LABORATORY PROCESSING 0F THROAT SWABS Objectives 1. To practice the skills of processing a throat swab 2. To be able to distinguish the significant growth from the non-significant growth on a culture plate. 3. To a make a presumptive identification of the isolate. Procedure 1. Clerically identify any errors on the request form and the specimen. 2. No gram smear is required for a throat swab. 3. Culture the throat swab on BA, CA and SDA. 4. Incubate the inoculated culture medias in their respective temperatures and atmospheric conditions. 5. Read culture plates after 24 hours of incubation. 6. Thoroughly complete the work sheet. Throat swab culture plate reading 1. Describe the colonial morphology and growth on the culture plates. 2. Distinguish the significant growth from the non-significant growth. 3. Make a presumptive identification of your bacterial isolate. 4. Complete the work sheet and report on your specimen request form. Questions 1. Why isn’t gram smear required for a throat swab? 2. If a gram stain was performed on the throat swab, what would be the expected grams morphology? 3. What are some pathogens expected to found on the throat swab? 4. Why is a SDA plate included in the culture of a throat swab? 5. Would Mycobacterium tuberculosis be a pathogen isolated from the throat swab? Explain your answer. 6. What could be some obstacles in deciding which is a pathogen or not a pathogen in a throat swab culture? 7. What are some opportunistic pathogens likely to cause throat infections? 8. If an opportunistic pathogen was causing the throat infection, how would this be indicated to you in the culture growth?