol to the mitochondri
YOL081W     IRA2                            9240 that negatively regulates RAS
                                                                  Ras GTPase activator activity from the GTP-
                                                                                         M, C                                          0
                      GTPase-activating proteinRas protein signal transduction by converting itcytoplasm IRA2to the GDP-bound inactive form, required
YOL141W     PPM2                            2088 unknown          C-terminal protein carboxyl
                                                                                         M, biochemical activity activity
                                                                                                     cytoplasm PPM2                    0
                      Putative carboxyl methyl transferase, has similarity to Ppm1p but C methyltransferasenot yet demonstrated; the authentic, non-ta
YOR347C     PYK2      Pyruvate kinase that1521 pyruvate metabolismby phosphorylation; PYK2cytosol
                                             appears to be modulated
                                                                  pyruvate kinase activity C
                                                                                         M,                      PYK2                  0
                                                                                                      transcription is repressed by glucose, and Pyk2p may b
YPL005W     AEP3                            1821 mRNA metabolism  unknown
                      Peripheral mitochondrial inner membrane protein, located on the C              cytoplasm membrane; stabilizes 0 bicistronic AAP1-AT
                                                                                         M, matrix face of the AEP3                     the
YPL072W     UBP16                           1500 protein deubiquitination
                                                                    outer mitochondrial membrane, probably not important for general mitochondrial functio
                                                                                         M, C
                      Deubiquitinating enzyme anchored to theubiquitin-specific protease activity    cytoplasm UBP16                   1
YPR002W     PDH1                            1551 participates in respiration, induced by diauxic shift; homologous to E. coli PrpD,0may take part in the co
                                                                  unknown
                      Mitochondrial protein that propionate metabolism                   M, C        cytoplasm PDH1
YPR184W     GDB1                            4611 glycogen catabolism
                                                                  4-alpha-glucanotransferase
                                                                                         M, alpha-1,6-amyloglucosidase activities, required for glycogen de
                      Glycogen debranching enzyme containing glucanotranferase andC activity         cytoplasm GDB1                    0
YKL056C     RBF18                            504 unknown          unknown                M, C, R cytoplasm protein;                    0
                      Protein that associates with ribosomes; homolog of translationally controlled tumor RBF18 green fluorescent protein (GFP)-fusio
YNL227C     JJJ1                            1773 endocytosis unknown                     M, C, R a late step of ribosome biogenesis; associated with the
                      Co-chaperone that stimulates the ATPase activity of Ssa1p, required for cytoplasm JJJ1                           0
YDR069C     DOA4                            required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosom
                      Ubiquitin hydrolase, 2781 endocytosis endopeptidase activity , D   M                       DOA4                  0
                                                                                                     endosome, proteosome, membrane, Mito
YGL023C     PIB2      Protein binding phosphatidylinositol 3-phosphate, involved in binding
                                            1908 vesicle-mediated transport
                                                                  phosphatidylinositol telomere-proximal repression of gene expression; similar to Fab1 a
                                                                                         M, D        Mito        PIB2                  0
YBR029C     CDS1                            1374 phosphatidylglycerol biosynthesis M, an
                                                                  phosphatidate cytidylyltransferase activity CDS1
                                                                                                     Mito                              6
                      Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); E enzyme that catalyzes that conversion of CTP + phosphate
YBR004C     GPI18                           1302 GPI anchor biosynthesis
                                                                  mannosyltransferase M, E
                                                                                         activity ER
                      Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers GPI18                          in
                                                                                                                  the second mannose 8 glycosylphosphatidyl
YER019W     ISC1      Inositol phosphosphingolipid phospholipase C, hydrolyzes activity E
                                            1434 response to salt stress
                                                                  phospholipase C inositolphosphosphingolipids, activated by phosphatidylserine, cardioli
                                                                                         M,          Mito        ISC1                  2
YMR065W     KAR5      Protein required for nuclear membrane fusion during karyogamy, E fusion the membrane with a soluble portion in the endopla
                                            1515 karyogamy during conjugation with cellular
                                                                  unknown                            Mito
                                                                                         M, localizes to         KAR5                  0
YJR073C     OPI3                             621 phosphatidylcholine biosynthesis M, E
                                                                  phosphatidyl-N-methylethanolamine catalyzes the last two steps
                                                                                                     Mito        OPI3
                      Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase),N-methyltransferase activity0 in phosphatidylcholine
YGL139W     YGL139W                         2409 similar to       unknown                 to the
                      Putative FAD transporter, unknown Flc1p and Flc2p; localizedM, E ER Mito                   YGL139W              10
YPL206C     YPL206C                          966 metabolism ofglycerophosphodiester phosphodiesteraseYPL206C
                                                                    glycerophosphocholine E, phosphatidylcholine; contains glycerophosphodiester phosph
                      Protein that plays a role inglycerophospholipid catabolism         M, to C cytoplasm activity                    0
YPL137C     GIP3                            3831 unknown          unknown                M, E, R, Ccytoplasm GIP3                      0
                      Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may i
YGL005C     COG7      Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in pr
                                             840 intra-Golgi transport
                                                                  unknown                M, G        Mito        COG7                  0
YJL094C     KHA1                            2622 the authentic, potassium:hydrogen antiporter activity purified mitochondria in high-throughput studies
                                                                  non-tagged protein isM,
                      Putative K+/H+ antiporter;monovalent inorganic cation transport G              Mito
                                                                                          detected in highly KHA1                     12
YKR027W     FMP50                           2298 unknown
                      Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group ofG related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p)
                                                                  unknown                M, 4        Mito        FMP50                 0
YOR070C     GYP1                            1914 vesicle-mediated transport family members Mito
                                                                  Rab the Rab            M, G                    GYP1                  0
                      Cis-golgi GTPase-activating protein (GAP) forGTPase activator activity Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p
YPL202C     AFT2                            1251 transcription activates genes involved in intracellular iron use and required for iron homeostasis and re
                                                                  TF activity            M, N        promoter
                      Iron-regulated transcriptional activator; initiation from RNA polymerase II nucleus AFT2                         0
YKL114C     APN1                            1104 DNA repair       DNA-(apurinic or apyrimidinic site) lyase activitydamage by oxidation and alkylating agen
                      Major apurinic/apyrimidinic endonuclease, 3'-repair diesteraseM, N             nucleus APN1
                                                                                          involved in repair of DNA                    0
YKR036C     CAF4                            1980 regulation of transcription, CCR4-NOT complex, interacts CAF4
                                                                  protein the DNA-dependent
                      WD40 repeat-containing protein associated with binding             M, N        Mito         in a Ccr4p-dependent0manner with Ssn2p; al
YDL164C     CDC9                            the nucleus and mitochondria, an essential enzyme nucleus Okazaki fragments during DNA replication; als
                                                                  DNA
                      DNA ligase found in2268 DNA recombination ligase (ATP) activity    M, N        that joins CDC9                   0
YGL240W   DOC1                               753 mitotic sister chromatidregulator theM, N
                                                                 enzyme segregation anaphaseMito
                    Processivity factor required for the ubiquitination activity of activity                     complex (APC), mediates the activity of the A
                                                                                                     promotingDOC1                   0
YMR232W   FUS2      Cytoplasmic protein 2034 regulation shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
                                           localized to the of termination of mating projection growth
                                                                 unknown                M, N        Mito       FUS2                  0
YDL003W   MCD1                            1701 for sister chromatid cohesion in mitosis and meiosis; subunit of the cohesin complex; expression is cel
                                                                 unknown
                    Essential protein required mitotic sister chromatid cohesion M, N               nucleus MCD1                     0
YNL292W   PUS4                            1212 tRNA modification pseudouridylate synthase activity
                                                                                        M, N        nucleus a highly                 0
                    Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), PUS4 conserved tRNA modification, in mito
YMR061W   RNA14                           2034 mRNA polyadenylylation
                                                                 RNA binding            M, in       nucleus RNA14                    0
                    Cleavage and polyadenylation factor I (CF I) component involved N cleavage and polyadenylation of mRNA 3' ends; bridges inte
YLR105C   SEN2                              splicing endonuclease, which is endonuclease activity
                                                                                        M, N        Mito
                    Subunit of the tRNA1134 tRNA splicing tRNA-intron composed of Sen2p, Sen15p, SEN2           Sen34p, and Sen54p;0Sen2p contains the ac
YAR008W   SEN34                              828 tRNA splicing tRNA-intron endonuclease activity
                                                                                        M, N        Mito        Sen34p,
                    Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, SEN34 and Sen54p;0Sen34p contains the a
YPL083C   SEN54                             splicing endonuclease, which is endonuclease activity
                                                                                        M, N        Mito        Sen34p,
                    Subunit of the tRNA1404 tRNA splicing tRNA-intron composed of Sen2p, Sen15p, SEN54 and Sen54p 0
YBL088C   TEL1                            8364 response to DNA length stimulus
                                                                 protein kinase activityM, N        nucleus TEL1
                    Protein kinase primarily involved in telomeredamageregulation; contributes to cell cycle checkpoint control0in response to DNA da
YDR120C   TRM1      tRNA methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosin
                                          1713 tRNA methylation (guanine-N2-)-methyltransferase activity
                                                                 tRNA                   M, N        Mito       TRM1                  0
YML021C   UNG1                            1080 DNA repair
                    Uracil-DNA glycosylase, required for repair of uracil in DNA formedactivity
                                                                 uracil DNA N-glycosylase by spontaneousUNG1
                                                                                        M, N        nucleus                          0
                                                                                                                 cytosine deamination, not required for strand
YPL283C   YRF1-7                          5728 telomerase-independent telomere highly expressed in the mutants lacking the 0
                                                                 DNA helicase activityM, N
                    Helicase encoded by the Y' element of subtelomeric regions,maintenance          nucleus YRF1-7                   telomerase component
YNL077W   APJ1                             of the HSP40 (DNAJ) family; overexpression N, C cytoplasm APJ1
                    Putative chaperone 1587 unknown                                     M,
                                                                 unfolded protein binding interferes with propagation of the [Psi+] 0prion; the authentic, no
YER168C   CCA1                            1641          nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are ge
                                                                 tRNA                   M, N, C cytoplasm CCA1
                    ATP (CTP):tRNA-specific tRNA modification adenylyltransferase activity                                           0
YGR098C   ESP1                            4893 mitotic activity cysteine-type endopeptidase activity
                                                                  (related segregation that C cytoplasm chromatid separation 0 mediating dissociatio
                    Separase with cysteine protease sister chromatidto caspases)M, N,promotes sister ESP1                            by
YAL062W   GDH3                              glutamate dehydrogenase, synthesizes glutamate from
                                                                 glutamate              M, N, C nucleus GDH3                         0
                    NADP(+)-dependent1374 glutamate biosynthesis dehydrogenase activity ammonia and alpha-ketoglutarate; rate of alpha-ket
YOR355W   GDS1                            1569 aerobic respiration growth on            M, carbon source; the authentic, non-tagged protein is detected in
                    Protein of unknown function, required forunknown glycerol as a N, C cytoplasm GDS1                               0
YDR305C   HNT2                               743 hydrolase; has similarity to the
                                                                 hydrolase              M, N, C cytoplasm HNT2                       0
                    Dinucleoside triphosphatenucleoside catabolism activitytumor suppressor FHIT and belongs to the histidine triad (HIT) superfa
YOR274W   MOD5                            1287 tRNA modification   isopentenyl transferase,N, C nucleus MOD5
                                                                                        M, required for                              0
                    Delta 2-isopentenyl pyrophosphate:tRNAtRNA isopentenyltransferase activity biosynthesis of the modified base isopentenyladen
YPL096W   PNG1                            1092 misfolded or incompletely synthesized protein catabolismPNG1
                                                                   for deglycosylation of misfolded glycoproteins during proteasome-dependent degradatio
                                                                                        M, N, C nucleus
                    Conserved peptide N-glycanase requiredpeptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity         0
YNR003C   RPC34                              954 transcription from RNA polymeraseis apromoter
                                                                 DNA-directed RNA III N, Cdeterminant in pol III
                                                                                        M, key      activity
                    RNA polymerase III subunit C34; interacts with TFIIIB70 andpolymerase cytoplasm RPC34 recruitment by0the preinitiation comple
YLR183C   TOS4                            1470 G1/S-specific transcription in mitoticM, N, C cytoplasm TOS4
                                                                 TF activity             cell cycle                                  0
                    Transcription factor that binds to a number of promoter regions, particularly promoters of some genes involved in pheromone res
YMR002W   YMR002W                            471 unknown         unknown                M, N, not an essential gene
                    Mitochondrial intermembrane space cysteine motif protein; MIC17 isC cytoplasm YMR002W                            0
YBR111C   YSA1                               696 unknown         phosphoribosyl-ATP diphosphatase
                                                                                        M, N, C cytoplasm YSA1
                    Nudix hydrolase family member with ADP-ribose pyrophosphatase activity activity                                  0
YML075C   HMG1                            3165 ergosterol biosynthesis
                                                                 hydroxymethylglutaryl-CoA E ER (NADPH) to mevalonate, which is a rate-limiting s
                                                                                        M, N, reductase
                    One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA activity HMG1                  7
YLR450W   HMG2      One of two isozymes of HMG-CoA reductase that convert HMG-CoAreductase (NADPH) activity step in sterol biosynthesis; ov
                                          3138 ergosterol biosynthesis
                                                                 hydroxymethylglutaryl-CoA Eto mevalonate, a rate-limiting
                                                                                        M, N,       ER         HMG2                  7
YPL186C   UIP4                               915 unknown         unknown                M, N, E ER             UIP4                  0
                    Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates
YOR004W   YOR004W                            765 rRNA processing unknown                M, subunit) processome involved in 40S ribosomal subunit biogen
                    Essential nucleolar protein that is a component of the SSU (smallN, R Mito                 YOR004W               0
YLR270W   DCS1                            1053 deadenylylation-dependent decapping on in a feedback DCS1
                                                                 hydrolase activity, function acid anhydrides, in phosphorus-containing anhydrides
                                                                                        M,                                           0
                    Non-essential hydrolase involved in mRNA decapping, mayactingN, R, Ccytoplasm mechanism to regulate deadenylation, contain
YLR424W   SPP382                          2127 nuclear mRNA Ntr2p; also forms a trimer, R, Ccytoplasm SPP382
                                                                   splicing, via        M, N,                                        0
                    Essential protein that forms a dimer with RNA binding spliceosome with Ntr2p and Prp43p, that is involved in spliceosome disas
YDR384C   ATO3                               828 transport       transporter activity M, P
                    Plasma membrane protein, regulation pattern suggests a possible role inMito                ATO3                  6
                                                                                                     export of ammonia from the cell; member of the TC 9.B
YEL063C   CAN1      Plasma membrane arginine permease,acid transport acid transporter activity
                                          1773 basic amino requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid
                                                                 basic amino            M, P        Mito       CAN1                 10
YNR002C   FUN34                              849 transport       transporter activity M, P          Mito       FUN34                 5
                    Putative transmembrane protein, involved in the export of ammonia, a starvation signal that promotes cell death in the center of a
YNR055C   HOL1                            1761 transport         transporter activity M, of         Mito       HOL1                 10
                    Putative ion transporter similar to the major facilitator superfamilyP transporters; mutations in membrane-spanning domains per
YFL011W   HXT10                           1641 hexose transport
                    Putative hexose transporter, expressed at low levels and expression is repressed by glucose
                                                                 glucose transporter activity
                                                                                        M, P        Mito       HXT10                11
YNL318C   HXT14                           1623 hexose transport  galactose transporter M, P
                                                                                         activity is induced HXT14glucose and repressed in high glucose
                    Protein with similarity to hexose transporter family members, expression Mito               in low              12
YKL217W   JEN1                             required for transport
                    Lactate transporter, 1851 lactateuptake of lactate and pyruvate; expression is derepressed by transcriptional activator Cat8p und
                                                                 lactate transporter activity
                                                                                        M, P        Mito       JEN1                 12
YGR260W   TNA1                            1605 plasma membrane permease, responsible permease low levels of nicotinic 12 expression of the g
                                                                 nicotinamide mononucleotide Mito
                                                                                        M,
                    High affinity nicotinic acid nicotinamide mononucleotide transport P for uptake ofactivity TNA1                 acid;
Q0140     VAR1      Mitochondrial ribosomal protein ofbiosynthesis
                                          1197 protein the small subunit, mitochondrially-encoded; polymorphic in different strains due to variation in
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       VAR1                  0
YBL090W   MRP21     Mitochondrial ribosomal protein ofbiosynthesis
                                             534 protein the large subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRP21                 0
YBR146W   MRPS9     Mitochondrial ribosomal protein ofbiosynthesis
                                             837 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPS9                 0
YBR251W   MRPS5     Mitochondrial ribosomal protein ofbiosynthesis
                                             924 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPS5                 0
YCR071C   IMG2      Mitochondrial ribosomal protein ofbiosynthesis
                                             441 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       IMG2                  0
YDL069C   CBS1      Mitochondrial translationalprotein biosynthesis mRNA; membrane proteinacid binding with translating ribosomes, acts on the C
                                             690 activator of the COB
                                                                 translation factor activity, nucleic that interacts
                                                                                        M, R        Mito       CBS1                  0
YDR337W   MRPS28    Mitochondrial ribosomal protein ofbiosynthesis
                                             861 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPS28                0
YDR462W   MRPL28    Mitochondrial ribosomal protein ofbiosynthesis
                                             444 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPL28                0
YDR494W   RSM28     Mitochondrial ribosomal protein ofbiosynthesis
                                          1086 protein the small subunit; genetic interactionsMito
                                                                 structural constituent M, ribosome
                                                                                         of R                   possible             0
                                                                                                     suggest a RSM28 role in promoting translation initiatio
YER050C   RSM18     Mitochondrial ribosomal protein ofbiosynthesis
                                             609 protein the small subunit, has similarity to E. coli S18 ribosomal protein
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       RSM18                 0
YGR215W   RSM27     Mitochondrial ribosomal protein ofbiosynthesis
                                             333 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       RSM27                 0
YGR287C   YGR287C                         1770 unknown
                    Protein of unknown function that may interact with ribosomes, M, R onO-glycosyl compounds
                                                                 hydrolase activity, hydrolyzing co-purification experiments; has similarity to alpha-D-glu
                                                                                         based      Mito       YGR287C               0
YHR080C   YHR080C                         4038 unknown
                    Protein of unknown function that may interact with ribosomes, M, R on co-purification experiments; the authentic, non-tagged pr
                                                                 unknown                 based      Mito       YHR080C               0
YKL003C   MRP17     Mitochondrial ribosomal protein ofbiosynthesis
                                             396 protein the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specifi
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRP17                 0
YML009C   MRPL39    Mitochondrial ribosomal protein ofbiosynthesis
                                             213 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPL39                0
YMR024W   MRPL3     Mitochondrial ribosomal protein ofbiosynthesis
                                          1173 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPL3                 0
YMR225C   MRPL44    Mitochondrial ribosomal protein ofbiosynthesis
                                             444 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPL44                0
YMR286W   MRPL33    Mitochondrial ribosomal protein ofbiosynthesis
                                             261 protein the large subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPL33                0
YNR036C   YNR036C                             may interact with ribosomes constituent M, ribosome experiments; similar to E. coli0and human mitochond
                                                                 structural based on co-purification
                    Mitochondrial protein;462 protein biosynthesis                       of R       Mito       YNR036C
YNR037C   RSM19     Mitochondrial ribosomal protein ofbiosynthesis
                                             276 protein the small subunit, has similarity to E. coli S19 ribosomal protein
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       RSM19                 0
YPL013C   MRPS16    Mitochondrial ribosomal protein ofbiosynthesis
                                             366 protein the small subunit
                                                                 structural constituent M, ribosome
                                                                                         of R       Mito       MRPS16                0
YPL132W     COX11                             903 aerobic respiration
                                                                   copper ion binding M, R            Mito       COX11                  c
                      Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome1 oxidase; association
YPR100W     MRPL51    Mitochondrial ribosomal protein ofbiosynthesis
                                              423 protein the large subunit
                                                                   structural constituent M, ribosome
                                                                                          of R        Mito       MRPL51                0
YPR166C     MRP2      Mitochondrial ribosomal protein ofbiosynthesis
                                              348 protein the small subunit
                                                                   structural constituent M, ribosome
                                                                                          of R        Mito       MRP2                  0
YMR080C     NAM7                            2916 mRNA the SFIATP-dependent RNA M, R, C activity
                      ATP-dependent RNA helicase of catabolism                             for nonsense mediated mRNA decay and 0 efficient translation t
                                                                    superfamily, required helicase cytoplasm NAM7                      for
YPL105C     YPL105C                         2550 unknown           unknown                based on cytoplasm YPL105C                   0
                      Protein of unknown function that may interact with ribosomes, M, R, C co-purification experiments; authentic, non-tagged prote
YLR390W-A   CCW14                             wall glycoprotein, present in the inner layer W the cell wall
                                                                   structural constituent M, of
                      Covalently linked cell 717 cell wall organization and biogenesisof cell wallMito           CCW14                 0
YNL066W     SUN4                            1263 glucanases, organization and biogenesis
                                                                   glucosidase activity M, W          cell wall SUN4
                      Cell wall protein related tomitochondrialpossibly involved in cell wall septation; member of the SUN family0
YKR042W     UTH1                            1353 mitochondrial organization SUN family W
                                                                   unknown
                      Mitochondrial outer membrane and cell wall localizedand biogenesis              cell wall for mitochondrial autophagy; involved in the o
                                                                                          M, member required UTH1                      0
YDR256C     CTA1      Catalase A, breaks down hydrogen peroxide inoxygen speciesM, X
                                            1548 oxygen and reactive the peroxisomalmetabolism
                                                                   catalase activity                  Mito       CTA1                  0
                                                                                             matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta
YBR236C     ABD1                            1311 mRNA capping of a methyl group from S-adenosylmethionine to
                                                                                          N           nucleus ABD1                     0
                      Methyltransferase, catalyzes the transfermRNA (guanine-N7-)-methyltransferase activity the GpppN terminus of capped mRNA
YKL112W     ABF1      DNA binding protein2196 possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA r
                                             with DNA replication NA binding
                                                                   D                      N                      ABF1
                                                                                                      nuclear chromatin                0
YPL267W     ACM1                              630 unknown          unknown                N           and may ACM1                     0
                      Cell cycle regulated protein of unknown function; associated with Cdh1p unknown supress the APC/C[Cdh1]-mediated proteolysi
YDR448W     ADA2                            1305 histone acetylation
                                                                   transcription SAGA transcriptional adaptor/HAT
                                                                                          N                      ADA2                  0
                      Transcription coactivator, component of the ADA and coactivator activity SAGA complex (histone acetyltransferase) complexes
YOR023C     AHC1                            1701 histone acetylation
                                                                   histone acetyltransferase structural integrity of the complex activator complex
                                                                                          N
                      Subunit of the Ada histone acetyltransferase complex, required for activity                AHC1
                                                                                                      Ada2/Gcn5/Ada3 transcription 0
YIL079C     AIR1                            that interacts with the arginine
                                                                   unknown                N           nucleus AIR1                     0
                      RING finger protein 1083 mRNA-nucleus export methyltransferase Hmt1p to regulate methylation of Npl3p, which modulates N
YDL175C     AIR2                            that interacts with the arginine
                                                                   unknown                N           nucleus AIR2
                      RING finger protein 1035 mRNA-nucleus export methyltransferase Hmt1p; may regulate methylation of0Npl3p, which modulate
YGL021W     ALK1                            2283 mitosis
                      Protein kinase; accumulation and phosphorylation are periodicN kinase activity
                                                                   protein serine/threonine           nucleus     phosphorylated in response to DNA damage
                                                                                           during the cell cycle;ALK1                  0
YBR211C     AME1      Essential kinetochore975 attachment of spindle microtubules to spindle pole bodies; component of the kinetochore sub-complex
                                               protein associated unknown
                                                                    with microtubules and kinetochore
                                                                                          N                      AME1
                                                                                                      spindle pole body                0
YNL172W     APC1                            5247 mitotic sister chromatid segregation N
                                                                   protein binding                    anaphase-promoting complex ligase required for degrad
                      Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which isAPC1           a ubiquitin-protein 0
YLR127C     APC2                            2562 mitotic sister chromatid segregation N
                                                                   protein binding                    anaphase-promoting ligase required for degradation of
                                                                                                                 APC2
                      Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-proteincomplex 0
YDR118W     APC4                            1959 mitotic sister chromatid segregation N
                                                                   protein binding                    anaphase-promoting ligase required for degradation of
                                                                                                                 APC4
                      Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-proteincomplex 0
YOR249C     APC5                            2058 mitotic sister chromatid segregation N
                                                                   protein binding                    anaphase-promoting ligase required for degradation of
                                                                                                                 APC5
                      Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-proteincomplex 0
YML099C     ARG81                           2643 arginine metabolism
                                                                   transcription cofactor N
                                                                                          activity nucleus ARG81                       0
                      Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive
YJL081C     ARP4                            1470 regulation of transcriptionbinding
                                                                   chromatin from RNANcomponent of chromatin-remodeling enzyme complexes
                                                                                                      nucleus ARP4
                      Nuclear actin-related protein involved in chromatin remodeling,polymerase II promoter                            0
YNL059C     ARP5                            2268 protein targeting to vacuole
                                                                   unknown                N           nucleus ARP5                     0
                      Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YPR034W     ARP7                             involved in transcriptional regulation; subunit II the chromatin remodeling Snf/Swi complex
                                                                   general                N
                      Actin-related protein1434 chromatin remodeling RNA polymerase of TF activity    nucleus ARP7                     0
YOR141C     ARP8                            2646 unknown           unknown                N           nucleus ARP8                     0
                      Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YMR033W     ARP9                             involved in transcriptional regulation; subunit II the chromatin remodeling Snf/Swi complex
                                                                   general                N
                      Actin-related protein1490 chromatin remodeling RNA polymerase of TF activity    nucleus ARP9                     0
YOR058C     ASE1                            2658 mitotic microtubule-associated protein
                                                                    organization and biogenesis in nucleus microtubule
                                                                                                      spindle    ASE1                  0
                      Mitotic spindle midzone localized spindle microtubule binding N (MAP) family member; required for spindle elongation and stabi
YJL115W     ASF1                              840 DNA damage response, signal transduction resulting in induction ofcomplex that
                                                                     chromatin assembly N   activity chromatin assembly protein
                                                                                                                 ASF1
                      Nucleosome assembly factor, involved intranscription regulatorand disassembly, anti-silencing apoptosis 0 causes derepression o
YIL130W     ASG1                            2895 unknown           unknown                N           nucleus ASG1
                      Proposed transcriptional activator, member of the Gal4p family of zinc cluster proteins                          0
YKL185W     ASH1                            1767 transcription; growth localized and repressor in the distal tip
                                                                   specific               N           nucleus                          0
                      Zinc-finger inhibitor of HO pseudohyphalmRNA istranscriptional translated activity ASH1 of anaphase cells, resulting in accumul
YNL159C     ASI2                              membrane protein unknown with Asi1p and
                                                                   that acts              N           membraneASI2                     2
                      Integral inner nuclear 870 ubiquitin-dependent protein catabolism Asi3p to ensure the fidelity of SPS-sensor signalling by maintai
YKL052C     ASK1                              879 mitotic spindle structural constituent couplesin nucleus ASK1 force produced
                                                                    organization and biogenesis kinetochores to the
                                                                                          of          nuclear
                      Essential subunit of the Dam1 complex (aka DASH complex), N cytoskeleton chromosome kinetochor 0by MT depolymerizatio
YDL088C     ASM4      Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p
                                            1587 mRNA-nucleus export molecule activity
                                                                   structural             N                      ASM4
                                                                                                      nuclear pore                     0
YOR113W     AZF1                            2745 regulation of in induction DNA-dependent
                                                                   DNA binding            N           nucleus AZF1
                      Zinc-finger transcription factor, involvedtranscription, of CLN3 transcription in response to glucose; genetic0and physical interactio
YKR099W     BAS1                            2436 transcription from RNA polymerase II promoterexpression of genes of the purine and histidine biosynth
                                                                   RNA polymerase II TF induced
                                                                                          N
                      Myb-related transcription factor involved in regulating basal and activity nucleus BAS1                          0
YLR399C     BDF1                            2061 DNA initiation transcription regulator activity nucleus BDF1the basal transcription factor TFIID; con
                      Protein involved in transcriptionrepair                             N
                                                                    at TATA-containing promoters; associates with                      0
YDL070W     BDF2                            1917 unknown            at TATA-containing promoters; nucleus BDF2the basal transcription factor TFIID; con
                      Protein involved in transcription initiation unknown                N           associates with                  0
YNL039W     BDP1                            RNA transcription initiation from factor TF activity TF TFIIIB complex
                                                                   RNA polymerase polymerase III promoter BDP1
                                                                                          N                                            0
                      Essential subunit of 1785 polymerase III transcription RNA III(TFIIIB), which is involved in transcription of genes encoding tRNAs,
YOR198C     BFR1                            1413 meiosis
                      Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy supp
                                                                   RNA binding            N                      BFR1
                                                                                                      nuclear envelope-ER              0
YCL029C     BIK1                            1323 mitotic spindle microtubule binding N
                                                                    organization and biogenesis in nucleus pole body
                                                                                                      spindle    BIK1                  0
                      Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid s
YFL007W     BLM3                            6432 protein catabolismproteasome activator N activity nucleus BLM3                        0
                      Proteosome activator subunit; found in association with core particles, with and without the 19S regulatory particle; required for re
YDL074C     BRE1                            2103 chromatin silencing
                                                                   ubiquitin-protein ligasehistone nucleus BRE1                        0
                      E3 ubiquitin ligase for Rad6p, required for the ubiquitination ofN activityH2B, recruitment of Rad6p to promoter chromatin and sub
YLR015W     BRE2                            1518 (Set1C) complex, which methylatesN
                      Subunit of the COMPASS chromatin silencing                            activity COMPASS4 and complex              0
                                                                   transcription regulator histone H3 on lysineBRE2 is required in transcriptional silencing n
YGR246C     BRF1                            1791 transcription initiation from polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP an
                                                                   RNA polymerase polymerase III promoter BRF1
                                                                                          N
                      TFIIIB B-related factor, one of three subunits of RNA RNA III TF activity TF TFIIIB complex                      0
YBL097W     BRN1                            2265 for chromosome condensation, likely
                                                                   unknown                            nucleus BRN1                     0
                      Essential protein required mitotic sister chromatid segregation N to function as an intrinsic component of the condensation machin
YGL174W     BUD13                             801 bud site selection
                                                                   unknown                N           nucleus splicing;                0
                      Subunit of the RES complex, which is required for nuclear pre-mRNA retention and BUD13 involved in bud-site selection; diploid
YLR074C     BUD20                             501 bud site selection
                                                                   unknown                N           nucleus BUD20                    0
                      Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
YMR014W     BUD22                           1560 bud site selectionunknown                N           nucleus BUD22                    0
                      Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
YLR226W     BUR2                             (Bur1p) protein kinase; Sgv1p and Bur2p comprise nucleus activity
                                                                   cyclin-dependent protein            regulator BUR2                  0
                      Cyclin for the Sgv1p1188 mitotic sister chromatid segregation N kinasea CDK-cyclin complex involved in transcriptional regulati
YER159C     BUR6      Subunit of a heterodimericnegative regulation regulatorcorepressor activity complex binds to TBP and can repress transcription
                                              429 NC2 transcription of transcription from RNA polymeraseBUR6
                                                                   transcription complex with Ncb2p;
                                                                                          N           nucleus     II promoter          0
YKL005C     BYE1                            1785 negative regulation contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperatu
                                                                   transcriptional elongation regulator activity II promoter
                                                                                          N           nucleus
                      Negative regulator of transcription elongation, of transcription from RNA polymeraseBYE1                         0
YML102W     CAC2                            1407 DNA repair        transcription Rlf2p and Msi1p) chromatin assembly synthesized
                                                                                          N                      CAC2
                      Component of the chromatin assembly complex (with regulator activity that assembles newlycomplex 0 histones onto recently
YNL288W     CAF40     Evolutionarily conserved subunit of the transcription complex involved in controlling mRNA initiation, elongation and degradation;
                                            1122 regulation of CCR4-NOT from RNAN
                                                                   unknown                            CCR4-NOT complex
                                                                                           polymerase II promoterCAF40                 0
YER048C     CAJ1                            1176 unknown           chaperone regulator N  contains leucine CAJ1                        0
                      Nuclear type II J heat shock protein of the E. coli dnaJ family,activity anucleus zipper-like motif, binds to non-native substrates
YPL048W     CAM1      Homolog of translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, m
                                            1248 regulation of translational elongationN
                                                                   translation                        cytosol
                                                                                           factor activity       CAM1                  0
YGR140W     CBF2                            2871 chromosome segregation multisubunit complex thatchromosomeCDEIII region of the centromere; C
                                                                   DNA bending
                      Essential kinetochore protein, component of the CBF3 activity N                            CBF2
                                                                                                      nuclear binds to the kinetochor 0
YDL220C     CDC13                           2775 telomere maintenance*
                                                                   single-stranded DNA binding regulates telomere replication through recruitment of spec
                      Single stranded DNA-binding protein found at TG1-3 telomereN        G-tails; nuclear CDC13        cap complex 0
YFR028C     CDC14   Protein phosphatase required for amino acid dephosphorylation
                                          1656 protein mitotic phosphoproteinthe nucleolus until liberatedCDC14 FEAR and Mitotic Exit Network in ana
                                                                exit; located in phosphatase activity
                                                                                        N          nucleus      by the                0
YKL022C     CDC16                         2523 mitotic sister chromatid segregation N
                                                                protein binding                    is a ubiquitin-protein complex 0
                                                                                                               CDC16
                    Subunit of the anaphase-promoting complex/cyclosome (APC/C), which anaphase-promoting ligase required for degradation of a
YDL102W     CDC2                          3294 nucleotide-excision repair for chromosomal DNA replication during mitosis and0
                                                                delta DNA               N
                    Catalytic subunit of DNA polymerase delta; requiredpolymerase activity replication CDC2    fork                    meiosis, intragenic rec
YOR074C     CDC21                           915 DNA-dependent DNA replication
                                                                thymidylate synthase N  activity nucleus CDC21                        0
                    Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YHR166C     CDC23                         1881 mitotic sister chromatid segregation N
                                                                protein binding                    is a ubiquitin-protein complex 0
                                                                                                               CDC23
                    Subunit of the anaphase-promoting complex/cyclosome (APC/C), which anaphase-promoting ligase required for degradation of a
YAL041W     CDC24                         exchange factor (GEF cell transducer factor)
                                                                signal polarity         N          nucleus CDC24                      0
                    Guanine nucleotide 2565 establishment of or GDP-releaseactivity for Cdc42p; required for polarity establishment and maintenan
YFR036W     CDC26                           375 mitotic sister chromatid segregation N
                                                                protein binding                    anaphase-promoting ligase required for degradation of
                                                                                                               CDC26
                    Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-proteincomplex 0
YBL084C     CDC27                         2277 mitotic sister chromatid segregation N
                                                                protein binding                    anaphase-promoting ligase required for degradation of
                                                                                                               CDC27
                    Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-proteincomplex 0
YOR257W     CDC31   Component of the spindle microtubule nucleation constituent N for SPB nuclear pore mitosis and meiosis II; homolog of mamm
                                            486 pole body (SPB) half-bridge, required cytoskeleton
                                                                structural              of                     CDC31
                                                                                                    duplication in                    0
YFL009W     CDC4                          2340
                    F-box protein required for G1/S transitionprotein bindingcycle N with Skp1p and Cdc53p to form a complex, SCFCdc4, which act
                                                        and G2/Mof mitotic cell
                                                                   transition, associates                      CDC4                   0
                                                                                                   nuclear SCF ubiquitin ligase complex
YLR103C     CDC45   DNA replication initiation factor;replicationto MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm1
                                          1953 DNA recruitedDNA binding
                                                                   initiation           N                      CDC45
                                                                                                   pre-replicative complex            0
YMR001C     CDC5                            similarity to amino acid phosphorylation Plo1p; found at bud neck, nucleus and SPBs; has multiple function
                                                                protein serine/threonine
                    Polo-like kinase with2118 protein Xenopus Plx1 and S. pombe N kinase activity  nucleus CDC5                       0
YGL190C     CDC55                         1581 protein biosynthesis phosphatase N
                                                                protein                  has multiple roles in CDC55 and protein biosynthesis; found in the
                    Non-essential regulatory subunit B of protein phosphatase 2A;type 2A activity  nucleus mitosis                    0
YJL194W     CDC6    Essential ATP-binding protein required for DNA replication, component of the pre-replicative complex (pre-RC) which requires OR
                                          1542 pre-replicative protein binding
                                                                complex formation and maintenance
                                                                                        N                      CDC6
                                                                                                   pre-replicative complex            0
YLR418C     CDC73   Constituent of Paf1 complex with RNA polymerase II,polymerase II promoter
                                          1182 RNA elongation from RNA Paf1p,transcriptionnucleusRtf1factor activity
                                                                RNA polymerase II Hpr1p, Ctr9,elongation and Ccr4p, distinct from Srb-containing Pol
                                                                                        N             Leo1,    CDC73                  0
YGL130W     CEG1                          1380 positive regulation of transcription from RNA polymerase CEG1
                                                                mRNA guanylyltransferase activity
                                                                                        N          nucleus II promoter                0
                    Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphoph
YMR168C     CEP3                          1827 mitotic spindle DNA bending complex
                                                                 checkpoint                        nuclear chromosome the centromere; contains an N-ter
                                                                                                               CEP3
                    Essential kinetochore protein, component of the CBF3 activity N that binds the CDEIII region ofkinetochor 0
YPL228W     CET1                          1650 mRNA capping
                    Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimerCET1other subunit is CEG1, a guanylyltransfe
                                                                polynucleotide 5'-phosphatase activity
                                                                                        N          nucleus      (the                  0
YLR115W     CFT2    Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A)
                                          2580 mRNA polyadenylylation
                                                                RNA binding             N                      CFT2
                                                                                                   mRNA polyadenylation factor complex0
YBR274W     CHK1                          1584 protein kinase,protein kinase activityN
                                                                  mediates mitotic cell            nucleus CHK1
                    DNA damage checkpoint effector amino acid phosphorylation cycle arrest via phosphorylation of Pds1p; 0             phosphorylated by che
YOR250C     CLP1                          factor I (CFI), involved in both
                                                                RNA binding             N          mRNA and polyadenylation steps
                                                                                                               CLP1
                    Subunit of cleavage 1338 mRNA polyadenylylation the endonucleolyitc cleavagecleavage factor complex 0 of mRNA 3'-end matu
YGR218W     CRM1                          3255 mRNA-nucleus export RNAs, and ribosomal subunits from the nucleus
                                                                protein
                    Major karyopherin, involved in export of proteins,carrier activityN            nucleus CRM1                       0
YHR146W     CRP1                           cruciform DNA        DNA
                    Protein that binds to1398 unknown structuresbinding                 N          nucleus CRP1                       0
YGL238W     CSE1                          2883 protein-nucleus export
                                                                importin-alpha export N receptor activity membrane
                                                                                                   nuclear CSE1                       0
                    Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required
YNR010W     CSE2                            450 transcription from RNA polymerase II promotermediator complex
                                                                RNA polymerase II transcription mediator activity
                                                                                        N                      CSE2                   0
                    Component of the Med9/10 module, which is a subcomplex within the RNA polymerase II Mediator complex; required for regulati
YKL049C     CSE4                            690 mitotic sister chromatid segregation N
                                                                centromeric for binding            kinetochore SE4
                                                                                                               C
                    Centromere protein that resembles histones, requiredDNAproper kinetochore function; homolog of human0              CENP-A
YNL232W     CSL4                            879 35S is an essential complex present
                                                                3'-5'-exoribonuclease activity nuclear exosome  cytoplasm that mediates
                                                                                                                                      0
                    Subunit of the exosome, whichprimary transcript processing Nin both nucleus andCSL4 (RNase complex) RNA processing an
YCR086W     CSM1                             forms a replication nknown
                                                                u
                    Nucleolar protein that573 DNAcomplex with Lrs4p which bindsN                     kinetochores during meiosis I to mediate accurate chro
                                                                                         Mam1p atnucleolus CSM1                       0
YMR048W     CSM3                            954 mitotic sister chromatid cohesion meiosis nucleus CSM3
                                                                unknown
                    Protein required for accurate chromosome segregation during N                                                     0
YJR084W     CSN12   Subunit of the Cop91272 adaptation to pheromone during conjugation orsignalosome complex
                                          signalosome, which unknown for deneddylation, with cellular CSN12
                                                                 is required            N                      fusion                 0
                                                                                                     removal of the ubiquitin-like protein Rub1p from Cdc53
YIL036W     CST6                           (bZIP) transcription factor from RNA polymerase II activates CST6
                                                                specific the polymerase II TF nucleus
                                                                                        N           promoter                          0
                    Basic leucine zipper1764 transcription initiationofRNAATF/CREB family, activity transcription of genes involved in utilization of
YMR094W     CTF13                          complex, which binds to the
                                                                DNA bending III element            nuclear chromosome DNA upon
                                                                                                               CTF13
                    Subunit of the CBF31437 kinetochore assembly CDE activity N of centromeres, bending thekinetochor 0binding, and may be in
YPL018W     CTF19                         1110 required for segregation
                                                                protein mitotic         N          nucleus CTF19                      0
                    Outer kinetochore protein,chromosomeaccurate bindingchromosome segregation; component of the kinetochore sub-complex CO
YLR381W     CTF3                          2202 that forms a segregation
                                                                protein binding         N          nuclear bind the kinetochore to 0
                                                                                                               CTF3
                    Outer kinetochore protein chromosomecomplex with Mcm16p and Mcm22p; maychromosome kinetochorspindle microtubules
YPL181W     CTI6                          transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to th
                                                                TF of transcription N
                    Protein that relieves 1521 positive regulationbinding                          nucleus CTI6                       0
YJL006C     CTK2                            972 domain kinase I (CTDK-I), which phosphorylates the
                                                                cyclin-dependent protein            regulator activity
                                                                                                               CTK2                   0
                    Beta subunit of C-terminalprotein amino acid phosphorylation N kinasenucleusC-terminal repeated domain of the RNA polymer
YML112W     CTK3                            891 protein amino acid I (CTDK-I), which kinasenucleus C-terminal repeated domain of the RNA polym
                                                                cyclin-dependent protein            activity
                    Gamma subunit of C-terminal domain kinase phosphorylation Nphosphorylates the CTK3                                0
YOL145C     CTR9                          3234 transcription from a polymerase II transcription and modulates activity
                                                                 is RNA polymerase II binds to elongation factor
                                                                                        N                                             0
                    Component of the Paf1p complex, whichRNAlarge complex thatpromoternucleus CTR9the activity of RNA polymerase II and is
YBR112C     CYC8                          2901 chromatin acts together coactivator activity as part CYC8
                                                                transcription           N                                             0
                    General transcriptional co-repressor,remodeling with Tup1p; also acts nucleus of a transcriptional co-activator complex that rec
YKR083C     DAD2                            402 mitotic spindle structural constituent couplesin nucleus DAD2 force produced
                                                                 organization and biogenesis kinetochores to the
                                                                                        of         nuclear
                    Essential subunit of the Dam1 complex (aka DASH complex), N cytoskeleton chromosome kinetochor 0by MT depolymerizatio
YBR233W-A   DAD3                            285 mitotic spindle protein binding biogenesis in nucleus DAD3 force produced by MT depolymerizatio
                                                                 organization and
                    Essential subunit of the Dam1 complex (aka DASH complex), N                    kinetochore
                                                                                        couples kinetochores to the                   0
YIR023W     DAL81                         genes in multiple transcription from RNAN II TF activity
                                                                specific RNA polymerase polymerase II pr