Extraction of yeast cell lysate using liquid nitrogen and Waring blender by nuhman10

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									                              MIB General Risk Assessment Form

Date:            Assessed by:            Validated by:              Location:                                                    Review date:
01/05/09         Abi Stevenson                                      2.046 (McCarthy Lab)                                         01/05/10
                                                                    2.054 (3rd floor darkroom)

Task :

Extraction of yeast cell lysate using liquid nitrogen and Waring blender


Activity             Hazard            Person(s) in         Existing measures to control risk                      Risk rating            Result
                                       danger
                     Release of        Staff and those in   All staff should be trained in the correct handling and low                     A
Transport /          liquid nitrogen   close proximity      transport of liquid nitrogen. They should have
handling of liquid   – risk of                              completed COSHH and risk assessments so that they
nitrogen             asphyxiation                           are aware of the hazards associated with the
                                                            handling of liquid nitrogen.
                                                            Oxygen monitors and alarms are present on the
                                                            room, and are tested regularly to ensure they are
                                                            functional.
                                                            Warning signs and emergency advice are prominently
                                                            displayed.
                                                            Two personnel to be present when large volumes of
                                                            liquid nitrogen are dispensed.
                                                            No personnel to enter room when oxygen alarm
                                                            sounds. Staff to evacuate room when alarm sounds
                                                            continuously
                                                            Staff are forbidden from travelling in the lift with liquid
                                                            nitrogen. A warning sign must be displayed to warn
                                                            others not to enter the lift during liquid nitrogen
                                                            transport.
Activity         Hazard             Person(s) in      Existing measures to control risk                         Risk rating   Result
                                    danger
                 Release of         Staff and other   All staff who handle/use liquid nitrogen should have medium               A
                 liquid nitrogen    building users    attended a chemical safety course which details the
                 – risk of                            risks associated with liquid nitrogen, and what to do in
                 frostbite / cold                     case of a burn injury.
                 burns                                Protective clothing (lab coat, thermal gloves and
                                                      glasses) are available and should be worn. Avoid
                                                      exposing skin to liquefied gas.
                                                      Use suitable lidded storage containers with proper
                                                      insulation. Do not dispense into or transport in a non-
                                                      insulated container.
                 Mechanical         Staff             All equipment and power supplies are safety tested       low              A
Use of blender   /Electrical                          and regularly maintained.
                 hazard
                 Moving blades      Staff             Blender is to be used with the lid, do not put fingers    Medium          A
                 hazard                               into blender. Items to be removed from blender when
                                                      it is disconnected from the power supply and with a
                                                      spatula not fingers.
                 Eruption of        Staff             Wear safety specs. Keep one hand on top of blender        low             A
                 frozen yeast                         at all times and other hand near off switch. Switch off
                 out of blender                       if yeast erupts out of blender. Work to be carried out
                                                      in 2.054 away from other lab benches. Any yeast that
                                                      comes out of blender must be cleared up according to
                                                      GM risk assessment.




Authorisation by PI
I confirm that I have considered and understand the experiment and the associated hazards. I am
satisfied that all of the hazards have been identified and that the control measures to be followed will
reduce the risks to acceptable levels.
Print name:                                               Signed:

Date:


Declaration by researcher

I confirm that I have read this Risk Assessment and that I understand the hazards and risks involved and
will follow all of the safety procedures stated.


Declaration by PI

I confirm that the researcher who has signed below is competent to undertake the work. My counter-
signature indicates that I am happy for the work to proceed.


Name (please print)         signed                         PI countersignature               date

								
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