Protection of piglets against porcine epidemic diarrhea virus (PEDV) using sow oral administration
of cell culture-attenuated Thai PEDV
Mongkol Lumyai1, Porjit Choojai2, Roongroje Thanawongnuwech1 , Komkrich Teankum1
Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand
Veterinary Diagnostic Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand
Introduction Figure 2: Virus titers in gut of piglets challenged orally with
Porcine epidemic diarrhea virus (PEDV), a member of the virulent Thai PEDV.
Coronavirus, causes severe enteric disease especially in
suckling piglets with high mortality reaching up to 90%.
Similar to other viral enteritis virus, PEDV replicates in 5
diﬀerentiated enterocytes covering the villi of the small
intestine, leading to villous atrophy and malabsorption. 4
Recently, PEDV is one of the most important diseases
causing economic loss in Thailand and other Southeast 3 group1
Asian countries. The objective of this study was to study
the protection of piglets deriving from pregnant sows 2
receiving cell culture-attenuated Thai PEDV orally against
wild type PEDV challenge. 1
Materials and Methods
12 24 48 72 120
PEDV serial propagation in Vero cell passages 53
Hour post challenge
(orally inoculation 4 and 2 wk before farrowing) Results and Discussions
Pregnant sows inoculated with the attenuated Thai PEDV
Group 1; Orally inoculation Group 2; Control did not show any clinical signs of the disease or viral
(n=3) (n=1) shedding from fecal content. All piglets challenged with the
virulent Thai PEDV exhibited diarrhea within 48 hrs but
shown only 62% in the vaccinated group at 24 hrs after
- Clinical observation challenge (Fig. 1). Both groups yielded positive RT-PCR
- Detection of viral RNA detection in feces but the virus titers tested in the small
in feces using RT-PCR intestine of the control piglets had somewhat higher titers
than the piglets derived from vaccinated sows, particularly
at 48 hour post challenged (Fig. 2). It should be noted that
Piglets from immunized & non-immunized sow
the PEDV challenged dosage would have been higher
(challenged with wild-type PEDV)
resulting in more severe clinical disease than expected.
These results indicated that oral administration with the
cell culture-attenuated Thai PEDV in pregnant sows could
partially reduce clinical signs and virus shedding in piglets
Clinical Viral RNA detection in Virus titration; against virulence PEDV challenge.
observation feces: RT-PCR Small intestine
We would like to thank the staffs from Veterinary
Diagnostic Laboratory and Department of Veterinary
Protection of piglets from PEDV challenge Pathology, Faculty of Veterinary Science, Chulalongkorn
University for laboratory assistants and financial support
Figure 1: Percentage of diarrhea signs in piglets
from the National Research Council of Thailand.
challenged orally with the virulent Thai PEDV strain
1. De Arriba, M.L. et al. 2002. Vet. Immunol.
100 Immunopathol. 85, 85-97.
2. Kim, O. and Chae, C. 2003. J. Comp. Path. 129,
% Diarrhea sign
60 group1 3. Kweon, C.H. et al. 1999. Vaccine 17. 2546-2553.
group2 4. Song, D.S. et al. 2007. Res. In Vet. Science 82,
1 2 3 4 5
Day post challe nge
Proceedings of the 5th Asian Pig Veterinary Society Congress
7-9 March 2011, Pattaya, Thailand