Gram Positive Cocci S. aureus M. roseus M. luteus S. ureae L. lactis S. aurantiaca Gram Positive Rods B.cereus B. subtilis M. smegmatis C. pseudodiphtheriticu m Gram Negative Rods E. coli P. vulgaris P. fluorescens C. freundii E. aerogenes K. pneumoniae A. viscolactis S. marcescens Gram Negative Cocci M. catarrhalis N. sicca
Kligler’s Iron H2S A/A A/A Alk/Alk Alk/Alk
Litmus Milk Alkaline Alkaline Alkaline Alkaline Acid, red, coag. Alk/pep, clot
Mannitol Salt + -
Alk/A
Alk, coag Alkaline Alkaline Alkaline
A/A, Gas Alk/A, H2S Alk/Alk Alk/A, gas Alk/A Alk/A
Acid, slight reduct. Alkaline Alkaline Everything Acid Red, pep, clot Acid, pep, coag. Everything
A/A
Alkaline Alkaline