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					                                                                    info@biomol.de                0800-2466651 www.biomol.de


                          Adducin Alpha Phospho (pS481)                                          Cat #: 1762-1      Size: 100ul         min. 50 slides
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                             Type:        Rabbit Monoclonal IgG              Clone:         EP733Y            Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                     Localization:     Cell Membrane,          Control:         Muscles
Primary Antibodies




                                                                                            Cytoplasmic


                                                                                                    Description: Adducin is a membrane skeletal protein that
                                                                                                    binds to actin filaments (F-actin) and promotes the as-
                                                                                                    sociation of spectrin with F-actin to form a spectrin-actin
                                                                                                    meshwork beneath plasma membranes. Adducin binds
                                                                                                    with high affinity to Ca 2+/calmodulin and is a substrate for
                                                                                                    protein kinase C PKC), and protein kinase A PKA). PKA
                                                                                                    specifically phosphorylates Ser408, Ser436, and Ser481
                                                                                                    located in the neck domain of alpha Adducin. Phosphoryla-
                                                                                                    tion of Adducin alpha by Rho-kinase at Thr445 and Thr480
                                                                                                    enhances the F-actin-binding activity of Adducin alpha.




                                                                                                    Immunogen: A synthetic phospho-peptide corresponding
                                                                                                    to residues surrounding Ser481 of human alpha Adducin
                          Paraffin-embedded human uterine myometrium tissue stained with            was used as an immunogen. The antibody only detects
                          anti-Phospho-Adducin-alpha RabMAb. Note positive staining of smooth       alpha Adducin phosphorylated on Serine 481.
                          muscles




                          AGR2                                                                   Cat #: 2533-1      Size: 100ul         min. 50 slides
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                             Type:        Rabbit Monoclonal IgG              Clone:         EPR3279           Species Reactivity: Human, Rat

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic             Control:         Colon,
                                                                                                                                     Breast carcinoma


                                                                                                    Description: The anterior gradient protein-2 (AGR2) is a
                                                                                                    cancer cell marker specifically up-regulated in response to
                                                                                                    depletion of serum and oxygen. It has been identified as a
                                                                                                    tumor marker in primary and secondary cancer lesions, and
                                                                                                    as a marker for detection of circulating tumor cells. El-
                                                                                                    evated levels of AGR2 are known to increase the metastatic
                                                                                                    potential of cancer cells. Specifically, AGR2 may serve as a
                                                                                                    useful molecular marker and/or potential therapeutic target
                                                                                                    for hormone-responsive breast tumours. AGR2 expression
                                                                                                    promotes tumor growth in esophageal adenocarcinoma
                                                                                                    cells as well. Expression was detected in proliferating and
                                                                                                    differentiated intestinal cells of secretory lineage, suggest-
                                                                                                    ing that AGR2 may be important for the growth and devel-
                                                                                                    opment of the intestine as well as esophageal adenocarci-
                                                                                                    nomas.

                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues in human AGR2 was used as an immunogen.
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue
                          stained with anti-AGR2 RabMAb. Note positive staining of tumor cells




                     10    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                        info@biomol.de                0800-2466651 www.biomol.de


AGR2                                                              Cat #: 2574-1        Size: 100ul         min. 50 slides
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   Type:        Rabbit Monoclonal IgG            Clone:        EPR3278           Species Reactivity: Human




                                                                                                                                             Primary Antibodies
IHC Dilution:   1:250 - 500                   Localization:    Cytoplasmic             Control:         Colon,
                                                                                                        Breast carcinoma


                                                                       Description: The anterior gradient protein-2 (AGR2) is a
                                                                       cancer cell marker specifically up-regulated in response to
                                                                       depletion of serum and oxygen. It has been identified as a
                                                                       tumor marker in primary and secondary cancer lesions, and
                                                                       as a marker for detection of circulating tumor cells. El-
                                                                       evated levels of AGR2 are known to increase the metastatic
                                                                       potential of cancer cells. Specifically, AGR2 may serve as a
                                                                       useful molecular marker and/or potential therapeutic target
                                                                       for hormone-responsive breast tumours. AGR2 expression
                                                                       promotes tumor growth in esophageal adenocarcinoma
                                                                       cells as well. Expression was detected in proliferating and
                                                                       differentiated intestinal cells of secretory lineage, suggest-
                                                                       ing that AGR2 may be important for the growth and devel-
                                                                       opment of the intestine as well as esophageal adenocarci-
                                                                       nomas.

                                                                       Immunogen: A synthetic peptide corresponding to resi-
                                                                       dues in human AGR2 was used as an immunogen.
Paraffin-embedded human endometrium adenocarcinoma tissue stained
with anti-AGR2 RabMAb. Note positive staining of tumor cells




AIF                                                               Cat #: 1020-1        Size: 100ul         min. 50 slides
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   Type:        Rabbit Monoclonal IgG            Clone:        E20               Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 250                   Localization:    Cytoplasmic             Control:         Cervical carcinoma,
                                                                                                        Breast carcinoma


                                                                       Description: AIF, or apoptosis-inducing factor, is a flavopro-
                                                                       tein localized to the mitochondrial membrane that plays a
                                                                       primary role in caspase-independent cell death, or apopto-
                                                                       sis. AIF translocates to the nucleus when apoptosis is in-
                                                                       duced and causes mitochondria to release the apoptogenic
                                                                       proteins, cytochrome c and caspase-9. Apoptotic activity of
                                                                       AIF leads to chromatin condensation and DNA fragmenta-
                                                                       tion . Similar to cytochrome c, AIF is a bifunctional protein
                                                                       with an oxidoreductase function and a second apoptogenic
                                                                       function. In contrast to cytochrome c, however, AIF acts in a
                                                                       caspase-independent fashion.




                                                                       Immunogen: A synthetic peptide correspndonding to
                                                                       residues near the C-terminus of Human AIF was used as
Paraffin-embedded human cervical adenocarcinoma tissue stained with    immunogen.
anti-AIF RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                     www.epitomics.com                   info@epitomics.com                        11
                                                                  info@biomol.de                 0800-2466651 www.biomol.de


                          Akt1 Phospho (pS473)                                              Cat #: 2118-1         Size: 100ul          min. 50 slides
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                             Type:        Rabbit Monoclonal IgG            Clone:        EP2109Y            Species Reactivity: Human, Mouse

                          IHC Dilution:   1:100 - 250                   Localization:    Cytoplasm,               Control:          Breast carcinoma,
Primary Antibodies




                                                                                         Nuclear                                    Ovarian carcinoma


                                                                                                  Description: Akt1/PKBa is a Ser/Thr protein kinase whose
                                                                                                  kinase activity plays a key role in various cellular functions,
                                                                                                  including apoptosis, glycogen synthesis, and cell growth.
                                                                                                  Akt1/PKBa is activated by various growth and survival
                                                                                                  factors . Phosphorylation on Thr308, Ser473 and Tyr474
                                                                                                  is required for full activity. Akt1/PKBa inhibits apoptosis by
                                                                                                  phosphorylating and inactivating several targets, including
                                                                                                  caspase-9. Akt1/PKBa regulates glycogen synthesis by
                                                                                                  phosphorylating and inactivating glycogen synthase kinase-
                                                                                                  3a and b. Three mammalian Akt1/PKBa isoforms have been
                                                                                                  identified. Over expression and abnormal expression of
                                                                                                  AKT is commonly seen in many types of cancer.




                                                                                                  Immunogen: A phospho specific peptide corresponding to
                                                                                                  residues surrounding Ser473 of human Akt1 was used as
                          Paraffin-embedded human ovarian adenocarcinoma tissue stained with      immunogen. The antibody detects Akt1 phosphorylated at
                          anti-Phospho-AKT RabMAb. Note positive staining of tumor cells          Serine 473.




                          Akt1                                                              Cat #: 1081-1         Size: 100ul          min. 50 slides
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                             Type:        Rabbit Monoclonal IgG            Clone:        Y89                Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100                         Localization:    Cytoplasmic,             Control:          Prostatic carcinoma,
                                                                                         Nuclear                                    Breast carcinoma


                                                                                                  Description: Akt1/PKBa is a Ser/Thr protein kinase whose
                                                                                                  kinase activity plays a key role in various cellular functions,
                                                                                                  including apoptosis, glycogen synthesis, and cell growth.
                                                                                                  Akt1/PKBa is activated by various growth and survival
                                                                                                  factors . Phosphorylation on Thr308, Ser473 and Tyr474
                                                                                                  is required for full activity. Akt1/PKBa inhibits apoptosis by
                                                                                                  phosphorylating and inactivating several targets, including
                                                                                                  caspase-9. Akt1/PKBa regulates glycogen synthesis by
                                                                                                  phosphorylating and inactivating glycogen synthase kinase-
                                                                                                  3a and b. Three mammalian Akt1/PKBa isoforms have been
                                                                                                  identified. Over expression and abnormal expression of
                                                                                                  AKT is commonly seen in many types of cancer.




                                                                                                  Immunogen: A synthetic peptide corresponding to resi-
                                                                                                  dues in C-terminus of human Akt1 was used as immuno-
                          Paraffin-embedded human prostatic adenocarcinoma tissue stained with    gen. Predicted to cross-react with mouse, rat and bovine,
                          anti-AKT RabMAb. Note positive staining of tumor cells                  based on sequence homology.




                     12    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                         info@biomol.de                 0800-2466651 www.biomol.de


Aldh1A1                                                            Cat #: 2052-1        Size: 100ul          min. 50 slides
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   Type:        Rabbit Monoclonal IgG             Clone:         EP1933Y           Species Reactivity: Human, Mouse, Rat




                                                                                                                                              Primary Antibodies
IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic             Control:         Liver



                                                                         Description: Usual human livers contain two major ALDH
                                                                         isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2.
                                                                         ALDH1A1 is an aldehyde dehydrogenase which oxidizes
                                                                         variety of aliphatic aldehydes to the corresponding carboxyl-
                                                                         ic acids. ALDH1A1 is highly expressed in embryonal tissue
                                                                         and hematopoietic stem cells (e.g. caner stem cells).




                                                                         Immunogen: A synthetic peptide corresponding to resi-
                                                                         dues near the C-terminus of human Aldh1a1 was used as
Paraffin-embedded human liver tissue stained with anti-AldH1A1           an immunogen.
RabMAb. Note positive staining of hepatocytes




Alk Phospho (pY1604)                                               Cat #: 1891-1        Size: 100ul          min. 50 slides
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   Type:        Rabbit Monoclonal IgG             Clone:         EP661Y            Species Reactivity: Human

IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic             Control:         Anaplastic lymphoma
                                                                 and/or Nuclear


                                                                         Description: ALK is a novel receptor protein-tyrosine kinase
                                                                         having a putative transmembrane domain and an extracel-
                                                                         lular domain. The 2;5 chromosomal translocation occurs
                                                                         in most anaplastic large-cell non-Hodgkin’s lymphomas
                                                                         arising from activated T lymphocytes. This rearrangement
                                                                         was shown to fuse the NPM nucleolar phosphoprotein gene
                                                                         on chromosome 5q35 to a previously unidentified protein
                                                                         tyrosine kinase gene, ALK, on chromosome 2p23. In the
                                                                         predicted hybrid protein, the amino terminus of NPM is
                                                                         linked to the catalytic domain of ALK. Also expressed in the
                                                                         small intestine, testis, and brain but not in normal lymphoid
                                                                         cells, ALK shows greatest sequence similarity to the insulin
                                                                         receptor subfamily of kinases. Unscheduled expression of
                                                                         the truncated ALK may contribute to malignant transforma-
                                                                         tion in these lymphomas.

                                                                         Immunogen: A synthetic phospho-peptide correspond-
                                                                         ing to residues surrounding Tyr1604 of human ALK was
Paraffin-embedded human anaplastic large cell lymphoma tissue stained    used as an immunogen. The antibody only detects ALK
with anti-Phospho-ALK RabMAb. Note positive staining of tumor cells      phosphorylated on Tyrosine 1604.




 Toll Free : 1-877-7RABMAB (772-2622)                       www.epitomics.com                   info@epitomics.com                       13
                                                                  info@biomol.de                  0800-2466651 www.biomol.de


                          Alk/p80                                                            Cat #: 4204-1         Size: 100ul         min. 50 slides
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                             Type:        Rabbit Monoclonal IgG             Clone:        SP8                Species Reactivity: Human

                          IHC Dilution:   1:100                          Localization:    Cytoplasmic              Control:          Anaplastic lymphoma
Primary Antibodies




                                                                                          and/or Nuclear


                                                                                                   Description: ALK is a novel receptor protein-tyrosine kinase
                                                                                                   having a putative transmembrane domain and an extracel-
                                                                                                   lular domain. The 2;5 chromosomal translocation occurs
                                                                                                   in most anaplastic large-cell non-Hodgkin’s lymphomas
                                                                                                   arising from activated T lymphocytes. This rearrangement
                                                                                                   was shown to fuse the NPM nucleolar phosphoprotein gene
                                                                                                   on chromosome 5q35 to a previously unidentified protein
                                                                                                   tyrosine kinase gene, ALK, on chromosome 2p23. In the
                                                                                                   predicted hybrid protein, the amino terminus of NPM is
                                                                                                   linked to the catalytic domain of ALK. Also expressed in the
                                                                                                   small intestine, testis, and brain but not in normal lymphoid
                                                                                                   cells, ALK shows greatest sequence similarity to the insulin
                                                                                                   receptor subfamily of kinases. Unscheduled expression of
                                                                                                   the truncated ALK may contribute to malignant transforma-
                                                                                                   tion in these lymphomas.

                                                                                                   Immunogen: Recombinant protein corresponding to a
                                                                                                   region, which spans the tyrosine kinase catalytic domain
                          Paraffin-embedded human anaplastic large cell lymphoma tissue stained    and part of the C-terminus of the NPM-ALK transcript.
                          with anti-ALKp80 RabMAb. Note positive staining of tumor cells




                          Alpha Actin (Smooth Muscle)                                        Cat #: 1184-1         Size: 100ul         min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:        E184               Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:250                          Localization:    Cytoplasmic              Control:          Leiomyosarcoma,
                                                                                                                                     Smooth muscles


                                                                                                   Description: Actins are highly conserved proteins ubiq-
                                                                                                   uitously expressed in all eukaryotic cells. They comprise,
                                                                                                   along with microtubules, a major component of the cyto-
                                                                                                   skeleton. Actin has been found to be expressed in at least
                                                                                                   six isomeric forms. It is expressed in heart and skeletal
                                                                                                   striated muscle tissue, and in certain smooth muscle tis-
                                                                                                   sues, regulating contractile potentials for these cells. It has
                                                                                                   been reported that actin is hyperphosphorylated in primary
                                                                                                   breast tumors. It has also been shown that actin dysfunction
                                                                                                   leads to certain types of heart failure. Smooth muscle alpha
                                                                                                   actin is of further interest because it is one of a few genes
                                                                                                   whose expression is relatively restricted to vascular smooth
                                                                                                   muscle cells. Actin is specific marker for tumors arisisng
                                                                                                   from smooth muscles and myoeplithelia cells.


                                                                                                   Immunogen: A synthetic peptide corresponding to N-
                                                                                                   terminus of human Alpha-actin 3 was used as immuno-
                          Paraffin-embedded human uterine myometrium tissue stained with anti-     gen. This antibody only detects actin in smooth muscle in
                          alpha-Actin (Smooth Muscle) RabMAb. Note positive staining of smooth     immunohistochemistry.
                          muscles



                     14    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                            info@biomol.de                  0800-2466651 www.biomol.de


AP2 gamma                                                                 Cat #: 2390-1           Size: 100ul         min. 50 slides
                                                                                                Inquire for Bulk

   Type:        Rabbit Monoclonal IgG                 Clone:            EP2692Y             Species Reactivity: Human




                                                                                                                                                        Primary Antibodies
IHC Dilution:   1:250 - 500                        Localization:        Nuclear                   Control:         Breast carcinoma



                                                                                  Description: Transcription factor gene AP2-C (AP-2
                                                                                  gamma) belongs to a family of four closely related genes.
                                                                                  The AP-2 transcription factor has been shown to play an
                                                                                  important role in the development of tissues of ectodermal
                                                                                  origin and has also been implicated in mammary oncogen-
                                                                                  esis. AP-2 gamma had been implicated in multiple functions
                                                                                  during proliferation and differentiation based on its expres-
                                                                                  sion pattern in trophoblast, neural crest, and ectoderm cells
                                                                                  in murine embryos. AP-2 gamma seems to be required
                                                                                  in early embryonic development because it regulates the
                                                                                  genetic programs controlling proliferation and differentiation
                                                                                  of extraembryonic trophectodermal cells.




                                                                              Immunogen: A synthetic peptide corresponding to resi-
                                                                              dues in human AP2 gamma was used as an immunogen.
Paraffin-embedded human breast ductal infiltrating carcinoma tissue
stainedd with anti-AP2C RabMAb. Note positive staining of tumor cells




Aurora B                                                                  Cat #: 1788-1           Size: 100ul         min. 50 slides
                                                                                                Inquire for Bulk

   Type:        Rabbit Monoclonal IgG                 Clone:            EP1009Y             Species Reactivity: Human

IHC Dilution:   1:250 - 500                        Localization:        Nuclear                   Control:         Tonsil



                                                                                  Description: Aurora B is a member of a novel family of
                                                                                  serine/threonine kinases that have been identified as key
                                                                                  regulators of the mitotic cell division process. Aurora-B is
                                                                                  known to be involved in the regulation of centrosome func-
                                                                                  tion, bipolar spindle assembly and chromosome segrega-
                                                                                  tion. Overexpression of Aurora B produced multinuclear-
                                                                                  ity and induced aggressive metastasis, suggesting that
                                                                                  overexpressed Aurora B has multiple functions in cancer
                                                                                  development.




                                                                              Immunogen: A synthetic peptide corresponding to resi-
                                                                              dues near the N-terminus of human AuroraB was used as
Paraffin-embedded human tonsil tissue stained with anti-Aurora B              an immunogen.
RabMAb. Note positive staining of proliferating cells in the germinal
center



 Toll Free : 1-877-7RABMAB (772-2622)                           www.epitomics.com                        info@epitomics.com                        15
                                                                      info@biomol.de                     0800-2466651 www.biomol.de


                          B7-H4 (VTCN1)                                                            Cat #: 2516-1          Size: 100ul         min. 50 slides
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                             Type:         Rabbit Monoclonal IgG                 Clone:         EPR1165             Species Reactivity: Human

                          IHC Dilution:    1:250 - 500                       Localization:      Cell Membrane             Control:          Tonsil,
Primary Antibodies




                                                                                                                                            Breast carcinoma


                                                                                                          Description: B7 family members and their receptors play
                                                                                                          critical roles in the regulation of antigen-specific immune
                                                                                                          responses. B7-H4 ligation to its receptor BTLA on T lym-
                                                                                                          phocytes results in inhibition of T-cell activation, cytokine
                                                                                                          secretion, and the development of cytotoxicity. Cell surface
                                                                                                          expression of B7-H4 protein was induced upon stimulation
                                                                                                          of T cells, B cells, monocytes, and dendritic cells in addition
                                                                                                          to a constitutive B7-H4 protein expression in lung and ovar-
                                                                                                          ian cancer. It has been hypothesized that B7-H4 protein is
                                                                                                          consistently overexpressed in primary and metastatic breast
                                                                                                          cancer.




                                                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                                                          dues in human B7-H4 was used as an immunogen.
                          Paraffin-embedded human tonsil tissue stained with anti-B7-H4 RabMAb.
                          Note positive staining of lymphocytes




                          Bcl-2                                                                    Cat #: 1017-1          Size: 100ul         min. 50 slides
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                             Type:         Rabbit Monoclonal IgG                 Clone:         E17                 Species Reactivity: Human, Mouse

                          IHC Dilution:    1:100 - 200                       Localization:      Cytoplasmic               Control:          Tonsils,
                                                                                                                                            Follicular lymphomas


                                                                                                          Description: The Bcl-2 family of proteins regulate apopto-
                                                                                                          sis by controlling mitochondrial permeability and release
                                                                                                          of cytochrome c. Bcl-2 is an anti-apoptotic protein that
                                                                                                          resides in the outer mitochondrial wall and inhibits release
                                                                                                          of cytochrome c. Over-expression of Bcl-2 has been shown
                                                                                                          to promote cell survival by suppressing apoptosis. It has
                                                                                                          been documented that Bcl-2 becomes deregulated in tumor
                                                                                                          cells as a result of translocation into the immunoglobu-
                                                                                                          lin heavy-chain locus and is therefore activated in B cell
                                                                                                          malignancies. Bcl-2 expression is seen in 75% of follicular
                                                                                                          lymphomas.




                                                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                                                          dues between BH3 and BH4 of human Bcl-2 was used as
                          Paraffin-embedded human tonsil tissue stained with anti-BCL-2 RabMAb.           immunogen. The antibody does not cross-react with other
                          Note positive staining of follicular cells and negative staining of reactive    Bcl-2 family members
                          cells in the germinal center



                     16    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


Bcl-x                                                                   Cat #: 1018-1      Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:        E18                  Species Reactivity: Human, Mouse, Rat




                                                                                                                                                  Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:    Cytoplasmic               Control:          Hodgkin’s lymphoma,
                                                                                                             Prostatic carcinoma


                                                                           Description: Bcl-x, a member of the Bcl-2 protein family,
                                                                           inhibits cell death, or apoptosis. Bcl-x is expressed as two
                                                                           isomeric forms, Bcl-xL and Bcl-xS, and is typically pres-
                                                                           ent in the cytosol in association with the mitochondrial
                                                                           membrane. Bcl-xL forms heterodimers with various pro-
                                                                           teins, including Bax, Bak and Bcl-2. It has been found that
                                                                           eterodimerization with Bax does not seem to be required for
                                                                           anti-apoptotic activity. Bcl-xL is expressed in Reed Stern-
                                                                           berg cells in 86% of Hodgkin’s cases. It is also expressed in
                                                                           various mammalian epithelial cells in prostate and gastro-
                                                                           intestinal.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues between BH3 and BH3 of Human Bcl-x was used as
Paraffin-embedded human prostatic carcinoma tissue stained with anti-      the immunogen. common to them.
BCL-X RabMAb Note positive staining of tumor cells




Beta Catenin                                                            Cat #: 1247-1      Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:        E247                 Species Reactivity: Human, Mouse

IHC Dilution:   1:100 - 250                     Localization:    Cytoplasmic/              Control:          Breast carcinoma,
                                                                 Cell membrane                               Colon carcinoma


                                                                           Description: Beta Catenin is a key regulatory protein
                                                                           involved in cell adhesion and signal transduction through
                                                                           the Wnt pathway, and plays important roles in develop-
                                                                           ment, cellular proliferation, and differentiation. Beta Catenin
                                                                           is phosphorylated at four serine and threonine residues
                                                                           at the N-terminus. Ser 45 is phosphorylated by CK1, and
                                                                           is thought to be a priming site for the phosphorylation by
                                                                           glycogen synthase kinase-3 (GSK-3) of the remaining three
                                                                           residues, Thr 41, Ser 37, and Ser 33. Overexpression of
                                                                           Beta-catenin has been implicated in metastatic develope-
                                                                           ment in many types of tumors.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues near N-terminus of human Beta Catenin was used
Paraffin-embedded human colonic adenocarcinoma tissue stained with         as immunogen.
anti-Beta Catenin RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                       www.epitomics.com                     info@epitomics.com                         17
                                                                    info@biomol.de                0800-2466651 www.biomol.de


                          Beta Tubulin class III                                                 Cat #: 1967-1      Size: 100ul         min. 50 slides
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                             Type:        Rabbit Monoclonal IgG               Clone:        EP1569Y           Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic             Control:         Brain
Primary Antibodies




                                                                                                    Description: Beta Tubulin class III (beta III-tubulin) is a
                                                                                                    vertebrate tubulin Isotype specific to the neurons and
                                                                                                    mammalian testis cells, making it an ideal neuronal marker.
                                                                                                    Overexpression of class III beta tubulin is associated with
                                                                                                    the resistances of microtubule-targeted cancer drugs in
                                                                                                    lung cancer cell lines, breast cancer cell lines, and ovarian
                                                                                                    tumors.




                                                                                                    Immunogen: A synthetic peptide corresponding to the
                                                                                                    C-term of human Beta Tubulin class III was used as im-
                          Paraffin-embedded human brain tissue stained with anti-Beta-Tubulin-      munogen.
                          Class 3 RabMAb. Note positive staining of neurons




                          Bim                                                                    Cat #: 1036-1      Size: 100ul         min. 50 slides
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                             Type:        Rabbit Monoclonal IgG               Clone:        Y36               Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic             Control:         Breast carcinoma



                                                                                                    Description: Bim, Bcl-2 interacting mediator of cell death, is
                                                                                                    a pro-apoptotic protein belonging to the BH3-only group of
                                                                                                    Bcl-2 family members. Bim promotes apoptosis by binding
                                                                                                    to and inhibiting the activity of anti-apoptotic Bcl-2 family
                                                                                                    members, inducing the release of cytochrome c from mito-
                                                                                                    chondria. Bim is sequestered in an inactive conformation
                                                                                                    through binding to the microtubule-associated dynein motor
                                                                                                    complex. Certain apoptotic stimuli release Bim from micro-
                                                                                                    tubules, allowing inhibitory binding to anti-apoptotic Bcl-2
                                                                                                    family members and subsequent iniation of apoptosis.




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues in the exons 2 of human Bim was used as immuno-
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue       gen.
                          stained with anti-Bim RabMAb. Note positive staining of tumor cell




                     18    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                        info@biomol.de                 0800-2466651 www.biomol.de


Calcitonin                                                         Cat #: 4208-1        Size: 100ul          min. 50 slides
                                                                                       Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:        SP17               Species Reactivity: Human, Mouse, Rat




                                                                                                                                               Primary Antibodies
IHC Dilution:   1:200                         Localization:     Cytoplasmic              Control:         Thyroid medullary
                                                                                                          carcinoma


                                                                        Description: Calcitonin (Katacalcin) is a 32 amino acids
                                                                        poly-peptide hormone that is commonly expressed in
                                                                        the parafollicular or C cells in the thyroid gland. It is also
                                                                        synthesized in a wide variety of other tissues, including
                                                                        the lung and intestinal tract. Calcitonin is a potent plasma
                                                                        calcium-lowering peptide; it decreases the level of calcium
                                                                        and phosphate in blood by promoting the incorporation of
                                                                        those ions in the bones. Calcitonin staining is used in the
                                                                        identification of C cell proliferative abnormalities, such as C
                                                                        cell hyperplasia and invasive tumors. Staining for calcitonin
                                                                        may be used for the identification of a spectrum of C cell
                                                                        proliferative abnormalities ranging from C cell hyperplasia
                                                                        to invasive tumors (e.g. medullary thyroid carcinomas).
                                                                        Calcitonin activitiy might be exhibited in amyloid deposits
                                                                        within the tumor.

                                                                        Immunogen: A recombinant full length mouse calcitonin
                                                                        protein.
Paraffin-embedded human medullary carcinoma of thyroid gland stained
with anti-Calcitonin RabMAb. Note positive staining of tumor cells




Caldesmon                                                          Cat #: 1089-1        Size: 100ul          min. 50 slides
                                                                                       Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:        E89                Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 250                   Localization:     Cytoplasmic              Control:         Smooth muscles



                                                                        Description: Caldesmon is a smooth muscle and non-
                                                                        muscle regulatory protein that interacts with actin, myosin,
                                                                        tropomyosin, and calmodulin. Smooth muscle caldesmon is
                                                                        an elongated molecule with a calmodulin, tropomyosin, and
                                                                        actin-binding region at the C-terminus and a myosin-binding
                                                                        domain at the N-terminus. Caldesmon stimulates actin bind-
                                                                        ing of tropomyosin which increases the stabilization of actin
                                                                        filament structure. In muscle tissues, caldesmon inhibits the
                                                                        actomyosin ATPase by binding to F-actin. This inhibition is
                                                                        reduced by calcium-calmodulin and is promoted by tropo-
                                                                        myosin.




                                                                        Immunogen: A synthetic phospho-peptide correspond-
                                                                        ing to residues surrounding Ser789 of human Caldesmon
Paraffin-embedded human uterine myometrium tissue stained with anti-    was used as immunogen.
Caldesmon RabMAb. Note positive staining of smooth muscles




 Toll Free : 1-877-7RABMAB (772-2622)                      www.epitomics.com                    info@epitomics.com                        19
                                                                   info@biomol.de                0800-2466651 www.biomol.de


                          Calponin                                                             Cat #: 1806-1      Size: 100ul         min. 50 slides
                                                                                                                 Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:        EP798Y             Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                   Localization:     Cytoplasmic             Control:          Smooth muscles
Primary Antibodies




                                                                                                  Description: Calponin is a smooth muscle specific, actin-,
                                                                                                  tropomyosin- and calmodulin-binding protein thought to be
                                                                                                  involved in regulation of actomyosin as well as the regula-
                                                                                                  tion or modulation of contraction. Calponin binds filamen-
                                                                                                  tous actin (F-actin) through two distinct sites ABS1 and
                                                                                                  ABS2, with an affinity in the low micromolar range. Immu-
                                                                                                  noreactivity for calponin, along with alpha-smooth muscle
                                                                                                  actin and smooth muscle myosin heavy chains, confirms
                                                                                                  the known neoplastic myoepithelial component of adenoid
                                                                                                  cystic carcinomas and epithelial-myoepithelial carcinomas.
                                                                                                  The consistently positive staining pattern in adenoid cystic
                                                                                                  carcinomas may be diagnostically useful in discriminating
                                                                                                  histologically similar but consistently negative polymor-
                                                                                                  phous low-grade adenocarcinomas.


                                                                                                  Immunogen: A synthetic peptide corresponding to resi-
                                                                                                  dues near the C-term of human Calponin was used as an
                          Paraffin-embedded human uterine myometrium tissue stained with anti-    immunogen.
                          Calponin RabMAb. Note positive staining of smooth muscles




                          Calretinin                                                           Cat #: 2624-1      Size: 100ul         min. 50 slides
                                                                                                                 Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:        EPR1798            Species Reactivity: Human

                          IHC Dilution:   1:250 - 500                   Localization:     Cytoplasmic             Control:          Mesothelioma



                                                                                                  Description: Calretinin (CAB29, calbindin 2) is a cal-
                                                                                                  cium binding protein that belongs to calbindin family. It is
                                                                                                  mainly found in neuronal tissue, high expressed in audi-
                                                                                                  tory neurons. It has been implicated in as a calcium buffer,
                                                                                                  calcium sensor, and as a regulator of apoptosis. Calretinin
                                                                                                  is considered as a specific marker of mesothelllal cells; it is
                                                                                                  the only marker that has the utility in distinguishing between
                                                                                                  sarcomatoid mesotheliomas and sarcomatoid renal cell
                                                                                                  carcinomas. It is widely used in the panel of makers for
                                                                                                  distinguishing mesothlioma from adenocarcinoma. Along
                                                                                                  with calretinin, the panel includes E-cadherin, CEA, and
                                                                                                  cytokeratin 5/6.




                                                                                                  Immunogen: A synthetic peptide corresponding to resi-
                                                                                                  dues in human Calretinin was used as an immunogen.
                          Paraffin-embedded human mesothelioma tissue stained with anti-Cal-
                          retinin RabMAb. Note positive staining of tumor cells




                     20    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                  0800-2466651 www.biomol.de


Cathepsin D                                                              Cat #: 2487-1      Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EPR3057Y           Species Reactivity: Human, Mouse




                                                                                                                                                  Primary Antibodies
IHC Dilution:   1:500 - 1000                    Localization:      Cytoplasmic              Control:         Liver,
                                                                                                             Breast carcinoma


                                                                            Description: Cathepsin D is a ubiquitously expressed
                                                                            lysosomal protease that is involved in proteolytic degrada-
                                                                            tion, cell invasion, and apoptosis. It is suspected to play
                                                                            important roles in protein catabolism, antigen processing,
                                                                            degenerative diseases, and breast cancer progression.
                                                                            Cathepsin D is induced by estrogens in mammary cancer
                                                                            cells where its concentration is correlated with a higher risk
                                                                            of metastasis. It is possibly involved in Alzheimer’s disease-
                                                                            related neurodegeneration through cleavage of amyloid
                                                                            precursor protein into amyloidogenic components.




                                                                            Immunogen: A synthetic peptide corresponding to resi-
                                                                            dues on the C-terminus of human Cathepsin D was used
Paraffin-embedded human breast ductal infiltrating carcinoma tissue         as an immunogen.
stainedd with anti-Cathepsin D (precursor and mature) RabMAb. Note
positive staining of tumor cells




Cathepsin D (pro)                                                        Cat #: 2510-1      Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EPR3054Y           Species Reactivity: Human

IHC Dilution:   1:250 - 500                     Localization:      Cytoplasmic              Control:         Liver,
                                                                                                             Breast carcinoma


                                                                            Description: Cathepsin D is a ubiquitously expressed
                                                                            lysosomal protease that is involved in proteolytic degrada-
                                                                            tion, cell invasion, and apoptosis. It is suspected to play
                                                                            important roles in protein catabolism, antigen processing,
                                                                            degenerative diseases, and breast cancer progression.
                                                                            Cathepsin D is induced by estrogens in mammary cancer
                                                                            cells where its concentration is correlated with a higher risk
                                                                            of metastasis. It is possibly involved in Alzheimer’s disease-
                                                                            related neurodegeneration through cleavage of amyloid
                                                                            precursor protein into amyloidogenic components.




                                                                            Immunogen: A synthetic peptide corresponding to resi-
                                                                            dues in human Cathepsin D was used as an immunogen.
Paraffin-embedded human breast ductal infiltrating carcinoma tissue
stained with anti-Cathepsin D (precursor) RabMAb. Note positive stain-
ing of tumor cells



 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                     info@epitomics.com                        21
                                                                   info@biomol.de                0800-2466651 www.biomol.de


                          Caveolin-1                                                          Cat #: 1249-1        Size: 100ul          min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:          E249             Species Reactivity: Human, Mouse

                          IHC Dilution:   1:100 - 250                    Localization:      Cytoplasmic            Control:          Urinary bladder,
Primary Antibodies




                                                                                                                                     Lung


                                                                                                   Description: Caveolins make up a family of proteins that
                                                                                                   are principal structural components of hairpin-like domains
                                                                                                   in the plasma membrane. It is believed that caveolins serve
                                                                                                   as scaffolding proteins for the integration of signal transduc-
                                                                                                   tion. Three members of caveolins (caveolin-1, -2 and -3)
                                                                                                   have been identified, possessing different tissue distribu-
                                                                                                   tions. Caveolins interact with multiple signaling molecules,
                                                                                                   such as the G-protein alpha subunit, tyrosine kinase recep-
                                                                                                   tors, PKCs, Src family tyrosine kinases and eNOS. Caveo-
                                                                                                   lin-1 has been implicated in the pathogenesis of mammary
                                                                                                   epithelial cell hyperplasia.




                                                                                                   Immunogen: A synthetic peptide corresponding to resi-
                                                                                                   dues near C-terminus of human Caveolin-1 was used as
                          Paraffin-embedded human urinary bladder muscular tissue stained with     immunogen. The antibody should recognize both alpha
                          anti-Caveolin-1 RabMAb. Note positive staining of smooth muscles         and beta form of Caveolin-1.




                          CD3 epsilon                                                         Cat #: 1774-1        Size: 100ul          min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:          EP449E           Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                    Localization:      Cell membrane          Control:          Tonsil,
                                                                                                                                     Lymph node


                                                                                                   Description: CD3 (Cluster of Differentiation 3) is a complex
                                                                                                   of proteins that associates directly with the T cell antigen
                                                                                                   receptor (TCR). Antigen binding to the TCR leads to IL-2
                                                                                                   secretion via activation of a tyrosine phosphorylation path-
                                                                                                   way and a phospholipase C (PLC) pathway, in turn activat-
                                                                                                   ing protein kinase C. CD3 is composed of five invariant
                                                                                                   polypeptide chains that associate to form three dimers. The
                                                                                                   five invariant chains of CD3 are labeled gamma, delta, ep-
                                                                                                   silon, zeta, and eta. The gamma, epsilon and delta chains
                                                                                                   each contain a single copy of a conserved immunorecep-
                                                                                                   tor tyrosine-based activation motif (ITAM). Phosphorylated
                                                                                                   ITAMs act as docking sites for protein kinases such as
                                                                                                   ZAP-70 and Syk.




                                                                                                   Immunogen: A synthetic peptide corresponding to resi-
                                                                                                   dues in cytoplasmic domain of human CD3 epsilon was
                          Paraffin-embedded human lymph node tissue stained with anti-CD3          used as immunogen. The antibody does not cross-react
                          epsilon RabMAb. Note positive staining of T lymphocytes                  with other subunits of CD3.




                     22    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                        info@biomol.de                0800-2466651 www.biomol.de


CD5                                                                 Cat #: 2541-1      Size: 100ul          min. 50 slides
                                                                                      Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:           EP2952          Species Reactivity: Human




                                                                                                                                             Primary Antibodies
IHC Dilution:   1:250 - 500                   Localization:       Cell Membrane         Control:         Tonsil



                                                                        Description: CD5 (Lymphocyte antigen T1/Leu-1) is a
                                                                        transmembrane glycoprotein which has been implicated as
                                                                        a receptor in the regulation of T-cell proliferation. CD5 is
                                                                        part of the scavenger receptor cysteine-rich (SRCR) family
                                                                        group B and it is found on 95% of thymocytes and 72% of
                                                                        peripheral blood lymphocytes. In lymph nodes, the main
                                                                        reactivity is observed in T cell areas. CD5 is expressed by
                                                                        T cell leukemia, lymphomas, and activated T cells. Occa-
                                                                        sionally, CD5 antigen is also expressed on a subset of B
                                                                        cells. Mantle cell lymphomas (same as diffuse centrocytic
                                                                        lymphomas) are CD5+ while the follicle center cell lympho-
                                                                        mas are CD5..




                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                        dues in human CD5 was used as an immunogen.
Paraffin-embedded human tonsil tissue stained with anti-CD5 RabMAb.
Note positive staining of T lymphocytes




CD8                                                                 Cat #: 2519-1      Size: 100ul          min. 50 slides
                                                                                      Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:           EPR1150         Species Reactivity: Human

IHC Dilution:   1:250 - 500                   Localization:       Cell Membrane         Control:         Tonsil



                                                                        Description: The T cell coreceptor CD8 is a cell-surface gly-
                                                                        coprotein expressed either as a disulfide-linked homodimer
                                                                        of two CD8alpha monomers, or a heterodimer of CD8alpha
                                                                        and CD8beta. These receptors interact with ligands, such
                                                                        as major histocompatibility complex (MHC) class I, on the
                                                                        outside of the cell, with proteins inside the cell, such as
                                                                        the tyrosine kinase p56lck, and possibly with proteins on
                                                                        the same cell-surface. CD8 plays an important role in T
                                                                        cell development in the thymus and T cell activation in the
                                                                        periphery.




                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                        dues near the C-terminus of human CD8 was used as an
Paraffin-embedded human lymph node tissue stained with anti-CD8         immunogen.
RabMAb. Note positive staining of T lymphocytes




 Toll Free : 1-877-7RABMAB (772-2622)                     www.epitomics.com                    info@epitomics.com                       23
                                                                  info@biomol.de                 0800-2466651 www.biomol.de


                          CD11c                                                             Cat #: 1975-1        Size: 100ul          min. 50 slides
                                                                                                                Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG            Clone:        EP1347Y            Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                   Localization:    Cell Membrane           Control:          Tonsil
Primary Antibodies




                                                                                                  Description: Leukocyte surface antigen p150,95 (CD11c,
                                                                                                  ITAX) shares a beta subunit with 2 other members of the
                                                                                                  leukocyte adhesion molecule family, Mac-1 and LFA-1, but
                                                                                                  has a unique alpha chain. The alpha subunit of the leuko-
                                                                                                  cyte integrin p150,95 (CD11c) is a type 1 transmembrane
                                                                                                  polypeptide. CD11c is highly expressed in human dendritic
                                                                                                  cells, and is also expressed in monocytes, macrophages,
                                                                                                  neutrophils, and some B cells. It is commoly expressed in
                                                                                                  various leukemias (e.g. hairy cell leukemias, acute nonlym-
                                                                                                  phocytic leukemias, and some B-cell chronic lymphocytic
                                                                                                  leukemias).




                                                                                                  Immunogen: A synthetic peptide corresponding to resi-
                                                                                                  dues on the C-terminus of human CD11c was used as an
                          Paraffin-embedded human lymph node using anti-CD11c RabMAb. Note        immunogen.
                          positive staining of granulocytes and monocytes




                          CD14                                                              Cat #: 2641-1        Size: 100ul          min. 50 slides
                                                                                                                Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG            Clone:        EPR3653            Species Reactivity: Human

                          IHC Dilution:   1:250 - 500                   Localization:    Cell Membrane           Control:          Tonsil



                                                                                                  Description: CD14 is a single copy gene encoding 2 protein
                                                                                                  forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored
                                                                                                  membrane protein (mCD14) and a monocyte or liver de-
                                                                                                  rived soluble serum protein (sCD14) that lacks the anchor.
                                                                                                  Both molecules are critical for lipopolysaccharide (LPS)
                                                                                                  dependent signal transduction, and sCD14 confers LPS
                                                                                                  sensitivity to cells lacking mCD14. Increased sCD14 levels
                                                                                                  are associated with inflammatory infectious diseases and
                                                                                                  high mortality in gram negative shock.




                                                                                                  Immunogen: A synthetic peptide corresponding to resi-
                                                                                                  dues in human CD14 was used as an immunogen.
                          Paraffin-embedded human tonsil tissue stained with anti-CD14 RabMAb.
                          Note positive staining of follicular dendritic cells




                     24    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                        info@biomol.de                 0800-2466651 www.biomol.de


CD20                                                              Cat #: 1632-1         Size: 100ul          min. 50 slides
                                                                                       Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:        EP459Y              Species Reactivity: Human, Mouse




                                                                                                                                               Primary Antibodies
IHC Dilution:   1:100 - 250                   Localization:    Cell Membrane             Control:         Tonsil



                                                                        Description: CD20, also known as B1 and MS4A1, is a
                                                                        human B lymphocyte surface molecule expressed on both
                                                                        normal and malignant cells that functions as a receptor
                                                                        during B cell activation. It is also involved in the regulation
                                                                        of cell progression in B lymphocytes as either a compo-
                                                                        nent or a regulator of intracellular calcium levels. CD20 is
                                                                        phosphorylated on serines primarily and threonines but not
                                                                        tyrosines. Monoclonal antibodies against CD20 are used to
                                                                        treat B-cell non-Hodgkins lymphoma.




                                                                        Immunogen: A synthetic peptide corresponding to
                                                                        residues in the C-terminus of human CD20 was used as
Paraffin-embedded human tonsil tissue stained with anti-CD20 RabMAb.    immunogen.
Note positive staining of B lymphocytes




CD21                                                              Cat #: 2546-1         Size: 100ul          min. 50 slides
                                                                                       Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:        EP3093              Species Reactivity: Human

IHC Dilution:   1:250 - 500                   Localization:    Cell Membrane             Control:         Tonsil



                                                                        Description: CD21 (complement receptor 2, C3d receptor,
                                                                        EBV receptor) is a 140 kDa glycosylated type I transmem-
                                                                        brane protein. CD21 serves as the complement receptor
                                                                        for C3d and the Epstein-Barr virus. CD21 has been linked
                                                                        to inflammatory disorders, due to its ability to induce the
                                                                        release of proinflammatory cytokines. CD21 is expressed
                                                                        in B cell chronic lymphocytic leukaemias and in a subset of
                                                                        T cell acute lymphocytic leukaemias. CD21 is also ex-
                                                                        pressed in follicular and centrocytic lymphomas, and mature
                                                                        leukaemias.




                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                        dues on the C-terminus of human CD21 was used as an
Paraffin-embedded human tonsil tissue stained with anti-CD21 RabMAb.    immunogen.
Note positive staining of B lymphocytes




 Toll Free : 1-877-7RABMAB (772-2622)                     www.epitomics.com                     info@epitomics.com                        25
                                                                  info@biomol.de                 0800-2466651 www.biomol.de


                          CD29/Integrin beta-1                                                   Cat #: 2288-1      Size: 100ul          min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:           EPR1040Y        Species Reactivity: Human

                          IHC Dilution:   1:250                          Localization:        Cell Membrane         Control:          Tonsil,
Primary Antibodies




                                                                                                                                      Brain


                                                                                                    Description: CD29/Integrin beta-1 is the beta subunit
                                                                                                    found in the integrin families, forming a heterodimer inte-
                                                                                                    grin receptor through non-covalent bonding with various
                                                                                                    integrin alpha subunits. Integrin heterodimer containing
                                                                                                    CD/29Integrin beta-1 binds to various cell surface and
                                                                                                    extracellular proteins (CD49a-f, CD51) to mediate cell to
                                                                                                    cell and cell to matrix adhesion. CD29/Integrin beta-1 plays
                                                                                                    a critical role in the cell adhesion and recognition in em-
                                                                                                    bryogenesis, hemostasis, immune response, tissue repair,
                                                                                                    metastatic diffusion of tumor cells and development.




                                                                                                    Immunogen: A synthetic peptide corresponding to the
                                                                                                    residues in human CD29/Intergrin beta-1 was used as
                          Paraffin-embedded human brain tissue stained with anti-CD29/Integrin      immunogen.
                          beta-1 RabMAb. Note positive staining of endothelial cells




                          CD31                                                                   Cat #: 2540-1      Size: 100ul          min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:           EPR3094         Species Reactivity: Human

                          IHC Dilution:   1:250 - 500                    Localization:        Cell Membrane         Control:          Tonsil,
                                                                                                                                      Muscle


                                                                                                    Description: Platelet endothelial cell adhesion molecule-1
                                                                                                    (CD31/PECAM-1) is a cell-cell adhesion molecule that is
                                                                                                    expressed on circulating platelets, on leukocytes, and at the
                                                                                                    intercellular junctions of vascular endothelial cells, where it
                                                                                                    mediates the interactions of these cells during the process
                                                                                                    of transendothelial cell migration, thrombosis, wound heal-
                                                                                                    ing, and angiogenesis. CD31/PECAM-1 is an important
                                                                                                    target glycoprotein in drug-induced immune thrombocy-
                                                                                                    topenia (DITP), a serious complication of drug treatment.
                                                                                                    CD31/PECAM-1 is the physiological ligand for CD38, where
                                                                                                    expression in a high percentage of malignant cells is a risk
                                                                                                    factor for patients with B-cell chronic lymphocytic leukaemia
                                                                                                    (B-CLL).




                                                                                                    Immunogen: A synthetic peptide corresponding to
                                                                                                    residues in the extracellular domain of human CD31 was
                          Paraffin-embedded human skeletal muscle tissue stained with anti-         used as an immunogen.
                          CD31/PECAM-1 RabMAb. Note positive staining of endothelial cells




                     26    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                   0800-2466651 www.biomol.de


CD34                                                                 Cat #: 2150-1          Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:        EP373Y              Species Reactivity: Human




                                                                                                                                                  Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:     Cell Membrane             Control:         Tonsil



                                                                            Description: CD34 (Cluster of differentiation 34) is a single-
                                                                            pass type I transmembrane glycoprotein which primary
                                                                            functions as a cell to cell adhesion factor. As an adhesion
                                                                            factor, CD34 is expressed during stem/progenitor stage
                                                                            of lymphohematopoietic development and possibly medi-
                                                                            ates the stem cell attachment to the bone marrow ECM or
                                                                            stromal cells. CD34 is considered to be ideal marker for
                                                                            identifying and quantifying hematopoietic progenitor stem
                                                                            cells. With interaction with CrkL, CD34 becomes a substrate
                                                                            for PKC and CD34 surface expression is associated with
                                                                            activation of PKC.




                                                                            Immunogen: A synthetic peptide corresponding to C-
                                                                            terminal of human CD34 was used as an immunogen.
Paraffin-embedded human tonsil tissue stained with anti-CD34 RabMAb.
Note positive staining of vascular endothelium




CD44                                                                 Cat #: 1998-1          Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:        EPR1013Y            Species Reactivity: Human

IHC Dilution:   1:100 - 250                     Localization:     Cell Membrane             Control:         Tonsil,
                                                                                                             Transitional cell carcinoma


                                                                            Description: CD44 is a cell-surface glycoprotein postulated
                                                                            to play a role in a variety of biological processes, includ-
                                                                            ing lymphocyte homing and tumor-cell metastasis. Several
                                                                            isoforms of CD44 have been identified in human cells, and
                                                                            the genesis of some of these isoforms has been attributed
                                                                            to alternative splicing. Expression of CD44 splice variants
                                                                            has been linked to metastasis and poor prognosis in certain
                                                                            cancer.




                                                                            Immunogen: A synthetic peptide corresponding to resi-
                                                                            dues in human CD44 was used as an immunogen.
Paraffin-embedded human urinary bladder transitional cell carcinoma tis-
sue stained with anti-CD44 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                     info@epitomics.com                        27
                                                                    info@biomol.de                 0800-2466651 www.biomol.de


                          CD56                                                                    Cat #: 2433-1      Size: 100ul          min. 50 slides
                                                                                                                    Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EP2567Y            Species Reactivity: Human

                          IHC Dilution:   1:500 - 2000                    Localization:     Cell Membrane            Control:          Astrocytoma
Primary Antibodies




                                                                                                     Description: The neural cell adhesion molecule (NCAM/
                                                                                                     CD56) has been shown to mediate homophilic cell-cell
                                                                                                     adhesion in the neuroendocrine system with interactions
                                                                                                     among immunoglobulin-like domains. It is a multifunction
                                                                                                     transmembrane protein involved in synaptic plasticity, neu-
                                                                                                     rodevelopment, and neurogenesis. NCAM appears on early
                                                                                                     embryonic cells and is important in the formation of cell col-
                                                                                                     lectives and their boundaries at sites of morphogenesis. It is
                                                                                                     preferentially expressed on human NK cells and a subset of
                                                                                                     T lymphocytes that mediate MHC-unrestricted cell-mediated
                                                                                                     cytotoxicity.




                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near the C-terminus of human CD56/NCAM was
                          Paraffin-embedded human astrocytoma using anti-CD56/NCAM                   used as an immunogen.
                          RabMAb. Note positive staining of tumor glial cells




                          CD61                                                                    Cat #: 2264-1      Size: 100ul          min. 50 slides
                                                                                                                    Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EP2417Y            Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100                           Localization:     Cell Membrane            Control:          Tonsil



                                                                                                     Description: Platelet membrane glycoprotein CD61/Integrin
                                                                                                     beta-3 (GP IIIa) forms a Ca2+-dependent heterodimer com-
                                                                                                     plex with GP IIb. The GP IIb-IIIa complex constitutes the
                                                                                                     fibrinogen and fibronectin receptor on stimulated platelets.
                                                                                                     A biochemically and immunologically similar membrane gly-
                                                                                                     coprotein complex is present on endothelial cells. Homology
                                                                                                     suggests that GP IIIa is a member of a family of cell-surface
                                                                                                     adhesion receptors. Data suggest that tyrosine phosphory-
                                                                                                     lation of an integrin beta subunit may be important in initiat-
                                                                                                     ing outside-in signaling cascades by inducing association of
                                                                                                     signaling components directly with the integrin.




                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near C-terminus (the cytoplasmic region) of human
                          Paraffin-embedded human tonsil tissue stained with anti-CD61/Integrin      CD61/Integrin beta-3 was used as an immunogen.
                          beta-3 RabMAb. Note positive staining of endothelial cells




                     28    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                         info@biomol.de              0800-2466651 www.biomol.de


CD79a                                                               Cat #: 2613-1      Size: 100ul         min. 50 slides
                                                                                     Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:        EPR3618          Species Reactivity: Human




                                                                                                                                            Primary Antibodies
IHC Dilution:   1:250 - 500                    Localization:    Cell Membrane          Control:         Tonsil



                                                                       Description: CD79a (IGBP1, Alpha 4 protein) initially
                                                                       expressed at pre B cell stage and persists until the plasma
                                                                       cell stage where it is found as an intracellular component.
                                                                       CD79a associates with surface IgM-receptors; it has been
                                                                       implicated in the signal transduction pathways and in the
                                                                       regulation of type of type 2A-related serine/threonine phos-
                                                                       phatases catalytic activity. A disulphide-linked heterodimer,
                                                                       consisting of CD79a / mb-1 and CD79b / B29 polypeptides,
                                                                       non-covalently associates with membrane-bound immu-
                                                                       noglobulins on B cells to constitute the B cell Ag recep-
                                                                       tor. CD79a is found in the majority of acute leukemias of
                                                                       precursor B cell type, in B cell lines, B cell lymphomas, and
                                                                       in some myelomas.




                                                                       Immunogen: A synthetic peptide corresponding to resi-
                                                                       dues in human CD79a was used as an immunogen.
Paraffin-embedded human tonsil tissue stained with anti-CD79a
RabMAb. Note positive staining of B lymphocytes




CD99                                                                Cat #: 2429-1      Size: 100ul         min. 50 slides
                                                                                     Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:        EPR3097Y         Species Reactivity: Human

IHC Dilution:   1:1000 - 2000                  Localization:    Cell Membrane          Control:         Ewing’s sarcoma,
                                                                                                        Pancreas


                                                                       Description: CD99, or protein MIC2, is a T-cell surface
                                                                       glycoprotein involved in the T-cell adhesion process and
                                                                       spontaneous rosette formation with erythrocytes. The cell
                                                                       surface antigen defined by the monoclonal antibody CD99
                                                                       is expressed by both the human X and Y chromosomes; the
                                                                       gene loci on the X and Y chromosomes are referred to as
                                                                       MIC2X and MIC2Y, respectively. It was demonstrated that
                                                                       engagement of distinct epitopes on CD99 rapidly induces T
                                                                       cell death by a novel caspase-independent pathway. Thus,
                                                                       it is suggested that CD99 is linked to a novel death pathway
                                                                       that may have biologic relevance in control of early T cells.
                                                                       CD99 is highly expressed in Ewing’s sarcoma cells, pancre-
                                                                       atic cells, and neuroectodermal tumors.




                                                                       Immunogen: A synthetic peptide corresponding to resi-
                                                                       dues on the C-terminus of human CD99 was used as and
Paraffin-embedded human Ewing’s sarcoma tissue stained with anti-      immunogen.
CD99 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                       www.epitomics.com                 info@epitomics.com                       29
                                                                    info@biomol.de                0800-2466651 www.biomol.de


                          CD146                                                                  Cat #: 2505-1        Size: 100ul        min. 50 slides
                                                                                                                    Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EPR3208             Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:250 - 500                     Localization:     Cell Membrane             Control:         Melanoma
Primary Antibodies




                                                                                                      Description: CD146 (melanoma-associated antigen A32
                                                                                                      or MUC18) is a transmembrane glycoprotein expressed on
                                                                                                      endothelial cells and is located at the intercellular junction
                                                                                                      where it plays a role in cell adhesion, and in the cohesion of
                                                                                                      the endothelial monolayer. CD146 engagement initiates an
                                                                                                      outside-in signaling pathway involving the protein tyrosine
                                                                                                      kinases FYN and FAK as well as Paxillin. In addition, it is a
                                                                                                      signaling molecule involved in the dynamics of actin cyto-
                                                                                                      skeleton rearrangement . CD146 is associated with tumor
                                                                                                      progression and the development of metastasis in human
                                                                                                      malignant melanoma. Expression of the antigen is restricted
                                                                                                      to advanced primary and metastatic melanomas and to cell
                                                                                                      lines of the neuroectodermal lineage.




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues in human CD146 was used as an immunogen.
                          Paraffin-embedded human melanoma tissue stained with anti-CD146
                          RabMAb. Note positive staining of tumor cells




                          CDK9                                                                   Cat #: 2454-1        Size: 100ul        min. 50 slides
                                                                                                                    Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EPR3119Y            Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                     Localization:     Nuclear                   Control:         Cervical carcinoma,
                                                                                                                                       Breast carcinoma


                                                                                                      Description: CDK9 (cyclin-dependent kinase 9, serine/
                                                                                                      threonine-protein kinase PITALRE) is a member of the
                                                                                                      cell division cycle 2 (CDC2) family of kinases that play a
                                                                                                      pivotal role in the regulation of the eukaryotic cell cycle.
                                                                                                      CDK9 expression is ubiquitous, but its expression levels are
                                                                                                      different in various human tissues. At pathophysiological
                                                                                                      levels, CDK9 activity suppresses many genes for mitochon-
                                                                                                      drial proteins including master regulators of mitochondrial
                                                                                                      function (peroxisome proliferator-activated receptor gamma
                                                                                                      coactivator 1 (PGC-1), nuclear respiratory factor-1).




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues on the C-terminus of human CDK9 was used as an
                          Paraffin-embedded human breast ductal infiltrating carcinoma stained      immunogen.
                          with anti-CDK9 RabMAb. Note positive staining of tumor cells




                     30    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                  0800-2466651 www.biomol.de


CDX2                                                                    Cat #: 2475-1           Size: 100ul          min. 50 slides
                                                                                               Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:            EPR2764Y             Species Reactivity: Human




                                                                                                                                                         Primary Antibodies
IHC Dilution:   1:250 - 500                      Localization:        Nuclear                    Control:         Colorectal carcinoma



                                                                                Description: The caudal-related homeodomain protein 2,
                                                                                CDX2, is a transcription factor which is expressed in the
                                                                                intestine and is thought to play an important role in the
                                                                                proliferation and differentiation of intestinal epithelial cells.
                                                                                The CDX2 protein is expressed in primary and metastatic
                                                                                colorectal carcinomas, intestinal metaplasia of the stomach
                                                                                and intestinal type gastric cancer. In human colorectal can-
                                                                                cer, the expression of both CDX2 and carbonic anhydrase
                                                                                1, a gene regulated by CDX2, is reduced or absent. CDX2
                                                                                is one of the important regulators in defining pathways for
                                                                                coordinate control of drug metabolism in the gastrointestinal
                                                                                tract .




                                                                            Immunogen: A synthetic peptide corresponding to resi-
                                                                            dues near the N-terminus of human CDX2 was used as
Paraffin-embedded human colonic adenocarcinoma tissue stained with          an immunogen.
anti-CDX2 RabMAb. Note positive staining of tumor cells




Chromogranin A                                                          Cat #: 1809-1           Size: 100ul          min. 50 slides
                                                                                               Inquire for Bulk

   Type:        Rabbot Monoclonal IgG               Clone:            EP1031Y              Species Reactivity: Human

IHC Dilution:   1:100 - 250                      Localization:        Cytoplasmic                Control:         Pancreas



                                                                                Description: Chromogranin A (CgA) is an 86 kDa protein
                                                                                that is the major member of the granin family of acidic se-
                                                                                cretory glycoproteins located in neuroendocrine cells. CgA
                                                                                is believed to play a role in targeting peptide hormones and
                                                                                neurotransmitters to granules of the regulated pathways
                                                                                and inhibit hormone and neurotransmitter release. CgA lev-
                                                                                els which reflect neuroendocrine differentiation of prostatic
                                                                                carcinoma may have a diagnostic, therapeutic and prognos-
                                                                                tic role in the management of prostate cancer patients.




                                                                            Immunogen: A synthetic peptide corresponding to resi-
                                                                            dues near the C-terminus of human Chromogranin A was
Paraffin-embedded human pancreas tissue stained with anti-Chro-             used as an immunogen.
mogranin A RabMAb. Note positive staining of secretory cells in the
Langerhans islets



 Toll Free : 1-877-7RABMAB (772-2622)                         www.epitomics.com                         info@epitomics.com                          31
                                                                   info@biomol.de                   0800-2466651 www.biomol.de


                          c-Kit                                                                Cat #: 1522-1         Size: 100ul         min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         Y145               Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic,             Control:         Gastric stromal tumor
Primary Antibodies




                                                                                            Cell Membrane


                                                                                                     Description: Member of the Tyrosine Kinase Receptor
                                                                                                     (TKRs) and highly homologous to receptor PDF and CSF-1,
                                                                                                     c-Kit (CD117) is a cellular homologue of the oncogene v-Kit
                                                                                                     of the Hardy Zuckerman 4-feline sarcoma virus. Activation
                                                                                                     of c-Kit tyrosine kinase by SCF (Stem Cell factor) leads to
                                                                                                     autophosphorylation and association of c-Kit with substrate
                                                                                                     PI3K. Missense substitution within the cellular tyrosine
                                                                                                     kinase may cause Piebaldism an autosomal dominant
                                                                                                     disorder of the skin. C-Kit is expressed in several lineage of
                                                                                                     stem cells, and its expression is a defining characterstic of
                                                                                                     gastrointestinal stromal tumors (GIST).




                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues in the C-terminus (cytoplasmic domain) of human
                          Paraffin-embedded human gastric stromal tumor tissue stained with anti-    c-Kit was used as an immunogen.
                          CD117c-Kit RabMAb. Note positive staining of tumor cells




                          COX2                                                                 Cat #: 4212-1         Size: 100ul         min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         SP21               Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100                           Localization:     Cytoplasmic,             Control:         Colonic carcinoma
                                                                                            Cell Membrane


                                                                                                     Description: COX2 (Cyclooxygenase-2. PGHS2) is an
                                                                                                     inducible dimeric enzyme belonging to prostaglandin G/H
                                                                                                     synthase family. COX2, similar to COX1, functions to
                                                                                                     form prostaglandins by catalyzing the synthesis of cyclic
                                                                                                     endoperoxides from arachidonic acid. COX2 expression
                                                                                                     is induced by lipopolysaccaride (LPS) and peptoglycan
                                                                                                     (PGN) and is highly expressed in activated marcophages.
                                                                                                     It has been implicated as a mediator of inflammation and a
                                                                                                     possible role in prostanoid signaling in activity-dependent
                                                                                                     plasticity. COX2 is considered as an ideal marker in im-
                                                                                                     mune response regulation studies and a therapeutic target
                                                                                                     against inflammatory diseases. Overexpression of COX2 by
                                                                                                     85-90% is seen human colorectal adenocarcinoma.




                                                                                                     Immunogen: A synthetic peptide corresponding to C-
                                                                                                     terminus of rat COX2.
                          Paraffin-embedded human colonic adenocarcinoma tissue stained with
                          anti-COX2 RabMAb. Note positive staining of tumor cells




                     32    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                           info@biomol.de               0800-2466651 www.biomol.de


Cyclin E2                                                              Cat #: 1775-1         Size: 100ul         min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EP454Y              Species Reactivity: Human




                                                                                                                                                    Primary Antibodies
IHC Dilution:   1:250 - 500                      Localization:     Nuclear                   Control:          Breast carcinoma



                                                                             Description: Cyclin E2 is a member of the cyclin E family
                                                                             that can associate with and activate Cdk2. Expression of
                                                                             cyclin E2 is essential for the control of the cell cycle at the
                                                                             late G1 and early S phase, and this protein may regulate
                                                                             distinct rate-limiting pathway(s) during this phase. Cyclin
                                                                             E2 specifically interacts with Cdk inhibitors of the CIP/KIP
                                                                             family and activates both Cdk2 and Cdk3. Expression of the
                                                                             viral E6 oncoprotein in normal human fibroblasts increases
                                                                             levels of cyclin E2, but not cyclin E1, while expression of the
                                                                             E7 oncoprotein upregulates both. Abnormally high levels
                                                                             of cyclin E expression have frequently been observed in
                                                                             human cancers.




                                                                          Immunogen: A synthetic peptide corresponding to a resi-
                                                                          due of human Cyclin E2 was used as an immunogen.
Paraffin-embedded human breast ductal infiltrating carcinoma tissue
stainedd with anti-Cyclin E2 RabMAb. Note positive staining of tumor
cells




Cytokeratin 4                                                          Cat #: 1994-1         Size: 100ul         min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EP1599Y             Species Reactivity: Human

IHC Dilution:   1:100 - 250                      Localization:     Cytoplasmic               Control:          Cervical squamous cell
                                                                                                               carcinoma


                                                                             Description: Keratins (Cytokeratins/CK) are a family of
                                                                             structurally related proteins that form the intermediate fila-
                                                                             ment cytoskeleton in epithelial cells. Keratins are expressed
                                                                             in pairs by epithelial cells in a tissue and cell specific
                                                                             manner. The major differentiation specific keratins of the
                                                                             buccal mucosa, nasal, esophageal and anogenital epithelia
                                                                             are CK-4 and CK-13. CK-4 belongs to the type B (II) basic
                                                                             subfamily and commonly found in non-cornifying squamous
                                                                             epithelium. CK-4 is expressed in basal cells and it expres-
                                                                             sion is seen in squamouse cell carcinomas.




                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                          dues on the C-terminus of human Cytokeratin 4 was used
Paraffin-embedded human cervical squamous cell carcinoma tissue           as an immunogen.
stained with anti-CK-4 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                         www.epitomics.com                     info@epitomics.com                         33
                                                                     info@biomol.de               0800-2466651 www.biomol.de


                          Cytokeratin 5                                                          Cat #: 1988-1      Size: 100ul         min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         EP1601Y          Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                      Localization:     Cytoplasmic            Control:         Mesothelioma,
Primary Antibodies




                                                                                                                                     Squamous cell cervial
                                                                                                                                     carcinoma

                                                                                                    Description: The 58-kD keratin 5 (Cytokeratin 5, CK-5) is
                                                                                                    highly similar to other type II keratins and less similar to
                                                                                                    type I keratins and other intermediate filament proteins. The
                                                                                                    58-kD keratin is regulated by retinoids in several tissues
                                                                                                    and is one of four keratins abundantly expressed in epider-
                                                                                                    mal keratinocytes, where it may be important in maintain-
                                                                                                    ing structural integrity of the integument. CK-5 mRNA and
                                                                                                    protein are shown to be expressed in normal mammary
                                                                                                    epithelial cells in culture and are absent from tumor-derived
                                                                                                    cell lines. This makes CK-5 an important marker in the
                                                                                                    tumorigenic process, distinguishing normal from tumor cells,
                                                                                                    and decreased CK-5 expression correlates with tumorigenic
                                                                                                    progression. CK-5 is expresed in basal cell epitheliomas,
                                                                                                    varous suamous cell carcinomas (skin, tongue), and variety
                                                                                                    of epithelial and biphasic mesotheliomas.

                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues on the C-terminus of human CK-5 was used as an
                          Paraffin-embedded human cervical squamous cell carcinoma tissue           immunogen.
                          stained with anti-CK-5 RabMAb. Note positive staining of tumor cells




                          Cytokeratin 6                                                          Cat #: 1951-1      Size: 100ul         min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         EPR1603Y         Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                      Localization:     Cytoplasmic            Control:         Squamous cell cervical
                                                                                                                                     carcinoma, Skin


                                                                                                    Description: The human type II Cytokeratin 6 (CK-6; 56
                                                                                                    kDa) is expressed in a heterogeneous array of epithelial
                                                                                                    tissues under normal conditions, but is better known for its
                                                                                                    strong induction in stratified epithelia that feature an en-
                                                                                                    hanced cell proliferation rate or abnormal differentiation. It
                                                                                                    has been demonstrated that CK-6 isoform, CK-6a, is clearly
                                                                                                    the dominant CK-6 isoform in skin tissue samples and cul-
                                                                                                    tured epithelial cell lines and that the various isoforms are
                                                                                                    differentially regulated within and between epithelial tissue
                                                                                                    types. CK-6 is expressed in approximately 76 percent of
                                                                                                    head and neck squamous cell carcinoma cases.




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues on the C-terminus of human CK-6A was used as an
                          Paraffin-embedded human cervical squamous cell carcinoma tissue           immunogen.
                          stained with anti-CK-6 RabMAb. Note positive staining of tumor cells




                     34    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                           info@biomol.de                  0800-2466651 www.biomol.de


Cytokeratin 7                                                             Cat #: 1899-1      Size: 100ul         min. 50 slides
                                                                                           Inquire for Bulk

   Type:        Rabbit Polyclonal IgG                Clone:         Polyclonal         Species Reactivity: Human
                Affinity Purified




                                                                                                                                                  Primary Antibodies
IHC Dilution:   1:100 - 250                       Localization:     Cytoplasmic              Control:         Transitional cell carcinoma,
                                                                                                              Breast carcinoma

                                                                             Description: Keratins are cytoplasmic intermediate filament
                                                                             proteins expressed by epithelial cells. Cytokeratin 7 (CK-7)
                                                                             is a basic cytokeratin which is expressed in a wide range of
                                                                             epithelial structures in human. The pattern of immunohis-
                                                                             tochemical expression of CK-7 along with CK-20 is com-
                                                                             monly used to assess possible primary sites of metastatic
                                                                             carcinomas. CK-7 expression is found in breast and ovary
                                                                             adenocarcinomas, bladder carcinomas, and chromophobe
                                                                             renal cell carcinoma.




                                                                             Immunogen: A synthetic peptide corresponding to resi-
                                                                             dues near the N-terminus of human CK-7 was used as an
Paraffin-embedded human urinary bladder transitional cell carcinoma          immunogen.
tissue stained with anti-CK-7 Rabbit Polyclonal Antibody. Note positive
staining of tumor cells




Cytokeratin 8                                                             Cat #: 2032-1      Size: 100ul         min. 50 slides
                                                                                           Inquire for Bulk

   Type:        Rabbit Monoclonal IgG                Clone:         EP1628Y            Species Reactivity: Human

IHC Dilution:   1:100 - 250                       Localization:     Cytoplasmic              Control:         Breast ductal carcinoma,
                                                                                                              Gastric adenocarcinoma


                                                                             Description: Keratins, the intermediate filament proteins
                                                                             of epithelial cells, connect to desmosomes, the cell-cell
                                                                             adhesion structures at the surface membrane. Among the
                                                                             various intermediate filament (IF) proteins, cytokeratin 8
                                                                             (CK-8) is especially remarkable as it is produced early in
                                                                             embryogenesis, is the only type-II CK occurring in many
                                                                             simple epithelial cells, and can also be synthesized in cer-
                                                                             tain non-epithelial cells. Keratin 8 and 18 (CK8/18) are the
                                                                             major keratin pair in simple-type epithelia, as found in the
                                                                             liver, pancreas, and intestine. CK-8 expression is specifi-
                                                                             cally seen in adenocarcinoma and ductal carcinomas of
                                                                             simple epithelia.




                                                                             Immunogen: A synthetic peptide corresponding to resi-
                                                                             dues on the C-terminus of human CK-8 was used as an
Paraffin-embedded human breast adenocarcinoma tissue stained with            immunogen.
anti-CK-8 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                          www.epitomics.com                    info@epitomics.com                       35
                                                                   info@biomol.de                  0800-2466651 www.biomol.de


                          Cytokeratin 8 Phospho (pS23)                                         Cat #: 2147-1        Size: 100ul         min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EP1629Y           Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic             Control:         Breast ductal carcinoma
Primary Antibodies




                                                                                                    Description: Keratins, the intermediate filament proteins
                                                                                                    of epithelial cells, connect to desmosomes, the cell-cell
                                                                                                    adhesion structures at the surface membrane. Among the
                                                                                                    various intermediate filament (IF) proteins, cytokeratin 8
                                                                                                    (CK-8) is especially remarkable as it is produced early in
                                                                                                    embryogenesis, is the only type-II CK occurring in many
                                                                                                    simple epithelial cells, and can also be synthesized in
                                                                                                    certain non-epithelial cells. cytokeratin 8 and 18 (CK-8/-18)
                                                                                                    are the major keratin pair in simple-type epithelia, as found
                                                                                                    in the liver, pancreas, and intestine. CK-8 expression is
                                                                                                    specifically seen in adenocarcinoma and ductal carcinomas
                                                                                                    of simple epithelia.




                                                                                                    Immunogen: A synthetic phospho-peptide corresponding
                                                                                                    to residues surrounding Ser23 of human CK-8 was used
                          Paraffin-embedded human gastric adenocarcinoma tissue stained with        as an immunogen.
                          anti-Phospho CK-8 RabMAb. Note positive staining of tumor cells




                          Cytokeratin 10                                                       Cat #: 2210-1        Size: 100ul         min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EP1607Y           Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic             Control:         Skin



                                                                                                    Description: Cytokeratins are a family of structurally related
                                                                                                    proteins that form the intermediate filament cytoskeleton
                                                                                                    in epithelial cells. Cytokeratin 10 (CK-10) is type I cyto-
                                                                                                    keratin and it is generally associated with CK-1. Defects
                                                                                                    in CK-10 have been associated with epidemolytic hyper-
                                                                                                    keratosis (EHK) and epidemolytic ichthyosis (AEI). CK-10
                                                                                                    is expressed in suprabasal layers of both kertatinizing and
                                                                                                    non-kertatinzing stratified epithelia, also in breast dutcal
                                                                                                    carcinoma and ano-rectal squamous cell carcinoma.




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues on the C-terminus of human CK-10 was used as an
                          Paraffin-embedded human skin tissue stained with anti-CK-10 RabMAb.       immunogen.
                          Note positive staining of suprabasal layers of keratinising stratified
                          epithelia



                     36    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                0800-2466651 www.biomol.de


Cytokeratin 15                                                         Cat #: 1954-1      Size: 100ul         min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EPR1614Y         Species Reactivity: Human




                                                                                                                                                 Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:      Cytoplasmic            Control:          Skin



                                                                          Description: A number of human cytokeratins are ex-
                                                                          pressed during the development of stratified epithelia from
                                                                          one-layered polar epithelia and continue to be expressed
                                                                          in several adult epithelial tissues. Cytokeratin 15 (CK-15)
                                                                          is a type I keratin without a defined type II partner whose
                                                                          expression in epidermal diseases has not been investi-
                                                                          gated. A monoclonal antibody raised against the last 17
                                                                          amino acids of the CK-15 polypeptide show that CK-15 is
                                                                          expressed primarily in the basal keratinocytes of stratified
                                                                          tissues, including the fetal epidermis and fetal nail. Although
                                                                          CK-15 in normal hair follicles was virtually absent from hair
                                                                          bulbs, it was expressed by a subset of keratinocytes in the
                                                                          outer root sheath. In human conjunctival epithelium, strong
                                                                          expression of CK-15 was observed in basal cells. No ex-
                                                                          pression of CK-15 is seen in squamous cell carcinomas.

                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                          dues on the C-terminus of human CK-15 was used as an
Paraffin-embedded human skin tissue stained with anti-CK-15 RabMAb.       immunogen.
Note positive staining of basal cells of epidermis




Cytokeratin 16                                                         Cat #: 2142-1      Size: 100ul         min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EP1615Y          Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 250                     Localization:      Cytoplasmic            Control:          Skin,
                                                                                                            Squamous cell carcinoma


                                                                          Description: The human type I keratin 16 (CK-16) is
                                                                          constitutively expressed in a number of complex epithelial
                                                                          tissues, including skin, but is better known for its induction
                                                                          under conditions favoring enhanced proliferation or abnor-
                                                                          mal differentiation, including wound healing, psoriasis, and
                                                                          cancer (squamous cell carcinomas). CK-16 is expressed in
                                                                          suprabasal interfollicular epidermis in wound healing and
                                                                          other pathological conditions associated with hyperprolifera-
                                                                          tion, such as psoriasis and are induced when keratinocytes
                                                                          are cultured in vitro. However, CK-16 is also constitutively
                                                                          expressed in normal suprabasal mucosal and palmoplantar
                                                                          keratinocytes. Pachyonychia congenita (PC) is a group of
                                                                          inherited ectodermal dysplasias, the characteristic pheno-
                                                                          type being hypertrophic nail dystrophy.


                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                          dues near the N-terminus of human CK-16 was used as
Paraffin-embedded human breast squamous cell carcinoma tissue             an immunogen.
stained with anti-CK-16 RabMAb Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                   info@epitomics.com                         37
                                                                    info@biomol.de                0800-2466651 www.biomol.de


                          Cytokeratin 17                                                         Cat #: 1960-1        Size: 100ul         min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         EPR1624Y           Species Reactivity: Human

                          IHC Dilution:   1:50 - 100                      Localization:      Cytoplasmic              Control:          Skin,
Primary Antibodies




                                                                                                                                        Squamous cell carcinoma


                                                                                                      Description: Among the members of the cytokeratin (CK)
                                                                                                      subfamily of intermediate filament (IF) proteins, CK-17 is
                                                                                                      normally expressed in the basal cells of complex epithelia
                                                                                                      but not in stratified or simple epithelia. It has an unusual
                                                                                                      expression pattern in normal and diseased states and has
                                                                                                      potential importance in tumor diagnosis. CK-17 synthesis
                                                                                                      seems to be a marker of basal cell differentiation in com-
                                                                                                      plex epithelia and therefore indicative of a certain type
                                                                                                      of epithelial “stem cells”. It has been shown that CK-17
                                                                                                      regulates cell growth through binding to the adaptor protein
                                                                                                      14-3-3sigma. These findings reveal a new and unexpected
                                                                                                      role for the intermediate filament cytoskeleton in influencing
                                                                                                      cell growth and size by regulating protein synthesis. CK-17
                                                                                                      is expressed in the nail bed, hair follicle, sebaceous glands,
                                                                                                      and other epidermal appendages.

                                                                                                      Immunogen: A synthetic peptide corresponding to resi-
                                                                                                      dues on the C-terminus of human CK-17 was used as an
                          Paraffin-embedded human breast squamous cell carcinoma tissue               immunogen.
                          stained with anti-CK-17 RabMAb Note positive staining of tumor cells




                          Cytokeratin 18                                                         Cat #: 1433-1        Size: 100ul         min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         E431-1             Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 200                     Localization:      Cytoplasmic              Control:          Gastric adenocarcinoma,
                                                                                                                                        Breast carcinoma


                                                                                                      Description: Cytokeratins play a critical role in differentia-
                                                                                                      tion and tissue specialization and function to maintain the
                                                                                                      overall structural integrity of epithelial cells. Cytokeratin
                                                                                                      polypeptides 8 and 18 (CK-8/-18) are intermediate filament
                                                                                                      phosphoglycoproteins that are expressed preferentially in
                                                                                                      glandular epithelia. CK-18 belongs to the type A (acidic)
                                                                                                      subfamily of low molecular weight keratins. CK-18 is found
                                                                                                      in most simple ductal and glandular epithelia. CK-18 is
                                                                                                      not exprresed in straitified squamous epitheium and most
                                                                                                      squamous cell carcinomas.




                                                                                                      Immunogen: A synthetic peptide corresponding to resi-
                                                                                                      dues of human CK-18 was used as immunogen.
                          Paraffin-embedded human gastric adenocarcinoma tissue stained with
                          anti-CK-18 RabMAb. Note positive staining of tumor cells




                     38    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                        info@biomol.de                0800-2466651 www.biomol.de


Cytokeratin 19                                                       Cat #: 1968-1      Size: 100ul         min. 50 slides
                                                                                      Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:        EP1580Y            Species Reactivity: Human




                                                                                                                                              Primary Antibodies
IHC Dilution:   1:250 - 500                   Localization:    Cytoplasmic              Control:         Gastric adenocarcinoma



                                                                        Description: Cytokeratin polypeptide 19 (CK-19) is a type I
                                                                        intermediate filament protein that is expressed in stratified
                                                                        and simple-type epithelia. Cytokeratins 8 and 19 concen-
                                                                        trate at costameres of striated muscle and copurify with
                                                                        the dystrophin-glycoprotein complex, perhaps through the
                                                                        interaction of the cytokeratins with the actin-binding domain
                                                                        of dystrophin. CK-19 is expressed in many ductal and
                                                                        glandular epithelia. However, CK-19 is not expressed in he-
                                                                        patocytes and considered ideal marker for liver metastasis.




                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                        dues near the C-terminus of human CK-19 was used as
Paraffin-embedded human gastric adenocarcinoma tissue stained with      an immunogen.
anti-CK-19 RabMAb. Note positive staining of tumor cells




Cytokeratin 20                                                       Cat #: 2039-1      Size: 100ul         min. 50 slides
                                                                                      Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:        EPR1622Y           Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 250                   Localization:    Cytoplasmic              Control:         Colon adenocarcinoma



                                                                        Description: Intermediate-sized filament (IF) protein des-
                                                                        ignated cytokeratin 20 (CK-20) is a major cellular protein
                                                                        of mature enterocytes and goblet cells commonly found in
                                                                        mucosal epithelium of the mammalian gastrointestinal tract.
                                                                        Results strongly suggest that transcriptional regulation of
                                                                        keratin genes in the intestinal epithelium occurs at the level
                                                                        of both immature and terminally differentiated epithelial
                                                                        cells, and is tightly regulated during both fetal development
                                                                        and crypt-to-villus differentiation of the intestinal epithe-
                                                                        lium. CK-20 is expression is commonly seen in colorectal
                                                                        carcinomas and ducal cell adenocarcinomas in pancreas,
                                                                        adenocarcinoma of the gall bladder, and Merkel cell tumors.
                                                                        CK-20 expression was significantly more prevalent in
                                                                        adenocarcinoma that originated in the gastrointestinal tract
                                                                        than that of pulmonary or breast origin.

                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                        dues on the C-terminus of human CK-20 was used as an
Paraffin-embedded human colonic adenocarcinoma tissue stained with      immunogen.
anti-CK-20 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                     www.epitomics.com                    info@epitomics.com                        39
                                                                   info@biomol.de                 0800-2466651 www.biomol.de


                          DARPP-32                                                             Cat #: 1711-1       Size: 100ul        min. 50 slides
                                                                                                                 Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:         EP721Y             Species Reactivity: Human, Mouse

                          IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic             Control:         Gastric adenocarcinoma,
Primary Antibodies




                                                                                                                                    Brain


                                                                                                   Description: The 32kDa dopamine and cyclic adenos-
                                                                                                   ine 3’,5’-monophosphate-regulated phosphoprotein
                                                                                                   (DARPP-32) can either function as a serine/threonine
                                                                                                   kinase or as a serine/threonine phosphatase depending
                                                                                                   upon which particular amino acid residue is phosphory-
                                                                                                   lated. When phosphorylated by protein kinase A (PKA)
                                                                                                   at threonine 34, DARPP-32 becomes a potent inhibitor
                                                                                                   of protein phosphatase-1. Alternatively, when phosphory-
                                                                                                   lated at threonine 75 by cyclin-dependent kinase 5(Cdk5),
                                                                                                   DARPP-32 is converted into an inhibitor of PKA. DARPP-32
                                                                                                   and truncated DARPP (t-DARPP) are overexpressed in
                                                                                                   gastric cancers.




                                                                                                   Immunogen: A synthetic peptide corresponding to resi-
                                                                                                   dues in the C-term of human DARPP-32 was used as
                          Paraffin-embedded human gastric adenocarcinoma tissue stained with       immunogen.
                          anti-DARPP-32 RabMAb. Note positive staining of tumor cells




                          Desmin                                                               Cat #: 1466-1       Size: 100ul        min. 50 slides
                                                                                                                 Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:         Y66                Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:250                          Localization:     Cytoplasmic             Control:         Muscles



                                                                                                   Description: Desmin are class-III intermediate filaments
                                                                                                   found in muscle cells, and are expressed during the forma-
                                                                                                   tion of muscle cells or fibers. Cytoskeletal intermediate
                                                                                                   filaments (IFs) are constructed from two-chain alpha-helical
                                                                                                   coiled-coil molecules arranged on an imperfect helical lat-
                                                                                                   tice. In adult striated muscle they form a fibrous network
                                                                                                   connecting myofibrils to each other and to the plasma
                                                                                                   membrane from the periphery of the Z-line structures.
                                                                                                   Desmin mutations have been linked to familial cardiac and
                                                                                                   skeletal myopathy. Desmin expression has diagnostic value
                                                                                                   in tumors of myogenic origin.




                                                                                                   Immunogen: A synthetic peptide corresponding to C-
                                                                                                   terminus of human Desmin was used as immunogen.
                          Paraffin-embedded human skeletal muscle tissue stained with anti-Des-    Predicted to cross-react with rat, bovine and pig, based
                          min RabMAb. Note positive staining of muscle fiber                       on sequence homology.




                     40    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


E-Cadherin                                                              Cat #: 1702-1      Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP700Y             Species Reactivity: Human, Mouse




                                                                                                                                                 Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:     Cell Membrane            Control:          Breast carcinoma



                                                                           Description: E-Cadherin, a member of the cadherin su-
                                                                           perfamily, is implicated as a key player in different cellular
                                                                           processes including development, morphology, polarity,
                                                                           migration and tissue integrity. E-cadherin is a glycopro-
                                                                           tein with an extracellular domain that interacts with other
                                                                           E-cadherin molecules on adjacent cells, thereby establish-
                                                                           ing adhesion between epithelial cells. Established as a
                                                                           tumor and metastasis suppressor, E-cadherin expression
                                                                           is frequently down-regulated or extinguished in malignancy
                                                                           which strongly correlates with poor prognosis. Mutations in
                                                                           E-cadherin are found in gastric, breast, colorectal, thyroid
                                                                           and ovarian cancer.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues in the 5th cadherin domain of human E-cadherin
Paraffin-embedded human breast ductal infiltrating carcinoma tissue        was used.
stained with anti-E-Cadherin RabMAb. Note positive staining of tumor
cells




EGFR                                                                    Cat #: 1902-1      Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP38Y              Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 250                     Localization:     Cell Membrane            Control:          Lymph node metastasis.
                                                                                                             Placenta or squamous
                                                                                                             cell carcinoma

                                                                           Description: Epidermal growth factor receptor (EGFR) is
                                                                           a 170 kDa transmembrane glycoprotein receptor tyrosine
                                                                           kinase that, activated by epidermal growth factor (EGF),
                                                                           affects cell growth and differentiation. Binding of EGF or
                                                                           TGF alpha to EGFR activates tyrosine kinase activity of the
                                                                           receptor. The carboxy terminal tyrosine residues on EGFR,
                                                                           Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of
                                                                           autophosphorylation, which occurs as a result of EGF bind-
                                                                           ing. Once activated, EGFR phosphotyrosines Y-1068 and
                                                                           Y-1173 mediate the binding of Grb2 to the EGFR. Com-
                                                                           monly presented in squamous cell carcinomas, EGFR is
                                                                           overexpressed in various cancers (e.g. brain, breast, lung,
                                                                           head, neck, ovary).




                                                                           Immunogen: A synthetic phospho-peptide corresponding
                                                                           to residues surrounding Tyr1068 of human EGF receptor
Paraffin-embedded human urinary bladder transitional cell carcinoma        was used as immunogen.
tissue stained with anti-EGFR RabMAb. Note positive staining of tumor
cells



 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                    info@epitomics.com                        41
                                                                     info@biomol.de                 0800-2466651 www.biomol.de


                          EGFR Phospho (pY1068)                                                    Cat #: 1727-1      Size: 100ul          min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         EP774Y             Species Reactivity: Human, Mouse

                          IHC Dilution:   1:100                            Localization:      Cell Membrane            Control:         Squamous cell carcinoma
Primary Antibodies




                                                                                                      Description: Epidermal growth factor receptor (EGFR) is
                                                                                                      a 170 kDa transmembrane glycoprotein receptor tyrosine
                                                                                                      kinase that, when activated by epidermal growth factor
                                                                                                      (EGF), affects cell growth and differentiation in normal and
                                                                                                      cancer cells. Binding of EGF or TGF alpha to EGFR acti-
                                                                                                      vates tyrosine kinase activity of the receptor. The carboxy
                                                                                                      terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148,
                                                                                                      and Tyr 1173, are the major sites of autophosphorylation,
                                                                                                      which occurs as a result of EGF binding. EGFR overexpres-
                                                                                                      sion is exhibited various cancer, such as glioma, colorectal
                                                                                                      carcinoma, breast carcinoma, and head and neck carcino-
                                                                                                      ma. The phosphorylation level of EGFR is considered one
                                                                                                      of the important predicators for outcome in non-small cell
                                                                                                      lung cancer therapy.


                                                                                                      Immunogen: A synthetic phospho-peptide corresponding
                                                                                                      to residues surrounding Tyr1068 of human EGF receptor
                          Paraffin-embedded human cervical squamous cell carcinoma tissue             was used as immunogen.
                          stained with anti-Phospho-EGFR RabMAb. Note positive staining of
                          tumor cells




                          elF-4E                                                                   Cat #: 1627-1      Size: 100ul          min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         Y449               Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                      Localization:      Predominately            Control:         Breast carcinoma
                                                                                              Cytoplasmic,
                                                                                              minor Nuclear

                                                                                                      Description: eIF-4E is a eukaryotic translation initiation
                                                                                                      factor involved in directing ribosomes to the cap structure of
                                                                                                      mRNAs. It exists in two forms: as a free form (25 kDa) and
                                                                                                      as part of a multiprotein complex eIF-4F. eIF-4E appears to
                                                                                                      be the least abundant of the initiation factors and acts as a
                                                                                                      rate-limiting step of initiation. Since translation is regulated
                                                                                                      by phosphorylation, eIF-4E phosphorylation at Ser 209
                                                                                                      by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2
                                                                                                      (Mnk2) may directly regulate the rate of protein synthesis
                                                                                                      initiation. There is also evidence that eIF-4E can function as
                                                                                                      an oncogene.




                                                                                                      Immunogen: A synthetic peptide corresponding to
                                                                                                      residues in the C-term of human eIF-4E, was used as im-
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue         munogen. The antibody detects a band on western blot of
                          stained with anti-eIF-4E RabMAb. Note positive staining of tumor cells      approximately 28 kDa.




                     42    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


Enolase2 gamma                                                       Cat #: 2588-1       Size: 100ul         min. 50 slides
                                                                                        Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EPR3377           Species Reactivity: Human




                                                                                                                                              Primary Antibodies
IHC Dilution:   1:250 - 500                     Localization:     Cell Membrane,          Control:         Brain
                                                                  Cytoplasmic


                                                                          Description: Enolase is an essential glycolytic enzyme that
                                                                          catalyses the interconversion of 2-phosphoglycerate and
                                                                          phosphoenolpyruvate. In vertebrates, there are 3 different,
                                                                          tissue-specific isoenzymes, designated alpha, beta and
                                                                          gamma. Alpha is present in most tissues, beta is localised
                                                                          in muscle tissue, and gamma is found only in nervous
                                                                          tissue. Neuron-specific enolase is released in a variety of
                                                                          neurological diseases, such as multiple sclerosis and after
                                                                          seizures or acute stroke. Several tumour cells have also
                                                                          been found positive for neuron-specific enolase. Beta-
                                                                          enolase deficiency is associated with glycogenosis type XIII
                                                                          defect.




                                                                          Immunogen: A synthetic peptide corresponding to
                                                                          residues in human Enolase2 gamma was used as an im-
Immunohistochemical analysis of paraffin-embedded human brain tissue      munogen.
using anti-NSE RabMAb. Note positive neuronal staining




Epithelial Antigen (Ep-CAM)                                          Cat #: 1144-1       Size: 100ul         min. 50 slides
                                                                                        Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         E144              Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 200                     Localization:     Cell Membrane           Control:         Breast carcinoma



                                                                          Description: Epithelial cell adhesion molecule (Ep-CAM)
                                                                          is a monomeric membrane glycoprotein that is expressed
                                                                          on virtually all epithelial cell membranes and on a variety
                                                                          of epithelial carcinomas. Ep-CAM contains an extracel-
                                                                          lular domain with two epidermal growth factor-like repeats,
                                                                          followed by a cysteine-poor region, which are necessary for
                                                                          the adhesion properties of the molecule. Ep-CAM is overex-
                                                                          pressed in a variety of human carcinomas and is, therefore,
                                                                          a potential therapeutic target for human solid tumors.




                                                                          Immunogen: A synthetic peptide corresponding to
                                                                          residues in C-terminus (cytoplasmic domain) of human
Paraffin-embedded human breast ductal infiltrating carcinoma tissue       Ep-CAM was used as immunogen.
stained with anti-Ep-CAM RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                  info@epitomics.com                       43
                                                                     info@biomol.de                  0800-2466651 www.biomol.de


                          ER alpha                                                                Cat #: 2614-1         Size: 100ul        min. 50 slides
                                                                                                                      Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         EPR703              Species Reactivity: Human

                          IHC Dilution:   1:250 - 500                       Localization:     Nuclear                   Control:         Breast Carcinoma
Primary Antibodies




                                                                                                        Description: Estrogen receptor alpha (ER alpha) is a nu-
                                                                                                        clear protein and member of the steroid hormone receptor
                                                                                                        family. ER alpha possesses both DNA binding and ligand
                                                                                                        binding domains, and exerts a significant role in activat-
                                                                                                        ing the transcription of certain genes. Ligand-dependent
                                                                                                        dimerization and phosphorylation both function to regulate
                                                                                                        the transcriptional activation of ER alpha. Particularly,
                                                                                                        phosphorylation of serines 104 and 106, located in the N-
                                                                                                        terminal transcription activation function-1 domain (AF-1),
                                                                                                        plays a large role in regulating ER alpha activity. ER alpha
                                                                                                        is highly expressed in breast carcinomas and is used to
                                                                                                        distinguishing breast carcinomas from adenocarcinomas of
                                                                                                        other primary sites.




                                                                                                      Immunogen: A synthetic peptide corresponding to resi-
                                                                                                      dues in human ER alpha was used as an immunogen.
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue
                          stained with anti-ER alpha RabMAb. Note positive staining of tumor cells




                          Ezrin                                                                   Cat #: 1729-1         Size: 100ul        min. 50 slides
                                                                                                                      Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         EP886Y              Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                       Localization:     Cell Membrane             Control:         Breast carcinoma



                                                                                                        Description: Erzin, a member of the Ezrin, Radixin, Moesin
                                                                                                        (ERM) family, is a linker protein located between cell sur-
                                                                                                        face receptors, adhesion molecules, and actin cytoskeleton.
                                                                                                        Ezrin activity is regulated by intramolecular interactions be-
                                                                                                        tween N- and C-terminal ERM association domains. Phos-
                                                                                                        phorylation at threonine 567 is a critical regulator of ezrin
                                                                                                        function allowing the active protein to link target molecules
                                                                                                        to the actin cytoskeleton. Erzin tyrosine phosphorylation can
                                                                                                        also be induced by EGF, PDGF and HGF stimulation. ERM
                                                                                                        family is expressed in epithelial tumors and may play an
                                                                                                        important role in cancer progression.




                                                                                                      Immunogen: A synthetic peptide corresponding to resi-
                                                                                                      dues near the C-terminus of human Ezrin was used as
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue         immunogen.
                          stained with anti-Ezrin RabMAb. Note positive staining of tumor cells




                     44    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                  0800-2466651 www.biomol.de


Factor XIII A                                                          Cat #: 2559-1       Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EP3372            Species Reactivity: Human




                                                                                                                                                  Primary Antibodies
IHC Dilution:   1:100 - 250                      Localization:     Cytoplasmic,            Control:          Skin
                                                                   Nuclear


                                                                           Description: Factor XIII (plasma transglutaminase, fibrin
                                                                           stabilizing factor) is a plasma protein that plays an important
                                                                           role in the final stages of blood coagulation and fibrinolysis.
                                                                           In two diseases that share some histological resemblance
                                                                           (Lymphocyte-poor graft-versus-host-reaction and toxic
                                                                           epidermal necrolysis), Factor-XIII A-positive dendrocytes
                                                                           show some morphological changes, probably as a response
                                                                           to altered cytokine environment. Factor-XIII A-positive
                                                                           dendrocytes thus are reported to possibly play a role in the
                                                                           regulation of the connective tissue remodeling that may ac-
                                                                           company epidermal destruction.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues in human Factor XIII A was used as an immunogen.
Paraffin-embedded human skin tissue stained with anti-Factor XIII
A RabMAb. Note positive staining of dendrocytes within the papillary
dermis




GCDFP15                                                                Cat #: 2186-1       Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EP1592Y           Species Reactivity: Human

IHC Dilution:   1:100 - 250                      Localization:     Cytoplasmic             Control:          Breast carcinoma



                                                                           Description: The androgen and prolactin responsive
                                                                           prolactin-inducible protein (PIP)/gross cystic disease fluid
                                                                           protein (GCDFP15) is expressed in benign and malignant
                                                                           human breast tumors and in such normal exocrine organs
                                                                           as sweat, salivary and lacrimal glands. The hormonally re-
                                                                           sponsive prolactin-inducible protein (PIP) gene is expressed
                                                                           in benign and malignant breast tumor tissues and in such
                                                                           normal exocrine organs as sweat, salivary, and lacrimal
                                                                           glands. Co-expression of GCDF15 and PSA detected in
                                                                           androgen receptor-positive breast tumors.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues near the N-term of human GCDFP15 was used as
Paraffin-embedded human breast ductal infitrating carcinoma using anti-    an immunogen.
GCDFP-15 RabMAb. Note positive staining on tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                         www.epitomics.com                   info@epitomics.com                         45
                                                                   info@biomol.de                  0800-2466651 www.biomol.de


                          GFAP                                                                Cat #: 1665-1         Size: 100ul         min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:         EP672Y             Species Reactivity: Human, Rat

                          IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic              Control:          Astrocytoma
Primary Antibodies




                                                                                                    Description: Glial fibrillary acidic protein (GFAP) belongs to
                                                                                                    the class III of the intermediate filament (IF) proteins highly
                                                                                                    specific to astrocytes which function as nerve cells in the
                                                                                                    brain and in the central nervous system (CNS) . Two other
                                                                                                    class III IF proteins, nestin and vimentin, dominate in im-
                                                                                                    mature astrocytes, while GFAP is most abundant in mature
                                                                                                    astrocytes. GFAP is used as a marker to distinguish astro-
                                                                                                    cytes form other glial cells during development. Mutations in
                                                                                                    GFAP cause Alexander disease, a rare disorder of the CNS.
                                                                                                    Most frequent mutations are located at Arginine 79 or 239 .
                                                                                                    GFAP is an important diagnostic makre for various subtypes
                                                                                                    of geiomas.




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues in the C-term of human GFAP was used as immu-
                          Paraffin-embedded human asrtocytoma (grade1) tissue stained with anti-    nogen.
                          GFAP RabMAb. Note positive staining of neoplastic astrocytes




                          HER2                                                                Cat #: 2064-1         Size: 100ul         min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:         EP1045Y            Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                    Localization:     Cell Membrane            Control:          Breast carcinoma



                                                                                                    Description: HER2 / ErbB2 is one of the four members of
                                                                                                    the ErbB receptor family of transmembrane receptor-like
                                                                                                    tyrosine kinases. The kinase activity of ErbB2 can be acti-
                                                                                                    vated without ligand if it is overexpressed, and by associa-
                                                                                                    tion with other ErbB proteins. Overexpression of ErbB2 is
                                                                                                    detected in almost 40% of human breast cancers. Bind-
                                                                                                    ing of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to
                                                                                                    poly-ubiquitination of ErbB2 and enhances its degradation.
                                                                                                    ErbB2 is one of the major targets for the treatment of breast
                                                                                                    cancer and other carcinomas.




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues near the C-terminus of human Her2 was used as an
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue       immunogen. This antibody detects Her2 phosphorylated
                          stained with anti-HER2 RabMAb. Note positive (3+) membrane staining       at Tyr1248 as well as unphosphorlyated Her2.
                          of tumor cells



                     46    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                         info@biomol.de                0800-2466651 www.biomol.de


HER2 Phospho (pY877)                                                  Cat #: 2521-1       Size: 100ul         min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:        EPR22324            Species Reactivity: Human




                                                                                                                                                Primary Antibodies
IHC Dilution:   1:250 - 500                    Localization:     Cell Membrane             Control:         Breast carcinoma



                                                                           Description: HER2 / ErbB2 is one of the four members of
                                                                           the ErbB receptor family of transmembrane receptor-like
                                                                           tyrosine kinases. The kinase activity of ErbB2 can be acti-
                                                                           vated without ligand if it is overexpressed, and by associa-
                                                                           tion with other ErbB proteins. Overexpression of ErbB2 is
                                                                           detected in almost 40% of human breast cancers. Bind-
                                                                           ing of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to
                                                                           poly-ubiquitination of ErbB2 and enhances its degradation.
                                                                           ErbB2 is one of the major targets for the treatment of breast
                                                                           cancer and other carcinomas.




                                                                         Immunogen: A phospho-specific peptide corresponding
                                                                         to residues surrounding tyrosine 877 of human HER2 was
Paraffin-embedded human breast ductal infiltrating carcinoma tissue      used as an immungen.
stained with Phospho-specific anti-HER2 RabMAb. Note positive (3+)
membrane staining of tumor cells




HIF-1 alpha                                                           Cat #: 2015-1       Size: 100ul         min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:        EP1215Y             Species Reactivity: Human, Rat

IHC Dilution:   1:100 - 250                    Localization:     Nuclear                   Control:         Hepatocellular carcinoma



                                                                           Description: The HIF1A gene encodes the HIF-1alpha
                                                                           subunit of hypoxia-inducible factor 1, a transcription fac-
                                                                           tor that is essential for cardiovascular development and
                                                                           systemic O2 homeostasis. HIF-1 is found in mammalian
                                                                           cells cultured under reduced O2 tension and is necessary
                                                                           for transcriptional activation mediated by the erythropoietin
                                                                           gene enhancer in hypoxic cells. Low oxygen level in solid
                                                                           tumor is associated with resistance to therapy. Metabolic
                                                                           targeting of HIF-1 solid tumors has been linked to enhanced
                                                                           cytotoxic chemotherapy.




                                                                         Immunogen: A synthetic peptide corresponding to resi-
                                                                         dues near the C-terminus of human HIF-1 alpha was used
Paraffin-embedded human hepatocellular carcinoma stained with anti-      as an immunogen.
HIF-1 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                       www.epitomics.com                     info@epitomics.com                       47
                                                                    info@biomol.de                 0800-2466651 www.biomol.de


                          HSP27                                                                   Cat #: 2174-1       Size: 100ul          min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:        EP1724Y              Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic                Control:         Breast carcinoma
Primary Antibodies




                                                                                            and/or Nuclear


                                                                                                      Description: Heat shock protein 27 (HSP27) is one of the
                                                                                                      small HSPs, and is involved in stress resistance and actin
                                                                                                      organization. HSP27 is expressed in response to environ-
                                                                                                      mental stresses such as heat shock, or estrogen stimulation
                                                                                                      in MCF-7 cells. High expression of phospholyated HSP27
                                                                                                      is seen in neurodegenerative diseases and various can-
                                                                                                      cers. HSP27 is highly expressed in breast cancer patients
                                                                                                      and consider important breast cancer diagnostic marker.
                                                                                                      HSP27 has been implicated in drug resistance in cancer
                                                                                                      therapy.




                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near the N-terminus of human HSP27 was used as
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue        an immunogen.
                          stained with anti-HSP27 RabMAb. Note positive staining of tumor cells




                          Ki-67                                                                   Cat #: 2642-1       Size: 100ul          min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:        EPR3611              Species Reactivity: Human

                          IHC Dilution:   1:500 - 1000                    Localization:     Nuclear                    Control:         Tonsil



                                                                                                      Description: Ki-67 antigen is a nuclear antigen specifically
                                                                                                      associated with cell proliferation. Ki-67 is expressed in all
                                                                                                      proliferating cells which are in the active phases of the cell
                                                                                                      cycle (late G1, S, G2, and mitosis), but absent in resting
                                                                                                      cells. In G1, it is predominately localized in the perinucleolar
                                                                                                      region and also found in nuclear matrix in the later phases.
                                                                                                      Ki-67 is thought to be involved in the maintaining of cell
                                                                                                      proliferation, however, its exact mechanism for function is
                                                                                                      unknown. Due to the fact that Ki-67 can be used to deter-
                                                                                                      mine the growth fraction (the proportion of the cells commit-
                                                                                                      ted to the cell cycle), it is consider an important marker in
                                                                                                      human tumor diagnostics and prognostics.




                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues in human Ki-67 was used as an immunogen.
                          Paraffin-embedded human tonsil tissue stained with anti-Ki-67 RabMAb.
                          Note nuclear staining of proliferating cells




                     48    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                        info@biomol.de                 0800-2466651 www.biomol.de


Ku80                                                                Cat #: 2620-1           Size: 100ul          min. 50 slides
                                                                                           Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:           EPR3468              Species Reactivity: Human




                                                                                                                                                   Primary Antibodies
IHC Dilution:   1:250 - 500                   Localization:       Nuclear                   Control:          Tonsil



                                                                            Description: The Ku antigen is a DNA-associated nuclear
                                                                            protein originally recognized by the sera of patients with
                                                                            autoimmune diseases. It plays a key role in multiple nuclear
                                                                            processes, such as DNA repair, chromosome maintenance,
                                                                            transcription regulation, and V(D)J recombination. The Ku
                                                                            protein is composed of 70 and 82 kDa subunits (Ku70 and
                                                                            Ku80, respectively) and contributes to genomic integrity
                                                                            through its ability to bind DNA double-strand breaks and fa-
                                                                            cilitate repair by the non-homologous end-joining pathway.
                                                                            When bound to DNA, the Ku 70/80 heterodimer enhances
                                                                            the kinase activity of the catalytic subunit of the DNA-de-
                                                                            pendent protein kinase, DNA-PKcs.




                                                                         Immunogen: A synthetic peptide corresponding to resi-
                                                                         dues in human Ku80 was used as an immunogen.
Paraffin-embedded human tonsil tissue stained with anti-Ku80 RabMAb.
Note positive staining of lymphocytes




LAT                                                                 Cat #: 1499-1           Size: 100ul          min. 50 slides
                                                                                           Inquire for Bulk

   Type:        Rabbit Monoclonal IgG            Clone:           Y109                 Species Reactivity: Human

IHC Dilution:   1:100                         Localization:       Cell Membrane             Control:          Lymph node



                                                                            Description: LAT (linker for activation of T-cells) is involved
                                                                            in the T-cell antigen receptor (TCR) signal transduction
                                                                            pathway, and may play an important role downstream in
                                                                            activating protein tyrosine kinases. LAT is phosphorylated
                                                                            by ZAP-70/Syk protein tyrosine kinases leading to recruit-
                                                                            ment of multiple signaling molecules. It has been found that
                                                                            Tyr191 and Tyr171 are required for T-cell activation and
                                                                            Tyr132 is required for the activation of PLCgamma1 and
                                                                            Ras signaling pathways, respectively. Tyr226 and Tyr191
                                                                            are required for Vav binding, whereas Tyr171 and Tyr132
                                                                            are necessary for association and activation of phos-
                                                                            phoinositide 3-kinase activity and PLCgamma1.




                                                                         Immunogen: A synthetic peptide corresponding to resi-
                                                                         dues surrounding GRB2-binding motif 2 of human LAT
Paraffin-embedded human lymph node tissue stained with anti-LAT          was used as immunogen. This antibody detects both long
RabMAb. Note positive staining of T lymphocytes                          and short forms of LAT.




 Toll Free : 1-877-7RABMAB (772-2622)                      www.epitomics.com                       info@epitomics.com                         49
                                                                   info@biomol.de                0800-2466651 www.biomol.de


                          MART-1                                                                Cat #: 1989-1        Size: 100ul        min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:        EP1422Y             Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic               Control:         Melanomas
Primary Antibodies




                                                                                                     Description: MART-1 has been widely studied as a melano-
                                                                                                     ma-specific antigen and as a melanosome-specific marker.
                                                                                                     A role for MART-1 in pigmentation is expected because
                                                                                                     its expression pattern and subcellular distribution is quite
                                                                                                     similar to the other melanosomal proteins and usually cor-
                                                                                                     relates with melanin content. MART-1 forms a complex with
                                                                                                     Pmel17 and affects its expression, stability, trafficking, and
                                                                                                     the processing which is required for melanosome structure
                                                                                                     and maturation. MART-1 is indispensable for Pmel17 func-
                                                                                                     tion and thus plays an important role in regulating mamma-
                                                                                                     lian pigmentation. A gene has been identified that encodes
                                                                                                     a melanocyte lineage-specific protein (MART-1; melanoma
                                                                                                     antigen recognized by T cells that is a widely shared mela-
                                                                                                     noma antigen recognized by the T lymphocytes of patients
                                                                                                     with established malignancy. Identification of this gene

                                                                                                   Immunogen: A synthetic peptide corresponding to resi-
                                                                                                   dues on the N-terminus of human MART-1 was used as
                          Paraffin-embedded human melanoma tissue stained with anti-MART-1         an immungen.
                          RabMAb. Note positive staining of tumor cells




                          MCM5                                                                  Cat #: 2374-1        Size: 100ul        min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:        EP2683Y             Species Reactivity: Human, Mouse

                          IHC Dilution:   1:250 - 500                    Localization:     Nuclear                   Control:         Cervical Carcinoma



                                                                                                     Description: Human nuclear proteins MCM3 and MCM5
                                                                                                     have high sequence similarities with the corresponding
                                                                                                     yeast proteins known to be required for the initiation of ge-
                                                                                                     nome replication. It has frequently been proposed that the
                                                                                                     MCM proteins may function as licensing factors for genome
                                                                                                     replication. MCM proteins are present throughout the cell
                                                                                                     cycle but are lost rapidly following differentiation. MCM
                                                                                                     proteins have linked to the assesement of tumor prognosis.




                                                                                                   Immunogen: A synthetic peptide corresponding to resi-
                                                                                                   dues near the C-terminus of human MCM5 was used as
                          Paraffin-embedded human cervical squamous cell carcinoma stained         an immunogen.
                          with anti-MCM5 (C-terminal) RabMAb. Note positive staining of tumor
                          cells



                     50    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


MCM5                                                                    Cat #: 2380-1       Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP2682Y             Species Reactivity: Human




                                                                                                                                                  Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:     Nuclear                   Control:         Cervical Carcinoma



                                                                            Description: Human nuclear proteins MCM3 and MCM5
                                                                            have high sequence similarities with the corresponding
                                                                            yeast proteins known to be required for the initiation of ge-
                                                                            nome replication. It has frequently been proposed that the
                                                                            MCM proteins may function as licensing factors for genome
                                                                            replication. MCM proteins are present throughout the cell
                                                                            cycle but are lost rapidly following differentiation. MCM
                                                                            proteins have linked to the assesement of tumor prognosis.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues near the N-term of human MCM5 was used as an
Paraffin-embedded human cervical squamous cell carcinoma tissue            immunogen.
stained with anti-MCM5 (N-terminal) RabMAb. Note positive staining of
tumor cells




Met                                                                     Cat #: 1996-1       Size: 100ul         min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP1454Y             Species Reactivity: Human

IHC Dilution:   1:100 - 250                     Localization:     Cell Membrane             Control:         Hepatocelluar carcinoma



                                                                            Description: Met is a receptor protein-tyrosine kinase
                                                                            (RPTK) for hepatocyte growth factor (HGF), which is a
                                                                            multifunctional cytokine controlling cell growth, morphogen-
                                                                            esis, and motility. Met overexpression has been identified in
                                                                            a variety of human cancers. Met kinase domain possesses
                                                                            unique features that distinguish met from other members
                                                                            of the src family of protein tyrosine kinases. Hereditary
                                                                            papillary renal carcinoma (HPRC) is a recently recognized
                                                                            form of inherited kidney cancer Results suggest that mis-
                                                                            sense mutations located in the MET proto-oncogene lead to
                                                                            constitutive activation of the Met protein and papillary renal
                                                                            carcinomas. Heptaoma cells express functional c-MET,
                                                                            which may provide a target for theraputic treatment of hu-
                                                                            man metastases.


                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues near the N-terminus of human Met was used as an
Paraffin-embedded human hepatocellular carcinoma tissue stained with       immunogen.
anti-Met RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                     info@epitomics.com                        51
                                                                   info@biomol.de                  0800-2466651 www.biomol.de


                          MMP-1                                                                Cat #: 1973-1         Size: 100ul         min. 50 slides
                                                                                                                    Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EP1247Y            Species Reactivity: Human

                          IHC Dilution:   1:50 - 100                      Localization:     Cytoplasmic              Control:          Placenta
Primary Antibodies




                                                                                                     Description: The matrix metalloproteinases (MMPs) are
                                                                                                     a family of closely related proteolytic enzymes which are
                                                                                                     involved in the degradation of different components of the
                                                                                                     extracellular matrix. There is increasing evidence to indicate
                                                                                                     that individual MMPs have an important role in tumor inva-
                                                                                                     sion and tumour spread.Human interstitial collagenase
                                                                                                     (matrix metalloproteinase-1, MMP-1), an enzyme whose
                                                                                                     only known physiologic substrate has heretofore been
                                                                                                     believed to be the extracellular matrix molecule, collagen.
                                                                                                     Data indicate that matrix metalloproteinase-1 displays an
                                                                                                     expanded substrate repertoire that supports the existence
                                                                                                     of a new interface between connective tissue turnover and
                                                                                                     serine proteinase inhibitors. MMP-1 in the stromal-tumor
                                                                                                     microenvironment can alter the behavior of cancer cells
                                                                                                     through PAR1 to promote cell migration and invasion.

                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues on human MMP-1 was used as an immunogen.
                          Paraffin-embedded human placental villi tissue stained with anti-MMP-1
                          RabMAb. Note positive staining of trophoblasts




                          MMP-2                                                                Cat #: 1842-1         Size: 100ul         min. 50 slides
                                                                                                                    Inquire for Bulk

                             Type:        Rabbit Polyclonal IgG              Clone:         Polyclonal         Species Reactivity: Human
                                          Affinity Purified
                          IHC Dilution:   1:50 - 100                      Localization:     Cytoplasmic              Control:          Placenta


                                                                                                     Description: The matrix metalloproteinases (MMPs) are
                                                                                                     a family of closely related proteolytic enzymes which are
                                                                                                     involved in the degradation of different components of the
                                                                                                     extracellular matrix. There is increasing evidence to indicate
                                                                                                     that individual MMPs have an important role in tumor inva-
                                                                                                     sion and tumour spread. MMP-2, also known as Gelatinase
                                                                                                     A, is a type IV collagenase that specifically cleaves type
                                                                                                     IV collagen, the major structural component of basement
                                                                                                     membranes. The metastatic potential of tumor cells has
                                                                                                     been found to correlate with the activity of this enzyme.
                                                                                                     MMP-2 is regulated differently from other MMPs, suggest-
                                                                                                     ing that it may play a unique role in cell-matrix interactions,
                                                                                                     including cell invasion. The conversion from the 72 kDa
                                                                                                     progelatinase A to the active 62 kDa species may be a key
                                                                                                     event in the acquisition of invasive potential

                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near the C-terminus of human MMP-2 was used as
                          Paraffin-embedded human placental villi tissue stained with anti-MMP-2     an immunogen.
                          Rabbit Polyclonal antibody. Note positive staining of trophoblasts




                     52    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


MMP-3                                                                Cat #: 1908-1        Size: 100ul          min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP1186Y            Species Reactivity: Human, Mouse, Rat




                                                                                                                                                  Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic              Control:         Placenta



                                                                          Description: The matrix metalloproteinases (MMPs) are
                                                                          a family of closely related proteolytic enzymes which are
                                                                          involved in the degradation of different components of the
                                                                          extracellular matrix. There is increasing evidence to indicate
                                                                          that individual MMPs have an important role in tumor inva-
                                                                          sion and tumour spread. Matrix metalloproteinase-3 (MMP-
                                                                          3) is a protein involved in tumor invasion and metastasis.
                                                                          MMP-3 (stromelysin) is a secreted metalloprotease with a
                                                                          wide range of substrate specificities. Data indicate that the
                                                                          expression and the possible involvement of secreted metal-
                                                                          loproteases in tumorigenesis result from a specific interac-
                                                                          tion between the transforming factor and the target cell,
                                                                          which may vary in different species.




                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                          dues near the C-terminus of human MMP-3 was used as
Paraffin-embedded human placental villi tissue stained with anti-MMP-3    an immunogen.
RabMAb. Note positive staining of trophoblasts




MMP-8                                                                Cat #: 2145-1        Size: 100ul          min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP1252Y            Species Reactivity: Human

IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic              Control:         Placenta



                                                                          Description: The matrix metalloproteinases (MMPs) are
                                                                          a family of closely related proteolytic enzymes which are
                                                                          involved in the degradation of different components of the
                                                                          extracellular matrix. There is increasing evidence to indicate
                                                                          that individual MMPs have an important role in tumor inva-
                                                                          sion and tumour spread. Matrix Metalloporteinase 8 (MMP-
                                                                          8, Collagenase-2, Neutrophil Collagenase) functions mainly
                                                                          to degrade fibrillar type I, II, and III collagens, serpins, and
                                                                          aggrecan. MMP-8 degrades by cleaving at one specific
                                                                          on the fibrillar collagens with higher cleave activity on type
                                                                          I compared to type III collagens. MMP-8 is expressed in
                                                                          neutrophils, chondrocytes, and gingival fibroblasts.




                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                          dues in human MMP-8 was used as an immunogen.
Paraffin-embedded human placental villi tissue stained with anti-MMP-8
RabMAb. Note positive staining of trophoblasts




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                    info@epitomics.com                         53
                                                                    info@biomol.de                  0800-2466651 www.biomol.de


                          MMP-9                                                                Cat #: 1939-1        Size: 100ul         min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EP1255Y            Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic              Control:         Placenta
Primary Antibodies




                                                                                                     Description: The matrix metalloproteinases (MMPs) are
                                                                                                     a family of closely related proteolytic enzymes which are
                                                                                                     involved in the degradation of different components of the
                                                                                                     extracellular matrix. There is increasing evidence to indi-
                                                                                                     cate that individual MMPs have an important role in tumor
                                                                                                     invasion and tumour spread. 92-kDa Type IV collagenase
                                                                                                     (MMP-9) is believed to play a critical role in physiological
                                                                                                     tissue-remodeling processes and also in many pathological
                                                                                                     conditions such as tumor invasion. MMP-9 is predominantly
                                                                                                     expressed in neutrophils, macrophages, and mast cells,
                                                                                                     rather than in oncogene-positive neoplastic cells.




                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near the C-terminus of human MMP-9 was used as
                          Paraffin-embedded human placental villi tissue stained with anti-MMP-9     an immunogen.
                          RabMAb. Note positive staining of trophoblasts




                          MMP-11                                                               Cat #: 1881-1        Size: 100ul         min. 50 slides
                                                                                                                   Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG              Clone:         EP1259Y            Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic              Control:         Placenta



                                                                                                     Description: The matrix metalloproteinases are a tightly
                                                                                                     regulated family of enzymes that degrade extracellular
                                                                                                     matrix and basement membrane components. Recent
                                                                                                     evidence suggests that these proteases and their specific
                                                                                                     inhibitors play important roles in normal developmental pro-
                                                                                                     cesses and in pathological conditions. MMP-11 (stromely-
                                                                                                     sin-3), a new member of the matrix metalloproteinase fam-
                                                                                                     ily, is expressed in tissues undergoing the active remodeling
                                                                                                     associated with embryonic development, wound healing
                                                                                                     and tumor invasion. But like all other members of the matrix
                                                                                                     metalloproteinase gene family, stromelysin-3 is synthesized
                                                                                                     as an inactive precursor that must be processed to its
                                                                                                     mature form in order to express enzymic activity. MMP-11,
                                                                                                     like an inflammatory mediator, may exert a chemotactic
                                                                                                     influence on macrophages which aggregate in the mesan-

                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near the C-terminus of human MMP-11 was used as
                          Paraffin-embedded human placental villi tissue stained with anti-MMP-11    an immunogen.
                          RabMAb. Note positive staining of trophoblasts




                     54    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                         info@biomol.de                   0800-2466651 www.biomol.de


MMP-12                                                               Cat #: 1906-1         Size: 100ul         min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP1261Y            Species Reactivity: Human, Mouse, Rat




                                                                                                                                                 Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic              Control:         Placenta



                                                                           Description: Human macrophage elastase (MMP-12) is a
                                                                           member of the family of matrix metalloproteinases (MMPs)
                                                                           that plays, like other members of the family, an impor-
                                                                           tant role in inflammatory processes contributing to tissue
                                                                           remodeling and destruction. In particular, a prominent role
                                                                           of MMP-12 in the destruction of elastin in the lung alveolar
                                                                           wall and the pathogenesis of emphysema has been sug-
                                                                           gested. It is therefore an attractive therapeutic target. MMP-
                                                                           12 is expressed mainly in alveolar macrophages has been
                                                                           identified in the mouse lung as the main destructive agent
                                                                           associated with cigarette smoking, which gives rise to em-
                                                                           physema, both directly via elastin degradation and indirectly
                                                                           by disturbing the proteinase/antiproteinase balance via
                                                                           inactivation of the alpha1-proteinase inhibitor (alpha1-PI),
                                                                           the antagonist of the leukocyte elastase. Because elastin

                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues on the C-terminus of human MMP-12 was used as
Paraffin-embedded human placental villi tissue stained with anti-MMP-12    an immunogen.
RabMAb. Note positive staining of trophoblasts




MMP-21                                                               Cat #: 1955-1         Size: 100ul         min. 50 slides
                                                                                         Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP1277Y            Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic              Control:         Placenta



                                                                           Description: The matrix metalloproteinases (MMPs) are
                                                                           a family of closely related proteolytic enzymes which are
                                                                           involved in the degradation of different components of the
                                                                           extracellular matrix. There is increasing evidence to indi-
                                                                           cate that individual MMPs have an important role in tumor
                                                                           invasion and tumour spread. Expression of MMP-21 is
                                                                           controlled uniquely by Pax and Notch transcription factors
                                                                           known to be critical for organogenesis. . MMP-21 is ex-
                                                                           pressed in various human fetal and adult tissues as well as
                                                                           in cancer cell lines. MMP-21 protein can also be detected in
                                                                           malignancies such as ovarian and colon carcinomas by im-
                                                                           munohistochemical staining. Findings suggest that MMP-21
                                                                           functions in embryogenesis and tumor progression. Unlike
                                                                           many classical MMPs, it is present in various normal adult
                                                                           tissues. Among epithelial MMPs, MMP-21 has a unique

                                                                           Immunogen: A synthetic peptide that corresponds to resi-
                                                                           dues on human MMP-21 were used as an immunogen.
Paraffin-embedded human placental villi tissue stained with anti-MMP-21    This antibody recognizes 2 isoforms of MMP-21.
RabMAb. Note positive staining of trophoblasts




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                    info@epitomics.com                        55
                                                                    info@biomol.de                  0800-2466651 www.biomol.de


                          Moesin                                                                   Cat #: 2036-1      Size: 100ul          min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         EP1863Y             Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                      Localization:     Cell Membrane,            Control:         Breast carcinoma
Primary Antibodies




                                                                                             Cytoplasmic


                                                                                                      Description: Moesin (membrane-organizing extension spike
                                                                                                      protein) has previously been characterized as a possible re-
                                                                                                      ceptor protein for heparan sulfate and also as a cytoskeletal
                                                                                                      linker protein that stabilizes cell surface microvilli, filopodia
                                                                                                      and lamellipodia. Data indicate that moesin is identical to
                                                                                                      the 77-kDa band that copurifies with ezrin in its isolation
                                                                                                      from human placenta. Members of the ezrin-radixin-moesin
                                                                                                      (ERM) family of membrane-cytoskeletal linking proteins
                                                                                                      have NH2- and COOH-terminal domains that associate with
                                                                                                      the plasma membrane and the actin cytoskeleton, respec-
                                                                                                      tively. ERM family proteins are expresed in epithelia tumors
                                                                                                      and may play an important role in cancer progression.




                                                                                                      Immunogen: A synthetic peptide corresponding to resi-
                                                                                                      dues near the C-terminus of human Moesin was used as
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue         an immunogen.
                          stained with anti-Moesin RabMAb. Note positive staining of tumor cells




                          mTOR                                                                     Cat #: 1612-1      Size: 100ul          min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         Y391                Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                      Localization:     Cytoplasmic               Control:         Prostate carcinoma



                                                                                                      Description: The mammalian target of rapamycin (mTOR)
                                                                                                      is a major effector of cell growth and proliferation via the
                                                                                                      regulation of protein synthesis. mTOR regulates protein
                                                                                                      synthesis through the phosphorylation and inactivation of
                                                                                                      the repressor of mRNA translation 4E-BP1 and though the
                                                                                                      phosphorylation and activation of S6 kinase. Inhibition of
                                                                                                      mTOR signaling pathway has been shown as a potentially
                                                                                                      useful theratputic strategy treatment of advanced prostate
                                                                                                      cancer.




                                                                                                      Immunogen: A synthetic peptide corresponding to
                                                                                                      residues near the C-term of PI3K/PI4K domain of human
                          Paraffin-embedded human prostatic adenocarcinoma tissue stained with        mTOR was used as an immunogen
                          anti-mTOR RabMAb. Note positive staining of tumor cells




                     56    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                         info@biomol.de                 0800-2466651 www.biomol.de


Myoglobin                                                            Cat #: 2472-1        Size: 100ul         min. 50 slides
                                                                                        Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:           EP3081Y          Species Reactivity: Human, Mouse, Rat




                                                                                                                                                Primary Antibodies
IHC Dilution:   1:250 - 500                    Localization:       Cytoplasmic            Control:         Skeletal muscle



                                                                          Description: Myoglobin, an intracellular haemoprotein
                                                                          expressed in the heart and oxidative skeletal myofibres
                                                                          of vertebrates, binds molecular oxygen and may facilitate
                                                                          oxygen transport from erythrocytes to mitochondria, thereby
                                                                          maintaining cellular respiration during periods of high physi-
                                                                          ological demand. Myoglobin may serve a variety of func-
                                                                          tions in muscular oxygen supply, such as oxygen storage,
                                                                          facilitated O(2) diffusion, and myoglobin-mediated oxidative
                                                                          phosphorylation. Disruption of myoglobin results in the acti-
                                                                          vation of multiple compensatory mechanisms that steepen
                                                                          the pO(2) gradient and reduce the diffusion path length for
                                                                          O(2) between capillary and the mitochondria.




                                                                         Immunogen: A synthetic peptide corresponding to resi-
                                                                         dues on the C-terminus of human myglobin was used as
Paraffin-embedded human heart tissue stained with anti-Myoglobin         an immunogen.
RabMAb. Note positive staining of cardiac muscles




Nm23-H1                                                              Cat #: 2578-1        Size: 100ul         min. 50 slides
                                                                                        Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:           EPR3036          Species Reactivity: Human

IHC Dilution:   1:250 - 500                    Localization:       Cytoplasmic            Control:         Lung carcinoma



                                                                          Description: Nm23 protein is a metastasis suppressor
                                                                          protein, expressed in all tissues. Reduced Nm23 expres-
                                                                          sion is related to a high incidence of lymph node and distant
                                                                          metastasis and poor prognosis in patients with cancers.




                                                                         Immunogen: A synthetic peptide corresponding to resi-
                                                                         dues in human Nm23-H1 was used as an immunogen.
Paraffin-embedded human lung adenocarcinoma tissue stained with anti-
Nm23-H1 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                       www.epitomics.com                    info@epitomics.com                        57
                                                                   info@biomol.de                0800-2466651 www.biomol.de


                          NPM                                                               Cat #: 2041-1           Size: 100ul         min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:        EP1848Y             Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                    Localization:    Nuclear                   Control:         Tonsil
Primary Antibodies




                                                                                                    Description: Nucleophosmin (NPM), a nucleocytoplasmic
                                                                                                    shuttling protein with prominent nucleolar localization,
                                                                                                    regulates the ARF-p53 tumor-suppressor pathway. Translo-
                                                                                                    cations involving the NPM gene cause cytoplasmic disloca-
                                                                                                    tion of the NPM protein. NPM is a major nucleolar protein
                                                                                                    which is 20 times more abundant in tumor or proliferating
                                                                                                    cells than in normal resting cells. Myeloid leukemia factor 1
                                                                                                    (MLF1) binds NPM and studies have indicated that MLF1 is
                                                                                                    normally located in the cytoplasm, whereas NPM-MLF1 is
                                                                                                    targeted to the nucleus, with highest levels in the nucleolus.
                                                                                                    The nuclear/nucleolar localization of NPM-MLF1 mirrors
                                                                                                    that of NPM, indicating that NPM trafficking signals direct
                                                                                                    MLF1 to an inappropriate cellular compartment in myeloid
                                                                                                    leukemia cells.


                                                                                                  Immunogen: A synthetic peptide corresponding to resi-
                                                                                                  dues on the N-terminus of human NPM was used as an
                          Paraffin-embedded human tonsils tissue stained with anti-NPM RabMAb.    immunogen
                          Note positive staining of lymphocytes




                          OCA B                                                             Cat #: 2553-1           Size: 100ul         min. 50 slides
                                                                                                                  Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:        EP3300              Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                    Localization:    Nuclear                   Control:         Tonsil



                                                                                                    Description: OCA-B is a lymphoid-specific transcrip-
                                                                                                    tional coactivator which interacts with the transcription
                                                                                                    factors Oct-1 and Oct-2. It has been shown to be critical
                                                                                                    for development of a normal immune response, where it
                                                                                                    mediates octamer-dependent transcriptional activity in B
                                                                                                    lymphocytes. It is also critically involved in the formation
                                                                                                    of germinal centers in secondary lymphoid organs. OCA-B
                                                                                                    levels have been observed to be massively upregulated in
                                                                                                    germinal center B cells, as compared with resting B cells.




                                                                                                  Immunogen: A synthetic peptide corresponding to resi-
                                                                                                  dues in human OCA-B was used as an immunogen.
                          Paraffin-embedded human tonsil tissue stained with anti-OCA-B
                          RabMAb. Note positive staining of B lymphocytes




                     58    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                           info@biomol.de                  0800-2466651 www.biomol.de


Oct2                                                                   Cat #: 2652-1         Size: 100ul          min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EPR542               Species Reactivity: Human




                                                                                                                                                   Primary Antibodies
IHC Dilution:   1:250 - 500                      Localization:     Nuclear                   Control:          Tonsil



                                                                             Description: Octamer transcription factor-2 (Oct-2) is a
                                                                             transcription factor that specifically binds to the octamer
                                                                             motif (5- ATTTCAT-3). Mostly expressed in B cells, Oct-2
                                                                             posses a leucine zipper domain and belongs to the POU
                                                                             family of transcription factors. Additionally, Oct-2 regulates
                                                                             transcription in a number of tissues in addition to activating
                                                                             immunoglobulin gene expression.




                                                                            Immunogen: A synthetic peptide corresponding to resi-
                                                                            dues in human Oct-2 was used as an immunogen.
Paraffin-embedded human tonsil tissue stained with anti-Oct-2 RabMAb.
Note positive staining of B lymphocytes




p27/Kip1                                                               Cat #: 1591-1         Size: 100ul          min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         Y236                 Species Reactivity: Human, Rat

IHC Dilution:   1:100 - 250                      Localization:     Nuclear                   Control:          Breast carcinoma



                                                                             Description: p27/Kip1 is a cyclin-dependent kinase inhibi-
                                                                             tor involved in G1 arrest. p27/Kip1 binds to and inhibits
                                                                             cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4.
                                                                             p27/Kip1 is regulated by phosphorylation on serine 10 (S10)
                                                                             and threonine 187 (T187). Phosphorylation by CDK2 on
                                                                             T187 results in ubiquitylation and degradation of p27/Kip1;
                                                                             while phosphorylation by hKIS on S10 signals the nuclear
                                                                             export to the cytoplasm. Low expression of p27/Kip1 has
                                                                             been linked to por prognosis in various cancers (e.g. colon,
                                                                             small breast, hepatocelluarl, myeloma)




                                                                            Immunogen: A synthetic peptide corresponding to
                                                                            residues in the C-term of human p27/Kip1 was used as
Paraffin-embedded human breast ductal infiltrating carcinoma tissue         immunogen
stained with anti-p27/Kip1 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                         www.epitomics.com                     info@epitomics.com                        59
                                                                    info@biomol.de                0800-2466651 www.biomol.de


                          p53                                                                    Cat #: 1005-1        Size: 100ul         min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:        Y5                   Species Reactivity: Human, Rat

                          IHC Dilution:   1:100 - 250                     Localization:     Nuclear                   Control:          Bladder carcinoma
Primary Antibodies




                                                                                                      Description: p53 acts as both a tumor-suppressor and
                                                                                                      transcription factor that, upon activation by DNA damage
                                                                                                      and other cellular stress signals, leads to the transcription
                                                                                                      of genes triggering cell-cycle arrest, apoptosis, and DNA
                                                                                                      repair. The gene for the nuclear phosphoprotein p53 is
                                                                                                      the most commonly mutated gene yet identified in human
                                                                                                      cancers. p53 is frequently mutated or inactivated in about
                                                                                                      60% of cancers.




                                                                                                    Immunogen: A synthetic peptide corresponding to N-
                                                                                                    terminal residues human p53 was used as immunogen.
                          Paraffin-embedded human urinary bladder transitional cell carcinoma       The antibody should recognize human wild-type and
                          tissue stained with anti-p53 RabMAb. Note positive staining of tumor      mutant p53.
                          cells




                          PAK1                                                                   Cat #: 1705-1        Size: 100ul         min. 50 slides
                                                                                                                     Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:        EP656Y               Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 200                     Localization:     Cytoplasmic               Control:          Colon carcinoma



                                                                                                      Description: p21-activated kinase 1 (PAK1) is a member of
                                                                                                      a family of serine/threonine protein kinase defined by their
                                                                                                      interaction with the small GTPases, Cdc42 and Rac. The
                                                                                                      PAK proteins can be essentially divided into two categories,
                                                                                                      groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5
                                                                                                      and PAK6), based on their structures. PAK1 participates in
                                                                                                      regulating the actin cytoskeleton, focal adhesion contacts,
                                                                                                      cell motility, apoptosis and transcription. It also promotes
                                                                                                      the disassembly of stress fibers. PAK1 also binds PIX (PAK-
                                                                                                      binding protein), COOL and adapter protein Nck. PAK1
                                                                                                      is overexpressed in various human tumors (breast, colon,
                                                                                                      hepatocelluar) and its overexpression is associated to ag-
                                                                                                      gressive tumor behaviour.




                                                                                                    Immunogen: A synthetic peptide corresponding to resi-
                                                                                                    dues in the human PAK1 protein was used as immuno-
                          Paraffin-embedded human gastric adenocarcinoma tissue stained with        gen.
                          anti-PAK1 RabMAb. Note positive staining of tumor cells




                     60    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


PARP-1 (Cleaved p25)                                                 Cat #: 1051-1         Size: 100ul          min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         E51                 Species Reactivity: Human, Mouse




                                                                                                                                               Primary Antibodies
IHC Dilution:   1:100                           Localization:     Nuclear                  Control:          Breast carcinoma



                                                                            Description: Poly (ADP-ribose) polymerase (PARP) is
                                                                            zinc-dependent DNA binding protein that recognizes DNA
                                                                            strand breaks and is presumed to play a role in DNA repair.
                                                                            As a marker for apoptosis, PARP is cleaved in vitro by
                                                                            many caspases, and in vivo by Caspase-3. Existing as a
                                                                            116 kDa nuclear protein, PARP is cleaved between amino
                                                                            acids Asp214 and Gly215 to yield two fragments of 29 kDa
                                                                            (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-
                                                                            binding domain).




                                                                          Immunogen: A synthetic peptide corresponding to
                                                                          residues before the cleavage site of human PARP-1 was
Paraffin-embedded human breast ductal infiltrating carcinoma tissue       used as immunogen. The antibody only recognize p25
stained with anti-PARP-1 (Cleaved p25) RabMAb. Note positive staining     cleaved-form of PARP-1.
of tumor cells




PAX2                                                                 Cat #: 2549-1         Size: 100ul          min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP3251              Species Reactivity: Human, Mouse

IHC Dilution:   1:1000 - 2500                   Localization:     Nuclear                  Control:          Renal cell carcinoma,
                                                                                                             Fetal kidney


                                                                            Description: PAX2 is a transcription factor that plays a
                                                                            key role during renal development and angiogenesis and
                                                                            through the Akt-survival pathway. PAX2 is also expressed in
                                                                            normal renal medulla where it is regulated by the normally
                                                                            high and variable NaCl concentration, and it protects renal
                                                                            medullary cells from high NaCl concentration-induced
                                                                            apoptosis. PAX2 is expressed in primitive cells of the kid-
                                                                            ney, ureter, eye, ear and central nervous system. Recent
                                                                            reports show that PAX2 also plays a role in breast cancer
                                                                            by competing with the ER co-activator AIB-1/SRC-3 for the
                                                                            binding and regulation of ERBB2 transcription. PAX2 has
                                                                            been identified as a highly specific marker for renal cell
                                                                            carcinoma.




                                                                          Immunogen: A synthetic peptide corresponding to resi-
                                                                          dues in human PAX2 was used as an immunogen.
Paraffin-embedded human renal cell carcinoma tissue stained with anti-
PAX2 RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                    info@epitomics.com                      61
                                                                      info@biomol.de                  0800-2466651 www.biomol.de


                          Paxillin                                                                   Cat #: 1500-1      Size: 100ul          min. 50 slides
                                                                                                                       Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                 Clone:         Y113                Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                       Localization:      Cytoplasmic               Control:         Breast carcinoma
Primary Antibodies




                                                                                                        Description: Paxillin is a cytoskeletal protein involved in
                                                                                                        actin-membrane attachment at sites of cell adhesion to the
                                                                                                        extracellular matrix (focal adhesion). Paxillin plays a signifi-
                                                                                                        cant role in integrin signaling and the tyrosine phosphoryla-
                                                                                                        tion of paxillin is required for integrin-mediated cytoskeletal
                                                                                                        reorganization. Paxillin is phosphorylated by focal adhesion
                                                                                                        kinase (FAK) at Tyr118. It has been found that phosphoryla-
                                                                                                        tion of paxillin at both Tyr118 and Tyr31 is essential for MM1
                                                                                                        cancer cell migration. Paxillin is involved in cell adhesion
                                                                                                        and migration, and plays in role embryogenesis, inflamma-
                                                                                                        tion, and various cancers.




                                                                                                        Immunogen: A synthetic peptide corresponding to
                                                                                                        residues in N-terminus of human Paxillin was used as im-
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue           munogen. This antibody detects alpha, beta and gamma
                          stained with anti-Paxillin RabMAb. Note positive staining of tumor cells      isoforms of Paxillin.




                          PDCD4                                                                      Cat #: 2619-1      Size: 100ul          min. 50 slides
                                                                                                                       Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                 Clone:         EPR3431             Species Reactivity: Human

                          IHC Dilution:   1:250 - 500                       Localization:      Cytoplasmic,              Control:         Tonsil
                                                                                               Nuclear


                                                                                                        Description: PDCD4 encodes a protein localized to the
                                                                                                        nucleus of proliferating cells. PDCD4 expression is modu-
                                                                                                        lated by cytokines in natural killer and T cells. PDCD4 has
                                                                                                        been identified as a tumor supressor which inhibits neoplas-
                                                                                                        tic transformation and tumor invasion.




                                                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                                                        dues in human PDCD4 was used as an immunogen.
                          Paraffin-embedded human tonsil tissue stained with anti-PDCD4
                          RabMAb. Note positive staining of T lymphocytes




                     62    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


Pim-1                                                                   Cat #: 2409-1      Size: 100ul          min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:        EP2645Y             Species Reactivity: Human




                                                                                                                                                    Primary Antibodies
IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic,              Control:         Prostatic adenocarcinoma
                                                                  Nuclear


                                                                           Description: Protein kinase Pim-1 is a serine/threonine
                                                                           kinase that has been implicated in the development of he-
                                                                           matopoietic and prostatic malignancies. Two isoforms, the
                                                                           44 and 33 kDa Pim-1, are expressed in all human prostate
                                                                           cancer cell lines examined. The subcellular localization of
                                                                           human 44 kDa Pim-1 is primarily on the plasma membrane,
                                                                           while the 33 kDa isoform is present in both the cytosol and
                                                                           nucleus in PCA cells. Studies imply a physiological role of
                                                                           the Pim-1 protooncogene during hematopoietic develop-
                                                                           ment and a deregulation in various leukemias. expressed in
                                                                           circulating granulocytes. With its high expression, Pim-1 is
                                                                           upregulated in prostate cancer.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues in human Pim-1 was used as a immunogen.
Paraffin-embedded human prostatic adenocarcinoma tissue stained with
anti-Pim-1 RabMAb. Note positive staining of tumor cells




PLAP                                                                    Cat #: 1566-1      Size: 100ul          min. 50 slides
                                                                                          Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:        Y102                Species Reactivity: Human

IHC Dilution:   1:50 - 100                      Localization:     Cell membrane             Control:         Placenta,
                                                                                                             Seminoma


                                                                           Description: Phospholipase A2 activator protein (PLAP),
                                                                           activates PLA2 (phospholipase A2 enzyme) and is an
                                                                           important mediator of eicosanoid generation. Also known as
                                                                           a pro-inflammatory agent, PLAP has been found in inflamed
                                                                           tissues and synovial fluid from patients with rheumatoid ar-
                                                                           thritis. As such, it is believed to play an important role in the
                                                                           regulation of inflammatory diseases. The formation of PLAP
                                                                           can be stimulated by IL-1 beta and TNF-alpha. PLAP is
                                                                           used as a diagonistic marker for testicular germ cell tumor.




                                                                           Immunogen: A synthetic peptide corresponding to
                                                                           residues near the N-term of human PLAP was used as
Paraffin-embedded human placental villi tissue stained with anti-PLAP      immunogen.
RabMAb. Note positive staining of trophoblasts




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                     info@epitomics.com                          63
                                                                      info@biomol.de                   0800-2466651 www.biomol.de


                          PR                                                                      Cat #: 1483-1          Size: 100ul         min. 50 slides
                                                                                                                       Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                 Clone:         Y85                 Species Reactivity: Human, Rat

                          IHC Dilution:   1:100 - 250                       Localization:      Nuclear                   Control:         Breast carcinoma
Primary Antibodies




                                                                                                         Description: The human progesterone receptor (PR), is
                                                                                                         a ligand-activated transcription factor and is a member of
                                                                                                         the steroid receptor family. PR exists in humans as two
                                                                                                         isoforms; PR-A (94 kDa) which lacks the first 164 amino
                                                                                                         acids of PR-B and PR-B (114 kDa). While the two forms
                                                                                                         of PR have similar DNA- and ligand-binding affinities they
                                                                                                         have opposite transcriptional activities. PR-B functions as
                                                                                                         an activator of progesterone-responsive genes, while PR-A
                                                                                                         functions as a strong transdominant repressor of PR-B.
                                                                                                         Expression of PR has been linked to prediction of likely re-
                                                                                                         sponse to endocrine therapy against human breast cancer.




                                                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                                                        dues near N-terminus of human Progesterone Receptor
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue           was used as immunogen. The antibody does not cross-
                          stained with anti-PR RabMAb. Note positive staining of tumor cells            react with other NR3 family members.




                          Prohibitin                                                              Cat #: 2410-1          Size: 100ul         min. 50 slides
                                                                                                                       Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                 Clone:         EP2803Y             Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:250 - 500                       Localization:      Cytoplasmic               Control:         Heart,
                                                                                                                                          Breast carcinoma


                                                                                                         Description: Prohibitin (PHB) proteins have been ascribed
                                                                                                         various functions, including cell cycle regulation, apoptosis,
                                                                                                         assembly of mitochondrial respiratory chain enzymes, and
                                                                                                         aging. It has been shown that the mammalian prohibitins
                                                                                                         are present in the inner mitochondrial membrane and are
                                                                                                         always bound to each other, with no free protein detectable.
                                                                                                         They are coexpressed during development and in adult
                                                                                                         mammalian tissues, and expression levels are indicative
                                                                                                         of a role in mitochondrial metabolism, but are not compat-
                                                                                                         ible with roles in the regulation of cellular proliferation or
                                                                                                         apoptosis. Results suggest that prohibitin may be a tumor
                                                                                                         suppressor and is associated with tumor development and/
                                                                                                         or progression of at least some breast cancers.




                                                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                                                        dues on the N-terminus of human prohibitin was used as
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue           an immunogen.
                          stained with anti-Prohibitin RabMAb. Note positive staining of tumor cells




                     64    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                         info@biomol.de              0800-2466651 www.biomol.de


Prostate-Specific Antigen                                          Cat #: 1984-1        Size: 100ul        min. 50 slides
                                                                                      Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:         EP1588Y           Species Reactivity: Human




                                                                                                                                             Primary Antibodies
IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic            Control:         Normal prostate,
                                                                                                         Prostatic carcinoma


                                                                        Description: Prostate-specific antigen (PSA, Kallikrein-3,
                                                                        KLK3) is a serine protease that is usually secreted from the
                                                                        prostate gland and presented in seminal plasma. PSA func-
                                                                        tion in the liquefaction of seminal coagulum, through hydro-
                                                                        lysis of the high molecular mass seminal vesicle protein. Its
                                                                        substrates includes semenogelin I & II, fibronectin, insulin-
                                                                        like growth factor binding protein 3 (IGBP3), and laminin.
                                                                        Highly expressed by normal and malignant prostate cancer
                                                                        cells, PSA has been implicated in the growth, invasion,
                                                                        and metastasis of prostate cancer cells. Therefore, PSA
                                                                        is widely used as a diagnostic marker for prostate cancer.
                                                                        Recently, PSA has been suggested as an endothelial cell-
                                                                        specific inhibitor of angiogenesis.




                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                        dues near the C-terminal of human PSA.
Paraffin-embedded human prostatic tissue stained with anti-PSA
RabMAb. Note positive staining of glandular epithelial cells




PUMA                                                               Cat #: 1652-1        Size: 100ul        min. 50 slides
                                                                                      Inquire for Bulk

   Type:        Rabbit Monoclonal IgG             Clone:         EP512Y            Species Reactivity: Human

IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic            Control:         Breast carcinoma,
                                                                 (Mitochondrion)                         Ovarian carcinoma


                                                                        Description: PUMA, (p53 upregulated modulator of apop-
                                                                        tosis), is activated by p53 in cells undergoing p53-induced
                                                                        apoptosis. Localized in the mitochondria PUMA induces
                                                                        cytochrome c release and activates the rapid induction of
                                                                        programmed cell death. PUMA interacts with Bcl2, Bcl-XL,
                                                                        Bcl2-like-1 protein and promotes mitochondrial transloca-
                                                                        tion and multimerization of BAX. PUMA also plays a role in
                                                                        mediating p53-induced cell death through the cytochrome
                                                                        c/APAF1-dependent pathway. Two isoform of PUMA are
                                                                        known; PUMA-alpha and PUMA-beta.




                                                                        Immunogen: A synthetic peptide corresponding to resi-
                                                                        dues in the C-term of human PUMA was used as immu-
Paraffin-embedded human ovarian adenocarcinoma tissue stained with      nogen.
anti-PUMA RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                       www.epitomics.com                  info@epitomics.com                       65
                                                                     info@biomol.de                  0800-2466651 www.biomol.de


                          Radixin                                                                   Cat #: 2042-1      Size: 100ul          min. 50 slides
                                                                                                                      Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         EP1862Y             Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                      Localization:      Cell Membrane,            Control:         Breast carcinoma
Primary Antibodies




                                                                                              Cytoplasmic


                                                                                                       Description: Radixin is a cytoskeletal protein that may be
                                                                                                       important in linking actin to the plasma membrane. Recent
                                                                                                       cloning of the murine and porcine radixin cDNAs revealed a
                                                                                                       protein highly homologous to ezrin and moesin. The ezrin-
                                                                                                       radixin-moesin (ERM) family of proteins crosslink actin fila-
                                                                                                       ments and integral membrane proteins. Radixin (encoded
                                                                                                       by Rdx) is the dominant ERM protein in the liver of wildtype
                                                                                                       mice and is concentrated at bile canalicular membranes
                                                                                                       (BCMs). Findings indicate that radixin is required for secre-
                                                                                                       tion of conjugated bilirubin through its support of Mrp2
                                                                                                       localization at BCMs. Proteins of the ezrin-radixin-moesin
                                                                                                       family are ubiquitous constituents of the submembrane cor-
                                                                                                       tex, especially in epithelial cells. Radixin is thus a prominent
                                                                                                       constituent of stereocilia, where it may participate in anchor-
                                                                                                       ing the pointed ends of actin filaments to the membrane.

                                                                                                       Immunogen: A synthetic peptide corresponding to resi-
                                                                                                       dues near the N-terminus of human Radixin was used as
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue          an immunogen.
                          stained with anti-Radixin RabMAb. Note positive staining of tumor cells




                          Raf-1                                                                     Cat #: 1560-1      Size: 100ul          min. 50 slides
                                                                                                                      Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         Y198                Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 200                      Localization:      Cytoplasmic,              Control:         Tonsil
                                                                                              Nucleus


                                                                                                       Description: Raf-1 (c-Raf) is a protein kinase that is in-
                                                                                                       volved in the transduction of mitogenic signals from the cell
                                                                                                       membrane to the nucleus. Raf-1 is recruited by and binds to
                                                                                                       GTP-bound Ras, activating the MEK-MAP kinase pathway.
                                                                                                       Raf-1 is activated via rapid hyperphosphorylation at multiple
                                                                                                       sites in mammalian cells, including Ser43, Ser259, and
                                                                                                       Ser621, as well as Ser338, Tyr341, Thr491, and Ser494.
                                                                                                       p21-activated protein kinase (PAK) phosphorylates Raf-1
                                                                                                       at Ser338 in response to Ras, Src and epidermal growth
                                                                                                       factor.




                                                                                                       Immunogen: A synthetic peptide corresponding to resi-
                                                                                                       dues of human Raf-1 was used as immunogen.
                          Paraffin-embedded human breast ductal infiltrating carcinoma tissue
                          stained with anti-Raf-1 RabMAb. Note positive staining of tumor cells




                     66    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                          info@biomol.de                 0800-2466651 www.biomol.de


Retinoblastoma-binding protein 4                                        Cat #: 2599-1       Size: 100ul         min. 50 slides
                                                                                           Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EPR3412             Species Reactivity: Human




                                                                                                                                                   Primary Antibodies
IHC Dilution:   1:250 - 500                     Localization:     Nuclear                   Control:          Breast Carcinoma



                                                                            Description: Retinoblastoma binding protein 4 (RBBP4,
                                                                            RbAp48) is an ubiquitously expressed nuclear protein and
                                                                            a core histone-binding subunit. RBBP4 target chromatin
                                                                            assembly factors, chromatin remodeling factors and histone
                                                                            deacetylases. RBBP4 is involved in histone acetylation and
                                                                            chromatin assembly. RBBP4 is expressed in several cel-
                                                                            lular proteins which binds directly to retinoblastoma protein
                                                                            to regulate cell proliferation. RBBP4 has been identified
                                                                            as a critical mediator controlling the transforming activity
                                                                            of human papillomavirus type 16 in cervical cancer and a
                                                                            targeting factor of (NF)-kappaB in thryoid cancer.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues in human RBBP4 was used as an immunogen.
Paraffin-embedded human breast ductal infiltrating carcinoma tissue
stained with anti-RBBP4 RabMAb. Note positive staining of tumor cells




S100 beta                                                               Cat #: 2017-1       Size: 100ul         min. 50 slides
                                                                                           Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:         EP1576Y             Species Reactivity: Human, Mouse, Rat

IHC Dilution:   1:100 - 250                     Localization:     Cytoplasmic               Control:          Melanomas



                                                                            Description: S100 beta is a homodimeric member of the
                                                                            EF-hand calcium-binding protein superfamily. The protein
                                                                            has been implicated in cellular processes such as cell dif-
                                                                            ferentiation and growth, plays a role in cytoskeletal structure
                                                                            and function, and may have a role in neuropathological dis-
                                                                            eases, such as Alzheimers. S100 beta is seen in ependy-
                                                                            momas, in most benign and malignant melanomas and their
                                                                            metastates, astrogliomas, and schwannomas.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues on the C-terminus of human S100 beta was used as
Paraffin-embedded human melanoma tissue stained with anti-S100 beta        an immunogen.
RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                        www.epitomics.com                     info@epitomics.com                         67
                                                                  info@biomol.de                 0800-2466651 www.biomol.de


                          Somatostatin receptor type 2                                          Cat #: 2600-1      Size: 100ul         min. 50 slides
                                                                                                                 Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:        EPR3507            Species Reactivity: Human

                          IHC Dilution:   1:250 - 500                    Localization:    Cytoplasmic,             Control:         Colon,
Primary Antibodies




                                                                                          Membrane                                  Neuroendocrine tumors


                                                                                                   Description: Regulatory peptide somatostatin (SRIF, STT)
                                                                                                   displays a broad tissue expression pattern. It modulates dif-
                                                                                                   ferent cell functions such as endocrine and exocrine secre-
                                                                                                   tions and proliferation. These actions have been described
                                                                                                   in glands and in the gastrointestinal and immune systems.
                                                                                                   They are mediated via six receptors (SSTR1, SSTR2A,
                                                                                                   SSTR2B, SSTR3, SSTR4, SSTR5). Recently published
                                                                                                   data point to the involvement of STTR2 receptor interaction
                                                                                                   in the control of proliferation of Sertoli cells and spermato-
                                                                                                   gonia.




                                                                                                   Immunogen: A synthetic peptide corresponding to resi-
                                                                                                   dues in human Somatostatin receptor type 2 / SSTR2 was
                          Paraffin-embedded human colon tissue stained with anti-SSTR2             used as an immunogen.
                          RabMAb. Note positive staining of glandular cells




                          ST13/HIP                                                              Cat #: 2552-1      Size: 100ul         min. 50 slides
                                                                                                                 Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG             Clone:        EP3264             Species Reactivity: Human, Mouse

                          IHC Dilution:   1:100 - 250                    Localization:    Cytoplasmic              Control:         Colon Adenocarcinoma



                                                                                                   Description: ST13/HIP is a cytoplasmic co-chaperone in-
                                                                                                   volved in the regulation of Hsc70 chaperone activity and the
                                                                                                   maturation of progesterone receptor. The expression level
                                                                                                   of ST13 gene is significantly reduced in colorectal tumors.




                                                                                                   Immunogen: A synthetic peptide corresponding to resi-
                                                                                                   dues near the C-terminus of human ST13/HIP was used
                          Paraffin-embedded human normal colon tissue stained with anti-ST13/      as an immunogen.
                          HIP RabMAb. Note positive staining of glandular cells




                     68    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                           info@biomol.de                  0800-2466651 www.biomol.de


Stathmin                                                               Cat #: 1972-1         Size: 100ul          min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EP1573Y              Species Reactivity: Human, Mouse, Rat




                                                                                                                                                    Primary Antibodies
IHC Dilution:   1:250 - 500                      Localization:     Cytoplasmic                Control:         Breast carcinomas



                                                                             Description: Stathmin (oncoprotein 18, op18) is a ubiq-
                                                                             uitous cytosolic phosphoprotein with various regulatory
                                                                             functions in cell proliferation, differentiation signaling, and
                                                                             activation. In particular, stathmin is involved in the regula-
                                                                             tion of tubulin dynamics through inhibition of microtubule
                                                                             formation and/or microtubule depolymerization. Stathmin
                                                                             interacts with soulable tubulins resulting in the formation of
                                                                             T2S complex which sequesters free tubulin, impeding tu-
                                                                             bulin formation. A mutation on stathmin can lead to excess
                                                                             build up of mitotic spindle and with possible consequence
                                                                             of unregulated cell cycles seen in cancer cells. Stathmin-1
                                                                             expression has a prognostic value in ER-positive primary
                                                                             breast cancer patients.




                                                                            Immunogen: A synthetic peptide corresponding to
                                                                            residues in C-terminus of human Stathmin was used as
Paraffin-embedded human breast ductal infiltrating carcinoma tissue         immunogen.
stained with anti-Stathmin RabMAb. Note positive staining of tumor cells




Survivin                                                               Cat #: 2463-1         Size: 100ul          min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG               Clone:         EP2880Y              Species Reactivity: Human

IHC Dilution:   1:250 - 500                      Localization:     Nuclear                    Control:         Colonic adenocarcinoma,
                                                                                                               Brain


                                                                             Description: Survivin is a unique member of the inhibitor of
                                                                             apoptosis (IAP) protein family that interferes with post-mito-
                                                                             chondrial events including activation of caspases. Survivin
                                                                             regulates cell cycle and is expressed in most tumors, but it
                                                                             is barely detectable in the terminally differentiated normal
                                                                             cells and tissues. The differential expression of survivin in
                                                                             cancer versus normal tissues makes it a useful tool in can-
                                                                             cer diagnosis and a promising therapeutic target. Survivin
                                                                             is expressed in the G2/M phase of the cell cycle. At the
                                                                             beginning of mitosis, survivin associates with microtubules
                                                                             of the mitotic spindle in a specific and saturable reaction
                                                                             that is regulated by microtubule dynamics. Disruption of
                                                                             survivin-microtubule interactions results in loss of survivin’s
                                                                             anti-apoptosis function and increased caspase-3 activity, a
                                                                             mechanism involved in cell death, during mitosis.

                                                                            Immunogen: A synthetic peptide corresponding to resi-
                                                                            dues on the N terminus of human Survivin was used as
Paraffin-embedded human colonic adenocarcinoma tissue stained with          an immunogen.
anti-Survivin RabMAb. Note positive staining of tumor cells




 Toll Free : 1-877-7RABMAB (772-2622)                         www.epitomics.com                      info@epitomics.com                        69
                                                                    info@biomol.de                   0800-2466651 www.biomol.de


                          Synaptophysin                                                             Cat #: 1485-1       Size: 100ul          min. 50 slides
                                                                                                                       Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         YE269                Species Reactivity: Human, Mouse, Rat

                          IHC Dilution:   1:100 - 250                      Localization:      Cytoplasmic                Control:         Pancreas
Primary Antibodies




                                                                                                        Description: Synaptophysin (SypI), also referred as p38,
                                                                                                        constitutes approximately 7% of the total vesicle protein
                                                                                                        and is selectively associated with small synaptic vesicles
                                                                                                        (SSVs) Syp I contains four transmembrane regions and can
                                                                                                        be phosphorylated on its cytoplasmic C-terminus by both
                                                                                                        serine/threonine and tyrosine kinases. In addition to syp I,
                                                                                                        synaptic vesicles contain a highly homologous membrane
                                                                                                        protein named synaptoporin or synaptophysin II (Syp II).
                                                                                                        While Syp I is ubiquitously expressed and present in virtu-
                                                                                                        ally all synapses whereas Syp II is expressed at high levels
                                                                                                        only in selected neurons. Synaptophysin expression is
                                                                                                        seen in a wide range of neuroendocrine tumors such as
                                                                                                        neuro-blastomas and ganglioneuroblastomas.




                                                                                                       Immunogen: A synthetic peptide corresponding to
                                                                                                       residues in C-terminus (cytoplasmic domain) of human
                          Paraffin-embedded human pancreas tissue stained with anti-Synapto-           Synaptophysin was used as immunogen. Predicted to
                          physin RabMAb. Note positive staining of secretory cells in the Langer-      cross-react with bovine, based on sequence homology.
                          hans islets




                          TCF-1 alpha / LEF-1                                                       Cat #: 2458-1       Size: 100ul          min. 50 slides
                                                                                                                       Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG                Clone:         EPR2029Y             Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                      Localization:      Nuclear                    Control:         Thyroid



                                                                                                        Description: The human T cell-specific transcription factor
                                                                                                        TCF-1 alpha/LEF-1 plays a key role in the tissue-specific
                                                                                                        activation of the T cell receptor (TCR) alpha enhancer and
                                                                                                        binds to pyrimidine-rich elements (5’-PyCTTTG-3’) present
                                                                                                        in a variety of other T cell-specific control regions (1). TCF-1
                                                                                                        alpha/LEF-1participates in the regulation of TCR alpha
                                                                                                        enhancer by facilitating the assembly of multiple proteins
                                                                                                        into a higher order nucleoprotein complex. The function of
                                                                                                        TCF-1 alpha/LEF-1 is dependent, in part, on the HMG do-
                                                                                                        main that induces a sharp bend in the DNA helix, and on an
                                                                                                        activation domain that stimulates transcription only in a spe-
                                                                                                        cific context of other enhancer-binding proteins. Constitutive
                                                                                                        activation of the Wnt signaling pathway is a root cause of
                                                                                                        many colon cancers.


                                                                                                       Immunogen: A synthetic peptide corresponding to
                                                                                                       residues in human TCF-1 alpha/LEF-1 was used as an
                          Paraffin-embedded human tonsil tissue stained with anti-TCF-1 alpha/         immunogen.
                          LEF-1 RabMAb. Note positive staining of T lymphocytes




                     70    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                           info@biomol.de                0800-2466651 www.biomol.de


Thyroglobulin                                                           Cat #: 2635-1        Size: 100ul          min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG                Clone:         EPR3614             Species Reactivity: Human




                                                                                                                                                   Primary Antibodies
IHC Dilution:   1:100 - 250                      Localization:      Membrane,                 Control:         Thyroid carcinoma
                                                                    Cytoplasmic


                                                                              Description: Thyroglobulin (Tg) is a large glycoprotein
                                                                              specific to the thyroid gland and is the precursor of the
                                                                              iodinated thyroid hormones thyroxine (T4) and triiodothyro-
                                                                              nine (T3). The N-terminal section of Tg contains 10 repeats
                                                                              of a domain of about 65 amino acids which is known as the
                                                                              Tg type-1 repeat. Such a domain has also been found as
                                                                              a single or repeated sequence in the HLA class II associ-
                                                                              ated invariant chain; human pancreatic carcinoma marker
                                                                              proteins.




                                                                           Immunogen: A synthetic peptide corresponding to resi-
                                                                           dues in human Thyroglobulin was used as an immuno-
Paraffin-embedded human papillary thyroid carcinoma tissue stained         gen.
with anti-Thyroglobulin RabMAb. Note positive staining of tumor cells




Topoisomerase II alpha                                                  Cat #: 1826-1        Size: 100ul          min. 50 slides
                                                                                            Inquire for Bulk

   Type:        Rabbit Monoclonal IgG                Clone:         EP1102Y             Species Reactivity: Human, Mouse

IHC Dilution:   1:100 - 250                      Localization:      Nuclear                   Control:         Tonsil



                                                                              Description: DNA topoisomerase II alpha (Topo II alpha) is
                                                                              a 170 kDa ubiquitous nuclear enzyme belonging to the topo
                                                                              family, which regulates the topological states of DNA. Topo
                                                                              II is required in chromatin condensation and segregation
                                                                              during mitosis. Topo II alpha is cell cycle regulated and its
                                                                              level peaks between G2 to M phase. It has been linked to
                                                                              cell proliferation and it may be the main isoform of Topo II
                                                                              involved mitotic processes. Topo II alpha passes one strand
                                                                              of DBA through a reversible break in a second DNA strand,
                                                                              which catalyzes the topological isomerisation of DNA during
                                                                              cell cycle. Topo II alpha overexpression has been linked to
                                                                              a number of human maligenacies and is the target for many
                                                                              chemotherapeutic agents. The majority of anticancer drugs
                                                                              targeting Topo II alpha initiate apoptosis by stabilizing the
                                                                              convalent complex formed between DNA and Topo II alpha.

                                                                           Immunogen: A synthetic peptide corresponding to C-
                                                                           terminal residues of human Topo II alpha was used as an
Paraffin-embedded human tonsil tissue stained with anti-Topo II alpha      immunogen.
RabMAb. Note positive staining of lymphocytes




 Toll Free : 1-877-7RABMAB (772-2622)                         www.epitomics.com                      info@epitomics.com                       71
                                                                    info@biomol.de                 0800-2466651 www.biomol.de


                          Transcription factor PU.1                                               Cat #: 2480-1        Size: 100ul          min. 50 slides
                                                                                                                      Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         EPR3158Y            Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                      Localization:     Nuclear                   Control:          Tonsil
Primary Antibodies




                                                                                                       Description: Transcription factor PU.1 is a member of the
                                                                                                       Ets family of transcription factors and is required for the
                                                                                                       development of multiple hematopoietic lineages. It plays a
                                                                                                       pivotal role in normal myeloid differentiation, and regulates
                                                                                                       the expression of immunoglobulin and other genes that are
                                                                                                       important for B-cell development. Transcription factor PU.1
                                                                                                       and AML1, a DNA-binding subunit of the CBF transcription
                                                                                                       factor family, act as tumor suppressors in leukemia. The
                                                                                                       lack of transcription factor PU.1 protein expression in classi-
                                                                                                       cal Hodgkin’s disease (cHD), a lymphoproliferative disease
                                                                                                       of predominantly B-cell origin, likely contributes to the lack
                                                                                                       of immunoglobulin expression and incomplete B-cell pheno-
                                                                                                       type characteristic of the Reed-Sternberg cells in cHD.




                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near the N-terminus of Transcription factor PU.1 was
                          Paraffin-embedded human tonsil tissue stained with anti-Transcription      used as an immunogen.
                          factor PU.1 RabMAb. Note positive staining of B lymphocytes




                          Troponin 1                                                              Cat #: 1875-1        Size: 100ul          min. 50 slides
                                                                                                                      Inquire for Bulk

                             Type:        Rabbit Monoclonal IgG               Clone:         EP1106Y             Species Reactivity: Human

                          IHC Dilution:   1:100 - 250                      Localization:     Cytoplasmic               Control:          Cardic Muscle



                                                                                                       Description: Troponin I, one of 3 subunits that form the
                                                                                                       troponin complex of the thin filaments of striated muscle, is
                                                                                                       the inhibitory component of troponin, the thin filament regu-
                                                                                                       latory complex in striated muscle. Separate genes encode
                                                                                                       cardiac-specific fast and slow skeletal-specific isoforms of
                                                                                                       this protein. Unlike other contractile proteins, the cardiac
                                                                                                       isoform of troponin I (TnIc) is expressed only in cardiac
                                                                                                       muscle and therefore offers a model for cardiac-specific ex-
                                                                                                       pression. It is also subject to developmental regulation with
                                                                                                       increased expression occurring at the time of birth. TnIc,
                                                                                                       a sensitive and specific marker of myocardial cell injury,
                                                                                                       is useful in diagnosing and assessing prognosis in acute
                                                                                                       coronary syndromes. Studies report that TnIc is elevated in
                                                                                                       severe heart failure and may predict adverse outcomes.


                                                                                                     Immunogen: A synthetic peptide corresponding to resi-
                                                                                                     dues near the N-terminus of human Troponin 1 was used
                          Paraffin-embedded human heart tissue stained with anti-cardiac Tropo-      as an immunogen.
                          nin 1 RabMAb. Note positive staining of cardiac muscles




                     72    Complete datasheets and images for every antibody are available @ www.epitomics.com
                                         info@biomol.de              0800-2466651 www.biomol.de


Vimentin                                                           Cat #: 4211-1          Size: 100ul          min. 50 slides
                                                                                        Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:        SP20               Species Reactivity: Human, Mouse, Rat




                                                                                                                                                    Primary Antibodies
IHC Dilution:   1:200 - 400                    Localization:     Cytoplasmic              Control:          Sarcoma



                                                                         Description: Vimentin is the most common member of in-
                                                                         termediate filament (IF) family and one of the main compo-
                                                                         nents in cytoskeleton structure. It is expressed in during cell
                                                                         development and differentiation in variety of mescencymal
                                                                         cells and cell types derived from mesoderm. Vimentin is
                                                                         essential in the role of cell integrity and cytoskeletal stability.
                                                                         The reorganization of Vimentin, similar to all IF proteins, oc-
                                                                         curs during different stages of the cell cycle and cell signal-
                                                                         ing by a site-specific phosphorylation (serine and threonine
                                                                         residues). In particular, p21-activated kinase (PAK) phos-
                                                                         phorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which
                                                                         induces Vimentin specific reorganization. During cytoki-
                                                                         nesis, Vimentin is regulated by Rho-kinase (ROCK) and
                                                                         Aurora B through phosphorylation at Ser38 and Ser72.


                                                                         Immunogen: A recombinant human vimentin protein.

Immunohistochemical staining of paraffin-embedded Human melanoma
stained with anti-Vimentin




ZAP-70                                                             Cat #: 1491-1          Size: 100ul          min. 50 slides
                                                                                        Inquire for Bulk

   Type:        Rabbit Monoclonal IgG              Clone:        YE291              Species Reactivity: Human

IHC Dilution:   1:100 - 250                    Localization:     Cytoplasmic              Control:          Tonsil



                                                                         Description: ZAP-70, a Syk-family protein tyrosine kinase,
                                                                         plays a critical role in mediating T cell signal transduction in
                                                                         response to T cell receptor (TCR) activation . TCR-mediat-
                                                                         ed activation of the Src-family kinases, Lck and Fyn, results
                                                                         in tyrosine phosphorylation of the TCR zeta and CD3
                                                                         chains. These domains serve as targets for binding of ZAP-
                                                                         70 via its tandem SH2 domains. This binding correlates
                                                                         with activation of ZAP-70, a critical event in T cell activation.
                                                                         Following TCR engagement, ZAP-70 is phosphorylated on
                                                                         several tyrosine residues, presumably by two mechanisms:
                                                                         an autophosphorylation and a trans-phosphorylation by the
                                                                         Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319
                                                                         is required for full activation and increased positive down-
                                                                         stream regulation by ZAP-70.


                                                                         Immunogen: A synthetic peptide corresponding to
                                                                         residues in C-terminus of human ZAP-70 was used as
Paraffin-embedded human tonsil tissue stained with anti-ZAP-70           immunogen.
RabMAb. Note positive staining of T lymphocytes




 Toll Free : 1-877-7RABMAB (772-2622)                       www.epitomics.com                     info@epitomics.com                           73

				
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