O Lacham Dr Orly Lacham Kaplan by mikeholy

VIEWS: 79 PAGES: 30

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        CURRICULUM VITAE - ORLY LACHAM KAPLAN




              Email: orly.lacham-kaplan@med.monash.edu.au

                           Phone 61-425 725 649
                         In Australia: 0425 725 649


      Postal Address: POB 374, East Bentleigh, Victoria Australia, 3165



                               www.orly.com.au




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CURRENT POSITION HELD:

Senior Research Fellow (lecturer) Level C, Monash Immunology and Stem Cell
Laboratories, Monash University.

ACADEMIC BACKGROUND:

EDUCATION

            1990-1996:    Centre for Early Human Development, Faculty of
            Medicine, Monash University.

                            Ph.D. (P/T) "FERTILIZATION USING
            SPERM MICROINJECTION IN THE MOUSE AND IN THE
            HUMAN"

                           Supervisor: Prof. A. Trounson, Centre for Early
             Human Development, Institute for Reproduction and
             Development, Monash University.

            1987-1988:       Visiting scientist at the Centre for Early Human
            Development, development of mouse and human sperm
            microinjection techniques and formation of transgenic mice by
            nuclear injection of DNA.

            1984-1987:     Faculty of Life Science, Department of Zoology,
            Tel-Aviv University, M.Sc. (Biology) "THE TWO CELL BLOCK OF
            RAT PRE-IMPLANTATION EMBRYOS IN VITRO"

                                  Supervisors: Prof P.E. Kraicer, Zoology
            Department, Faculty of Life Science, Tel-Aviv University. Prof. R.
            Shalgi, Embryology Department, School of Medicine, Tel-Aviv
            University.

            1981-1983      Faculty of Life Science, Department of Zoology,
            Tel-Aviv University, B.Sc. (Biology)

SUPPORTIVE COURSES:

2003: “Frontiers in Human Embryonic Stem Cells” advanced training course at
the University of Pittsburgh, USA.



2003: DNA Techniques Course, Microbial Biotechnology & Diagnostic Unit, at
Monash University to expand my knowledge on basic molecular biology
including PCR, Western Blotting and Protein Immunoassay.


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2002: Leadership: Australian and International Perspectives (MGX5670),
Faculty of Business and Economics, Monash University

2001: Management Theory (MGX 5210), Faculty of Business and Economics,
Monash University

APPOINTMENTS:

            2005-current    Senior Research Fellow (C), Monash
            Immunology and Stem Cell Laboratories, Monash University.
            Supported by a RFA/NIH grant.

           2004-2005    Senior Research Fellow (C), Centre for Early
           Human Development, Institute of Reproduction and Development.

          2003 Teaching micromanipulation procedure in “Frontiers in
          Human               Embryonic Stem Cells” advanced training
          course at the University of Pittsburgh, USA.

            2000-2004 Research Fellow Level B, Centre for Early Human
           Development, Institute of Reproduction and development, in charge
           of mouse gamete manipulation laboratory

            2002-2003 Coordinator, lecturer and laboratory tutor: Master of
            Clinical Embryology (MCE): Practical Techniques & Statistics in
            Embryology 1 & 2, Monash University. Lecturer: Graduate Diploma
            of Reproductive Sciences, Monash University.

            1999-2001 Co-coordinator, lecturer and laboratory tutor: Master
            of Clinical Embryology (MCE): Practical Techniques & Statistics in
            Embryology 1 & 2. Monash University. Lecturer: Graduate Diploma
            of reproductive sciences, Monash University.

            1998-2000 Research Fellow Level A, Centre for Early Human
            Development, Institute of Reproduction and Development, in-
            charge of bovine gamete manipulation laboratory.

            1995-1997      Research Officer (RO2 to August 1996)/Research
            Fellow level A, Centre for Early Human Development, Institute of
            Reproduction and development, in-charge of mouse gamete
            manipulation laboratory.

            1993-1994     Chief Technical Officer, Centre for Early human
            Development, Institute for Reproduction and Development, in
            charge of micromanipulation laboratory.

            1989-1993     Senior Technical Officer, Centre for Early Human
            Development, Institute for Reproduction and Development, in
            charge of micromanipulation laboratory


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            1986-1987 IVF Technician, Infertility Service, Beilinson Medical
            Centre, in charge of quality control.

            1984-1987      Instructor in student laboratories: Comparative
            Anatomy, Histology and Endocrinology, Zoology Department, Tel-
            Aviv University.



            RESEARCH INTERESTS AND EXPERTISE:

            Research Interests:




         differentiation and maturation

                                                      tion in-vivo and in-vitro)



            -vitro fertilization

           Nuclear reprogramming




Technical experiences:



                                                                 supials, bovine,
         equine (high)

         * Nuclear injection with DNA constructs

         * Cell injection into mouse blastocysts to create chimeras

         * Sperm injection



            -vitro fertilization in humans (average) and mice (high)

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           and mouse (high)




             erm cell transplantation




OUTSTANDING ACHIEVEMENTS:

Public Awards:

                                                  n of Achievement Award.

Scientific breakthroughs:


       sperm into aspirated oocytes.

Orly Lacham-Kaplan developed a unique method to treat horse sperm
following freezing and when used for ICSI, viable embryos were produced.
The embryos were transferred to recipient females resulting in the birth of 2
healthy foils. These foils were the first in the world to be produced from a
frozen sperm used for ICSI in horses. The breakthrough was the elimination of
large amount of sperm to be used for artificial fertilization in equine breeding.
Hence cost related inseminations could be reduced to minimum. This
breakthrough was not, however, welcomed by the breeding industry as it
reduces profit dramatically.

Related publication: A. McKinnon, O. Lacham-Kaplan, A. Trounson (2000):
Pregnancies produced from fertile stallions by intracytoplasmic sperm
injection (ICSI) of single frozen/thawed spermatozoa into in-vivo matured male
oocytes. J. Reprod. Fert. Supp. 56: 513-517.

       *



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Orly Lacham-Kaplan and her team produced NT embryos from oocytes
obtained from slaughter house material. The oocytes were matured in vitro
and used for NT. Embryos produced were transferred to recipient females and
these transfers resulted in the birth of the first 2 cloned calves in Australia.

Related publication: O. Lacham-Kaplan, M. Diamente, D. Pushett, V. Hall, I.
Lewis and A. Trounson (2000): Developmental competence of nuclear
transfer cow oocytes following direct injection of fetal fibroblast nuclei.
Cloning: 2:2.

       *

Orly Lacham-Kaplan used somatic cells to fertilize mouse oocytes. Male and
female cells were injected into mature oocytes and by inducing haploidization
morphologically normal embryos were produced. The importance of this
achievement is that somatic cells follow the steps of diploid germ cells. The
somatic cell ignores its own cleavage cycle checkpoints and enters semi-
meiosis. The other impact of this achievement is on fertility. If the embryos
were found viable than the procedure could be applied in human as ART.
Hence giving an opportunity to many sterile men to reproduce and also an
opportunity for two women to reproduce. The study received worldwide
exposure.

Related publication: O. Lacham-Kaplan, R. Daniels, A. Trounson (2001)
Fertilization of mouse oocytes using somatic cells as male germ cells.
Reproductive Biomed. Online, 3:205-211.



Orly Lacham-Kaplan used embryonic stem cells to obtain germ cells in-vitro
following the establishment of embryoid bodies. Embryoid bodies cultured in
testicular cell conditioned medium turned into structures resembling ovaries.
The periphery of these ovaries was structured with follicles that contained
immature oocytes.

Related Publication: Orly Lacham-Kaplan, Hun Chy and Alan Trounson
(2006): Testicular cell conditioned medium supports differentiation of
embryonic stem cells into ovarian structures containing oocytes. Stem Cells
24: 266-273.

GRANT MONIES and AWARDS:

Teaching Awards:

1999-2002: AU$6,000 for coordinating the MCE1100/1200 and for lecturing in
the Graduate Diploma in Reproductive Sciences course.

Research Grants:



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2007-2007: Faculty of Medicine, Nursing and Health. Differentiation of
embryonic stem (ES) cells to the female germ line CI: Orly Lacham-Kaplan.
$10,000

 2007-2007: Monash Research Fund (MRF). Differentiation of embryonic stem
(ES) cells to the female germ line CI Orly Lacham-Kaplan. $10,000

2003 to 2008: NICHD/RFA Female health and egg quality. PCI: Alan
Trounson,

Partner Investigators: Findlay Jock, Graham Jenkin,Gayle Jones, Orly
Lacham-Kaplan. AU$ 2,000,000

 2003-2004: Monash IVF: In-vitro production of male germ cells (GC) from
embryonic stem (ES) cells. CI: Orly Lacham-Kaplan. CoI: Alan Trounson.
AU$100,000

 2000-2003: NICHD/ RFA: Cryopreservation of ejaculated and epididymal
sperm for mouse mutants and analyses of the safety of such methodology.
PCI: Alan Trounson. Partner I: Paul Hertzog, Jill Shaw, Orly Lacham-Kaplan.
AU$2,496,805.


2000: Monash IVF: “Fertilization using somatic cells as artificial gametes”. CI:
Orly Lacham-Kaplan. CoI: Rob Daniels: AU$12,000.

 1999-2000: Significant Monash University Research Fund (SMURF): Nuclear
cloning of fetal and adult somatic cells of rats. CI: Alan Trounson, Eric Hayes,
Orly Lacham-Kaplan, John Morison, Martin Pera. AU$168,000.

 1999-2001: DRDCMRC: The production of Elite breeding Stock by Nuclear
transfer and embryo multiplication. PCI: Alan Trounson, Chris Langford. CI: Kurt
Zuelk, Ian Lewis, Orly Lacham-Kaplan, Teija Peura. AU$ 746,028



1999: Monash University Research Fund (MURF): Nuclear cloning of terminally
differentiated fetal and adult somatic cells of mice. CI: Alan Trounson, Orly
Lacham-Kaplan. AU$35,000.

1998: Monash IVF: Chromosome analyses of human sperm and spermatids
using fluorescent in situ hybridisation (FISH) techniques. CI: Orly Lacham-
Kaplan. Co-I: Alan Trounson; Robert McLachlan, Lyn Grass. AU$68,000.

1998: MIRD Infrastructure grant: CI: Orly Lacham-Kaplan AU$ 8,500

1997: Monash IVF: Injection of human sperm into mouse oocytes for the
purpose of chromosomal and centrosomal analysis. CI: Orly Lacham-Kaplan,
Michael Herbert Co-I: Henry Sathananthan, Alan Trounson, AU$19,000.


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1996: Monash-IVF: Sperm cytosolic factor- its identification and participation
in oocyte activation and fertilization. CI: Orly Lacham-Kaplan; Yoshinori
Ohkuma; Alan Trounson, AU$25,000.

1996: Monash IVF: Effect of sperm maturity and methylation state on embryo
viability CI: Margaret Jones; Orly Lacham-Kaplan. AI: Alan Trounson; Robert
McLachlan, AU$39,000.

1996: Monash University Travel Grant to the ESHRE annual meeting,
Netherland: AU$1,000

PATENTS

                                                     (international filing) –
         PCT/AU99/00275

   This patent was supported by Genetics Australia, MIRD and Monash
   University. The patent is related to the use of “naked” somatic cell nuclei
   for NT by injection. A publication related to this patent is:

    O. Lacham-Kaplan, M. Diamente, D. Pushett, V. Hall, I. Lewis and A.
   Trounson (2000): Developmental competence of nuclear transfer cow
   oocytes following direct injection of fetal fibroblast nuclei. Cloning: 2:2.

  
        producing cloned cells or genetically modified animals- PQ

   This patent was supported by MIRD and Monash University. The patent is
   related to methods of nuclear transfer in the rat. A publication related to
   this patent is:

   E. Hayes, S. Galea, A. Verkuylen , M. Pera, J. Morrison, O. Lacham-
   Kaplan, A. Trounson (2001): Nuclear transfer of normal and genetically
   modified somatic cells in the rat. Physiological Genomics 5: 193-203.

      * April 2000: Fertilization by somatic cells (international filing) – PQ8001

   The patent was submitted in Australia in 2000 and in the USA in 2001 and
   was supported by M-IVF, MIRD and Monash University. The patent is
   related to induction of haploidization of somatic cells in mouse oocytes for
   the purpose of fertilization. A publication related to this patent is:

   O. Lacham-Kaplan, R. Daniels, A. Trounson (2001) Fertilization of mouse
   oocytes using somatic cells as male germ cells. Reproductive Biomed.
   Online, 3:205-211.




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All patents lapsed by March 2003.

CLINICAL INVOLVEMENT:



1986-1987: IVF Technician, Infertility Service, Beilinson Medical Centre:

       -Semen preparation and oocyte pickup, insemination, fertilization check
       and follow embryo development.

       -In charge of quality control and embryo freezing.



1987-1996: Centre for Early Human Development (CEHD), Monash University
and Monash IVF.

       -Semen analyses before patients start an IVF cycle.

       -Obtain patients’ consent for the treatment

       -Report on results to clinicians

       -Liaise between patients, clinicians and nurses and advice on the most
       suitable treatment for the patient on the day of the IVF procedure.

       -Involved in testicular biopsy analyses and worked closely with Dr. R.
       McLachlan and Prof D. DeKretser.

       -Sperm microinjection.



COLLABORATIONS:

Professor Carlos Simon MD, Ph.D. 2007, Professor of Obstetrics &
Gynaecology. Valencia University

Scientific Director of Instituto Valenciano de Infertilidad (IVI) Head of Valencia
Stem Cell Bank.

Prince Felipe Research Centre (CIPF). Collaborative work on the derivation of
germ cells from human

Stem cells.




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Professor Karim Nayernia, 2007, Chair of Stem Cell Biology, Institute of
Human Genetics, University of Newcastle, International Centre for Life,
Central Parkway Newcastle, UK. Collaborative work on germ cell derivation
from stem cells



Dr. Janet Rossant, 2003, from the Samuel Lunenfeld Research Institute,
University of Toronto, Canada: Provided trophectoderm stem cells for the
research on differentiation of germ cells from embryonic stem (ES) cells.



Dr. Peter Mountford, 2003, from Stem Cell Sciences, Melbourne, Australia:
Provided ES cells carrying the Lac-Z marker gene for the study on
differentiation of germ cells from embryonic stem (ES) cells.



Dr. Toshiaki Noce, 2003, from Mitsubishi Kagaku Institute of Life Sciences,
Japan: Provided the mouse antibodies for identification of VASA expression in
germ cells.



Dr. Yoel Shapiro from Hadassah Jerusalem/The Hebrew University, Israel,
visiting scientist in 2003 for 7 months. Research topic: Study of the regulation
of imprinted genes following somatic nuclear transfer by the analysis of
replication synchrony.



Prof. Lawrence Lewin from the Faculty of Medicine, Tel-Aviv University, Israel,
visiting scientist in 1997 for 3 months. Research topic: Real time of mouse
sperm decondensation analysis using propidiume iodide with Confocal
microscopy following in-vitro fertilization.



Dr. Yoshinory Ohkuma from the Kurume University School of Medicine,
Japan, in 1996/97 for 12 months. Research topic: Sperm cytosolic factor- its
identification and participation in oocyte activation and fertilization




PUBLICATIONS:



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Refereed Journals

      1.     O. Lacham, A. Trounson C. Holden, J. Mann, H.
      Sathananthan (1989), Fertilization and development of mouse
      eggs injected under the zona-pellucida with single spermatozoa
      treated to induce the acrosome reaction. Gamete. Res. 23: 233-
      243.



      2.    Trounson, A. Peura and O. Lacham (1989), Fertilization of mouse
      and human eggs by microinjection of single spermatozoa under the zona
      pellucida. J. Reprod. Fertil. (Suppl.) 38:145-152.



      3.    I Kola, O. Lacham, R.P.S. Jansen, M. Turner and A. Trounson
      (1990), Chromosomal analysis of human oocytes fertilized by
      microinjection of spermatozoa into the perivitelline space. Hum. Reprod.
      5:575-577.




      4.     O. Lacham, and O. Trounson (1991), Fertilizing capacity of
      epididymal and testicular spermatozoa microinjected under the zona
      pellucida of mouse oocytes. Mol. Reprod. Devel. 29:85-93.



      5.   D. Sakkas, O. Lacham, L. Gianaroli, A. Trounson (1992), Subzonal
      sperm microinjection in cases of severe male factor infertility and
      repeated in vitro fertilization failure. Fert. Steril. 57:1279-1288.




      6.    A.H. Sathananthan, A. Trounson, A Peura, J. Mann, O. Lacham
      (1992), Fertilization in the mouse by single or multiple sperm injection:
      Ultrastructure. Asst. Reprod. Technol. Suppl III: 1-14.



      7.   O. Lacham-Kaplan and A. Trounson (1993), The effect of the
      sperm motility activator 2- deoxyadenosine and pentoxifylline used for
      sperm microinjection on mouse and human embryo development. Hum.
      Reprod. 8:945-952.


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      8.   R.I. McLachlan, G. Fuscaldo, I. Calderon, O. Lacham-Kaplan, C.
      Poulos, H. Rho, A. Trounson and C. Wood (1994), Microinjection: choice
      of embryo transfer techniques. Reprod. Fertil. Dev., 6: 57-61.



      9.     O. Lacham Kaplan and A. Trounson (1994), Embryo development
      capacity of oocytes fertilized by immature sperm and sperm treated with
      motility stimulants. Reprod. Fert. Dev., 6:113-116.



      10. O. Lacham-Kaplan and A. Trounson (1995), Mouse sperm fertilizing
      capacity following sub-zonal microinjection is dependent on sperm
      washing and response to solubilised zonae pellucidae. Zygote. 3: 1-16.



      11. FL Barnse, A Crombie, DK Gardner, A Kausche, O. Lacham-
      Kaplan, AM Suikkare, J. Tiglias. C. Wood, A. Trounson (1995):
      Blastocyst development and Birth after in-vitro maturation of human
      primary oocytes, intracytoplasmic sperm injection and assisted hatching.
      Hum. Reprod. 10: 3243-3247.



      12. O. Lacham-Kaplan and A. Trounson (1995), Intracytoplasmic sperm
      injection in mice: Increased fertilization and development to term after
      induction of the acrosome reaction. Hum. Reprod. 10: 2642-2649.



      13. O. Lacham-Kaplan and A. Trounson (1997): Fertilization and
      embryonic development capacity of epididymal and testicular sperm
      and immature spermatids and spermatocytes. Rep. Med. Rev., 6: 55-
      68.



   14. AH. Sathananthan, A. Szell, SC NG, A. Kausche, O. Lacham-Kaplan,
       AO Trounson (1997). Is the acrosome reaction a prerequisite for sperm
       incorporation after intracytoplasmic sperm injection (ICSI)? Reprod.
       Devel. Fertil. 9:703-709.




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   15. A. Trounson, O. Lacham-Kaplan, M. Diamente, T. Gougoulidis (1999).
       Reprogramming cattle somatic cells by isolated nuclear injection.
       Reprod. Fertil. Devel. 10: 645-650.




   16. A. McKinnon, O. Lacham-Kaplan, A. Trounson (2000): Pregnancies
       produced from fertile stallions by intracytoplasmic sperm injection
       (ICSI) of single frozen/thawed spermatozoa into in-vivo matured mare
       oocytes. J. Reprod. Fert. Supp. 56: 513-517.



   17. O. Lacham-Kaplan, M. Diamente, D. Pushett, V. Hall, I. Lewis and A.
       Trounson (2000): Developmental competence of nuclear transfer cow
       oocytes following direct injection of fetal fibroblast nuclei. Cloning: 2:2-
       7.



   18. E. Hayes, S. Galea, A. Verkuylen, M. Pera, J. Morrison, O. Lacham-
       Kaplan, A. Trounson (2001): Nuclear transfer of normal and genetically
       modified somatic cells in the rat. Physiological Genomics 5: 193-203.



   19. K.M. Robertson, E. Simpson, O. Lacham-Kaplan, M. E. E. Jones
       (2001): Characterization of the fertility and sexual behaviour of the
       male aromatase knockout (ArKO) mouse. J. Of Andrology J. of Androl.
       22: 825-830.



   20. N. Korfiatis, A. Trounson and O. Lacham-Kaplan (2001) Effects of donor
       cell cycle on in-vitro development of bovine nuclear transfer embryos
       produced by Piezo injection. Cloning, 3: 125-138.



   21. O. Lacham-Kaplan, R. Daniels, A. Trounson (2001) Fertilization of
       mouse oocytes using somatic cells as male germ cells. Reproductive
       Biomed. Online, 3:205-211.



   22. RT Tecirlioglu, O. Lacham-Kaplan and A. Trounson (2003): Live
       offspring from electro ejaculated mouse sperm. Reprod. Fertil. Devel.
       14: 471-478.

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   23. O. Lacham-Kaplan, J. Shaw, LG. Sanchez Partida and A. Trounson
       (2003): Oocyte Activation After Intracytoplasmic Injection With Snap-
       Frozen Sperm Results in Live offspring From Inbred and Hybrid Mouse
       Strains. Biol. Reprod. 69: 1683-1689.



   24. Lacham-Kaplan (2004): In-vivo and in-vitro differentiation of male
       germ cells in the mouse. Reproduction, 128:147-152.



   25. Lacham-Kaplan, H Chy and A Trounson (2006): Testicular cell
       conditioned medium supports differentiation of embryonic stem cells
       into ovarian structures containing oocytes. Stem Cells 24: 266-273.



   26. L Jean and O Lacham-Kaplan (2006): The employment of strontium to
       activate mouse oocytes: effects on spermatid-injection outcome.
       Reproduction131: 259-267.



   27. TJ Wilson, O Lacham-Kaplan, J Gould, A Holloway, I Bertoncello, PJ
       Hertzog, A Trounson. (2007): Comparison of mice born after
       intracytoplasmic sperm injection with in vitro fertilization and natural
       mating. Mol Reprod Dev. 74:512-519.



   28. M West, O Lacham-Kaplan, M Cleary, D Galloway, J Shaw, AO
       Trounson, MC Paris. (2007) In vitro maturation and intracytoplasmic
       sperm injection of oocytes collected from hormonally stimulated
       common wombats, Vombatus ursinus. Anim Reprod Sci. 98:311-321.



   29. D Saiti and O Lacham-Kaplan (2007): Mouse germ cells development
       in-vivo and in-vitro. Biomaker Insight 2:241-252.



   30. D Saiti and O Lacham-Kaplan (2007): Density Gradients for the
       Isolation of Germ Cells from Embryoid Bodies. (Accepted by
       RbmOnline):



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   31. GM Jones, DS Cram, B Song, MC Magli, L Gianaroli, O Lacham-
       Kaplan, JK Findlay, G Jenkin and AO Trounson (2008): Gene
       expression profiling of human oocytes following in-vivo or in-vitro
       maturation. Hum Reprod (Accepted February 18th 2008)



   32. A Marques-Mari I, O Lacham-Kaplan, JV Medrano, A Pellicer, and C
       Simón (2008). Differentiation of Germ Cells and Gametes from Stem
       Cells. Human Reproduction Update (invited review-submitted).



   33. Lacham-Kaplan and K Nayernia (2008): Risks associated with
       therapies using in-vitro derived germ cells (Invited review-in
       preparation). Human Reproduction Update.




Invited Chapters in Books

   1. Trounson, C. Yates, P. Temple-Smith and O. Lacham (1989), The value
      of IVF for male infertility management, In; Perspectives in Andrology,
      Eds; M. Serio, Serono Symposia, Italy Raven Press, 53:421-429.



   2. O. Lacham-Kaplan and A. Trounson (1994), The need of micro assisted
      fertilization in human reproduction. In; Gamete and Embryo
      Micromanipulation in Human Reproduction. Eds. S. Fisher and M
      Symonds, Edward Arnold press, London p: 55-59



   3. A. Trounson, O. Lacham-Kaplan, L. Gianoroli (1994), Subzonal and
      intracytoplasmic sperm microinjection for treatment of severe male
      infertility In: Treating Male Infertility. Eds. G.M. Colpi, M. Balema, Prog.
      Reprod. Biol. Basel Karger 16:269-277.



      4.    O. Lacham-Kaplan and A. Trounson (1995), Micromanipulation
      assisted fertilization "Comparison of different techniques". In: Male Factor
      in Human Infertility. Ed. J. Tesarik, Serono Symposia, Rome, pp. 287-
      304.




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      5.   AZ. Szell, A. Kausche, K. May, O. Lacham-Kaplan, N. Lolatgis, J.
      F. Leeton and A. Trounson (1996): In vitro oocyte cultures: clinical
      aspects. Eds, M. Filicon and C. Flamigni, Elsevier Science B. V., pp 159-
      167.



      6.    Trounson, I. Gunn, O. Lacham-Kaplan, I. Lewis, A. McKinnon, T.
      Peura, J. Shaw (1998). Manipulation of development: Opportunities for
      animal breeding. In: Gametes: Development and Function. Eds. A.
      Lauria et al., Serono Symposia, Rome. Pp 485-499.



      7.   Lacham-Kaplan (2004): Haploidization of somatic cells following
      nuclear transfer into MII mouse oocytes. In. KA Rao, PA Brinster & AH
      Sathananthan (Eds), The Infertility Manual 2nd Ed. Jaypee, New Delhi
      pp 498-505 (ISBN 81-8061-251-1).



   8. Lacham-Kaplan and D Saiti (2007): Oocyte differentiation from
      embryonic stem cells. In Advances in Fertility Studies and
      Reproductive Medicine, Eds: Kruger TF, Van Der Spy Z and Kempers
      RD, Besins Healthcare, Durban, Pp 148-152.




Elected Abstracts presented in conferences:

      1.   Kola, S. Edmonson, J. Proudfoot and O. Lacham (1989), The
      role of heat shock protein (HSP-70) in mouse oocytes and pre-
      implantation embryos. Proc. Gene/Regulation/Oncogene 1:1.



      2.    I Kola, S. Edmonson, A. Davy and O. Lacham (1989), Heat shock
      protein (HSP-70) expression is necessary for the activation of the
      embryonic genome in the mouse. Proc. Organization and expression of
      the Genome. 11:1.



      3.   A. Trounson, O. Lacham, L. Wilton, E. Barnaby. and I. Kola (1989)
      Micromanipulation in IVF: Basic and clinical advances. Cell Diff. and
      Devel. 27:34.



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      4.   O. Lacham and A. Trounson (1989), Epididymal sperm function in
      the mouse assessed by microinjection under the zona pellucida of the
      oocytes. Proc. Aus. Soc. Rep. Biol. 21:86.



      5.      O. Lacham, D. Sakkas and A. O. Trounson (1990), improved
      fertilization rates after microinjection of human male factor sperm under
      the zona pellucida. Proc. Fert. Soc. Aus. 25.



      6.    P.W. Sobieszczuck, S. Edmonson, O. Lacham and I. Kola (1990),
      Murine ets-2 Oncogene in early embryo development. EMBO meeting,
      Mol. Biol. of Vertebrate development 16:96.



      7.    O. Lacham-Kaplan and A. Trounson (1991), fertilized mouse
      oocytes by immature spermatozoa have limited pre-implantation
      development in vitro. Proc. Aus. Soc. Rep. Biol. 23:41.



      8.    D. Sobieszczuck, G. Fuscaldo, O. Lacham and A. Trounson
      (1991), Sperm microinjection and results in PROST TEST and IVF-ET
      cycles. Proc. Fert. Soc. Aus. 26.



      9.   G. Fuscaldo, R. McLachlan, D. Sobieszczuck, O. Lacham, A.
      Trounson (1991), Prediction of microinjection fertilization outcome based
      on semen characteristics. Proc. Fert. Soc. Aus. 26.



      10. A. Trounson, O. Lacham (1993) Microinjection of sperm into ova.
      Proc. World Congress on IVF. 8.



      11. M.C. Valiotis, O. Lacham-Kaplan and A. Trounson (1993), Pronuclei
      formation and embryo cleavage following electrofusion of round
      spermatids with oocytes from the mouse. Proc. Aus. Soc. Rep. Biol.
      25:48.




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      12. O. Lacham-Kaplan, M.C. Valiotis and A. Trounson (1994),
      Development of mouse embryos after fusion of oocytes with testicular
      round spermatids. Theriogenology 41:232.



      13. O. Lacham-Kaplan, A. Trounson (1994), Effect of sperm motility on
      the fertilization rate by intracytoplasmic sperm microinjection. Proc. Aus.
      Soc. Rep. Biol. 26:21



      14. A. Crombie, A.M. Suikkari, A. Kausche, J. Tiglias, O. Lacham-
      Kaplan, F. Barnes, A. Trounson (1994), Endocrinology of cycles
      involving immature oocyte collection. Proc. Fert. Soc. Aus. 27:08



      15. A.H. Sathananthan, F.L. Barnes, A. Trounson, O. Lacham-Kaplan,
      A. Kausche, J. Tiglias (1994), Ultrastructure of human oocytes matured in
      vitro. Proc. Fert. Soc. Aus. 27:0137.



      16. A. Szell, A. Kausch K. May, O. Lacham-Kaplan, N. Lolatgis, JF
      Leeton, A. Trounson (1996), In-vitro oocyte cultures: clinical aspects.
      Symposium on The Ovary: Regulation, Dysfunction and treatment. Marco
      Iland, Florida (USA), Jan. 25-27.



      17. O. Lacham-Kaplan, T. Gougoulidis, X. Ji, and AO Trounson (1996):
      Expression of TEC4 antigen on mouse pre-implantation embryos
      obtained from immature epididymal sperm. 12th Annual Meeting ESHRE,
      30 June-3 July, pp. 60-61.



      18. A. Trounson, O. Lacham-Kaplan and Teija Peura (1996):
      Fertilization and Development: From gamete fusion to development
      independent of the paternal genome. Serono Symposia, November
      2nd, 1996, Hynes Convention Centre, Boston, USA.



      19. O. Lacham-Kaplan, Y. Ohkuma, M. Jones, T. Gougoulidis, X. Ji,
      A. Trounson (1997): Fertilization of mammalian oocytes with immature
      sperm. Prince Henry’s 5th Annual Symposium, March 19-21, 1997,
      Monash Medical Centre, Melbourne, Australia.


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                                    - 19 -




      20. O. Lacham-Kaplan, Y. Ohkuma, A. Trounson. (1997): Activation
      of mouse oocytes with human sperm extract increases fertilization
      rates after injection of immotile mature sperm and nuclei isolated from
      round spermatids. American Society for Reproductive medicine, 53rd
      Annual Meeting, October 18-22, Cincinnati, Ohio, USA.



      21. A. Trounson, O. Lacham-Kaplan, G. Jones (1997): Reproductive
      capacity in patients with gonodal dysfunction. The XV FIGO World
      Congress of gynaecology and Obstetrics. September, USA.



      22. O. Lacham-Kaplan and A. Trounson (1997), Growing embryos: do
      we really need a zona? In: ART: State of the art. Serono Symposia,
      December, Hawaii, USA.



      23. O. Lacham-Kaplan, Y. Okuma and A. Trounson (1997), Genetic
      and developmental consequences of using immature sperm cells:
      spermatids and spermatocytes. In: ART: State of the art. Serono
      Symposia, December, Hawaii, USA.



      24. J. Shaw, D. Mattiske, O. Lacham-Kaplan, G. Shaw (1998),
      Development of ovarian tissue from pouch young of a marsupial,
      Macropus Eugenii, after grafting to SCID mice. Gordon research
      conference, July 5-9, Boston, USA.



      25. BS. Choo, R. Glchrist, E. Spilseth, O. Lacham-Kaplan, O. Trounson,
      J. Shaw. (1998), Development of frozen thawed marmoset ovarian tissue
      after grafting to SCID mice. Australian Society for Reproductive Biology.
      August 23-26, Perth, Australia.



      26. O. Lacham-Kaplan, M. Diamente, O. Trounson (1999) Pregnancy
      following injection of mechanically isolated fetal fibroblast nuclei into
      enucleated bovine oocytes, Proc IETS, Theriogenology 51:206.




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                                    - 20 -


      27. M. Diamente, O. Lacham-Kaplan, O. Trounson (1999) Development
      of enucleated bovine oocytes, Proc IETS, Theriogenology 51:198.



      28. LM. Lewin, O. Lacham-Kaplan, L. Mittelman and A. Trounson
      (1999) Confocal microscopy of sperm chromatin decondensation in
      mouse eggs. Proc The Annual meeting of Israel Fertility Association (IFA)
      pp 25.



      29. WG James, O. Lacham-Kaplan, AO Trounson (1999) Successful
      development of 6-DMAP replacement for bovine oocyte activation
      research. ASRB, Melbourne, Australia.



      30. S. Galea, O. Lacham-Kaplan, AO Trounson (1999) confocal
      analysis of decondensation and pronuclear formation in human sperm
      injected into murine oocytes. ASRB, Melbourne, Australia.



      31. S. Galea, O. Lacham-Kaplan, AO Trounson (1999) preliminary
      study: high fertilization rates produced with prolonged storage of male
      factor patient sperm following ICSI into murine oocytes. ASRB,
      Melbourne, Australia.



      32. N. Korfiatis, A. Trounson and O. Lacham-Kaplan (2000) Effects of
      donor cell cycle on in-vitro development of bovine nuclear transfer
      embryos produced by Piezo injection ASRB, Canberra, Australia



      33. O. Lacham-Kaplan, M. Diamente, D. Pushet, V. Hall, I. Lewis and A.
      Trounson (2000) nuclear transfer by injection of mechanically and
      chemically isolated fetal fibroblast nuclei in the bovine. ASRB, Canberra,
      Australia



      34. O. Lacham-Kaplan, R. Daniels (2001): fertilization using cumulus
      cells as artificial gametes. ESHRE, Lausanne, Switzerland




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                                    - 21 -


      35. O. Lacham-Kaplan, Iannello R., Hertzog P. and Trounson A.
      (2002): Array Analyses and Phenotyping of KO Mice Produced by ICSI.
      ESHRE, Vienna, Austria



      36. O. Lacham-Kaplan, Chy H, Trounson A (2005): Differentiation of
      ES cells into ovarian like structures”. ESHRE Copenhagen , Denmark .



      37. O. Lacham-Kaplan and Krishnamoorthy K (2006) Agouti Yellow
      (Avy) mice: model for epigenetic modifications for assisted reproduction
      technologies" was oral presentation at the ESHRE 2006 Annual
      Meeting in Prague (18 to 21 June 2006).



      38. O. Lacham-Kaplan, M. Ayhan, K. Oliva, G. Rice, and AO.
      Trounson (2007): 2-Dimensional Fluorescence Difference Gel
      Electrophoresis (DIGE) analyses of protein differences in maturing and
      aged mouse oocytes. Keystone Symposia on Reproduction, Santa Fe,
      USA (20-25 February, 2007)



      39. D. Saiti and O. Lacham-Kaplan (2007) Isolation of Precursor germ
      cells from embryoid bodies by density gradient centrifugation. 5 th
      International Society for Stem Cell Research (ISSCR) Annual Meeting,
      Cairns, Australia (17-20 June, 2007)



      40. R. Acharya, A. Trounson and O. Lacham-Kaplan (2007) Age
      related reprogramming in mouse oocytes to support embryonic stem
      cell development. 5th International Society for Stem Cell Research
      (ISSCR) Annual Meeting, Cairns, Australia (17-20 June, 2007)



      41. O. Lacham-Kaplan and D. Saiti (2007): Efficiency of haploidization
      following ES cell derived germ cells injections into mature mouse
      oocytes. 23rd annual meeting of ESHRE, Lyon, France (1-4 July)




Invited speaker to scientific conferences and academic and medical
institutes:

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                                    - 22 -


      1.       “Sub -Zonal sperm microinjection as a treatment for male
      infertility” Beilinson Medical Centre, Petah Tikva, October 1989, Israel.



      2.    “Sub-Zonal sperm microinjection as a treatment for male infertility”
      Hakiria Medical Centre, Tel-Aviv, October 1989, Israel.



      3.     “The need of microinjection in human reproduction” The first
      international congress on micromanipulation in human reproduction,
      Rome, December 1990, Italy.



      4.  “Sperm microinjection program at Monash IVF”, Department of O &
      G, Gothenburg University , Gothenburg, November 1991, Sweden .



      5.      “Embryonic development after sperm microinjection” Serono
      satellite symposium on male infertility, Adelaide , November 1992,
      Australia .



      6.   “SUZI and ICSI at Monash IVF” Monash IVF-IVF America
      Symposium, Melbourne, March 1993, Australia.



     7. “Microinjection        assisted      fertilization: Comparison    of
     different techniques”, Serono International Symposium on Male Factor In
     Human Infertility. April 1994, Paris, France.



      8.   “Fertilization of mammalian oocytes by sperm precursor cells and
      immature sperm”, 5th Annual Symposium, Prince Henry’s Institute of
      Medical Research, March, Melbourne 1997, Australia.



      9.     “Genetic and development consequences of using immature sperm
      cells, spermatids and spermatocytes”, Serono International Symposium
      on ART: State of the Art”. December 1997, Hawaii, USA.




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                                   - 23 -


      10. “Genetic and developmental consequences of using immature sperm
      cells: spermatids and spermatocytes”. Serono International Symposium
      on ART: State of the Art”. December 1997, Hawaii, USA.



      11.. “Micromanipulation procedures for gametes and embryos”
      Australian Society for reproductive Biology (ASRB). September 1999,
      Melbourne, Australia.



      12. “ From germ cells to somatic cells” dream or reality. The Israeli
      Fertility Society (IFS), April 2002, Tel-Aviv, Israel.



      13. “Fertilization – Science and Fiction in the IVF Laboratory” Pre
      Congress Workshop on Embryology & Laboratory IVF Protocols:
      August 2004, Kajurahoo, India Supported by Vardhaman Medical, New
      Delhi, In Vitro Care Inc., USA & Cryos, Denmark.



      14. 2006: “Controlled oocyte differentiation from murine ESC” the
      Serono conference on germ cells, March 2006, Valencia, Spain.



      15. “Oocyte differentiation from embryonic stem cells” The
      International Federation of Fertility Society (IFFS) 2007. XIX World
      Congress on Fertility and Sterility, April 2007, Durban SA.



   16. “Epigenetic stability in IVF/ICSI and embryo culture” PGDIS June 2007,
       Melbourne, Australia.



   17. “Stem Cells to Germ Cells (Male and Female). Fertility Society of
       Australia (FSA), September, 2007, Hobart , Australia ,




TEACHING AND TUTORING:

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                                      - 24 -




2003 Demonstrating micromanipulation procedure in “Frontiers in Human
Embryonic Stem Cells” advanced training course at the University of Pittsburgh,
USA.



2002 Coordinator, lecturer and laboratory tutor: Master of Clinical Embryology
(MCE): Practical Techniques & Statistics in Embryology 1 & 2, Monash
University. Lecturer: Graduate Diploma of Reproductive Sciences, Monash
University.



1999-2001 Co-coordinator (with Prof. A. Trounson), lecturer and laboratory tutor:
Master of Clinical Embryology (MCE): Practical Techniques & Statistics in
Embryology 1 & 2. Monash University. Lecturer: Graduate Diploma of
reproductive sciences, Monash University.



1984-1987:                    Instructor in student laboratories:

Comparative Anatomy, Histology and Endocrinology, Zoology Department,
Tel-Aviv University.

Lectures given at the MCE course:

                    on (1999)


                                -2002)
Practical sessions given at the MCE course:
                           -2002)
                                         -2002)
                       -2002)
Lectures and presentations given to Graduate Diploma of Reproductive
Sciences Course:
* Fertilization in-vitro and ICSI with ejaculated sperm and epididymal sperm
(lecture and practical) (1999-2002)
                                                               -2002).



Additional obligations in teaching and coordinating: Providing syllabus for
lectures and preparing exam questions, marking students on practical and

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                                     - 25 -


written exams, assessing student oral presentation, following student progress
in practical sessions, reading and commenting on student reports and
laboratory protocols.

Teaching methods: Lectures, using power-point presentations, video
sessions and discussion of scientific articles. All teaching material is prepared
and presented by Orly Lacham-Kaplan.



SUPERVISION:



2007/8: Deshira Siati: Ph.D. “Production of oocytes from mouse embryonic stem
cells”. Main Supervisor: Dr. O. Lacham-Kaplan. Co-Supervisor: Dr. Gayle
Jones. On going.



2007/8: Rutu Acharia. Master of Biomedical Sciences Part 2: “Formation of ES
cells from young and aging mouse oocytes”. Main Supervisor: Dr. O. Lacham-
Kaplan. On-going



2007/8: Anusua Chanda. Master of Biomedical Sciences Part 2: “Production of
ES cells from mouse embryos”. Main Supervisor: Dr. O. Lacham-Kaplan. On-
going



2006/7: Rutu Acharia. Master of Biomedical Sciences Part 1: “Nuclear transfer
of somatic cells into young and ageing mouse oocytes”. Main Supervisor: Dr. O.
Lacham-Kaplan. Completed/awarded in 2007.



2006: Paul McNamara: Master of Biomedical Sciences Part 1: “Identification
of epigenetic modifications in embryos”. Main Supervisor: Dr. O. Lacham-
Kaplan. Completed /awarded in 2007.



2005: Deshira Siati: Master of Reproductive Sciences “The isolation of mouse
precursor germ cells from embryonic stem cell derived embryoid bodies” Main
Supervisor: Dr. O. Lacham-Kaplan. Completed/awarded in 2007.




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2005: Thilini Samarasinghe. Bachelor of Biomedical Science Honours:
“Fertilization and pre-implantation development of oocytes obtained from aged
obese females”. Main Supervisor: Dr. O. Lacham-Kaplan. Completed/awarded
in 2006/7.



2004: Kalyani Krishnamoorthy: Master of Reproductive Sciences
“Developmental competence of Agouti mice embryos produced by in-vitro
fertilization and intracytoplasmic sperm injection” Main Supervisor: Dr. O.
Lacham-Kaplan. Completed/awarded in 2006.



2003: Jean Loren: Master of Reproductive Sciences “The employment of
strontium to artificially activate oocytes” Main Supervisor: Dr. O. Lacham-
Kaplan. Completed/awarded in 2005.



2002: Sivachelvi Kanagasabai Master of Reproductive Sciences “In-vitro and in-
vivo development of mouse embryos following in-vitro fertilization and
intracytoplasmic injection of fresh and suboptimal sperm using different culture
media” Main Supervisor: O. Lacham-Kaplan. Completed/awarded in 2003.



2002: Shai Marom: Work experience student, Melbourne High, N/A. Main
Supervisor: O. Lacham-Kaplan.



1999: Natasha Korfiatis Master of Reproductive Sciences “Donor cell cycle
phase: effects on development in bovine somatic cell nuclear transfer embryos”
Main Supervisor: O. Lacham-Kaplan. Co-supervisor: Prof Alan Trounson.
Completed/awarded in 1999.



1998: Sandra Galea, Honours “Sperm analysis using propidium iodide with
confocal microscopy and fluorescent in-situ Hybridisation (FISH)”
Completion/awarded in 1998. Main Supervisor: O. Lacham-Kaplan.



1998: Will James, Master of Reproductive Sciences “Intracytoplasmic sperm
injection in the bovine: a model for endangered species” Main Supervisor: O.
Lacham-Kaplan. Co-supervisor: Prof Alan Trounson. Completed/awarded in
1999.


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                                    - 27 -




1998: David Pushett. PhD. “Assisted reproduction and subsequent embryo
development to form embryos for transfer and cryobanking in endangered cats
using the domestic cat as a model” Main Supervisor: O. Lacham-Kaplan. Co-
Supervisor: Dr. Ian Gunn. Completed/awarded in 2003.



1998: Mulyoto Pangetsu. PhD “Drying biological material for use in assisted
reproductive technology” Main Supervisor: Dr. Jill Shaw. Co-Supervisor: O.
Lacham-Kaplan. Completed/awarded in 2003.



Informal Teaching:

Production of an educational video in collaboration with ProBio Int. on sperm
injection and nuclear transfer procedures.



Peer review involvement and memberships in societies and committees:



Grants:

2005: INSTITUTO PASTEUR FONDAZIONE CENCI                       BOLOGNETI;
UNIVERSITA DI ROMA LA SAPIENZA (ITALY- Institutionnel)



2005: THE ISRAEL SCIENCE FOUNDATION (Israel- Governmental)



2006: BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH
COUNCIL (UK- Commercial)



2007: AUSTRALIAN RESEARCH COUNCIL (ARC)




Scientific journals:


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                                    - 28 -


I regularly review papers on reproduction, somatic nuclear transfer and stem
cells for the scientific journals:




       Reproduction



   * Theriogenology



Membership in Scientific Societies:

       The Australian Society of Medical Research (ASRM)
       The Society of Reproductive Biology (SRB)
       The International Society for Stem Cell Research (ISSCR)
       The ESHRE stem cell interest group.



Membership in interest groups:




PAST and CURRENT EXTRACURICULAR ACTIVITIES



        * Deputy/ Acting chair SOBS animal ethic committee (AEC)
        member category B (Scientist)

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      Giving lectures to activity groups, high school students, parent groups
      and teachers.

                                                    PR (organising the
      MISCL station at the ISSCR conference, June 2007 and at the Monash
      University open day, 2007).



PUBLIC EDUCATION:

Lectures:

      1.   2001 “Cloning and fertilization” National Council of Jewish Women
      of Australia (Private organization, audience of 50 members).



      2.   2001 “Breakthroughs in fertility treatments”. Australian Friends of
      Tel Aviv University “Victoria. (Private organisation, audience of 100
      members)



      3.    2002 “Fertilization with somatic cells” The Jewish National Fund
      (Victorian Division) (Private organisation, audience of 60 members).



      4.    2002” IVF and Jewish ethics” The Guides of Jewish Museum of
      Australia (Private organisation, audience of 50 members)



      5.     2005 “Recreation in the dish” Royal Children’s Hospital. Australia
      (Institutional)



      6.  2005 “Advanced research in infertility and stem cells” Betar Youth
      Movement. (Private organisation, audience of 50, 16-20 year old
      members).



      7.   2007” Stem cell and reproduction” Ronald McDonald House AGM
      meeting, 9th October (50 members).

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      8.  2007 “In-vitro fertilization” U3A-`Caulfield, 10th October (25
      members)



      9.    2007” Stem cell and Reproduction” The King David School, Vic,
      15th October (>50 members).




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