TCA cycle

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Shared by: james pausal
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• C H A P T E R • 12 • TCA CYCLE • • • • • • • • • • • • • to fat to fat pyruvate to glucose oxaloacetate isocitrate NADH malate dehydrogenase isocitrate dehydrogenase citrate synthase AcCoA citrate aconitase malate fumarase NADH α-ketoglutarate CoA fumarate succinate dehydrogenase α-KG dehydrogenase succinyl CoA succinate thiokinase NADH FADH2 succinate GTP Figure 12-1 The Tricarboxylic Acid (TCA) Cycle 153 • 154 • Basic Concepts in Biochemistry TCA CYCLE1 (see Fig. 12-1.) To burn the acetyl-CoA made from fat, glucose, or protein in order to make ATP in cooperation with oxidative phosphorylation. Location: All cells with mitochondria. Connections: From glycolysis through acetyl-CoA. Pyruvate makes oxaloacetate and malate through the anaplerotic reactions. To b oxidation through acetyl-CoA. To amino acid degradation through acetyl-CoA and various intermediates of the cycle. Regulation: Supply and demand of TCA cycle. Availability of NAD and FAD as substrates. Inhibition by NADH. High-energy signals turn off. Low-energy signals turn on. ATP yield: Pyruvate ¡ 15ATP Acetyl-CoA ¡ 12ATP Equations: Pyruvate Acetyl-CoA GDP GDP Pi 3NAD 3CO2 GTP Pi 2CO2 2NAD GTP FAD ¡ 3NADH FADH2 FAD ¡ 2NADH FADH2 3H 2H Function: 1 The tricarboxylic acid cycle is also known as the Krebs cycle or the citric acid cycle. Why give something so central to life only one name?

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