Absorbance Luminescence Spectra MR Technical Specifications wavelength range 200 – 1,000 nm scan rate > 90 nm / min. optical bandpass 2 nm ± 0.3 nm wavelength accuracy ± 1 nm Plate Washing photometric range 0.0 A to 4.0 A The Leading photometric resolution ± 0.001 A photometric accuracy greater of ± 0.005 or 1.0 % from 0.0 to 2.0 A 2.0 % from 2.0 A to 3.0 A Light in photometric precision < 0.005 A SD or < 1% CV (which ever is greater) between 0.0 and 2.0 A; < 2.0% CV between 2.0 and 3.0 A Microplate stray light < 0.03 % @ 230 nm Automated light source xenon flash lamp Processing temperature range ambient +3 o to 45 o C Analysis interface USB Ordering information 29010 Spectra MR Software Spectra MR TM 28040 Verification Plate Microplate Spectrophotometer Consumables Experts In Microplate Analysis DYNEX Technologies, Inc. DYNEX Technologies Limited 14340 Sullyfield Circle, Chantilly, VA 20151-1621 Columbia House, Columbia Drive TEL 800.288.2354 / 703.631.7800 FAX 703.803.1441 Worthing Contact DYNEX Technologies, Inc. West Sussex BN13 3HD UK firstname.lastname@example.org TEL: +44 (0) 1903 267555 for additional information and FAX: +44 (0) 1903 267722 www.dynextechnologies.com E-Mail:email@example.com a product demonstration at 800-288-2354 703-631-7800 Experts In Microplate Analysis Spectra MR and Revelation are trademarks of DYNEX Technologies, Inc. 95000160 All other trademarks and registered trademarks belong to their respective owners. Rev. 10/15/04 Spectra MR applications ™ Spectra MR Optical performance without compromise yields results at the highest level with a versatile range of plate formats. The spectrophotometer’s Microplate Spectrophotometry at the Highest Level Revelation Software easily determines concentrations of nucleic acids at A260, A260-A320 and A260/A280 in 96- and 384-well microplates. An extinction coefficient can be used to approximate a concentration. The path length correction function can be used to compare Spectra MR data to data from any other spectrophotometer. Multiplication factors typically used for standard 1 cm path length cuvettes can also be used for microplates with the Spectra MR. Scanning to establish the absorbance spectrum and The Spectra MR path length correction function enables results to be directly identify peak absorbance of a new dye is accomplished with ease and compared to data from any other used to achieve optimum results from endpoint assays. • Ease-of-use spectrophotometer. Clearly and conveniently organized software. Data • Precise & accurate results collection assays are created, named and stored easily in Revelation Software. • Performance verification Spectra MR Applications • Most stable platform • UV/Visible absorbance & photometry • Protein analysis • Cost-effective • Spectrum scanning Biuret protein determination • Nucleic acid analysis Bradford protein determination RNA Concentration (A260) BCA protein determination ssDNA concentration (A260) Endpoint calibration curve protein assays dsDNA concentration (A260) • Colorimetric assays DNA purity assessment (A260/A280) ELISA / EIA Immunoassays • Enzyme kinetics Kinetic ELISA / EIA E.g. Vmax and Km b-galactosidase activity • Turbidimetric detection T he Spectra MR delivers performance for the value-driven laboratory that meets • NAD(P)H-based assay • Cell proliferation Nitric oxide determination the challenges of today’s microplate photometric applications. Spectral Scans are easily performed and • Cytotoxicity can be directly exported to Excel. Saved scans can be recalled and conveniently displayed on one auto-scaled graph for The Spectra MR scanning microplate spectrophotometer Precision and accuracy without compromise comparison. affords the most stable and accurate absorbance detection Spectra MR provides accurate results quickly and precisely in a convenient, compact instrument. Spectra MR accepts with zero drift and excellent baseline performance. all microplate formats from 6 to 384-wells. Multiple Obtaining data is intuitive and is easily processed through Software that’s both easy to use and facilitates results photometric readings per well can be obtained on large RevelationTM Software. Critical analyses can be performed, The Revelation Software platform is a well-established tool for obtaining format plates at wavelengths from 200 nm to 1,000 nm. including endpoint quantitative assays and enzyme kinetics. data, calculating and interpreting absorbance results. Features include easy Data can be directly exported to Microsoft® Excel or easily creation of assay protocols, quick buttons for immediate scans and direct saved in text format in order to better address your research data export. Thus offering the highest utility and greatest feature set at a most reasonable price! Simple to use needs. Wavelength selection from 200 nm to 1,000 nm in 1 nm increments is simple and effortless, with the scanning monochromator there is no need to change fixed filters. Spectrophotometer performance verification Performance verification plates and Spectra MR detects the absorbances of DNA, RNA and procedures are available to ensure proper The special Spectra MR Verification Plate containing NIST- documentation and performance to proteins without the need to further modify samples to traceable standards, and IQ/OQ/PQ procedures are specifications of your Spectra MR. obtain correct results. Accurate absorbance additional tools to ensure proper documentation and correct measurements are obtained with the analyzer’s high operation and performance of your analyzer. Critical optical resolution of 2 nm, important for discriminating the performance parameters of the Spectra MR (photometric 260 nm narrow peaks of DNA. Enzyme protocol accuracy, wavelength accuracy, stray radiation and plate Global support designed for confidence conditions are critically maintained in the temperature carrier alignment) are easily monitored and documented DYNEX Technologies is an ISO 9001 certified international company with controlled reading chamber between ambient and 45ºC. within the Revelation application. long-established customers worldwide. It continues to be our privilege to The Verification Plate ensures optimum provide our customers the quality instruments and services they have performance in both the visual and UV come to expect over the past 30 years. We build products and customer wavelengths along with correct plate relationships with confidence, to the highest standards and quality in all alignment. Check instrument accuracy, phases of our operations and customer experience. precision and stray light as well.