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Fungi Identification Test Panel FF MicroPlate

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					                                                                                                                                       Fungi Identification Test Panel
FF MicroPlate™
A1                  A2               A3                  A4                  A5                  A6                 A7                A8                A9                 A10               A11               A12
Water               Tween 80         N-Acetyl-D-         N-Acetyl-D-         N-Acetyl-D-         Adonitol           Amygdalin         D-Arabinose       L-Arabinose        D-Arabitol        Arbutin           D-Cellobiose
                                     Galactosamine       Glucosamine         Mannosamine

B1                  B2               B3                  B4                  B5                  B6                 B7                B8                B9                 B10               B11               B12
α-Cyclodextrin      β-Cyclodextrin   Dextrin             i-Erythritol        D-Fructose          L-Fucose           D-Galactose       D-Galacturonic    Gentiobiose        D-Gluconic Acid   D-Glucosamine     α-D-Glucose

C1                  C2               C3                  C4                  C5                  C6                 C7                C8                C9                 C10               C11               C12
Glucose-1-          Glucuronamide    D-Glucuronic Acid   Glycerol            Glycogen            m-Inositol         2-Keto-D-Gluconic α-D-Lactose       Lactulose          Maltitol          Maltose           Maltotriose
Phosphate                                                                                                           Acid

D1                  D2               D3                  D4                                      D6                 D7                D8                D9                 D10               D11               D12
D-Mannitol          D-Mannose        D-Melezitose        D-Melibiose                             β-Methyl-D-        α-Methyl-D-       β-Methyl-D-       Palatinose         D-Psicose         D-Raffinose       L-Rhamnose
                                                                             D5                  Galactoside        Glucoside         Glucoside

E1                  E2               E3                  E4                  E5                  E6                 E7                E8                E9                 E10               E11               E12
D-Ribose            Salicin          Sedoheptulosan      D-Sorbitol          L-Sorbose           Stachyose          Sucrose           D-Tagatose        D-Trehalose        Turanose          Xylitol           D-Xylose

F1                  F2               F3                  F4                  F5                  F6                 F7                F8                F9                 F10               F11               F12
γ-Amino-butyric     Bromosuccinic    Fumaric Acid        β-Hydroxy-butyric   γ-Hydroxy-butyric   p-Hydroxyphenyl-   α-Keto-glutaric   D-Lactic Acid     L-Lactic Acid      D-Malic Acid      L-Malic Acid      Quinic Acid
Acid                Acid                                 Acid                Acid                acetic Acid        Acid              Methyl Ester

G1                  G2               G3                  G4                  G5                G6                   G7                G8                G9                 G10               G11               G12
D-Saccharic Acid    Sebacic Acid     Succinamic Acid     Succinic Acid       Succinic Acid     N-Acetly-L-          Alaninamide       L-Alanine         L-Alanyl-Glycine   L-Asparagine      L-Aspartic Acid   L-Glutamic Acid
                                                                             Mono-Methyl Ester Glutamic Acid

H1                  H2               H3                  H4                  H5                  H6                 H7                H8                H9                 H10               H11               H12
Glycyl-L-Glutamic   L-Ornithine      L-Phenylalanine     L-Proline           L-Pyroglutamic      L-Serine           L-Threonine       2-Amino Ethanol   Putrescine         Adenosine         Uridine           Adenosine-5'-
Acid                                                                         Acid                                                                                                                              Monophosphate

INTRODUCTION                                                                 FIGURE 1. Carbon Sources in FF MicroPlate

Over the past several years, mycology has emerged as an                                                                  The FF MicroPlate employs a redox chemistry similar to
increasingly important part of the microbiology laboratory.                                                              Biolog's other proven microbial identification/characterization
Fungal contaminants can contribute to significant losses in                                                              products. This chemistry, based on reduction of tetrazolium,
food and industrial processes. Environmental monitoring over                                                             responds to the process of metabolism (oxidation of
the last several years has focused increasingly on fungal                                                                substrates). Biolog’s universal chemistry works with any
isolates as the source of conditions such as sick building                                                               carbon source and greatly simplifies the testing process, as no
syndrome. In agriculture, fungal pathogens cause serious                                                                 color developing chemicals need to be added after inoculation.
problems requiring constant attention from phytopathologists.                                                            The FF database also analyzes fungal growth via turbidimetric
In human disease, the list of fungal pathogens has grown in                                                              analysis.
the clinical laboratory due to an increased population of
immunocompromised patients.                                                                                              Analysis of both color development and turbidity provides for
                                                                                                                         extremely accurate identifications to the species level.
The Biolog FF MicroPlate (Figure 1) is the first broad based                                                             There are currently over 70,000 named species of an estimated
rapid identification and characterization product designed for                                                           250,000 species of fungi. For scientists working with fungi
filamentous fungi and yeast, including species from the genera                                                           outside the Biolog database, the FF MicroPlate and
Aspergillus, Penicillium, Fusarium, Alternaria, Mucor,                                                                   MicroLog software have been designed to allow the user to
Gliocladium, Cladosporium, Paecilomyces, Stachybotrys,                                                                   create their own database by adding the patterns produced by
Trichoderma, Zygosaccharomyces, Acremonium, Beauveria,                                                                   new cultures.
Botryosphaeria, Botrytis, Candida, and Geotrichum.
                                                                               Fungi Identification Test Panel


An important added feature of the FF Database is a unique library of macroscopic and microscopic photographs of the fungi to aid in
the identification of unknown organisms. This tool can be used to confirm the identification of unknown organisms by providing a
visual and morphological verification of each species identified. The following two blocks of photos and captions are examples taken
directly from the database software. Please note that the macroscopic photos are full color images.

                                                  Aspergillus flavus

                     (a) MA, 7 days; (b) CYA, 7 days; (c,d) conidiophores; (e) conidia.
                                             (a-e) CBS 282.95.

Explanation of caption terminology: MA= Malt Extract Agar; CYA= Czapek Yeast Autolysate Agar
CBS 282.95, DAOM 216724 and CBS 324.89 are strain reference numbers.

                       3938 Trust Way      Hayward, CA 94545       Telephone: 510-785-2564      Fax: 510-782-4639
                                                   Fungi Identification Test Panel

Penicillium aurantiogriseum var. aurantiogriseum

(a) MA, 7 days; (b) CYA, 7 days; (c,d) conidiophores; (e) conidia.
             (a,b) DAOM 216724; (c-e) CBS 324.89.

  3938 Trust Way   Hayward, CA 94545   Telephone: 510-785-2564   Fax: 510-782-4639
                                                                             Fungi Identification Test Panel

Most scientists performing identifications on fungal samples        The Biolog FF Database is the first and only product of its
still use traditional methods of macroscopic and microscopic        kind. It has the largest database of any kit-based method for
examination. The FF MicroPlate and Database provide a               the identification of filamentous fungi. This superior product
simple and accurate method as an alternative or as a                will be an invaluable addition to your microbiology
complement to these traditional methods that require a high         laboratory. Included in this database are:
degree of skill, training, and judgment.
                                                                        o    Clinically important, allergenic and mycotoxigenic
The Biolog FF MicroPlate performs 95 discrete tests                          fungi – Stachybotrys, Scopulariopsis, Paecilomyces,
simultaneously and gives a characteristic reaction pattern                   Cladosporium, Alternaria, Fusarium, Aspergillus,
called a “fingerprint”. These fingerprint reaction patterns                  etc.
provide a vast amount of information about the metabolic
properties of each fungus tested, along with a species level            o    Significant indoor air fungi – Penicillium,
identification. The FF Database contains over 500 taxa of                    Aspergillus, Eurotium, Rhizopus, Stachybotrys,
fungi from over 120 genera.                                                  Neurospora, Wallemia, etc.

                                                                        o    Environmentally important fungi – Trichoderma,
PROCEDURE FOR USING FF MICROPLATE                                            Fusarium, Mucor, Acremonium, Verticillium,
                                                                             Aureobasidium, Rhodotorula, Sporobolomyces, etc.
The procedure is fast and simple, involving only 5 steps, and
requiring only 2 to 3 minutes hands-on time per sample.                 o    Plant pathogenic fungi – Fusarium, Colletotrichum,
                                                                             Phoma, Botrytis, etc.
    1) Grow a pure culture of a fungus on a 2% Malt Extract
       Agar plate (Biolog Catalog #71106 for pre-poured                 o    Food-borne fungi – Penicillium, Aspergillus,
       plates) until enough conidiation is present to prepare                Rhizopus, Moniliella, Cryptococcus, Candida,
       a suspension.                                                         Saccharomyces, etc.
    2) Swab the conidia from the surface of the agar plate,
       and suspend to a specified density in FF Inoculating             o    Broad coverage in important genera – over 60
       Fluid (Biolog Catalog # 72106).                                       Aspergillus spp., over 80 Pencillium spp., over 80
    3) Pipet 100 µl of suspension into each well of the FF                   Fusarium spp.
       MicroPlate (Biolog Catalog # 1006).
    4) Incubate the FF MicroPlate at 26o C for 24 – 96              For more information, contact us using the information below.
    5) Read the MicroPlates using the Biolog
       MicroStation Reader beginning 24 hours after

                        3938 Trust Way       Hayward, CA 94545   Telephone: 510-785-2564      Fax: 510-782-4639