Biology Enzyme Lab - DOC by GloriaEdem

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									Measuring the Effect of Enzyme Concentration
Group: Gloria Edem, Joy Edem, Juvita Jose

Materials
          Liver Catalase                                              Stopwatch
          Hydrogen Peroxide (3%)                                      Reaction Chamber
          Test Tubes (10)                                             Tweezers
          100 ml Graduated Cylinder                                   Distilled Water
          50 ml Beakers (10)                                          Regular Water
          200 ml Beaker (1)                                           Water Baths
          300 ml Beaker (1)                                           Ice
          10 ml Graduated Cylinder                                    Filter Paper Discs
          Tape                                                        Paper Towels

Procedure
  I.       Setup:
           - Filter Paper discs were put on a paper towel
           - Water bath was made using a plastic pan
           - 200ml of hydrogen peroxide was poured into the 300ml Beaker
           - 100 ml of Catalase was put into a 200 ml beaker
           - The enzyme was diluted as follows:
             - 100% : 10 ml of Catalase + 0 ml of distilled water
             - 90% : 9 ml of Catalase + 1 ml of distilled water
             - 80% : 8 ml of Catalase + 2 ml of distilled water
             - 70% : 7 ml of Catalase + 3 ml of distilled water
             - 60% : 6 ml of Catalase + 4 ml of distilled water
             - 50% : 5 ml of Catalase + 5 ml of distilled water
             - 40% : 4 ml of Catalase + 6 ml of distilled water
             - 30% : 3 ml of Catalase + 7 ml of disti
								
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