Table 1 Antibodies used for immunocytochemistry

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Table 1 Antibodies used for immunocytochemistry Powered By Docstoc
					Supplementary Data

Table 1: Antibodies used for immunocytochemistry

       Antibody         Host           Reports           Working              Source

                                                         Dilution

       Albumin          mouse         Albumin             1:10        Dako, Glostrup, Denmark

                                     production

   Monoclonal CK18      mouse       Hepatocytes           1:25        Dako, Glostrup, Denmark

   Monoclonal CK19      mouse         Bile Cells          1:50        Dako, Glostrup, Denmark

      Monoclonal        rabbit    Endothelial cells       1:200       Dako, Glostrup, Denmark

    Von Willibrand

      Monoclonal        rabbit   Epithelial cell-cell     1:100         Zymed, Carlsbad, CA

      E-Cadherin                     interactions

   Monoclonal PCNA      Clone     Proliferating cells     1:200       Dako, Glostrup, Denmark

                        PC10

      Polyclonal        rabbit     Apoptotic cells        1:50
                                                                    Biocare Medical, Concord, CA
   Cleaved Caspase 3

  Monoclonal Laminin    mouse     ECM deposition          1:100        Chemicon Temecula, CA

  Polyclonal Vimentin   mouse    Fibroblast-like cells    1:100         Zymed, Carlsbad, CA
Supplementary Data

Table 2: PCR primers used for RT-PCR and reaction* conditions

     Gene             Acc. No.                 Forward Primer         Amplicon    Annealing

                                               Reverse Primer         size (bp)   Temp (oC)

    Albumin         NM134326           5'-TTCCTGAAGCTCAGAGACTG-3'       390          55

                                       5'- TGGCGAAGCAGTTATCCTTG-3'

      TAT           NM012668          5'-ATTTCCCGGAATTCGAGAACGA-3'      234          58

                                      5'- GACGGGTGAGGGCTTATTTGTC-3'

    PEPCK             K03243           5'- CCAGCCAGAGTATATTCACA-3'      261          55

                                       5'- GCTTTCTCGAAGTCCTCTTC-3'

      AFP             M18351          5'-TGCCCGACAGAGAAAAATATG-3'       312          56

                                      5'- GCAGTTTCTGGAAAGTGGAAG-3'

    GAPDH           NM017008           5'-TGGTGCTGAGTATGTCGTG-3'        272          55

                                       5'- TGGCATGGACTGTGGTCAT-3'

      TO            NM022403           5'-TGTAGTTCAGAGACGTGATG-3'       245          50

                                       5'-GAACTGCTCACCAAGCTTTA-3'

     PCNA           NM022381           5'-TCCCAGACAAGCAATGTTGA-3'       332          51

                                      5'- AGGTACAAACTTGGTGACAG-3'

     CD34           XM223083           5'-ACTTTCCAGCAAACTCCAGC-3'       147          53

                                       5'-AGCAGGACTCCCGAGGTAAC-3'



  *All reactions involved 35 cycles