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									Allahabad Agricultural Institute-Deemed University




     APPENDIX -XXII




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                                                        APPENDIX – XXII
                                  Course Structure
                          B. TECH. BIOTECHNOLOGY (GE/BT/MT)
                                          SEMESTER-I
Course Code                            Course Title              Credits      L-T-P
CHEM-310      ORGANIC CHEMISTRY                                     3          2-0-2
ME-310        ENGINEERING WORKSHOP                                   2         0-0-4
PHY-312       ENGINEERING PHYSICS                                    5         3-1-2
GPT-301       MORAL AND VALUE EDUCATION                              3         3-0-0
BIOL-403      LIFE SCIENCE I                                         2         1-0-2
CHEM-311      INORGANIC CHEMISTRY                                    3         2-0-2
MAS-311       ELEMENTARY MATHEMATICS                                 2         2-0-0
BIOL-201      ELEMENTARY BIOLOGY                                     2         2-0-0
SES-415       ENVIRONMENTAL STUDIES-I                                2         1-0-2


                                          SEMESTER-II
Course Code   Course Title                                       Credits     L-T-P
ME-301        ENGINEERING GRAPHICS                                  3        0-0-6
PHY-308       BIOPHYSICS                                             3        3-0-0
BCBT 428      BIOCHEMISTRY I                                         3        2-0-2
CHEM -330     ANALYTICAL CHEMISTRY                                   3        2-0-2
BIOL-404      LIFE SCIENCE-II                                        2        1-0-2
COMP-410      COMPUTER AND LANGUAGES                                 3        2-0-2
MBGE-327      INTRODUCTORY BIOTECHNOLOGY                             3        2-0-2
MAS-411       ENGINEERING MATHEMATICS-I                              3        3-0-0
SES-416       ENVIRONMENTAL STUDIES-II                               2        1-0-2

                                         SEMESTER-III
Course Code   Course Title                                       Credits     L-T-P
MBMT-351      MICROBIOLOGY-I                                        3        2-0-2
MBGE 326      MOLECULAR BIOLOGY                                      3        2-0-2
CHEM-312      PHYSICAL CHEMISTRY                                     3        2-0-2
MAS-490       ENGINEERING MATHEMATICS-II                             3        3-0-0
CE-379        FLUID MECHANICS & TRANSPORT PROCESS                    3        3-0-0
BCBT430       BIOCHEMISTRY II                                        3        3-0-0
MAS-511       STATISTICAL METHOD                                     3        2-0-2

                                         SEMESTER-IV
Course Code   Course Title                                       Credits     L-T-P
EEE-301       ELECTRICAL ENGINEERING                                4        2-1-0
GPB- 412      GENETICS                                               3        2-0-2
MBMT-401      MICROBIOLOGY II                                        3        2-0-2
COMP-302      INFORMATION TECHNOLOGY                                 3        2-0-2
ME-419        PRINCIPLES HEAT & MASS TRANSFER                        3        3-0-0
BCBT-451      ENZYMOLOGY                                             3        2-0-2
BCBT-440      CHEMICAL THERMODYNAMICS                                3        3-0-0
CBAS-400      TRAINING-I                                            1         0-0-2
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                                          SEMESTER-V
Course Code   Course Title                                         Credits     L-T-P
ECE-430       ELECTRONIC MEASUREMENT & INSTRUMENTATION                3        2-0-2
BCBT-452      BASIC IMMUNOLOGY                                         3        2-0-2
MBGE-441      MOLECULAR GENETICS                                       3        2-0-2
MBGE-451      PLANT BIOTECHNOLOGY                                      3        2-0-2
MBGE-452      ENVIRONMENTAL BIOTECHNOLOGY                              2        2-0-0
BCBT-452      CHEMICAL ENGINEERING                                     3        3-0-0
BI-490        BIOINFORMATICS                                           3        2-0-2
MBGE-454      MOLECULAR BIOLOGY TECHNIQUES &                           3        2-0-2
              INSTRUMENTATION
LNG-304       PROFESSIONAL COMMUNICATION AND TECHNICAL                 3        3-0-0
              WRITING

                                         SEMESTER-VI
Course Code   Course Title                                         Credits     L-T-P
MBGE-501      BIOSAFETY, BIOETHICS & IPR ISSUES                       3        3-0-0
BAM-502       MARKETING & MANAGEMENT OF BIOTECHNOLOGY                  3        3-0-0
              PRODUCTS
TC-502        TISSUE CULTURE                                           3        2-0-2
BCBT-501      BIOCHEMICAL ENGINEERING                                  3        2-0-2
MBGE-502      RECOMBINANT DNA TECHNOLOGY (GE)                          3        2-0-2
BCBT-502      INDUSTRIAL BIOTECHNOLOGY (BT)                            3        2-0-2
MBMT-501      MICROBIAL BIOTECHNOLOGY                                  3        2-0-2
MBGE-453      ANIMAL BIOTECHNOLOGY                                     3        2-0-2
BAS-488       SEMINAR-I                                                1        0-0-2
BAS-500       TRAINING-II                                              1        0-0-2


                                         SEMESTER-VII
                                GENETIC ENGINEERING (GE)
Course Code   Course Title                                         Credits     L-T-P
MBGE-601      MOLECULAR BREEDING                                      3        2-0-2
MBGE-602      GENOMICS                                                 3        2-0-2
MBGE-605      BIOMOLECULAR MODELING                                    2        1-0-2
MBGE-603      GENETICALLY MODIFIED FOODS                               3        3-0-0
MBGE-604      GENE CLONING & GENE THERAPY                              3        2-0-2
BAS-589       SEMINAR-II                                               1        0-0-2


                              MICROBIAL TECHNOLOGY (MT)
Course Code   Course Title                                         Credits     L-T-P
MBMT-601      FERMENTATION TECHNOLOGY                                 3        2-0-2
MBMT-602      MICROBIAL PROCESS ENGINEERING                            3        2-0-2
MBMT-603      CLINICAL BIOTECHNOLOGY                                   2        2-0-0
MBMT-604      MICROBIAL ENZYME TECHNOLOGY                              2        2-0-0
MBMT-605      MICROBIAL TECHNOLOGY IN FOOD INDUSTRY                    2        2-0-0
BAS-589       SEMINAR-II                                               1        0-0-2

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                             BIOCHEMICAL TECHNOLOGY (BT)
Course Code   Course Title                                        Credits      L-T-P
BCBT-601      BIOPROCESS ENGINEERING & DOWN STREAM                    3        2-0-2
              PROCESSING
BCBT-602      IMMUNOLOGY, VACCINE & TRANSPLANTATION                    3        2-0-2
              TECHNOLOGY
BCBT-603      MEDICAL & MOLECULAR DIAGNOSTICS                          2        1-0-2
BCBT-604      PHARMACEUTICAL BIOTECHNOLOGY                             2        2-0-0
BCBT-605      BIOPOLYMER TECHNOLOGY                                    2        2-0-0
BAS-589       SEMINAR-II                                               1        0-0-2


                                        SEMESTER VIII
Course Code   Course Title                                        Credits      L-T-P
BAS-699       Project (GE/BT/MT)                                      9        0-0-18




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                                                   SEMESTER I


 CHEM-310                                       Organic Chemistry                                          3(2-0-2)
UNIT I: chemistry of carbohydrates
UNIT II: amino acid and protein
UNIT III: Benzene and its homologous, structure of benzene, aroma, nomenclature and aromatic compounds
UNITIV: General introduction of alkaloids
UNITV: General introduction of terpenes
UNIT VI: introduction to analytical chemistry and its branches
UNITVII: General principles of analytical chemistry, volumetric analysis, concept of gram equivalents and
method of expressing concentration of solution, preparation of primary and secondary standard solutions.
Neutralization (acid –base), titration permagnometry, gravimetric analysis: principles of methodology
estimate of calcium
UNIT VIII thermometric analysis the gravimetric analysis, types, instrumentation, application
UNITIX Polarimetry
UNIT X Potentiometric titration, electrode system, ion selective membrane electrodes, application of
potentiometric titrations
PRACTICALS
Qualitative analysis of organic compounds
Volumetric determination of Fe by KMnO4 solution
Polarimetric analysis
Estimation of protein by kjeldal method
Estimation of calcium volumetrically.



 ME-310                                      Engineering Workshop                                          2(0-0-4)
Introduction to engineering materials and its classification, metals and its classification – ferrous and non-
ferrous metals and its alloys, cast iron steel and alloy steel
Brief introduction – description and uses of hand tools used in carpentry shop, fitting shop, sheet metal work,
smithy shop, plumbing shop, welding shop.
Welding –introduction to welding and its classification, gas welding, electric welding, soldering and brasing
Various types of fasteners and fastening devices, threads, terminology of threads, various forms of threads.
Properties of engineering materials- mechanical properties – strength
elasticity, stiffness, plasticity, malleability, ductility, brittleness, toughness, hardness, impact strength , fatigue
and creep
Practical
    1. Carpentry shop, - simple job on vertical and horizontal sawing , planning and slot making in wood ,
        (ii) tomake t- lap joint
    2. Smithy shop – to make o- ring from a given rod (ii) to prepare s- hook
    3. Fitting shop –(i) simple exercise on filing, v- groove and vertyical sawing (ii) to make square from
        given piece of mild steel by filing and hack sawing
    4. Welding shop –(i) to makle butt weld of two given mild steel pieces (ii) to make lab weld of two
        given pieces (iii) soldering and brazing practices
    5. Sheet b metal shop –(i) to make book stand from given piece of sheet metal of 18 SWG
    6. Introduction of lathi machine showing various parts.




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 PHY-312                                       Engineering Physics                                           5(3-1-2)
Units and measurements, dimensions and dimensional analysis
Speed ,velocity,acceleration ,momentum ,impulse,torque , kinematics in one , two and three dimensions .
Newtons law of motion, conservation of energy and linear momentum, weight and mass of body ,Newtons
law of universal gravitation , satellite and weightlessness, keplers law of planetary motion , types of force in
nature .
Equilibrium and elasticity, stress, strain, hookes law elastic limit
Machines and their principle, efficiency, mechanical advantage, velocity ratio, relationship between
efficiency, mechanical advantage and velocity ratio , levers and their kinds .
Pressure in fluids and their laws, Pascals law, atmospheric pressure, gauge press, measurement of press,
simple barometer, buoyancy and Archimedes principle, principle of floatation and it has applications.
hydrometer and lactometer . Surface tension, capillary, equation of continuity, bernoullis equation, viscosity.
Thermometry, relationship between the scales of temperature, absolute scale of temperature and clinical
thermometer, specific heat, latent, conduction, conviction, radiation
Rectilinear propagation and reflection of light, formation of image of a point object by a plane mirrors,
colours
Sound, pitch, frequency, wavelength, velocity of sound echoes
Electricity, simple cell, relation ship between current charge, potential, oms law resistance, series and parellel
combination
Electrical power, watt and KWH, household electricity.


 GPT-301                                   Moral and Value Education                                         3(3-0-0)
My country and my people, the many Indians, being and becoming Indian, nationalism and internationalism
Some life issues – love, sex and marriage, men and money – value of time, meaning of work, human
communication, human suffering, addition, ecology, women issues
Understanding ones neighbor, neighborhood groups: their structure and their functions patterns of social
interactions of group dynamics. Preparation for a carreer, choice of vocation, motivation of study and
research, and the present educational system: curricu8lam and syllabus, teaching methods, examination and
work experience
Definition of value education, moral and ethics, laws and morale based on ten commandments and two great
commandments
Discovery of self, self – awareness, growth of intellect – mans spiritual life emotions, will respect the rights
of life, liberty, property, truth reputation
Sin , origin of sin , manifestation of sin , the result of sin , the remedy of sin , sin as an act , sin as a state , sin
as a nature .


 BIOL- 403                                           Life Science-I                                          2(1-0-2)
1. Origin of Life: History of earth, theories of origin of life nature of the earliest organism.
2. Varieties of Life: Classification, Five kingdoms, viruses(TMV, HIV, bacteriophage),
    Prokaryotes(Bacteria-cell structure, nutrition,reproduction), Proyista,fungi,Plantae and animalia.
 3. Chemicals of life: (Biomolecules)-Carbohydrates, lipids amino acids, proteins, nucleic acids,
    identification of biomolecules in tissues.
4. Histology: Meristems(apical, intercalary, lateral) and their function;simple issues(parenchyma,
    collenchyma,         sclerenchyma);          Complex          tissue(xylem          and      phloem);Tissue
    systems(epidermal,ground,vascular);primary body and growth(root,stem and leaf); secondary growth.
    Animal Epithelial tissue, connective tissue, muscle tissue and nervous tissue and their function in body.




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5. Nutrition: Autotophic(photosynthesis) Pigment systems, Chloroplast, light absorption by chlorophyll
   and transfer of energy, two pigment systems, photosynthetic unit, phosphorylation and electron transfer
   system,Calvin-Benson Cycle(C3), Hatch Slack Pathway(C4), Crassulacan Acid Metabolism(CAM),
   factors affecting photosynthesis;Mineral nutrition in plants.
   Heterotrophic-Forms of heterotrophic nutrition, elementary canal in Humans,          nervous and hormonal
   control of digestive systems, fate of absorbed food       materials;Nutrition in humans,Referance values.
6. Energy Utilization: (Respiration) –Structure of mitochondria, cellular respiration, relationship of
   carbohydrate metabolism to other compounds, Glycolysis, fermentation, formation of acrtyl co-A,Kreb
   cycle, Electron Transport System and Oxidative Phosphorylation, ATP,factors affecting respiration.
7. Transport : Plant water relationship Properties of watwer diffusion, osmosis,imbibition movement of
   water in flowering plants, uptake of water by roots,ascent of water in xylem. Apoplast , symplast theory,
   transpiration structure of leal and stomata in plants opening and closing mechanism of stomata factors
   affecting transpiration, significance of transpiration, general characteristics of         blood vascular
   system,development of blood systems in animals, composition of blood, circulation in blood
   vessel,formation of tissue fluids, the heart,function of mammalian blood, the immune system


 CHEM 311                                    Inorganic Chemistry                                        3(2-0-2)
Periodic table: Mendleef periodic table, modern periodic table and table. Long and extended form of periodic
table, its main features, merits and demerits.
Pereidic properties:
Atomic and ionic radii: classification of atomic radii (1) covalent radii (2) metallic radii (3) vander-waal radii,
ionic radii.classification of ionic radii (1) size of anions, size of cations, crystal coordination number, radius
ratio, size of anions, factors influenzing magnitude of ionic radii. Periodic variation of atomic and ionic radii.
Ionization energy: introduction, successive ionization energies. Factors influenzing ionization energy (nuclear
charge, size of atom, ion. shielding effective nuclear charge, principal quantum number, half and full filled
orbitals) trend of ionization energy in the periodic table
Electronic affinity: first and second electron affinity. Factors effecting magnitute of electron affinity. Periodic
variation
 Radioactivity: introduction of radioactivity, radioactive emmision ( alpla ,beta and gamma rays ). Half-life
period, average life period, radioactive equilibrium. Group displacement law, atomatic and induced
radioactivity.
Nuclear chemistry: nuclear fussion and nuclear fission reactions.
Mass defect, binding energy.


 BIOL-201                                     Elementary Biology                                        2(2-0-0)
LIFE: Living and non-living
Origin of life:oparin abiotic theory
Evolution: unicellularity, multicellularity, complex tissue system
Introduction to various systems of biology
Introduction to botany: history of botany, brief intriduction of branches of botany , morphology, anatomy,
taxonomy , physiology, paleobotany
Introduction to lower botany: Algae, fungi, lichen, bacteria, virus, bryophytes, pteridophytes,
gymnosperms.
Introduction to zoology:classification of animal kingdom, adaptation of animals. external morphology of
frog, internal anatomy of frog – internal organs , different internal systems .
Scope : application of biology




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 BSH 303                                 Elementary Mathematics                                     3(3-0-3)

ALGEBRA – theory of quadratic equations (a2+b+c)=0. Binomial theorem, uses of natural and common
logarithms, exponential series, partial fractions, determinants (of order 3 only), theory of matrixes (additon,
substraction), product of matrices, transpose, elementary idea of: adjoint, inverse. Solution of linear
equations, solution of inequalities, permutations and combinations.
Trigonometry: Trigonometric functions, addition and substraction formula, double and half angle formula,
laws of sines and cosines, solutions of triangles, height and distances, real complex numbers, hyperbolic
trigonometric functions, de moivers theorem
Coordinate geometry: Distance between two points, area of triangles, straight lines, (parallel and
perpendiculars).

 SES-504                                     Environmental Studies                                    3(2-0-2)

Defining Environment, atmosphere model: Troposphere, stratosphere, mesosphere, thermosphere,
comparison of clear and dry air
Hydrological cycle, components of hydrologic cycle, role of water in pollutant cycle in soils
Pollutants: Definition and classification, air, water and soil pollution
Soil and water pollution due to NO3, phosphates and heavy metals, NO3 pollution due to organic matter
decomposition, mineralization, nitrogen cycle, negative environmental effects of NO3, PO4-3 and other metal
ions on human health; phosphate loading to surface water bodies through characteristic overland flow,
sediment delivery ratio, runoff ratio/coefficient, enrichment ratio, water quality indeed, water quality
standard, sewage treatment, water purification and management water quality index, water quality standard,
sewage treatment, water purification and management strategies
Air pollution: concept, air pollution problem in India, present status
Type and sources of urban and industrial pollutant
Responses of crops to important phytotoxic air pollutants (SO, NO, Ozone, HF etc)
Acid rain: causes and consequences with special references to crops. Nuclear pollution and consequences. Air
pollution control and alternate strategies. Risk assessment and warnings
Practical:
Determination of pH; Electrical conductivity; Na, K, Ca, Li (flame photometer method); acidity and
alkalinity; organic carbon content, chloride, carbonate, bicarbonate and sodium adsorption ratio of soil and
water samples




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                                                SEMESTER - II
 ME-301                                      Engineering Graphics                                       3(0-0-6)
Introduction to engineering drawing, about the drawing instruments
Dimensional, lettering, scales
Drawing of various geometrical curves like ellipse, parabola, cycloid.
Concepts of first angle and third angle projection, projection of points, lines, planes,
projection of solids
Orthographic projection of sample objects, missing views, sectorial views
Isometric projection of simple objects – drawing of isometric views from the given
orthographic projections


 PHY-308                                            Biophysics                                          3(3-0-0)
1. Concepts of biophysics and classification, living body as a thermodynamic system, biological effects of
   radiation, radiation hazards in man
2. Biological effects of light, biophysics of vision, interaction of UV light with living system, interaction of
   microwaves in living system
3. Cronobiology, cybernetics, biomagnetism and magneto biology, bioacoustics – the ear
4. Influenze of gravity, g-force, acceleration and decelation, influence of barometric pressure and vibration,
   elementary idea of thermodynamics


 BCBT-428                                    General Biochemistry                                       3(2-0-2)
Chemical structure of proteins and their properties, cellular membrane and their transport phenomenon
Intermediary metabolism: Concept of anabolism and catabolism, metabolism of carbohydrates, lipids and
amino acids and their interrelationship.
Biological oxidation: Electron-transport system, oxidative phosphorylation, free energy changes in
biochemical reactions, energy rich compounds
Metabolism of nucleic acids and proteins
Metabolic regulation of various animal hormones
Practical
Proximate composition of food and biological samples i.e., moisture, fat, proteins, carbohydrates
Determination of constituent of milk protein, fat, lactose.
Determination of ascorbic acid, acidity/ citric acid in fruit and vegetables
Determination of sugar in urine and blood.
Determination of cholesterol in blood and biological sample.


 CHEM-422                          Chemistry-II (Analytical Chemistry)                                  3(2-0-2)
Introduction to Analytical chemistry and its branches.
General principle of analytical chemistry: Volumetric analysis.
Concept of gram equivalents and method of expressing , Concentration of solution
preparation of primary and secondary, Standard solution Neutralized (Acid-Base),
Titration, Permagnometry. Gravimetric analysis.& methodology.
Estimate of calcium.
Thermometric analysis: The gravimetric Analysis – types, Instrumentation, Application.
Polarimetry.
Potentiometer titration: Electrode system, Ion selective membrane Electrodes, Application of
potentiometer titrations


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 BIOL-404                                        Life Science-II                                      2(1-0-2)
Coordination and control: Plant movement(tactic, tropic and Nastic) , Plant growth substances(auxins,
cytokinins, gibberellin, ABA and Etylene) , Phytochrome and effect of light on plant development ,
vernalization and flowering. Nervous system , parts of nervous system, sensory receptors, structure and
function of receptors, endocrine system, role of hormones in growth and development in humans.
Homeostasis: Control system in biology, control of blood glucose level, temperature regulation in
endothermic animals, the liver and its importance.
Reproduction: Asexual reproduction - apomixis and other means of natural vegetative reproduction (bulb,
corm, rhizome, stolon, runner, tuber, tap root ,tillers), advantage and disadvantage of natural asexual
reproduction. Artificial propagation- cutting , grafting budding , layering, micro propagation through tissue
culture ,advantage and disadvantage of micro propagation .
Sexual reproduction-life cycle of flowering plants, the parts of a flower(dicot and monocot),
microsporogenesis, in vitro pollen culture, microgametogenesis, isolation of sperms, palynology, scope of
palynology, development of ovule, types of ovule, megasporogenesis, megagametogenesis, embryo sac, seed
formation, structure of seed and its importance. Review of sexual reproduction in vertebrates, human
intervention in reproduction.
Continuity in Life: Chromosomes, cell cycle, mitosis, meiosis, techniques to study mitosis and meiosis.
Hereditary and Variation: Mendel’s work, chromosomal basis of inheritance, modified dihybrid ratio, gene
interaction, Linkage, gene mapping, sex determination, cytoplasmic inheritance, variation and mutation.
Economical Importance Plants: Cereals (wheat, rice, maize), Beverages (tea, coffee, cocoa), Fibers (jute,
linen, cotton), wood (pines, cedar, teak, sisam), rubber (Para robber), spices (turmeric, black pepper, cloves,
coriander), medicinal plants (Ephedra, Taxus, Cinchona, Fox glove, Belladonna, Ravolfia, neem, Hemp).


 COMP-410                                Computer and Languages                                       3(2-0-2)

Introduction to computers: Computer organization and peripherals. Hardware, software concepts,
terminology. Introduction to DOS – DOS external commands, DOS internal commands. Introduction to
windows- Start menu and accessories, windows explorer, my computer. Introduction to networking of
computers. Program development using BASIC- Algorithms and flow charts, variables, constants, relational
logical operators, library functions, hierarchy rules for operators and evaluations of expressions, LET,
INPUT, READ……. DATA, control statements, GOTO, IF---THEN, FOR---NEXT, Introduction to array and
DIM statement, Introduction to searching, sorting, introduction to functions and subroutines. Steps in
development of applications. Introduction to information management- Data storage, retrieval, data
manipulation, validation and security of data, presentation of data and report generation. Introduction to MS-
Office. Introduction to RDBMS


 BIOT 303                               Introductory Biotechnology                                    2(2-0-0)
Introduction, History of Biotechnology, Branches of Biotechnology and its application in Biotechnology.
Structure, Composition and type of DNA, various types of RNA. DNA as a Genetic material.
Transcription, Translation, Various mechanisms in control of Transcription andTranslation.
Enzyme in Biotechnology, Restriction Enzyme, Ligases, Alkaline Phosphatases.


 BSH-402                                         Mathematics                                          3(3-0-0)
Differential calculus: Functions, limit, continuity and differentiability. Differential coefficient of standard
functions (algebraic, trigonometrically, exponential, logarithmic etc) rules for differentiation, differentiation
of composite, inverse implicit and parametric functions, higher order derivatives, tangent and normal, maxima
and minima
Integral calculus: Indefinite integration of standard functions etc, definite integrals properties, its
applications in evaluating area and volume
Vector algebra: Definition and identification of vectors and scalars, li0near operations in vectors, linear
combination of vectors, linear dependence and independence. Dot and vector and vector products.



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 SES-504                                 Environmental Studies                                   3(2-0-2)
Defining Environment, atmosphere model: Troposphere, stratosphere, mesosphere, thermosphere, comparison
of clear and dry air
Hydrological cycle, components of hydrologic cycle, role of water in pollutant cycle in soils
Pollutants: Definition and classification, air, water and soil pollution
Soil and water pollution due to NO3, phosphates and heavy metals, NO3 pollution due to organic matter
decomposition, mineralization, nitrogen cycle, negative environmental effects of NO3, PO4-3 and other metal
ions on human health; phosphate loading to surface water bodies through characteristic overland flow,
sediment delivery ratio, runoff ratio/coefficient, enrichment ratio, water quality indeed, water quality
standard, sewage treatment, water purification and management water quality index, water quality standard,
sewage treatment, water purification and management strategies
Air pollution: concept, air pollution problem in India, present status
Type and sources of urban and industrial pollutant
Responses of crops to important phytotoxic air pollutants (SO, NO, Ozone, HF etc)
Acid rain: causes and consequences with special references to crops. Nuclear pollution and consequences.
Air pollution control and alternate strategies. Risk assessment and warning




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                                                 Semester III
 MCB-301                                    Microbiology-I                                           3(2-0-2)
History, scope and development of microbiology
Concept of eukaryotic and prokaryotic cells
General characteristics, structure and reproduction of bacteria, actonomycetes , algae, fungi, protozoa ,
mycoplasma , ricketsia , chalamydia and viruses .
Control of microorganisms by physical and chemical agents
Practicals:
Preparation of media and reagents, their sterilization
Culture techniques
Staining techniques (simple, differential, special) in bacteria


 MBGE-326                                     Molecular Biology                                      3(2-0-2)
Structure and properties of nucleic acid: Models of DNA structure, RNA structure, Physical, Chemical,
Stereoscopic Nuclear & organelle genomes,
Genome Complexity: LC value paradox, cot analysis, Repetitive DNA, satellite DNA, pseudo genes,
Synteny.
Chromosome Organization: Histones, Non-histones, Nuleosomes, chromatin, Chromosome structure in
prokaryotes and eukaryotes,
Gene Organization: Split genes, Overlapping genes, Transposons, gene clustuers,
 DNA-Protein interaction: DNA binding motifs, methods of studying DNA binding proteins
 DNA Replication: Models of DNA replication, enzymology of DNA replication, the replication process,
initiation, elongation and termination of replication; telomeres.
Transcription and mRNA processing: Components of transcriptional machinery in prokaryotes and
eukaryotes; initiation, elongation and termination of transcription; capping, polyadenylation, spicing, mRNA
stability
Translation: The genetic code; tRNA and aminoacyl sythetases, ribosomes, Translation process, initiation,
elongation and termination of transcription; Capping, Polyadenylation, Splicing, mRNA stability.
Regulation of Gene Expression: General aspects of regulation in prokaryotes and eukaryotes; the operon
model, lac and trp operons;DNA methylation;Tissue-sp. and developmental stage sp.Expression of genes.
Molecular Evolution: DNA based phylogenetic trees and their applications.
 Gene mutation: Somatic vs germinal mutation, mutant types, selective systems, induction of mutations,
chromosomal mutation, changes in chromosome structure, mutation and cancer, mutagens in genetic
dissection, mutation readings, molecular basis of gene mutations, repair defects and human diseases,
recombination, transposable genetic elements


 CHEM-312                                    Physical Chemistry                                      3(2-0-2)
Ionic equilibria: concepts of acids and their related strength, buffer solution and its ph, hydrolysis of salts,
acids –base indicators oswalds and quinonoides theory, solubility product
Chemical kinetics: order and molecularity, differential rate lawsand integrated rate laws equations for zero,
1st, 2nd and 3rd reactions (derivations included) significance of rate constant and its evaluation, time for
definite fractional change of reaction, determination of order
Electro chemistry: reversible and irreversible cells, EMF of a cell and free energy, nernst equation,
equilibrium constant, standard electrode potential, types of reversible electrodes, applications of
EMFmeasurements, determination of solubility product, ph, dissociation constant of acids, hydrolics constant
solubility, soluble salts
Practicals :
Analysis of inorganic mixture, redox and acid base titration. Preparation and standardization of primary and
secondary standard solution, ph, buffers, chemical kineticsand partition coefficient.



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    Allahabad Agricultural Institute-Deemed University


 MAS-490                                Engineering Mathematics-II                                      3(3-0-0)
Differential calculus: partial differentiation, Euler theorem, total differential coefficient, partial higher order
derivatives, application of partial differentiation approximation problems, error determination, maxima –
minima functions of two variables, Jacobeans
Integral calculus: definite integrals and their properties, their application in determining arc length and
surface area, Simpson rule for approximation, integration, mean values and root mean square values. Multiple
integrals – double and triple integrals, their application in determining area and volume
 Differential equations :Ordinary differential equations, their order degree and formation, solution of the
equations of the first order and first degree. Homogenous and differential equations, linear differential
equations, exact differential equations, linear differential equation of second order with constant equations,
homogenous linear equations.
Vector calculus: vector differentiation, gradient, divergence and curl, their physical interpretation directional
and normal derivatives vector integration, line surface and volume integrals, green theorem, gauss theorem
and stoke s theorem
Fourier series: periodic functions, fouriers series fourier series and their coefficients and their determination
(euler formula) ,change of interval , half range sine and cosine series



 CE-379                           Fluid Mechanics & Transport Process                                   3(3-0-0)
Introduction to principles of engineering and units: classification of unit operation and transport process
and basic system of units, methods of expressing temperature and composition gas law and vapour pressure,
conservation of mass and material balances, energy and heat unit, conservation of heat energy and heat
balances
Principles of momentum transfer: introduction, fluid statics, and viscosity of fluid s, mass energy and
momentum balances, and non-Newtonian fluids
Principles of steady state heat transfer: Introduction and mechanism of heat transfer, conduction,
conduction through solid in series, forced convection heat, transfer in fluids, natural convection heat transfer
and heat transfer of non Newtonian fluids
Principle of mass transfer: Introduction the mass transfer and diffusion, molecular diffusion in gas and
liquid biological solution and cells, mass transfer in cellular systems
Book: Transport process and unit operation by Christie j. geankoplis


 BCBT-430                        Biochemistry-II (General Biochemistry)                                 3(2-0-2)
Nutritional significance: nutrients viz., carbohydrates, fats, proteins, minerals.
Nitrogen metabolism, nitrogen fixation, transformation of nitrogen compounds in biological system,
nitrification and nitrate respiration
Biochemistry of seed germination: carbohydrates, proteins and lipid metabolism during germination and
early seedling growth.
Biochemistry of crop quality: Biochemical changes during storing of seeds, biochemical role of vitamins.


 MAS-511                                       Statistical method                                       3(2-0-2)
Definition and application of statistics, geographical representation of frequency distribution, measures of
central tendency, measures of dispersion, standard error of mean, coefficient of variation
Probability: definition and applications, numerical problems
Simple correlation between two variables (definition, properties and numericals)
Regression lines: definitions and uses, methods of least square, concept of regression coefficient




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                                                SEMESTER IV
 EEE-301                                 Electrical Engineering                                         4(2-1-0)
UNIT I: Electrical circuits and circuit parameters, voltage, current, power energy, basic circuit components –
resistance, inductance, capacitance, ohms law, series and parallel combination of resistance, voltage and
current division in series and parallel circuits, voltage and current sources in series and parallel circuits,
voltage and current sources, source transformation, kirchoffs current law, electrical circuit analysis using
KVL & KCL
 UNITII:       Fundamental of ac circuits: impedance admittance reactance and complex power real reactive
power analysis of circuits using Maxwell’s loop, current method and Maxwell nodal method of voltage,
average, rms, form factor of sinusoidal wave form
UNITIII: network theorems a) thevenins theorem b) Norton theorem c) superposition theorem d) maximum
power transfer theorem
UNIT IV: polyphase system three-phase system line phase line voltage. Phase current, phase voltage of three
phase stare or delta connected systems relation between the current and phase current and line voltage and
phase voltage in three phase star and delta connected system, balanced and unbalanced three phase system.
Three phase electrical power measurement using two-watt meter method
UNIT V: Measurement and measuring instruments. Measurement of electric current voltage power energy
construction and working principles of ammeter voltmeter wattmeter energy meter
Magnetic circuit and transformer b-h curves magneto motive force (MMF) flux reluctance in a magnetic
circuit relation between electric and magnetic circuits, basic principle of a single phase transformer EMF
equation, transformation ratio and equivalent circuit of transformer, open circuit test and short circuit test in a
single phase transformer, losses and efficiency
UNIT VI: electric machines dc machines – construction and working of dc generator, dc series, dc shunt and
dc compound generators and motors, the internal characteristics of dc generators, charecteristic of dc motors,
working and constructions, application of dc motors in field of biotechnology. AC machines – induction
motor, working and construction, equivalent circuit, slip, synchronous speed. Single phase and three phase
induction motor. Synchronous machine – introduction to construction and working, application in field of
biotechnology
UNITVIII: Introduction to power system, power system components, protection of power system, per unit
system, safety device fuse



 GPB-412                                              Genetics                                          3(2-0-2)

Mendels law of heritability, mono, di hybrids ratios
Linkage: types, linkage maps, linklage groups, coefficient of coincidence, coefficient of interference
Crossing over, cytological basis of crossing over, factors effecting crossing over, estimation of
recombinatrion frequency from f2 data.
Practicals:
Simple and compound microscope, cell culture. Monohybrid and dihybrid ratios, test cross, back cross,
epistatic interactions, practice of mitotic and meiotic cell division, study of special chromosomes, probality,
chi square and crossing over, two point test, cross linkage analysis, three point test, cross linkage analysis


 MBMT-401                                       Microbiology -II                                        3(2-0-2)
Plant Microbe association symbiotic associative and asymbiotic nitrogen fixation, Azolla and Mycorrhiza
Biodegradation of agricultural chemicals: pesticide, herbicides and agricultural organic wastes
Carbon, nitrogen, phosphorous and sulphur cycles
Microbes in human welfare, biofertilizers, pesticide, waste treatment
Rumen microbiology and silage production
Practicals:
Microbial analysis of soil water and air
Microbial analysis of fresh foods, fermented foods and vegetables.

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 COMP- 302                                Information Technology                                      3(2-0-2)
Introduction to windows: Start menu and accessories, windows explorer, my computer. Introduction to
networking of computers.
Internet-internet, TCP/IP, IP address, Basic C and C++,Oracle.
Information networks: Internet, world wide web, web browsers, HTTP, HTML and URLS, EMB.



 ME-419                           Principles of Heat and Mass Transfer                                3(3-0-0)
Mass Transfer Operation: Classification of mass transfer operation, choice of operation method, methods of
conducting the mass transfer operation, unit systems
Diffusion in solids: Ficks law diffusion, types of solid diffusion
Equipment for gas liquid operations: L.gas dispersed –sparged vessel (bubble columns), mechanically
agitated vessels, mechanical agitation of single phase liquids, gas liquid contract, tray tower, liquid dispersed
–venturi scrubbers, wetted wall tower, spray tower and spray chamber, packed tower. Concurrent tower gas
and liquid, end effect and artificial and tray mixing, tray tower vs. packed towers
Humidification operations
Distillation: Vapors liquid equilibria, single stage operation (flash vaporization) differential or simple
distillation, continuous rectification (binary system) multistage tray towers, continuous contact equipment
(packed towers) multicomponent systems, low-pressure distillation.
 Drying: Drying operation, batch drying, mechanism of batch drying, continuous drying, leeching


 BIOT-402                                         Enzymology                                          3(2-0-2)
Introduction to enzymes: general properties and significance, classification, nomenclature, terms and
definitions in enzymology – enzyme activity, specific activity, turnover number, active side, isoenzyme,
marker enzyme etc factors effecting enzyme activity, ph, temperature, substrate concentration isolation,
purification and localization of enzymes
Enzyme kinetics – derivation of Michaelis – menten equation, mechanism of bisubstrate and multisubstrate
catalyzed reactions, regulation of enzyme activity, enzyme inhibition Mechanism of enzyme action and
concept of active site, lysozyme, chymotrypsin, dehydrogenaze, proteases, lipases, aldolases, mode of action
of multienzyme, extracellular enzymes zymogens, entremozymes, abzymes, ribozymes
Enzyme technology –commercial production of enzymes, techniques involved in enzyme assay,
immobilization of enzymes, examples of enzymes, engineering application of enzymes (therapeutic use,
analytical use, manipulative use etc) uses of enzymes in solutions, enzyme reactors, biphasic systems
Practical
  Determination of proteins
  Salivary amylase activity
  Effect of salt on salivary amylase
  Effect of ph on enzyme activity
  Acid/alkaline phosphatases
  Activity of extracellular enzymes such as protease, oxidase, urease, catalase and lipase




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 BCBT-440                        CHEMICAL THERMODYNAMICS                                           3(3-0-0)

INTRODUCTION TO THERMODYNAMIC TERMS (system, surrounding, boundries etc) open and closed
loop systems, isolated systems, thermodynamic variables, extensive and intensive properties –2l
Thermodynamic process – lost thermal, adiabatic process, isobaric, isoclaric, cyclic, cyclic and irreversible
process 2l
Laws of thermodynamics –thermal equilibrium, zero law, first law, second law of thermodynamics, energy
concept, law of conservation of energy, concept of enthalpy, entropy –3L.
Reactive systems, degree of reaction, reaction equilibrium, laws of mass action, gibbs function change heatof
reaction, fungacity + reactivity, construction, enthalpy of formation. Laws for reaction systems –3L
Air cycle, otto cycle, work done in cycles, thermal efficiency –3l
Refrigeration – introduction to principles of vapour compression cycle –2l



 CBAS-400                                       TRAINING-I                                       1(0-0-2)




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                                               SEMESTER-V


 ECE-430                     Electronic measurement & Instrumentation                                3(2-0-2)
Semiconductors: energy band theory of solids, concept of solids, concept of forbidden gaps, insulators and
semiconductors. Transport phenomenon in semiconductors, instridic, and extrinsic semiconductors
Junction diode p-n junction diode vi characteristics, diode application as a rectifier (half wave and full wave)
breakdown mechanism –se4len and Avalenche characteristics, zener diode and its applications LED and its
applications
Bijunction transistor, CE, CB Configuration, characteristics, curves, requirement of biasing, stability, small
signal equivalent ckt, h- parameter model, transistor as an amplifier, frequency response of amplifiers,
negative feed back, gain band width product, oscillators and condition for oscillation
Other semiconductor devices: introduction of JFET, MOSFET, and CMOS
Switching theory and logic gates number systems, Boolean algebra, logic gates, flip flop and ICS
A/D and D/A converters introduction, methods of conversion A/D, D/A
Quantities of measurements: introduction, performance characteristics, static characteristic, error in
measurement, types of error, and source of error, dynamic characteristic
 Electronic instruments: ammeter, voltmeter, ohm meter, VOM, Q meter
CRO block diagram and working
Transducers and measurements of electrical and non electric quanta ties: measurement of liquid level, flow
temperature, strain, pressure, force, torque, power, displacement, vibration, acceleration due to transducers.
Measurement of resistance, inductance, capacitance using DC (Wheatstone bridge) and AC (MAXWELL
Bridge, WEINBRIDGE) bridges.
List of practicals:
Study of diode characteristics
Study of half wave and full wave rectifier with and without capacitor filter and determine ripple factor


 BCBT-452                                     Basic Immunology                                       3(2-0-2)
Basic terminology: infection, pathogen, antigen, bacterial and viral antigens, haptens, adjuvents, immunogen,
interferons, antibody etc
Specific and non specific immune response, cellular base of immunity, cells of immune system, B& T cells,
macrophages, defence mechanism, phagocytosis and inflammation
Histocompatability complex (MHC-I, MHC-II) structure and function
Structure of T-cell receptors and the compliment system – mode of activation, its function, autoimmunity and
hypersensitivity
Principles of RIA, ELISA and immunoelectrophoresis, monoclonal antibodies used in immunodiagnostics and
immunotherapeutic.


 MBGE-441                                    Molecular Genetics                                      2(2-0-0)
Gene concept and genetic analysis: prokaryotic, viral and eukaryotic genome, concept of gene, genome
mapping, concept of central dogma
Transcription of RNA molecules, enzymatic synthesis of RNA, Gene splicing, mechanism of transcription
process
Translation: Introduction to protein synthesis, codon –anticodon interaction, genetic code and its
characteristics, post translational modification of proteins
Gene regulation: Gene regulation in prokaryotes (lac operon and trypton operon model) gene regulation in
eukaryotes




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 MBGE-451                                    Plant Biotechnology                                     3(2-0-2)
Plant genome: nuclear genome, chloroplast genome nuclear genome, chloroplast genome and mitochondrial
genome
Introduction to plant biotechnology: selected definitions in biotechnology, scope, area and development of
biotechnology in India. Methods of gene transfer in plants
Analysis and expression of cloned genes
Gene transfer system in plants: Agrobacterium tumefaciens, plant viral vectors, other methods of direct gene
transfer, problems
Plant interaction, application of vam for disease resistance, microbial application of seeds, pathogenic plant-
microbe interactions, phytoalexins, allelopathy.


 MBGE-452                              Environmental Biotechnology                                   2(2-0-0)
UNIT I: Introduction of Environmental Biotechnology and its importance and role in environment protection.
UNIT II: Ecosystem, Population and Succession and Biodiversity
UNIT III: Introduction of Bioremediadion, Bioleaching, Biodegradation, Blooming Phytoremediation,
Biostimulation, Bioaccumulation, Bioaugmentation, Biomagnification.
UNIT IV: biotechnological methods of pollution detection, biological indications ,bioassay and biosensors.
UNIT V: Sewage and Soil waste management.
UNIT VI: Global Environment issues –Ozone depletion, Green house effect ,Acid rain ,Sea level rise.
UNIT VII: Environmental Hazards, Human


 BCBT-452                                  Chemical Engineering                                      3( 3-0-0)
Mixing: Types of agitator, flow pattern,and power consumption
Steady state conduction: Fourier’s laws , concept of resistance to heat transfer, critical insulation thickness,
conduction with heat generation.
Convection:Film theory and concept of heat transfer coefficient. Heat transfer in laminar and turbulent flows.
Heat exchanger: Sizing of shell and tube heat exchanger. Heat transfer in agitated vessel.
Boiling and condensation: Heat transfer to boiling liquids and from condensing vapours.
Fundamentals of mass transfer: Molecular diffusion in fluids and solids, concept of mass transfer
coefficient.Equilibrium stage, multistage and continuous contractors with application to gas absorption ,
calculation of NTU , HTU and no. of stages.
Psychrometric chart and its application

 BI-490                                         Bioinformatics                                       3 (2-0-2)
Introduction to Bioinformatics. Various definitions of Bioinformatics.
Bioinformatics and pharmaceutical industry.
A chronological history of Bioinformatics.
Bioinformatics in industry. Internet and Bioinformatics.
Knowledge discovery and data mining in Bioinformatics.
Problem faced in Bioinformatics area.
Bioinformatics – employment opportunities.
The human genome project –influence area.
Introduction to Linux operating system –installation and getting familiar with its working environment.
Oracle for windows (SQL databases).
Structure and classification of databases.
Bioabacus categories: Bioinformatics recourse.
Getting familiar with other packages for Linux environment.
Information networks Internet, World Wide Web, web browsers, http, html and urls, emb net ncbi, web
addresses for some Bioinformatics organizations.
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 MBGE-454                    Molecular Techniques and Instrumentation                                3(2-0-2)
Molecular techniques- isolation and purification of DNA, RNA and plasmid DNA DNA finger printing,
polymerase chain reaction (PCR)
Chromatography: adsorption, partition, exclusion, ion exchange chromatography, affinity chromatography,
HPLC, FPLC, Column chromatography, chromatofocussing
Electrophoresis, principles and applications, gel electrophoresis (agarose and PAGE), PFGE (pulsed field gel
electrophoresis) centrifugal techniques: differential centrifugation, zonal and density gradient centrifugation,
ultra centrifugation
Radioisotopic and other techniques: detection and measurement of isotopes (GM and Scintillation counters),
autoradiography
Principles instrumentation and applications: colorimetry, spectrophotometry, and atomic absorption
photometry
Practical
Preparation of gels (agarose, PAGE) for electrophoresis
Separation of proteins and DNA by electrophoresis
Protein estimation by Lowry and biuret methods
Identification of amino acids by paper chromatography
Separation of amino acids by column chromatography

 LNG-304                Professional Communication and Technical Writing                             3(3-0-0)

* Language
Syntax
Kinds of incomplete Sentences
Auxiliaries and Infinitives (Tense)
Modifiers and Comparison
Connectives
Determiners
* Technical Writing
Importance
Process of Writing
Essentials (Technical and Grammatical)
Letters and other communication
Reports
Technical Articles
Proposal (Format and Presentation)
* Speech Skills
International Phonetic Alphabets 44.
Strong and Weak Forms.
Transcription of words and Sentences.




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                                                SEMESTER VI


 MBGE-501                 Bio-safety and Bioethics and IPR in Biotechnology                             3(3-0-0)
General Introduction, Patent claims, Ownership of Tangible and Intellectual property.
IPR for Biological material: International Conventions and International Cooperation, International
Organization, WTO, TRIPS.
Cortagena protocol on Biosafety, Biosafety management, Ethical implications of Biotechnological products
and techniques.
Social and Ethical Implications of Biological weapons.


 BAM-502                          Marketing of Biotechnology Products                                   3(3-0-0)
Concept of Marketing
Customer Satisfaction and Buyer Behavior.
Marketing Mix
Analyzing Consumer Markets of Biotechnological products.
Pricing-strategies and methods of pricing of Biotechnological products
Identifying Markets Segments and Selecting Target Markets for Biotechnological products.
Positioning the Market Offering Through the Product Life Cycle
Distribution Channel
Promotion of Biotechnological Products

 TC-502                                          Tissue Culture                                         3(2-0-2)
Tissue culture – a historical view, organization of tissue culture laboratory, aseptic techniques, media
formulation, clonal propagation vs tissue culture, totipotency, growth differentiation and morphogenesis in
tissue culture various types of aseptic cultures and culture techniques. Application of tissue culture
techniques: somoclonal and gametoclonal variation, protoplast fusion and somatic hybridization,
cybridization, haploid and monoploid production, secondary transformation, production of metabolites,
artificial seeds, preservation of genetic resources, cryopreservation, application of tissue culture for plant
improvements


 BCBT-501                                  Biochemical Engineering                                      3(2-0-2)
BIOCHEMICAL ENGINEERING – bioreactors definition, classification of chemical reactors, residence
time, bioreactors design and their functions, types of bioreactors. Application of bioreactors in biofertilizer
production, alcohol production, antibiotic production, plant cell culture, animal cell culture, waste
management
Control of bioprocess parameters in bioreactors:ph , temperature , pressure , oxygen supply , agitation ,
nutritional , on / off control, PID control, control of heating of bioreactors ,flow measurement controls ,
chemical and electrical methods of foam control, off line control parameters ( measurement of properties
analysis of cell population and composition) use of computer technology in bioprocess control systems
Engineering principles: transport phenomenon in bioprocess, mass transfer principles, mass transfer
coefficient in gases and liquids, heat transfer principles, coefficient of heat transfer, applied enzyme catalysis,
biomass production in cell cultures
Upstream and downstream processing: media formulation, sterilization of equipments, gas compressor types
and principles of compression, air filtration, solid and liquid handling. Industrially fermented broth (filtration
and ultra filtration) centrifugation, solvent extraction, chromatographic separation, liquid extraction of
biopolymers and antibiotics ion exchange recovery of antibiotics and proteins




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 MBGE-502                            Recombinant DNA Technology                                     3(2-0-2)
Biology of cloning vectors: plasmids, cosmids, lambda bacteriophage, phagemid, Agrobacterium tumefaciens
Enzymes used in genetic engineering, restriction endonucleases, reverse transcriptase , terminal transferases ,
ligases , exonucleases , s1 nuclease kinases , polymerases , alkaline phosphatase
Principles of recombinant DNA technology: gene splicing, construction of R- DNA
R-DNA expression: genomic and complimentary DNA (c-DNA) libraries, detecting expression of foreign
genes, application of R- DNA technology
Gene cloning in mammalian cells: factors effecting cloning in mammalian cells, reporter gene assays –
luciferase, galactosidase . transfection methods – protoplast fusion , electroporation , microinjection


 BCBT-502                                Industrial Biotechnology                                   3(2-0-2)
Application of biotechnology in pharmcecutical, food, beverage, leather, paper, textile, detergent and
chemical industries
Biotechnology industry and medicine: Antibiotics, therapeutic applications, Developing recombinant proteins
for medical and therapeutic use, drug designing, new drug targets and vaccine development- Insulin, anti-
haemophilic factor, human serum albumin, engineered enzymes
Food and beverage industry: Xylanases, emulsifiers, high intensity sweetener, foods from microbes, edible
mushrooms, role of biotechnology to improve food quality and food production
Fermentation, cultures of microorganisms, solid or semisolid culture, batch culture, continuous culture,
fedback culture, metabolic pathways in microorganisms, glycolysis or EMP pathways, entner-doudoroff
pathways, the pentose phosphate pathways


 MBMT-501                              Microbial Biotechnology                                      3(3-0-2)
Introduction of recombinant vector into the bacterial cell (expression of cloned genes in E. coli, selection of
clones: colony hybridization techniques, plaque hybridization technique southern blotting technique
Transformation of filamentous fungi, gene transfer through Ti plasmid, gene transfer through gene gun
Application of gene technology – production of pharmaceuticals (insulin, recombinant vaccines, human
growth hormones, diagnosis of disease, DNA probe in diagnosis, uses of genetically engineered
microorganisms (GEMS) in control of pollution.


 MBGE-453                                  Animal Biotechnology                                     3(3-0-2)
BASIC ANIMAL GENETICS: chromosomes as vehicles of heredity, various types of chromosomes in
animals an d livestock, structure of chromosomes, chromosome banding, gene function and controlling
eukaryotes
Human chromosomal abnormalities: kleinfelter syndrome, autosomal abnormalities, trisomy and use of
nucleic acid probes and antibodies in clinical diagnosis, tissue typing single cell anemia
Gene mapping and gene cloning: various methods of gene mapping, human genome project, RFLP and
applications, gene map for man, mouse and other animals
Basic strategies and methods in gene cloning
 Genetic engineering of animal cells: gene isolation from animals, cloning and expression vectors, animal
viral vectors and yeast vectors, production of transgenic animal and chimeras, gene transfer technology in
animals. Ethical issues in animal biotechnology


 BAS-488                                          Seminar- 1                                        1(0-0-2)




 BAS-500                                          Training II                                       1(0-0-2)


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                                             SEMESTER VII

                                GENETIC ENGINEERING(GE)

 MBGE-601                                   Molecular Breeding                                     3(2-0-2)
Different kinds of markers: morphological, biochemical and molecular markers, PCR and its approaches
Molecular marker system; non-PCR based approaches (RFLP), PCR BASED techniques {(RAPD, SSR,
AFLP, APPCR) TARGETED PCR (STS, SCAR etc)
Marker assisted selection (MAS). Development of markers, generation of marking population. Bulk
segregates analysis, use of NILS for tagging genes. Selection of QTLS for MAS
Uses of molecular markers in plant breeding: resistance breeding, gene interogression, gene pyramiding,
abiotic resistance, and improvement of quaklitatative characters, fertility restorer genes and hybrid vigor.


 MBGE-602                                         GEnomics                                         3(3-0-0)
Introduction and basic concepts of genomics, proteomics, transcriptomics and metabolomics
Genomics: genome, genome projects, genetical and physical maps, automated DNA sequencing, DNA micro
array, gene finding, genome databases and data mining structural, functional and comparative genomics.


 BI 602                                  Biomolecular Modeling                                     2(1-0-1)
Introduction to molecular modeling and protein folding problem.
Protein architecture and different level of structural complexity.
Empirical methods for structure determination-X-ray crystallography , NMR(Nuclear Magnetic Resonance)
Computational method for structure determination-ab initio methods and knowledge based method.
Protein Structure Prediction with Artificial Neural networks.
RNA Structure Prediction.
Computer Simulation.
Application of Modeling-Protein Ligand Docking/Protein Protein Docking QSAR( Quantitative string
activity relationship), Drug Designing


 MBGE-603                              Genetically Modified Foods                                 3( 3-0-0)
Content: What are genetically modified foods? What are some of the advantages of GM foods? How
prevelant are GM crops? What are some of the criticisms against GM foods? How are GM food regulated and
what is the government role in this process? How are GM food labeled?


 MBGE-604                          Gene Cloning And Gene Therapy                                   3(3-0-0)
Introduction to gene cloning: basic events of gene cloning, cloning vehicles and vectors – plasmids, cosmids,
and phage vectors, transposons, YAC, BAC
Enzymes for cutting and joining DNAmolecules. Enzymes for cutting restriction endonucleases. Enzymes for
joining DNA molecules, linkers and adapters
Gene libraries: cDNA library and cloning. Genomic cloning, identification and analysis of cloned genes
DNA sequencing; sequencing methods – Maxam and Gilbert method, Sanger dideoxymethod and its
modifications novel sequencing methods – scanning tunneling microscopy sequencing strategy in genome
projects.
Gene Therapy:General introduction to Gene therapy, Types and applications.



 BAS-589                                         Seminar-II                                        1(0-0-2)

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                                MICROBIAL TECHNOLOGY

 MBMT-601                                Fermentation Technology                                     3(2-0-2)
Metabolic Engineering: Basic concept of metabolic engineering, different model for cellular reaction,
metabolic regulation network at enzyme level and whole cell level, metabolic network, thermodynamics of
cellular process, new concept for quantitative bioprocess research and development.
Fermentation Technology: Introduction to fermentation process, basic definition of fermentation, historical
landmarks, various classes of fermentation difference between anaerobic respiration and fermentation.
Production techniques of commercial molecular through fermentation technology, organic acid and amino
acids.
Food Fermentation- Lactic acid fermentation of cabbage, fermented sausage and products.
Dairy Fermentation- Fermented milks and beverage.
Practicals:
Yeast fermentation to produce ethanol.
Isolation of milk protein.
Enzymatic test to determine the quality of milk.
Carbohydrate metabolism.
Carbohydrate fermentation.
Introduction of lypolytic microorganism from butter.
Amylase production Test.



 MBMT-602                             Microbial Process Engineering                                  3(2-0-2)
Bioprocess Development: An interdisciplinary challenge, major classes of products.
Introduction to Engineering Calculation, Presentation and analysis of data: Physical Variables, dimensions,
units, errors in data and calculations, testing mathematical models, process flow diagram.
Material Balances:Thermodynamic law of law of conservation of mass, types of material balance products,
electron balance, biomass yield, theoretical oxygen demand, problems.
Energy Balance: Basic concepts, general enrgy balance equations, enthalpy calculations, enthalpy in non
reactive process, types of energy balance calculations, types of heat reactions, problems.
Unsteady state material and energy balances: Material balance equation for CSTR, Energy balance equation,
solving differential equations, solving mass balance, solving energy balance, problems.
Fluid Flow and mixing : Classification of fluids, Reynolds number, Momentum Transfer, Non- Newtonian
fluids, Two parameter models, rheological properties of fermentation broths, mixing, power requirement for
mixing, scale up of mixing systems, role of shear in stirred fermentors, problems.
Heat Transfer: Equipments, mechanism of heat transfer, conduction, haet transfer between fluids, design
equation for heat transfer systems, application of design equations, problems.
Mass Transfer: Molecular diffusion, role of diffusion in bioprocessing, film theory, convective mass transfer,
oxygen uptake and transfer in cell cultures, kLa determination, problems.
Homogenous reactions: Basic reaction theory, calculation of reaction rates, general reaction kinetics for
biological systems, yield in lab cultures.
Heterogenous reactions: Concentration gradients and reaction rates in solid catalysts, internal mass transfer
and reaction, the thiele modulus and effectiveness factor, external mass transfer, problems.
Reaction Engineering : Bioreactor configurations, practical considerations for bioreactor construction,
monitoring and control of bioreactors, ideal reactor operations, batch operation of a mixed reactors, case study
of penicillin production.




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 MBMT-603                                Clinical Biotechnology                                  2(2-0-0)
History and evolution of Medical Biotechnology
Infectious diseases: Viral, bacterial, parasitic, Mycobacterial.
Host-parasitic relationship
Methods of culturing and assaying: bacterial, viral and parasitic
Bacterial and viral vectors
Biological warefare agents
Replication of DNA, RNA+ve, RNA-ve Viruses, retroviruses
Hospital acquired infection (nosocomial)
Mode of action of antibiotics, antivirals: Molecular mechanism of drug resistance (MDR)
Clinical applications of DNA based diagnostics.



 MBMT-604                           Microbial Enzyme Technology                                  2(2-0-0)
Enzymes and its classification
Enzymes produced by bacterial and fungi
Cell / enzyme Immobilization
Production of microbial enzymes:
                 a. Recent developments and present position
                 b. Strain selection and development
                 c. Fermentation Processes
                 d. Recovery and Finishing
Application of Microbial Enzymes
Commercial production of microbial enzymes
                 e. Detergent enzymes
                 f. Starch processing enzymes and related carbohydrates
                 g. Enzymes in cheese production
                 h. Enzymes in plant juice production
                 i. Enzymes in textile manufacture
                 j. Enzymes in leather manufacture
                 k. Enzymes in treatment of wood pulp
Regulations and specifications for microbial enzymes



 MBMT-605                      Microbial Technology in Food Industry                             2(2-0-0)
Food as a substrate for microorganisms
General principles underlying spoilage of foods and different methods of preservation of foods.
Fermented foods : types, production and defects.
Starter Cultures.
Brewing, malting, mashing, hops & primary and secondary fermentation.
Microbial role in food processes, operation and production; new protein foods – SCP, mushroom, food yeasts,
algal proteins.
Quality and safety assurance in food and dairy industry.



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                             BIOCHEMICAL TECHNOLOGY (BT)

 BCBT-601               Bioprocess Engineering & Down Stream Processing                            3(2-0-2)
Microbial biomass, single cell proteins and its nutritional values, bakers yeast brewers yeast food and fodder
yeast, carbohydrates –whey molasses starch cellulose / wood waste, sulfite liquor, SCP production technology
bacterial protein (ICI) process yeast protein actionomyceatous protein, mycoproteins , algal proteins
(Spirulina cultivations )
Production of antibiotics vitamins definition, classification of antibiotics and biochemistry, penicillin,
streptomycin, tetracycline’s, geriosofulvin, cephalosporin, ampicillin, piocyanase, vitamins –A,
Riboflavin,cephalosporin,valinomycin ,carotenoides
Solvents, biopolymers and microbial insecticides solvents, ethylalcohol, glycerol,acetone , butanol , 2,3
butandiol . Biopolymers – expolysacharides , alaganides xanthan , dextran ,curdlan polyhydroxybutrate
Computer control of fermentation process of computer, hardware and software application in fermentation
technology, fermentation economics, fermentation in biofertilizer production, fuel alcohol production, biogas
production technology,silage production ,aspartame



 BCBT-602             Immmunology Vaccine & Transplantation Technology                             3(2-0-2)
Overview: Concept of self and non-self, antigen, antibodies, immune response, evolution of immune
response, immunological tolerance, hypersensitivity, humoral and cell mediated immunity, active and passive
immunization.
Antibody immunoglobin: Classes and subclasses, Structure-function relationship, isotypes , idiotypes and
allotypes.
Immunobiology: Development of immune system, development of lymphoid tissue , cellular basis of
immunity , T-cell receptor.
Antigen-antibody reaction, Complement fixator, agglutination, immunoelectrophoresis immunoflourescence,
ELISA, RIA.
Immunogenetics: Blood groups, transplantation antigen , HLA and disease association , immunodeficiency
disorder , immunological diseases (AIDS) , antigen processing and MHC.
Allergy, immunoprophylaxis, vaccines and MABs



 BCBT-603                          Medical and Molecular Diagnosis                                 2(1-0-2)
General Clinical laboratory techniques an procedure: Chemical and related substrates, volumetric analysis,
balancing an weighing, concept of solute and solvent, units of measurement.
Specimen collection and processing: Specimen collection( Blood, urine, spinal fluid, saliva synovial fluid,
amniotic fluid), preservation, transportation.
Selection and interpretation of lab procedure: Classification of BIAS, sensitivity and specificity, Receiver
Operator characteristics.
Quality Management: Fundamentals of total quality management, Element of QAP, external quality
assessment and proficiency testing programme.
Clinical Enzymology: Principle of diagnostic enzymology, Liver, cardiac and skeletal enzyme, Digestive
enzyme, miscellaneous enzymes.
General function Tests: Liver function test, Cardiac function test, renal function test, Thyroid function test
Immunodiagnostics: Introduction, Ag-Ab reactions, Conjugation techniques, Ab- production, enzymes and
signal amplification systems.
DNA based Diagnostics: PCR, RFLP, SSCP, micro arrays, FISH, in-situ hybridizations, Case studies related
to bacteria, viral and parasitic infections.
Cell based diagnosis: Ab marker, CD markers, FACS, HLA typing, Bioassays.
Biosensors.


                                                        24                       Mandatory Disclosure Report 2009-10
    Allahabad Agricultural Institute-Deemed University

 BCBT-604                           Pharmaceutical Biotechnology                                2 (2-0-0)

Genetic recombination: Transformation, Conjugation, transduction, protoplastn fusion and gene cloning and
their application. Development of Hybridoma for monoclonal antibodies. Study of drugs produced by
biotechnology such as Activse, HumstropeIntorn A. Monoclate, ORTAHOCLONE OKT3, referon-A,
Recomboivax HB ETC.
Microbial transformation: Introduction, types of reaction mediated by micro-organisms, design of
Biotransformation processes and its improvements.
Enzyme transformation: Introduction, methods and immobilized enzymes, Techniques of immobilizationof
enzymes, Immobilization of bacteria and plants cells.

 BCBT-605                                  Biopolymer Technology                                  2(2-0-0)

General introduction – Introduction , classification of polymer , nomenclature and isomerism of polymer.
Synthesis of polymers , chain growth polymerization( addition polymerization ) – Introduction , mechanism
of polymerization(free radical, ,cationic , anionic), coordination Polymerization , Ring Opening
polymerization , Phase systems in polymerization(techniques of polymerization) , industrial polymerization.
Synthesis and application of some common industrial polymers.
Behavior of polymers: Crystalline, Thermal, Dilute solution, Rheological, Chemical.
Polymer Technology:Physical properties versus application, plastics, fibers, elastomers, adhesive, polymer
additives.
Polymer Processing: Casting, thermoforming, Foaming, Lamination, reinforcing, processing of fibers,
moulding processes.
Natural Polymers: Polysaccharides, Proteins, nucleic aciod, natural rubber.
Inoraganic Polymers: Silicones, Polyphosphazenes, orgametallic polymers, coordination polymers.



 BAS-589                                        Seminar II                                       1(0-0-2)




                                           SEMESTER VIII


 BAS-699                                  Project (GE /IM/BT)                                  9(0-0-18)




                                                      25                       Mandatory Disclosure Report 2009-10

								
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