UNIVERSITY OF LIVERPOOL PHYSIOLOGY DEPARTMENT SAFETY CODE OF PRACTICE Updated August 2008 STATEMENT OF SAFETY POLICY. The Physiology department is committed to provide, so far as reasonably practicable, a safe and healthy working environment for its staff, students and visitors, and will adopt as a minimum standard the legal requirements for health and safety. Safety will be considered as a priority when individuals, or research groups are involved in the funding and development of research or teaching projects. Laboratories will produce and display a code of practice that will identify general and specific hazards in those areas. These codes of practice and the departmental safety policy will be reviewed regularly. A formal safety inspection will be carried out twice a year when health and safety practices and arrangements will be monitored. Accidents and incidents reported to the departmental safety co-ordinator or the laboratory superintendent‟s office will be recorded, a copy of which will be sent to the University safety advisors office. The reports will then be discussed at the next meeting of the departmental safety committee. The department will provide first aid materials at key points in the building, and further supplies may be obtained from the laboratory superintendents‟ office. Fire exits from the building will be clearly marked and appropriate extinguishers will be provided. A fire drill will take place sometime during the first term of each new academic year. Regular weekly testing of the fire alarm system will take place. A safety bulletin board will be provided where safety notices will be displayed and notification of departmental safety committee meetings will be posted. This information will also be available in each of the main laboratory areas. A suggestion box will be provided in the departmental common room to enable people to raise questions of safety anonymously if they so wish. Senior technical staff meet to discuss new safety circulars as required, and pass on relevant information. The departmental safety committee will meet as and when required or requested but no less than once a year. The committee will be responsible for departmental policy statements, implementation and development. The safety committee will review safety policy and performance and will prepare an action plan at the annual meeting to improve safety awareness and standards. DEPARTMENTAL SAFETY COMMITTEE. Professor. S. Dimaline. (Chair) Professor. O.H. Petersen. Dr. J. Coulson. (Radiation safety officer) Dr. J. Barclay Dr. O. Gerasimenko (Non ionising radiation safety) Mrs H. Davies. (Dept safety Co-ordinator) Mr S Bainbridge (Deputy Safety co-ordinator) Mr D Franks (Fire Safety Co-ordinator) Ms A Michael (Student representative) Mr G Williams This safety policy statement has been prepared and issued by the departmental safety committee. Safety Personnel. Safety co-ordinator. Mrs. H. Davies, Room A07 Roberts suite Ext. 44179 Deputy Safety co-ordinator .Mr. S Bainbridge, Workshop, Nuffield building. Ext.45336. Biological safety officer. Dr Jill Bubb White block. Ext.44770 Radiation safety officer. Dr. J. Coulson, Room 214 Nuffield Building. Ext.45850 Laser safety officer. Dr. O. Gerasimenko, Room 1.09 Blue block Ext.45322 Fire safety Co-ordinator Mr D Franks Room G.04a Yellow block Ext 45329 Senior technical staff. Mr. M. Houghton, Blue block, ext.45355 (First Aider) Mrs. H. Davies, Green block, Ext.44179 (First Aider) Mr. G. Williams, Red block, Ext.45793 (First Aider) Mr. W. Franks, Yellow block, Ext.45329 Mr. I. Kirk, White block, Ext.44770 (First Aider) Mrs. C. Levene, Teaching laboratory, Ext.45338 Mr. S. Bainbridge, Workshop, Ext.45336 School and Departmental responsibility. The head of the School of Biomedical Sciences is formally responsible to the senate and council of the University for ensuring, so far as is reasonable and practical, that the health and safety of staff, students and general public are not adversely affected by the activities of the School. Each individual working in the School has a legal obligation to take reasonable care for the health and safety of him or herself and of other persons, who may be affected by their acts or omissions. Within research groups, the academic members of staff leading these groups are responsible for the compliance of safety rules and regulations, and also the safe working practices of staff and students working in that group. Safety information. Safety notices are posted on the safety bulletin board in the common room A.05. Each main laboratory area has copies of safety circulars and memos. Each laboratory will also have a safety code and copies of specific assessments which must be consulted before you begin work in those areas. Copies of all university and departmental safety codes are kept by Mrs H Davies room A.07, Roberts Suite. These include: COSHH regulations. Guide lines for filling out risk assessments. GMAG regulations. Manual Handling policy. Display Screen Equipment. PUWER. Code of practice on Allergy to Laboratory Animals University safety policy for working with Radiation. University codes of practice are also available online via the University website. Work outside normal hours Staff working outside normal working hours must fill in details in the record book which may be found on the reception desk in the entrance foyer. Experimental work outside normal working hours must be arranged with a group head or supervisor. If you are working alone in the department outside normal working hours (8.00am – 6.00pm) you must inform security on Ext 43252 when you arrive and again when you leave the department. Unattended experiments. If you intend to carry on any experimental procedure or run any experimental equipment which will remain active and working after you have left the building you must complete a report form. A copy of the form must be displayed next to the ongoing experiment and another copy should be displayed close to the department entrance. Forms are available from the laboratory superintendent‟s office, room A.07,Roberts Suite. Accident and incident reporting. A report form should be completed for every personal injury or “near miss” caused as a result of the work you are doing or by University property. A copy of this report form will be sent to the University safety advisor. The form can be obtained from Mrs Helen Davies the safety co-ordinator, room A.07Roberts suite. Standard First Aid Procedures Eyes Irrigate thoroughly with cold running water for at least 15 minutes. Hold eyelids open with fingers to ensure adequate flushing. Seek medical attention. Skin Flush affected area with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes and wash before reuse. Seek medical attention. Mouth Wash out mouth with water. Do not induce vomiting. Seek medical attention. Inhalation Remove subject from exposure. Rest and keep warm. Give artificial respiration if patient is not breathing. NEVER GIVE ANYTHING BY MOUTH TO AN UNCONSCIOUS PATIENT. Seek medical attention if exposure has been excessive. Fire precautions and procedures. All members of staff and students working in the department should know: 1) The location and how to operate fire fighting equipment in the department. 2) The location and operation of fire alarms in the building, and how to call the fire brigade. 3) The location of alternative escape routes for leaving the building in the event of a fire. On discovering a fire. a) Sound the alarm. b) Telephone security (dial 2222); they will contact the fire brigade. c) Use the nearest appropriate fire extinguisher to put out or contain the fire, but do so without endangering yourself or others. If the fire is small and can be put out easily with an extinguisher, take immediate action to extinguish it. Report to security control (43252) so that a check can then be made that the fire is out. On hearing the alarm. Persons not engaged in containing or fighting the fire must: a) Make safe any experiment or equipment in use. b) Evacuate the building as quickly as possible, closing doors. c) Leave the building and go to assembly point. (DO NOT USE THE LIFT) The assembly point for the Crown street block is the car park outside the main entrance to the building. The assembly point for the Teaching Lab, Henry Wellcome wing, Nuffield third floor corridor and Workshop is outside the main entrance in the Ashton street car park. d) Never assume that the alarm is a false alarm. Act quickly but calmly. e) On no account must you re-enter the building until you are given clearance by a fire officer or the safety officer. Every member of staff or student will be issued with a copy of the Departmental fire regulations. They must be read and a receipt signed to confirm acceptance. Waste disposal. The department generates large amounts of waste, some of which will require special disposal methods. Waste bags are colour coded and it is essential that everyone observes the local rules concerning what may or may not be put into these bags. The members of the cleaning staff will only empty general waste bins, and they accept your assurance that these bins and bags do not contain any objects or materials which may be hazardous to their health. Waste other than general waste must be disposed of by laboratory staff as described below. Black bags. Normal non-hazardous waste .e.g. Paper, office waste. Yellow bags. Waste for incineration EXCLUDING sharps which should be disposed of separately. Bags must be sealed, labelled showing the name of the department, the users name and the type of waste in the bag. The bag should then be put into the yellow metal bin outside the rear entrance to the building and the bin should be locked. The waste will be collected once a week. Red, pink bags. Radioactive contaminated material. Bags must be sealed and put into the radioactive waste bin which will have been allocated to your laboratory. When the bin is full a waste disposal form should be filled in and taken to the laboratory superintendent‟s office. Arrangements will then be made to collect the waste. The waste bins are stored in the locked bunker at the rear of the Nuffield building. Sharps. Needles, Tips, blades etc. Put into special CINBIN containers. When the cinbins are 3/4 full the lid should be taped down to prevent it from opening and placed into the yellow metal waste bin outside the rear entrance to the building for incineration. Glass. Metal bins are provided in all laboratories marked GLASS ONLY. The bins should then be emptied into the general waste skips which are stored outside the rear of the building. Protective gloves and goggles are available in the laboratory and should be worn when removing glass bins to the general waste skips. Chemical waste. Check chemical data sheet and specific assessment for disposal method. If it is not acceptable to wash the waste down the sink or if there is any doubt about how to dispose of the material contact the senior technician in charge of your laboratory area. Waste paper. Waste paper bins are provided. These bins are for paper and general office waste only. Cardboard boxes should be folded flat and placed outside the rear of the building for collection. Do not leave empty boxes or boxes containing packing material for collection in the laboratory or office where they may cause an obstruction. Aqueous liquid radioactive waste. Aqueous liquid waste may be disposed of down a designated sink. The tap should be turned to give a gentle flow of water without splashing. The aqueous waste can then be slowly poured into the flow of water, taking care to avoid splashing which may contaminate the area surrounding the sink. The water should be allowed to continue to flow after the waste has been poured into the sink in order to flush the waste away. Date, isotope, and activity should be recorded in the log sheet. Aqueous biological waste. Aqueous biological waste for disposal down the sink must be autoclaved before disposal. The cooled waste may then be discarded down the sink taking care to flush the waste away with running water from the tap. General safety rules for the Physiology department. In any laboratory there are hazards and areas of risk to the laboratory worker. With some planning and forethought, the potential dangers can be minimised or removed completely. The following points are basic rules which should be observed in any laboratory. In addition, laboratories or working methods may have specific risks attached to them, and you should ask your supervisor to discuss these risks with you BEFORE you undertake any new work. STOP-THINK-IF IN DOUBT...ASK! 1. You should wear a suitable laboratory coat in the laboratory, not only to protect yourself from contamination by the materials you are working with, but also to protect others you come into contact with outside the laboratory. 2. Cuts, scratches, or abrasions on your hands or face should be covered before working in the laboratory even if you are working with non-hazardous, non- aggressive materials. 3. Eating, drinking, and the application of cosmetics in the laboratory is forbidden. 4. Use safe working practices at all times, and suitable protective equipment whenever necessary. 5. Read instructions and labels carefully and plan your experiment so that you may carry out your work safely. 6. Report any accidents, spillages, and potentially dangerous incidents to the technician or supervisor in charge of the laboratory. 7. Solutions and samples prepared during your experiment must be labelled to show details of these materials and any relevant hazard information. 8. At the end an experiment clean up your work area and dispose of any solutions and samples which are not needed. Any material which is to be stored for future use must be labelled correctly. 9. Before leaving the laboratory remove your lab coat and wash your hands. 10. Laboratory coats and gloves must not be worn in the staff common room, seminar room or meeting rooms. TEACHING LABORATORY CODE OF PRACTICE. 1. A suitable laboratory coat must be worn at all times in the teaching laboratory. 2. Eating, drinking and the application of makeup in the laboratory are forbidden. 3. Long hair must be tied back at all times. 4. Cuts and abrasions must be covered by a waterproof dressing. These may be obtained from the preparation room. 5. Contaminated paper waste must be put into the yellow waste bag provided. 6. Broken glass should be reported to the technician in charge of the laboratory. Sharps must be put into the CINBIN provided. 7. Gloves are available from the preparation room and must be worn when the practical involves contact with blood or urine. 8. All spillage‟s must be reported to the technician in charge of the laboratory. The area may need to be cleaned and disinfected. 9. All accidents must be reported to the technician in charge of the laboratory. An accident report form must be completed. 10. Before you leave the laboratory, please wash your hands. IF IN DOUBT .....ASK! SAFETY CODE OF PRACTICE DR.M.CLAGUE. 5th FLOOR NUFFIELD BUILDING Eating and drinking NOT allowed in laboratory areas. Laboratory coats must be worn in rooms when engaged in experiments. Laboratory coats must never be worn in room in offices or meeting rooms. They should be put on the hanger by the door and not left on chairs or stools. A specific protocol for working with any noxious substances must be worked out in advance of any experiments, by the experimenter together with Mike Clague. General safety matters arising can be discussed at weekly laboratory meetings. Department of Physiology Safety code of practice for GREEN BLOCK LABORATORIES supervised by Professor G. J. Dockray This code of practice should be read, and implemented by all members of staff, students and visiting workers to Green Block. Workers using the microbiology, tissue culture labs, handling human plasma, working with radiation or carrying out animal handling work should also be made aware of the relevant separate codes of practice. Revised August 2008 Green Block Safety Code Of Practice GENERAL 1. There is a safety information area located in room 1.22. 2. A risk assessment should be carried out as per University guidelines BEFORE any work is carried out. a COSHH assessment should be carried out for any hazardous chemical BEFORE it is ordered or used. Copies of all assessments and hazard data sheets are kept in the safety information area in room 1.22. 3. The department will issue individuals with a labcoat, these MUST be worn at all times. 4. Laboratory coats will be collected for laundry each week and a new coat will be issued. Spare lab coats are available ,contact Marie Johnson 5. The laboratory must be kept clean and tidy; spillages must be cleaned up immediately. 6. The last person leaving the laboratory must ensure all windows and doors are closed and locked. 7. Sinks for hand washing are readily available with soap and paper towels. They should not be used for any other purposes. 8. The FIRST AID BOX is sited between doors 1.32 and 1.33. All accidents should be reported to Helen Davies your first aider. CHEMICALS AND SOLVENTS 1. Toxic chemicals must be kept locked in the poisons cupboard in room 1.29 2. Risk assessments according to COSHH rules should be carried out before ordering or using the chemicals. Hazard data sheets for all chemicals should be kept. 3. There is a card index system cataloguing all chemicals and solvents, including their location and main hazards. This is located in the safety information area in room 1.22. 4. A list of chemicals used must be kept in lab books. 5. Chemicals should always be stored in their original containers. 6. Solvents should be kept in the appropriate solvent bins. 7. Waste solvents should be poured into the appropriate canisters in rooms 1.33 or 1.27. Halogenated or Non-Halogenated. 8. Old and unwanted chemicals should be labelled with waste tags and Helen Davies informed. The department of Chemistry will then collect or advise on disposal. RADIATION 1. Workers must register with the radiation protection office before starting work with radioactive compounds. 2. Radioactive compounds must be ordered and received in accordance with the local guidelines. 3. Radiation “badges” must be worn if issued along with laboratory coats and gloves. 4. Radiation work should only be carried out in designated laboratories or areas and all work should take place on a spill tray. 5. Any spillage of an isotope must be cleaned up immediately as per local rules. 6. Work areas and personnel should be monitored after work has been carried out and readings recorded in the contamination logbook for that area. 7. Solid waste should be disposed of in the appropriate bins (solid or scintillation vials) and the activity of the waste entered on the forms provided. 8. Aqueous waste must be disposed of down designated sinks. The activity disposed must be entered on the waste form provided. 9. In the event of a person becoming contaminated with radioactivity there is a safety shower in room 1.32 EQUIPMENT 1. No one should use a piece of equipment unless trained to do so. User lists are displayed near large pieces of equipment, only people on this list are authorised to use the equipment until full training is given. Risk assessments for the use of larger pieces of equipment have been carried out. 2. Any piece of equipment being used overnight should have a form with it clearly labelled with the users name, procedure and contact number. These forms are available from the safety information area in room 1.22. FRIDGE/FREEZER 1. All samples must be stored in appropriate containers clearly marked with: Owners name, Storage temperature, and Date. 2. Radioactive isotopes and samples should only be stored, clearly marked in locked fridges/freezers in rooms 1.22/1.33 3. Flammable solvents should only be stored in spark proof fridges/freezers. 4. Biohazards stored must be clearly marked and only stored in labelled fridges/freezers in accordance with the Human Materials regulations. BALANCES 1. Always clean and switch off after use. 2. Hazardous chemicals should be weighed out in the fume hood in room 1.32.Filter masks are available in this room. 3. After using the fine balance always enter details of the chemical used and its hazards. CENTRIFUGES 1. No one should use centrifuge unless trained to do so. 2. Ensure all buckets are balanced and loaded into the centrifuge correctly. 3. If there is a logbook, always fill it in at the end of each run. 4. Human plasma must only be spun in the centrifuge in room 1.32 If there is a spillage disinfect the buckets with Trigene solution. 5. Centrifuges should always be left clean and dry. Remove the buckets from the centrifuge at the end of each day. AUTOMATIC PIPETTES 1. Pipettes should always be stored upright. 2. Used tips should be put into the nearest sharps bin. These bins are then sealed, labelled and taken to the large yellow bins outside the back door. 3. Do not leave pipettes lying down with tips on. Anything in the tip may run back into the pipette. 4. Never try to use a pipette for a volume smaller or greater than its volume limit. ACCIDENTS 1. All accidents must be reported to a first aider Helen Davies. and an accident form must be filled in after an accident. 2. All cuts must be covered with waterproof plasters. 3. Do not leave anything lying about that may cause a trip hazard. 4. Wipe up ALL spills on floors and benches. Do not leave it for others to do. Work within specialist areas in Green Block There are additional codes of practice for working within specific areas within green block. These include: 1. Working in tissue culture labs 1.19b, 1.26, 1.35 2. Working in Microbiology lab 1.28 3. Working in RNA lab 1.36 4. Working in Molecular biology lab 1.33 5. Working with animals 6. Handling human samples. Safety Code of Practice for Blue Block The Safety Code of Practice for Blue Block details specific hazards and reiterates the general rules, should workers have turned to this section straight away. Important: for first-time workers in the Blue Block area No worker can commence laboratory work before the completion of the necessary safety data forms listed below: - 1. All work projects require that a risk assessment be carried out to assess the dangers relative to the project in question. Risk assessment forms can only be completed by the supervisor responsible for managing the worker/ project. A copy of the completed form must be stored safely by the worker/ applicant. 2. In addition to a project Risk Assessment form, many experimental procedures involve the use of chemicals and drugs which pose their own risks to worker safety. Chemicals need to be individually assessed using COSHH forms – also to be completed by the project supervisor. More information on Risk and COSHH assessments can be obtained from Mark Houghton (x45355). 3. On completion of the risk assessment form and during the course of relaying information, if the worker/ applicant gives information which suggests that they may suffer from allergic reaction to, for example, animal exposure (or any other allergen), then it is the duty of the supporting staff in Blue Block to inform the worker/ applicant that it may not be possible for them to commence work at all in the Blue Block area. A further appointment will be made for them to attend a medical examination at the Occupational Health Department to investigate further the nature of the allergen problem. Provided that the Occupational health Nurse is satisfied that the worker/ applicant will not be at risk, then the Head of Department of Physiology will be notified and then, formally, the worker will be allowed to commence laboratory work. This procedure must be followed before any laboratory work can take place. General Procedures The First Aid box for Blue Block is located on the wall between Room's 1.09 & 1.11. Additional First Aid supplies can be obtained from Mrs Helen Davies (x44719). The wearing of White laboratory coats is compulsory in all laboratories in Blue Block (important exception: see the section on animal handling area in this chapter). White coats are issued by the Department every Tuesday morning and the washing of coats is also arranged by the Department (please do not remove coats from the Department for washing at home). Further information regarding the distribution and weekly collection of white coats can be obtained from Mrs Marie Johnson (Tel ext. 45335). The consumption of food/drink is strictly forbidden in areas where experimental work is being carried out. Eating and drinking should be confined to the Staff Common Room or assigned office areas. Staff working in Blue block will be asked to sign a list acknowledging that they have received a listing of laboratories and offices where eating/ drinking restrictions are applicable. The Animal Handling Area An area of room 1.03 in Blue Block has been assigned suitable for schedule 1 killing of mice/rats. There are a number of strict guidelines which must be observed before any work can be commenced in this area. Each worker is given a safety information package on arrival in the department. Staff expected to work with animals and who suffer from allergen sensitivity, will be asked to complete a questionnaire. This is a requirement by law. More information may be obtained from the document 'Code of Practice for Allergy to Laboratory Animals', available from Mr Mark Houghton in Blue Block. Important: regardless of a workers involvement in schedule 1 handling procedure, all users of Rm 1.03 prep room must be assessed (for allergen sensitivity and experimental risk assessment) due to the proximity and potential exposure to airborne allergen. For more information contact Mr Mark Houghton. The correct techniques for animal handling will be taught during a teaching session arranged for the worker by Mr Mark Houghton. The teaching session will be carried out by Mr David Trafford from the Animal Unit. All animal handling and related work must be carried out in the approved fume hood in Room1.03. The fume hood must remain ON at all times (i.e. 24hrs). Nitrile gloves and masks must be worn by those handling animals (These will be provided). Colour-coded Navy Blue laboratory coats are supplied to be worn exclusively in Room 1.03. This is to minimise the spread of allergens. Regardless of whether staff are handling animals or undertaking general laboratory tasks in this room, you MUST wear a Navy Blue laboratory coat. Before entering the room, you must leave your white laboratory coat on the hangers provided outside the room. The Navy coats are collected for cleaning every Friday at 2.00pm. Under no conditions must white coats be allowed to come into contact with navy coats. Before leaving Room 1.03, and having completed your animal work, hands should be washed thoroughly at the hand basin within Room 1.03 (not the main prep room sink). All used blue coats must remain in Room 1.03. Waste associated with animal use (contaminated paper, animal tissue etc) should be disposed of in the yellow plastic bags provided and stored temporarily in the deep freeze (Rm 1.04). For further details about disposal contact Mr Mark Houghton. Disposal of waste paper should be in the bins clearly labelled as „Paper Only'. Likewise, disposal of glass should be in the blue 'Glass Only' bins. Procedure for ordering animals---See Mark Houghton Solvent Storage in Blue Block Organic Solvents used in Blue Block are predominately alcohols - Ethanol, Methanol. Both are stored in the Solvent locker, Rm 1.08. A key can be obtained from Mark Houghton. Naturally, it will be expected that all staff observe the common rule of not storing inorganic acids (Hydrochloric acid, Nitric acid in the Blue Block area) in the same confined area as alcohols. Genetic Manipulation Work The designated area for Genetic Manipulation is Room 1.08, which has been authorised for Containment Level 2. All workers hoping to work in this area must first be instructed in Genetic Manipulation laboratory techniques (contact Drs Oleg and Julia Gerasimenko). Before commencing any work in this area, please consult Prof Alexei Tepikin or Dr Oleg Gerasimenko. Confocal Microscopy Special microscope systems utilising Confocal technology are currently in daily use in the following areas of Blue Block: - Rm 1.16B (this room is situated inside Rm 1.04) Rm 1.13 Rm 1.07 The nature of Confocal Microscopy requires the use of Laser beam technology which is highly sensitive to vibration and potentially hazardous if exposed through accidental mishandling. For this reason, only staff authorised by Prof Alexei Tepikin may be allowed in these rooms. In Prof Tepikins' absence, permission may be sort from Dr Oleg Gerasimenko (however potential users have to receive some training from the departmental Laser Officer (contact Mark Houghton for the latest information on staff appointed for this purpose). During periods of Maintenance of the laser beam units, the immediate areas affected will have restricted access and all staff working in the area will be notified. Risk, COSSH Assessment forms and Chemical Safety Data Sheets Copies of Risk Assessments, COSHH Assessment sheets and Safety Data Sheets for all chemicals used in Blue Block area are kept in room 1.04, on the top shelf above the desk of Mark Houghton. Documentation referring to all safety matters for the Blue Block area. Centrifuges, Autoclaves The Centrifuge room is located on the 1st floor next to the cold room. Workers must be instructed how to use the centrifuge correctly by an authorised user. For Blue Block the authorised user is Dr Julia Gerasimenko. On completion of training, the worker will be included on a list of authorised Departmental centrifuge users. The autoclave room is situated on the first floor and located within the Tissue Culture Suite. Workers must be instructed how to use the autoclave correctly by an authorised user. For Blue Block the user is Dr Julia Gerasimenko. Accident and incident reporting A report form should be completed for every personal injury caused as a result of the work you are doing or by University property. A copy of this report form will be sent to the University Safety Advisor. The form can be obtained from Mrs Helen Davies. Safety Notices Notices regarding current safety information can be found on the notice board on the left hand wall after entering room 1.04. SAFETY CODE OF PRACTICE FOR THE WHITE BLOCK NEUROTRANSMITTOR BIOLOGY GROUP (LABS A11, A12, A15, A16) WORKING WITH GENETICALLY MODIFIED ORGANISMS. 1.a) Prior to commencement of any experimental work all staff should receive approval from the local GM committee for the work they are proposing to undertake. This is a legal requirement and mandatory. Adequate time should be allowed for approval to be given, bear in mind that fees may be payable and approval needed from the HSE if working at levels higher than Class I. COSHH risk assessment forms for work with biological agents (and carcinogens if relevant) should also be completed. These forms should be regularly updated as the project evolves and further approval sought where necessary. b) All staff must be trained and approved competent in good microbiological practices (see appendix) by the Principal Investigator responsible for their work. 2. The areas in the laboratory suite where genetically modified organisms can be handled are: Lab A15, Containment level 1: Live E.coli derivatives may be handled here, once the bacteria are lysed and therefore dead small-scale cultures (<10mls) may be handled in A11 and A12. The exception is work involving naked oncogenic DNA (this does not include sub-fragments of oncogenic DNA which do not have the potential to transform/immortalise or allow escape from normal growth control or induce anchorage-independent growth) this must always proceed in the designated areas. All large-scale culture work and processing must be completed in A15. Lab A16, Containment level 1: All genetic modification of cultured cells must take place here. Work involving animals: Although there are no facilities within the Laboratory Suite itself for animal work, Principal Investigators of projects involving genetic modification of animals should complete a risk assessment form. Animals that satisfy the condition that they are as safe in the containment facility (i.e. the animal house) as any recipient or parent non-manipulated animal may be maintained in one of the University Animal Facilities subject to consultation with the staff responsible for these facilities. Note that the requirements of the Animals (Scientific Procedures) Act, 1986, must be observed. If the work is to proceed at a higher containment level i.e. Higher than Containment Level 1, individuals must consult the BSO and principal Investigator to identify suitable areas at the correct containment level. 3. General rules when working with genetically modified organisms. (a) The laboratory door of the containment facility must be closed when work is in progress. (b) Laboratory coats (Howie style) or gowns must be worn in the laboratory and removed when leaving the laboratory suite. (c) Eating, chewing, drinking, smoking, storage of food or applying of cosmetics must not take place in the laboratory. (d) Mouth pipetting must not take place. (e) Hands must be disinfected or washed immediately when contamination is suspected, after handling viable materials, and also before leaving the laboratory. The elbow-operated taps must be used when working at level ACGM 2. (f) All procedures must be performed so as to minimise the production of aerosols. Biohazard containment caps must always be used where supplied when centrifuging biological/genetically modified material. (g) In the event of spillage, all contaminated surfaces must be decontaminated with a 1:50 dilution of Trigene. (working solution stable for 12 months). (h) Bench tops and Class II hood must be cleaned before and after use. All equipment must be removed from the Class II hood. (i) Used glassware and other materials should be immediately disinfected (1:50 dilution of Trigene) in the appropriate container. Pipettes must be totally immersed. Safety protocols for the Red Block Laboratories supervised by Prof. R D Burgoyne and Prof. Alan Morgan General Laboratory Guidelines Laboratory Procedures for use of Isotopes in G39 Code of Practice for Genetic Manipulation Experiments Animal Exposure Red Block Laboratories General Laboratory Guidelines 1. Lab coats should be worn at all times while carrying out experimental work. 2. Hands should be washed when experimental procedures are completed. 3. No food or drinks should be consumed or stored in any laboratory areas in Red block. 4. All work areas should be tidied after use and at the end of each day. Broken glassware, plastic pipettes or scalpel blades should be disposed of in appropriate designated containers; all used glass and plastic ware transferred for washing and benches cleaned. 6. All containers with chemical solutions or solvents should be clearly labelled. 7. In the event of spillages in specified work areas or in any general work area contaminated benches and equipment should be cleaned immediately. Any spillages on the floor should also be attended to. 8. If chemicals are to be used or procedures undertaken for which a specific risk assessment has been prepared all aspects of the assessment should be adhered to. All workers of the group will be provided with copies of all risk assessments. 9. In all cases where any potentially biohazardous reagents are used the following should be adhered to. i) Disposable gloves should be worn. ii) Stock solutions should be prepared in the fume cupboard in the preparation room (G31). Work with high concentrations of any volatile substances should be carried out in a fume cupboard. iii) Any contaminated materials should be disposed of in yellow bags for incineration. Contaminated glass and plastic ware should be soaked in 10% bleach and washed before re-use. iv) Any spillages should be dealt with immediately and these and any other accidents reported to Professor R D Burgoyne or Geoff Williams and the Laboratory Office (Len Lucas). v) Disposal of any chemicals or solvents must only be done after consultation with Geoff Williams about the correct procedures. 10. Packaging and boxes from deliveries should immediately be put in bins or placed on the floor next to bins for removal. 11. Work with radioisotopes will only be carried out in room G39 by registered personnel after appropriate training and in accordance with local regulations and Red Block Laboratory practice for room G39. 12. Work on genetic manipulation will only be carried out in room G38 by designated staff on appropriate projects after training in accordance with Red Block Laboratory Practice for room G38. 13. Any proposed laboratory work outside of standard working hours (8.00am - 6.30pm) should have the prior approval of Professor R D Burgoyne. 14. A file on specific risk assessments, safety procedures and safe disposal techniques will be maintained by Geoff Williams and available in the Central Laboratory area of Red Block. 15. Centrifuges should only be used after training in their correct use. 16. A Guide to first aid will be posted in Red Block Central Laboratory. 17. All new members of the laboratory will be given these guidelines and appropriate instructions in safety measures to be used. 18. Members of other groups may only use equipment in Red Block Laboratories with the prior permission of Professor R D Burgoyne. It is the responsibility of all members of the laboratory to safeguard themselves and others by respecting the above procedures. Laboratory Procedures for the use of Radioisotopes in G39 (Red Block) 1. Clearly labelled (including date of delivery) radioactive isotope stocks should be stored in a sealed container in the designated fridge or –20°C freezer in G39. 2. Disposable gloves (preferably two pairs) and a G39-designated lab coat (fully buttoned to the top) should be worn at all times in G39 when performing any experimental work involving radioisotopes. 3. All experimental work should be carried out over a plastic spill tray (with appropriate shielding if required) in G39. Scintillant should be stored and dispensed only in the fume cupboard in G39. 4. A full record of radioisotope stock should be kept and updated after every experiment. Individual stock sheets for each isotope can be found in the black ring binder in G39. 5. A record of any liquid waste disposed of via the designated radioactive disposal drain should be kept on the appropriate sheet in the black ring binder. During liquid waste disposal in sinks, a good water flow (avoid splashing) should be maintained to promote fast removal of the waste. 6. Experimental radioactive waste including pipette tips, culture dishes and stock bottles should be placed in the radioactive solid waste bin (yellow bin under bench in G39) and the amount of radioactivity recorded on the appropriate sheet in the black ring binder. If waste is highly radioactive (eg. 32P), it should be placed in the Perspex bin on the bench for decay prior to placing in the yellow bin. When the yellow bin is full it should be sealed with the supplied lid and placed in the radioactive bunker in the Physiology car park and the completed waste sheet given to Len Lucas. Len will issue a new bin. 7. Scintillant waste should be disposed of in the yellow scintillant waste bin under the bench in G39 and the amount of radioactivity recorded on the appropriate sheet in the black ring binder. When the bin is full, disposal of the bin should be carried out as described for the solid waste bin in (6). 8. Contamination checks (particularly for beta and gamma emitters) must be carried out before and after each radioactive experiment in G39 with a Geiger-counter for beta emitters, scintillation probe for gamma emitters and swab tests for low energy beta emitters. Monthly swab tests of all work areas should be carried out. All contamination readings must be entered on the appropriate sheet in the black ring binder. If required, contaminated plastic spill trays should be replaced and any other areas or equipment (particularly the barrels of pipettes) contaminated should be decontaminated to an acceptable level using Decon-90. 9. All benches should be cleaned after use and plastic and glassware tidied away. 4. Laboratory Practice for experimental work at level 1 in Red Block Laboratories A copy of these instructions will be posted in GM rooms 4.1 Eating, chewing, drinking, smoking, storing of food, application of cosmetics must not take place in any GM room. Mouth pipetting must not take place. 4.2 The door to GM rooms must be clearly marked with a Biohazard sign and an indication of the containment level. 4.3 The laboratory door should be closed when work is in progress. 4.4 Laboratory coats should be worn in GM rooms and removed when leaving the room. Lab coats should be buttoned all the way up. 4.5 Hands must be disinfected or washed immediately when contamination is suspected, after handling viable materials, and also before leaving the laboratory. 4.6 All procedures must be performed so as to minimise the production of aerosols. 4.6 Disinfectants must be available for immediate use in the event of spillage. 4.8 Bench tops should be cleaned and disinfected after use (gloves should be worn) and all work surfaces kept clean and tidy. Workers should monitor the state of the floors and walls to check that they are not dusty. Used glassware etc. should be placed in the appropriate disposal bins and not stored on work surfaces. 4.9 Disposable gloves should be worn at all times whilst handling potentially hazardous material and these should be discarded in the bag for autoclavable waste. 4.10 Contaminated glassware should be autoclaved. 4.11 Glass and plastic pipettes should be placed tip down until fully immersed in disinfectant and should remain immersed for at least 24 hours before removal and cleaning. Alternatively solid waste such as plasticware can be placed in yellow bags and sent for incineration. 4.12 Liquid waste should be inactivated by addition of the appropriate concentrations of powdered Virkon, Trigen E or other approved disinfectant overnight before disposal in sinks. 4.13 Waste bags should not be allowed to become more than 3/4 full before disposal. 4.14 Hand wash sinks should be used for this purpose only and the taps on these sinks operated using elbows or wrists. 4.15 Professor R D Burgoyne and Mrs Helen Davies should be informed of any accidents or untoward incidents. Red Block Procedures relating to animal exposure 1. All workers will be informed of the possible risks of development of animal allergy. 2. No one with previous experience of animal allergy of any kind will handle animals or enter rooms used for animal work. 3. Any evidence of allergic symptoms should be reported to Professor R D Burgoyne and Geoff Williams immediately. 4. Animals will be killed in the Duncan Biomedical Sciences Unit. 5. Workers handling animals will wear lab coats reserved for this purpose and gloves. Disposal ori-nasal masks will be available. 1.A LABORATORY SAFETY POLICY FOR S. WRAY’S GROUP This laboratory will try to provide, so far as is reasonably practicable, a safe and healthy working environment. Thus all members of the laboratory must play their part in achieving this, and I will be responsible for overall implementation. The personnel of the laboratory and their duties will change from time to time, but appendix 1 shows the current organisation. I will from time to time carry out “health and safety tours”. The laboratories “health and safety consultative group” will for the foreseeable future involve all research personnel including technicians and post-graduate but not under-graduate students. Visitors may be co-opted if it is felt by the group to be helpful. This group will discuss, review and plan safety within the laboratory. The group secretary will take minutes of the meeting and help generate any necessary reports. 1.B LABORATORY GENERAL CODE OF PRACTICE 1. All departmental and University safety policies and practices apply to all people working in the laboratory, no matter how brief the period. 2. Nobody can work in the laboratory without my knowledge or permission. If you would like to use a piece of equipment in somebody else‟s laboratory, you must consult the laboratory head first and be instructed in its safe use. Always be thoughtful and courteous about the use of shared and other people‟s equipment. 3. The laboratory‟s specific code of practice for working with human tissue, animals and chemicals are available to all lab members and you should ensure you have been given a copy of the appropriate codes 4. Any comments or questions about the laboratory‟s safety practices can be brought to my attention at any time. Some matters, e.g. accidents, bites must, in any case be reported. In my absence you can consult or report matters to any other member of the safety committee. There will be a group* safety meeting usually every month to review practices and matters arising. *(This is the “health and safety consultative group”) 5. Under-graduates may not work on laboratory equipment unsupervised outside of normal working hours (9.00-6.00). Other laboratory workers can work outside these times to perform routine experiments, computing and so forth. It is not advisable to change or move gas cylinders if you are on your own. You should sign the departmental “out of hours working” book which is kept in the entrance. You may wish to let security know when you arrive and leave. See also departmental information on burglar alarm. 6. All communal areas must be kept safe and tidy. You have a duty to leave these areas safe so that somebody else can come and use them and not be endangered. Any spills e.g. on benches, balances, set ups, must be dealt with in the appropriate manner. It is not acceptable, for example, to leave the balance and area around it, dirty. There will be a 10-30 minute tidying session for all lab members every week following the lab meeting. A rota for specific tasks e.g. balances, bench coats is in operation and must be complied with. 7. Do not leave solutions in glassware you wish to have cleaned. Never leave unmarked containers around which contain substances that could be harmful to others e.g. acids or alkalis. Clearly mark containers containing such solutions. Hot plates which are not in use, but hot, should have the “hot” notice placed by them. 8. Always have the safety of others and yourself in mind. 9. Keep as tidy working conditions as you can around your own area. In darkened labs it is obviously important that nothing is left on the floor, sticking out and so forth. These labs should not be used as thoroughfares. 10. All chemicals must be returned to their correct place of storage 11. All sharps and broken glass must be disposed of in the appropriate manner. The sharps bins are not for rubbish. The Blue glass bin is only to be used for placing broken glass in. 12. It is your responsibility to read the information coming with chemicals you are using. You should note also the safety instructions and code of practice for the laboratory. 13. Do not let electrical cables trail. If you are concerned about a piece of electrical equipment, see Bill Franks or the departmental electrical technician, Steve Bainbridge. 14. Use the trolleys provided for transporting cylinders. These should always be secured when in use. There are data sheets available on the safe handling, transporting and storage of gas cylinders. Never over-tighten regulator valves. Never raise sparks when fitting or opening oxygen cylinders. 15. All accidents are to be reported to the departmental safety officer and also written in the “accident report” book in MrsHelen Davies‟ office. 1. Appendix 1 RESPONSIBILITIES FOR HEALTH AND SAFETY University ↓ School (Safety Committee) ↓ Group S. Wray ↓ ↓ ↓ ↓ ↓ General Chemicals and Human Animals Policy specific assessment Tissue and tissue | | | | Susan Wray Bill Franks Bill Franks Dr R Floyd Bill Franks Karen Nobel Ted Burdyga Bill Franks ANIMAL TISSUES* 1. The University’s code of practice on laboratory animals shall be given to each member of the laboratory involved in using animals, and they should familiarize themselves with it. 2. All killing and gross dissection of animals will normally be carried out in the appropriate rooms in the University animal house. Only in exceptional cases will animals be brought to the lab e.g. for in vivo experiments. When animals need to be brought to the lab then they should be transported and kept in cages which are fitted with filters (if available) otherwise covered e.g. in plastic sacs, to prevent the spread of hair and allergens. The transporting cage should either be quickly returned to the animal house or stored in a cupboard – this will help prevent the spread of allergens. Stored cages must be returned on the next working day, at the latest. 3. Separate lab coats should be worn when dealing with animals (e.g. transporting and handling) from those worn for experimental procedures. The “animal” lab coats should be hung in the animal room when not being used. You must not enter other areas wearing them. 4. Work with animals can only be carried out in the designated laboratory area. Access to the room shall be limited to authorised persons. Animals should be handled only in the designated area. 5. Animal carcasses and tissues should be placed in yellow disposal bags before removal of incineration. When necessary these can be placed in the marked area in the fridge, in the designated laboratory area, for later removal. This should not however be used as a long-term storage facility. Animal tissue may be stored in the fridge for 24 hours if suitably labelled (date, initials). Weekly wiping and disinfecting of the fridge/freezer must be carried out. Yellow bags will be removed weekly (currently Tuesday p.m./Wednesday a.m.) by Mr. Franks. 6. Hands must be washed immediately after handling animals or animal waste. Work areas must be kept clean and regularly disinfected. Play your part in keeping the fridge clean and removing all yellow bags and pots. 7. Eating, chewing, drinking, smoking, storing of foods and application of cosmetics must not take place in the animal room. 8. Any accidents e.g. bites, must be reported. Keep your tetanus inoculation up- to-date. 9. Any new member or working visitor to the laboratory must gain instruction from animal house staff (Mr. Dave Trafford) on the anaesthetizing and killing of laboratory animals, and must never undertake these procedures alone and un- supervised until such time as they are judged competent by him and another laboratory member. Mr. Trafford holds frequent training session for new group members. If there are any concerns Professor S. Wray must be appraised. Animals must be handled gently (* Containment level 1 assumed) 10. The risk of developing allergies to animals may be reduced by wearing appropriate protective clothing e.g. goggles and facemask. Further information can be obtained in the University‟s Code of Practice for laboratory animal allergy and from the University Safety Officer. 2.B WORKING AND SAFETY PRACTISES FOR ANAESTHETISED ANIMALS 1. This work may be carried out only by Home-Office personal licence holders, who have (or who work for the holder of) the appropriate project licence, and all necessary conditions and rules must be strictly adhered to. 2. The University‟s code of practice on laboratory animals and allergies should be read and familiarized. 3. The laboratories general safety precautions for working with animals and animal tissue must be read and adhered to. S. WRAY 3. SAFETY PRECAUTIONS FOR HANDLING OF HUMAN UTERINE AND URETERIC SAMPLES* 1. Transport, storage and use of samples should be carried out in glass and plastic ware specifically designated for human sample work. 2. Separate dissecting kit for use with human samples should be used. 3. Any preparation of samples should be carried out in designated areas, clearly marked with biohazard warning tape. 4. Vinyl gloves and laboratory coats (side or back fastening) must be worn in the laboratory and removed when leaving the laboratory site. 5. Hands must be washed only in designated sink, which are those fitted with taps of a type which can be operated without being touched by hand. 6. Effective disinfectant must be available and used to disinfect bench surfaces, dissecting equipment and glassware after use. 7. Samples for disposal should be placed in the clear incineration bag, which is kept in the fridge, before removal for incineration. These should only be put on the designated shelf in the fridge. Mr. Franks will remove samples on a regular basis in accordance with the human materials regulations. 8. Eating, drinking, smoking, storing of food and applying of cosmetics must not take place in the laboratory. 9. Persons involved with handling of human samples must be familiar with the booklet “Categorisation of pathogens according to hazard and categories of containment” issued by the Advisory Committee on Dangerous Pathogens. This is available in Mrs Davies‟ office 10. Hepatitis B vaccination is strongly encouraged. S. WRAY (*Containment level 2 assumed) 4. USE OF CHEMICALS – SAFETY PRECAUTIONS AND CODE OF PRACTICE 1. The Control of Substances Hazardous to Health (COSHH) regulations require that assessments are made of every use of a substance hazardous to health. 2. In many cases in this laboratory this can be done by standard precautions. These can be determined by all graduates. For example should you use a fume cupboard, or can open use be made of them? If you are in any doubt, consult me or a member of the departmental safety committee 3. Where standard precautions do not apply a specific assessment is required. This should be done initially by the group member using the chemical but then be brought to me for authorization. If you require help, consult me or a member of the departmental safety committee. Mr. Franks has compiled our specific assessments, which are date numbered for chemicals. These are kept in a labelled file. Mr. Franks also has data sheets, filed in alphabetical order, on all the other chemicals we hold, which are governed by standard precautions. You should be familiar with where these two files are kept. You may not remove them or items from them. Please also read the “safety data sheet” which is attached as appending 4.i. 4. When laboratory members start an experimental protocol they should list in the back of their lab notebooks, all chemicals to be used. You should then determine whether standard precautions apply or if a specific assessment is required. It is suggested that you keep your own additional copy, of any specific assessments that relates to your work, in your lab notebook. 5. Use of carcinogens, teratogens or embryotoxins always requires a specific assessment. Once I have approved this or drawn this up, it has to be approved by the departmental safety officer. No work using substances in this category can be started until this approval is given. 6. Details and amplification of the above can be found in the University‟s “control of substances hazardous to health – carrying out assessments” leaflet in Mr. Lucas‟ office. 7. Do not transfer chemicals to unlabelled containers. If you do transfer chemicals to another container always label it with any hazardous warning information. If you provide others with chemicals, always provide the information on hazards. If you obtain chemicals from others always ask about hazard information; if necessary contact the supplier. 8. Mouth pipetting is not permitted. 9. Eating, chewing, drinking, smoking and application of cosmetics are not permitted in laboratories. 10. At the end of the laboratory session, wash your hands. 11. If necessary wear eye protection, or a full-face visor. For substances, which are harmful in contact with the skin, use the appropriate gloves. 12. Tops should be quickly replaced on all volatile chemicals. When not working in a fume cupboard try and keep apparatus enclosed e.g. keep a top on the anaesthetizing jar. Take care not to inhale or breathe the atmosphere very close to the point of release of a substance. Less than 500ml at a time should be kept on the open bench if at all possible when using flammable liquids 13. See the separate sheet appendix II for incident advice. 14. Chemicals and waste should be disposed of in the appropriate manner. Contact the departmental safety officer if you are unsure. S. WRAY 4. Appendix i SAFETY DATA SHEETS 1. These are kept in a file clearly marked, near to the main chemical shelves. The Sigma Data Sheets give all the code numbers. 2. For the safe handling of the chemicals the code numbers and their definitions are in the front of this folder. 3. The list is in alphabetical order and includes all the chemicals currently in use. It will be periodically updated, but if you think that something is missing or that more information is required about the chemicals or compounds you are working with, then you should make your views known to either me or Mr. Franks. 4. In the data sheets are B.O.C. notes on the safe handling, transporting and storage of their gas cylinders. Do not over tighten a regulator valve on a cylinder and do not use anything that may raise a spark when fitting or opening a regulator on an oxygen cylinder. 5. There is a list of the products supplied to us from Molecular Probes, Calbiochem, Radio Spares etc – if you feel that any of this information is incorrect or out-dated could you raise this matter with me or Mr. Franks. 6. Individual company web sites often have the latest Safety Data Sheets for products we are using or may use in future planned experiments. These web sites are “book-noted” on „Smithers‟. 4. Appendix ii EMERGENCIES AND ACCIDENTS Telephone 2222 for fire, police, ambulance, bomb threats and emergencies. Safety Advisor: 43242, 43243, and 43244. Security 43252, 43249. When fire alarm sounds you must evacuate the building. Mr Franks to check personnel have complied Users of substances hazardous to health should know what they would do in the event of a foreseeable contingency. Spillage In the event of a spillage outside a fume cupboard or local exhaust enclosure: If the substance is flammable, eliminate sources of ignition. Instruct others to keep at a safe distance. Open windows where appropriate. For spillage of volatile materials, evacuate the room and shut the door unless the substance has been determined as safe to use in the open. Unless the spillage is one which may be left to clear by evaporation and ventilation, it should be cleared up by at least two people wearing appropriate masks absorbing it onto sand or proprietary absorbent which is transferred into a container which is put into a well ventilated space for ultimate disposal. Toxic Gas Leakage If compressed gas is leaking and cannot be shut off, feed the gas if possible into a fume cupboard or other ventilation discharge directly to the outside. Where practicable transport a leaking cylinder to the open air and clear the surrounding area. If this is not practicable, evacuate the room, if possible opening windows before leaving. Subsequent removal of the cylinder to a better ventilation place should only be carried out by at least two people wearing appropriate masks. Reporting Report accidents and incidents on a University Accident/Incident Report form. Wherever there is reason to believe that someone has had a higher than acceptable level of exposure to a substance it should be reported to the Safety Advisor and to Occupational Health. Anyone who thinks their health might have been affected by exposure to substances at work (including e.g. dermatitis or asthma) should contact Occupational Health. School of Biomedical Sciences Division of Physiology Safety protocols for the 4th Floor, Nuffield Building Laboratories supervised by Drs Doherty, Mora, Plagge, Royle and Wilm General Laboratory Guidelines Laboratory Procedures for use of Radioisotopes in 4.12 and 4.13 Code of Practice for Genetic Manipulation Experiments Animal Exposure Specific Risk Assessments May 2007 Laboratories on the 4th Floor, Nuffield Building General Laboratory Guidelines 1. Howie style Lab coats should be worn at all times while carrying out experimental work. 2. Hands should be washed when experimental procedures are completed. 3. No food or drinks should be consumed or stored in any laboratory areas. 4. Eating and drinking are allowed in 4.01, 4.02, 4.07, 4.08, 4.09, 4.10, 4.16A. Food or drink may be transported through a laboratory but the food or drink must be sealed, e.g. mugs of coffee without a lid must not be carried through. Under no circumstances will washing up of crockery and glassware used for eating and drinking be carried out in laboratory areas. Food preparation and washing up facilities can be found on the third floor adjacent to the common room. 5. No mouth pipetting must be carried out. 6. All work areas should be tidied after use and at the end of each day. Broken glassware, plastic pipettes or scalpel blades should be disposed of in appropriate designated containers, all used glass and plastic ware disinfected and transferred for washing and benches cleaned. 7. All containers with chemical solutions or solvents should be clearly labelled. 8. In the event of spillages in specified work areas or in any general work area contaminated benches and equipment should be cleaned immediately. Cleaning procedures for specific chemicals should be outlined in the COSHH forms. It is your responsibility to know how a chemical should be cleared up should spillage occur. Any spillages on the floor should also be attended to. Mop up excess liquid and notify others if the floor is wet. 9. If chemicals are to be used or procedures undertaken for which a specific risk assessment has been prepared all aspects of the assessment should be adhered to. All workers of the group will be provided with copies of all risk assessments. 10. In all cases where any potentially biohazardous reagents or dangerous chemicals are used the following should be adhered to. i) Appropriate single use gloves should be worn. ii) Stock solutions should be prepared in a fume cupboard. Work with high concentrations of any volatile substances should also be carried out in a fume cupboard. iii) Any biologically contaminated materials should be disposed of in yellow clinical waste bags for incineration. Contaminated glass and plastic ware should be soaked in 10% bleach or Virkon/Trigene for at least 15-30 minutes and washed before re-use. Waste from GM experiments (e.g. agar plates) should be collected in an autoclavable bag, placed in a biowaste bin and sealed using autoclave tape for autoclaving. The bin prevents any leaks and allows for easy transport to the autoclave. iv) Any spillages should be dealt with immediately and these and any other accidents reported to a group leader and the Laboratory Office (Len Lucas). v) Disposal of any chemicals or solvents must only be done after consultation about the correct procedures. vi) There is currently no liquid nitrogen store on the 4th floor. Small amounts of liquid nitrogen may be brought in using an appropriate dewar with a lid. Refer to the University of Liverpool code of practice on physical hazards before commencing work with liquid nitrogen. vii) Before work can begin in a microbiological safety cabinet (Rooms 4.11 and 4.12), fume hood (Rooms 4.13 and 4.24) or laminar flow cabinet (4.12), users MUST receive training on appropriate use from a group leader. Be aware of the level of protection that each safety cabinet provides. 11. Packaging and boxes from deliveries should immediately be put in waste paper bins or placed on the floor next to bins for removal. Large pieces of cardboard can be temporarily placed in the foyer area for collection by Michael Leahy. 12. Work with radioisotopes will only be carried out rooms designated for radioactive work by registered personnel after appropriate training and in accordance with local regulations and 4th Floor practice. 13. Work on genetic manipulation will only be carried out by designated staff on appropriate projects after training in accordance with 4th Floor Practice. 14. Any proposed laboratory work outside of standard working hours (8.00am - 6.30pm) should have the prior approval of a group leader – and be recorded in the out of hours book. 15. A file on specific risk assessments, safety procedures and safe disposal techniques will be maintained and made available in the in 4.07. 16. Centrifuges should only be used after training in their correct use. 17. A Guide to first aid will be posted in 4.07. 18. All new members of the laboratories will be given these guidelines and appropriate instructions in safety measures to be used. 19. Members of other groups may only use equipment in 4th Floor laboratories with the prior permission of the appropriate group leader. It is the responsibility of all members of the laboratory to safeguard themselves and others by respecting the above procedures. Laboratory Procedures for the use of Radioisotopes in 4.12 and 4.13, 4th Floor, Nuffield Building. 10. Clearly labelled (including date of delivery) radioactive isotope stocks should be stored in a sealed container in the designated fridge or –20°C freezer in 4.13. 11. Rubber disposable gloves (preferably two pairs) and a 4.12/4.13- designated lab coat (fully buttoned to the top) should be worn at all times in 4.12 and 4.13 when performing any experimental work involving radioisotopes. 12. All experimental work should be carried out over a plastic spill tray (with appropriate shielding if required) in 4.13. Scintillant should be stored and dispensed only in the fume cupboard in 4.13. 13. A full record of radioisotope stock should be kept and updated after every experiment. Keep records of all radioisotope usage and disposal in the appropriate sheet. Individual stock sheets for each isotope can be found in 4.13. 14. A record of any liquid waste disposed of via the designated radioactive disposal drain should be kept on the appropriate sheet in the radioactivity folder. During liquid waste disposal in sinks, a good water flow (avoid splashing) should be maintained to promote fast removal of the waste. 15. Experimental radioactive waste including pipette tips, culture dishes and stock bottles should be placed in the radioactive solid waste bin (yellow bin in 4.13) and the amount of radioactivity recorded on the appropriate sheet in the black ring binder. If waste is highly radioactive (eg. 32P), it should be placed in the Perspex bin on the bench for decay prior to placing in the yellow bin. When the yellow bin is full it should be sealed with the supplied lid and placed in the radioactive bunker in the Physiology car park and the completed waste sheet given to Len Lucas. Len will issue a new bin. 16. Scintillant waste should be disposed of in the yellow scintillant waste bin under the bench in 4.13 and the amount of radioactivity recorded on the appropriate sheet in the black ring binder. When the bin is full, disposal of the bin should be carried out as described for the solid waste bin in (6). 17. Contamination checks (particularly for beta and gamma emitters) must be carried out before and after each radioactive experiment in 4.13 and 4.12 with a Geiger-counter for beta emitters, scintillation probe for gamma emitters and swab tests for low energy beta emitters. Monthly swab tests of all work areas should be carried out. All contamination readings must be entered on the appropriate sheet in the radioactivity folder. If required, contaminated plastic spill trays should be replaced and any other areas or equipment (particularly the barrels of pipettes) contaminated should be decontaminated to an acceptable level using Decon-90. 18. All benches should be cleaned after use and plastic and glassware tidied away. 4. Laboratory Practice for experimental work in Laboratories on the 4th Floor, Nuffield Building A copy of these instructions will be posted in microbiological containment labs 4.1 Eating, chewing, drinking, smoking, storing of food, application of cosmetics must not take place in any GM room. Mouth pipetting must not take place. 4.2 The door to GM rooms must be clearly marked with a Biohazard sign and an indication of the containment level. 4.3 The laboratory door should be closed when work is in progress. 4.4 Howie style Laboratory coats should be worn in GM rooms and removed when leaving the room. Lab coats should be buttoned all the way up. 4.5 Hands must be disinfected or washed immediately when contamination is suspected, after handling viable materials, and also before leaving the laboratory. 4.6 All procedures must be performed so as to minimise the production of aerosols. 4.6 Disinfectants must be available for immediate use in the event of spillage. 4.8 Benchtops should be cleaned and disinfected after use (gloves should be worn) and all work surfaces kept clean and tidy. Workers should monitor the state of the floors and walls to check that they are not dusty. Used glassware etc. should be placed in the appropriate disposal bins and not stored on work surfaces. 4.9 Disposable gloves should be worn at all times whilst handling potentially hazardous material and these should be discarded in the bag for autoclavable waste. 4.10 Contaminated glassware should be autoclaved. 4.11 Glass pipettes should be placed tip down until fully immersed in disinfectant and should remain immersed for >24 h before removal and cleaning. Alternatively solid waste such as plasticware can be placed in yellow bags and sent for incineration. 4.12 Liquid waste should be inactivated by addition of the appropriate concentrations of Virkon, Trigen E or other approved disinfectant overnight before disposal in sinks. 4.13 Waste bags should not be allowed to become more than 3/4 full before disposal. 4.14 Hand wash sinks should be used for this purpose only and the taps on these sinks operated using elbows or wrists. 4.15 Len Lucas and either Dr Doherty, Mora, Plagge, Royle or Wilm should be informed of any accidents or untoward incidents. They can advise if an accident/incident form needs to be completed. 4th Floor Procedures relating to animal exposure All workers should be registered with Occupational Health and a health questionnaire completed before any work starts. All workers will be informed of the possible risks of development of animal allergy. No one with previous experience of animal allergy of any kind will handle animals or enter rooms used for animal work, unless absolutely required and appropriate countermeasures to effectively suppress allergic reactions have been taken. Any evidence of allergic symptoms should be reported to either Dr Doherty, Mora, Plagge, Royle, Wilm immediately. Animals will be killed whenever possible in the Duncan Biomedical Sciences Unit. Where this is unavoidable, animals will be brought to room 4.23, killed and tissues removed. Animals will be killed through a recognised Schedule 1 procedure by a person holding a Home Office personal licence or by a competent person, who has been properly trained in Schedule 1 procedures. A copy of Schedule 1 humane killing procedures will be available in room 4.23. If tissues are to be processed for primary cell culture, they will be rinsed in appropriate buffer (e.g. PBS) first (to minimise risk of exposure to allergens), before they are taken into room 4.12 for further processing in the flow cabinet. Workers handling animals will wear lab coats reserved for this purpose and gloves and individually face-fitted RPE according to the Code of Practice for Allergy regulations. Disposal FFP2 ori- nasal masks will be available. Carcasses and tissues will be collected in plastic bags and temporarily stored in the -20°C freezer in room 4.23 until they are centrally disposed off.
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