Nordic immunohistochemical Quality Control Institute of Pathology, Aalborg Hospital, Ladegaardsgade 3, P.O.Box 561, DK-9100 Aalborg, Denmark Scheme 2007, Assessment Run 19 Aalborg, 22 January 2007 Dear participant Please find enclosed pairs of unstained sections according to your submitted protocols. If you are in any doubt, please read the details at the website: www.nordiqc.org, or send us an e-mail: email@example.com. Also inform us if slides are missing. Please note your three digit participant number (NQC…) shown on the address label and slides. This number must be indicated on all new slide labels and used for any communication with NordiQC. Stain the sections according to your protocol (only one protocol for each marker!), and return for each marker one stained slide together with your own control slide marked with your participant number to NordiQC at the above mentioned address not later than 7 February. Keep the remaining NordiQC slides as your control. The general results are communicated on the website about 1 April. Examples of optimal as well as insufficient stains will be illustrated (anonymously) and the corresponding methods described. The origin of recommended protocols giving optimal results is published, encouraging technicians and pathologists to communicate directly. About 7 April each laboratory will receive an e-mail with their individual assessment scores and (in case of borderline or poor marks) comments to the stains and suggestions for protocol improvement. Monographs describing the relevant epitopes and the expecting staining patterns are available on the website. Submitted sections are stored in the NordiQC file for future documentation. The laboratories may request their stained sections for review (in case their control set is lost). Best regards, NordiQC Mogens Vyberg Scheme manager Contents of the multitissue sections Top row closest to the glass label. CD23: CR: CyD1: Ki67: Podop: TTF1: 1. Tonsil fixed 4h, 1. Appendix, 1. Tonsil fixed 4h, 1. B-CLL, 1. Ovarian clear cell 1. Lung 2. Tonsil fixed 72h, 2. Kidney, 2. Tonsil fixed 72h, 2. Burkitt carcinoma, adenocarcinoma, 3. Tonsil fixed 3. Malignant 3. Tonsil fixed lymphoma, 2. Ovarian serous 2. Lung carcinoid, 168h, mesothelioma, 168h, 3. Tonsil fixed 24h, carcinoma, 3. Lung, 4. + 5. B-CLL, epithelioid, 4. B-CLL, 4. Tonsil fixed 72h, 3. Appendix, 4. Thyroid gld., 6. Mantle cell 4. Malignant 5. + 6. Mantle cell 5. Tonsil fixed 4. Seminoma, 5. Liver. lymphoma. mesothelioma, lymphoma. 168h. (4 and 5 may 5. Embryonal biphasic, contain little or no carcinoma 5. Ovarian lymphoid tissue). 6. Malignant granulosa cell mesothelioma, tumor. epithelioid.
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