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Protein A

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					                                                          Protein A
                                                            Cat. No.        Form                                                    Quantity
                                                            7300-01S        Purified (UNLB) Protein A                               1.0 mg
                                                            7300-01L        Purified (UNLB) Protein A                               5.0 mg
                                                            7300-05         Horseradish Peroxidase (HRP) Conjugate                   1.0 ml

DESCRIPTION
Source              Staphylococcus aureus
Mr/Structure        42-kDa. Protein A is a monomeric protein lacking cysteine residues.
Specificity         Protein A binds the Fc portion of human IgG1, IgG2, IgG3 (weakly), IgG4, and mouse
                    IgG1 (weakly), IgG2a, IgG2b, and IgG3. Protein A also binds IgG from monkey, rabbit, pig,
                    guinea pig, dog, and cat.

RESEARCH APPLICATIONS
Protein A-UNLB
   • Affinity chromatography
   • Immunoprecipitation
Protein A-HRP
   • Enzyme-Llinked-Immunosorbent-Assay (ELISA)
   • Immunoblotting
   • Immunohistochemistry

CHARACTERIZATION
To insure lot-to-lot consistency, each batch of product is tested by FPLC and ELISA to conform to the
characteristics of a standard reference reagent. Protein A-HRP is prepared using highly purified
horseradish peroxidase (RZ > 3.0).

WORKING DILUTIONS
     ELISA                              HRP conjugate                                           1:4,000-1:8,000

     Other Applications:                Since applications vary, you should determine the optimum working
                                        dilution of the product that is appropriate for your specific need.

HANDLING AND STORAGE
•   The purified Protein A is supplied as either 1.0 mg or 5.0 mg in lyophilized form. No BSA, preservatives
    or amine-containing buffer salts have been added. Store up to one year at -20°C; or after reconstitution
    in phosphate buffered saline to highest concentration possible, store up to one month at 2-8°C.
•   Protein A-HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No
    other preservatives have been added. Store at 2-8°C or long-term at -20°C.
•   Reagents are stable for the period shown on the label if stored as directed.



                      For Research Use Only. Not for Diagnostic or Therapeutic Use.


       Corporate Offices: 160A Oxmoor Blvd. • Birmingham, AL 35209 • USA Mailing Address: P.O. Box 26221 • Birmingham, AL 35260 • USA
                                   Tel: 205.945.1774 • U.S. and Canada: 800.722.2255 • Fax: 205.945.8768
                                    Email: info@southernbiotech.com • Website: www.southernbiotech.com
REFERENCES
  1.    Kronvall G., U.S. Seal, J. Finstad, and R.C. Williams Jr. 1970. J. Immunol. 104:140.
  2.    Bjork I., B.A. Petersson, and J. Sjoquist. 1972. Eur. J. Biochem. 29:579.
  3.    Sjoquist J., B. Meloun, and H. Hjelm. 1972. Eur. J. Biochem. 29:572.
  4.    Ankerst J., P. Christensen, L. Kjellen, and G. Kronval. 1974. J. Infect. Dis. 130:268.
  5.    Kronvall G., U.S. Seal, S. Svensson, and R.C. Williams Jr. 1974. Acta. Pathol. Microbiol. Scand. [B] Microbiol. Immunol. 82:12.
  6.    Jonsson S., and G. Kronvall. 1974. Eur. J. Immunol. 4:29.
  7.    Hjelm H., J. Sjodahl, and J. Sjoquist. 1975. Eur. J. Biochem. 57:395.
  8.    Hjelm H. 1975. Scand. J. Immunol. 4:633.
  9.    Dubois-Dalcq M., H. McFarland, and D. McFarlin. 1977. J. Histochem. Cytochem. 25:1201.
  10.   Ey P.L., S.J. Prowse, C.R. Jenkin. 1978. Immunochemistry 15:429.
  11.   Langone J.J. 1978. J. Immunol. Methods 24:269.
  12.   Richman D.D., P.H. Cleveland, M.N. Oxman, and K.M. Johnson. 1982. J. Immunol. 128:2300.
  13.   Lindmark R., K. Thoren-Tolling, and J. Sjoquist. 1983. J. Immunol. Methods 62:1.
  14.   Chang H.C., I. Takashima, J. Arikawa, and N. Hashimoto. 1984. J. Virol. Methods 9:143.
  15.   Bjercke R.J., G. Cook, N. Rychlik, H.B. Gjika, H. Van Vunakis, and J.J. Langone. 1986. J. Immunol. Methods 90:203.
  16.   Compton B.J., M.A. Lewis, F. Whigham, J.S. Gerald, and G.E. Countryman. 1989. Anal. Chem. 61:1314.
  17.   Crichton D.N., and B.B. Cohen. 1989. Immunol. Today 10:325.
  18.   Watson P.R., T.H. Guthrie Jr., and R.J. Caruana. 1989. Cancer 64:1400.
  19.   Dean E.A., S.C. Whipp, and H.W. Moon. 1989. Infect. Immun. 57:82.




        Corporate Offices: 160A Oxmoor Blvd. • Birmingham, AL 35209 • USA Mailing Address: P.O. Box 26221 • Birmingham, AL 35260 • USA
                                    Tel: 205.945.1774 • U.S. and Canada: 800.722.2255 • Fax: 205.945.8768
                                     Email: info@southernbiotech.com • Website: www.southernbiotech.com
                                                                                                                                          7300.doc
                                                                                                                                         29-Apr-05

				
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