Docstoc

Cover page to Table of Contents_ Abstract__

Document Sample
Cover page to Table of Contents_ Abstract__ Powered By Docstoc
					A PRELIMINARY STUDY ON THE POTENTIAL BIODEGRADING ABILITY OF
Xylaria sp. ON NATURAL RUBBER, CHICKEN FEATHER, AND POLYSTYRENE
               THROUGH SCANNING ELECTRON MICROSCOPY




                     Janine Erica Padilla Dayao
                   Mary Bernadette Vallesfin Egloso




                            Submitted to the
                        Department of Biology
                     College of Arts and Sciences
                  University of the Philippines Manila
                   Padre Faura St., Ermita, Manila




                      In partial fulfillment of the
                           For the degree of
                    Bachelor of Science in Biology
                              March 2009
                                                      A Preliminary Study on the Potential……..ii
                                                                         Dayao and Egloso, 2009

                               BIOGRAPHICAL DATA


Name:               Mary Bernadette V. Egloso
Nickname:           Addie, Madette
Birthday:           June 20, 1988
Address:            135 Phase 4 Block 5 Lot 18, Our Lady of Lourdes Avenue., V&G
                    Subdivision, Tacloban City, Leyte

Telephone Number: (053) 327 – 3714

Mobile Number:      09153831788

E-mail Address:     adelaide17madette@yahoo.com

Parents:            Efren P. Egloso
                    Salve V. Egloso

Sibling:            Mary Jeane V. Egloso


Educational Background:
       Level                   School               Years Attended        Recognition
    Pre-School      United Church Family Life        1993 – 1996         Honor Student
                  Program Pre-Elementary School
     Primary          Sto. Niño SPED Center          1996 – 2001           Salutatorian
      School
    Secondary     Philippine Science High School     2001 – 2005
      School         – Eastern Visayas Campus
     College        University of the Philippines    2005 – 2009        College Scholar
                               Manila


Organizations/Affiliations:
      Member, UP Manila Chorale
      Member, Youth for Christ, UP Manila Chapter
      Member, UP Biological Sciences Society
      Member, UP Manila Quod Erat Demonstrandum
      Member, Biology Majors Association
                                                    A Preliminary Study on the Potential……..iii
                                                                        Dayao and Egloso, 2009

                                BIOGRAPHICAL DATA


Name:                Janine Erica P. Dayao

Nickname:            Jana

Birthday:            October 4, 1988

Address:

Telephone Number:

Mobile Number:

E-mail Address:      jana_erica99@yahoo.com

Parents:

Sibling:


Educational Background:
             Level                School      Years Attended         Recognition
          Pre-School                           1993 – 1996
        Primary School                         1996 – 2001
       Secondary School                        2001 – 2005
            College                            2005 – 2009


Organizations/Affiliations:

        Member, UP Biological Sciences Society
        Member, UP Manila Quod Erat Demonstrandum
        Member, Biology Majors Association
                                              A Preliminary Study on the Potential……..iv
                                                                 Dayao and Egloso, 2009

                                 ACKNOWLEDGMENTS



GOD:



Our Parents:



Asst. Prof. Anna Theresa Santiago:



Dr. Mary Ann Tavanlar:



Prof. Jay Dalet:



Prof. Kimberly Beltran:



Asst. Prof. Mark Romano:



UST and de La Salle Libraries:



Blockmates and Friends:
                                                             A Preliminary Study on the Potential……..v
                                                                                Dayao and Egloso, 2009




                                           ABSTRACT


         The potential biodegrading ability of the Xylaria sp. strains on three pollutants, namely,
natural rubber, chicken feather and polystyrene were determined in terms of colonization. The
fungal strains namely the wild type, SDM (sterile dark mycelia) and the five mutant strains (PNL
114, PNL 116, PNL 118, E26 and E35) were cultured in a pH 5.0 mineral medium with 0.5%
glucose as carbon source, while the 3 pollutant substrates as co-carbon source, and stored at
250C. The initial weights of the pollutants were measured before inoculation. After 50 days of
incubation, the pollutants were removed from the medium and weights were again measured to
determine percent weight loss, a method that would measure utilization of the pollutant samples.
But instead of constant weight loss, weight gains resulted, which could be attributed to the
adherence and penetration of mycelia of the Xylaria sp. strains in the three pollutant substrates.
Scanning Electron Microscopy (SEM) revealed colonization of Xylaria sp. strain on natural
rubber, chicken feather and polystyrene. Generally, the black strains, namely the SDM wild type,
E26 and E35, have been observed to possess a higher potential to biodegrade natural rubber,
chicken feather and polystyrene than the albino mutants PNL 114, 116 and 118. Among the
black variant strains, E26 is the best colonizer for natural rubber, whereas the albino mutants also
showed potential ability to biodegrade this substrate. On the other hand, the three black strains,
SDM wild type, E26 and E35, were comparably the better colonizers for polystyrene than the
albino mutants, but nonetheless they also showed potential ability to biodegrade this substrate.
Lastly, no significant changes occurred to the chicken feather samples due to the short incubation
period. However, SDM and E35 black strains have shown to colonize chicken feather.
                                 A Preliminary Study on the Potential……..vi
                                                    Dayao and Egloso, 2009

                  TABLE OF CONTENTS

TITLE PAGE…………….………………………………………………………………… i

BIOGRAPHICAL DATA………………………………………………………………… ii

ACKNOWLEDGMENTS………………………………………………………………… iv

TABLE OF CONTENTS………………………………………………….……………….                         vi

LIST OF FIGURES…………………………………………………………………………..                             vii

LIST OF TABLES…………………………………………………………………………                           viii

LIST OF APPENDICES……………………………………………………………………..                             ix

ABSTRACT………………………………………………………………………………….                                   x

INTRODUCTION…………………………………………………………………………...                               1

REVIEW OF RELATED LITERATURE………………………………………………………

MATERIALS AND METHODS……………………………………………………………….

RESULTS……………………………………………………………………………………….

DISCUSSION…………………………………………………………………………………...

CONCLUSION……………………………………………………………………….…………

RECOMMNEDATIONS……………………………………………………………………….

LITERATURE CITED………………………………………………………………………….

APPENDIX……………………………………………………………………………………...
                                                     A Preliminary Study on the Potential……..vii
                                                                         Dayao and Egloso, 2009

                                  LIST OF FIGURES



FIGURE                                                                                 PAGE

 1. Scheme for Polymer Biodegradation………………………………………………………..

 2. Natural rubber is a polymer called polyisoprene…………………………….………....……

 3. Gallus sp. is the source of feathers for the current study……………....……………………

 4. Polystyrene…………………………………………………………………………………..

 5. Growth of Xylaria sp. in mineral medium with 0.5% glucose and natural rubber…………..

 6. SEM of the non-inoculated natural rubber control without the fungus……………………...

 7. SEM of natural rubber surface colonized by the SDM or wild type strain
     after 50 days of incubation………………………………………………………………...

 8. SEM of natural rubber surface colonized by the PNL 114 mutant strain
     after 50 days of incubation………………………………………………………………...

 9. SEM of natural rubber surface colonized by the PNL 116 mutant strain
     after 50 days of incubation………………………………………………………………..

 10. SEM of natural rubber surface colonized by the PNL 118 mutant strain
      after 50 days of incubation……………………………………………………………….

 11. SEM of natural rubber surface colonized by the E26 mutant strain
      after 50 days of incubation……………………………………………………………….

 12. SEM of natural rubber surface colonized by the E35 mutant strain
      after 50 days of incubation……………………………………………………………….

 13. Growth of Xylaria sp. in mineral medium with 0.5% glucose and chicken feather……….

 14. SEM of non-inoculated chicken feather control without the fungus………………………

 15. SEM of chicken feather colonized by the SDM wild type strain
      after 50 days of incubation……………………………………………………………….

 16. SEM of chicken feather colonized by the PNL 114 mutant strain
      after 50 days of incubation……………………………………………………………….
                                                  A Preliminary Study on the Potential……..viii
                                                                       Dayao and Egloso, 2009

17. SEM of chicken feather colonized by the PNL 116 mutant strain
     after 50 days of incubation……………………………………………………………….

18. SEM of chicken feather colonized by the PNL 118 mutant strain
     after 50 days of incubation……………………………………………………………...

19. SEM of chicken feather colonized by the E26 mutant strain
     after 50 days of incubation……………………………………………………………...

20. SEM of chicken feather colonized by the E35 mutant strain
     after 50 days of incubation……………………………………………………………...

21. Growth of Xylaria sp. in mineral medium with 0.5% glucose and polystyrene………….

22. SEM of non-inoculated control sample…………………………………………………..

23. SEM of polystyrene surface colonized by the SDM wild type strain
     after 50 days of incubation……………………………………………………………..

24. SEM of polystyrene surface colonized by the PNL 114 mutant strain
     after 50 days of incubation……………………………………………………………..

25. SEM of polystyrene surface colonized by the PNL 116 mutant strain
     after 50 days of incubation……………………………………………………………..

26. SEM of polystyrene surface colonized by the PNL 118 mutant strain
     after 50 days of incubation……………………………………………………………..

27. SEM of polystyrene surface colonized by the E26 mutant strain
     after 50 days of incubation……………………………………………………………..

28. SEM of polystyrene surface colonized by the E35 mutant strain
     after 50 days of incubation……………………………………………………………..
                                                     A Preliminary Study on the Potential……..ix
                                                                        Dayao and Egloso, 2009

                                  LIST OF TABLES



TABLE                                                                                 PAGE

 1. Comparative growth of the PNL mutants and wildtype SDM on MMG
       and mineral medium with various supplements. …………………………………….…

 2. Growth of the four albino mutants on mineral medium with and without
     supplements as compared to the wildtype SDM………………………………...…..……

 3. Growth of the two black mutants on mineral medium with supplements
       MDPE and PEG……………………………………………………………..….………

 4. Table of the Macroscopic/Physical and Microscopic Observations of the Xylaria sp.
       Mutant Strains and Wild Type on Colonized Natural Rubber after 50 days of
       Incubation…………………………………………………………………….………..

 5. Table of the Macroscopic/Physical and Microscopic Observations of the Xylaria sp.
       Mutant Strains and Wild Type on Colonized Chicken Feather after 50 days of
       Incubation…………………………………………………………………….………..

 6. Table of the Macroscopic/Physical and Microscopic Observations of the Xylaria sp.
       Mutant Strains and Wild Type on Colonized Polystyrene after 50 days of
       Incubation…………………………………………………………………….………..

				
DOCUMENT INFO
Categories:
Stats:
views:0
posted:6/27/2011
language:English
pages:9
Mary Bernadette Vallesfin Egloso Mary Bernadette Vallesfin Egloso English Teacher http://adelaide17madette.multiply.com/
About My friends call me Addie. I want to become a doctor someday and serve my countrymen after studying medicine in the Philippines. I also want to become a sophisticated investor and business owner someday. I truly believe in what Robert Kiyosaki said in his books. It is very important to keep on improving oneself, as we live in this dynamic and competitive world. I love swimming and singing.