Grant_Support_Doc_Neon by ashrafp


									Neon™ Transfection System
Manufacturer: Invitrogen Corporation, Carlsbad, CA
Part Numbers: MPK10096, MPK1096, MPK5000, MPK5000S, MPP100, MPS100, MPSERV, MPT100

The Neon™ Transfection System provides fast and efficient delivery of nucleic acids or siRNA
into majority of mammalian cell types, including primary, stem, and difficult to transfect cells
(DTTC). The flexible and open systems allows the user to perform high-quality transfections
using optimized or user-designed protocols in three simples steps with as little as 2 x 104 cells per
reaction. Unlike standard cuvette-based electroporation chambers, the Neon™ system uses a
patented biologically compatible pipette tip chamber that generates a more uniform electric field.
This design allows a better maintenance of physiological conditions resulting in very high cell
survival compared to conventional electroporation. The transfection occurs in the Neon™ Pipette
tip. .

The Neon™ Transfection technology was designed for highly efficient gene delivery and ease of
use. With one ‘fail-safe’ optimization experiment, you can maximize delivery efficiency and
minimize toxicity in any cell type. The transfection protocol is a simple 3-step protocol; mix cells
and plasmid or siRNA into the Neon™ Pipette tip, plug into the pipette holder, and press start.
Then transfer the transfected cells directly into your culture vessel. The Neon™ cell database
offers tested protocols for a variety of cell lines or use the 24-well optimization protocol as a
starting point for a new cell type. .

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