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Principles and Applications of Chemiluminescence - Excel

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					                                                                           TOPIC                                        www.chromedia.org
    GC                    Sheet 1 of 4                                  CIRCLES are
                                                                          YELLOW                                         Table of contents
    Cells link to chapters                        Click cell, then arrow, to go to chapter

                         Injection modes,
                                                   Large Volume                                                                                                     Sample Prep                            Troubleshooting
GC fundamentals             Principles &                                  Detection            Detectors                GC-MS                  Columns                                      SPME
                                                      Injection                                                                                                      & Sampling                                  GC
                             Hardware
                                                                                                                  Capillary GC Coupled
                        Injection principles of     Large volume         GC detection        Flame Ionisation                           Packed and capillary The GC Sample                 SPME in         Systematic problem
    Introduction                                                                                                 with Mass Spectrometry
                             capillary GC              injection          principles:          detector (FID)                                columns         Preparation Circle           perspective            solving
                                                                                                                         (GC-MS)

                                                                        > Universal vs.      Problems when          Basics of mass                                  How to select a      Principles of      Rules of thumb &
 GC basics course        Injection techniques         Introduction                                                                        The stationary phase
                                                                           selective          lighting an FID        spectrometry                                      method               SPME                checklist
                                                                                                                                                                                         Experimental
                                                                                                                                                                                          parameters
   Video sessions          Manual syringe          On-column large      > Destructive vs.    Electron Capture                             Column choice and         ST in analytical
                                                                                                                  Ionization techniques                                                    affecting         The gas system
 'Introduction to GC'         injection            volume injection      non-destructive      Detector (ECD)                                 optimization             chemistry
                                                                                                                                                                                           extraction
                                                                                                                                                                                           efficiency
                                                                                                  Basic                                                              Introduction to
    Video preview                                 Techniques for PTV-   > Differential vs.                                                                                              Interfacing and
                        Comparison of injectors                                              catecholamines          Mass analyzers         Fast GC columns              Sample                                  Injection
 'Introduction to GC'                                     LVI                integral                                                                                                      automating
                                                                                                with ECD                                                             Treatment (ST)
                                                                        > Mass-flow vs.         Nitrogen                                                            Sample handling:       Method
    Separation                                                                                                       Mass spectra         High Temp System
                              The septum             Optimisation        concentration-        Phosphorus                                                              a rational      development and         The column
 parameters in GC                                                                                                    interpretation          Conditioning
                                                                           sensitive            Detector                                                               approach          optimization
                                                                                                 Flame                                                                                  Optimization of
    Plate height                                   LVI using speed        GC detector                                 Overview MS
                                 Liners                                                        Photometric                                                                                desorption           The detector
     equations                                    controlled sampling    characteristics:                            instrumentation
                                                                                                Detector                                                                                  conditions
                                                     Programmed
                                                                                            Thermal
Peak width and peak                                  Temperature                                                                                                       Passive          Selection of the  Problems when lighting
                            Direct injection                          > Operating range   conductivity                                      PLOT columns
    broadening                                    Vaporization (PTV)                                                                                                   Sampling        calibration method        an FID
                                                                                            detector
                                                       injection
     Parameters                                   Techniques for PTV-                   Chemiluminescen                                   Porous Layer Open                                                 An overview from
                             Split injection                            > Linear range                              GC-GC GCxGC                                       Introduction     Method evaluation
influencing retention                                     LVI                             ce Detector                                      Tubular columns                                                      practice
                           Installation of a
 The Van Deemter                                                        > Response and       Atomic emission                              Chemically bonded                                                  Some 'bad' GC
                         capillary column to a     Thermodesorption                                               Multi Dimensional GC                                Air Sampling        References
     equation                                                              sensitivity           detector                                  porous polymers                                                   chromatograms
                              split injector
                                                                                                                                                                                           Recently
                                                                                                                  Comprehensive Two-                                                    published food
Video Master Class:                                                     > Selectivity and    Photo Ionisation                                                                                               Troubleshooting in
                           Splitless injection                                                                      dimensional GC                                   Liquid sampling     and fragrance
  Resolution in GC                                                         specificity          Detector                                                                                                         GCxGC
                                                                                                                       (GCxGC)                                                         studies involving
                                                                                                                                                                                             SPME
    Video: GC
                           Flow control and                                                  Helium Ionisation                                                                                                Problems with
   Temperature                                    Headspace analysis    > Detection limit                            Why GCxGC?           Wide Bore Columns
                          pressure regulation                                                     Detector                                                                                                  pesticides analysis
   Programming
                                                                                             Hall Electrolytic
Qualitative analysis:      Cold trapping or       Dynamic headspace      > Background                              Troubleshooting in
                                                                                              Conductivity                                Wide bore columns
   identification         thermal focussing        & purge-and-trap          signal                                     GCxGC
                                                                                                 Detector
 Rules of thumb &                                                       > Noise, spikes,                          Glossary of GCxGC        Wide-bore column
                             Solvent effec
     checklist                                                            wander, drift                                  terms              characteristics
  Some 'bad' GC            Cold on-column                                                                        Download GCxGC book      Wide-bore vs other
                                                    The GC system
  chromatograms                injection                                                                                 here                  columns
                                                                                                                                           Comparison wide-
  How to select a                                                                                                  Introduction to Jan     bore with packed
                          The retention gap       The gas flow system
     method                                                                                                            Beens' book         and narrow-bore
                                                                                                                                           capillary columns

                                                   Flow control and                                                                       Installation of a wide-
       Q&A                                                                                                                                                                                                 Definitions Glossary
                                                  pressure regulation                                                                          bore column

                                                    Gases for GC,
                                                     parameters

                                                    The gas system


                                                     The GC oven
                                                                                               TOPIC                               www.chromedia.org
            LC                          Sheet 2 of 4                                        CIRCLES are
                                                                                                BLUE                                Table of contents
           Cells link to chapters                                Clicking a cell takes you straight to the chapter
                                                                                           2-Dimensional                                                  Separation             Reversed                      Chiral                   Troubleshooting
      LC fundamentals                  The LC-system                 Detection                                          Gradient Elution
                                                                                                LC                                                          Modes               Phase (RPLC)               Chromatography                      LC

                                        The HPLC system,         LC Detectors, general                                                                                                                     STEREOSELECTIVE              Troubleshooting LC,
         LC basics course                                                                  Multidimensional LC               Topic Outline             LC Separation modes          Introduction              ANALYSIS &
                                             basics                    aspects                                                                                                                                                                 basics
                                                                                                                                                                                                           CHROMATOGRAPHY
                                                                    UV absorption                                                                      Guidelines for method                                   Principles of
         Introduction to LC                 LC pumps                                         Heart-cut: LC-LC       Introduction to Gradient Elution                             Stationary phases                                      How to avoid trouble
                                                                      detectors                                                                                choice                                        stereochemistry

                                         Solvent system            Refractive index        Two-dimensional LC                                                                  Physical & Chemical        PRINCIPLES of CHIRAL
   Role of separation techniques                                                                                     Advantages & disadvantages                                                                                         Top tips from practice
                                           essentials            detector (RI detector)         (LCxLC)                                                                             properties                SEPARATION

                                                                                                                                                          Size Exclusion           Retention and           Indirect LC enantiomer       Systematic problem
        Separation process             Fittings, nut, ferrules   Conductivity detector     Combining LC modes             Gradient systems
                                                                                                                                                         Chromatography             Selectivity                   separation                  solving

                                                                                                Optimizing                                                Size Exclusion    Chemical and thermal            Direct LC enantiomer
      Separation parameters               Injection valves       Fluorescence detector                                   Setting up a gradient                                                                                           Pressure problems
                                                                                          experimental conditions                                      Chromatography (SEC)       stability                       separation

                                       Malfunctions, wear,         Detection in ion            Examples of                                                                      Reproducibility and
      The resolution equation                                                                                        Video Class: Gradient Elution                                                         General elution modes           Peak problems
                                         salt formation          chromatography (IC)           applications                                                                        repeatability

                                                                                                                                                                                 Hybrid stationary
Video Master Class: Resolution in LC                                                                                   Gradient troubleshooting          Normal Phase LC                                                                Resolution problems
                                                                                                                                                                                      phases
                                                                                                                                                                                                         General elution modes
                                       Columns & Column                                       NanoLC and                                                                           Mobile phase           1. Coated Polysaccharide-
    The Van Deemter equation                                     Mass Spectrometry                                   Transfer of gradient methods        NPLC / LSC / SPC                                                               Bad column stability
                                           selection                                          applications                                                                          composition                  based CSPs

Effect of separation parameters on     Introduction to HPLC         Basics of mass                                                                       Some application      How to select the right         2. Immobilized
                                                                                                 Nano LC               Training tool for transfer                                                                                        Baseline problems
             resolution                       columns                spectrometry                                                                           examples                  column                polysaccharide CSPs

 Effect of changing parameters on                                                                                    Gradient transfer calculation                               Column ranking &        > Solvent compatibilities of
                                         Column packings         Ionization techniques     The Nano LC system                                                                                                                                The pump
            chromatogram                                                                                                          tool                                           selection guidance                1 and 2

                                                                                                                                                                               Column selection for
                                       Influence of column                                Nano LC in proteomic
     Chromatogram generator                                         Mass analyzers                                  Theory 1: The gradient equation                                pharma and                  3. Protein CSPs                Injection
                                            parameters                                          analysis
                                                                                                                                                                               compendial analysis

                                           Selecting and             Mass spectra
     Separation & peak overlap                                                                                         Theory 2: Peak capacity         Ion Chromatography                                4. Synthetic polymer CSPs           The column
                                       optimizing the column         interpretation

                                        Effect of changing
                                                                                                                                                                                  RPLC column            5. Cyclodextrin(CD)-based
  Peak width and peak broadening         parameters on                   APPI                   Fast HPLC                                                  Topic Outline                                                                    Mobile phase
                                                                                                                                                                                    selection                      CSPs
                                          chromatogram

                                       Column dimensions             Overview MS          Very High Pressures in       Temperature effects &              Ion Exchange             Selecting and          6. Macrocyclic antibiotics
        Test your know-how                                                                                                                                                                                                                    Detection
                                       and peak broadening          instrumentation                 LC                    Programming                  Chromatography (IEC)    optimizing the column               CSPs


                                                                   LC – MALDI MS                                                                         Introduction to ion   How to select the right       7. Donor-acceptor            Diagnosing 'bad'
  Diagnosing 'bad' chromatograms                                                                                       Influence of temperature
                                                                      Coupling                                                                            chromatography              column              (Brush/Pirkle-type) CSPs        chromatograms


                                                                     Speciation                                         High temperature and    Criteria for separation          Column ranking &                                           More 'bad' LC
        How to avoid trouble             Preparative LC                                                                                                                                                  8. Chiral crown-ether CSPs
                                                                    Spectroscopy                                    temperature programmed HPLC      and detection               selection guidance                                        chromatograms

                                                                                                                                                                               Column selection for
                                                                                                                                                          Detection in ion                                                                How to use and
  Before you call the service dept!        Topic outline          Speciation analysis              FFF                   Method development                                        pharma and            9. Chiral anion-exchangers
                                                                                                                                                        chromatography (IC)                                                             regenerate columns
                                                                                                                                                                               compendial analysis

                                                                                                                                                         Ion Suppression                                 10.Chiral ligand-exchange       Bad retention time
    Qualitative analysis, basics                                                          FFF basics short course            Applications
                                                                                                                                                       Chromatography(ISC)                                           CSPs                     stability

                                                                                                                                                            Ion Pair                                     Column selection & method
      How to select a method                                                                                        Advantages and limits of HTLC
                                                                                                                                                       Chromatography (IPC)                                        devel.

                                                                                                                                                                                                           Analysis of enantiomer
           Abstracts LC
                                                                                                                                                                                                                  composition
                                                                                                                                                                                                            Enantiomeric impurity
                                                                                                                                                                                                                 determination
                                                                                                                                                                                                          Links to important column        Definitions &
                                                                                                                                                                                                                   suppliers                 Glossary
         CE                       SFC                  Sheet 3 of 4



      Cells link to chapters                          Click cell, then arrow, to go to chapter
 Basic concepts, theory             SFC

Electroforetic separation
                                 SFC basics
       techniques

    Capillary Zone            Selecting the SFC
    Electrophoresis              parameters

                               General Column
                                 parameters

                            Temperature and outlet
                                  pressure

                            Modifiers (Co-solvents)
                      This is the table of contents of
                            www.chromedia.org



ell, then arrow, to go to chapter
   GENERAL                                                                                                                 www.chromedia.org
                                        Sheet 4 of 4
    TOPICS                                                                                                                  Table of contents
            Cells link to chapters                                Click cell, then arrow, to go to chapter
        Other Topics                  Troubleshooting GC          Troubleshooting LC           Sample Treatment                           SPME                            Pesticide Analysis                       Pharmaceutical Analysis                   Synthetic Polymers

There is more than the analytical                                  Troubleshooting LC,                                                                                                                    Research and Development in the Pharmaceutical
                                     Systematic problem solving                                Outline of the full topic            SPME in perspective               Pesticide determination by LC                                                            Analysis of polymers
         technique only                                                   basics                                                                                                                                             Industry

      Inside the black box           Rules of thumb & checklist    How to avoid trouble        How to select a method               Principles of SPME              The analysis of pesticide residues                 Separation techniques                   Polymer distributions

                                                                                                                            Experimental parameters affecting
     From sample to result                The gas system           Top tips from practice     ST in analytical chemistry                                                   LCMS of pesticides                     > LC in pharmaceutical analysis             Chemical composition
                                                                                                                                   extraction efficiency

                                                                   Systematic problem          Introduction to Sample                                                   Interfacing systems, ESI,                                                              Detailed Molecular
The test method is lab's treasure             Injection                                                                          Interfacing and automating                                                              > Other LC modes
                                                                         solving                    Treatment (ST)                                                              APCI,APPI                                                                       Characterization

                                                                                             Sample handling: a rational         Method development and
              LIMS                          The column              Pressure problems                                                                                        Mass analyzers                      Quantitative & Qualitative analysis          Measuring conversion
                                                                                                     approach                          optimization

                                            The detector              Peak problems           Metabolism and stability      Optimization of desorption conditions                                                       Sample preparation                    Sampling and sample
                                                                                                                                                                                                                                                                     handling
Measuring & validating results       Problems when lighting an     Resolution problems                 Quality               Selection of the calibration method             Food Analysis                           Validation of LC methods                   List of acronyms
                                               FID
         Quantification              An overview from practice     Bad column stability              Validation                      Method evaluation                         Introduction                            Recent developments                         References

                                          Some 'bad' GC             Bad retention time                                                                                 Food and fragrance studies
           Calibration                                                                      Sampling of gases and liquids               References                                                                 Release and stability analysis
                                          chromatograms                  stability                                                                                          involving SPME

                                                                                                                                Recently published food and
       Statistical Testing           Troubleshooting in GCxGC       Baseline problems            Sampling of solids                                                           Nutrigenomics                                                                          Courses
                                                                                                                             fragrance studies involving SPME

    Linear Calibration curve          Problems with pesticides          The pump                                                                                              PAHs in food                                Water Analysis                        GC basics course
                                             analysis
           Validation                                                    Injection               Passive sampling                          LPME                               PAH analysis                                    Outline                            LC basics course

         Quality Control                                                The column                  Introduction              Principles of Hollow Fiber LPME            Biogenic amines in wine                      Issues in water analysis          LC troubleshooting advanced course

                                                                       Mobile phase                 Air Sampling                  HF/LPME in perspective            Analysis of biogenic amines in wine             Water analysis, introduction

     Method Development                                                  Detection                 Liquid sampling              Preparation of hollow fibers                                                      Analysis of inorganic compounds
                                                                     Diagnosing 'bad'                                            Method development and
          Introduction                                                                                                                                                         Proteomics                      Steps in analysis of organic compounds
                                                                     chromatograms                                                     optimization
                                                                       More 'bad' LC
    Classification of methods                                                                                                      Applications overview                       Introduction                                Some trends
                                                                      chromatograms
                                                                     How to use and
     When to use methods?                                                                                                                                              Peptide mass fingerprinting                        Further reading
                                                                   regenerate columns
 Strategy for selective sample
                                                                                                                                                                     2D gel electrophoresis (2-DGE)
    treatment and detection.

    How to select a method

    Chromatogram generator

Gradient transfer calculation tool

 Clever choices for optimization

				
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Description: Principles and Applications of Chemiluminescence document sample