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					Programme: Medical Lab Technology
Course code: D105


Programme                    Duration                    Medium                       Eligibility

Medical Lab                  1 year                      English                      10+2 0r Equivalent
Technology




S.no       Code            Course Name                                                           syllabus



1          DMLT-1          Human anatomy, physiology and clinical bio-                           Available
                           chemistry
2          DMLT-2          Clinical pathology & histo pathology                                  Available
3          DMLT-3          Lab instruments and ethics                                            Available
4          DMLT-4          Micro-biology and serology                                            Available
5          DMLT-5          Haemotology and blood banking                                         Available
6          DMLT-6          Record work & practical




                           PAPER-I- HUMAN ANATOMY & PHYSIOLOGY


HUMAN ANATOMY & PHYSIOLOGY:-
UNIT I
          Cell structure, division & function - Cell organelles - Tissue: types of tissues and their functions -
Skeletal system.


UNIT II
          Digestive system: physiology and anatomy of mouth, stomach, intestine - Absorption of food and
its excretion - Role of bile in digestion and excretion - Liver function and a brief description of liver and
biliary tree.
UNIT III
         Respiratory system: brief description of larynx, bronchi, lungs - Cardiovascular system: anatomy
and physiology of heart, arteries and veins - Circulation: systematic and pulmonary (in brief) - Brief review
of chambers.


UNIT IV
         Urinary system: structure and function of the kidney, uterus, bladder, urethra and nephron
Give special emphasis on formation of urine - Physiology and anatomy of male and female reproductive
organs - Endocrine: pituitary, thyroid, parathyroid, thymus, adrenals and pancreas.


UNIT V
         Central nervous system: brain, spinal cord and meninges explain with its functions - Skins:
structure and functions - Study and give small project on bones and cartilage
HLA system.




CLINICAL BIOCHEMISTRY-II-

ch1: carbohydrates: carbohydrates metabolism, glycogenesis, glycolysis, regulation of
blood glucose concentration, diabetes mellitus, glycosuria, glucose tolerance test
ch2: lipids; introduction, simple lipids, compound lipids, derived lipids, essential fatty
acids, ketone bodies formation
ch3: proteins
definition, classification, structure of proteins, denaturation of proteins, important tests of
proteins, estimation of proteins, albumin, globulin, glycoproteins, protein estimation,
estimation of total proteins, albumin & globulin, estimation of blood urea, serum
creatinine estimation
serum uric acid estimation
ch4: liver function tests, serum bilirubin estimation, serum protein estimation
ch5: enzymology, introduction, definition & classification, properties, factors influencing
action of enzymes, diagnostic value of serum enzymes, mechanism of enzyme action
                                           DMLT-2
                CLINICAL PATHOLOGY AND HISTO PATHOLOGY




UNIT –I                           CLINICAL PATHOLOGY
               Introduction
               Urine analysis
               CSF
               Serous fluid
               Syndvical fluid
               Gastric juice
               Seminal analysis
               Sputum analysis




UNIT – II                HISTOPATHOLOGY & CYTOLOGY


               Introduction to histo technology
               Laboratory techniques in histology
               Introduction to cytology
Lab techniques in diagnostic cytology
                         LAB INSTRUMENTS AND ETHICS




UNIT – I                         LAB INSTRUMENTS


              Introduction to laboratory equipments and basic laboratory operations.
              Basic chemical / reagents
              Glassware
              Microscopes
              Software for laboratories
              Electronic transformers
              Incubator/oven/water bath / equipment for his to pathology
              Centrifuges
              Densitometry
              Disposable syringe and dr. snood’s multi purpose universal culture
               containers.
              Dipsticks for urine / glucostix/glucometer
              Blood group sera/antis era and blood bank production
              Pregnancy test kits
              Sterilization equipments
              Photo colorimeters / spectrophotometer meters
              Serum auto analysis
              Random across batch analysis
              Hematology counters
              Electrolyte controls
              Electrolyte analysis
              Coagulametry
              Bio chemical tests
              Elisa kit / sero immunology.
UNIT II                         LABORATORY ETHICS


              Organization of clinical laboratory and role of medical lab technician
              Safety regulations
              Quality control of lab findings
              Ethical issues in laboratory




UNIT III                   LABORATORY MANAGEMENT


              first aid and clinical lab records
              organization of lab
              introduction to lab equipments
              general comments and specimen collection
              units of measurements, preparation of reasons, solutions and lab
               calculations.
                  HAEMOTOLOGY AND BLOOD BANKING




UNIT I                                       INTRODUCTION TO HAEMATOLOGY


            Specimen collection and laboratory preparations in Hematology
            Routine hematological test
         a) HB estimation
         b) Total leukocyte estimation
         c) Total ery throcyte estimation
         d) Differential leulo cycte count
         e) ESR
         f) Packed cell volume
         g) Reticulocy count
         h) Plate lot count
         i) Absolute eosin Phil count
         j) Arneth count
         k) Schilling count
         l) Red cell indices
         m) Periphery cell – morphology


            Special Hematological test
         a) Foetal hemoglobin estimation
         b) Foetal red cell estimations
         c) Lupus ery thrematous cell preparation
         d) Osmotic fragility test
         e) Heinz bodies preparation
         f) Bone marrow smear preparations
         g) Study of blood parasites
             Interpretation of Lab findings in hematology anemia, leukemia, abnormal
              RBC and WSC morphology.
             Introduction to homeostasis, coagulation and bleeding disorders.
             Clotting and bleeding time determinations, coagulations mechanisms,
              coagulation studies.




UNIT II         BLOOD BANKING (IMMUNOHAEMATOLOGY)


             Principles of immunohae metrology, clinical significance of blood trans
              fusains.
             Collections and processing’s of blood for transfusion
             Routine lab procedures in blood bank
          a) ABO system
          b) Rh system
          c) Sub grouping
          d) Compatibility testing
          e) Coombs testing
          f) antibody titration and D’ testing
                        MICRO BIOLOGY AND SEROLOGY


UNIT I               INTRODUCTION TO MIKCRO BIOLOGY


              Historical survey
              Introduction to lab process
              Roll of medical micro biology
              Laboratory records
              Basic procedures of diagnostic micro biology
              Sterilization and disinfections
              Quality control




UNIT II    BACTERIOLOGY


              Systemic groups of pathogenic bacteria
              Lab identification of infections agents
              Diagnosis of anaerobic infections
              Identifying characteristics of common pathogenic bacteria
              Antimicrobiel syscebility test


UNIT III                              MICOLOGY


              Introduction and laboratory diagnosis of mitotic agents.


UNIT IV                             PARASITOLOGY
              Helminthological ( Nematode, cestuses and trematode)
              Proto zoology (Amoebas, Flagellates) ciliata
UNIT V                                VIROLOGY
             Introduction
             Classification of DNA & RNA virus
             Lab investigations and diagnosis
             Tissue culture, chick embryo culture, cell culture and anital inoculates.


UNIT VI                              SEROLOGY


             Introduction
             Principles of immunological agents
             Lab procedures in serology
             Collection and preparation and specimens
             Serological test = VDRL, WIDAL, CRP, R A, WFR, Brucellosis, ASO,
              HIV, HBs Ag, TPHA, Pregnancy, Rubella, Toxoplasmosis, etc.,