Blood Sampling Tool for Free-Ranging Whales by liuhongmei

VIEWS: 8 PAGES: 15

									Blood Sampling Tool for Free-
      Ranging Whales




           Researchers:
   Brad Baum & Rajit Chakravarty
          Presentation Overview

• Design Problem and Specifications
• Background
       • Anatomy and Behavior
       • Current Techniques and Equipment
•   Design Ideas
•   Proposed Solution and Why
•   Future Problems
•   Questions
           Design Problem
• Estrous cycles & mating behavior are
  unknown
• Hormones in blood are crucial to
  understanding behavior & cycles
• No current blood extraction technology
• Design Problem
      - Blood obtaining instrument needed
   Project Design Specification
            Summary
• Requirements:
     - Collect 1cc of uncontaminated blood
     - Retrievable sample
     - Minimal harm to whale
     - Accurate and reliable
     - Compatible with crossbows
        Anatomy & Behavior

• ~ 15 meters in length
• Thick and strong skin
• Surface for 3-4
  breaths after 10-45
  minute dive
• Major blood vessels
  located in tail
           Existing Technology

• Biopsy darts
       - little blood
       collected
       - fired using a
       high-powered
       crossbow
• Contacted other
   researchers
         Suction Cup Solution
• Recommended by
  researcher
• Abrasive to skin of
  whale
• Absorbs blood
• Dissolvable plugs
                   Pros/ Cons
        Advantage              Disadvantages
• Not partial to blood   • No blood collection
   vessels                  method
                         • Tracking of whale
                            required
                         • Harmful to whale
                         • Not feasible
Needles & Vacuum Tubes
           • Used on humans
           • Needles
                 - vary in length
                 and thickness
           • Vacuum Tubes
                 - self sealing
                 - glass/ plastic
                 - rapid filling
  Single Needle Vacuum System
• Double sided ten
  gauge wide bore
  needle
• 2.5 inches in length
• Plastic vacuum tube
• Utilizes momentum to
  puncture seal
• High strength fishing
  line
                 Pros/ Cons
        Advantages                Disadvantages
• No contamination         • Small chances of
• Rapid blood extraction      hitting blood vessels
• Minimal harm to whale    • High chances of
• Easily retrievable          breaking
                           • Must penetrate thick
                              skin
Multiple Needle Vacuum System
               • Similar to single
                 needle system
               • 7 needles
               • Varying lengths of
                 needles (1.5-2.5
                 inches)
               • Proposed idea
  Pros/ Reasons for Choice

• All the advantages of a single needle
   system
• Increased chance of blood collection
• Increased strength of needle system
• Fits majority of required specifications
    Future Problems to Consider
• Force required to puncture skin ?
• Flight path ?
• Maybe more harm to the whale ?
• Mechanical problems ?
            - needle extraction from seal
            - sufficient momentum
• Size of blood vessels ?
Question/ Comments

								
To top