TWO Clicker at the start of class by mikeholy

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									              TWO Clicker questions at the start of class

• 1. Autoclaving and its consumer equivalent, pressure cooking, are
  methods designed to destroy which one of the following components of
  bacterial cells? Hint: these components are most often found in members
  of the two genera, Clostridium and Bacillus.

• 2. Although many antibiotics affect bacterial cell walls (the most desirable
  “target”), quite a few commercially available antibiotics affect other
  components of bacterial cells. Although these other antibiotics are more
  risky, as the bacterial cell “targets” are often similar to human cell
  components, they are still employed when dealing with various antibiotic-
  resistant bacteria. For example, which one of the following antibiotics has
  a unique bacterial DNA gyrase as its target?


• Powerpoint slide added (High frequency recombination) –
  COPIES are on ends of tables in the classroom
              TWO Clicker questions at the start of class

• 1. Autoclaving and its consumer equivalent, pressure cooking, are
  methods designed to destroy which one of the following components of
  bacterial cells? Hint: these components are most often found in members
  of the two genera, Clostridium and Bacillus.

• 2. Although many antibiotics affect bacterial cell walls (the most desirable
  “target”), quite a few commercially available antibiotics affect other
  components of bacterial cells. Although these other antibiotics are more
  risky, as the bacterial cell “targets” are often similar to human cell
  components, they are still employed when dealing with various antibiotic-
  resistant bacteria. For example, which one of the following antibiotics has
  a unique bacterial DNA gyrase as its target?


• Powerpoint slide added (High frequency recombination) –
  COPIES are on ends of tables in the classroom
Autoclaving and its consumer equivalent, pressure cooking, are methods
designed to destroy which one of the following components of bacterial cells?
Hint: these components are most often found in members of the two genera,
Clostridium and Bacillus.


• A. Endotoxins (lipid portions of bacterial LPS)

• B. capsules (either carbohydrate or protein)

• C. endospores

• D. MRSA
Although many antibiotics affect bacterial cell walls (the most desirable
“target”), quite a few commercially available antibiotics affect other
components of bacterial cells. Although these other antibiotics are more
risky, as the bacterial cell “targets” are often similar to human cell
components, they are still employed when dealing with various antibiotic-
resistant bacteria. For example, which one of the following antibiotics has a
unique bacterial DNA gyrase as its target?


A. Doxycycline – often used to treat severe cases of acne

B. Vancomycin

C.   Tetracycline

D. Ciprofloxacin (also called “cipro” and was used extensively during the
   anthrax “scare”)
BONUS Clicker question
Which one of the following genera has species all of which are
anaerobic? The species in this genus form spores and cause diseases
such as botulism, tetanus and gas gangrene.

• A. Bacillus

• B. Escherichia

• C. Clostridium

• D. Streptococcus
                Clicker questions at the start of class
                  30 seconds to answer each question


• 1. Which one of the following is not a characteristic of members of the
  bacterial family, Enterobacteriaceae?

• 2. Which one of the following plasmids would not be capable of carrying
  out conjugation?
Which one of the following is NOT a characteristic of members of the
bacterial family, Enterobacteriaceae?


• A. that are facultative anaerobic (i.e., can function in the presence or
  absence of oxygen)

• B. they contain endospores (thus they are very difficult to eradicate from a
  contaminated water supply)

• C. the are “bacillus” in shape

• D. they would stain red after the gram stain
Which one of the following plasmids would not be capable of carrying out
conjugation?

• A. an E. coli pBR322 plasmid

• B. an F+ plasmid

• C. a plasmid that had a tra nucleotide sequence and an origin of transfer
  (oriT) nucleotide sequence

• D. NONE of the above plasmids would be capable of carrying out
  conjugation
BONUS Clicker question
Almost all antibiotic genotypes reside in bacterial plasmids. However, a very limited
number of genotypes are actually within the chromosome. Which one of the
following is an example of a chromosomal antibiotic genotype?



• A. ampicillin

• B. aztreonam

• C. tetracycline

• D. doxycycline
             TWO Clicker questions at the start of class


• 1. High frequency recombination (Hfr) is best described by which one of
  the following?

• 2. Pasteurization of liquid food products is designed to do which one of
  the following?
High frequency recombination (Hfr) is best described by which one of the
following?

• A. conjugation using a pBR322 plasmid

• B. a form of conjugation using an F+ plasmid that has at least one insertion
  sequence (IS)

• C. the process in which most antibiotic resistance is naturally transferred
  from one bacterium to another, e.g., antibiotic resistance to ampicillin and
  tetracycline is most commonly transferred by Hfr

• D. a genetic process that is inhibited by carbapenamase
Pasteurization of liquid food products is designed to do which one of the
following?


• A. eliminate all enterohemorrhagic E. coli from apple cider and other fruit
  beverages

• B. destroy all endospores, including those of Bacillus anthracis and all
  species of the genus, Clostridium

• C. kill all pathogens in the food product that is being pasteurized

• D. kill all microorganisms in the food product that is being pasteurized
                     BONUS Clicker Survey
                any answer will receive full credit

• All incoming RIT students should be required to be tested for
  HIV infection before they are allowed to start classes. Each
  student tested will be informed of his or her HIV status (i.e.,
  infected or not infected)


• A. Yes

• B. No
• New York State law requires the reporting of the name of any
  HIV-positive person to the state Department of Health,
  regardless of whether or not that person gave permission for
  his or her name to be reported.
                     BONUS Clicker Survey
                any answer will receive full credit

• All incoming RIT students should be required to be tested for
  HIV infection before they are allowed to start classes. Each
  student tested will be informed of his or her HIV status (i.e,
  infected or not infected).


• A. Yes

• B. No
                TWO Clicker questions at the start of class
•   1. “Carriers” are a rather troublesome problem in public health, as this makes the
    monitoring and subsequent control of the spread of an infectious disease very
    difficult. Which one of the following would be the best description of carriers as
    this term applies to infectious diseases?

•   2. The antibiotic, doxycycline (a semi-synthetic tetracycline), is often used to treat
    severe cases of skin acne. This same antibiotic is quite effective in treating adults
    who are showing the early symptoms of which one of the following diseases that is
    caused by an enteric pathogen?


•   Tuesday Lab Section – put your three “environmental” TSA plates into the box on
    the front table before you leave the classroom. Make sure your name is
    somewhere on the plates (or tape) and the three plates are taped together (there
    is labeling tape available on the table). These plates will be incubated both at 37oC
    and room To, then returned to you in lab next week. Wait for at least 48 hours
    (i.e., at least until tomorrow) before viewing normal flora plates to give the skin
    flora time to grow enough. You can even wait until Monday without affecting your
    ability to observe and describe selected colonies. Keep normal flora plates in cold
    room after viewing them. They will be scored and used in lab next week.
“Carriers” are a rather troublesome problem in public health, as this makes
the monitoring and subsequent control of the spread of an infectious disease
very difficult. Which one of the following would be the best description of
carriers as this term applies to infectious diseases?


•   A. the transport and spread of endospores by major airline carriers from an
    isolated location to a very wide range of locations via shipments of medical
    supplies contaminated with methicillin-resistant Staphylococcus aureus

•   B. persons who are carrying a human pathogen in or on their bodies but do not
    show any symptoms of any related disease, yet are able to pass that pathogen
    onto another person who may actually then contract that disease

•   C. the main cause of disease pandemics, such as the recent H1N1 pandemic

•   D. any harm caused to the gastrointestinal tract by enterotoxin-carrying enteric
    bacteria such as Vibrio cholerae
The antibiotic, doxycycline (a semi-synthetic tetracycline) is often used to
treat severe cases of skin acne. This same antibiotic is quite effective in
treating adults who are showing the early symptoms of which one of the
following diseases that is caused by an enteric pathogen?


• A. mono (caused by the Epstein-Barr virus [EBV])

• B. cholera (caused by Vibrio cholerae)

• C. urinary tract infections (caused by Klebsiella pneumoniae)

• D. endocarditis (caused by ampicillin-resistant Enterobacter cloacae)

								
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